Csor.00g241770 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g241770
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionMediator of RNA polymerase II transcription subunit 20
LocationCsor_Chr05: 11151368 .. 11153751 (-)
RNA-Seq ExpressionCsor.00g241770
SyntenyCsor.00g241770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCTAAAATGGTAAAACCCCGGAAACCCCCATCAAAAAAGTGCTCGTTATTTCTTGTGGATTTGTTTCTTGATCTCCTTTCTTTGTTGTTCTTTGATTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGGTGATCGCATTCTTTTTGTAGAGACGATTTTCGGGATTTGATTACCTAAATGAATCTTAAATCTGTCAACAACAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACCCACCCAGGCTTTTCTAGTTCTGATGCATTCTTCATTTCCCCCATGTCCAGAAATTCAAATTTGAAATTGATTTTTGTAGCTTTCTCGTCTCTGTTCTTCTGCCCTTTTGTTTATAGTCGTGTGAGATGGATAAATCTTTGGTTACAGACAGTTTGCCTTGTTGGTTTTGTAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGGTAAGGCTCATTTCATCTTTTCATGTCAACTCTGCTCATATGTTATTGCATGTTCTTCTTGTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTCTTGTTTTTGTATCTGAGCGAGCAGTTTCTTTTCCTATGAGCTGTTGGCCTCTGTGGTTTGTTTCCACGACTTTTCAGAATGGTATGGTATTGTCCACTTTGAGCGTAAGCTCTCATCGCTTTGCCTTTAGTTTCTCCAAAAGGCCTCGTATACCAATGGAGATGTATTGCCTTATAAACCAACCACTCCTTAATTAGCTGACGTGGGATTTCTATCCCAATAATCCTCCCCTCGAGCAAAGTACATCATAGAGCCTCCTCTAAGGTCTATAGAGCCCTCAAACAACCTTTCCTTAATTGAGGCTCGACTCCTTCTCTGGAGCCCTCGAACAAATTTCACCCTTTGTTTGACACTTGAGTAACTTTTGACTACATCTTTGAGACTCACAACTTCTTTATTCGACATTTGAGGATTCTATTGACAAGGGCATGACTCTGATACCACGTTAGGAACCACAACTCTCTACAATGGTATGATATTATCCACTTTGAGCATAAGCTCTCGTGACTTTGCTTTTGGTTTCTCCAAAATGTCTCGTACCAATGGAAATGTAGTTCCTTATAAACCCATGATGAACCCCTTAATTAATCGATGTGGGACTCCTCCCCCAACAATCCTCAACATTGTTCTCAACTCAACTCGAGAACAATGAAGTAGAAGGAAAAGGCATCAAATAACTAATCAGTAGGCTCTGTAGTGAAGGATCTTATGTTGAGAAGTAGTAAGATCTGATTAGGATATCTTTGCAAAAGCCTACGTGTAGTCATAGTTATGGAAGTTCGTTATTGTGATCTTCACGCTTTCTGGGTAAATTCCTCCCAAAGAGTTTATTAAGTTTAGAAAGTGTCTTCCAGTTCACCTGGATGCTTGATTATAATATGTAGCCTTTGCTTGGGCTCAATTCCCTAGTGTGGTGCTCATCCTGAAACAAATAAAGCAAAGAAAGATGCCAATTTCTCTCGTCTCAAAAACATTTTGCCATGTTCTTTATTTGAAACTTCTCTGAGATGGCTTGCTTTCATGCTGAAGTTGGAACCTTGACACTTGGTCTCTAGCAAATCTCGATTCAATTCTGTAATTTGTTTCGAAGAACATCATATTGGAAGTACTGTTGAATTTGGAAAGCAGGACTCATATTGAAGTAGAATCTGTATGTCAAATGTGCACACATGTTATACTTTTTGGCTGCTGTTTGTTGCTCTTCCAATGAAACATTTTGAACACTTGAGACCCTTCACTATTCCTATGTTTAAACCTGTATATTGCAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

mRNA sequence

ATGCCGCTAAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

Coding sequence (CDS)

ATGCCGCTAAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATACTCAACGAGGTTTCGCAGTGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACTCTTACATTCTACAAACCCATCTTAAGAGATACGAACATAGCAGCGGGCGAGTTCCCGCGCGACTTTCTTGGAATTTCTGTGCCGGAGCAACCAAACAAGTACTATCTCATAATTCGTGGCCAACGGATCGTGTTAGAAGCTGATTTTACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTACCAATTAGGGGATTTCCAACTAAGAGTTGGAAAAGTTGTTCCCAACAATTCCGAGAATTTGAGAGGAATTGTCATGGAGATTGAGTATCTGCCAATTTCTTCAATGGAGAAATCAAAACAGGTGATGGAGGAGTTTTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGCTCCCTACCTGGTCGTTTCATGCACATAGAACCCAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCGGTTCAGTATGCCAATGTGATGGCCCAGCTAATCGCTACTGTGCAAGCAGTTCAATCAGCCAGAAACTAG

Protein sequence

MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Homology
BLAST of Csor.00g241770 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 2.5e-89
Identity = 160/219 (73.06%), Postives = 191/219 (87.21%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+D      E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKD-QANQLE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+ +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

BLAST of Csor.00g241770 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.2e-80
Identity = 141/217 (64.98%), Postives = 180/217 (82.95%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+D    A E
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQA-E 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GIS+PE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP ++E +RG+VME+EYLPISSM  +K++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

BLAST of Csor.00g241770 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 54/196 (27.55%), Postives = 91/196 (46.43%), Query Frame = 0

Query: 22  LNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDFLGISVPEQPNKYYLII 81
           L E+++ +E + G+K        + Y   + D    +   PR+F  +   E+P K + + 
Sbjct: 13  LPEMTKRIEFLGGIKVSTGTINCSLYLERVPD---GSNLIPREFHLLLYDEKPKKCFTVC 72

Query: 82  RGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSENLRGIV 141
           R    ++E D  + +I+EK  SYK R        +Y+LGDF +RVG +V      +RGI+
Sbjct: 73  RD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGDFIIRVGPIVFRT--EIRGIL 132

Query: 142 MEIEY----LPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDY--- 201
           +E+EY     P++S     +++ EF          +   G F   EP    +  S Y   
Sbjct: 133 VEVEYTACSTPVTSPFNCTKLLSEFI---------NGNLGPFP--EPIQTSFDFSAYPTL 190

Query: 202 ---YTSQHTAVQYANV 208
              Y+  HT+ QY N+
Sbjct: 193 PKSYSDLHTSYQYVNL 190

BLAST of Csor.00g241770 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 446 bits (1148), Expect = 3.81e-158
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 435 bits (1119), Expect = 1.01e-153
Identity = 216/223 (96.86%), Postives = 220/223 (98.65%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS++VMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. NCBI nr
Match: KAG7030510.1 (Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434 bits (1116), Expect = 8.51e-153
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 6   VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 65
           VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF
Sbjct: 36  VLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGEFPRDF 95

Query: 66  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 125
           LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR
Sbjct: 96  LGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLR 155

Query: 126 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 185
           VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN
Sbjct: 156 VGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPN 215

Query: 186 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 216 FAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 253

BLAST of Csor.00g241770 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 429 bits (1104), Expect = 1.95e-151
Identity = 214/223 (95.96%), Postives = 217/223 (97.31%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 424 bits (1091), Expect = 1.87e-149
Identity = 210/223 (94.17%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 1.84e-158
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 1.84e-158
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 429 bits (1104), Expect = 9.42e-152
Identity = 214/223 (95.96%), Postives = 217/223 (97.31%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+QVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 9.04e-150
Identity = 210/223 (94.17%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

BLAST of Csor.00g241770 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 5.08e-149
Identity = 210/223 (94.17%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N  A E
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 168

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKS+Q+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 169 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 228

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 229 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

BLAST of Csor.00g241770 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 329.7 bits (844), Expect = 1.8e-90
Identity = 160/219 (73.06%), Postives = 191/219 (87.21%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+D      E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKD-QANQLE 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+ +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP +SEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

BLAST of Csor.00g241770 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 298.5 bits (763), Expect = 4.4e-81
Identity = 141/217 (64.98%), Postives = 180/217 (82.95%), Query Frame = 0

Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+D    A E
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQA-E 60

Query: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GIS+PE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP ++E +RG+VME+EYLPISSM  +K++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

BLAST of Csor.00g241770 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 82.0 bits (201), Expect = 6.5e-16
Identity = 42/68 (61.76%), Postives = 55/68 (80.88%), Query Frame = 0

Query: 152 MEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQ-YANVMAQ 211
           ME +++VMEEF E+W EA++K SLPG+F++ + NF E+GL D YT QHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 212 LIATVQAV 219
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPF42.5e-8973.06Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ... [more]
Q9M0S06.2e-8064.98Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ... [more]
Q54FL32.6e-0927.55Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium... [more]
Match NameE-valueIdentityDescription
XP_022946745.13.81e-158100.00mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata... [more]
XP_038889134.11.01e-15396.86mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca... [more]
KAG7030510.18.51e-153100.00Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argy... [more]
XP_004141069.11.95e-15195.96mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60... [more]
XP_008459711.11.87e-14994.17PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ... [more]
Match NameE-valueIdentityDescription
A0A6J1KEU11.84e-158100.00Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36... [more]
A0A6J1G4U31.84e-158100.00Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=... [more]
A0A0A0LHQ19.42e-15295.96Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365... [more]
A0A1S3CAW69.04e-15094.17Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G... [more]
A0A5D3CH025.08e-14994.17Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku... [more]
Match NameE-valueIdentityDescription
AT2G28230.11.8e-9073.06TATA-binding related factor (TRF) of subunit 20 of Mediator complex [more]
AT4G09070.14.4e-8164.98TATA-binding related factor (TRF) of subunit 20 of Mediator complex [more]
AT2G28020.16.5e-1661.76BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013921Mediator complex, subunit Med20PFAMPF08612Med20coord: 1..208
e-value: 4.2E-43
score: 147.3
IPR013921Mediator complex, subunit Med20PANTHERPTHR12465UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49coord: 6..217
NoneNo IPR availablePANTHERPTHR12465:SF0MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20coord: 6..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g241770.m01Csor.00g241770.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016592 mediator complex
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0003712 transcription coregulator activity