Csor.00g208380 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g208380
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionTerpene cyclase/mutase family member
LocationCsor_Chr05: 7813731 .. 7816673 (+)
RNA-Seq ExpressionCsor.00g208380
SyntenyCsor.00g208380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGACTTACGGTGGGAGAAGGAGCAGATGATCCGTACTTGTTCAGCTCCAACAACTTTGTGGGGAGACAAACGTGGGACTTCGAGCCCGATGATGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCTCGTCAAAATTATTACCATAATCGTTTCAAAGTCCCATGCAACAGTGATCTCTTTTGGAGATTTCAGGTCCTCTCTTTATATATTATAAAAGTTTTTTATTTTCTAAAATCAAATATAGATTTATAGGTTTAAAAATGATTAAATCTAACGTTAGTATGAGTTTAAAATGATCTTTACATGCATGGAATGTGTCTCATTTAAAATGATCTTAGAAGGGACATGATTCTTTACGAAGAACAGAACTTAGTAACCCTTCTTCTTCAGCTTCTTATTTTACCATATATGTTTGAACTTATAAGATTCCATTTCATAATAAAATTCAATTTTTTTTTTCTCAATTCAAAATTTTGCTAAGATTTCAAGTGACCCATTTCGAGTCGTCGAACAACCTCCCCTTAATTAAGGTCTGGGGATTCACTCTCAACAATCATCAATAATCCTCCTCTCGAACAAAGTACACCATAGAGCTCTCAAACAGCCTCCCCTTAATCGAGGCTCGACTCCTTGTCTGGAGTCCTCGAACAATGTACACCCTTTTTGAGCTTAAGCTCTCATGATATGATATTCCTTACGTATAAACTCATGATCATTCCCTAAATTAGCTAATGTGAGACTCACTCCCAACCATCCTCAACAATTGTTAGTAGTTTACTTAAAAAAATATCCTTGAAATCTTGATAGTGTTCAAAAAATACCCTTAAACTTTAAAAACTTTCGTTAAAAACCTTCAAATTTTTAAAAAAGTTAAAAAAAAAAAACCTTCTTCGTTAGTACTAACACCCTTTAACTTTATAAAATAATAATAATAATAAATCTATCGAACTCCTCATTGATTATAACTTTTTAATTTTAATTTTAAAAAAGTTCATAAATATTTTTGAACCATCATATTTTTTACTTTCAAACAAATTTAAATGGTGTTTTCAAAGTTTTTAAAACAGTTTAGTTTAAACGTTTAAATTTTTAAAAAAGTTGAAAGATATTAATAAAACTTTTGAAAATTTAAAAATATTTTTTAATGGCTTTCCATTTTAAAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTTTTAAGAGTATTATTAAGAAGTTTAAAGATATTATTTAAGTTTTTGAAAATTTTTATGTAAGGATATTTTTATTAATTAGTAGATATTTTTATTAATGAGTGAAGGTAAAATGGAGGATTTACTTCATATTTTGAATTCAAAACTTTATGAGTAAATTCAGTCTACGTCATCATTAAATGTAAAATTTGGAAGGAAGTAGACATATATTTGATTGGTCTACTTTATCAATAAATGTGTATTTAAATAAGTGTTTGAAGGGACATACTTCTTTTGTGAGGATCAGTTTCTGAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAGAGTGGAGGATGTAATAGATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAACTTCTTTGCAGCCTTACAAAGCCCCCACGGCCATTGGCCTGCTGAAAACGCTGGCCCTATGTTTTATTTCCCTCCACTGGTACTCTTCCTTTTTCTATTCATTTTATCTTATATACTTCTACCACAATATTACCCTTTTCTTTTCAGGTATTTTCGCTGTATATTACGGGACATCTCCCCATCATATTCTCAGAACAGCACCGCAAGGAAATCCTTCGTTACTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGTGGGACAAAGTTGTATGTTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGAGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTCGATCATGGAGGTGCTCTTTACATACCCTCTTGGGGAAAGATTTGGCTCTCGGTTAGTACTATATATATACGTCTATACTATTTCATTTGAAACGACATAACTCAAGTTTAAGGAACTAAACTTATAATGTAACTATGTAGATTCTTGGAGTTTACGAGTGGGAGGGAACAAATCCTATGCCTCCAGAGTTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCATATTTATATGGGAAGCGATTTGTTGGACCCCTCACACCTCTCATTCTTCAATTACGTCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACTCGTCATTATTGTGCAAAGGTACGCTCCTAAACCATTCAACCCTCTCTACAACAACAATGCACGAGTAAAGAACAAATCAATATTCAACCTTTCAAAAGGTTTGAGATTTGTACGGTATGAGACTTTGATTACTTTGATTGAATTGGACATGATGATGTAGGAAGACAAATGCTTTGAACGTTCTTTATTTCAGAAGCTTGCATGGGATGCTCTTCAATACTTTGGAGAACCCATTCTTAATAGTTGGGCTTTTAAAACAATAAGAAATAGAGCCCTTCAAATAGCTAAACGTCATATTGATTATGAAGATCATAACAGTCATTACATTACAATTGGATGCATTGAAAAGGTTAATTTCATCCTTCTTTTTTCTTTTAGGTCATAATTATAGATAATAATATTATTATTAATAAATATAATTGAGCTAATATGGTGGAGGGGTATTGAACATGCAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTATGTTTTACCCATCATTAA

mRNA sequence

ATGTGGAGACTTACGGTGGGAGAAGGAGCAGATGATCCGTACTTGTTCAGCTCCAACAACTTTGTGGGGAGACAAACGTGGGACTTCGAGCCCGATGATGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCTCGTCAAAATTATTACCATAATCGTTTCAAAGTCCCATGCAACAGTGATCTCTTTTGGAGATTTCAGTTTCTGAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAGAGTGGAGGATGTAATAGATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAACTTCTTTGCAGCCTTACAAAGCCCCCACGGCCATTGGCCTGCTGAAAACGCTGGCCCTATGTTTTATTTCCCTCCACTGAACAGCACCGCAAGGAAATCCTTCGTTACTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGTGGGACAAAGTTGTATGTTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGAGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTCGATCATGGAGATTCTTGGAGTTTACGAGTGGGAGGGAACAAATCCTATGCCTCCAGAGTTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCATATTTATATGGGAAGCGATTTGTTGGACCCCTCACACCTCTCATTCTTCAATTACGTCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACTCGTCATTATTGTGCAAAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTATGTTTTACCCATCATTAA

Coding sequence (CDS)

ATGTGGAGACTTACGGTGGGAGAAGGAGCAGATGATCCGTACTTGTTCAGCTCCAACAACTTTGTGGGGAGACAAACGTGGGACTTCGAGCCCGATGATGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCTCGTCAAAATTATTACCATAATCGTTTCAAAGTCCCATGCAACAGTGATCTCTTTTGGAGATTTCAGTTTCTGAGAGAGCGAAATTTCAAGCAAACAATTCCAATAGTGAGAGTGGAGGATGTAATAGATAAGGAAACAGCGAGCATTGCACTGAGAAGAGCCACCAACTTCTTTGCAGCCTTACAAAGCCCCCACGGCCATTGGCCTGCTGAAAACGCTGGCCCTATGTTTTATTTCCCTCCACTGAACAGCACCGCAAGGAAATCCTTCGTTACTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGTGGGACAAAGTTGTATGTTTTGCACTGTTTTTAACTATATTCAACTCCGTTTGTTGGGAGAAGAACCTGACAAGGATGAGTGTTTTAGAGCTCGAAAATGGATTCTCGATCATGGAGATTCTTGGAGTTTACGAGTGGGAGGGAACAAATCCTATGCCTCCAGAGTTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCATATTTATATGGGAAGCGATTTGTTGGACCCCTCACACCTCTCATTCTTCAATTACGTCAAGAAATCTATACTCAACCTTACAATCACATTAAATGGAGTCCAACTCGTCATTATTGTGCAAAGCCATTGTTCACACTTGCTTGTTGGGTCGATGATCCTAATGGGGAAGCTTACAAGAAACATGTTGCTAGAATCAAAGATTACTTATGGATTGCTGAAGATGGAATGAAGATGCAAGTATGTTTTACCCATCATTAA

Protein sequence

MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNSDLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGPMFYFPPLNSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIFNSVCWEKNLTRMSVLELENGFSIMEILGVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPTRHYCAKPLFTLACWVDDPNGEAYKKHVARIKDYLWIAEDGMKMQVCFTHH
Homology
BLAST of Csor.00g208380 vs. ExPASy Swiss-Prot
Match: E7DN63 (Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.2e-89
Identity = 176/410 (42.93%), Postives = 231/410 (56.34%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MW+L + EG + PYL+S+NN+VGRQTW+F+P+ GT EERA++EEARQ +++NR+KV  +S
Sbjct: 1   MWKLKIAEGQNGPYLYSTNNYVGRQTWEFDPNGGTIEERAKIEEARQQFWNNRYKVKPSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVE--DVIDKETASIALRRATNFFAALQSPHGHWPAENA 120
           DL WR QFL E+NFKQ IP V+VE  + I  E A+IAL RA NFF+ALQ+  GHWPAENA
Sbjct: 61  DLLWRIQFLGEKNFKQKIPAVKVEEGEEISHEVATIALHRAVNFFSALQATDGHWPAENA 120

Query: 121 GPMFYFPPL--------------NSTARKSFVTHIVIRMK-------MVDGDCTLWDKVV 180
           GP+F+ PPL               +  RK  + +I             ++G  T++   +
Sbjct: 121 GPLFFLPPLVMCMYITGHLNTVFPAEHRKEILRYIYCHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 181 CFALFLTI-------FNSVCWEKN---LTRMSVLELEN-GFSIMEILGVYEWEGTNPMPP 240
            +     +        N+ C       L   SV  + + G + + ILGV+EW GTNPMPP
Sbjct: 181 SYICMRILGEGPDGGVNNACARARKWILDHGSVTAIPSWGKTWLSILGVFEWIGTNPMPP 240

Query: 241 EFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKW 300
           EFW+L   LP  P  ++CY R+  +PMSYLYGKRFVGP+TPLILQLR+E+Y +PY+ I W
Sbjct: 241 EFWILPSFLPVHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYDRPYDEINW 300

Query: 301 SPTRHYCAK-----------------------PLFT------------------------ 314
              RH CAK                       PL T                        
Sbjct: 301 KKVRHVCAKEDLYYPHPLVQDLMWDSLYICTEPLLTRWPFNKLRNKALEVTMKHIHYEDE 360

BLAST of Csor.00g208380 vs. ExPASy Swiss-Prot
Match: Q8W3Z1 (Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.3e-88
Identity = 170/411 (41.36%), Postives = 232/411 (56.45%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL + +G  DPY++S+NNFVGRQTW+F+P  G+P+ERAEVEEAR+N+Y NR++V  + 
Sbjct: 1   MWRLKIADGGSDPYIYSTNNFVGRQTWEFDPQAGSPQERAEVEEARRNFYDNRYQVKPSG 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVED--VIDKETASIALRRATNFFAALQSPHGHWPAENA 120
           DL WR QFL+E+NFKQTIP V+VED   I  E ++ ALRRA +F++ALQ+  GHWPAENA
Sbjct: 61  DLLWRMQFLKEKNFKQTIPPVKVEDGEEITYEKSTAALRRAVHFYSALQASDGHWPAENA 120

Query: 121 GPMFYFPPL--------------NSTARKSFVTHIVIRMK-------MVDGDCTLWDKVV 180
           GP+F+ PPL               +  +K  + +I             ++G  T++   +
Sbjct: 121 GPLFFLPPLVMCMYITGHLNTVFPAEHQKEILRYIYYHQNEDGGWGLHIEGHSTMFCTAL 180

Query: 181 CFALFLTI-------FNSVCWEKN---LTRMSVLELEN-GFSIMEILGVYEWEGTNPMPP 240
            +     +        ++ C       L    V  + + G + + ILG++EW G+NPMPP
Sbjct: 181 SYICMRILGEGPDGGQDNACARARKWILDHGGVTHMPSWGKTWLSILGIFEWIGSNPMPP 240

Query: 241 EFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKW 300
           EFW+L   LP  P  ++CY R+  +PMSYLYGKRFVGP+TPLILQLR+E+YTQPY+ + W
Sbjct: 241 EFWILPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYTQPYHQVNW 300

Query: 301 SPTRHYCAK-----------------------PLFT------------------------ 314
              RH CAK                       PL T                        
Sbjct: 301 KKVRHLCAKEDIYYPHPLIQDLLWDSLYIFTEPLLTRWPFNKLVREKALQVTMKHIHYED 360

BLAST of Csor.00g208380 vs. ExPASy Swiss-Prot
Match: A8C980 (Germanicol synthase OS=Rhizophora stylosa OX=98588 GN=M1 PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.3e-88
Identity = 178/415 (42.89%), Postives = 228/415 (54.94%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL + EG +DPYL+S+NN+VGRQ W+F+PD GTPEERA+ EEARQN+Y NR++V  + 
Sbjct: 1   MWRLKIAEGGNDPYLYSTNNYVGRQIWEFDPDAGTPEERAKAEEARQNFYKNRYQVKPSG 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVE--DVIDKETASIALRRATNFFAALQSPHGHWPAENA 120
           DL WR QFLRE+NFKQTIP VR+E  + I +E A+ ALRRA  FF+ALQ+  GHWPAENA
Sbjct: 61  DLLWRLQFLREKNFKQTIPQVRIEEGEEITREKATTALRRAVQFFSALQASDGHWPAENA 120

Query: 121 GPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVV--CFALF 180
           GP+F+ PPL               +  RK  + +I        G    W   +     +F
Sbjct: 121 GPLFFLPPLVMCMCITGHLDTVFPAEHRKEILRYIYYHQNEDGG----WGLHIEGHSTMF 180

Query: 181 LTIFNSVCW-----------EKNLTRM--------SVLELEN-GFSIMEILGVYEWEGTN 240
            T  N +C            +   TR         SV  + + G + + ILGVY+W G N
Sbjct: 181 CTALNYICMRILGEGPNGGQDDACTRARKWIHDHGSVTNIPSWGKTWLSILGVYDWSGCN 240

Query: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFW+L   LP  P  ++CY R+  +PMSYLYGKRFVG +TPLI QLR+E++TQPY+
Sbjct: 241 PMPPEFWMLPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGLITPLIQQLREELFTQPYD 300

Query: 301 HIKWSPTRHYCA-----------------------KPLFT-------------------- 314
            I W    H CA                       +PL T                    
Sbjct: 301 QINWKKNCHQCAPEDLYYPHPFIQDLIWDCLYISMEPLLTRWPLNMIIRKKALELTMKHI 360

BLAST of Csor.00g208380 vs. ExPASy Swiss-Prot
Match: A8CDT2 (Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.8e-88
Identity = 178/417 (42.69%), Postives = 227/417 (54.44%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWR+ + EG  DPYL+S+NN+VGRQTW+F+PD GTPEERAEVEEARQN+Y NR++V    
Sbjct: 1   MWRIKIAEGGKDPYLYSTNNYVGRQTWEFDPDAGTPEERAEVEEARQNFYKNRYQVKPCG 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVE--DVIDKETASIALRRATNFFAALQSPHGHWPAENA 120
           DL WR QFL E+NF+QTIP VR+E  + I  E A+ ALRR   FF+ALQ+  GHWPAE A
Sbjct: 61  DLLWRLQFLGEKNFEQTIPQVRIEEGEGITYEKATRALRRTVQFFSALQASDGHWPAEIA 120

Query: 121 GPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVV--CFALF 180
           GP+F+ PPL               +  RK  + +I        G    W   +     +F
Sbjct: 121 GPLFFLPPLVMCVYITGHLDAVFPAEHRKEILRYIYYHQNEDGG----WGLHIEGHSTMF 180

Query: 181 LTIFNSVC---------------------WEKNLTRMSVLELEN-GFSIMEILGVYEWEG 240
            T  N +C                     W  +    SV  + + G + + ILGVY+W G
Sbjct: 181 CTALNYICMRIIGEGPNGGQDDACARARKWIHD--HGSVTNIPSWGKTWLSILGVYDWSG 240

Query: 241 TNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQP 300
           +NPMPPEFW+L   LP  P  ++CY R+  +PMSYLYGKRFVGP+TPLI QLR+E++TQP
Sbjct: 241 SNPMPPEFWMLPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLIQQLREELFTQP 300

Query: 301 YNHIKWSPTRHYCA-----------------------KPLFT------------------ 314
           Y+ I W  TRH CA                       +PL T                  
Sbjct: 301 YDQINWKKTRHQCAPEDLYYPHPFVQDLIWDCLYIFTEPLLTRWPLNEIIRKKALEVTMK 360

BLAST of Csor.00g208380 vs. ExPASy Swiss-Prot
Match: Q2XPU7 (Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-87
Identity = 174/414 (42.03%), Postives = 225/414 (54.35%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWR+ + EG ++PY++S+NNF GRQ W F+P+ GTPEE+AEVEEARQN++ NRF+V  NS
Sbjct: 1   MWRIKIAEGGNNPYIYSTNNFQGRQIWVFDPNAGTPEEQAEVEEARQNFWKNRFQVKPNS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVED--VIDKETASIALRRATNFFAALQSPHGHWPAENA 120
           DL W+ QFLRE+NFKQ IP V+VED   I  E A+ ALRR+ + F+ALQ+  GHW AEN 
Sbjct: 61  DLLWQLQFLREKNFKQKIPKVKVEDGEEITSEIAAAALRRSVHLFSALQASDGHWCAENG 120

Query: 121 GPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVV--CFALF 180
           G +F+ PPL              +   RK  + +I        G    W   +     +F
Sbjct: 121 GLLFFLPPLVFAVYITGHLNTVFSPEHRKEILRYIYCHQNEDGG----WGIHIEGHSTMF 180

Query: 181 LTIFNSVCW-----------EKNLTRMSVLELENGFSI---------MEILGVYEWEGTN 240
            T+ N +C            E    R     L++G +          + ILGVYEW+GTN
Sbjct: 181 CTVLNYICMRILGEARDGGIENACERGRKWILDHGGATGISSWGKTWLSILGVYEWDGTN 240

Query: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYN 300
           PMPPEFW      P  P  +FCY R+T +PMSYLYGKRFVGP+TPLILQ+R+EIY +PYN
Sbjct: 241 PMPPEFWAFPSSFPLHPAKMFCYCRITYMPMSYLYGKRFVGPITPLILQIREEIYNEPYN 300

Query: 301 HIKWSPTRHYCA------------------------------------------------ 314
            IKW+  RH CA                                                
Sbjct: 301 KIKWNSVRHLCAKEDNYFPHPTIQKLLWDALYTFSEPLFSRWPFNKLREKALKITMDHIH 360

BLAST of Csor.00g208380 vs. NCBI nr
Match: KAG6599056.1 (Beta-amyrin synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 685 bits (1768), Expect = 1.92e-249
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120
           DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120

Query: 121 MFYFPPLNSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIFNSVCWEKNLTRMSV 180
           MFYFPPLNSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIFNSVCWEKNLTRMSV
Sbjct: 121 MFYFPPLNSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIFNSVCWEKNLTRMSV 180

Query: 181 LELENGFSIMEILGVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKR 240
           LELENGFSIMEILGVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKR
Sbjct: 181 LELENGFSIMEILGVYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKR 240

Query: 241 FVGPLTPLILQLRQEIYTQPYNHIKWSPTRHYCAKPLFTLACWVDDPNGEAYKKHVARIK 300
           FVGPLTPLILQLRQEIYTQPYNHIKWSPTRHYCAKPLFTLACWVDDPNGEAYKKHVARIK
Sbjct: 241 FVGPLTPLILQLRQEIYTQPYNHIKWSPTRHYCAKPLFTLACWVDDPNGEAYKKHVARIK 300

Query: 301 DYLWIAEDGMKMQVCFTHH 319
           DYLWIAEDGMKMQVCFTHH
Sbjct: 301 DYLWIAEDGMKMQVCFTHH 319

BLAST of Csor.00g208380 vs. NCBI nr
Match: KAG6599032.1 (hypothetical protein SDJN03_08810, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 492 bits (1266), Expect = 1.31e-171
Identity = 263/407 (64.62%), Postives = 273/407 (67.08%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120
           DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120

Query: 121 MFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIF 180
           MFYFPPL              +   RK  + +        DG   L   V    +F T+F
Sbjct: 121 MFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNE-DGGWGL-HIVGQSCMFCTVF 180

Query: 181 NSV-------------CWEKN---LTRMSVLELENGFSI-MEILGVYEWEGTNPMPPEFW 240
           N +             C+      L     L + +   I + ILGVYEWEGTNPMPPEFW
Sbjct: 181 NYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTNPMPPEFW 240

Query: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300
           LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT
Sbjct: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300

Query: 301 RHYCAK------------------------------------------------------ 313
           RHYCAK                                                      
Sbjct: 301 RHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHIDYEDHNSH 360

BLAST of Csor.00g208380 vs. NCBI nr
Match: XP_022946794.1 (LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata])

HSP 1 Score: 492 bits (1266), Expect = 1.15e-166
Identity = 263/407 (64.62%), Postives = 273/407 (67.08%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120
           DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120

Query: 121 MFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIF 180
           MFYFPPL              +   RK  + +        DG   L   V    +F T+F
Sbjct: 121 MFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNE-DGGWGL-HIVGQSCMFCTVF 180

Query: 181 NSV-------------CWEKN---LTRMSVLELENGFSI-MEILGVYEWEGTNPMPPEFW 240
           N +             C+      L     L + +   I + ILGVYEWEGTNPMPPEFW
Sbjct: 181 NYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTNPMPPEFW 240

Query: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300
           LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT
Sbjct: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300

Query: 301 RHYCAK------------------------------------------------------ 313
           RHYCAK                                                      
Sbjct: 301 RHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHIDYEDHNSH 360

BLAST of Csor.00g208380 vs. NCBI nr
Match: KAG6599054.1 (hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 466 bits (1199), Expect = 2.56e-161
Identity = 246/414 (59.42%), Postives = 272/414 (65.70%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEGA+DPYLFSSNNF+GRQTWDFEPD+GTPEERAEVEEARQNYYHNRFKVPC+S
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVED-VIDKETASIALRRATNFFAALQSPHGHWPAENAG 120
           DLFW+FQFLRERNFKQTIPIV+VE+ +I+KETASIALRRAT FFAALQS HGHWPAEN+G
Sbjct: 61  DLFWKFQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKFFAALQSHHGHWPAENSG 120

Query: 121 PMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTI 180
           PMFYFPPL              +   RK  + +         G     +   C  +  T+
Sbjct: 121 PMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQSC--MLCTV 180

Query: 181 FNSVCW--------EKNLTRMSVLELENGFSI---------MEILGVYEWEGTNPMPPEF 240
           FN +          +    R     L++G +I         + ILGVYEWEG NPMPPEF
Sbjct: 181 FNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGANPMPPEF 240

Query: 241 WLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSP 300
           WLLGKLLPFIPRTLFCYSRLTLLPMSYL+GKRFVGPLTPLILQLRQEIYTQPYNHIKW+P
Sbjct: 241 WLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYNHIKWNP 300

Query: 301 TRHYCAK----------------------------------------------------- 319
           TRHYCAK                                                     
Sbjct: 301 TRHYCAKEDKCFERSLFQKLAWDSLQYLGEPILNSMAFKKIRNKALQIAKCHIDYEDHNS 360

BLAST of Csor.00g208380 vs. NCBI nr
Match: KAG6599053.1 (Beta-amyrin synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458 bits (1179), Expect = 2.90e-158
Identity = 246/413 (59.56%), Postives = 274/413 (66.34%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEGA+DP LFSSNNFVGRQTW+FEPD+GT EERAEVEEARQNYYHNRFKV CN 
Sbjct: 1   MWRLKLGEGANDPCLFSSNNFVGRQTWNFEPDEGTAEERAEVEEARQNYYHNRFKVQCNG 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVED-VIDKETASIALRRATNFFAALQSPHGHWPAENAG 120
           DLFW+FQFLRERNFKQTIPIV+VE+ +I+KETASIALRRAT  FAALQSPHGHWPAENAG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIVKVEEAIINKETASIALRRATKLFAALQSPHGHWPAENAG 120

Query: 121 PMFYFPPLNSTARKSFVTHIVI----RMKMV---------DGDCTLWDKVVCFALFLTIF 180
           PMFYFPPL  +   +   HI+     R +++         DG   L   V    +  T+F
Sbjct: 121 PMFYFPPLVFSLYITRHLHIIFSEHHRKEILRYAYYHQNEDGGWGL-HIVGQSCMLCTVF 180

Query: 181 NSVCW--------EKNLTRMSVLELENGFSI---------MEILGVYEWEGTNPMPPEFW 240
           N +          +    R     L++G +I         + ILGVYEWEG NPMPPEFW
Sbjct: 181 NYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGANPMPPEFW 240

Query: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300
           LLGKLLPFIPR+L CYSRLTLLPMSYL+GKRFVGPLTPLILQLRQEIYTQPYN+IKWSPT
Sbjct: 241 LLGKLLPFIPRSLLCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYNNIKWSPT 300

Query: 301 RHYCAK------------------------------------------------------ 319
           RH+CAK                                                      
Sbjct: 301 RHFCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHIDYEDHNSH 360

BLAST of Csor.00g208380 vs. ExPASy TrEMBL
Match: A0A6J1G4X9 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450754 PE=3 SV=1)

HSP 1 Score: 492 bits (1266), Expect = 5.56e-167
Identity = 263/407 (64.62%), Postives = 273/407 (67.08%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120
           DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVEDVIDKETASIALRRATNFFAALQSPHGHWPAENAGP 120

Query: 121 MFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFLTIF 180
           MFYFPPL              +   RK  + +        DG   L   V    +F T+F
Sbjct: 121 MFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNE-DGGWGL-HIVGQSCMFCTVF 180

Query: 181 NSV-------------CWEKN---LTRMSVLELENGFSI-MEILGVYEWEGTNPMPPEFW 240
           N +             C+      L     L + +   I + ILGVYEWEGTNPMPPEFW
Sbjct: 181 NYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTNPMPPEFW 240

Query: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300
           LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT
Sbjct: 241 LLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKWSPT 300

Query: 301 RHYCAK------------------------------------------------------ 313
           RHYCAK                                                      
Sbjct: 301 RHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHIDYEDHNSH 360

BLAST of Csor.00g208380 vs. ExPASy TrEMBL
Match: A0A6J1G4T6 (lupeol synthase-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=4 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 7.18e-151
Identity = 240/416 (57.69%), Postives = 268/416 (64.42%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEG +DPYLFSSNNF+GRQTWDFEPDDGTP+ERAEVEEARQNYY NRFKV C+S
Sbjct: 1   MWRLKLGEGGNDPYLFSSNNFMGRQTWDFEPDDGTPQERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVID---------KETASIALRRATNFFAALQSPHG 120
           DLFW+FQFLRERNFKQTIPI+RVEDV+D         KET +IALRRATNFFAALQSPHG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIMRVEDVVDEDKKVAIIKKETVTIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPMFYFPPLNSTARKSFVTHIVI----RMKMV---------DGDCTLW-DKVV 180
           HWPAENAGPMFYFPPL  +   +   HIV     R +++         DG   L  +   
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CFALFLTIFNSVCW--------EKNLTRMSVLELENGFSI---------MEILGVYEWEG 240
           C  +  T+FN +          +    R     L++G +I         + ILGVYEWEG
Sbjct: 181 C--MLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEG 240

Query: 241 TNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQP 300
            NPMPPEFWLLGKLLPFIPR LFC+SRLTLLPM+YL+GKRFVG LTPLILQLRQEIYTQP
Sbjct: 241 ANPMPPEFWLLGKLLPFIPRNLFCHSRLTLLPMTYLFGKRFVGSLTPLILQLRQEIYTQP 300

Query: 301 YNHIKWSPTRHYCAK--------------------------------------------- 313
           YNHIKWSPTRHYCAK                                             
Sbjct: 301 YNHIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYIGEPILNSWAFKTIRNRALQIAKCH 360

BLAST of Csor.00g208380 vs. ExPASy TrEMBL
Match: A0A6J1G4L5 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 5.45e-149
Identity = 241/417 (57.79%), Postives = 269/417 (64.51%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEG +DPYLFSSNNF+GRQTWDFEPDDGTP+ERAEVEEARQNYY NRFKV C+S
Sbjct: 1   MWRLKLGEGGNDPYLFSSNNFMGRQTWDFEPDDGTPQERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVID---------KETASIALRRATNFFAALQSPHG 120
           DLFW+FQFLRERNFKQTIPI+RVEDV+D         KET +IALRRATNFFAALQSPHG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIMRVEDVVDEDKKVAIIKKETVTIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPMFYFPPLNSTARKSFVTHIVI----RMKMV---------DGDCTLW-DKVV 180
           HWPAENAGPMFYFPPL  +   +   HIV     R +++         DG   L  +   
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CFALFLTIFNSVCW--------EKNLTRMSVLELENGFSI---------MEILGVYEWEG 240
           C  +  T+FN +          +    R     L++G +I         + ILGVYEWEG
Sbjct: 181 C--MLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEG 240

Query: 241 TNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQP 300
            NPMPPEFWLLGKLLPFIPR LFC+SRLTLLPM+YL+GKRFVG LTPLILQLRQEIYTQP
Sbjct: 241 ANPMPPEFWLLGKLLPFIPRNLFCHSRLTLLPMTYLFGKRFVGSLTPLILQLRQEIYTQP 300

Query: 301 YNHIKWSPTRHYCAK--------------------------------------------- 314
           YNHIKWSPTRHYCAK                                             
Sbjct: 301 YNHIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYIGEPILNSWAFKTIRNRALQIAKCH 360

BLAST of Csor.00g208380 vs. ExPASy TrEMBL
Match: A0A6J1G4T2 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE=3 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 6.87e-148
Identity = 240/416 (57.69%), Postives = 268/416 (64.42%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEG +DPYLFSSNNF+GRQTWDFEPDDGTP+ERAEVEEARQNYY NRFKV C+S
Sbjct: 1   MWRLKLGEGGNDPYLFSSNNFMGRQTWDFEPDDGTPQERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVEDVID---------KETASIALRRATNFFAALQSPHG 120
           DLFW+FQFLRERNFKQTIPI+RVEDV+D         KET +IALRRATNFFAALQSPHG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIMRVEDVVDEDKKVAIIKKETVTIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPMFYFPPLNSTARKSFVTHIVI----RMKMV---------DGDCTLW-DKVV 180
           HWPAENAGPMFYFPPL  +   +   HIV     R +++         DG   L  +   
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLHIVFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CFALFLTIFNSVCW--------EKNLTRMSVLELENGFSI---------MEILGVYEWEG 240
           C  +  T+FN +          +    R     L++G +I         + ILGVYEWEG
Sbjct: 181 C--MLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEG 240

Query: 241 TNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQP 300
            NPMPPEFWLLGKLLPFIPR LFC+SRLTLLPM+YL+GKRFVG LTPLILQLRQEIYTQP
Sbjct: 241 ANPMPPEFWLLGKLLPFIPRNLFCHSRLTLLPMTYLFGKRFVGSLTPLILQLRQEIYTQP 300

Query: 301 YNHIKWSPTRHYCAK--------------------------------------------- 313
           YNHIKWSPTRHYCAK                                             
Sbjct: 301 YNHIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYIGEPILNSWAFKTIRNRALQIAKCH 360

BLAST of Csor.00g208380 vs. ExPASy TrEMBL
Match: A0A6J1KGL7 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705 PE=3 SV=1)

HSP 1 Score: 435 bits (1118), Expect = 2.30e-145
Identity = 238/422 (56.40%), Postives = 266/422 (63.03%), Query Frame = 0

Query: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60
           MWRL +GEGA+DPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYY NRFKV C+S
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  DLFWRFQFLRERNFKQTIPIVRVED---------VIDKETASIALRRATNFFAALQSPHG 120
           DLFW+FQFLR+RNFKQTIPIV+VE+         +I+KETA+ ALRRAT FFAALQS HG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENAGPMFYFPPL-------------------NSTARKSFV---------THIVIRM 180
           HWPAEN+GPMFYFPPL                   N   R S+           HI  + 
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 KMVDGDCTLWDKVVCFALFLTIFNSVCWEKNLTRMSVLELENGFSI---------MEILG 240
            M+   CT+++      L L +      +    R     L++G +I         + ILG
Sbjct: 181 CML---CTVFNY-----LQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILG 240

Query: 241 VYEWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQ 300
           VY+WEG NPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GKRFVGPLTPLILQLRQ
Sbjct: 241 VYQWEGANPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQ 300

Query: 301 EIYTQPYNHIKWSPTRHYCAK--------------------------------------- 313
           EIYTQ YN+IKWSPTRHYCAK                                       
Sbjct: 301 EIYTQSYNNIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRAL 360

BLAST of Csor.00g208380 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 312.0 bits (798), Expect = 5.5e-85
Identity = 175/416 (42.07%), Postives = 220/416 (52.88%), Query Frame = 0

Query: 1   MWRLTVGEG-ADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCN 60
           MWRL +GEG  DDPYLF++NNF GRQTW+F+PD G+PEER  V EAR+ +Y NRF V  +
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPDGGSPEERHSVVEARRIFYDNRFHVKAS 60

Query: 61  SDLFWRFQFLRERNFKQTIPIVRVED--VIDKETASIALRRATNFFAALQSPHGHWPAEN 120
           SDL WR QFLRE+ F+Q I  V+VED   +  ETA+ ALRR  +FF+ALQ+  GHWPAEN
Sbjct: 61  SDLLWRMQFLREKKFEQRIAPVKVEDSEKVTFETATSALRRGIHFFSALQASDGHWPAEN 120

Query: 121 AGPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVV--CFAL 180
           AGP+F+ PPL               S  RK  + +I    K   G    W   +     +
Sbjct: 121 AGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGG----WGLHIEGHSTM 180

Query: 181 FLTIFNSVCW-------------------EKNLTRMSVLELEN-GFSIMEILGVYEWEGT 240
           F T  N +C                    E  L+   V  + + G + + ILGV++W G+
Sbjct: 181 FCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLSILGVFDWSGS 240

Query: 241 NPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPY 300
           NPMPPEFW+L    P  P  ++ Y R+  LPMSYLYGKRFVGP+T LILQLR+E+Y QPY
Sbjct: 241 NPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKRFVGPITSLILQLRKELYLQPY 300

Query: 301 NHIKWSPTRHYCAKP--------------------------------------------- 314
             I W   RH CAK                                              
Sbjct: 301 EEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPFLARWPFNKLLREKALQLAMKH 360

BLAST of Csor.00g208380 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 304.7 bits (779), Expect = 8.9e-83
Identity = 170/421 (40.38%), Postives = 220/421 (52.26%), Query Frame = 0

Query: 1   MWRLTVGEG-ADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCN 60
           MW+L +  G  ++PYLFS+NNF+GRQTW+F+PD GT EE A VEEAR+ +Y +RF+V  +
Sbjct: 1   MWKLKIANGNKEEPYLFSTNNFLGRQTWEFDPDAGTVEELAAVEEARRKFYDDRFRVKAS 60

Query: 61  SDLFWRFQFLRERNFKQTIPIVRVEDV--IDKETASIALRRATNFFAALQSPHGHWPAEN 120
           SDL WR QFL+E+ F+Q IP  +VED   I  E A+ ALR+  NF +ALQ+  GHWPAEN
Sbjct: 61  SDLIWRMQFLKEKKFEQVIPPAKVEDANNITSEIATNALRKGVNFLSALQASDGHWPAEN 120

Query: 121 AGPMFYFPPL--------------NSTARKSFVTHIVIRMK-------MVDGDCTLWDKV 180
           AGP+F+ PPL                  R+  + +I             ++G+ T     
Sbjct: 121 AGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGWGLHIEGNST----- 180

Query: 181 VCFALFLTIFNSVCWE-----------KNLTRMSVLELENGFSI---------MEILGVY 240
               +F T  N +C                 R     L++G +          + ILGV+
Sbjct: 181 ----MFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLSILGVF 240

Query: 241 EWEGTNPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEI 300
           +W G+NPMPPEFW+L   LP  P  ++CY RL  +PMSYLYGKRFVGP++PLILQLR+EI
Sbjct: 241 DWSGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKRFVGPISPLILQLREEI 300

Query: 301 YTQPYNHIKWSPTRHYCAKP------------------LFT------------------- 314
           Y QPY  I W+  RH CAK                   +FT                   
Sbjct: 301 YLQPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPFLACWPFNKLLREKALG 360

BLAST of Csor.00g208380 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 293.5 bits (750), Expect = 2.0e-79
Identity = 165/416 (39.66%), Postives = 216/416 (51.92%), Query Frame = 0

Query: 1   MWRLTVGEG-ADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCN 60
           MW+L +GEG  +DPYLFSSNNFVGRQTW+F+P  GTPEERA VE+AR+NY  NR +V   
Sbjct: 1   MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGC 60

Query: 61  SDLFWRFQFLRERNFKQTIPIVRVED--VIDKETASIALRRATNFFAALQSPHGHWPAEN 120
           SDL WR QFL+E  F+Q IP V+++D   I  + A+ ALRRA +F++ALQS  GHWPAE 
Sbjct: 61  SDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYKNATDALRRAVSFYSALQSSDGHWPAEI 120

Query: 121 AGPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVV--CFAL 180
            G +F+ PPL              ++  RK  + HI        G    W   +     +
Sbjct: 121 TGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGG----WGLHIEGKSVM 180

Query: 181 FLTIFNSVCWEK-------------NLTRMSVLELEN-------GFSIMEILGVYEWEGT 240
           F T+ N +C                   R  +L+          G   + ILG+Y+W GT
Sbjct: 181 FCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDWSGT 240

Query: 241 NPMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPY 300
           NPMPPE WLL    P       CY+R+  +PMSYLYGKRFVGPLTPLI+ LR+E++ QPY
Sbjct: 241 NPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPLIMLLRKELHLQPY 300

Query: 301 NHIKWSPTRHYCAK-----------------------PLFT------------------- 314
             I W+  R  CAK                       P+ T                   
Sbjct: 301 EEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKKLVREKALRVAMEH 360

BLAST of Csor.00g208380 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-77
Identity = 165/411 (40.15%), Postives = 215/411 (52.31%), Query Frame = 0

Query: 1   MWRLTVGEG-ADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCN 60
           MW+L +G+G  +DP+LFSSNNFVGRQTW F+   G+PEERA VEEAR+ +  NRF+V   
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  SDLFWRFQFLRERNFKQTIPIVRVEDV--IDKETASIALRRATNFFAALQSPHGHWPAEN 120
           SDL WR QFLRE+ F+Q IP ++  ++  I  ET + ALRR   +F ALQ+  GHWP E 
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLKATNIEEITYETTTNALRRGVRYFTALQASDGHWPGEI 120

Query: 121 AGPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFL 180
            GP+F+ PPL              ++  RK  + HI       DG   L  +     +F 
Sbjct: 121 TGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQN-EDGGWGLHIESKS-VMFC 180

Query: 181 TIFNSVCW----------------EKNLTRMSVLELEN-GFSIMEILGVYEWEGTNPMPP 240
           T+ N +C                 +  L R  V+ + + G   + ILGVY+W GTNP PP
Sbjct: 181 TVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGTNPTPP 240

Query: 241 EFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKW 300
           E  +L   LP  P  + CYSR+  +PMSYLYGKRFVGP+TPLIL LR+E+Y +PY  I W
Sbjct: 241 ELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLILLLREELYLEPYEEINW 300

Query: 301 SPTRHYCAK-----------------------PLFT------------------------ 314
             +R   AK                       PL T                        
Sbjct: 301 KKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKLVREKALQLTMKHIHYED 360

BLAST of Csor.00g208380 vs. TAIR 10
Match: AT1G78970.1 (lupeol synthase 1 )

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-77
Identity = 165/411 (40.15%), Postives = 215/411 (52.31%), Query Frame = 0

Query: 1   MWRLTVGEG-ADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCN 60
           MW+L +G+G  +DP+LFSSNNFVGRQTW F+   G+PEERA VEEAR+ +  NRF+V   
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  SDLFWRFQFLRERNFKQTIPIVRVEDV--IDKETASIALRRATNFFAALQSPHGHWPAEN 120
           SDL WR QFLRE+ F+Q IP ++  ++  I  ET + ALRR   +F ALQ+  GHWP E 
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLKATNIEEITYETTTNALRRGVRYFTALQASDGHWPGEI 120

Query: 121 AGPMFYFPPL--------------NSTARKSFVTHIVIRMKMVDGDCTLWDKVVCFALFL 180
            GP+F+ PPL              ++  RK  + HI       DG   L  +     +F 
Sbjct: 121 TGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQN-EDGGWGLHIESKS-VMFC 180

Query: 181 TIFNSVCW----------------EKNLTRMSVLELEN-GFSIMEILGVYEWEGTNPMPP 240
           T+ N +C                 +  L R  V+ + + G   + ILGVY+W GTNP PP
Sbjct: 181 TVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGTNPTPP 240

Query: 241 EFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYNHIKW 300
           E  +L   LP  P  + CYSR+  +PMSYLYGKRFVGP+TPLIL LR+E+Y +PY  I W
Sbjct: 241 ELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLILLLREELYLEPYEEINW 300

Query: 301 SPTRHYCAK-----------------------PLFT------------------------ 314
             +R   AK                       PL T                        
Sbjct: 301 KKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKLVREKALQLTMKHIHYED 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E7DN636.2e-8942.93Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1[more]
Q8W3Z12.3e-8841.36Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1[more]
A8C9802.3e-8842.89Germanicol synthase OS=Rhizophora stylosa OX=98588 GN=M1 PE=1 SV=1[more]
A8CDT26.8e-8842.69Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1[more]
Q2XPU72.6e-8742.03Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6599056.11.92e-249100.00Beta-amyrin synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6599032.11.31e-17164.62hypothetical protein SDJN03_08810, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022946794.11.15e-16664.62LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata][more]
KAG6599054.12.56e-16159.42hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6599053.12.90e-15859.56Beta-amyrin synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1G4X95.56e-16764.62Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507... [more]
A0A6J1G4T67.18e-15157.69lupeol synthase-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111450753 PE... [more]
A0A6J1G4L55.45e-14957.79Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507... [more]
A0A6J1G4T26.87e-14857.69Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507... [more]
A0A6J1KGL72.30e-14556.40Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705... [more]
Match NameE-valueIdentityDescription
AT1G78950.15.5e-8542.07Terpenoid cyclases family protein [more]
AT1G78955.18.9e-8340.38camelliol C synthase 1 [more]
AT1G78960.12.0e-7939.66lupeol synthase 2 [more]
AT1G78970.22.5e-7740.15lupeol synthase 1 [more]
AT1G78970.12.5e-7740.15lupeol synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.50.10.20coord: 186..275
e-value: 3.3E-34
score: 120.3
coord: 276..315
e-value: 1.7E-11
score: 45.8
NoneNo IPR availablePANTHERPTHR11764:SF69TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 1..128
NoneNo IPR availablePANTHERPTHR11764:SF69TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 188..275
NoneNo IPR availablePANTHERPTHR11764:SF69TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 275..314
IPR032697Squalene cyclase, N-terminalPFAMPF13249SQHop_cyclase_Ncoord: 192..273
e-value: 5.3E-10
score: 38.9
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 188..275
IPR018333Squalene cyclasePANTHERPTHR11764TERPENE CYCLASE/MUTASE FAMILY MEMBERcoord: 1..128
coord: 275..314
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 192..312
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 10..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g208380.m01Csor.00g208380.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016104 triterpenoid biosynthetic process
cellular_component GO:0005811 lipid droplet
molecular_function GO:0042300 beta-amyrin synthase activity
molecular_function GO:0000250 lanosterol synthase activity
molecular_function GO:0016866 intramolecular transferase activity