Csor.00g225360 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g225360
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionmetal tolerance protein 4
LocationCsor_Chr10: 5258042 .. 5261768 (+)
RNA-Seq ExpressionCsor.00g225360
SyntenyCsor.00g225360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGAGAACCGGATACGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGTTCTTCTCTCTCTTCCTGATTATCGTTCTCTTATCTCTTCTGTTTTTTTTTTGCTGTTTCAATTTCTTTCTGGTCGCTGAAAGAATGAAGATTATAAACGAGGAGAATTCTTGTTTTGTTTATGATTATTTCCTCTTTTTTCGCTTGTTCTTTAGTGTACTTCTGAGATGATTACGAAGGCGGAGGCAACGCTTAACTCTGTTTTATATCCTCTGTTCATTAGAGTGAAATCTGATTAACCTAAACTGTTTTTGATGCGAAATGTTCATTTCGAATGCTCGTTTTTCTCTTTTTTCTTTTTTTTTTTTTCATTGAGGTTGTCATCTTCTGAGTAATCGCCATCTTCAATGTTCTAGGGTGGAAGAACAGATACTACTGACGTATTGGTTTGTTATAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTATTGAAGGTCGTCTATGTTACCAACCATGAATTTAGTACACTAGATGAAGAATTTCGTGGCCGTTGGCTTATAATGAGCAGCATTAATTATGATATCACCAATTTTCTGCAGACTTAGTCCTAAATCAACTATAACAAGGAAAAGAAAACGAGATAGAAAATGAATTGTTGAAATTGGTATGGCCTCAATGCTGAGCATATATAAATCAAGAGCTGATTATGATTGTTTTTTGGTTTATAGGCTCTGTTCTGGTCCCTTATTCCAGACCTTGAATTCACTCTCTAGTGACAGTTTGCAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGTATCTCTTGTTCTTTAAATCCAGTGTGAAATTGCATTGATTTTCTCATTCAGCCATTTGAACCATGATCATCCGTAGGAATATCTAGTAATTTTGTTGTCTATCTACCTCATATTCCTGCTAAAAATGAAGGTTCTTTCTCAATTATAACAAGTTAAAGTATTGACATCCATAATTGATGGATATTACGCCTATGTCTTGTAACTAGCAGAGAGCAAGTCTGTGCAATTATCTATTCATTTTGTATTCTGGAATCTTTAATGGTTGAATCATCTTGGTCACGTCAAAATTTCATTCCAAGCACGATTGTATGATTTGGGTTCCTTTAACAATGTGGATAGCTAAACCATTTCAATTTCTTGTTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATCCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGTCCTCTGGCTTTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTATGCTAAGGTTGTTTAACCTACAATTATTATTATGCAATTGTTTTCAAATGAATAATTCTTCAATATACATGGCATAAATCCTTCCAATGCTATTTCCCAGGATCATTACTTTGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCCCTAGCCATATATACAATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGGTACATATTTACTCGCATTTTGTTCCCTTTCTCCTAAAATGAAAATTTCTGATGTTTGTTGGATATGCTAATCAGTCTTCTTTTTGGGAAATCCCATGATTCCTTTTTTCTTTTTCTTCTTTTAACTTTTACTGGGCATAACAAAATACTAAAACGACTAGATCCGGTAAGGAGTTTTATAATAATAAGTCTAACACCCATGGTTCTAAATGAAAATGTTCTTCATCGAGTTGCAGAGCGAAAAGAAGATATTGGTGCGACCGTTCTTGTGTTGTCATAGTTAATGTGATATGATTTAAAAAAAAAAAAGATTTGAAATAACTATATTTTTGTCATATACCTGAAGTAATTGTATGTTTGTAGCATTTGCAGTTTTGAATTGTGAGGCATTCTCTCATTTCATGATACTTGCATTTCCTCCAGCTTACAACTAACACGAGGTTAATTCTTATTGTAGTTTCACTCGTGGGGAAATCAGCCCCTCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCTGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTCAGCCATACTTTTTCTATCTTGCATTTAGTTTTTTTCGTTTATTGTCTTAGCACCATAATCGCCGCAATGTGATTTGCACATGATAACTTTACACTCTTAGATATAAGAACGAAATTGCTAACCTATTTTAAATTTGTAACTAATGGTTATTAATGACGAGCATATTTCTAAATTCATAAATTCATGTCTGCATGATTTTAAAACGATCTAAAAACAGATTTTATGATTTAAGAAACTCTCTTTTAACCGTCAAGCCCACAGTTAGTAGATATTGTTTTCTTTGGGCTTTCCCTTTCAAGTTTCCCCTTAAGATTTTTAGAACACAGTCTACTAGGGGATGTTTCCACACCCTTATAAAGAATGTTTCGTCCTTCTCTCTAGCCGATGTGGGGTCTCATAATCCACCCCCTTCGGGGTCCAACGTCCTTGTTGACACTCATTCCCTTCTCCAATCGATGTGAGACCCCCAATCCACCCTTTTTCGGGGCCCAGCGTCCTTACTGGCACACCGCCTCGTGTCCACCAACCTTCGGGGCTAAGCCTCATCACTGGCACATCACCCTATGTCTGGCTTTGATACCATTTGTAACAGCCAAGCCCAACCTAGCAGAGATTGTCCTCTTTTGGCTTTCCATTTCGGACTTTTCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGGGAGGTTTCCACACTCTTATAAACAATGTTTCGTTCTCTCCCGATGTGAGATCTCACACTTTTCCATATTGGCACCTCCTAATAAAAAGGTTGAAACAAATCTCTTAACCAACAACTATACTTGGTTGCCGTTTGATGGTTTAAACTTAACAGGAACATTGTTTCAAAAACTTATGTAATTAAGAATTTGATATGCTTATACTGACAACCAATCAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACGTTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAG

mRNA sequence

ATGGACGGAGAACCGGATACGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTATTGAAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATCCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGTCCTCTGGCTTTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTATGCTAAGGATCATTACTTTGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCCCTAGCCATATATACAATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGTTTCACTCGTGGGGAAATCAGCCCCTCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCTGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACGTTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAG

Coding sequence (CDS)

ATGGACGGAGAACCGGATACGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTATTGAAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATCCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGTCCTCTGGCTTTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTATGCTAAGGATCATTACTTTGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCCCTAGCCATATATACAATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGTTTCACTCGTGGGGAAATCAGCCCCTCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCTGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACGTTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAG

Protein sequence

MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISNYANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Homology
BLAST of Csor.00g225360 vs. ExPASy Swiss-Prot
Match: Q10PP8 (Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 4.8e-155
Identity = 279/379 (73.61%), Postives = 325/379 (85.75%), Query Frame = 0

Query: 31  RGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFE 90
           R  SV S+R EF+SRLP+KV   + D E P  +D S+S     GEK+YYE+Q ATL+SFE
Sbjct: 18  RRNSVGSMRGEFVSRLPKKVLDAV-DPERPSHVDFSRSKGLREGEKEYYEKQFATLRSFE 77

Query: 91  EVDSLVTSDCIDEEDS-EERAQEERAMKISNYANIVLLLLKIYATVRSGSIAIAASTLDS 150
           EVDS+  S+ + EED   E+ Q E AMKISNYAN++LL LKIYAT++SGSIAIAASTLDS
Sbjct: 78  EVDSIEESNVMSEEDDIAEQKQSEFAMKISNYANMILLALKIYATIKSGSIAIAASTLDS 137

Query: 151 LLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLI 210
           LLDL+AGGILWFTHL MK +N+YK+PIGKLRVQPVGII+FAAVMATLGFQV +QA E+LI
Sbjct: 138 LLDLMAGGILWFTHLSMKSINVYKYPIGKLRVQPVGIIIFAAVMATLGFQVFVQAVEKLI 197

Query: 211 QDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGL 270
            ++  + L+P Q  WL +IMI ATVVKL LWLYC++S N IVRAYAKDHYFDVVTNVVGL
Sbjct: 198 VNETPDKLTPVQLTWLYSIMIFATVVKLALWLYCRTSGNKIVRAYAKDHYFDVVTNVVGL 257

Query: 271 VAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTR- 330
            AA+LGD  +WWIDP+GAIALA+YTI NWSGTVWENAVSLVG+SAPPE+LQKLTYL  R 
Sbjct: 258 AAAVLGDMFYWWIDPVGAIALAVYTITNWSGTVWENAVSLVGESAPPEMLQKLTYLAIRH 317

Query: 331 HPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHL 390
           HP++KRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGE+LQIKIE+LPEVERAFVHL
Sbjct: 318 HPQIKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGESLQIKIEELPEVERAFVHL 377

Query: 391 DFECEHKPEHSILRRLPNT 408
           DFEC+HKPEH+IL +LP++
Sbjct: 378 DFECDHKPEHNILSKLPSS 395

BLAST of Csor.00g225360 vs. ExPASy Swiss-Prot
Match: Q9M2P2 (Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=3 SV=2)

HSP 1 Score: 529.6 bits (1363), Expect = 3.0e-149
Identity = 277/381 (72.70%), Postives = 324/381 (85.04%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDS 94
           V+S++S F + LP+K++S + D E+P  +D+S++      EK+YYERQ+ATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  -LVTSD--CID----EEDSEERAQEERAMKISNYANIVLLLLKIYATVRSGSIAIAASTL 154
            L  SD   ID    EED  ERA +E AM+ISN+ANI LL LKIYATV+SGSIAIAASTL
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALKIYATVKSGSIAIAASTL 149

Query: 155 DSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQ 214
           DSLLDL+AGGILWFTHL MK VNIYK+PIGKLRVQPVGII+FAAVMATLGFQVLL AAEQ
Sbjct: 150 DSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATLGFQVLLVAAEQ 209

Query: 215 LIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVV 274
           LI ++PSE ++  Q IWL +IM+SAT +KLVLW+YCKSSRN IVRAYAKDH+FDVVTNV+
Sbjct: 210 LISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAKDHHFDVVTNVL 269

Query: 275 GLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVT 334
           GLVAA+L +  +WW+DP GAI LAIYTI+NWSGTV ENAVSL+G+SAPPEVLQKLTYLV 
Sbjct: 270 GLVAAVLANAFYWWLDPTGAILLAIYTIVNWSGTVMENAVSLIGQSAPPEVLQKLTYLVM 329

Query: 335 RH--PEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAF 394
           R     +K VDTVRAYTFGVLYFVEVDIELPE+LPLKEAHAIGE+LQIK+E+LPEVERAF
Sbjct: 330 RQGGDNIKHVDTVRAYTFGVLYFVEVDIELPEDLPLKEAHAIGESLQIKLEELPEVERAF 389

Query: 395 VHLDFECEHKPEHSILRRLPN 407
           VHLDFEC HKPEHS+L  +PN
Sbjct: 390 VHLDFECHHKPEHSVLSTIPN 409

BLAST of Csor.00g225360 vs. ExPASy Swiss-Prot
Match: Q6Z7K5 (Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 5.0e-144
Identity = 269/415 (64.82%), Postives = 334/415 (80.48%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+   + +T LL G     GS R   L R  S  SLRS F+SRLP+KV+         
Sbjct: 1   MDGD---DRRTPLLGG---EGGSTRPPSLRRRDSARSLRSTFLSRLPDKVRGGGDPERPA 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLV-------TSDCIDEEDSEERAQEE 120
            ++DL+++   S+GEK+YYE+Q+ATLK FEEV++L         ++ ++ ED E++ Q E
Sbjct: 61  ADVDLTRAKGLSQGEKEYYEKQLATLKIFEEVEALCMPGEFESDAEVLELEDKEQK-QSE 120

Query: 121 RAMKISNYANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYK 180
            AMKISNYANI+LL+ K+YAT+++GS+AIAASTLDSLLD LAGGIL+FTHL MK VNIYK
Sbjct: 121 SAMKISNYANIILLVFKVYATIKTGSMAIAASTLDSLLDFLAGGILYFTHLTMKSVNIYK 180

Query: 181 FPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISAT 240
           +PIGKLRVQPVGIIVFAA+MATLGFQVL+QA EQL+++K  E ++PEQ IWL +IM+SAT
Sbjct: 181 YPIGKLRVQPVGIIVFAAIMATLGFQVLIQAIEQLVENKAGEKMTPEQLIWLYSIMLSAT 240

Query: 241 VVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIY 300
           VVKL L++YC+SS N IV+AYAKDHYFDVVTNVVGLVAA+LGDK FWWIDP+GA+ LA+Y
Sbjct: 241 VVKLALYIYCRSSGNSIVQAYAKDHYFDVVTNVVGLVAAVLGDKFFWWIDPVGAVLLAVY 300

Query: 301 TILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRH-PEVKRVDTVRAYTFGVLYFVEVD 360
           TI+NWSGTV+ENAV+LVG+ AP ++LQKLTYL  +H P V+RVDTVRAY+FG LYFVEVD
Sbjct: 301 TIVNWSGTVYENAVTLVGQCAPSDMLQKLTYLAMKHDPRVRRVDTVRAYSFGALYFVEVD 360

Query: 361 IELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 408
           IEL E++ L EAH+IGE+LQ KIEKLPEVERAFVH+DFE  HKPEH +  RLP+T
Sbjct: 361 IELSEDMRLGEAHSIGESLQDKIEKLPEVERAFVHVDFESTHKPEHRVRSRLPST 408

BLAST of Csor.00g225360 vs. ExPASy Swiss-Prot
Match: Q5NA18 (Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.2e-97
Identity = 204/399 (51.13%), Postives = 269/399 (67.42%), Query Frame = 0

Query: 13  LLAGAVKRD--GSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTA 72
           LL  AV+    G G  G  +   +  S R  F    P +V+   Q+   P  +       
Sbjct: 16  LLLSAVEAGSFGGGGDGGGAGAAAEKSWRLNFDGFRPPEVQ---QERRPPRGLHHHCLGV 75

Query: 73  FSRGEKD----YYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQ----EERAMKISNYA 132
            S+G +D    YY++Q+  L+ F E+D+L     +     EER +    E  A+++SN A
Sbjct: 76  LSQGPEDVVAEYYQQQVEMLEGFNEMDTLTDRGFLPGMSKEEREKVARSETLAIRLSNIA 135

Query: 133 NIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQ 192
           N+VL   K+YA+VRSGS+AI ASTLDSLLDLL+G ILWFT   M+  N Y++PIGK R+Q
Sbjct: 136 NMVLFAAKVYASVRSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYRYPIGKKRMQ 195

Query: 193 PVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLY 252
           P+GI+VFA+VMATLG Q++L++   L+ D     L+ EQ  W+  IM++ T+VKL L LY
Sbjct: 196 PLGILVFASVMATLGLQIILESVRSLLSDGDEFSLTKEQEKWVVDIMLAVTLVKLALVLY 255

Query: 253 CKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTV 312
           C++  N+IV+AYA+DH+FDV+TN++GLVAA+L   I  WIDP+GAI LAIYTI  WS TV
Sbjct: 256 CRTFTNEIVKAYAQDHFFDVITNMIGLVAALLATYIEGWIDPVGAIILAIYTIRTWSMTV 315

Query: 313 WENAVSLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPL 372
            EN  SLVG+SA PE LQKLTYL    H  V+ +DTVRAYTFG  YFVEVDI LP  +PL
Sbjct: 316 LENVHSLVGQSASPEYLQKLTYLCWNHHKAVRHIDTVRAYTFGSHYFVEVDIVLPSSMPL 375

Query: 373 KEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSI 401
           +EAH IGE LQ K+E+LPE+ERAFVHLD+E  H+PEH++
Sbjct: 376 QEAHDIGEALQEKLERLPEIERAFVHLDYEFTHRPEHAL 411

BLAST of Csor.00g225360 vs. ExPASy Swiss-Prot
Match: O80632 (Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.1e-95
Identity = 183/328 (55.79%), Postives = 240/328 (73.17%), Query Frame = 0

Query: 77  DYYERQIATLKSFEEVDSLVTSDCIDEEDSEER----AQEERAMKISNYANIVLLLLKIY 136
           DYY++Q+  L+ F E+D L     +     EE+      E  A++ISN AN++L   K+Y
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAKVY 122

Query: 137 ATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAV 196
           A+V SGS+AI ASTLDSLLDLL+G ILWFT   M+  N Y++PIGK R+QP+GI+VFA+V
Sbjct: 123 ASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASV 182

Query: 197 MATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVR 256
           MATLG Q++L++   ++       L+ EQ  W+  IM+S T+VKL+L LYC+S  N+IV+
Sbjct: 183 MATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVLYCRSFTNEIVK 242

Query: 257 AYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGK 316
           AYA+DH+FDV+TN++GL+A IL + I +WIDP+GAI LA+YTI  WS TV EN  SLVGK
Sbjct: 243 AYAQDHFFDVITNIIGLIAVILANYIDYWIDPVGAIILALYTIRTWSMTVLENVNSLVGK 302

Query: 317 SAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETL 376
           SA PE LQKLTYL    H  ++ +DTVRAYTFG  YFVEVDI LP ++PL+ AH IGE+L
Sbjct: 303 SARPEYLQKLTYLCWNHHKAIRHIDTVRAYTFGSHYFVEVDIVLPADMPLQVAHDIGESL 362

Query: 377 QIKIEKLPEVERAFVHLDFECEHKPEHS 400
           Q K+E L E+ERAFVHLD+E  HKPEH+
Sbjct: 363 QEKLELLEEIERAFVHLDYEYTHKPEHA 390

BLAST of Csor.00g225360 vs. NCBI nr
Match: KAG6590238.1 (Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 788 bits (2036), Expect = 2.63e-287
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. NCBI nr
Match: XP_022960581.1 (metal tolerance protein 4 [Cucurbita moschata])

HSP 1 Score: 787 bits (2033), Expect = 7.53e-287
Identity = 406/407 (99.75%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQI+TLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQISTLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. NCBI nr
Match: XP_023516649.1 (metal tolerance protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 784 bits (2024), Expect = 1.77e-285
Identity = 404/407 (99.26%), Postives = 405/407 (99.51%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
            YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 FYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. NCBI nr
Match: KAG7023879.1 (Metal tolerance protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780 bits (2014), Expect = 8.97e-284
Identity = 407/418 (97.37%), Postives = 407/418 (97.37%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418

BLAST of Csor.00g225360 vs. NCBI nr
Match: XP_022988141.1 (metal tolerance protein 4 [Cucurbita maxima])

HSP 1 Score: 779 bits (2011), Expect = 1.70e-283
Identity = 402/407 (98.77%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG PD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
            YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 FYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLV RHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVIRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. ExPASy TrEMBL
Match: A0A6J1H803 (metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=1)

HSP 1 Score: 787 bits (2033), Expect = 3.65e-287
Identity = 406/407 (99.75%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQI+TLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQISTLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. ExPASy TrEMBL
Match: A0A6J1JGE1 (metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1)

HSP 1 Score: 779 bits (2011), Expect = 8.22e-284
Identity = 402/407 (98.77%), Postives = 403/407 (99.02%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG PD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
            YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG
Sbjct: 241 FYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQKLTYLV RHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQKLTYLVIRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Csor.00g225360 vs. ExPASy TrEMBL
Match: A0A5A7THE5 (Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00130 PE=4 SV=1)

HSP 1 Score: 724 bits (1868), Expect = 5.27e-262
Identity = 372/407 (91.40%), Postives = 387/407 (95.09%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM  ATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIALAIYTILNWSG
Sbjct: 241 LYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL RLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSRLPNT 406

BLAST of Csor.00g225360 vs. ExPASy TrEMBL
Match: A0A1S3BQ61 (metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 724 bits (1868), Expect = 5.27e-262
Identity = 372/407 (91.40%), Postives = 387/407 (95.09%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM  ATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIALAIYTILNWSG
Sbjct: 241 LYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL RLPNT
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSRLPNT 406

BLAST of Csor.00g225360 vs. ExPASy TrEMBL
Match: A0A1S3BRU5 (metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 715 bits (1845), Expect = 1.74e-258
Identity = 368/403 (91.32%), Postives = 383/403 (95.04%), Query Frame = 0

Query: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIYKFPIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM  ATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSG 300
           LYCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIALAIYTILNWSG
Sbjct: 241 LYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIALAIYTILNWSG 300

Query: 301 TVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360
           TVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP
Sbjct: 301 TVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 360

Query: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRR 403
           LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL R
Sbjct: 361 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSR 402

BLAST of Csor.00g225360 vs. TAIR 10
Match: AT3G58060.1 (Cation efflux family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.2e-150
Identity = 277/381 (72.70%), Postives = 324/381 (85.04%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDS 94
           V+S++S F + LP+K++S + D E+P  +D+S++      EK+YYERQ+ATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  -LVTSD--CID----EEDSEERAQEERAMKISNYANIVLLLLKIYATVRSGSIAIAASTL 154
            L  SD   ID    EED  ERA +E AM+ISN+ANI LL LKIYATV+SGSIAIAASTL
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALKIYATVKSGSIAIAASTL 149

Query: 155 DSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQ 214
           DSLLDL+AGGILWFTHL MK VNIYK+PIGKLRVQPVGII+FAAVMATLGFQVLL AAEQ
Sbjct: 150 DSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATLGFQVLLVAAEQ 209

Query: 215 LIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVV 274
           LI ++PSE ++  Q IWL +IM+SAT +KLVLW+YCKSSRN IVRAYAKDH+FDVVTNV+
Sbjct: 210 LISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAKDHHFDVVTNVL 269

Query: 275 GLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVT 334
           GLVAA+L +  +WW+DP GAI LAIYTI+NWSGTV ENAVSL+G+SAPPEVLQKLTYLV 
Sbjct: 270 GLVAAVLANAFYWWLDPTGAILLAIYTIVNWSGTVMENAVSLIGQSAPPEVLQKLTYLVM 329

Query: 335 RH--PEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAF 394
           R     +K VDTVRAYTFGVLYFVEVDIELPE+LPLKEAHAIGE+LQIK+E+LPEVERAF
Sbjct: 330 RQGGDNIKHVDTVRAYTFGVLYFVEVDIELPEDLPLKEAHAIGESLQIKLEELPEVERAF 389

Query: 395 VHLDFECEHKPEHSILRRLPN 407
           VHLDFEC HKPEHS+L  +PN
Sbjct: 390 VHLDFECHHKPEHSVLSTIPN 409

BLAST of Csor.00g225360 vs. TAIR 10
Match: AT2G39450.1 (Cation efflux family protein )

HSP 1 Score: 351.7 bits (901), Expect = 8.1e-97
Identity = 183/328 (55.79%), Postives = 240/328 (73.17%), Query Frame = 0

Query: 77  DYYERQIATLKSFEEVDSLVTSDCIDEEDSEER----AQEERAMKISNYANIVLLLLKIY 136
           DYY++Q+  L+ F E+D L     +     EE+      E  A++ISN AN++L   K+Y
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAKVY 122

Query: 137 ATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAV 196
           A+V SGS+AI ASTLDSLLDLL+G ILWFT   M+  N Y++PIGK R+QP+GI+VFA+V
Sbjct: 123 ASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASV 182

Query: 197 MATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSRNDIVR 256
           MATLG Q++L++   ++       L+ EQ  W+  IM+S T+VKL+L LYC+S  N+IV+
Sbjct: 183 MATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVLYCRSFTNEIVK 242

Query: 257 AYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGK 316
           AYA+DH+FDV+TN++GL+A IL + I +WIDP+GAI LA+YTI  WS TV EN  SLVGK
Sbjct: 243 AYAQDHFFDVITNIIGLIAVILANYIDYWIDPVGAIILALYTIRTWSMTVLENVNSLVGK 302

Query: 317 SAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETL 376
           SA PE LQKLTYL    H  ++ +DTVRAYTFG  YFVEVDI LP ++PL+ AH IGE+L
Sbjct: 303 SARPEYLQKLTYLCWNHHKAIRHIDTVRAYTFGSHYFVEVDIVLPADMPLQVAHDIGESL 362

Query: 377 QIKIEKLPEVERAFVHLDFECEHKPEHS 400
           Q K+E L E+ERAFVHLD+E  HKPEH+
Sbjct: 363 QEKLELLEEIERAFVHLDYEYTHKPEHA 390

BLAST of Csor.00g225360 vs. TAIR 10
Match: AT1G79520.1 (Cation efflux family protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.4e-95
Identity = 187/332 (56.33%), Postives = 243/332 (73.19%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 68  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 127

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGII 192
           + K+YA+V S S+A+ ASTLDSLLDLL+G ILWFT   M+  N +++PIGK R+QPVGII
Sbjct: 128 VAKVYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPIGKRRMQPVGII 187

Query: 193 VFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSR 252
           VFA+VMATLG QV+L++   L+    S   S E+  W+  IM SATVVK +L LYC+S +
Sbjct: 188 VFASVMATLGLQVILESTRLLVSKNGSHMSSTEE-KWMIGIMASATVVKFLLMLYCRSFQ 247

Query: 253 NDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAV 312
           N+IVRAYA+DH FDV+TN VGL  A+L  K +WWIDP GAI +A+YTI  W+ TV EN  
Sbjct: 248 NEIVRAYAQDHLFDVITNSVGLATAVLAVKFYWWIDPSGAILIALYTISTWARTVLENVH 307

Query: 313 SLVGKSAPPEVLQKLTYLVTRHPE-VKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHA 372
           SL+G+SAPP+ L KLT+L+  H E +K +DTVRAYTFG  YFVEVDI LPE++ L EAH 
Sbjct: 308 SLIGRSAPPDFLAKLTFLIWNHHEKIKHIDTVRAYTFGSHYFVEVDIVLPEDMRLHEAHN 367

Query: 373 IGETLQIKIEKLPEVERAFVHLDFECEHKPEH 399
           IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 368 IGETLQEKLEQLSEVERAFVHIDFEFTHRPEH 398

BLAST of Csor.00g225360 vs. TAIR 10
Match: AT1G79520.2 (Cation efflux family protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.4e-95
Identity = 187/332 (56.33%), Postives = 243/332 (73.19%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 80  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 139

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGII 192
           + K+YA+V S S+A+ ASTLDSLLDLL+G ILWFT   M+  N +++PIGK R+QPVGII
Sbjct: 140 VAKVYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPIGKRRMQPVGII 199

Query: 193 VFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSR 252
           VFA+VMATLG QV+L++   L+    S   S E+  W+  IM SATVVK +L LYC+S +
Sbjct: 200 VFASVMATLGLQVILESTRLLVSKNGSHMSSTEE-KWMIGIMASATVVKFLLMLYCRSFQ 259

Query: 253 NDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAV 312
           N+IVRAYA+DH FDV+TN VGL  A+L  K +WWIDP GAI +A+YTI  W+ TV EN  
Sbjct: 260 NEIVRAYAQDHLFDVITNSVGLATAVLAVKFYWWIDPSGAILIALYTISTWARTVLENVH 319

Query: 313 SLVGKSAPPEVLQKLTYLVTRHPE-VKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHA 372
           SL+G+SAPP+ L KLT+L+  H E +K +DTVRAYTFG  YFVEVDI LPE++ L EAH 
Sbjct: 320 SLIGRSAPPDFLAKLTFLIWNHHEKIKHIDTVRAYTFGSHYFVEVDIVLPEDMRLHEAHN 379

Query: 373 IGETLQIKIEKLPEVERAFVHLDFECEHKPEH 399
           IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 380 IGETLQEKLEQLSEVERAFVHIDFEFTHRPEH 410

BLAST of Csor.00g225360 vs. TAIR 10
Match: AT1G16310.1 (Cation efflux family protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.2e-94
Identity = 181/332 (54.52%), Postives = 243/332 (73.19%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN  N+VL 
Sbjct: 95  RRVSEYYKKQERLLEGFNEMETIHENGFASGVPTEEEMKKLAKSERLAVHISNATNLVLF 154

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGII 192
           + K+YA++ S S+A+ ASTLDSLLDLL+G ILWFT   M++ N + +PIGK R+QPVGII
Sbjct: 155 VAKVYASMESRSMAVIASTLDSLLDLLSGFILWFTANAMRKPNQFHYPIGKRRMQPVGII 214

Query: 193 VFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLWLYCKSSR 252
           VFA+VMATLG QVLL++  QL+  K    ++  +  W+  IM+S T+VK +L LYC+  +
Sbjct: 215 VFASVMATLGLQVLLESGRQLVA-KSGIHMNSTEEKWMIGIMVSVTIVKFLLMLYCRGFQ 274

Query: 253 NDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAV 312
           N+IVRAYA+DH FDVVTN +GL  A+L  K +WWIDP GAI +A+YTI  W+ TV EN  
Sbjct: 275 NEIVRAYAQDHLFDVVTNSIGLATAVLAVKFYWWIDPTGAILIALYTIATWARTVLENVH 334

Query: 313 SLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHA 372
           SL+G+SAPP+ L KLT+L+   H ++K +DTVRAYTFG  YFVEVDI LPE++ L+EAH 
Sbjct: 335 SLIGRSAPPDFLAKLTFLIWNHHEQIKHIDTVRAYTFGSHYFVEVDIVLPEDMRLQEAHN 394

Query: 373 IGETLQIKIEKLPEVERAFVHLDFECEHKPEH 399
           IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 395 IGETLQEKLEQLAEVERAFVHIDFEFTHRPEH 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10PP84.8e-15573.61Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 ... [more]
Q9M2P23.0e-14972.70Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=... [more]
Q6Z7K55.0e-14464.82Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 ... [more]
Q5NA181.2e-9751.13Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 ... [more]
O806321.1e-9555.79Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6590238.12.63e-287100.00Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960581.17.53e-28799.75metal tolerance protein 4 [Cucurbita moschata][more]
XP_023516649.11.77e-28599.26metal tolerance protein 4 [Cucurbita pepo subsp. pepo][more]
KAG7023879.18.97e-28497.37Metal tolerance protein 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022988141.11.70e-28398.77metal tolerance protein 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1H8033.65e-28799.75metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=... [more]
A0A6J1JGE18.22e-28498.77metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1[more]
A0A5A7THE55.27e-26291.40Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ615.27e-26291.40metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
A0A1S3BRU51.74e-25891.32metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
Match NameE-valueIdentityDescription
AT3G58060.12.2e-15072.70Cation efflux family protein [more]
AT2G39450.18.1e-9755.79Cation efflux family protein [more]
AT1G79520.13.4e-9556.33Cation efflux family protein [more]
AT1G79520.23.4e-9556.33Cation efflux family protein [more]
AT1G16310.12.2e-9454.52Cation efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036837Cation efflux protein, cytoplasmic domain superfamilyGENE3D3.30.70.1350Cation efflux protein, cytoplasmic domaincoord: 312..390
e-value: 2.4E-17
score: 64.9
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 313..391
IPR027470Cation efflux protein, cytoplasmic domainPFAMPF16916ZT_dimercoord: 315..389
e-value: 4.0E-13
score: 49.3
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 113..389
e-value: 5.2E-35
score: 119.0
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 118..310
e-value: 1.8E-26
score: 93.2
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 112..309
e-value: 1.6E-38
score: 134.3
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 111..311
NoneNo IPR availablePANTHERPTHR43840:SF29METAL TOLERANCE PROTEIN 3coord: 16..405
NoneNo IPR availablePANTHERPTHR43840MITOCHONDRIAL METAL TRANSPORTER 1-RELATEDcoord: 16..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g225360.m01Csor.00g225360.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0008324 cation transmembrane transporter activity