Csor.00g036890 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g036890
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr09: 5322503 .. 5325133 (+)
RNA-Seq ExpressionCsor.00g036890
SyntenyCsor.00g036890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCGCCCTCCCTCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCTTCGGTCTCTCCGATCACGCCTCCGCCCGTGCCGCTGCTGCTCCTGCTGCTGCTGTTGTTGCTGCTGGAGGTGGTGATAGATATGCTATCAGTTGGGATGACTTGCGTGTGGATGTTCCGTCGCGCCAGGGGCTCAGCTCCGGTGTGCATGGCGGCGGGGTTCAGAATGGAACTCGAACTAGAATCATTGTGGTGGATAAAACCTCCTCCGGCGACTCCACCACCGTGCAAGGGGCGGTGGATTTGGTTCCTCATAATAATAATCACAGAGTTAAGATTTATATTCGTCCTGGCATTTACAGGTAACTTCTTTTATTGTATTATTTCATCCTTCCTAGGAATGTTTTTAAAACGTTCGTCGTGTCGTGGTCGTGATCTTGATCTTGATCTTATTCGTAGTTAGGAATGGGGAAGAGGTCTAAACTAATGGACATTAATAAACCGTCATTCATGGACGTTACAAATGACACACAATCAATTTGTTTCAAGATCGTAGGCAGCACCGTACGTTGTATCGATCATTAGATCAATTCATTGAAAGCTTTCAAAATTAGATTCTATCTCCGAGATTTTGGCCTTAAATTCAAAATATTTTCTTAAGAAAGATGAAAATTACCGTAGAAAAATCCGAGGTTTGTATCGAGTTAGGCTCTTATTTTTTTAAAAAAATATCTAATATATTTCAAAATATTTTTTTTAAAAATATCTAATATATTCCAAAATTTCAATCATATGTTTAATAAATCTCGATTTTTAAATAATTAATTGACCTACTACGTATAAAAAAATAATAATTTTTACATCTAATAAACCCTAAACTTTAAAACAAATTTATATTTAGCAAAATCGAGAAATTTAAAAAATACCAAAATTAAAGTTACTATAAACATATTAGATATCACCCATAAAAAATTAAAGCTCTATGAAATTAATCGGGTTTTATTTTCTTCGGAATGTATATATACATGTATGTATGTGTATATATGTGTGTGTATGTATGTACATACATATATATATATAATAAATCTCTAAATTTTTGTAAAGAAATATATGTTGTTTTGAATTGATCTACAAAACCAAGATTTGATGATGAACTAAAGTGTATTGTAATATGAACTTGCAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCATTGATAGGAAACAGGAGACGAATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGGTTAGCAGCATAAACAACAAAGATAAACAAAGATAAGAGTATTAAGTATGAAGTTACTGAGTTTTGTAAGTTCAATGTGGTTTGGTTTGTAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTGTGGCTCTAAGGATTTCAGGAGAGAAGGCTATGTTTTATAGAGTCAAGTTCTTGGGACAACAAGATACACTTTTGGATGATATGGGCACACACTATTTCTATCAATGTCACATTCAAGGGACTATTGATTTCATATTTGGTACGGCTAGATCGCTCTATGAGGTAACGAGTCTCGTTACTAGACATGTAGTTGATACCTTTCGAGTTTTGAACTTGTCGTCTGATGTTTGTCTGTTTTTTTTCTCTCTTGAAAACCAGCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCACCGCACGATGACACCGGGTTCTCTTTCGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGTAAGGCAAATATACTTACAATAACGACCCCCCGACACATACACACACATACCATGACATTAACCGAGGTGGGCGTAAGTTGACCCGAACACTCATAAGAATCATAAATATCCCTCCACCTCTTGAATCAAGCTAATCTAATAGAAATTTTCTATTGTTATCAAGACAAAGTATACTTACAATAACGACCCCCCCGACACACACATCCCATGAGATTAACCGAGGTGGGTGTAGGTTGACCCGAACACTTACAAGAATTATAAATATCCCTCCACCTCTTGAATCAAGCTAATATTGTTATAAGACAAAGGTAAAAGGAAAAGTACACTTACAATAACGACCCCCCGACCCCCGACACATACACACACATCCTGTGAGATTAACCGAGGTGGGCATAGGTTGACCCGAACACTCACAAGAATTATAAATATCCCTCCACCTCATGAATCAAGCTAACCTAATAGAACTTTTCAATTGCTATCCACACACAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGA

mRNA sequence

ATGACCGCCCTCCCTCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCTTCGGTCTCTCCGATCACGCCTCCGCCCGTGCCGCTGCTGCTCCTGCTGCTGCTGTTGTTGCTGCTGGAGGTGGTGATAGATATGCTATCAGTTGGGATGACTTGCGTGTGGATGTTCCGTCGCGCCAGGGGCTCAGCTCCGGTGTGCATGGCGGCGGGGTTCAGAATGGAACTCGAACTAGAATCATTGTGGTGGATAAAACCTCCTCCGGCGACTCCACCACCGTGCAAGGGGCGGTGGATTTGGTTCCTCATAATAATAATCACAGAGTTAAGATTTATATTCGTCCTGGCATTTACAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCATTGATAGGAAACAGGAGACGAATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTGTGGCTCTAAGGATTTCAGGAGAGAAGGCTATGTTTTATAGAGTCAAGTTCTTGGGACAACAAGATACACTTTTGGATGATATGGGCACACACTATTTCTATCAATGTCACATTCAAGGGACTATTGATTTCATATTTGGTACGGCTAGATCGCTCTATGAGCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCACCGCACGATGACACCGGGTTCTCTTTCGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGA

Coding sequence (CDS)

ATGACCGCCCTCCCTCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCTTCGGTCTCTCCGATCACGCCTCCGCCCGTGCCGCTGCTGCTCCTGCTGCTGCTGTTGTTGCTGCTGGAGGTGGTGATAGATATGCTATCAGTTGGGATGACTTGCGTGTGGATGTTCCGTCGCGCCAGGGGCTCAGCTCCGGTGTGCATGGCGGCGGGGTTCAGAATGGAACTCGAACTAGAATCATTGTGGTGGATAAAACCTCCTCCGGCGACTCCACCACCGTGCAAGGGGCGGTGGATTTGGTTCCTCATAATAATAATCACAGAGTTAAGATTTATATTCGTCCTGGCATTTACAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCATTGATAGGAAACAGGAGACGAATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTGTGGCTCTAAGGATTTCAGGAGAGAAGGCTATGTTTTATAGAGTCAAGTTCTTGGGACAACAAGATACACTTTTGGATGATATGGGCACACACTATTTCTATCAATGTCACATTCAAGGGACTATTGATTTCATATTTGGTACGGCTAGATCGCTCTATGAGCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCACCGCACGATGACACCGGGTTCTCTTTCGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGA

Protein sequence

MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL
Homology
BLAST of Csor.00g036890 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.2e-117
Identity = 204/342 (59.65%), Postives = 253/342 (73.98%), Query Frame = 0

Query: 45  ISWDDLRVDVPSRQGLSSGV---------HGGGVQNGTRT-RIIVVDKTSSGDSTTVQGA 104
           ISW+DLRV    R   S  +         +     N T   R+IVVDK   GDS TVQGA
Sbjct: 39  ISWEDLRVVEDGRIERSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGA 98

Query: 105 VDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVS 164
           VD+VP +N+ RVKI+I PGIYREKV +P +KPYIS IGN     DTVI+ + KASD    
Sbjct: 99  VDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCD 158

Query: 165 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 224
           G ELGTYRTA+V+I+SD+FCA+ ITFENTVV+  G++GRQAVALRI G+KA+FYRV+ LG
Sbjct: 159 GKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLG 218

Query: 225 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDD 284
            QDTL DD G+HYFYQC+IQG +DFIFG A+SLY+ C I STA+ +GAIAAHHR S  +D
Sbjct: 219 SQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETED 278

Query: 285 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 344
           TGFSFV C I+G+G++ LGRAWG YSRT+YS C+I D+I+P+GWSDW  P RQR  +FG+
Sbjct: 279 TGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGE 338

Query: 345 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 377
           Y CRG GA   G VPW++T T +EV+P++ R FI G+QWL L
Sbjct: 339 YNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

BLAST of Csor.00g036890 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 7.1e-76
Identity = 136/301 (45.18%), Postives = 194/301 (64.45%), Query Frame = 0

Query: 77  IIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRR 136
           + V  K++ GD T +Q A+D +P  N  RV I +  G+Y+EKV IP  K +I++ G    
Sbjct: 85  LTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAE 144

Query: 137 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA 196
              T +     A   D  G  +GTY +A+ A++S +F A  ITF NT  V  PG  G+QA
Sbjct: 145 --KTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQA 204

Query: 197 VALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITS 256
           VALR+S + A F+  + LG QDTL D +G HY+  C+I+G++DFIFG A SLYE C + +
Sbjct: 205 VALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHA 264

Query: 257 TAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISP 316
            A+  GA+ A  RSS  +DTGFSFV+C + G+G + LGRAWG +SR +++Y Y++++I P
Sbjct: 265 IADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILP 324

Query: 317 LGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLN 376
            GW +W DPSR+ T  +GQY+C G+GAN  G V WAR  T EE +P++   FI+G +W+ 
Sbjct: 325 RGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 383

BLAST of Csor.00g036890 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 5.1e-74
Identity = 138/301 (45.85%), Postives = 187/301 (62.13%), Query Frame = 0

Query: 76  RIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRR 135
           ++I V         +VQ AVD +P NNN  + I I PG YREKV +P TKPYI+  G  R
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 136 RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQ 195
            +  T I  + +ASD   +G +L TY+TA+V + ++YF A  I+F NT     PG +G Q
Sbjct: 119 DV--TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQ 178

Query: 196 AVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVIT 255
           AVA RISG+KA F    F G QDTL DD G HYF +C+I+G+IDFIFG  RS+Y+ C + 
Sbjct: 179 AVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELH 238

Query: 256 STAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMIS 315
           S A  FG+IAAH R+ P + TGF+FV C + G+G + +GRA G YSR +Y+Y Y + +++
Sbjct: 239 SIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVA 298

Query: 316 PLGWSDWSDPS-RQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQW 375
             GW DW   S + +TA FG Y C G GA     V WAR    E   P++ ++F+NG  W
Sbjct: 299 HGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHW 357

BLAST of Csor.00g036890 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 5.8e-62
Identity = 132/309 (42.72%), Postives = 180/309 (58.25%), Query Frame = 0

Query: 75  TRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNR 134
           T  + VDK    + TTVQ AVD V + +  R  I+I  G+Y EKV IP TKP I+L G  
Sbjct: 88  TSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQG-- 147

Query: 135 RRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGR 194
           +    T I  N  A   +      GT+  ATV +    F A  I+F N   +  PGD G 
Sbjct: 148 QGFDITAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGA 207

Query: 195 QAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVI 254
           QAVA+RI+G+++ F    F G QDTL DD G HYF  C+IQG+IDFIFG A+SLY+ C I
Sbjct: 208 QAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRI 267

Query: 255 TSTAETF--------GAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYS 314
            S A           GA+ A+ RSS  +++GFSFV C I G+G V LGRAW  YSR ++ 
Sbjct: 268 ISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFV 327

Query: 315 YCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDR 374
              + D+I+P GW++++DPSR  T  +G+Y C G GA+     P+ +     +V   ++ 
Sbjct: 328 STTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINT 387

BLAST of Csor.00g036890 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.0e-58
Identity = 120/304 (39.47%), Postives = 177/304 (58.22%), Query Frame = 0

Query: 77  IIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRR 136
           ++ VD    G+ + VQ A+D+VP  ++ +  I +  G YREKV +   K  + + G  R 
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG--RG 149

Query: 137 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSA-PGDKGRQA 196
             +T I  N  A           T  + +  + +  F A  I+F+N      PG+   QA
Sbjct: 150 YQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQA 209

Query: 197 VALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITS 256
           VALRI G++A FY   F G QDTLLDD G H+F +C IQG+IDFIFG  RSLY+ C I S
Sbjct: 210 VALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINS 269

Query: 257 -----TAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIE 316
                T+   G+I A  R S  + +GFSFV C I+GSG +LLGRAWG Y+  ++S  Y+ 
Sbjct: 270 IAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMS 329

Query: 317 DMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFING 375
            +I+P GW++W D ++++T  FG+++C G GA+ K  V + +  T  E   ++D +FI+G
Sbjct: 330 GIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDG 385

BLAST of Csor.00g036890 vs. NCBI nr
Match: KAG6592084.1 (Pectinesterase QRT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 752 bits (1941), Expect = 7.03e-274
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL
Sbjct: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300
           FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS
Sbjct: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300

Query: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360
           RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR
Sbjct: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360

Query: 361 PYVDRNFINGEQWLNL 376
           PYVDRNFINGEQWLNL
Sbjct: 361 PYVDRNFINGEQWLNL 376

BLAST of Csor.00g036890 vs. NCBI nr
Match: XP_022936222.1 (pectinesterase QRT1 [Cucurbita moschata])

HSP 1 Score: 726 bits (1874), Expect = 1.03e-263
Identity = 366/376 (97.34%), Postives = 368/376 (97.87%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MT LPLLLL   LLLFG SD ASARAAAAPAAAV+AAGGGDRYAISWDDLRVDVPSRQGL
Sbjct: 1   MTTLPLLLL---LLLFGPSDPASARAAAAPAAAVLAAGGGDRYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNN RVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNLRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNR+RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300
           FGTARSLYEQCVITSTAETFGAIAAHHRSSP DDTGFSFVRCVINGSGRVLLGRAWGTYS
Sbjct: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYS 300

Query: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360
           RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR
Sbjct: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360

Query: 361 PYVDRNFINGEQWLNL 376
           PYVDRNFINGEQWLNL
Sbjct: 361 PYVDRNFINGEQWLNL 373

BLAST of Csor.00g036890 vs. NCBI nr
Match: XP_022975722.1 (pectinesterase QRT1 [Cucurbita maxima])

HSP 1 Score: 715 bits (1846), Expect = 2.05e-259
Identity = 360/376 (95.74%), Postives = 364/376 (96.81%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MT LPLLLLLL LLLFGLS  AS   AAAPAAA  AAGGG+RYAISWDDLRVDVPSRQGL
Sbjct: 1   MTTLPLLLLLL-LLLFGLSGQASGGGAAAPAAAAFAAGGGERYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGT TRIIVVD++SSGDSTTVQGAVDLVPHNNN RVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNR+RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300
           FGTARSLYEQCVITSTAETFGAIAAHHRSSP DDTGFSFVRCVINGSGRVLLGRAWGTYS
Sbjct: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYS 300

Query: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360
           RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR
Sbjct: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360

Query: 361 PYVDRNFINGEQWLNL 376
           PYVDRNFINGEQWLNL
Sbjct: 361 PYVDRNFINGEQWLNL 375

BLAST of Csor.00g036890 vs. NCBI nr
Match: XP_023536329.1 (pectinesterase QRT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699 bits (1804), Expect = 1.42e-252
Identity = 363/407 (89.19%), Postives = 365/407 (89.68%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MT LPLLLLLLLL   GLSD ASAR AAAPAAAVVA  GG+RYAISWDDLRVDVPSRQGL
Sbjct: 1   MTTLPLLLLLLLL---GLSDQASARGAAAPAAAVVA--GGERYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGT TRIIVVDK+SSGDSTTVQGAVDLVPHNNN RVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTGTRIIVVDKSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYE-------------------------------QCVITSTAETFGAIAAHHRS 300
           FGTARSLYE                               QCVITSTAETFGAIAAHHRS
Sbjct: 241 FGTARSLYEVTVTRSRQVVDPFRVLNLSSDVCLFFSLENQQCVITSTAETFGAIAAHHRS 300

Query: 301 SPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRT 360
           SP DDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRT
Sbjct: 301 SPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRT 360

Query: 361 AVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 376
           AVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL
Sbjct: 361 AVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 402

BLAST of Csor.00g036890 vs. NCBI nr
Match: XP_004150299.1 (pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_015463 [Cucumis sativus])

HSP 1 Score: 557 bits (1436), Expect = 3.20e-197
Identity = 269/332 (81.02%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 45  ISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNH 104
           ISWDDLRVDV  RQ +SS     G+ NGTR  IIVVDK  SGDSTTVQGAVD+VPHNN  
Sbjct: 33  ISWDDLRVDVWRRQRMSSSE---GLGNGTR--IIVVDKNCSGDSTTVQGAVDMVPHNNKQ 92

Query: 105 RVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTA 164
           RVKIYI PGIYREKVYIPITKPYISLIGN+ R+TDTVIT N KASDK + G+ELGTYRTA
Sbjct: 93  RVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTA 152

Query: 165 TVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMG 224
           TVAIDSDYFCA+GITFENTVV+ PGDKGRQ VALRI+G+KAMFYRVKFLG QDTLLDD+G
Sbjct: 153 TVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLG 212

Query: 225 THYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVI 284
           THY+YQCHIQG++DFIFGTARSLYEQCVITSTAE++GAIAAHHR+SP DDTGFSFVRCVI
Sbjct: 213 THYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVI 272

Query: 285 NGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANT 344
           NGSG+V LGRAWG YSRTIYS CYIED+I+PLGWSDW+DPSRQRT  FGQY CRGSGA+T
Sbjct: 273 NGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGAST 332

Query: 345 KGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 376
           K WV WA+TF  EEVRP+VDR +I GEQWLNL
Sbjct: 333 KDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 359

BLAST of Csor.00g036890 vs. ExPASy TrEMBL
Match: A0A6J1F7U7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1)

HSP 1 Score: 726 bits (1874), Expect = 4.97e-264
Identity = 366/376 (97.34%), Postives = 368/376 (97.87%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MT LPLLLL   LLLFG SD ASARAAAAPAAAV+AAGGGDRYAISWDDLRVDVPSRQGL
Sbjct: 1   MTTLPLLLL---LLLFGPSDPASARAAAAPAAAVLAAGGGDRYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNN RVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNLRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNR+RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300
           FGTARSLYEQCVITSTAETFGAIAAHHRSSP DDTGFSFVRCVINGSGRVLLGRAWGTYS
Sbjct: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYS 300

Query: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360
           RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR
Sbjct: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360

Query: 361 PYVDRNFINGEQWLNL 376
           PYVDRNFINGEQWLNL
Sbjct: 361 PYVDRNFINGEQWLNL 373

BLAST of Csor.00g036890 vs. ExPASy TrEMBL
Match: A0A6J1IDU4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 9.91e-260
Identity = 360/376 (95.74%), Postives = 364/376 (96.81%), Query Frame = 0

Query: 1   MTALPLLLLLLLLLLFGLSDHASARAAAAPAAAVVAAGGGDRYAISWDDLRVDVPSRQGL 60
           MT LPLLLLLL LLLFGLS  AS   AAAPAAA  AAGGG+RYAISWDDLRVDVPSRQGL
Sbjct: 1   MTTLPLLLLLL-LLLFGLSGQASGGGAAAPAAAAFAAGGGERYAISWDDLRVDVPSRQGL 60

Query: 61  SSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVY 120
           SSGVHGGGVQNGT TRIIVVD++SSGDSTTVQGAVDLVPHNNN RVKIYIRPGIYREKVY
Sbjct: 61  SSGVHGGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVY 120

Query: 121 IPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180
           IPITKPYISLIGNR+RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF
Sbjct: 121 IPITKPYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITF 180

Query: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240
           ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI
Sbjct: 181 ENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFI 240

Query: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYS 300
           FGTARSLYEQCVITSTAETFGAIAAHHRSSP DDTGFSFVRCVINGSGRVLLGRAWGTYS
Sbjct: 241 FGTARSLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYS 300

Query: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360
           RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR
Sbjct: 301 RTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVR 360

Query: 361 PYVDRNFINGEQWLNL 376
           PYVDRNFINGEQWLNL
Sbjct: 361 PYVDRNFINGEQWLNL 375

BLAST of Csor.00g036890 vs. ExPASy TrEMBL
Match: A0A0A0K706 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1)

HSP 1 Score: 557 bits (1436), Expect = 2.31e-197
Identity = 269/332 (81.02%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 45  ISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNH 104
           ISWDDLRVDV  RQ +SS     G+ NGTR  IIVVDK  SGDSTTVQGAVD+VPHNN  
Sbjct: 44  ISWDDLRVDVWRRQRMSSSE---GLGNGTR--IIVVDKNCSGDSTTVQGAVDMVPHNNKQ 103

Query: 105 RVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTA 164
           RVKIYI PGIYREKVYIPITKPYISLIGN+ R+TDTVIT N KASDK + G+ELGTYRTA
Sbjct: 104 RVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTA 163

Query: 165 TVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMG 224
           TVAIDSDYFCA+GITFENTVV+ PGDKGRQ VALRI+G+KAMFYRVKFLG QDTLLDD+G
Sbjct: 164 TVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLG 223

Query: 225 THYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVI 284
           THY+YQCHIQG++DFIFGTARSLYEQCVITSTAE++GAIAAHHR+SP DDTGFSFVRCVI
Sbjct: 224 THYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVI 283

Query: 285 NGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANT 344
           NGSG+V LGRAWG YSRTIYS CYIED+I+PLGWSDW+DPSRQRT  FGQY CRGSGA+T
Sbjct: 284 NGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGAST 343

Query: 345 KGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 376
           K WV WA+TF  EEVRP+VDR +I GEQWLNL
Sbjct: 344 KDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 370

BLAST of Csor.00g036890 vs. ExPASy TrEMBL
Match: A0A1S3CIB1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 4.39e-197
Identity = 268/332 (80.72%), Postives = 300/332 (90.36%), Query Frame = 0

Query: 45  ISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAVDLVPHNNNH 104
           ISWDDLRVDV  RQ +SSG   G  +NGTR  IIVVDK  SGDSTTVQGAVD+VPHNN  
Sbjct: 51  ISWDDLRVDVWLRQRMSSGEGLG--RNGTR--IIVVDKNCSGDSTTVQGAVDMVPHNNKQ 110

Query: 105 RVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVSGLELGTYRTA 164
           RVKIYI PGIYREK+YIPITKPYISLIGN+ R+T+TVIT N KASDK+++G+ELGTY+TA
Sbjct: 111 RVKIYILPGIYREKLYIPITKPYISLIGNKNRVTETVITWNDKASDKNINGVELGTYKTA 170

Query: 165 TVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMG 224
           TVAIDSDYFCA+GITFENT V+ PGDKGRQ VALRI+G+KA+FYRVKFLG QDTLLDD+G
Sbjct: 171 TVAIDSDYFCATGITFENTNVAKPGDKGRQGVALRITGDKAVFYRVKFLGGQDTLLDDLG 230

Query: 225 THYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDDTGFSFVRCVI 284
           THYFYQCHI+G++DFIFGTARSLYEQCVITSTAE++GAIAAHHR+SP DDTGFSFVRCVI
Sbjct: 231 THYFYQCHIRGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVI 290

Query: 285 NGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANT 344
           NGSG+V LGRAWG YSRTIYS CYIED+I+PLGWSDW+DPSRQRT VFGQY CRGSGANT
Sbjct: 291 NGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVVFGQYNCRGSGANT 350

Query: 345 KGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 376
           K WVPWA+TF+ EEVRP+VDR +I GEQWLNL
Sbjct: 351 KDWVPWAKTFSYEEVRPFVDRKYIKGEQWLNL 378

BLAST of Csor.00g036890 vs. ExPASy TrEMBL
Match: A0A6J1CG92 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1)

HSP 1 Score: 549 bits (1415), Expect = 2.35e-194
Identity = 262/342 (76.61%), Postives = 299/342 (87.43%), Query Frame = 0

Query: 37  AGGG--DRYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGA 96
           AGGG  +RYAI WDDL +D   RQGL+SG +GG       TRIIVVDK  SGDS TVQGA
Sbjct: 25  AGGGRTERYAIGWDDLHIDA-RRQGLNSGDNGG-------TRIIVVDKNCSGDSPTVQGA 84

Query: 97  VDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVS 156
           VDLVPH N  RVKIYI PGIYREKV IP TKPYISLIGN+RR+TDT+IT N KASD+D++
Sbjct: 85  VDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDIN 144

Query: 157 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 216
           G ELGTYRTATV+++SDYFCA+GITFENTVV+ PGD GRQAVALRI+G+KAMFYRVKF+G
Sbjct: 145 GGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIG 204

Query: 217 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDD 276
           QQDTLLDD+GTHYFYQCHIQG++DFIFG ARSLYEQC+I+STAE +GAIAAHHR S  ++
Sbjct: 205 QQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRDSAEEE 264

Query: 277 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 336
           TGFSFV CVINGSG++LLGRAWG YSRTIYSYCYI+D+I+P GWSDW DPSRQRT VFGQ
Sbjct: 265 TGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQ 324

Query: 337 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 376
           Y CRGSGANT+GWVPWA+TF+ EEVRP++D  FINGEQWL+L
Sbjct: 325 YNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 358

BLAST of Csor.00g036890 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 423.7 bits (1088), Expect = 1.5e-118
Identity = 204/342 (59.65%), Postives = 253/342 (73.98%), Query Frame = 0

Query: 45  ISWDDLRVDVPSRQGLSSGV---------HGGGVQNGTRT-RIIVVDKTSSGDSTTVQGA 104
           ISW+DLRV    R   S  +         +     N T   R+IVVDK   GDS TVQGA
Sbjct: 39  ISWEDLRVVEDGRIERSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGA 98

Query: 105 VDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRRMTDTVITSNSKASDKDVS 164
           VD+VP +N+ RVKI+I PGIYREKV +P +KPYIS IGN     DTVI+ + KASD    
Sbjct: 99  VDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCD 158

Query: 165 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 224
           G ELGTYRTA+V+I+SD+FCA+ ITFENTVV+  G++GRQAVALRI G+KA+FYRV+ LG
Sbjct: 159 GKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLG 218

Query: 225 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPHDD 284
            QDTL DD G+HYFYQC+IQG +DFIFG A+SLY+ C I STA+ +GAIAAHHR S  +D
Sbjct: 219 SQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETED 278

Query: 285 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 344
           TGFSFV C I+G+G++ LGRAWG YSRT+YS C+I D+I+P+GWSDW  P RQR  +FG+
Sbjct: 279 TGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGE 338

Query: 345 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 377
           Y CRG GA   G VPW++T T +EV+P++ R FI G+QWL L
Sbjct: 339 YNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

BLAST of Csor.00g036890 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 5.0e-77
Identity = 136/301 (45.18%), Postives = 194/301 (64.45%), Query Frame = 0

Query: 77  IIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRR 136
           + V  K++ GD T +Q A+D +P  N  RV I +  G+Y+EKV IP  K +I++ G    
Sbjct: 85  LTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAE 144

Query: 137 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA 196
              T +     A   D  G  +GTY +A+ A++S +F A  ITF NT  V  PG  G+QA
Sbjct: 145 --KTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQA 204

Query: 197 VALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITS 256
           VALR+S + A F+  + LG QDTL D +G HY+  C+I+G++DFIFG A SLYE C + +
Sbjct: 205 VALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHA 264

Query: 257 TAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISP 316
            A+  GA+ A  RSS  +DTGFSFV+C + G+G + LGRAWG +SR +++Y Y++++I P
Sbjct: 265 IADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILP 324

Query: 317 LGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLN 376
            GW +W DPSR+ T  +GQY+C G+GAN  G V WAR  T EE +P++   FI+G +W+ 
Sbjct: 325 RGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 383

BLAST of Csor.00g036890 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.6e-75
Identity = 138/301 (45.85%), Postives = 187/301 (62.13%), Query Frame = 0

Query: 76  RIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRR 135
           ++I V         +VQ AVD +P NNN  + I I PG YREKV +P TKPYI+  G  R
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 136 RMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQ 195
            +  T I  + +ASD   +G +L TY+TA+V + ++YF A  I+F NT     PG +G Q
Sbjct: 119 DV--TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQ 178

Query: 196 AVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVIT 255
           AVA RISG+KA F    F G QDTL DD G HYF +C+I+G+IDFIFG  RS+Y+ C + 
Sbjct: 179 AVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELH 238

Query: 256 STAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMIS 315
           S A  FG+IAAH R+ P + TGF+FV C + G+G + +GRA G YSR +Y+Y Y + +++
Sbjct: 239 SIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVA 298

Query: 316 PLGWSDWSDPS-RQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQW 375
             GW DW   S + +TA FG Y C G GA     V WAR    E   P++ ++F+NG  W
Sbjct: 299 HGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHW 357

BLAST of Csor.00g036890 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 4.1e-63
Identity = 132/309 (42.72%), Postives = 180/309 (58.25%), Query Frame = 0

Query: 75  TRIIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNR 134
           T  + VDK    + TTVQ AVD V + +  R  I+I  G+Y EKV IP TKP I+L G  
Sbjct: 88  TSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQG-- 147

Query: 135 RRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGR 194
           +    T I  N  A   +      GT+  ATV +    F A  I+F N   +  PGD G 
Sbjct: 148 QGFDITAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGA 207

Query: 195 QAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVI 254
           QAVA+RI+G+++ F    F G QDTL DD G HYF  C+IQG+IDFIFG A+SLY+ C I
Sbjct: 208 QAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRI 267

Query: 255 TSTAETF--------GAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYS 314
            S A           GA+ A+ RSS  +++GFSFV C I G+G V LGRAW  YSR ++ 
Sbjct: 268 ISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFV 327

Query: 315 YCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDR 374
              + D+I+P GW++++DPSR  T  +G+Y C G GA+     P+ +     +V   ++ 
Sbjct: 328 STTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINT 387

BLAST of Csor.00g036890 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 7.3e-60
Identity = 120/304 (39.47%), Postives = 177/304 (58.22%), Query Frame = 0

Query: 77  IIVVDKTSSGDSTTVQGAVDLVPHNNNHRVKIYIRPGIYREKVYIPITKPYISLIGNRRR 136
           ++ VD    G+ + VQ A+D+VP  ++ +  I +  G YREKV +   K  + + G  R 
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG--RG 149

Query: 137 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSA-PGDKGRQA 196
             +T I  N  A           T  + +  + +  F A  I+F+N      PG+   QA
Sbjct: 150 YQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQA 209

Query: 197 VALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITS 256
           VALRI G++A FY   F G QDTLLDD G H+F +C IQG+IDFIFG  RSLY+ C I S
Sbjct: 210 VALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINS 269

Query: 257 -----TAETFGAIAAHHRSSPHDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIE 316
                T+   G+I A  R S  + +GFSFV C I+GSG +LLGRAWG Y+  ++S  Y+ 
Sbjct: 270 IAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMS 329

Query: 317 DMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFING 375
            +I+P GW++W D ++++T  FG+++C G GA+ K  V + +  T  E   ++D +FI+G
Sbjct: 330 GIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDG 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FM792.2e-11759.65Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q8VYZ37.1e-7645.18Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF35.1e-7445.85Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
O230385.8e-6242.72Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2[more]
Q9ZQA31.0e-5839.47Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6592084.17.03e-274100.00Pectinesterase QRT1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022936222.11.03e-26397.34pectinesterase QRT1 [Cucurbita moschata][more]
XP_022975722.12.05e-25995.74pectinesterase QRT1 [Cucurbita maxima][more]
XP_023536329.11.42e-25289.19pectinesterase QRT1 [Cucurbita pepo subsp. pepo][more]
XP_004150299.13.20e-19781.02pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_01546... [more]
Match NameE-valueIdentityDescription
A0A6J1F7U74.97e-26497.34Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1[more]
A0A6J1IDU49.91e-26095.74Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1[more]
A0A0A0K7062.31e-19781.02Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1[more]
A0A1S3CIB14.39e-19780.72Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1[more]
A0A6J1CG922.35e-19476.61Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55590.11.5e-11859.65Pectin lyase-like superfamily protein [more]
AT5G19730.15.0e-7745.18Pectin lyase-like superfamily protein [more]
AT5G47500.13.6e-7545.85Pectin lyase-like superfamily protein [more]
AT1G05310.14.1e-6342.72Pectin lyase-like superfamily protein [more]
AT2G36710.17.3e-6039.47Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 72..376
e-value: 2.4E-100
score: 338.0
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 78..370
e-value: 9.7E-64
score: 214.9
NoneNo IPR availablePANTHERPTHR31321:SF31PECTINESTERASE QRT1coord: 42..376
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 42..376
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 233..242
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 76..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g036890.m01Csor.00g036890.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity