Csor.00g205720 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g205720
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionsubtilisin-like protease SBT5.3
LocationCsor_Chr06: 3649096 .. 3652844 (-)
RNA-Seq ExpressionCsor.00g205720
SyntenyCsor.00g205720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTTCTTATATCATTTCTCCATTGCTTTTGTTCTTCTTTTTGCTTCATACATCATCTGTTCATACCAAAAAGGCAAGCATTCACCTCTCTCTCCTTTATTTTCAAATGGGTTTCCTTTTTGTTTTCATTTTAATTTATACTTCTTCTTTCTTGACTTAGTCTTATATTGTTTACTTGGGATCGGGATCGAGCACGTTTGGATCAAACCCGAGTATATACGATGTTCAACTTGCGACTGAATCTAAATATGATATATTAGGAACTGTGAAAGGAAGGTAAAAAATGTGTTCTTCAAAAGGTTCTATGATTTGATTAAGAAACTTATGATTAATGATTGTTTTTTTATGTTTCTTATGATGTATAGCAAAATGGCAGCGAAAGAATCTATTCTCTATACGTATAATAGATGTATTAATGGCTTTGCTGCTGTTCTTGATGACAATGAAGTCACGGCTCTTGCAAGTAAGAGTTGTTGGTTTCTGTACGATGATGTTTTAAATGTTTTGTTTTTAAAATGTTTTTTTTTTTGTTTTTTGTGTTTGCGAAATGTGACAGAGAATCCAAGTGTGGTGTCGGTTTTTGAAAATAGGGAAAGAAAATTGCATACAACACGATCATGGGGTTTTTTGGGGGTTGATAGTGGTCGAGGAATTCTTCAGAACTCCATTTGGAAGGCTTCTAAGTTTGGGGAAGATGTAATCATCGGGAACCTCGACACGGGTTTGCTCTCTTCTTCCCTTTTTTTTTTTATTTATTTTTATTTTTTTATGTGCTTGCGATGGTTCTGTACATGTAATAGCTCTAGCCGATATTGTCCAGTTTGTTATGTTATGTATCGCCTGCCGTCTGCCTCACGGCTTTAAAATGTCCAGAAGGGAACGATGGTAGAACTTGAGGGGATTTTGAGGGGAATGATTCTATATATGTAACAGCTTAAGCGCACTGCTAGCTGATCTTTAACTGTGAGCTTAGGCTCTTACTAGTATTTGAGCTAGTCATCAGATAGTGTCAGACACTGGTTAGAGTAGTGCTTGACCCTTCCTATAGTAGACACAGTTTTAAAACTGACAATATTAGTGGTGGGTATGGACTATTACAACTGGTATCACAACCAATCACCAATCATGTGAGGCACGAGTGGGAGTAGGGCTAGATCCTCCGCACAATATTAGCGGTAGGGTGGGCTTGAACAGTTACAAATGGTATTAGAGCGAGTCACTAATCATGCTAGACCCTCTGGGAGTCAAGCTACAAATGGTGTTAGAGCCTGTCAATCATGCTAGACCCTCTAGGAGTAGAGCTAGACCCTCACTATAGAACATATTTTAAAATCGTGAGGTTGACGTAACAGGCCATCGGACAGTGCAAGGCACGGGTGGATTATGAAGTCTTACATCCCTTAGATAAGGGAACGAGAGTGTGGAAACCTTTCCTTCACATTTTAAAATATTTATTAGCGGTGAGCTATAATTATAATATATGTGATGAGTTTGCATGGGGTTTGTATGAAGGCGTTTGGCCAGAGTCCGAGAGCTTCCACGATGCAGGCTATGGCCCTGTTCCTCCAAGGTGGATGGGAGCTTGTGAAGGCGGCTCCAAATTTCGATGCAACAGGTTCTTCCCCCACTTTAGCCTTAAAATCAAATTTACAAACACGCCATTTAACTAACCTTTACTTCTAGGAAGCTGATCGGAGCAAGATATTTCTACCGAGGATACGAAATGATCAATGGTCCTCTCAACATTAGCTCACTAAACGCACGAGACCATGAAGGCCATGGTACACACACATTGTCCACTGCCGGTGGCAACTTTGTCCAAGGAGCCAACGTGTTTGGCAATGGCAATGGGACAGCAAAAGGAGGCGCCCCCAAAGCTCGTGTTGCTGCCTACAAGGTATGCTGGCCTCAAGGCAAGTGTTCTGATGCCGATCTCTTAGCCGGTATCGAAGCCGCTATCAGCGACGGTGTCGACGTTCTCTCGATCTCTCTCGGTGCAACAGCTCAGGACTTTGCTAATGACCCAATTTCAGTAGGGGCATTCCATGCCATTCAACAAGGAATCATTGTAGTTTGCTCTGCCGGAAACGATGGCCCGCTCCCTGGGACTGTCACCAATGTATCTCCATGGATGTTCACTGTTGGAGCTAGTTCCATCGACCGAGGCTTCTTCAGTTACGTGTACCTCGGAAACAAGAAACAGATCAAGGGTTCGAGTCTTTCATCGGGAGGATTGCCTCGTGGTAAATTGTACCCTTTGATGAATTCTGTTAACGCAAAAGCTTCCAATGCCTCAGATGGACTCGCGTAAGTCGTAACATAACTAAATATCTAAATATTTGCCTTTAATAAAAGATCAAACACTAAATATTCACTTTGTTCCTACAGCCAACTTTGCGAGGAGGGATCACTTGATCCCATGAAGGCAAGAGGGAAGATTATAGTATGTCTTCGAGGAGACAACGGAAGAATGGACAAGAGTTTCGAGGTTCTTCGTGTCGGTGGTGTCGGTATGATTCTGGTTAACGACAAGATCAGCGGTTCGGGCATTGAAACTGATCCACACATGCTTCCTACTTCACATGTAAGCTATATTGATGGCCTCTCCATCGCTCAATATTTGAAATCCACCAAGTAAGGACTCTCATCTATGTGCTTGTCTATGAGAAAATGAGAAGGGAATGGGCTGAATATGTAGTTTTTGTGTTGTAAATTAAACCAGAAGGCCTGTGGCTACCATAACTCCGGTAAGGACAGAGATAGGGATTAAACCATCACCTGTCATGGCTACATTCTCGTCAAGAGGCCCTAATCACATAACAGAGGCCATCATCAAGGTTGGGTTTTTTAGTATCATCAAGTTTTGAGTATTTGAAAACTTCTCTCAAATGTTCGTGTATATGTTGGTGTAGCCTGATATAACAGCACCGGGTGTGAATATAATCGCATCTTTCACGAGAGCGGCAGCAGCAACAGACTTACCATTTGACAAACGTCGAGTGCCTTTCAACGTTCAATCCGGCACATCCATGTCATGCCCGCACATCTCAGGCGTTGCAGGCCTTCTTAAGAAACTTCATCCTACATGGAGTCCTGCGGCTATCAAATCTGCCATTATGACTACAGGTTTATTAAACTCGAGAAAAACAAACCTTAATTTATATTTTATTTGGTCTTGTAAGACTTTGATTTTGTTATGCAGCCAAAACGAGAGACAACACCAAGAACACAATGTTGGACTTCAACAAAGTGAAGGCTACCCCATTTGATTATGGTGCAGGACTTGTCCATCCAAACAATGCCATGGACCCGGGCCTCGTTTACGACACGACGATTGATGACTACTTGAACTTCTTATGTACACAGGGCTATAACTCACTCACGCTCAAGAAATTCTCTAATAAGCCATTTGTTTGCGCTAAGAACTTCGCAATCACAGACCTCAACTATCCATCCATCTCGGTCCCCAAGTTGCAAATTGGGGCACCGGTGACGGTAAATAGAAGAGTGAAGAATGTGGGAAGCGCAGGCACGTATGTGGCGCGGGTGAGGATGCCCAAGGGCATTACAGTTATGGTTGAGCCAAGTACGTTGCAATTTCACAGCGTTGGTGAAGAGAAGCCTTTCAAACTTGTATTTCATTACGCACAAAAAGTGCGGCGTCCTGGCTATGTTTTTGGGGCATTGGTATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATTGCAGTGAATTTGGTATGA

mRNA sequence

ATGGAGTTTTCTTATATCATTTCTCCATTGCTTTTGTTCTTCTTTTTGCTTCATACATCATCTGTTCATACCAAAAAGTCTTATATTGTTTACTTGGGATCGGGATCGAGCACGTTTGGATCAAACCCGAGTATATACGATGTTCAACTTGCGACTGAATCTAAATATGATATATTAGGAACTGTGAAAGGAAGCAAAATGGCAGCGAAAGAATCTATTCTCTATACGTATAATAGATGTATTAATGGCTTTGCTGCTGTTCTTGATGACAATGAAGTCACGGCTCTTGCAAAGAATCCAAGTGTGGTGTCGGTTTTTGAAAATAGGGAAAGAAAATTGCATACAACACGATCATGGGGTTTTTTGGGGGTTGATAGTGGTCGAGGAATTCTTCAGAACTCCATTTGGAAGGCTTCTAAGTTTGGGGAAGATGTAATCATCGGGAACCTCGACACGGGCGTTTGGCCAGAGTCCGAGAGCTTCCACGATGCAGGCTATGGCCCTGTTCCTCCAAGGTGGATGGGAGCTTGTGAAGGCGGCTCCAAATTTCGATGCAACAGGAAGCTGATCGGAGCAAGATATTTCTACCGAGGATACGAAATGATCAATGGTCCTCTCAACATTAGCTCACTAAACGCACGAGACCATGAAGGCCATGGTACACACACATTGTCCACTGCCGGTGGCAACTTTGTCCAAGGAGCCAACGTGTTTGGCAATGGCAATGGGACAGCAAAAGGAGGCGCCCCCAAAGCTCGTGTTGCTGCCTACAAGGTATGCTGGCCTCAAGGCAAGTGTTCTGATGCCGATCTCTTAGCCGGTATCGAAGCCGCTATCAGCGACGGTGTCGACGTTCTCTCGATCTCTCTCGGTGCAACAGCTCAGGACTTTGCTAATGACCCAATTTCAGTAGGGGCATTCCATGCCATTCAACAAGGAATCATTGTAGTTTGCTCTGCCGGAAACGATGGCCCGCTCCCTGGGACTGTCACCAATGTATCTCCATGGATGTTCACTGTTGGAGCTAGTTCCATCGACCGAGGCTTCTTCAGTTACGTGTACCTCGGAAACAAGAAACAGATCAAGGGTTCGAGTCTTTCATCGGGAGGATTGCCTCGTGGTAAATTGTACCCTTTGATGAATTCTGTTAACGCAAAAGCTTCCAATGCCTCAGATGGACTCGCGCAACTTTGCGAGGAGGGATCACTTGATCCCATGAAGGCAAGAGGGAAGATTATAGTATGTCTTCGAGGAGACAACGGAAGAATGGACAAGAGTTTCGAGGTTCTTCGTGTCGGTGGTGTCGGTATGATTCTGGTTAACGACAAGATCAGCGGTTCGGGCATTGAAACTGATCCACACATGCTTCCTACTTCACATGTAAGCTATATTGATGGCCTCTCCATCGCTCAATATTTGAAATCCACCAAAAGGCCTGTGGCTACCATAACTCCGGTAAGGACAGAGATAGGGATTAAACCATCACCTGTCATGGCTACATTCTCGTCAAGAGGCCCTAATCACATAACAGAGGCCATCATCAAGCCTGATATAACAGCACCGGGTGTGAATATAATCGCATCTTTCACGAGAGCGGCAGCAGCAACAGACTTACCATTTGACAAACGTCGAGTGCCTTTCAACGTTCAATCCGGCACATCCATGTCATGCCCGCACATCTCAGGCGTTGCAGGCCTTCTTAAGAAACTTCATCCTACATGGAGTCCTGCGGCTATCAAATCTGCCATTATGACTACAGCCAAAACGAGAGACAACACCAAGAACACAATGTTGGACTTCAACAAAGTGAAGGCTACCCCATTTGATTATGGTGCAGGACTTGTCCATCCAAACAATGCCATGGACCCGGGCCTCGTTTACGACACGACGATTGATGACTACTTGAACTTCTTATGTACACAGGGCTATAACTCACTCACGCTCAAGAAATTCTCTAATAAGCCATTTGTTTGCGCTAAGAACTTCGCAATCACAGACCTCAACTATCCATCCATCTCGGTCCCCAAGTTGCAAATTGGGGCACCGGTGACGGTAAATAGAAGAGTGAAGAATGTGGGAAGCGCAGGCACGTATGTGGCGCGGGTGAGGATGCCCAAGGGCATTACAGTTATGGTTGAGCCAAGTACGTTGCAATTTCACAGCGTTGGTGAAGAGAAGCCTTTCAAACTTGTATTTCATTACGCACAAAAAGTGCGGCGTCCTGGCTATGTTTTTGGGGCATTGGTATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATTGCAGTGAATTTGGTATGA

Coding sequence (CDS)

ATGGAGTTTTCTTATATCATTTCTCCATTGCTTTTGTTCTTCTTTTTGCTTCATACATCATCTGTTCATACCAAAAAGTCTTATATTGTTTACTTGGGATCGGGATCGAGCACGTTTGGATCAAACCCGAGTATATACGATGTTCAACTTGCGACTGAATCTAAATATGATATATTAGGAACTGTGAAAGGAAGCAAAATGGCAGCGAAAGAATCTATTCTCTATACGTATAATAGATGTATTAATGGCTTTGCTGCTGTTCTTGATGACAATGAAGTCACGGCTCTTGCAAAGAATCCAAGTGTGGTGTCGGTTTTTGAAAATAGGGAAAGAAAATTGCATACAACACGATCATGGGGTTTTTTGGGGGTTGATAGTGGTCGAGGAATTCTTCAGAACTCCATTTGGAAGGCTTCTAAGTTTGGGGAAGATGTAATCATCGGGAACCTCGACACGGGCGTTTGGCCAGAGTCCGAGAGCTTCCACGATGCAGGCTATGGCCCTGTTCCTCCAAGGTGGATGGGAGCTTGTGAAGGCGGCTCCAAATTTCGATGCAACAGGAAGCTGATCGGAGCAAGATATTTCTACCGAGGATACGAAATGATCAATGGTCCTCTCAACATTAGCTCACTAAACGCACGAGACCATGAAGGCCATGGTACACACACATTGTCCACTGCCGGTGGCAACTTTGTCCAAGGAGCCAACGTGTTTGGCAATGGCAATGGGACAGCAAAAGGAGGCGCCCCCAAAGCTCGTGTTGCTGCCTACAAGGTATGCTGGCCTCAAGGCAAGTGTTCTGATGCCGATCTCTTAGCCGGTATCGAAGCCGCTATCAGCGACGGTGTCGACGTTCTCTCGATCTCTCTCGGTGCAACAGCTCAGGACTTTGCTAATGACCCAATTTCAGTAGGGGCATTCCATGCCATTCAACAAGGAATCATTGTAGTTTGCTCTGCCGGAAACGATGGCCCGCTCCCTGGGACTGTCACCAATGTATCTCCATGGATGTTCACTGTTGGAGCTAGTTCCATCGACCGAGGCTTCTTCAGTTACGTGTACCTCGGAAACAAGAAACAGATCAAGGGTTCGAGTCTTTCATCGGGAGGATTGCCTCGTGGTAAATTGTACCCTTTGATGAATTCTGTTAACGCAAAAGCTTCCAATGCCTCAGATGGACTCGCGCAACTTTGCGAGGAGGGATCACTTGATCCCATGAAGGCAAGAGGGAAGATTATAGTATGTCTTCGAGGAGACAACGGAAGAATGGACAAGAGTTTCGAGGTTCTTCGTGTCGGTGGTGTCGGTATGATTCTGGTTAACGACAAGATCAGCGGTTCGGGCATTGAAACTGATCCACACATGCTTCCTACTTCACATGTAAGCTATATTGATGGCCTCTCCATCGCTCAATATTTGAAATCCACCAAAAGGCCTGTGGCTACCATAACTCCGGTAAGGACAGAGATAGGGATTAAACCATCACCTGTCATGGCTACATTCTCGTCAAGAGGCCCTAATCACATAACAGAGGCCATCATCAAGCCTGATATAACAGCACCGGGTGTGAATATAATCGCATCTTTCACGAGAGCGGCAGCAGCAACAGACTTACCATTTGACAAACGTCGAGTGCCTTTCAACGTTCAATCCGGCACATCCATGTCATGCCCGCACATCTCAGGCGTTGCAGGCCTTCTTAAGAAACTTCATCCTACATGGAGTCCTGCGGCTATCAAATCTGCCATTATGACTACAGCCAAAACGAGAGACAACACCAAGAACACAATGTTGGACTTCAACAAAGTGAAGGCTACCCCATTTGATTATGGTGCAGGACTTGTCCATCCAAACAATGCCATGGACCCGGGCCTCGTTTACGACACGACGATTGATGACTACTTGAACTTCTTATGTACACAGGGCTATAACTCACTCACGCTCAAGAAATTCTCTAATAAGCCATTTGTTTGCGCTAAGAACTTCGCAATCACAGACCTCAACTATCCATCCATCTCGGTCCCCAAGTTGCAAATTGGGGCACCGGTGACGGTAAATAGAAGAGTGAAGAATGTGGGAAGCGCAGGCACGTATGTGGCGCGGGTGAGGATGCCCAAGGGCATTACAGTTATGGTTGAGCCAAGTACGTTGCAATTTCACAGCGTTGGTGAAGAGAAGCCTTTCAAACTTGTATTTCATTACGCACAAAAAGTGCGGCGTCCTGGCTATGTTTTTGGGGCATTGGTATGGTCAGATGGGAAGCATTTTGTTAGAAGTCCTATTGCAGTGAATTTGGTATGA

Protein sequence

MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Homology
BLAST of Csor.00g205720 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 1.2e-231
Identity = 408/766 (53.26%), Postives = 531/766 (69.32%), Query Frame = 0

Query: 11  LLFFFLLHTSSVH-----TKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGS 70
           LL   L+H SS H        SY+VY G+ S          D     E+ YD LG+  GS
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMD--RVKETHYDFLGSFTGS 69

Query: 71  KMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVD 130
           +  A ++I Y+Y + INGFAA LD +    ++K+P VVSVF N+  KLHTTRSW FLG++
Sbjct: 70  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 129

Query: 131 SGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEG--GSKF 190
               +  +SIW+ ++FGED II NLDTGVWPES+SF D G GP+P RW G C+    + F
Sbjct: 130 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATF 189

Query: 191 RCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNG 250
            CNRKLIGARYF +GY    G LN S  + RD +GHG+HTLSTA G+FV G ++FG GNG
Sbjct: 190 HCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249

Query: 251 TAKGGAPKARVAAYKVCWPQGK---CSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 310
           TAKGG+P+ARVAAYKVCWP  K   C DAD+LA  +AAI DG DV+S+SLG     F ND
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309

Query: 311 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 370
            +++G+FHA ++ I+VVCSAGN GP   TV+NV+PW  TVGAS++DR F S + LGN K 
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369

Query: 371 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 430
            KG SLSS  LP  K YP+M SVNAKA NAS   AQLC+ GSLDP+K +GKI+VCLRG N
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429

Query: 431 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 490
           GR++K   V   GG+GM+L N  ++G+ +  DPH+LP + ++  D  ++++Y+  TK+P+
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489

Query: 491 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 550
           A ITP RT++G+KP+PVMA+FSS+GP+ +   I+KPDITAPGV++IA++T A + T+  F
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549

Query: 551 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 610
           D RR+ FN  SGTSMSCPHISG+AGLLK  +P+WSPAAI+SAIMTTA   D+    + + 
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609

Query: 611 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 670
             +KATPF +GAG V PN A++PGLVYD  I DYLNFLC+ GYN+  +  FS   F C+ 
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669

Query: 671 -NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 730
              ++ +LNYPSI+VP L   + VTV+R VKNVG    Y  +V  P+G+ V V+P++L F
Sbjct: 670 PKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 729

Query: 731 HSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 766
             VGE+K FK++   ++     GYVFG LVWSD KH VRSPI V L
Sbjct: 730 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Csor.00g205720 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 3.0e-230
Identity = 418/766 (54.57%), Postives = 514/766 (67.10%), Query Frame = 0

Query: 7   ISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQL--ATESKYDILGTVKG 66
           +S LLL   L  + +   KKSYIVYLGS +      P I    L     S    L +  G
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHL----PQISSAHLDGVAHSHRTFLASFVG 80

Query: 67  SKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGV 126
           S   AKE+I Y+Y R INGFAA+LD+NE   +AK+P VVSVF N+ RKLHTT SW F+ +
Sbjct: 81  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 140

Query: 127 DSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFR 186
                + ++S+W  + +GED II NLDTGVWPES+SF D GYG VP RW G C       
Sbjct: 141 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVP 200

Query: 187 CNRKLIGARYFYRGYEMING-PLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNG 246
           CNRKLIGARYF +GY    G P N S    RDH+GHG+HTLSTA GNFV GANVFG GNG
Sbjct: 201 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 260

Query: 247 TAKGGAPKARVAAYKVCWPQ---GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 306
           TA GG+PKARVAAYKVCWP     +C DAD+LA IEAAI DGVDVLS S+G  A D+ +D
Sbjct: 261 TASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 320

Query: 307 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 366
            I++G+FHA++ G+ VVCSAGN GP  GTV+NV+PW+ TVGASS+DR F ++V L N + 
Sbjct: 321 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 380

Query: 367 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 426
            KG+SLS   LP  K+Y L+++ +A  +N +   A LC++GSLDP K +GKI+VCLRGDN
Sbjct: 381 FKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 440

Query: 427 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 486
            R+DK  +    G  GM+L NDK SG+ I +D H+LP S + Y DG ++  YL STK P 
Sbjct: 441 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 500

Query: 487 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 546
             I      +  KP+P MA+FSSRGPN IT  I+KPDITAPGVNIIA+FT A   TDL  
Sbjct: 501 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 560

Query: 547 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 606
           D RR PFN +SGTSMSCPHISGV GLLK LHP WSPAAI+SAIMTT++TR+N +  M+D 
Sbjct: 561 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 620

Query: 607 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKP-FVCA 666
           +  KA PF YG+G V PN A  PGLVYD T  DYL+FLC  GYN+  ++ F+  P + C 
Sbjct: 621 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 680

Query: 667 KNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 726
           +   + D NYPSI+VP L     +TV R++KNVG   TY AR R P G+ V VEP  L F
Sbjct: 681 QGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTF 740

Query: 727 HSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 766
           +  GE K F++       V   GYVFG L W+D  H+VRSPI V L
Sbjct: 741 NKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Csor.00g205720 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 721.8 bits (1862), Expect = 7.9e-207
Identity = 398/785 (50.70%), Postives = 520/785 (66.24%), Query Frame = 0

Query: 6   IISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGS 65
           I+S   LF FLL   +  +KK YIVY+G+ S   G +P+  D++LAT+S YD+LG++ GS
Sbjct: 11  ILSSFFLFTFLLAAVN-GSKKCYIVYMGAHS--HGPSPTSADLELATDSHYDLLGSIFGS 70

Query: 66  KMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVD 125
           +  AKE+I+Y+YNR INGFAA+L++ E   +AKNP+VVSVF ++E KLHTTRSW FLG+ 
Sbjct: 71  REKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLH 130

Query: 126 SGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGA-CE-----G 185
             RG  QNS W+  +FGE+ IIGN+DTGVWPES+SF D GYG VP +W G  C+     G
Sbjct: 131 R-RG--QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPG 190

Query: 186 GSKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFG 245
             K  CNRKLIGARY+ + +E  NG L+     ARD  GHGTHTLSTAGGNFV GA VF 
Sbjct: 191 SMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFA 250

Query: 246 NGNGTAKGGAPKARVAAYKVCW---PQGKCSDADLLAGIEAAISDGVDVLSISLG----A 305
            GNGTAKGG+P+ARVAAYKVCW       C  AD+LA I+ AI DGVDV+++S G     
Sbjct: 251 VGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVV 310

Query: 306 TAQDFANDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSY 365
           TA+    D IS+GAFHAI + I++V SAGNDGP PGTV NV+PW+FT+ AS++DR F S 
Sbjct: 311 TAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSN 370

Query: 366 VYLGNKKQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKI 425
           + + N + I+G+SL    LP  + + L+ S +AK +NA+   AQLC  G+LD  K  GKI
Sbjct: 371 LTI-NNQLIEGASLFV-NLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKI 430

Query: 426 IVCLR-GDNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQ 485
           ++C R G    + +  E L  G  GMIL N   +G  +  +PH+  T +       S   
Sbjct: 431 VLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPH 490

Query: 486 YLKST-----KRPVAT-----ITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAP 545
            +K+T       P+ T     ++  RT  G KP+PVMA+FSSRGPN I  +I+KPD+TAP
Sbjct: 491 GVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAP 550

Query: 546 GVNIIASFTRAAAATDLPFDKRR-VPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIK 605
           GVNI+A+++  A+A+ L  D RR   FNV  GTSMSCPH SG+AGLLK  HP+WSPAAIK
Sbjct: 551 GVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIK 610

Query: 606 SAIMTTAKTRDNTKNTMLD-FNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLC 665
           SAIMTTA T DNT   + D F+K  A  F YG+G V P+ A++PGLVYD ++ DYLNFLC
Sbjct: 611 SAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLC 670

Query: 666 TQGYNSLTLKKFS-NKPFVCAKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTY 725
             GY+   +   + N+ F+C+ + ++ DLNYPSI++P L++  PVT+ R V NVG   TY
Sbjct: 671 ASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTY 730

Query: 726 VARVRMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVR 764
               R P G ++ V P +L F  +GE K FK++   +    R  Y FG L W+DGKH VR
Sbjct: 731 TVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVR 786

BLAST of Csor.00g205720 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.8e-174
Identity = 343/767 (44.72%), Postives = 468/767 (61.02%), Query Frame = 0

Query: 10  LLLFFFLLHTSSVHTKK-SYIVYLGSGS--STFGSNPSIYDVQLATESKYDILGTVKGSK 69
           LLL     H SS  + + +YIV++      S+F  + + YD  L +              
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS-------------- 72

Query: 70  MAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDS 129
           ++    +LYTY   I+GF+  L   E  +L   P V+SV      +LHTTR+  FLG+D 
Sbjct: 73  ISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 132

Query: 130 GRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFR-- 189
                   ++  +    DV++G LDTGVWPES+S+ D G+GP+P  W G CE G+ F   
Sbjct: 133 HTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 192

Query: 190 -CNRKLIGARYFYRGYEMINGPLNIS--SLNARDHEGHGTHTLSTAGGNFVQGANVFGNG 249
            CNRKLIGAR+F RGYE   GP++ S  S + RD +GHGTHT STA G+ V+GA++ G  
Sbjct: 193 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 252

Query: 250 NGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDP 309
           +GTA+G AP+ARVA YKVCW  G C  +D+LA I+ AI+D V+VLS+SLG    D+  D 
Sbjct: 253 SGTARGMAPRARVAVYKVCW-LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDG 312

Query: 310 ISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQI 369
           +++GAF A+++GI+V CSAGN GP   +++NV+PW+ TVGA ++DR F +   LGN K  
Sbjct: 313 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 372

Query: 370 KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNG 429
            G SL  G     KL P + + N  ASNA++G   LC  G+L P K +GKI++C RG N 
Sbjct: 373 TGVSLFKGEALPDKLLPFIYAGN--ASNATNG--NLCMTGTLIPEKVKGKIVMCDRGINA 432

Query: 430 RMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVA 489
           R+ K   V   GGVGMIL N   +G  +  D H+LP + V    G  I  Y+ +   P A
Sbjct: 433 RVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTA 492

Query: 490 TITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFD 549
           +I+ + T +G+KPSPV+A FSSRGPN IT  I+KPD+ APGVNI+A++T AA  T L  D
Sbjct: 493 SISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD 552

Query: 550 KRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFN 609
            RRV FN+ SGTSMSCPH+SG+A LLK +HP WSPAAI+SA+MTTA         +LD  
Sbjct: 553 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 612

Query: 610 KVK-ATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC-- 669
             K +TPFD+GAG V P  A +PGL+YD T +DYL FLC   Y S  ++  S + + C  
Sbjct: 613 TGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 672

Query: 670 AKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRM-PKGITVMVEPSTL 729
           +K++++ DLNYPS +V    +GA     R V +VG AGTY  +V     G+ + VEP+ L
Sbjct: 673 SKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 732

Query: 730 QFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVN 765
            F    E+K + + F            FG++ WSDGKH V SP+A++
Sbjct: 733 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Csor.00g205720 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.2e-167
Identity = 336/785 (42.80%), Postives = 476/785 (60.64%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGS--STFGSNPSIYDVQLATESKYDI 60
           M  S I+  L L F  +  ++    K++I  +  GS  S F ++   Y  + A ES+   
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESR--- 60

Query: 61  LGTVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRS 120
                         I++ Y+   +GF+AV+  +E   L  +P+V++VFE+R R+LHTTRS
Sbjct: 61  --------------IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 WGFLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACE 180
             FLG+ + +G     +W  S +G DVIIG  DTG+WPE  SF D   GP+P RW G CE
Sbjct: 121 PQFLGLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCE 180

Query: 181 GGSKF---RCNRKLIGARYFYRGYE-MINGPLN--ISSLNARDHEGHGTHTLSTAGGNFV 240
            G++F    CNRK+IGAR+F +G +  + G +N  +  L+ RD +GHGTHT STA G   
Sbjct: 181 SGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHA 240

Query: 241 QGANVFGNGNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLG- 300
             A++ G  +G AKG APKAR+AAYKVCW    C D+D+LA  +AA+ DGVDV+SIS+G 
Sbjct: 241 FKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGG 300

Query: 301 --ATAQDFANDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGF 360
                  +  DPI++G++ A  +GI V  SAGN+GP   +VTN++PW+ TVGAS+IDR F
Sbjct: 301 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 360

Query: 361 FSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKAR 420
            +   LG+  +++G SL +G    G+++P++    +  S+AS     LC E +LDP + R
Sbjct: 361 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVR 420

Query: 421 GKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSI 480
           GKI++C RG + R+ K   V + GGVGMIL N   +G G+  D H++P   V   +G  I
Sbjct: 421 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 480

Query: 481 AQYLKSTKRPVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASF 540
             Y  S   P+A+I    T +GIKP+PV+A+FS RGPN ++  I+KPD+ APGVNI+A++
Sbjct: 481 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 540

Query: 541 TRAAAATDLPFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKT 600
           T A   T LP D R+  FN+ SGTSM+CPH+SG A LLK  HP WSPA I+SA+MTT   
Sbjct: 541 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 600

Query: 601 RDNTKNTMLDFNKVK-ATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTL 660
            DN+  +++D +  K ATP+DYG+G ++   AM+PGLVYD T DDY+ FLC+ GY   T+
Sbjct: 601 VDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI 660

Query: 661 KKFSNKPFVC--AKNFAITDLNYPSISV--PKLQIG-APVTVNRRVKNVGSA-GTYVARV 720
           +  +  P  C   +  +  +LNYPSI+   P  + G    TV R   NVG A   Y AR+
Sbjct: 661 QVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 720

Query: 721 RMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQK---VRRPGYVFGALVWSD-GKHFVR 764
             P+G+TV V+P  L F S  + + + +      +   +   G VFG++ W D GKH VR
Sbjct: 721 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 758

BLAST of Csor.00g205720 vs. NCBI nr
Match: KAG6596783.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1538 bits (3983), Expect = 0.0
Identity = 766/766 (100.00%), Postives = 766/766 (100.00%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120
           TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180
           FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG
Sbjct: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180

Query: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240
           SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540
           ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660

Query: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of Csor.00g205720 vs. NCBI nr
Match: XP_022949775.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 1526 bits (3952), Expect = 0.0
Identity = 760/766 (99.22%), Postives = 762/766 (99.48%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLLH SSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHASSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120
           TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180
           FLGVDSGRGI QNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVP RWMGACEGG
Sbjct: 121 FLGVDSGRGIPQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGG 180

Query: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240
           SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540
           ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC+K
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSK 660

Query: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
            VGEEKPFKLVFHYAQ+VRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 GVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of Csor.00g205720 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 1450 bits (3754), Expect = 0.0
Identity = 725/766 (94.65%), Postives = 738/766 (96.34%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLL TSSVHTKKSYIVYLGS S     NPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSES-----NPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120
           TVKGSK+AAKESILYTYNRCINGFAAVLDD EVTALAKNPSVVS+FENRERKLHTTRSW 
Sbjct: 61  TVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWS 120

Query: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180
           FLGV S RGI  NSIWKASKFGEDVIIGNLDTGVWPES SFHD+GYGPVP RWMGACEGG
Sbjct: 121 FLGVGSDRGIPWNSIWKASKFGEDVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGG 180

Query: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240
           S F CNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFV+GANVFGN
Sbjct: 181 SNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA AQDFA+D
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           +KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGS IETD HMLPTSHVSYIDGLSIA+YLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 480

Query: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540
           A+ITPVRTEIGIKPSPVMA FSSRGPNHI EA+IKPDI+APGVNIIASFT+A AATDLPF
Sbjct: 481 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHI+GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC QGYNSLTLKKFSNKPFVCAK
Sbjct: 601 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 660

Query: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFA TDLNYPSISVPKLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           SVGEEK FKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 761

BLAST of Csor.00g205720 vs. NCBI nr
Match: KAG6596782.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1242 bits (3213), Expect = 0.0
Identity = 612/753 (81.27%), Postives = 673/753 (89.38%), Query Frame = 0

Query: 19  TSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYN 78
           T++V TKKSYIVYLGS S  FG NPSIYDVQLATES+YDILG+VKGSK+AAK+SILY+YN
Sbjct: 17  TAAVPTKKSYIVYLGSHS--FGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYN 76

Query: 79  RCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGILQNSIWKA 138
           R INGFAAVLD+ E TALAKNPSVVSVFEN+ERKLHTTRSWGFLGVDS RGI +NSIWKA
Sbjct: 77  RYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPRNSIWKA 136

Query: 139 SKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFRCNRKLIGARYFYRG 198
           ++FGED IIGNLDTGVWPES SF+DAGYGPVP RW GACEGGSKFRCN KLIGARYFYRG
Sbjct: 137 ARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNSKLIGARYFYRG 196

Query: 199 YEMINGPL---NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVA 258
           ++   GPL   NIS ++ARDHEGHG+HTLSTAGGNFV G NVFGNGNGTAKGG+PKARV 
Sbjct: 197 FQAAEGPLRTHNISFVSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPKARVV 256

Query: 259 AYKVCWPQ--GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQG 318
           AYKVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FAND IS+GAFHA+Q G
Sbjct: 257 AYKVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHG 316

Query: 319 IIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPR 378
           I+VV SAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKKQ++GSSLSS  LP 
Sbjct: 317 IVVVSSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQLRGSSLSSSRLPA 376

Query: 379 GKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVG 438
           GK YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N R+ K FEVLRVG
Sbjct: 377 GKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVLRVG 436

Query: 439 GVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIK 498
           GVGM+LVN++  GS +  DPH+LP SH+SY DG+SIAQYL STK PVA+IT   TEIGIK
Sbjct: 437 GVGMVLVNNQTDGSALLADPHILPASHISYADGVSIAQYLSSTKTPVASITHASTEIGIK 496

Query: 499 PSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGT 558
           PSP+MA+FSSRGP+ IT+A+IKPDITAPGVNIIAS T+   A++LPFDKRRV FNV+SGT
Sbjct: 497 PSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTKDVTASELPFDKRRVSFNVESGT 556

Query: 559 SMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAG 618
           SMSCPHISGVAGLLK LHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF KVKATPFDYGAG
Sbjct: 557 SMSCPHISGVAGLLKTLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFTKVKATPFDYGAG 616

Query: 619 LVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAKNFAITDLNYPSIS 678
            VHPN+AMDPGLVYDTT+DDYLNFLCTQGYNS TLKKFSNKPFVCAK FAITDLNYPSIS
Sbjct: 617 HVHPNDAMDPGLVYDTTVDDYLNFLCTQGYNSRTLKKFSNKPFVCAKKFAITDLNYPSIS 676

Query: 679 VPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKPFKLVFH 738
           VPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS LQFH+VGEEKPFKLVFH
Sbjct: 677 VPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSKLQFHNVGEEKPFKLVFH 736

Query: 739 YAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           YA K+RR GYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 737 YAHKLRRQGYVFGALVWSDGKHFVRSPIAVNLV 767

BLAST of Csor.00g205720 vs. NCBI nr
Match: XP_023540421.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1235 bits (3196), Expect = 0.0
Identity = 616/768 (80.21%), Postives = 675/768 (87.89%), Query Frame = 0

Query: 4   SYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVK 63
           SY+ISPLLLFFF+L T +V TKKSYIVYLGS S  FG NPSIYDVQLATES+Y ILG+VK
Sbjct: 8   SYVISPLLLFFFMLQTVAVPTKKSYIVYLGSHS--FGPNPSIYDVQLATESQYHILGSVK 67

Query: 64  GSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLG 123
           GSK+AAK+SILY+YNR INGFAAVLD+ E TALAKNPSVVSVFEN+ERKLHTTRSWGFLG
Sbjct: 68  GSKVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLG 127

Query: 124 VDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKF 183
           VDS RGI +NSIWKA++FGED IIGNLDTGVWPES SF+DAGYGPVP RW GACEGGSKF
Sbjct: 128 VDSDRGIPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKF 187

Query: 184 RCNRKLIGARYFYRGYEMINGPL---NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 243
           RCNRKLIGARYFYRG++    P    NIS  +ARDHEGHG+HTLSTAGGNFV G NVFGN
Sbjct: 188 RCNRKLIGARYFYRGFQASERPWTKQNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGN 247

Query: 244 GNGTAKGGAPKARVAAYKVCWPQ--GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFA 303
           GNGTAKGG+PKARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FA
Sbjct: 248 GNGTAKGGSPKARVVAYKVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFA 307

Query: 304 NDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNK 363
           ND IS+GAFHA+Q GI+VV SAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNK
Sbjct: 308 NDAISIGAFHAVQHGIVVVSSAGNDGPSPGSVSNVSPWMITVGASTIDRDFVSYVALGNK 367

Query: 364 KQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRG 423
           KQ++GSSLSS  LP GK YPLM +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG
Sbjct: 368 KQLRGSSLSSSRLPAGKFYPLMKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRG 427

Query: 424 DNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKR 483
           +N R+ K FEVLRVGGVGM+LVN++  GS +  DPH+LP SH+SY DG+SIAQYL STK 
Sbjct: 428 ENARLSKGFEVLRVGGVGMVLVNNQTDGSALLADPHILPASHLSYADGVSIAQYLSSTKT 487

Query: 484 PVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDL 543
           PVA+IT   TE+GIKPS VMA+FSSRGP  IT+AIIKPDITAPGVNIIAS T+   A++L
Sbjct: 488 PVASITHASTEMGIKPSAVMASFSSRGPELITQAIIKPDITAPGVNIIASVTKDVTASEL 547

Query: 544 PFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTML 603
           PFDKRRV FNV+SGTSMSCPHISGVAGLLK LHPTWSPAAIKSAIMTTAKTRDNTKNTML
Sbjct: 548 PFDKRRVSFNVESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAIMTTAKTRDNTKNTML 607

Query: 604 DFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC 663
           DF KVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLCT+GY+SLTLKKFS KPFVC
Sbjct: 608 DFTKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCTRGYSSLTLKKFSKKPFVC 667

Query: 664 AKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQ 723
           AKNF  TD NYPSI VP+LQIG  VTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS LQ
Sbjct: 668 AKNFTTTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSKLQ 727

Query: 724 FHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           FHSVGEEKPFKLVFHYA K+RR GYVFGALVWSDGKHFVRS IAVNLV
Sbjct: 728 FHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNLV 773

BLAST of Csor.00g205720 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 1526 bits (3952), Expect = 0.0
Identity = 760/766 (99.22%), Postives = 762/766 (99.48%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLLH SSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHASSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120
           TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180
           FLGVDSGRGI QNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVP RWMGACEGG
Sbjct: 121 FLGVDSGRGIPQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGG 180

Query: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240
           SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540
           ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC+K
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSK 660

Query: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
            VGEEKPFKLVFHYAQ+VRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 GVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of Csor.00g205720 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1450 bits (3754), Expect = 0.0
Identity = 725/766 (94.65%), Postives = 738/766 (96.34%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLL TSSVHTKKSYIVYLGS S     NPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSES-----NPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120
           TVKGSK+AAKESILYTYNRCINGFAAVLDD EVTALAKNPSVVS+FENRERKLHTTRSW 
Sbjct: 61  TVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWS 120

Query: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180
           FLGV S RGI  NSIWKASKFGEDVIIGNLDTGVWPES SFHD+GYGPVP RWMGACEGG
Sbjct: 121 FLGVGSDRGIPWNSIWKASKFGEDVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGG 180

Query: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240
           S F CNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFV+GANVFGN
Sbjct: 181 SNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA AQDFA+D
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           +KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGS IETD HMLPTSHVSYIDGLSIA+YLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 480

Query: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540
           A+ITPVRTEIGIKPSPVMA FSSRGPNHI EA+IKPDI+APGVNIIASFT+A AATDLPF
Sbjct: 481 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHI+GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC QGYNSLTLKKFSNKPFVCAK
Sbjct: 601 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 660

Query: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFA TDLNYPSISVPKLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           SVGEEK FKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 761

BLAST of Csor.00g205720 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 609/768 (79.30%), Postives = 677/768 (88.15%), Query Frame = 0

Query: 4   SYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVK 63
           SY+ISPLLLFFF+L T++V T+KSYIVYLGS S  FG NPSIYDVQLATES+YDILG+VK
Sbjct: 8   SYVISPLLLFFFVLQTAAVPTQKSYIVYLGSHS--FGPNPSIYDVQLATESQYDILGSVK 67

Query: 64  GSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLG 123
           GSK+AAK+SILY+YNR INGFAAVLDD E TALAKNPSVVS+FEN+ERKLHTTRSW FLG
Sbjct: 68  GSKVAAKDSILYSYNRYINGFAAVLDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLG 127

Query: 124 VDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKF 183
           VDS RGI +NSIWKA++FGED IIGNLDTGVWPES SF+DAGYGPVP RW GAC+GGSKF
Sbjct: 128 VDSDRGIPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKF 187

Query: 184 RCNRKLIGARYFYRGYEMINGPL---NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 243
           RCNRKLIGARYFYRG++    P    NIS  +A+DHEGHG+HTLSTAGGNFV G NVFGN
Sbjct: 188 RCNRKLIGARYFYRGFQASERPWTKQNISFDSAQDHEGHGSHTLSTAGGNFVHGVNVFGN 247

Query: 244 GNGTAKGGAPKARVAAYKVCWPQ--GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFA 303
           GNGTAKGG+P+ARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS SLG  AQ+FA
Sbjct: 248 GNGTAKGGSPRARVVAYKVCWPSENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFA 307

Query: 304 NDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNK 363
           +D IS+GAFHA+Q GI+VVCSAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNK
Sbjct: 308 HDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNK 367

Query: 364 KQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRG 423
           KQ++GSSLSS  LP GK YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG
Sbjct: 368 KQLRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRG 427

Query: 424 DNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKR 483
           +N R+ K FEV RVGG+GM+LVN++I GS I  DPH+LP SH+S  DG+SI QYL STK 
Sbjct: 428 ENARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKT 487

Query: 484 PVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDL 543
           PVA+IT   TE+GIKPSP+MA+FSSRGP+ ITEA+IKPDITAPGVNIIAS T    A+ L
Sbjct: 488 PVASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGL 547

Query: 544 PFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTML 603
           PFDKRRVPFN++SGTSMSCPHISGVAGLLK LHPTWSP AIKSAIMTTAKTRDNTKNT+L
Sbjct: 548 PFDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTIL 607

Query: 604 DFNKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC 663
           D+ KVKATPFDYGAG VHPNNAMDPGLVYDTT+DDYLNFLCT+GYNS TLKKFSNKPFVC
Sbjct: 608 DYTKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVC 667

Query: 664 AKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQ 723
           AKNFA TD NYPSI VP+LQIG  VTVNRRVKNVGSAGTYVARV+MPKGITVMVEPSTLQ
Sbjct: 668 AKNFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQ 727

Query: 724 FHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766
           FHSVGEEKPFKLVFHYA K+RR GYVFGALVWSDGKHFVRS IAVNLV
Sbjct: 728 FHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNLV 773

BLAST of Csor.00g205720 vs. ExPASy TrEMBL
Match: A0A6J1KYS5 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498344 PE=3 SV=1)

HSP 1 Score: 1228 bits (3176), Expect = 0.0
Identity = 604/766 (78.85%), Postives = 674/766 (87.99%), Query Frame = 0

Query: 7   ISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSK 66
           ISPLLLFFF+LHT++V TK SYIVYLGS S  F  NPS+YDVQLATES+YDILG+VKGSK
Sbjct: 7   ISPLLLFFFMLHTAAVPTKNSYIVYLGSHS--FAPNPSVYDVQLATESQYDILGSVKGSK 66

Query: 67  MAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDS 126
           +AAK+SILY+YNR INGFAAVLD+ E TALAKNPSVVSVFEN+ERKLHTTRSWGFLGVDS
Sbjct: 67  VAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDS 126

Query: 127 GRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFRCN 186
            RGI QNSIWKA++FG D IIGNLDTGVWPES SF+DAGYGPVP RW GACEGGSKFRCN
Sbjct: 127 DRGIPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCN 186

Query: 187 RKLIGARYFYRGYEMINGPL---NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNG 246
           RKLIGARYFYRG++   GPL   NIS  +ARDHEGHG+HTLSTAGGNFV G NVFGNGNG
Sbjct: 187 RKLIGARYFYRGFQAAEGPLTTHNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNG 246

Query: 247 TAKGGAPKARVAAYKVCWPQ--GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDP 306
           TAKGG+P+ARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FAND 
Sbjct: 247 TAKGGSPRARVVAYKVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDA 306

Query: 307 ISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQI 366
           IS+GAFHA+Q GI+VVCSAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKK+ 
Sbjct: 307 ISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRF 366

Query: 367 KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNG 426
           +GSSLSS  LP GK YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N 
Sbjct: 367 RGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENA 426

Query: 427 RMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVA 486
           R+ K FEV RVGGVGM+LVN+++ GS +  DPH+LP SH+SY DG+SIAQYL STK PVA
Sbjct: 427 RVSKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVA 486

Query: 487 TITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFD 546
           +IT   TE+GIKPSP+MA+FSSRGP+ IT+A+IKPDITAPGVNIIAS T   +A+ LP D
Sbjct: 487 SITHASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLD 546

Query: 547 KRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFN 606
           KRRVPFN++SGTSMSCPHISGVAGLLK LHPTWSPAAIKSA+MTTAKTRDNTKNT+LD+ 
Sbjct: 547 KRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTLLDYT 606

Query: 607 KVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAKN 666
           KVKATPFDYGAG VHPN+AMDPGLVYDTT+DDYLNFLCT+GYNS TLKKFSNKPFVCA N
Sbjct: 607 KVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANN 666

Query: 667 FAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHS 726
           FA TD NYPSI VP+LQIG  VTVNRRVKNVGS GTYVARVRMPKGITVMVEPS LQFHS
Sbjct: 667 FATTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPSKLQFHS 726

Query: 727 VGEEKPFKLVFHYAQKVRRPG-YVFGALVWSDGKHFVRSPIAVNLV 766
           VGEE+PFKL+FHYA K+RR G YVFGALVWSDGKHFVRS IAVNLV
Sbjct: 727 VGEERPFKLIFHYAHKLRRQGGYVFGALVWSDGKHFVRSSIAVNLV 770

BLAST of Csor.00g205720 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1104 bits (2856), Expect = 0.0
Identity = 543/742 (73.18%), Postives = 620/742 (83.56%), Query Frame = 0

Query: 27  SYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCINGFAA 86
           SYIVYLG+ S   GSNP+ YD+++ATES+YD+LG+V GSK+AAK++I Y+YN+ INGFAA
Sbjct: 6   SYIVYLGAPS--VGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAA 65

Query: 87  VLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGILQNSIWKASKFGEDVI 146
            LD+ +   LAKNP VVSVFEN+ERKLHTTRSW FLGV+S  GI  NSIW A +FGED I
Sbjct: 66  TLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTI 125

Query: 147 IGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFRCNRKLIGARYFYRGYEMINGPL 206
           IGNLDTGVWPES+SF+DAGYGPVP RW GACEGG+ FRCNRKLIGARYF +G+ M +GPL
Sbjct: 126 IGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPL 185

Query: 207 NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCWPQ--- 266
           NIS   ARD +GHG+HTLSTAGGNFV GANVFG GNGTAKGG+PKARVAAYKVCWP    
Sbjct: 186 NISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSG 245

Query: 267 GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAGND 326
           G C DAD+LAG EAAISDGVDVLS+SLG+  ++FA D +S+GAFHA+QQGI+VVCSAGND
Sbjct: 246 GGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGND 305

Query: 327 GPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNSV 386
           GP PGTV+N+SPWMFTV ASSIDR F SY  LGNKK  KGSS+SS  L  GK YPL+N+V
Sbjct: 306 GPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAV 365

Query: 387 NAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDK 446
           +AKA+NAS+ LAQLC +GSLDP KA+GKIIVCLRG+N R++K F VL+ GGVGMILVN K
Sbjct: 366 DAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGK 425

Query: 447 ISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATFSS 506
             GSG   D H+LP +H+SY DGL++AQY+ STK PVA ITPV+T++GIKPSPVMA FSS
Sbjct: 426 NGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSS 485

Query: 507 RGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHISGV 566
           RGPN ITEA++KPDIT PG++I+AS T    AT  PFD RRVPFNV+SGTSMSCPHISGV
Sbjct: 486 RGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGV 545

Query: 567 AGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAMDP 626
            GLLK L+PTWSPAAIKSAIMTTAKTRDNT  T+ D  K KATPFDYGAG VHPN+AMDP
Sbjct: 546 VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDP 605

Query: 627 GLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAKNFAITDLNYPSISVPKLQIGAPV 686
           GLVYDTTIDDYLNFLC +GYNSLT K F NKPFVCAK+F +TDLNYPSIS+PKLQ GAPV
Sbjct: 606 GLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPV 665

Query: 687 TVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQKVRRPGY 746
           TVNRRVKNVG+ GTYVARV     I V VEPSTLQF+SVGEEK FK+VF Y    +  GY
Sbjct: 666 TVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGY 725

Query: 747 VFGALVWSDGKHFVRSPIAVNL 765
           VFG L+WSDGKH VRSPI VNL
Sbjct: 726 VFGTLIWSDGKHNVRSPIVVNL 745

BLAST of Csor.00g205720 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 804.3 bits (2076), Expect = 8.5e-233
Identity = 408/766 (53.26%), Postives = 531/766 (69.32%), Query Frame = 0

Query: 11  LLFFFLLHTSSVH-----TKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGS 70
           LL   L+H SS H        SY+VY G+ S          D     E+ YD LG+  GS
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMD--RVKETHYDFLGSFTGS 69

Query: 71  KMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVD 130
           +  A ++I Y+Y + INGFAA LD +    ++K+P VVSVF N+  KLHTTRSW FLG++
Sbjct: 70  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 129

Query: 131 SGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEG--GSKF 190
               +  +SIW+ ++FGED II NLDTGVWPES+SF D G GP+P RW G C+    + F
Sbjct: 130 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATF 189

Query: 191 RCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNG 250
            CNRKLIGARYF +GY    G LN S  + RD +GHG+HTLSTA G+FV G ++FG GNG
Sbjct: 190 HCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249

Query: 251 TAKGGAPKARVAAYKVCWPQGK---CSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 310
           TAKGG+P+ARVAAYKVCWP  K   C DAD+LA  +AAI DG DV+S+SLG     F ND
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309

Query: 311 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 370
            +++G+FHA ++ I+VVCSAGN GP   TV+NV+PW  TVGAS++DR F S + LGN K 
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369

Query: 371 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 430
            KG SLSS  LP  K YP+M SVNAKA NAS   AQLC+ GSLDP+K +GKI+VCLRG N
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429

Query: 431 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 490
           GR++K   V   GG+GM+L N  ++G+ +  DPH+LP + ++  D  ++++Y+  TK+P+
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489

Query: 491 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 550
           A ITP RT++G+KP+PVMA+FSS+GP+ +   I+KPDITAPGV++IA++T A + T+  F
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549

Query: 551 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 610
           D RR+ FN  SGTSMSCPHISG+AGLLK  +P+WSPAAI+SAIMTTA   D+    + + 
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609

Query: 611 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 670
             +KATPF +GAG V PN A++PGLVYD  I DYLNFLC+ GYN+  +  FS   F C+ 
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669

Query: 671 -NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 730
              ++ +LNYPSI+VP L   + VTV+R VKNVG    Y  +V  P+G+ V V+P++L F
Sbjct: 670 PKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 729

Query: 731 HSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 766
             VGE+K FK++   ++     GYVFG LVWSD KH VRSPI V L
Sbjct: 730 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Csor.00g205720 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-231
Identity = 418/766 (54.57%), Postives = 514/766 (67.10%), Query Frame = 0

Query: 7   ISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQL--ATESKYDILGTVKG 66
           +S LLL   L  + +   KKSYIVYLGS +      P I    L     S    L +  G
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHL----PQISSAHLDGVAHSHRTFLASFVG 80

Query: 67  SKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGV 126
           S   AKE+I Y+Y R INGFAA+LD+NE   +AK+P VVSVF N+ RKLHTT SW F+ +
Sbjct: 81  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 140

Query: 127 DSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFR 186
                + ++S+W  + +GED II NLDTGVWPES+SF D GYG VP RW G C       
Sbjct: 141 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVP 200

Query: 187 CNRKLIGARYFYRGYEMING-PLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNG 246
           CNRKLIGARYF +GY    G P N S    RDH+GHG+HTLSTA GNFV GANVFG GNG
Sbjct: 201 CNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNG 260

Query: 247 TAKGGAPKARVAAYKVCWPQ---GKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 306
           TA GG+PKARVAAYKVCWP     +C DAD+LA IEAAI DGVDVLS S+G  A D+ +D
Sbjct: 261 TASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 320

Query: 307 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 366
            I++G+FHA++ G+ VVCSAGN GP  GTV+NV+PW+ TVGASS+DR F ++V L N + 
Sbjct: 321 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 380

Query: 367 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 426
            KG+SLS   LP  K+Y L+++ +A  +N +   A LC++GSLDP K +GKI+VCLRGDN
Sbjct: 381 FKGTSLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 440

Query: 427 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 486
            R+DK  +    G  GM+L NDK SG+ I +D H+LP S + Y DG ++  YL STK P 
Sbjct: 441 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 500

Query: 487 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 546
             I      +  KP+P MA+FSSRGPN IT  I+KPDITAPGVNIIA+FT A   TDL  
Sbjct: 501 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 560

Query: 547 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 606
           D RR PFN +SGTSMSCPHISGV GLLK LHP WSPAAI+SAIMTT++TR+N +  M+D 
Sbjct: 561 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 620

Query: 607 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKP-FVCA 666
           +  KA PF YG+G V PN A  PGLVYD T  DYL+FLC  GYN+  ++ F+  P + C 
Sbjct: 621 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 680

Query: 667 KNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQF 726
           +   + D NYPSI+VP L     +TV R++KNVG   TY AR R P G+ V VEP  L F
Sbjct: 681 QGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTF 740

Query: 727 HSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 766
           +  GE K F++       V   GYVFG L W+D  H+VRSPI V L
Sbjct: 741 NKTGEVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Csor.00g205720 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 614.4 bits (1583), Expect = 1.3e-175
Identity = 343/767 (44.72%), Postives = 468/767 (61.02%), Query Frame = 0

Query: 10  LLLFFFLLHTSSVHTKK-SYIVYLGSGS--STFGSNPSIYDVQLATESKYDILGTVKGSK 69
           LLL     H SS  + + +YIV++      S+F  + + YD  L +              
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS-------------- 72

Query: 70  MAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDS 129
           ++    +LYTY   I+GF+  L   E  +L   P V+SV      +LHTTR+  FLG+D 
Sbjct: 73  ISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 132

Query: 130 GRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFR-- 189
                   ++  +    DV++G LDTGVWPES+S+ D G+GP+P  W G CE G+ F   
Sbjct: 133 HTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 192

Query: 190 -CNRKLIGARYFYRGYEMINGPLNIS--SLNARDHEGHGTHTLSTAGGNFVQGANVFGNG 249
            CNRKLIGAR+F RGYE   GP++ S  S + RD +GHGTHT STA G+ V+GA++ G  
Sbjct: 193 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 252

Query: 250 NGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDP 309
           +GTA+G AP+ARVA YKVCW  G C  +D+LA I+ AI+D V+VLS+SLG    D+  D 
Sbjct: 253 SGTARGMAPRARVAVYKVCW-LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDG 312

Query: 310 ISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQI 369
           +++GAF A+++GI+V CSAGN GP   +++NV+PW+ TVGA ++DR F +   LGN K  
Sbjct: 313 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 372

Query: 370 KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNG 429
            G SL  G     KL P + + N  ASNA++G   LC  G+L P K +GKI++C RG N 
Sbjct: 373 TGVSLFKGEALPDKLLPFIYAGN--ASNATNG--NLCMTGTLIPEKVKGKIVMCDRGINA 432

Query: 430 RMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVA 489
           R+ K   V   GGVGMIL N   +G  +  D H+LP + V    G  I  Y+ +   P A
Sbjct: 433 RVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTA 492

Query: 490 TITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFD 549
           +I+ + T +G+KPSPV+A FSSRGPN IT  I+KPD+ APGVNI+A++T AA  T L  D
Sbjct: 493 SISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD 552

Query: 550 KRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFN 609
            RRV FN+ SGTSMSCPH+SG+A LLK +HP WSPAAI+SA+MTTA         +LD  
Sbjct: 553 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 612

Query: 610 KVK-ATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVC-- 669
             K +TPFD+GAG V P  A +PGL+YD T +DYL FLC   Y S  ++  S + + C  
Sbjct: 613 TGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 672

Query: 670 AKNFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRM-PKGITVMVEPSTL 729
           +K++++ DLNYPS +V    +GA     R V +VG AGTY  +V     G+ + VEP+ L
Sbjct: 673 SKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 732

Query: 730 QFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVN 765
            F    E+K + + F            FG++ WSDGKH V SP+A++
Sbjct: 733 NFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Csor.00g205720 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 591.7 bits (1524), Expect = 8.7e-169
Identity = 336/785 (42.80%), Postives = 476/785 (60.64%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGS--STFGSNPSIYDVQLATESKYDI 60
           M  S I+  L L F  +  ++    K++I  +  GS  S F ++   Y  + A ES+   
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESR--- 60

Query: 61  LGTVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRS 120
                         I++ Y+   +GF+AV+  +E   L  +P+V++VFE+R R+LHTTRS
Sbjct: 61  --------------IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 WGFLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACE 180
             FLG+ + +G     +W  S +G DVIIG  DTG+WPE  SF D   GP+P RW G CE
Sbjct: 121 PQFLGLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCE 180

Query: 181 GGSKF---RCNRKLIGARYFYRGYE-MINGPLN--ISSLNARDHEGHGTHTLSTAGGNFV 240
            G++F    CNRK+IGAR+F +G +  + G +N  +  L+ RD +GHGTHT STA G   
Sbjct: 181 SGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHA 240

Query: 241 QGANVFGNGNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLG- 300
             A++ G  +G AKG APKAR+AAYKVCW    C D+D+LA  +AA+ DGVDV+SIS+G 
Sbjct: 241 FKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGG 300

Query: 301 --ATAQDFANDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGF 360
                  +  DPI++G++ A  +GI V  SAGN+GP   +VTN++PW+ TVGAS+IDR F
Sbjct: 301 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 360

Query: 361 FSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKAR 420
            +   LG+  +++G SL +G    G+++P++    +  S+AS     LC E +LDP + R
Sbjct: 361 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVR 420

Query: 421 GKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSI 480
           GKI++C RG + R+ K   V + GGVGMIL N   +G G+  D H++P   V   +G  I
Sbjct: 421 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 480

Query: 481 AQYLKSTKRPVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASF 540
             Y  S   P+A+I    T +GIKP+PV+A+FS RGPN ++  I+KPD+ APGVNI+A++
Sbjct: 481 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 540

Query: 541 TRAAAATDLPFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKT 600
           T A   T LP D R+  FN+ SGTSM+CPH+SG A LLK  HP WSPA I+SA+MTT   
Sbjct: 541 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 600

Query: 601 RDNTKNTMLDFNKVK-ATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTL 660
            DN+  +++D +  K ATP+DYG+G ++   AM+PGLVYD T DDY+ FLC+ GY   T+
Sbjct: 601 VDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI 660

Query: 661 KKFSNKPFVC--AKNFAITDLNYPSISV--PKLQIG-APVTVNRRVKNVGSA-GTYVARV 720
           +  +  P  C   +  +  +LNYPSI+   P  + G    TV R   NVG A   Y AR+
Sbjct: 661 QVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 720

Query: 721 RMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQK---VRRPGYVFGALVWSD-GKHFVR 764
             P+G+TV V+P  L F S  + + + +      +   +   G VFG++ W D GKH VR
Sbjct: 721 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 758

BLAST of Csor.00g205720 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-164
Identity = 333/774 (43.02%), Postives = 452/774 (58.40%), Query Frame = 0

Query: 6   IISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGS 65
           I+S  L+F     T+ + TKK+Y++++   +      P    +Q  +     +       
Sbjct: 15  ILSINLIFLQAETTTQISTKKTYVIHMDKSAMPL---PYTNHLQWYSSKINSVTQHKSQE 74

Query: 66  KMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVD 125
           +      ILYTY    +G AA L   E   L +   VV+V      +LHTTRS  FLG++
Sbjct: 75  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 134

Query: 126 SGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKF-- 185
                    +W       DV++G LDTG+WPESESF+D G  PVP  W GACE G +F  
Sbjct: 135 RQE---SERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLK 194

Query: 186 -RCNRKLIGARYFYRGYEMINGPLN--ISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 245
             CNRK++GAR FYRGYE   G ++  +   + RD +GHGTHT +T  G+ V+GAN+FG 
Sbjct: 195 RNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGF 254

Query: 246 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 305
             GTA+G A KARVAAYKVCW  G C  +D+L+ ++ A++DGV VLSISLG     ++ D
Sbjct: 255 AYGTARGMAQKARVAAYKVCW-VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 314

Query: 306 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 365
            +S+  F A++ G+ V CSAGN GP P ++TNVSPW+ TVGAS++DR F + V +G  + 
Sbjct: 315 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 374

Query: 366 IKGSSLSSGG--LPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRG 425
            KG SL  G   LP+ K YPL   V    + +S      C +G+LD     GKI++C RG
Sbjct: 375 FKGVSLYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRG 434

Query: 426 DNGRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKR 485
              R+ K   V R GG+GM+L N   +G  +  D HMLP   V   +G  I QY  ++K+
Sbjct: 435 VTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK 494

Query: 486 PVATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDL 545
             A++  + T IGIKPSPV+A FSSRGPN ++  I+KPD+ APGVNI+A++T   A + L
Sbjct: 495 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 554

Query: 546 PFDKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTML 605
             D RRV FN+ SGTSMSCPH+SGVA L+K  HP WSPAAIKSA+MTTA   DN    + 
Sbjct: 555 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 614

Query: 606 DFN-KVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLK---KFSNK 665
           D +    ++P+D+GAG + P  A DPGLVYD    +Y  FLCTQ  +   LK   K SN+
Sbjct: 615 DASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 674

Query: 666 --PFVCAKNFAITDLNYPSISV--PKLQIGAPVTVNRRVKNVG-SAGTYVARVRMPKGIT 725
                 AKN    +LNYP+IS   P+      +T+ R V NVG    +Y   V   KG +
Sbjct: 675 TCKHTLAKNPG--NLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGAS 734

Query: 726 VMVEPSTLQFHSVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAV 764
           V V+P TL F S  ++  + + F    +++RP   FG LVW    H VRSP+ +
Sbjct: 735 VTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRP--EFGGLVWKSTTHKVRSPVII 774

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSP51.2e-23153.26Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC53.0e-23054.57Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4627.9e-20750.70Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653511.8e-17444.72Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
O496071.2e-16742.80Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6596783.10.0100.00Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022949775.10.099.22subtilisin-like protease SBT5.3 [Cucurbita moschata][more]
XP_023005962.10.094.65uncharacterized protein LOC111498820 [Cucurbita maxima][more]
KAG6596782.10.081.27Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023540421.10.080.21subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GDR20.099.22subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE... [more]
A0A6J1KUL80.094.65uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820... [more]
A0A6J1GDW20.079.30subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE... [more]
A0A6J1KYS50.078.85subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498344 PE=3... [more]
A0A0A0L6010.073.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G04160.18.5e-23353.26Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.12.1e-23154.57Subtilase family protein [more]
AT5G67360.11.3e-17544.72Subtilase family protein [more]
AT4G34980.18.7e-16942.80subtilisin-like serine protease 2 [more]
AT5G51750.11.3e-16443.02subtilase 1.3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 215..228
score: 38.94
coord: 142..161
score: 30.19
coord: 551..567
score: 52.32
IPR003137PA domainPFAMPF02225PAcoord: 398..468
e-value: 5.9E-6
score: 26.3
NoneNo IPR availableGENE3D3.50.30.30coord: 346..490
e-value: 3.1E-183
score: 611.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 638..766
e-value: 5.6E-35
score: 121.9
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 26..765
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 118..621
score: 31.716833
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 352..483
e-value: 3.88485E-34
score: 124.833
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 26..111
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 142..594
e-value: 1.0E-46
score: 159.9
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 16..114
e-value: 1.9E-21
score: 78.3
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 27..114
e-value: 6.1E-13
score: 49.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 144..634
e-value: 3.1E-183
score: 611.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 113..625
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 666..763
e-value: 8.1E-27
score: 93.3
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 26..765
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 552..562
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 112..587
e-value: 1.13899E-136
score: 404.288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g205720.m01Csor.00g205720.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity