Csor.00g195330 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g195330
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionHXXXD-type acyl-transferase family protein
LocationCsor_Chr01: 1579637 .. 1581552 (+)
RNA-Seq ExpressionCsor.00g195330
SyntenyCsor.00g195330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGAGCTTGAAATTGTGTGCAAACAAACCATCAAGCCCTCGTCTTCAACCCCATCGGAGCTTAGAAATTACCCCCTCTGCTTGCTTGATCAACATGCCCCTTACATGTCTATAATGCCTCTCTATTTCTACGACGGCGGCAACACCGTCGACTCCGGCGGCCTGAAGGACGGCCTCTCAAAGGCCTTGACGGTCTACTACCCTTTTGCCGGCCGCCTCCAAAAGGGTGGCAACTTCATCGACTGCAACGACATGGGCGCCATGTACTTAGAAGGAAAGCTCCGGTGCCCCATGTCGGAATTTATGAACAAGCTGCAAAGCGACGAGGTTTTGAAATTGGTTCGTTTAGAACATGACACGAATGGGAAGGACACAGACCCAACATTCAATCCTTTGTTGTCTGTTCAGTTGTTCCATTTTGAATGTGGAGGAGCAATGGTGTCTGTGGTTTTCTCTCATAAAGTGGCGGATTTGGCGTCATTGGCTCAGTTTGTGAATGATTGGGCTTCTATTGCTCGTAGCTCCGGCGGTGGTATGCTGCCGGAAGTCAGTCCTCTGCTCAATGCGGCTAGTTTTTTCCGGCCGGAATTGGATGCCGGCAGTTACCCATCTGGGGATGAAGGTAAAAAGGTTTGTTTGAAGAGATTGGTGTTTGAGAGCTCGAAGATTGAAGCTCTTAAAGCTATGGTTTCTGAGAAAGTGGAAAATCCAAGTCGTGTTCAGGTCATCACTGCCTTCATCTACAAAGCTGCCCTTTCTGCTAAAATTTCAGTCAAAGGTGAGTTAAAGTTGGTTTAAAGGATTGTCCAAATATTTCATACTTATAAATCCATTACTTATAAACCCAGGATCAATTAGTCATCGTGGGACTCTCCATGCCAACAATTCTTCCCTCAAACAAAGTACACTATAGAGCCTCCTTTGAGGCTTATGGAGCCCTTGAACAACTTCCCCTTAATTGAAGCTCGACACTTGAGTCACTTTTGACTATACTTGAAAATTTTATTAACATAGCTAAGTTAAGGGTATGACTCTGATATCATGTTAGGAACCACAACTCTCCACAATGGTATGATATTGTTCACTTTAAGTATAAACTCTCGTGACATTACTTTTAGTTTCCCAAAAATGTCTCGTACTAAAAGACATAGTATTCTTTACTGATAAACCCATGATCAATCCCTAAATTATAGCCAAGTTCCTAAATTATAGCCAACGGAACTCCCTCCCAACAATCCTCAATAGCTAACGGAACTCCCTCCCAACAATCCTCAACACTAAATAGATACAGTACTAGTTTTGTGAAAGAAAAACGCTTGGAATGCATATTTATTATCTCTCTATACACATTCGATAACTTTTGTGCTAATTTCTTGGACAAAAAAGGCCATTGGCCAACGACGTCGACTCTCCTACAGGTGGTGAACATCCGAGGGCGAATGGAGCCACGATTGGAAGAGAGATTAATAGGGAACATAGTCACATACTTCATTGCATCATCAACCAAGGAGGAGGAGGGGAGGGAAATGGAGGTATGGAAGATAGTGGGAGACATGAAGAAAAGCTTTGAAGACTTCTGCAGGGAGTTCCCAAAGAAGTGCAGGGCGGAAGAATGGAATGGGCTTTACAAATTGCATGCCAAACAGAAAATGGATAGGCTGATAAGTGGTGGATGGGATCATGCTGTGTACTGTTGCAGTAGCTGGTGCAAATTTCCACTATATGATGCAGATTTTGGATGGGGGAAGCCTATCTGGATTACTGTTCCAGATTTTGACTCCAAAAATCTGATTATGTTGATGGATGCAAGAGATGGGAAGGGAGTTGAAGCCATTGTGAGATTGGAGGAGGAGGAAATGGCTGTGTTTCAGCAAAATCAAGAGCTTCTCTCCTTTTGTGACATAAGAACTTAG

mRNA sequence

ATGGTTGAGCTTGAAATTGTGTGCAAACAAACCATCAAGCCCTCGTCTTCAACCCCATCGGAGCTTAGAAATTACCCCCTCTGCTTGCTTGATCAACATGCCCCTTACATGTCTATAATGCCTCTCTATTTCTACGACGGCGGCAACACCGTCGACTCCGGCGGCCTGAAGGACGGCCTCTCAAAGGCCTTGACGGTCTACTACCCTTTTGCCGGCCGCCTCCAAAAGGGTGGCAACTTCATCGACTGCAACGACATGGGCGCCATGTACTTAGAAGGAAAGCTCCGGTGCCCCATGTCGGAATTTATGAACAAGCTGCAAAGCGACGAGGTTTTGAAATTGGTTCGTTTAGAACATGACACGAATGGGAAGGACACAGACCCAACATTCAATCCTTTGTTGTCTGTTCAGTTGTTCCATTTTGAATGTGGAGGAGCAATGGTGTCTGTGGTTTTCTCTCATAAAGTGGCGGATTTGGCGTCATTGGCTCAGTTTGTGAATGATTGGGCTTCTATTGCTCGTAGCTCCGGCGGTGGTATGCTGCCGGAAGTCAGTCCTCTGCTCAATGCGGCTAGTTTTTTCCGGCCGGAATTGGATGCCGGCAGTTACCCATCTGGGGATGAAGGTAAAAAGGTTTGTTTGAAGAGATTGGTGTTTGAGAGCTCGAAGATTGAAGCTCTTAAAGCTATGGTTTCTGAGAAAGTGGAAAATCCAAGTCGTGTTCAGGTCATCACTGCCTTCATCTACAAAGCTGCCCTTTCTGCTAAAATTTCAGTCAAAGGCCATTGGCCAACGACGTCGACTCTCCTACAGGTGGTGAACATCCGAGGGCGAATGGAGCCACGATTGGAAGAGAGATTAATAGGGAACATAGTCACATACTTCATTGCATCATCAACCAAGGAGGAGGAGGGGAGGGAAATGGAGGTATGGAAGATAGTGGGAGACATGAAGAAAAGCTTTGAAGACTTCTGCAGGGAGTTCCCAAAGAAGTGCAGGGCGGAAGAATGGAATGGGCTTTACAAATTGCATGCCAAACAGAAAATGGATAGGCTGATAAGTGGTGGATGGGATCATGCTGTGTACTGTTGCAGTAGCTGGTGCAAATTTCCACTATATGATGCAGATTTTGGATGGGGGAAGCCTATCTGGATTACTGTTCCAGATTTTGACTCCAAAAATCTGATTATGTTGATGGATGCAAGAGATGGGAAGGGAGTTGAAGCCATTGTGAGATTGGAGGAGGAGGAAATGGCTGTGTTTCAGCAAAATCAAGAGCTTCTCTCCTTTTGTGACATAAGAACTTAG

Coding sequence (CDS)

ATGGTTGAGCTTGAAATTGTGTGCAAACAAACCATCAAGCCCTCGTCTTCAACCCCATCGGAGCTTAGAAATTACCCCCTCTGCTTGCTTGATCAACATGCCCCTTACATGTCTATAATGCCTCTCTATTTCTACGACGGCGGCAACACCGTCGACTCCGGCGGCCTGAAGGACGGCCTCTCAAAGGCCTTGACGGTCTACTACCCTTTTGCCGGCCGCCTCCAAAAGGGTGGCAACTTCATCGACTGCAACGACATGGGCGCCATGTACTTAGAAGGAAAGCTCCGGTGCCCCATGTCGGAATTTATGAACAAGCTGCAAAGCGACGAGGTTTTGAAATTGGTTCGTTTAGAACATGACACGAATGGGAAGGACACAGACCCAACATTCAATCCTTTGTTGTCTGTTCAGTTGTTCCATTTTGAATGTGGAGGAGCAATGGTGTCTGTGGTTTTCTCTCATAAAGTGGCGGATTTGGCGTCATTGGCTCAGTTTGTGAATGATTGGGCTTCTATTGCTCGTAGCTCCGGCGGTGGTATGCTGCCGGAAGTCAGTCCTCTGCTCAATGCGGCTAGTTTTTTCCGGCCGGAATTGGATGCCGGCAGTTACCCATCTGGGGATGAAGGTAAAAAGGTTTGTTTGAAGAGATTGGTGTTTGAGAGCTCGAAGATTGAAGCTCTTAAAGCTATGGTTTCTGAGAAAGTGGAAAATCCAAGTCGTGTTCAGGTCATCACTGCCTTCATCTACAAAGCTGCCCTTTCTGCTAAAATTTCAGTCAAAGGCCATTGGCCAACGACGTCGACTCTCCTACAGGTGGTGAACATCCGAGGGCGAATGGAGCCACGATTGGAAGAGAGATTAATAGGGAACATAGTCACATACTTCATTGCATCATCAACCAAGGAGGAGGAGGGGAGGGAAATGGAGGTATGGAAGATAGTGGGAGACATGAAGAAAAGCTTTGAAGACTTCTGCAGGGAGTTCCCAAAGAAGTGCAGGGCGGAAGAATGGAATGGGCTTTACAAATTGCATGCCAAACAGAAAATGGATAGGCTGATAAGTGGTGGATGGGATCATGCTGTGTACTGTTGCAGTAGCTGGTGCAAATTTCCACTATATGATGCAGATTTTGGATGGGGGAAGCCTATCTGGATTACTGTTCCAGATTTTGACTCCAAAAATCTGATTATGTTGATGGATGCAAGAGATGGGAAGGGAGTTGAAGCCATTGTGAGATTGGAGGAGGAGGAAATGGCTGTGTTTCAGCAAAATCAAGAGCTTCTCTCCTTTTGTGACATAAGAACTTAG

Protein sequence

MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAVFQQNQELLSFCDIRT
Homology
BLAST of Csor.00g195330 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.2e-57
Identity = 152/438 (34.70%), Postives = 236/438 (53.88%), Query Frame = 0

Query: 4   LEIVCKQTIKPSSSTPSELRNYPLCLLDQ--HAPYMSIMPLYFYDGGNTVDSGG--LKDG 63
           +EIV K  IKPSS TP  LR Y L  +DQ   +   S+   Y Y+  +     G  LK+ 
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGDLLKNS 65

Query: 64  LSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQS---DEVL--KL 123
           LSK L  YY FAGRL +  ++IDCND G  ++E ++   M++ + + +S   D VL  ++
Sbjct: 66  LSKTLVSYYQFAGRLIE-NDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLPTRI 125

Query: 124 VRLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIAR 183
           + L  D+           LL VQL HF+CGG  +    SHKV+D  S   F+ DWAS   
Sbjct: 126 IALHEDS-----------LLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS--- 185

Query: 184 SSGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCL-KRLVFESSKIEALKAMVSE 243
           S+      + +PLL A S F PE +           + CL KR VF +  IE LK+   E
Sbjct: 186 STSLSTFHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE 245

Query: 244 -KVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 303
             ++ PSRV+V+TAF+ + A +  +  K  +     ++  VN+R  +   L +  IGNI 
Sbjct: 246 YGIQKPSRVEVVTAFLCQCAANCDLPRKKPY----AIISAVNLRPYL--ALPQNSIGNIF 305

Query: 304 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 363
           +++   +   +EG + +   ++  ++   +        K +    + + +L   + +D+L
Sbjct: 306 SFYFCIN---DEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQEL--TKCLDQL 365

Query: 364 ISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVR 423
                D   Y CSSWC+FP+YD DFGWGKPI ++      K+LI+LMD+ +G G+EA++ 
Sbjct: 366 NISSLD--TYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALIT 415

Query: 424 LEEEEMAVFQQNQELLSF 431
           +EE++MA F++N+EL SF
Sbjct: 426 MEEKKMAAFEKNEELRSF 415

BLAST of Csor.00g195330 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.9e-57
Identity = 152/442 (34.39%), Postives = 234/442 (52.94%), Query Frame = 0

Query: 7   VC-KQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFY--------DGGNTVDSGG-- 66
           VC K  IKPSS TPS LR + L  +DQ    M I   +FY        D  N+ +     
Sbjct: 12  VCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 67  --LKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLK 126
             L+  LS+ L  YYP+AG+L K    +DCNDMGA +L  +++C MSE ++   +     
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKL-KDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAES 131

Query: 127 LVRLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIA 186
           +V L  D    +     N LL VQ+  F+CGG  +SV FSHK+ D  SL  F+NDW+S+ 
Sbjct: 132 IV-LPKDLPWANNCEGGN-LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 191

Query: 187 RSSGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCL-KRLVFESSKIEALKAMVS 246
           R      L      +  + F      + + P        C+ KRL+F + K++AL+A V+
Sbjct: 192 RDHTTTTLVPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAKVA 251

Query: 247 EK--VENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGN 306
           E+  V+NP+R +V++A ++K A  A  S+       S L+  +NIR  ++PRL    IGN
Sbjct: 252 EESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNTIGN 311

Query: 307 IVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMD 366
           + + F   +T  +   +ME+  +V +++K  E       KK + E+   + ++    +  
Sbjct: 312 LSSIFSIEATNMQ---DMELPTLVRNLRKEVE----VAYKKDQVEQNELILEVVESMREG 371

Query: 367 RLISGGWD--HAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVE 426
           +L     D    VY CS+ CK+P Y  DFGWG+P  + + +  SKN   L D + G+GVE
Sbjct: 372 KLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVE 431

Query: 427 AIVRLEEEEMAVFQQNQELLSF 431
           A V L +++M+ F++N+ELL F
Sbjct: 432 ARVMLHKQQMSEFERNEELLEF 438

BLAST of Csor.00g195330 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.8e-57
Identity = 151/444 (34.01%), Postives = 234/444 (52.70%), Query Frame = 0

Query: 4   LEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFY--------DGGNTVDSGG 63
           + I  K  IKPSS TPS LR + L  +DQ    M I   +FY        D  N+ +   
Sbjct: 10  VSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 64  ----LKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEV 123
               L+  LS+ L  YYP+AG+L K    +DCNDMGA +L  +++C MSE ++   +   
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKL-KDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLA 129

Query: 124 LKLVRLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWAS 183
             +V L  D    +     N LL VQ+  F+CGG  +SV FSHK+ D  SL  F+NDW+S
Sbjct: 130 ESIV-LPKDLPWANNCEGGN-LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 189

Query: 184 IARSSGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCL-KRLVFESSKIEALKAM 243
           + R      L      +  + F   +  +   P        C+ KRL+F + K++AL+A 
Sbjct: 190 VTRDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDALRAK 249

Query: 244 VSEK--VENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLI 303
           V+E+  V+NP+R +V++A ++K A  A  S+       S L+  +NIR  ++PRL    I
Sbjct: 250 VAEESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNAI 309

Query: 304 GNIVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQK 363
           GN+ + F   +T  +   +ME+  +V +++K  E       KK + E+   + ++    +
Sbjct: 310 GNLSSIFSIEATNMQ---DMELPTLVRNLRKEVE----VAYKKDQVEQNELILEVVESMR 369

Query: 364 MDRLISGGWD--HAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKG 423
             +L     D    VY CS+ CK+P Y  DFGWG+P  + + +  SKN   L D + G+G
Sbjct: 370 EGKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQG 429

Query: 424 VEAIVRLEEEEMAVFQQNQELLSF 431
           VEA V L +++M+ F++N+ELL F
Sbjct: 430 VEARVMLHKQQMSEFERNEELLEF 438

BLAST of Csor.00g195330 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.0e-57
Identity = 152/442 (34.39%), Postives = 234/442 (52.94%), Query Frame = 0

Query: 7   VC-KQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFY--------DGGNTVDSGG-- 66
           VC K  IKPSS TPS LR + L  +DQ    M I   +FY        D  N+ +     
Sbjct: 12  VCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 67  --LKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLK 126
             L+  LS+ L  YYP+AG+L K    +DCNDMGA +L  +++C MSE ++   +     
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKL-KDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAES 131

Query: 127 LVRLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIA 186
           +V L  D    +     N LL VQ+  F+CGG  +SV FSHK+ D  SL  F+NDW+S+ 
Sbjct: 132 IV-LPKDLPWANNCEGGN-LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 191

Query: 187 RSSGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCL-KRLVFESSKIEALKAMVS 246
           R      L      +  + F   +  +   P        C+ KRL+F + K++AL+A V+
Sbjct: 192 RDRTTTTLVPSPRFVGDSVFSTQKYGSLITPQILSDLNQCVQKRLIFPTDKLDALRAKVA 251

Query: 247 EK--VENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGN 306
           E+  V+NP+R +V++A ++K A  A  S+       S L+  +NIR  ++PRL    IGN
Sbjct: 252 EESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNAIGN 311

Query: 307 IVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMD 366
           + + F   +T  +   +ME+  +V +++K  E       KK + E+   + ++    +  
Sbjct: 312 LSSIFSIEATNMQ---DMELPTLVRNLRKEVE----VAYKKDQVEQNELILEVVESMREG 371

Query: 367 RLISGGWD--HAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVE 426
           +L     D    VY CS+ CK+P Y  DFGWG+P  + + +  SKN   L D + G+GVE
Sbjct: 372 KLPFENMDGYKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVE 431

Query: 427 AIVRLEEEEMAVFQQNQELLSF 431
           A V L +++M+ F++N+ELL F
Sbjct: 432 ARVMLHKQQMSEFERNEELLEF 438

BLAST of Csor.00g195330 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 8.8e-54
Identity = 149/445 (33.48%), Postives = 230/445 (51.69%), Query Frame = 0

Query: 2   VELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSG----GLK 61
           VE E + K  IKPSS TP  L  Y L   DQ+     +   +FY+  + ++       L+
Sbjct: 8   VETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYENPDGIEISTIREQLQ 67

Query: 62  DGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRL 121
           + LSK L  YYPFAG++ K  ++I CND G  ++E ++RC M++ +          LV  
Sbjct: 68  NSLSKTLVSYYPFAGKVVK-NDYIHCNDDGIEFVEVRIRCRMNDILKYELRSYARDLVLP 127

Query: 122 EHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDW-ASIARSS 181
           +  T G +          VQL HF+CGG  V+   SHKVAD  ++A F+ DW AS    S
Sbjct: 128 KRVTVGSEDTTAI-----VQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAASACYLS 187

Query: 182 GGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSE-KV 241
               +P  +PLL + S F P  D          K    K  +F    IE LK+   E  +
Sbjct: 188 SSHHVP--TPLLVSDSIF-PRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVEFGI 247

Query: 242 ENPSRVQVITAFIYK-AALSAKISVKGHWPTTS---TLLQVVNIRGRMEPRLEERLIGNI 301
           E P+RV+V+TAF+ + A ++ K + K +    S    +LQ +N+R  +E  L +  +GN+
Sbjct: 248 EKPTRVEVLTAFLSRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPILE--LPQNSVGNL 307

Query: 302 VTYFIASSTKEEEG-REMEVWK-IVGDMKKSFEDFCREFPKKCRAEEWNGLYKLH--AKQ 361
           V+ + + + KE +   E E  K ++ +++K      ++  K    E+   + ++    K 
Sbjct: 308 VSIYFSRTIKENDYLNEKEYTKLVINELRKE-----KQKIKNLSREKLTYVAQMEEFVKS 367

Query: 362 KMDRLISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMD--ARDGK 421
             +  IS   D   Y   SWC+FP YD DFGWGKPIW+ +     KN +++MD    D  
Sbjct: 368 LKEFDISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYPFGDDY 427

Query: 422 GVEAIVRLEEEEMAVFQQNQELLSF 431
           G+EAIV  E+E+M+ F++N++LL F
Sbjct: 428 GIEAIVSFEQEKMSAFEKNEQLLQF 436

BLAST of Csor.00g195330 vs. NCBI nr
Match: KAG6606973.1 (Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL
Sbjct: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD
Sbjct: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR
Sbjct: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST
Sbjct: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA
Sbjct: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           FQQNQELLSFCDIRT
Sbjct: 421 FQQNQELLSFCDIRT 435

BLAST of Csor.00g195330 vs. NCBI nr
Match: XP_022948443.1 (deacetylvindoline O-acetyltransferase-like [Cucurbita moschata])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 431/435 (99.08%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL
Sbjct: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALT+YYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD
Sbjct: 61  SKALTIYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR
Sbjct: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST
Sbjct: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMK+SFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA
Sbjct: 301 KEEEGREMEVWKIVGDMKRSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWCKFPLYDADFGWGKP+WITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCKFPLYDADFGWGKPMWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           FQQNQELLSFCD+RT
Sbjct: 421 FQQNQELLSFCDLRT 435

BLAST of Csor.00g195330 vs. NCBI nr
Match: XP_023524683.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847 bits (2189), Expect = 1.13e-309
Identity = 411/435 (94.48%), Postives = 423/435 (97.24%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCK+TIKPSSSTPSELRNYPLCLLDQHAP +SIM LYFYDGGNTVDSGGLKDGL
Sbjct: 1   MVELEIVCKETIKPSSSTPSELRNYPLCLLDQHAPCVSIMLLYFYDGGNTVDSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQ DEVLKLVRLEHD
Sbjct: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQRDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDP FNPLL VQLFHFECGGAMVSVVFSHKVADLASL QFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPRFNPLLCVQLFHFECGGAMVSVVFSHKVADLASLTQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           LPEVSPL NAASFFRPELDAGSYPS   GKKVCLKRLVFESSKIEALKAMVSEKVENPSR
Sbjct: 181 LPEVSPLFNAASFFRPELDAGSYPSEVGGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQV+TAFIYKAALSAK+SVKGHWPTTSTLLQVVNIRGRMEPRLEE+LIGNIV++FIASST
Sbjct: 241 VQVLTAFIYKAALSAKVSVKGHWPTTSTLLQVVNIRGRMEPRLEEKLIGNIVSFFIASST 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMK+S EDFCREFPKKC AEEWNGLYKLHAK+KMDRLISGGWDHA
Sbjct: 301 KEEEGREMEVWKIVGDMKRSLEDFCREFPKKCSAEEWNGLYKLHAKEKMDRLISGGWDHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWCKFPLYDADFGWGKP+WITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCKFPLYDADFGWGKPMWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           F+QNQELLSFCD+RT
Sbjct: 421 FEQNQELLSFCDLRT 435

BLAST of Csor.00g195330 vs. NCBI nr
Match: XP_022997739.1 (vinorine synthase-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 832 bits (2148), Expect = 2.00e-303
Identity = 402/435 (92.41%), Postives = 419/435 (96.32%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCK+TIKPSSSTPSELRNYPLCLLDQHAPYMSIM LYFYDGGNTV SGGLKDGL
Sbjct: 1   MVELEIVCKETIKPSSSTPSELRNYPLCLLDQHAPYMSIMLLYFYDGGNTVHSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQ DEVLKLVRLEHD
Sbjct: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQRDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDPT NPLLSVQLFHFECGG M+SVVFSHKVADLASLAQFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPTLNPLLSVQLFHFECGGTMMSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           L EV+PL NAASFFRPELDAG YPS +EGKKVCLKRLVFE SKIEALKAMVSEKVENPSR
Sbjct: 181 LLEVNPLFNAASFFRPELDAGGYPSVEEGKKVCLKRLVFEGSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQV+TAF+YKAALSAKISVKGHWPT S+LLQVVNIRGRMEP LEE+LIGNIVT+FIASS 
Sbjct: 241 VQVLTAFVYKAALSAKISVKGHWPTMSSLLQVVNIRGRMEPPLEEKLIGNIVTFFIASSA 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMK+S E+FCRE PKKCRAEEWNGLYKLHAKQKMDRLISGGW+HA
Sbjct: 301 KEEEGREMEVWKIVGDMKRSLEEFCRELPKKCRAEEWNGLYKLHAKQKMDRLISGGWNHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWC+FPLYDADFGWGKP+WITVPDFDSKNLI+LMDARDG+GVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCRFPLYDADFGWGKPMWITVPDFDSKNLILLMDARDGEGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           FQQNQELLSFCD+ T
Sbjct: 421 FQQNQELLSFCDLIT 435

BLAST of Csor.00g195330 vs. NCBI nr
Match: XP_022997736.1 (vinorine synthase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 779 bits (2011), Expect = 2.03e-282
Identity = 382/443 (86.23%), Postives = 404/443 (91.20%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELE+VCK+TIKP SSTPSELRN+PLCL DQ AP + IM LYFYD GNTVDSGGLKD L
Sbjct: 1   MVELEMVCKETIKPPSSTPSELRNHPLCLFDQQAPDLYIMLLYFYDNGNTVDSGGLKDSL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMN-KLQSDEVLKLVRLEH 120
           SKALTVYYPFAGRLQKGGN IDCNDMGAMY EGKLRCPMSE MN KLQ D++LKLV L+H
Sbjct: 61  SKALTVYYPFAGRLQKGGNSIDCNDMGAMYSEGKLRCPMSEVMNNKLQHDQILKLVCLDH 120

Query: 121 DTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGG 180
           DTNG DTDP FNPLLSVQLFHFECGGAM+SV   HKVADLASL  FVNDWASIARSSGGG
Sbjct: 121 DTNGNDTDPRFNPLLSVQLFHFECGGAMMSVSCYHKVADLASLTNFVNDWASIARSSGGG 180

Query: 181 MLPEVSPLLNAASFFRPELDAGSYPSGDEG-------KKVCLKRLVFESSKIEALKAMVS 240
           MLP V+PL NAASFFRPELDAG  PSGDEG       KKVCLKRLVFE SKIEALKAMVS
Sbjct: 181 MLPAVTPLFNAASFFRPELDAGGNPSGDEGSEAKGGGKKVCLKRLVFEGSKIEALKAMVS 240

Query: 241 EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 300
           EKVENPSRVQV+TAF+YKAALSAKISVKGHWPT S+LLQVVNIRGRMEP LEE+LIGNIV
Sbjct: 241 EKVENPSRVQVLTAFVYKAALSAKISVKGHWPTMSSLLQVVNIRGRMEPPLEEKLIGNIV 300

Query: 301 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 360
           T+FIASS KEEEGREMEVWKIVGDMK+S E+FCRE PKKCRAEEWNGLYKLHAKQKMDRL
Sbjct: 301 TFFIASSAKEEEGREMEVWKIVGDMKRSLEEFCRELPKKCRAEEWNGLYKLHAKQKMDRL 360

Query: 361 ISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVR 420
           ISGGW+HAVYCCSSWC+FPLYDADFGWGKP+WITVPDFDSKNLI+LMDARDG+GVEAIVR
Sbjct: 361 ISGGWNHAVYCCSSWCRFPLYDADFGWGKPMWITVPDFDSKNLILLMDARDGEGVEAIVR 420

Query: 421 LEEEEMAVFQQNQELLSFCDIRT 435
           LEEEEMAVFQQNQELLSFCD+ T
Sbjct: 421 LEEEEMAVFQQNQELLSFCDLIT 443

BLAST of Csor.00g195330 vs. ExPASy TrEMBL
Match: A0A6J1G9B6 (deacetylvindoline O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111452125 PE=3 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 431/435 (99.08%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL
Sbjct: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALT+YYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD
Sbjct: 61  SKALTIYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR
Sbjct: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST
Sbjct: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMK+SFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA
Sbjct: 301 KEEEGREMEVWKIVGDMKRSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWCKFPLYDADFGWGKP+WITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCKFPLYDADFGWGKPMWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           FQQNQELLSFCD+RT
Sbjct: 421 FQQNQELLSFCDLRT 435

BLAST of Csor.00g195330 vs. ExPASy TrEMBL
Match: A0A6J1KCE9 (vinorine synthase-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE=3 SV=1)

HSP 1 Score: 832 bits (2148), Expect = 9.66e-304
Identity = 402/435 (92.41%), Postives = 419/435 (96.32%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCK+TIKPSSSTPSELRNYPLCLLDQHAPYMSIM LYFYDGGNTV SGGLKDGL
Sbjct: 1   MVELEIVCKETIKPSSSTPSELRNYPLCLLDQHAPYMSIMLLYFYDGGNTVHSGGLKDGL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRLEHD 120
           SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQ DEVLKLVRLEHD
Sbjct: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQRDEVLKLVRLEHD 120

Query: 121 TNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180
           TNGKDTDPT NPLLSVQLFHFECGG M+SVVFSHKVADLASLAQFVNDWASIARSSGGGM
Sbjct: 121 TNGKDTDPTLNPLLSVQLFHFECGGTMMSVVFSHKVADLASLAQFVNDWASIARSSGGGM 180

Query: 181 LPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAMVSEKVENPSR 240
           L EV+PL NAASFFRPELDAG YPS +EGKKVCLKRLVFE SKIEALKAMVSEKVENPSR
Sbjct: 181 LLEVNPLFNAASFFRPELDAGGYPSVEEGKKVCLKRLVFEGSKIEALKAMVSEKVENPSR 240

Query: 241 VQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIVTYFIASST 300
           VQV+TAF+YKAALSAKISVKGHWPT S+LLQVVNIRGRMEP LEE+LIGNIVT+FIASS 
Sbjct: 241 VQVLTAFVYKAALSAKISVKGHWPTMSSLLQVVNIRGRMEPPLEEKLIGNIVTFFIASSA 300

Query: 301 KEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRLISGGWDHA 360
           KEEEGREMEVWKIVGDMK+S E+FCRE PKKCRAEEWNGLYKLHAKQKMDRLISGGW+HA
Sbjct: 301 KEEEGREMEVWKIVGDMKRSLEEFCRELPKKCRAEEWNGLYKLHAKQKMDRLISGGWNHA 360

Query: 361 VYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVRLEEEEMAV 420
           VYCCSSWC+FPLYDADFGWGKP+WITVPDFDSKNLI+LMDARDG+GVEAIVRLEEEEMAV
Sbjct: 361 VYCCSSWCRFPLYDADFGWGKPMWITVPDFDSKNLILLMDARDGEGVEAIVRLEEEEMAV 420

Query: 421 FQQNQELLSFCDIRT 435
           FQQNQELLSFCD+ T
Sbjct: 421 FQQNQELLSFCDLIT 435

BLAST of Csor.00g195330 vs. ExPASy TrEMBL
Match: A0A6J1K8B2 (vinorine synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE=3 SV=1)

HSP 1 Score: 779 bits (2011), Expect = 9.81e-283
Identity = 382/443 (86.23%), Postives = 404/443 (91.20%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELE+VCK+TIKP SSTPSELRN+PLCL DQ AP + IM LYFYD GNTVDSGGLKD L
Sbjct: 1   MVELEMVCKETIKPPSSTPSELRNHPLCLFDQQAPDLYIMLLYFYDNGNTVDSGGLKDSL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMN-KLQSDEVLKLVRLEH 120
           SKALTVYYPFAGRLQKGGN IDCNDMGAMY EGKLRCPMSE MN KLQ D++LKLV L+H
Sbjct: 61  SKALTVYYPFAGRLQKGGNSIDCNDMGAMYSEGKLRCPMSEVMNNKLQHDQILKLVCLDH 120

Query: 121 DTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGG 180
           DTNG DTDP FNPLLSVQLFHFECGGAM+SV   HKVADLASL  FVNDWASIARSSGGG
Sbjct: 121 DTNGNDTDPRFNPLLSVQLFHFECGGAMMSVSCYHKVADLASLTNFVNDWASIARSSGGG 180

Query: 181 MLPEVSPLLNAASFFRPELDAGSYPSGDEG-------KKVCLKRLVFESSKIEALKAMVS 240
           MLP V+PL NAASFFRPELDAG  PSGDEG       KKVCLKRLVFE SKIEALKAMVS
Sbjct: 181 MLPAVTPLFNAASFFRPELDAGGNPSGDEGSEAKGGGKKVCLKRLVFEGSKIEALKAMVS 240

Query: 241 EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 300
           EKVENPSRVQV+TAF+YKAALSAKISVKGHWPT S+LLQVVNIRGRMEP LEE+LIGNIV
Sbjct: 241 EKVENPSRVQVLTAFVYKAALSAKISVKGHWPTMSSLLQVVNIRGRMEPPLEEKLIGNIV 300

Query: 301 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 360
           T+FIASS KEEEGREMEVWKIVGDMK+S E+FCRE PKKCRAEEWNGLYKLHAKQKMDRL
Sbjct: 301 TFFIASSAKEEEGREMEVWKIVGDMKRSLEEFCRELPKKCRAEEWNGLYKLHAKQKMDRL 360

Query: 361 ISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVR 420
           ISGGW+HAVYCCSSWC+FPLYDADFGWGKP+WITVPDFDSKNLI+LMDARDG+GVEAIVR
Sbjct: 361 ISGGWNHAVYCCSSWCRFPLYDADFGWGKPMWITVPDFDSKNLILLMDARDGEGVEAIVR 420

Query: 421 LEEEEMAVFQQNQELLSFCDIRT 435
           LEEEEMAVFQQNQELLSFCD+ T
Sbjct: 421 LEEEEMAVFQQNQELLSFCDLIT 443

BLAST of Csor.00g195330 vs. ExPASy TrEMBL
Match: A0A6J1KES4 (vinorine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE=3 SV=1)

HSP 1 Score: 724 bits (1869), Expect = 3.87e-261
Identity = 359/443 (81.04%), Postives = 391/443 (88.26%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELE+VCK+TIKP SSTPSELRN+PLCL DQ AP + IM LYFYD GNTVDSGGLKD L
Sbjct: 1   MVELEMVCKETIKPPSSTPSELRNHPLCLFDQQAPDLYIMLLYFYDNGNTVDSGGLKDSL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMN-KLQSDEVLKLVRLEH 120
           SKALTVYYPFAGRLQKGGN IDCNDMGAMY EGKLRCPMSE MN KLQ D++LKLV L+H
Sbjct: 61  SKALTVYYPFAGRLQKGGNSIDCNDMGAMYSEGKLRCPMSEVMNNKLQHDQILKLVCLDH 120

Query: 121 DTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGG 180
           DTNG DTDP FNPLLSVQLFHFECGGAM+SV   HKVADLASL  FVNDWASIARSSGGG
Sbjct: 121 DTNGNDTDPRFNPLLSVQLFHFECGGAMMSVSCYHKVADLASLTNFVNDWASIARSSGGG 180

Query: 181 MLPEVSPLLNAASFFRPELDAGSYPSGDEG-------KKVCLKRLVFESSKIEALKAMVS 240
           MLP V+PL NAASFFRPELDAG  PSGDEG       KKVC+KRLVFE SKIEALKAMVS
Sbjct: 181 MLPAVTPLFNAASFFRPELDAGGNPSGDEGSEAKGGGKKVCMKRLVFEGSKIEALKAMVS 240

Query: 241 EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 300
           EKVENP+RVQ++TAFIYKAALSAKISV G+WPTTS LLQ VNIRGRMEP L E+LIGNI+
Sbjct: 241 EKVENPTRVQIVTAFIYKAALSAKISVTGNWPTTS-LLQAVNIRGRMEPALGEKLIGNIL 300

Query: 301 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 360
           ++FIASST EE GREME+W +V DMK+SFE+FCREFPK CRAEEW+ LYKLHAK+KM+RL
Sbjct: 301 SFFIASSTSEE-GREMELWNLVVDMKRSFEEFCREFPKNCRAEEWSWLYKLHAKEKMERL 360

Query: 361 ISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVR 420
            S G DH VY CSSWC FPLY+ DFGWGKPIWITVPDFD+KNLI+LMDARDG+GVEA+V 
Sbjct: 361 GSAGGDHVVYHCSSWCNFPLYEGDFGWGKPIWITVPDFDTKNLIILMDARDGEGVEAMVS 420

Query: 421 LEEEEMAVFQQNQELLSFCDIRT 435
           LEEEEMAVFQQNQ+LLSFCD+RT
Sbjct: 421 LEEEEMAVFQQNQQLLSFCDLRT 441

BLAST of Csor.00g195330 vs. ExPASy TrEMBL
Match: A0A6J1G9W8 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111452124 PE=3 SV=1)

HSP 1 Score: 716 bits (1848), Expect = 6.10e-258
Identity = 357/443 (80.59%), Postives = 388/443 (87.58%), Query Frame = 0

Query: 1   MVELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGGLKDGL 60
           MVELEIVCK+TIKP SSTPSELRN+PLCL+DQ AP   IM LYFYD  NTVD G LKD L
Sbjct: 1   MVELEIVCKETIKPPSSTPSELRNHPLCLIDQQAPNHYIMLLYFYDNRNTVDPGRLKDSL 60

Query: 61  SKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMN-KLQSDEVLKLVRLEH 120
           SKALTVYYPFAGRLQKGGN IDCNDMGAMY EGKLRCPMSE MN KLQ D++LKLV L+H
Sbjct: 61  SKALTVYYPFAGRLQKGGNSIDCNDMGAMYSEGKLRCPMSEVMNNKLQLDQILKLVCLDH 120

Query: 121 DTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSGGG 180
           DTNG DTDP FNPLLSVQLFHFECGGAM+S    HKVADLASL  FVNDWASIARSSGG 
Sbjct: 121 DTNGNDTDPRFNPLLSVQLFHFECGGAMMSASCYHKVADLASLTNFVNDWASIARSSGGD 180

Query: 181 MLPEVSPLLNAASFFRPELDAGSYPSGDEG-------KKVCLKRLVFESSKIEALKAMVS 240
           MLP V+PL NAASFFRPELDAG  PSGDEG       KKV LKRLVFE SKIEALKAMVS
Sbjct: 181 MLPAVTPLFNAASFFRPELDAGGNPSGDEGSEAKGGGKKVFLKRLVFEGSKIEALKAMVS 240

Query: 241 EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 300
           EKV NP+RVQ++TAFIYKAALSAKI V G+WPTTS LLQ VNIR RMEP LEE+LIGNI+
Sbjct: 241 EKVANPTRVQILTAFIYKAALSAKILVTGNWPTTS-LLQTVNIRSRMEPPLEEKLIGNIL 300

Query: 301 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 360
           ++FIASST EE GREME+W +VGDMK+SFE+FCREFPK CRAEEW+ LYKLHAK+KM+RL
Sbjct: 301 SFFIASSTTEE-GREMEIWDLVGDMKRSFEEFCREFPKNCRAEEWSWLYKLHAKEKMERL 360

Query: 361 ISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEAIVR 420
            SGG DH VYCCSSWCKFPLY+ADFGWGKPIWITVPDFD+KNLI+LMDARDG+GVEA+V 
Sbjct: 361 GSGGGDHVVYCCSSWCKFPLYEADFGWGKPIWITVPDFDTKNLIILMDARDGEGVEAMVS 420

Query: 421 LEEEEMAVFQQNQELLSFCDIRT 435
           LEEEEMAVF+QNQELLSFC++RT
Sbjct: 421 LEEEEMAVFEQNQELLSFCELRT 441

BLAST of Csor.00g195330 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 251.9 bits (642), Expect = 9.3e-67
Identity = 154/444 (34.68%), Postives = 243/444 (54.73%), Query Frame = 0

Query: 2   VELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVDSGG----LK 61
           + +++V +  IKPSS TP+ L+ + L LL+Q  P +    ++FY   N++        LK
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLK 60

Query: 62  DGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLVRL 121
             LS+ LT +YP AGRL KG   IDCND GA +LE ++  P+S  + +  SD + +L+  
Sbjct: 61  KSLSETLTHFYPLAGRL-KGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLI-- 120

Query: 122 EHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSSG 181
                  D+  T   LL  Q   FECG   + V  SHK+AD  S+  F+  WA+I  SS 
Sbjct: 121 ---PTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAI--SSR 180

Query: 182 GGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKVCL-----KRLVFESSKIEALKAMVS 241
           G +    +P+ +    F P   + + P+     ++ +     KR +F+SS I+AL+A  S
Sbjct: 181 GSIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKAS 240

Query: 242 E-KVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNI 301
             +V  P+RV+ ++A I+K+A+ A  +V G     S L   V++R R+ P   +  IGN+
Sbjct: 241 SFEVNQPTRVEAVSALIWKSAMKATRTVSG-TSKPSILANSVSLRSRVSPPFTKNSIGNL 300

Query: 302 VTYFIASSTKEEEG-REMEVWKIVGDMKKSFEDFCR-EFPKKCRAEEWNGLYKLHAKQKM 361
           V+YF A   K EEG  + ++  +V  ++K+ + F     PK         +   + K+  
Sbjct: 301 VSYFAA---KAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAG 360

Query: 362 DRLISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKGVEA 421
           D + SG +D   Y  SS C+F LY+ DFGWGKP+W+  P    KN++ L+D ++  G+EA
Sbjct: 361 DMIASGDFD--FYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEA 420

Query: 422 IVRLEEEEMAVFQQNQELLSFCDI 434
            V L E+EM +F+Q++ELL F  +
Sbjct: 421 WVNLNEQEMNLFEQDRELLQFASL 430

BLAST of Csor.00g195330 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 147/450 (32.67%), Postives = 243/450 (54.00%), Query Frame = 0

Query: 2   VELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTV----DSGGLK 61
           +++E + K+ IKPSS+TP++L+   L + D   P +  +   FY   + +     S  LK
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLK 63

Query: 62  DGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKL-RCPMSEFMNKLQSDEVLKLVR 121
             L++ LT +YP AGR++  G  IDCND GA++++ ++   P+S+F+       + +L+ 
Sbjct: 64  TSLAETLTKFYPLAGRIK--GVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLP 123

Query: 122 LEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSS 181
           L+   +  +   T+ PLL V+  +F CGG  + +  +HK+AD  S++ F+  WA++AR  
Sbjct: 124 LDVIDDPYEAATTW-PLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGE 183

Query: 182 GGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKV--CLKRLVFESSKIEAL--KAMVS 241
            G ++ +  P+  AA+F+ P  +   +P  +   K+    KR VF +SK+E L  KA   
Sbjct: 184 AGDIVAD--PVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASE 243

Query: 242 EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNIV 301
           + V  P RV+ +TA ++KA ++A  S   +      L+Q  N+R ++   L E LIGNI+
Sbjct: 244 DFVGRPKRVESVTALLWKAFVAA-ASSNTNACDMKVLIQPSNLRPKIPSLLAESLIGNIM 303

Query: 302 TYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDRL 361
              +     ++E  E+++ K V D++K  +D           +E  G        K+  L
Sbjct: 304 FSSVVLGISQQE--EIKIEKAVRDLRKQGDDLQNVI------KEEGGSSSAMIGSKLANL 363

Query: 362 ISGGWD------HAVYCCSSWCKFPLYDADFGWGKPIWI---TVPDFDSKNLIMLMDARD 421
           +   +       H  Y  SSWCK PLY+A FGWG P+WI     P  +  N+ ML+D++D
Sbjct: 364 MLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLE--NVTMLIDSKD 423

Query: 422 GKGVEAIVRLEEEEMAVFQQNQELLSFCDI 434
           G+G+EA V L EE M   +QN ELL+F  +
Sbjct: 424 GQGIEAFVTLPEENMLSLEQNTELLAFASV 437

BLAST of Csor.00g195330 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-62
Identity = 151/447 (33.78%), Postives = 245/447 (54.81%), Query Frame = 0

Query: 2   VELEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYDGGNTVD----SGGLK 61
           +++E + K+ IKPSS TP+ L+   L + D   P +  +   FY   + +     S  LK
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHKLK 65

Query: 62  DGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKL-RCPMSEFMNKLQSDEVLKLVR 121
             LS+ LT +YP AGR+   G  +DC D GA++++ ++  CP++EF+     D + +L+ 
Sbjct: 66  TSLSETLTKFYPLAGRIT--GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLP 125

Query: 122 LEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARSS 181
           L+   N      T+ PLL V+  +F CGG  + +  +HK+AD AS++ F+  WA+ AR  
Sbjct: 126 LDVVDNPYVAAATW-PLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARGE 185

Query: 182 GGGMLPEVSPLLNAASFFRPELDAGSYPSGDE-GKKVCL-KRLVFESSKIEAL--KAMVS 241
                 E SP+   A+F+ P  +A   P+ ++ GK+  + KR VFE+SK+E L  KA   
Sbjct: 186 NDAAAME-SPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAASE 245

Query: 242 EKVENPSRVQVITAFIYKA-ALSAKISVKGHWPTTSTLLQVVNIRGRMEPRLEERLIGNI 301
           E V+ P+RV+ +TA I+K    S+K +   H      L+Q+ N+R ++   L+E  IGN+
Sbjct: 246 ETVDQPTRVESVTALIWKCFVASSKTTTCDH----KVLVQLANLRSKIPSLLQESSIGNL 305

Query: 302 VTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAKQKMDR 361
           +   +  S     G E+++ + V D++K  E+       +  + + + +       K+  
Sbjct: 306 MFSSVVLSI--GRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSM----IGSKLAN 365

Query: 362 LISGGWD------HAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDS--KNLIMLMDARD 421
           L+   +       H  Y  SSWCK PLY+A FGW  P+W+ V +      NL ML+D++D
Sbjct: 366 LMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWV-VGNVSPVLGNLAMLIDSKD 425

Query: 422 GKGVEAIVRLEEEEMAVFQQNQELLSF 431
           G+G+EA V L EE M+ F+QN ELL+F
Sbjct: 426 GQGIEAFVTLPEENMSSFEQNPELLAF 437

BLAST of Csor.00g195330 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.2e-60
Identity = 151/444 (34.01%), Postives = 249/444 (56.08%), Query Frame = 0

Query: 4   LEIVCKQTIKPSSSTPSELRNYPLCLLDQHAPYMSIMPLYFYD-------GGNTVDSG-G 63
           +EI+ ++ +KPSS TP + R   L LLD  +  M    L FY        G +T ++   
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLK 64

Query: 64  LKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLKLV 123
           LK  LSK L ++YP AGR+   G+F++CND GA+++E ++   +SEF+     + +  L+
Sbjct: 65  LKKSLSKTLPIFYPLAGRII--GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELLI 124

Query: 124 RLEHDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASIARS 183
            +E     K  +    P+L +Q   F CGG ++++  SHK+ D  SLA F+  WA  +R 
Sbjct: 125 PVE----AKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 184

Query: 184 SGGGMLPEVSPLLNAASFFRPELDAGSYPSGDEGKKV----CL-KRLVFESSKIEALKAM 243
            G  ++P       A+  F   LD       D  ++V    C+ KR VF++SKI+ L+A 
Sbjct: 185 LGITLIPS----FTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 244

Query: 244 VSEK-VENPSRVQVITAFIYKAALSAKIS-VKGHWPTTSTLLQVVNIRGRMEPRLEERLI 303
            S   V+NP+RV+ +TA  ++     K+S +    P TS L  +VN+RG+++  L E  I
Sbjct: 245 ASRNLVKNPTRVEAVTALFWRCV--TKVSRLSSLTPRTSVLQILVNLRGKVD-SLCENTI 304

Query: 304 GNIVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDF---CREFPKKCRAEEWNGLYKLHA 363
           GN+++  I    K EE     +  +V +++++ E F   C+E  K       + +++L  
Sbjct: 305 GNMLSLMI---LKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSS-----SRIFEL-- 364

Query: 364 KQKMDRLISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDS-KNLIMLMDARDG 423
            +++ ++   G +  ++  +SWCK  LYDADFGWGKP+W+T       KNL++L+D +DG
Sbjct: 365 LEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDG 424

Query: 424 KGVEAIVRLEEEEMAVFQQNQELL 429
           +G+EA + L EE+M++F+ +QELL
Sbjct: 425 EGIEAWITLTEEQMSLFECDQELL 425

BLAST of Csor.00g195330 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 206.1 bits (523), Expect = 5.8e-53
Identity = 131/444 (29.50%), Postives = 229/444 (51.58%), Query Frame = 0

Query: 2   VELEIVCKQTIKPSSSTPSELRNYP----LCLLDQHAPYMSIMPLYFYDGGNTVD----S 61
           +E+ +  ++ +KPS   P  L + P    L  LDQ AP + +  L+FY     +     S
Sbjct: 3   LEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERS 62

Query: 62  GGLKDGLSKALTVYYPFAGRLQKGGNFIDCNDMGAMYLEGKLRCPMSEFMNKLQSDEVLK 121
             +K  LS+ L +YYP AGR++  G+ + CND+G  ++E K  C MS+ +     +E+ K
Sbjct: 63  DHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNK 122

Query: 122 LVRLE-HDTNGKDTDPTFNPLLSVQLFHFECGGAMVSVVFSHKVADLASLAQFVNDWASI 181
           L   E H+ +        +  L+VQL  FECGG  + +  SHK+ D  S   FVN WA+ 
Sbjct: 123 LHPFEFHEVS--------DVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAF 182

Query: 182 ARSSGGGMLPE-VSPLLNAASFFRP-ELDAGSYPSGDEGKKVCLKRLVFESSKIEALKAM 241
           AR    G   E ++P  + A  F P +++  +  +G   + +  +R VF  S +E+L+  
Sbjct: 183 AR----GQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRER 242

Query: 242 VS-EKVENPSRVQVITAFIYKAALSAKISVKGHWPTTS---TLLQVVNIRGRMEPRLEER 301
            S  K    +RV+V++ FI+   +++      H   T    TL+  VN+R + +P + + 
Sbjct: 243 FSGNKKIRATRVEVLSVFIWSRFMAS----TNHDDKTGKIYTLIHPVNLRRQADPDIPDN 302

Query: 302 LIGNIVTYFIASSTKEEEGREMEVWKIVGDMKKSFEDFCREFPKKCRAEEWNGLYKLHAK 361
           + GNI+ + +          + E   +V  M++        + KK + +    L  L+  
Sbjct: 303 MFGNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLN-- 362

Query: 362 QKMDRLISGGWDHAVYCCSSWCKFPLYDADFGWGKPIWITVPDFDSKNLIMLMDARDGKG 421
           ++    ++G  +   +  +S CKFP+Y+ADFGWGKP+W+       KNL+  +D ++G G
Sbjct: 363 KQASGFVNG--EIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDG 422

Query: 422 VEAIVRLEEEEMAVFQQNQELLSF 431
           +EA + L++ +M+ F+ ++ELL +
Sbjct: 423 IEAWINLDQNDMSRFEADEELLRY 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2K8FQU52.2e-5734.70Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
A0A2G3D6U12.9e-5734.39Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1[more]
Q58VT13.8e-5734.01Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1[more]
D2Y3X25.0e-5734.39Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1[more]
Q9ZTK58.8e-5433.48Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1... [more]
Match NameE-valueIdentityDescription
KAG6606973.10.0100.00Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subs... [more]
XP_022948443.10.099.08deacetylvindoline O-acetyltransferase-like [Cucurbita moschata][more]
XP_023524683.11.13e-30994.48vinorine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022997739.12.00e-30392.41vinorine synthase-like isoform X3 [Cucurbita maxima][more]
XP_022997736.12.03e-28286.23vinorine synthase-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G9B60.099.08deacetylvindoline O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KCE99.66e-30492.41vinorine synthase-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE... [more]
A0A6J1K8B29.81e-28386.23vinorine synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE... [more]
A0A6J1KES43.87e-26181.04vinorine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492607 PE... [more]
A0A6J1G9W86.10e-25880.59vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111452124 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G26040.19.3e-6734.68HXXXD-type acyl-transferase family protein [more]
AT3G30280.11.4e-6232.67HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.8e-6233.78HXXXD-type acyl-transferase family protein [more]
AT1G24420.12.2e-6034.01HXXXD-type acyl-transferase family protein [more]
AT1G24430.15.8e-5329.50HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..201
e-value: 1.8E-46
score: 160.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 210..431
e-value: 1.4E-53
score: 183.2
IPR003480TransferasePFAMPF02458Transferasecoord: 3..427
e-value: 1.7E-76
score: 257.7
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 3..431
NoneNo IPR availablePANTHERPTHR31623:SF51N-BENZOYLTRANSFERASE, PUTATIVE-RELATEDcoord: 3..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g195330.m01Csor.00g195330.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups