Csor.00g143290 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g143290
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionIAA-amino acid hydrolase ILR1
LocationCsor_Chr03: 8824956 .. 8827472 (+)
RNA-Seq ExpressionCsor.00g143290
SyntenyCsor.00g143290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGCGGCGGCATGGTCTTCAAGCTATTTTGTTGGATGCTGTTATTTGGGGTAGCCTTGTGGCGGCCGTCGGCGGCGGAGCACGGAGGAGATGGCTCGGAGCTCCACTCACTGACTCAGAAGCTGATGGGTTTGGCAAGGGAAGGGGATTTCATGGAGTGGATAAAAGGAGTTAGAAGGAGAATCCATGAGTATCCAGAACTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTTGACTCGCTTGGTATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTACAGGGGCTTCTCTTTCTTCTTCCATGCCTGTTTTTGCTCTAAGAGCAGACATGGATGCGCTTCCTCTTCAGGTACTCTCTCTCTCTCTCTCTCTTTTATATATATATATACACATACATATATCTATTGTCTCTTCAATCCCAAGAATGCAAAATCCCCTAAAAGAAGGAAAAAACAAGGTGATTACATGAGGAAAAAAAAAAAAAAAAAAAAAAACGCAGAATAGCATATCAAGAAACAGCTTCGACTAAACTTGTTGGCTTCCAATCCTTGCTCAATGAGTTACAATATTTCAGAAAAAAATTGAAATTCAACCTTGCTAATTGTTTTTTTCACTTGAGATGATCATACCAGGGAGTGCAAAATCTGAGTACAAGTAATGAGCAGTTTCAGATTCTCCATGCTTAATATAAACTTTATTCTCTTCAAAATTTTGAAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAAAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAGAGACAGAAACTTAAGGTAAAAATTTTCGGACTTATCCTGTTTTTTTTTTTCTTTTAACCAGGGAGGGAACTGACCAGGATTGTTTGTATATGATGTTGTTTGAAGGGAACCGTGAAGCTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAGATGCTACAAGAAGATGCCCTTGATAACATAGATGGAATCTTTGCATTGCACGTCTTGCCTTCCTTACCAACTGGTGTCATAGCCTCCAGGCCGGGTCCAGTATGTGCTGGAGCAGGACATTTTTCAGCCTTAATCCGAGGAAAGGGTGGACATGCAGCAGCCCCACATAAGACGAAGGACCCAGTTTTGGCAACAGCCTTCATAATTCAGGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCTGGGGTAAGTTTTACTCCCTCTAAATCAAGTCCCTAATTTTATCAATTTGTTAAGACCACAAGAATGAGATAACTTTTCAACAAGATTCAATACTTCAGTCCAAACAACAACTCAAAATTGTTCGAGGGCTCCCCCTCCAACAGACTCTTAAAGACTCTTAAAGGTTAGGTTACTACCTGCCTGCTTCTACGGAAGAGGTTGATTTTGTACCGACTTGAGGTCATATAGATCGGAACCCGGAGCGATAAGAATGAAAGGCCTACCCATTGCTACACCTACTGGCGCTTCAAAAGACTTAGATTCTATGCTACTGAAAGCCTTTTTTTAGGTAGGGGGGTGTAAGTCCCGAAAGCCTTTGGTACTTCGGTAGGCATATCTCAATATATCATTTTTAAAAAAACAAAAGGTTTAGAACCCTTCCCCTATTTCTGCATTTCATTCTCTGTTCGGCCCACCTCAAACAAAATGAGAAAAAAAGGAGAGAGAGACCTATTGATTCGAAGTCACTATGAAAGGGTCAGTCAATAGCATAACTTTTTCTTTCCCCGGTCTCCATTTGAAGGGAAAAGGCCTTCTCATTCCTAATCCGGTGGGGGGCCGTTCACTCATTAAACTGAAGGAAACTTGAAAATAGATCATAGTTAACTAATAATCAGCAATGTAGGTGGTAACGGTTGCATTTGTTGACGGAGGGCAAGCAGAAAATGTAGTTCCTGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCCCCTGAGGGTCTCTCTTACCTCAAGGAAAGGATCAGAGAGGTATGGCCTGTTTTTCAATTAATCAACTTAACAAATTCCCATATTTTTTAAGTTGGACAAATCTTAATTTCTACTCAACAGGTCATCAACAAACAAGCAATAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATAAAGCATTATTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGACTTCGGTTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGGGATCAAGCCGGCCGTTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCCATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTACTTGGAACACAATTCAGTTTTCAGTGATTAA

mRNA sequence

ATGGCAGGCGGCGGCATGGTCTTCAAGCTATTTTGTTGGATGCTGTTATTTGGGGTAGCCTTGTGGCGGCCGTCGGCGGCGGAGCACGGAGGAGATGGCTCGGAGCTCCACTCACTGACTCAGAAGCTGATGGGTTTGGCAAGGGAAGGGGATTTCATGGAGTGGATAAAAGGAGTTAGAAGGAGAATCCATGAGTATCCAGAACTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTTGACTCGCTTGGTATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTACAGGGGCTTCTCTTTCTTCTTCCATGCCTGTTTTTGCTCTAAGAGCAGACATGGATGCGCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAAAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAGAGACAGAAACTTAAGGGAACCGTGAAGCTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAGATGCTACAAGAAGATGCCCTTGATAACATAGATGGAATCTTTGCATTGCACGTCTTGCCTTCCTTACCAACTGGTGTCATAGCCTCCAGGCCGGGTCCAGTATGTGCTGGAGCAGGACATTTTTCAGCCTTAATCCGAGGAAAGGGTGGACATGCAGCAGCCCCACATAAGACGAAGGACCCAGTTTTGGCAACAGCCTTCATAATTCAGGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCTGGGGTGGTAACGGTTGCATTTGTTGACGGAGGGCAAGCAGAAAATGTAGTTCCTGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCCCCTGAGGGTCTCTCTTACCTCAAGGAAAGGATCAGAGAGGTCATCAACAAACAAGCAATAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATAAAGCATTATTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGACTTCGGTTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGGGATCAAGCCGGCCGTTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCCATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTACTTGGAACACAATTCAGTTTTCAGTGATTAA

Coding sequence (CDS)

ATGGCAGGCGGCGGCATGGTCTTCAAGCTATTTTGTTGGATGCTGTTATTTGGGGTAGCCTTGTGGCGGCCGTCGGCGGCGGAGCACGGAGGAGATGGCTCGGAGCTCCACTCACTGACTCAGAAGCTGATGGGTTTGGCAAGGGAAGGGGATTTCATGGAGTGGATAAAAGGAGTTAGAAGGAGAATCCATGAGTATCCAGAACTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTTGACTCGCTTGGTATCAGTTACCAGTGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTACAGGGGCTTCTCTTTCTTCTTCCATGCCTGTTTTTGCTCTAAGAGCAGACATGGATGCGCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAAAAGGTAAAATGCATGCCTGTGGTCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTGCTACAGAGTAAGAGACAGAAACTTAAGGGAACCGTGAAGCTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAGATGCTACAAGAAGATGCCCTTGATAACATAGATGGAATCTTTGCATTGCACGTCTTGCCTTCCTTACCAACTGGTGTCATAGCCTCCAGGCCGGGTCCAGTATGTGCTGGAGCAGGACATTTTTCAGCCTTAATCCGAGGAAAGGGTGGACATGCAGCAGCCCCACATAAGACGAAGGACCCAGTTTTGGCAACAGCCTTCATAATTCAGGCTCTTCAACAGATCGTATCTCGTGAGACAGATCCTCTTGAGGCTGGGGTGGTAACGGTTGCATTTGTTGACGGAGGGCAAGCAGAAAATGTAGTTCCTGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCCCCTGAGGGTCTCTCTTACCTCAAGGAAAGGATCAGAGAGGTCATCAACAAACAAGCAATAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATAAAGCATTATTTGAACATGCAAATAGAGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCTGAAGACTTCGGTTTCTTCTCACAGAGGATTGCAGCTACCATATATGGGATTGGGATAAGGAATGAGACTCTGGGATCAAGCCGGCCGTTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCCATAGGAGCAGCTCTTCATGCTGCTGTGGCCATGTCTTACTTGGAACACAATTCAGTTTTCAGTGATTAA

Protein sequence

MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNSVFSD
Homology
BLAST of Csor.00g143290 vs. ExPASy Swiss-Prot
Match: P54968 (IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2)

HSP 1 Score: 495.7 bits (1275), Expect = 5.2e-139
Identity = 243/414 (58.70%), Postives = 319/414 (77.05%), Query Frame = 0

Query: 25  SAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSEL 84
           S+A     GS L SL + ++  A++ +F EW++G+RR+IHE PE GF+E++TSQLVR EL
Sbjct: 21  SSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPETGFQEFKTSQLVRDEL 80

Query: 85  DSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMH 144
           DSLG+ Y++PVAKTGVVA I     S S PVF LRADMDALPLQELVEWE KSKV GKMH
Sbjct: 81  DSLGVKYKYPVAKTGVVAWIG----SCSKPVFGLRADMDALPLQELVEWESKSKVDGKMH 140

Query: 145 ACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQEDALDNIDGIFA 204
           ACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGEE   GAY+ML+++ LD++DGI +
Sbjct: 141 ACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKDEILDDLDGILS 200

Query: 205 LHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQI 264
           +HV PS+P+G I SRPG V AGAG F+  + G+G HAA PH +KDPVLA +  + ALQQI
Sbjct: 201 VHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLAASSAVVALQQI 260

Query: 265 VSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQA 324
           VSRE DPLEAGVVTV +++GG A+NV+P++ K GGTFRSLS +GL +++ RI+E+   QA
Sbjct: 261 VSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQRRIKEISEAQA 320

Query: 325 IVHHCYASVEFMEDT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFF 384
            V+ C A V F E      PVM N++ L+EH  +V  +++G++N    P TMG EDF FF
Sbjct: 321 SVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMIGKNNFHDFPVTMGGEDFSFF 380

Query: 385 SQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 434
           +Q+  A I+ +G++NETLG+ +PLHSP+F +DEEALP+GAALHAA+A+SYL+ +
Sbjct: 381 TQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAVSYLDEH 430

BLAST of Csor.00g143290 vs. ExPASy Swiss-Prot
Match: Q851L5 (IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL3 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.6e-132
Identity = 235/407 (57.74%), Postives = 306/407 (75.18%), Query Frame = 0

Query: 38  SLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAK 97
           +L ++L+  AR  +F  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WPVA+
Sbjct: 8   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPVAQ 67

Query: 98  TGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLG 157
           TGVVA++ GA  +   PVF LRADMDALP+QE+VEWEFKS   GKMHACGHD HVAMLLG
Sbjct: 68  TGVVATVVGA--AGPGPVFGLRADMDALPIQEMVEWEFKSLEDGKMHACGHDVHVAMLLG 127

Query: 158 AARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIA 217
           AA+LLQS+R    G VKLVFQP EE   G Y +L+E A+D++ GIF +HV   LP GV+A
Sbjct: 128 AAKLLQSRRDHFNGKVKLVFQPAEEGYAGGYYVLEEGAVDDVQGIFGMHVDAGLPAGVVA 187

Query: 218 SRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVV 277
           SRPGP  AG+  F+A I GKGGHAAAPH   DP++A +  + +LQQIV+RETDPL+  VV
Sbjct: 188 SRPGPFLAGSARFTATINGKGGHAAAPHHAVDPIVAVSSAVLSLQQIVARETDPLQGAVV 247

Query: 278 TVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFME 337
           +V  + GG+A NV+PE+V +GGT RS++ +G+SYL +RIREVI  QA V+ C A+V+FME
Sbjct: 248 SVTTIKGGEAFNVIPESVTLGGTLRSMTTDGMSYLMKRIREVIEGQAAVNRCTAAVDFME 307

Query: 338 DT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGI 397
           D     P  VN++ ++ HA  V  S+LGE+NV+L P  MGAEDFGF++QRI A  +GIG+
Sbjct: 308 DKLPPYPATVNDEEMYAHAKAVAESMLGEANVKLSPQGMGAEDFGFYAQRIPAAFFGIGV 367

Query: 398 RNETLG-----SSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
            N+  G     +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 368 GNDGGGMAETTTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 412

BLAST of Csor.00g143290 vs. ExPASy Swiss-Prot
Match: Q8H3C9 (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL7 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.1e-128
Identity = 232/417 (55.64%), Postives = 295/417 (70.74%), Query Frame = 0

Query: 24  PSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSE 83
           P+    GG G E       L+  AR   F  W++G+RR IH +PEL FEE RTS+LVR+E
Sbjct: 41  PAGGGGGGSGGE-------LLSAARAPGFAAWLRGLRRSIHRHPELAFEEVRTSELVRAE 100

Query: 84  LDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKM 143
           LD++G+ Y+WPVA+TGVVA+I G   + +  VFALRADMDALPLQELV+WE KS+  GKM
Sbjct: 101 LDAIGVPYEWPVARTGVVATIAGGDGAGAGTVFALRADMDALPLQELVDWEHKSEESGKM 160

Query: 144 HACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQEDALDNIDGIF 203
           HACGHD+H  MLLGAA+LLQS++  LKGTVKLVFQP EE   GA  +LQE  LD++  IF
Sbjct: 161 HACGHDAHTTMLLGAAKLLQSQKDDLKGTVKLVFQPAEEGYAGARYVLQEGVLDDVSAIF 220

Query: 204 ALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQ 263
            LHV P +  G + SRPGP  A +G F A I GKGGHAA PH   DP+L  +  I +LQQ
Sbjct: 221 GLHVDPRIQVGTVTSRPGPFLAASGRFLATITGKGGHAAGPHNAVDPILTASSAIVSLQQ 280

Query: 264 IVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQ 323
           IV+RETDPLEA V++V F+ GG A NV+PE+V  GGTFRSL+ EGLSYLK+RI+E++   
Sbjct: 281 IVARETDPLEAAVISVTFMKGGDAYNVIPESVSFGGTFRSLTSEGLSYLKKRIKEIVEAH 340

Query: 324 AIVHHCYASVEFMEDT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGF 383
           A VH C A+V+FME+     P  VN++ ++ HA  V   +LGE  V++    MG+EDF F
Sbjct: 341 ATVHRCTATVDFMEEERIPYPATVNDEGMYRHARAVAVDVLGEDGVKVGTPFMGSEDFAF 400

Query: 384 FSQRIAATIYGIGIRNE-TLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
           ++QR  A  + IG+ NE T+    PLHSPHFV+DE+ LP+GAALHAAVAM YL  ++
Sbjct: 401 YAQRFPAAFFMIGVGNETTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYLNKHA 450

BLAST of Csor.00g143290 vs. ExPASy Swiss-Prot
Match: Q851L6 (IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL4 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.4e-128
Identity = 230/408 (56.37%), Postives = 300/408 (73.53%), Query Frame = 0

Query: 38  SLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAK 97
           +L ++L+  AR  +F  W++G+RRRIH++PEL F+E+RTS LVR+ELD+LG++Y WP+A+
Sbjct: 4   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPIAQ 63

Query: 98  TGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLG 157
           TGVVA++ GA  +   PVFALRADMDALP+QE+VEWEFKS   GKMHACGHD+HVAMLL 
Sbjct: 64  TGVVATVAGA--AGPGPVFALRADMDALPIQEMVEWEFKSLEDGKMHACGHDAHVAMLLV 123

Query: 158 AARLLQSKRQKLKGTVKLVFQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIAS 217
           AA+LLQS+R    G VKLVFQP E   G Y +L+E  LD+   IFA+HV   LP GV+ S
Sbjct: 124 AAKLLQSRRDHFNGKVKLVFQPAEGGAGGYHVLKEGVLDDTQTIFAVHVATDLPAGVVGS 183

Query: 218 RPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVT 277
           RPGP  AG+  F+A I GKGGHAA PH   DP++A +  + +LQQIV+RET+PL+  VV+
Sbjct: 184 RPGPFLAGSARFTATITGKGGHAAEPHLAVDPIVAASSAVLSLQQIVARETNPLQGAVVS 243

Query: 278 VAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMED 337
           V  + GG+A NV+PE+V +GGT RS++ +GLSYL  RIREVI  QA V+ C A+V+FMED
Sbjct: 244 VTTIKGGEAFNVIPESVTLGGTLRSMTTDGLSYLMNRIREVIEGQAAVNRCTAAVDFMED 303

Query: 338 T----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGI- 397
                P  VN++ ++ HA  V  S+LGE+NV + P  MGAEDFGF++QRI A  +GIG+ 
Sbjct: 304 KLRPYPATVNDEGMYAHAKAVAESMLGEANVTVSPMCMGAEDFGFYAQRIPAAFFGIGVG 363

Query: 398 --RNETLG----SSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHNS 435
              N+  G    +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N+
Sbjct: 364 SNGNDGGGMAETTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKNA 409

BLAST of Csor.00g143290 vs. ExPASy Swiss-Prot
Match: Q8H3C8 (IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL8 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 5.4e-128
Identity = 226/404 (55.94%), Postives = 299/404 (74.01%), Query Frame = 0

Query: 36  LHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 95
           L +L + L+  A    F  W+ G+RRRIH+ PEL F+E RTS+LVR+ELD++G+ Y WPV
Sbjct: 37  LKALGEDLLAAAGAAGFAGWLSGLRRRIHQRPELAFQEVRTSELVRAELDAIGVPYAWPV 96

Query: 96  AKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAML 155
           A+TGVVA+I G   + + PV ALRADMDALPLQELV+WEFKS+ KGKMHACGHD+HV ML
Sbjct: 97  ARTGVVATIDGG--AGAGPVVALRADMDALPLQELVDWEFKSQEKGKMHACGHDAHVTML 156

Query: 156 LGAARLLQSKRQKLKGTVKLVFQPGEECN-GAYQMLQEDALDNIDGIFALHVLPSLPTGV 215
           LGAA+LLQS++ +LKGT+KLVFQP EE + GAY +L+   LD++  IF LHV+P+LP GV
Sbjct: 157 LGAAKLLQSRKDELKGTIKLVFQPAEEGHAGAYHVLESGLLDDVSVIFGLHVIPNLPVGV 216

Query: 216 IASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAG 275
           +ASRPGP  + A  F+A   GKGGHA  PH   DPV+A +  + +LQQ+VSRETDPLEA 
Sbjct: 217 VASRPGPFMSAAARFAATFTGKGGHAGVPHDAVDPVVAVSSAVLSLQQLVSRETDPLEAA 276

Query: 276 VVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEF 335
           VV++  + GG A NV+PE+  +GGTFRS++ EGL+YL +RIRE+I  QA V+ C A+V+F
Sbjct: 277 VVSITILKGGDAYNVIPESASLGGTFRSMTDEGLAYLMKRIREIIEAQAGVNRCAAAVDF 336

Query: 336 MEDT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGI 395
           +E+     P  VN+  ++ HA  V  ++LGE+NV++   +MG EDF F+++R     + I
Sbjct: 337 LEEELRPYPATVNDDGMYGHAKAVAEAMLGEANVRVAARSMGGEDFAFYARRSPGAFFFI 396

Query: 396 GIRNET----LGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           G+ NET      + RP+HSPHFVLDE ALP+GAALHAAVA+ YL
Sbjct: 397 GVGNETTMGPAAAVRPVHSPHFVLDERALPVGAALHAAVAIEYL 438

BLAST of Csor.00g143290 vs. NCBI nr
Match: XP_022925994.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034481.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0

Query: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60
           MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR
Sbjct: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300
           AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360

Query: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420
           ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 438
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Csor.00g143290 vs. NCBI nr
Match: XP_023543889.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862 bits (2227), Expect = 1.75e-315
Identity = 430/436 (98.62%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 3   GGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRR 62
           GGGM FKLFCWML FGVALWRPSAA HGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRR
Sbjct: 4   GGGMDFKLFCWMLFFGVALWRPSAAGHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRR 63

Query: 63  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADM 122
           IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSS PVFALRADM
Sbjct: 64  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRADM 123

Query: 123 DALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE 182
           DALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE
Sbjct: 124 DALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE 183

Query: 183 CNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAA 242
           CNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAA
Sbjct: 184 CNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAA 243

Query: 243 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRS 302
           PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRS
Sbjct: 244 PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRS 303

Query: 303 LSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLGES 362
           LSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNN+ALFEHANRVGNSLLGES
Sbjct: 304 LSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLGES 363

Query: 363 NVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALH 422
           NVQLLPWTMGAEDFGFFSQRIAATIYGIG+RNETLGSSRPLHSPHFVLDEEALPIGAALH
Sbjct: 364 NVQLLPWTMGAEDFGFFSQRIAATIYGIGVRNETLGSSRPLHSPHFVLDEEALPIGAALH 423

Query: 423 AAVAMSYLEHNSVFSD 438
           AAVAMSYLEHNSVFSD
Sbjct: 424 AAVAMSYLEHNSVFSD 439

BLAST of Csor.00g143290 vs. NCBI nr
Match: XP_022979169.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima])

HSP 1 Score: 856 bits (2211), Expect = 6.31e-313
Identity = 429/438 (97.95%), Postives = 433/438 (98.86%), Query Frame = 0

Query: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60
           MAGGGM FKLFC ML FGVALWRPSAAEHGGDGS+LHSLTQKLMGLAREG+FMEWIKGVR
Sbjct: 1   MAGGGMDFKLFCSMLFFGVALWRPSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300
           AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNN+ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLG 360

Query: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420
           ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 438
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Csor.00g143290 vs. NCBI nr
Match: XP_038882070.1 (IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida])

HSP 1 Score: 808 bits (2088), Expect = 8.04e-294
Identity = 404/439 (92.03%), Postives = 419/439 (95.44%), Query Frame = 0

Query: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60
           MAGGGM FKLFC ML+FG+ LWRPS AEHGGDG ELHSLTQKL+GLAREG+FMEWIKGVR
Sbjct: 15  MAGGGMAFKLFCCMLVFGMVLWRPSTAEHGGDGLELHSLTQKLLGLAREGEFMEWIKGVR 74

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSM-PVFALR 120
           RRIH+YPELGFEEY+TSQLVRSELDSLGISYQWPVAKTGVVASI G S+SSS  PVF LR
Sbjct: 75  RRIHQYPELGFEEYKTSQLVRSELDSLGISYQWPVAKTGVVASIRGDSVSSSSAPVFGLR 134

Query: 121 ADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQP 180
           ADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSKR KLKGTVKLVFQP
Sbjct: 135 ADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQP 194

Query: 181 GEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGH 240
           GEECNGAYQMLQEDALD+IDGIFALHV PSLPTGVIASRPGPVCAGAGHFSALI+GKGGH
Sbjct: 195 GEECNGAYQMLQEDALDDIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIQGKGGH 254

Query: 241 AAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGT 300
           AAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVP TVK+GGT
Sbjct: 255 AAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPGTVKLGGT 314

Query: 301 FRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLL 360
           FRSLSPEG SYLKERIREVIN QAIVHHCYASVEFMEDTPVMVNN+ALFEHA RVGNSLL
Sbjct: 315 FRSLSPEGFSYLKERIREVINTQAIVHHCYASVEFMEDTPVMVNNEALFEHAKRVGNSLL 374

Query: 361 GESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGA 420
           GE NVQLLPWTMGAEDFGFFSQRIAATIY IG  NETLGS+RP+HSPHFVLDEEALPIGA
Sbjct: 375 GEPNVQLLPWTMGAEDFGFFSQRIAATIYVIGTGNETLGSNRPVHSPHFVLDEEALPIGA 434

Query: 421 ALHAAVAMSYLEHNSVFSD 438
           ALHAAVAM+YLEHNSVFSD
Sbjct: 435 ALHAAVAMTYLEHNSVFSD 453

BLAST of Csor.00g143290 vs. NCBI nr
Match: XP_022962856.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata])

HSP 1 Score: 803 bits (2074), Expect = 1.18e-291
Identity = 402/437 (91.99%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 3   GGGMVFKLFCWMLLFGVALWRPSAAE--HGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 62
           GGGM FKLFCWML+FGVALWRPSA+E  HGGDG ELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 26  GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 85

Query: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSM----PVF 122
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASI GAS SSS     PVF
Sbjct: 86  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 145

Query: 123 ALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLV 182
           ALRADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 146 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 205

Query: 183 FQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242
           FQPGEECNGAYQ+LQEDALD+IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 206 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 265

Query: 243 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 302
           GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 266 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 325

Query: 303 GGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGN 362
           GGTFRSLSP+GLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGN
Sbjct: 326 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 385

Query: 363 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALP 422
           SLLGESNVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TL S+RPLHSPHFVLDEEALP
Sbjct: 386 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 445

Query: 423 IGAALHAAVAMSYLEHN 433
           IGAALHAAVAMSYLEHN
Sbjct: 446 IGAALHAAVAMSYLEHN 462

BLAST of Csor.00g143290 vs. ExPASy TrEMBL
Match: A0A6J1EDS0 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111433244 PE=3 SV=1)

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0

Query: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60
           MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR
Sbjct: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300
           AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360

Query: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420
           ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 438
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Csor.00g143290 vs. ExPASy TrEMBL
Match: A0A6J1IVY2 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889 PE=3 SV=1)

HSP 1 Score: 856 bits (2211), Expect = 3.06e-313
Identity = 429/438 (97.95%), Postives = 433/438 (98.86%), Query Frame = 0

Query: 1   MAGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 60
           MAGGGM FKLFC ML FGVALWRPSAAEHGGDGS+LHSLTQKLMGLAREG+FMEWIKGVR
Sbjct: 1   MAGGGMDFKLFCSMLFFGVALWRPSAAEHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVR 60

Query: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRA 120
           RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSS PVFALRA
Sbjct: 61  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSTPVFALRA 120

Query: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPG 180
           DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLL+SKR KLKGTVKLVFQPG
Sbjct: 121 DMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLVFQPG 180

Query: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240
           EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA
Sbjct: 181 EECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHA 240

Query: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300
           AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF
Sbjct: 241 AAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTF 300

Query: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLG 360
           RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNN+ALFEHANRVGNSLLG
Sbjct: 301 RSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGNSLLG 360

Query: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420
           ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA
Sbjct: 361 ESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAA 420

Query: 421 LHAAVAMSYLEHNSVFSD 438
           LHAAVAMSYLEHNSVFSD
Sbjct: 421 LHAAVAMSYLEHNSVFSD 438

BLAST of Csor.00g143290 vs. ExPASy TrEMBL
Match: A0A6J1HEG5 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111463223 PE=3 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 5.70e-292
Identity = 402/437 (91.99%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 3   GGGMVFKLFCWMLLFGVALWRPSAAE--HGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 62
           GGGM FKLFCWML+FGVALWRPSA+E  HGGDG ELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 26  GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 85

Query: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSM----PVF 122
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASI GAS SSS     PVF
Sbjct: 86  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 145

Query: 123 ALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLV 182
           ALRADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 146 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 205

Query: 183 FQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242
           FQPGEECNGAYQ+LQEDALD+IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 206 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 265

Query: 243 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 302
           GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 266 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 325

Query: 303 GGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGN 362
           GGTFRSLSP+GLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGN
Sbjct: 326 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 385

Query: 363 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALP 422
           SLLGESNVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TL S+RPLHSPHFVLDEEALP
Sbjct: 386 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 445

Query: 423 IGAALHAAVAMSYLEHN 433
           IGAALHAAVAMSYLEHN
Sbjct: 446 IGAALHAAVAMSYLEHN 462

BLAST of Csor.00g143290 vs. ExPASy TrEMBL
Match: A0A0A0KIR8 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 PE=3 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 5.32e-285
Identity = 390/438 (89.04%), Postives = 412/438 (94.06%), Query Frame = 0

Query: 2   AGGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRR 61
           AGGG  FK  CWM++FG+ALWRP+A E+GGDGSELHSLTQ L+GLARE +FMEWIKGVRR
Sbjct: 3   AGGGTSFKSLCWMIVFGIALWRPAAGENGGDGSELHSLTQSLLGLAREREFMEWIKGVRR 62

Query: 62  RIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSM--PVFALR 121
           RIHEYPELGFEEY+TSQLVRSELDSLGISY+WPVAKTGVVASI G S+SSS   PVF LR
Sbjct: 63  RIHEYPELGFEEYKTSQLVRSELDSLGISYRWPVAKTGVVASIRGDSVSSSSSTPVFGLR 122

Query: 122 ADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQP 181
           ADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAM+LGAARLLQS R+KLKGTVKLVFQP
Sbjct: 123 ADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMVLGAARLLQSIREKLKGTVKLVFQP 182

Query: 182 GEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGH 241
            EECNGAYQML++DALD IDGIFALHV PSLPTGVIASRPGPVCAGAGHFSALIRGKGGH
Sbjct: 183 AEECNGAYQMLKDDALDGIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIRGKGGH 242

Query: 242 AAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGT 301
           AA PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGT
Sbjct: 243 AATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGT 302

Query: 302 FRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLL 361
           FRSLSPEG SYLKERIREVI+ QA+VHHCYASV+FMEDTPVMVNN+ALFEH NRVGNSLL
Sbjct: 303 FRSLSPEGFSYLKERIREVISTQAMVHHCYASVKFMEDTPVMVNNEALFEHVNRVGNSLL 362

Query: 362 GESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGA 421
           GESNVQLLPWTMGAEDFGFFSQRI ATIY IG  NETLGS+RP+HSPHFVLDEEALPIGA
Sbjct: 363 GESNVQLLPWTMGAEDFGFFSQRIPATIYVIGTGNETLGSNRPVHSPHFVLDEEALPIGA 422

Query: 422 ALHAAVAMSYLEHNSVFS 437
           ALHAAVA +YLEHNSVFS
Sbjct: 423 ALHAAVATTYLEHNSVFS 440

BLAST of Csor.00g143290 vs. ExPASy TrEMBL
Match: A0A5A7SGG7 (IAA-amino acid hydrolase ILR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001650 PE=3 SV=1)

HSP 1 Score: 784 bits (2025), Expect = 7.27e-285
Identity = 390/437 (89.24%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 3   GGGMVFKLFCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRR 62
           GGG  FK  CWM++FG+ALWRP+A EHGGDGSEL SLTQ  + LARE +FMEWIKGVRRR
Sbjct: 4   GGGTSFKSLCWMMVFGMALWRPAAGEHGGDGSELDSLTQSFLDLAREREFMEWIKGVRRR 63

Query: 63  IHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSM-PVFALRAD 122
           IHEYPELGFEEY+TSQLVRSELDSLGISY+WPVAKTGVVASITG S+SSS  PVF LRAD
Sbjct: 64  IHEYPELGFEEYKTSQLVRSELDSLGISYRWPVAKTGVVASITGDSVSSSSTPVFGLRAD 123

Query: 123 MDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGE 182
           MDALPLQELVEW+FKSKV+GKMHACGHDSHVAM+LGAARLLQS R+KLKGTVKLVFQPGE
Sbjct: 124 MDALPLQELVEWKFKSKVEGKMHACGHDSHVAMVLGAARLLQSIREKLKGTVKLVFQPGE 183

Query: 183 ECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA 242
           ECNGAYQML++DALD IDGIFALHV PSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA
Sbjct: 184 ECNGAYQMLKDDALDGIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAA 243

Query: 243 APHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFR 302
            PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFR
Sbjct: 244 TPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFR 303

Query: 303 SLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGNSLLGE 362
           SLSPEGLSYLKERIREVIN QAIVHHCYASVEFMEDTPVMVNN+ALFEH NRVGNSLLGE
Sbjct: 304 SLSPEGLSYLKERIREVINTQAIVHHCYASVEFMEDTPVMVNNEALFEHVNRVGNSLLGE 363

Query: 363 SNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAAL 422
           SNVQLLPWTMGAEDFGFFSQRI ATIY IG  NETLGS+RP+HSPHF LDEEALPIGAAL
Sbjct: 364 SNVQLLPWTMGAEDFGFFSQRIPATIYVIGTGNETLGSNRPVHSPHFSLDEEALPIGAAL 423

Query: 423 HAAVAMSYLEHNSVFSD 438
           HAAVA +YLEHNSVF++
Sbjct: 424 HAAVATTYLEHNSVFTN 440

BLAST of Csor.00g143290 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 495.7 bits (1275), Expect = 3.7e-140
Identity = 243/414 (58.70%), Postives = 319/414 (77.05%), Query Frame = 0

Query: 25  SAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSEL 84
           S+A     GS L SL + ++  A++ +F EW++G+RR+IHE PE GF+E++TSQLVR EL
Sbjct: 21  SSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPETGFQEFKTSQLVRDEL 80

Query: 85  DSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMH 144
           DSLG+ Y++PVAKTGVVA I     S S PVF LRADMDALPLQELVEWE KSKV GKMH
Sbjct: 81  DSLGVKYKYPVAKTGVVAWIG----SCSKPVFGLRADMDALPLQELVEWESKSKVDGKMH 140

Query: 145 ACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQEDALDNIDGIFA 204
           ACGHD+HVAMLLGAA+LLQ+ +  +KGTVKLVFQPGEE   GAY+ML+++ LD++DGI +
Sbjct: 141 ACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKDEILDDLDGILS 200

Query: 205 LHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQI 264
           +HV PS+P+G I SRPG V AGAG F+  + G+G HAA PH +KDPVLA +  + ALQQI
Sbjct: 201 VHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLAASSAVVALQQI 260

Query: 265 VSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQA 324
           VSRE DPLEAGVVTV +++GG A+NV+P++ K GGTFRSLS +GL +++ RI+E+   QA
Sbjct: 261 VSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQRRIKEISEAQA 320

Query: 325 IVHHCYASVEFMEDT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFF 384
            V+ C A V F E      PVM N++ L+EH  +V  +++G++N    P TMG EDF FF
Sbjct: 321 SVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMIGKNNFHDFPVTMGGEDFSFF 380

Query: 385 SQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 434
           +Q+  A I+ +G++NETLG+ +PLHSP+F +DEEALP+GAALHAA+A+SYL+ +
Sbjct: 381 TQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAVSYLDEH 430

BLAST of Csor.00g143290 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 385.2 bits (988), Expect = 7.1e-107
Identity = 198/400 (49.50%), Postives = 270/400 (67.50%), Query Frame = 0

Query: 36  LHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPV 95
           L  +  K + LA+  DF +W+ G+RRRIHE PELG+EE  TS+LVR+EL+ +G+SY++PV
Sbjct: 28  LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPV 87

Query: 96  AKTGVVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAML 155
           A TGVV  +     +   P  ALRADMDAL +QE+VEWE KSKV GKMHACGHD+H  ML
Sbjct: 88  AVTGVVGYVG----TGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTML 147

Query: 156 LGAARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQEDALDNIDGIFALHVLPSLPTGV 215
           LGAA+LL+   ++L+GTV LVFQP EE   GA ++++   L+N+  IF LHV   L  G 
Sbjct: 148 LGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALGQ 207

Query: 216 IASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAG 275
           ++SR GP+ AG+G F A I GKGGHAA P  T DP+LA + +I +LQ +VSRE DPL++ 
Sbjct: 208 VSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDSQ 267

Query: 276 VVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEF 335
           VVTVA  +GG A NV+P++V +GGTFR+ S +    LK+RI +VI +QA V+ C A+V+F
Sbjct: 268 VVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVDF 327

Query: 336 MEDT----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGI 395
           +E+     P  VN+KAL +    V   +LG  N   +   MG+EDF F+ Q I      +
Sbjct: 328 IEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFV 387

Query: 396 GIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           G++N+        HSP+F ++EE LP GA+LHA++A  YL
Sbjct: 388 GMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYL 423

BLAST of Csor.00g143290 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 380.2 bits (975), Expect = 2.3e-105
Identity = 205/396 (51.77%), Postives = 266/396 (67.17%), Query Frame = 0

Query: 40  TQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTG 99
           + +++ L  + D + W+K VRR IHE PEL FEEY TS+L+RSELD +GI Y++P+AKTG
Sbjct: 73  SDEILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTG 132

Query: 100 VVASITGASLSSSMPVFALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAA 159
           + A I     S   P  A+RADMDALP+QE VEWE  SKV GKMHACGHD+HV MLLGAA
Sbjct: 133 IRAWIG----SGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAA 192

Query: 160 RLLQSKRQKLKGTVKLVFQPGEEC-NGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASR 219
            +L+++   LKGTV L+FQP EE  NGA  M+++ ALD+++ IFA+HV    PTGVI SR
Sbjct: 193 HILKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSR 252

Query: 220 PGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTV 279
            GP+ AG G F A+I  +    AA     + +LA +  + +LQ IVSRE  PL++ VV+V
Sbjct: 253 SGPLLAGCGIFRAVITSEDSRGAA-----NLLLAASSAVISLQGIVSREASPLDSQVVSV 312

Query: 280 AFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDT 339
              DGG + +V P+TV +GGTFR+ S     YLK+RI+EV+  Q  V  C A+V F E  
Sbjct: 313 TSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQ 372

Query: 340 ----PVMVNNKALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRN 399
               P   NN A + H  +V   LLG+S+  L P  MGAEDF F+S+ I A  Y IGIRN
Sbjct: 373 NAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRN 432

Query: 400 ETLGSSRPLHSPHFVLDEEALPIGAALHAAVAMSYL 431
           E LGS    HSPHF++DE++LP+GAA+HAAVA  YL
Sbjct: 433 EELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of Csor.00g143290 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-103
Identity = 205/427 (48.01%), Postives = 286/427 (66.98%), Query Frame = 0

Query: 14  MLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELGFEE 73
           + L  V+   P  AE   D S++ +   KL+  A+  +  +W+  +RR+IHE PELG+EE
Sbjct: 15  LFLLSVSSESPWIAE---DTSQIQT---KLLEFAKSPEVFDWMVKIRRKIHENPELGYEE 74

Query: 74  YRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQELVEW 133
             TS+L+RSEL+ +GI Y++PVA TGV+  I     +   P  ALRADMDALP+QE VEW
Sbjct: 75  LETSKLIRSELELIGIKYRYPVAITGVIGYIG----TGEPPFVALRADMDALPIQEGVEW 134

Query: 134 EFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEE-CNGAYQMLQE 193
           E KSK+ GKMHACGHD HV MLLGAA++L   R  L+GTV L+FQP EE  +GA +M +E
Sbjct: 135 EHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREE 194

Query: 194 DALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLA 253
            AL N++ IF +H+   +P G  ASR G   AGAG F A+I GKGGHAA P  T DPV+A
Sbjct: 195 GALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVA 254

Query: 254 TAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLSYLK 313
            + I+ +LQQ+VSRETDPL++ VVTV+ V+GG A NV+P+++ +GGT R+ +  G + L+
Sbjct: 255 ASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQ 314

Query: 314 ERIREVINKQAIVHHCYASVEF----MEDTPVMVNNKALFEHANRVGNSLLG-ESNVQLL 373
           +R++EVI KQA VH C ASV       E  P  VNNK L++   +V   LLG E+ V+  
Sbjct: 315 QRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAA 374

Query: 374 PWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAAVAM 433
           P  MG+EDF +F++ I      +G+++ET G +   HSP + ++E+ LP GAA+HA++A+
Sbjct: 375 P-VMGSEDFSYFAETIPGHFSLLGMQDETNGYASS-HSPLYRINEDVLPYGAAIHASMAV 427

Query: 434 SYLEHNS 435
            YL+  +
Sbjct: 435 QYLKEKA 427

BLAST of Csor.00g143290 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-102
Identity = 202/430 (46.98%), Postives = 281/430 (65.35%), Query Frame = 0

Query: 11  FCWMLLFGVALWRPSAAEHGGDGSELHSLTQKLMGLAREGDFMEWIKGVRRRIHEYPELG 70
           FC ++ F + L   ++       ++L  + +  + LA+  DF +W+ G+RRRIHE PELG
Sbjct: 3   FCKLVSFVLILHLLNSCLISCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELG 62

Query: 71  FEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSSMPVFALRADMDALPLQEL 130
           +EE  TS+LV++ELD +G+SY+ PVA TGV+  +     +   P  ALRADMDALP+QE+
Sbjct: 63  YEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVG----TGHAPFVALRADMDALPIQEM 122

Query: 131 VEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLVFQPGEECN-GAYQM 190
           VEWE KSK+ GKMHACGHD+H  MLLGAA+LL+  +++L+GTV LVFQP EE   GA ++
Sbjct: 123 VEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKI 182

Query: 191 LQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDP 250
           ++   L+N+  IF LHV   L  G ++SR G + AG+G F A I GKGGHAA P    DP
Sbjct: 183 VEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDP 242

Query: 251 VLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKVGGTFRSLSPEGLS 310
           VLA + +I +LQ +VSRE DPL++ VVTVA  +G  A NV+P++V +GGTFR+L P+   
Sbjct: 243 VLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFE 302

Query: 311 YLKERIREVINKQAIVHHCYASVEFMEDT----PVMVNNKALFEHANRVGNSLLG-ESNV 370
            LK+RI +VI  QA V+ C A+V+F+ED     P  VNNK L      V   +LG E+ V
Sbjct: 303 QLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYV 362

Query: 371 QLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALPIGAALHAA 430
           + LP  M +EDF F+ Q I      +G++N++       HSP F ++EE LP GA+L A+
Sbjct: 363 ETLP-VMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLAS 422

Query: 431 VAMSYLEHNS 435
           +A  YL  +S
Sbjct: 423 LATRYLLDSS 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P549685.2e-13958.70IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2[more]
Q851L51.6e-13257.74IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C91.1e-12855.64IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q851L62.4e-12856.37IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C85.4e-12855.94IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
XP_022925994.10.0100.00IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amin... [more]
XP_023543889.11.75e-31598.62IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo][more]
XP_022979169.16.31e-31397.95IAA-amino acid hydrolase ILR1-like 3 [Cucurbita maxima][more]
XP_038882070.18.04e-29492.03IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida][more]
XP_022962856.11.18e-29191.99IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1EDS00.0100.00IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114332... [more]
A0A6J1IVY23.06e-31397.95IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889... [more]
A0A6J1HEG55.70e-29291.99IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114632... [more]
A0A0A0KIR85.32e-28589.04M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 P... [more]
A0A5A7SGG77.27e-28589.24IAA-amino acid hydrolase ILR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT3G02875.13.7e-14058.70Peptidase M20/M25/M40 family protein [more]
AT1G51760.17.1e-10749.50peptidase M20/M25/M40 family protein [more]
AT1G44350.12.3e-10551.77IAA-leucine resistant (ILR)-like gene 6 [more]
AT5G56660.11.2e-10348.01IAA-leucine resistant (ILR)-like 2 [more]
AT1G51780.11.1e-10246.98IAA-leucine resistant (ILR)-like gene 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 233..323
e-value: 9.3E-11
score: 41.6
NoneNo IPR availableGENE3D3.30.70.360coord: 224..338
e-value: 3.6E-132
score: 442.5
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 59..415
e-value: 3.6E-132
score: 442.5
NoneNo IPR availablePANTHERPTHR11014:SF108IAA-AMINO ACID HYDROLASE ILR1coord: 7..433
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 51..432
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 58..418
e-value: 1.9E-113
score: 377.2
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 45..434
e-value: 6.8E-118
score: 391.9
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 7..433
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 117..426
e-value: 2.6E-32
score: 112.7
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 56..430
e-value: 0.0
score: 593.527
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 227..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g143290.m01Csor.00g143290.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity