Csor.00g034920 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g034920
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionMalic enzyme
LocationCsor_Chr14: 12353689 .. 12357068 (-)
RNA-Seq ExpressionCsor.00g034920
SyntenyCsor.00g034920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTTCTACACCTTGCTCCACTCCCTCCGCCACCGGAAGACGGCGCCCGGTGGCTCTGAAAGTGGTGTCAATGGACTTGCATGTTCGAGCCGGCGAGAGAAGTGGCAGTGTTGTGGTGGACAACAACAGTGTGAAAGAAATTCCCAATGCTCCGTCGCCGGTGTTTCAAGTGGAACCCAAATCCACCCCTCCCGCCTCCGCCTCCACGGAAGAACAAATCATGACACCATGGACCGTCTCTGTTGCCAGGTGAAGACCGTGTCTGTTCCTGCTCTGACTTGGTTGAACTCTGCAAACCTTTTCATAGCTGAGTTTCTTTCTTTGTGTTGCTTTTTGCTGAAACCTTAGTGGGTATAATTTATTGCGAAATCCACGCCATAACAAAGAGCTTGCGTTTACTGAGAAAGAAAGAGACGCCCATTACTTGCGCGGGCTTCTACCCCCCACAGTTCTTGCTCAAAATGTGCAGGTTAGCCTTCCCTTGTGATACCCTTCAGCCCCATTATAAGTTTTACATTCCTAGTTTGTTATTAACTTTCCAGGTGAAGAAGATGTTGCACAATATCCGTCGATATCAAGTACCATTGCTCAAGTACATGGCAATGATGGATCTTCAGGTACCCATTGTTCATCTCATTTTTTAGTACTAGGACTTCTAAATGATGCTTGAATACAAAATTTATGCATTAGTGAAACTTGTTTTGTTGTACCCTTTTTCAGGAGAGGAATGAAAAGATGTTTTACAAGCTGCTTATAGATCATGTAGAAGAATTACTCCCAGTTGTGTATACACCAACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCATCCACAGGGCCTCTATATCAGTTTGAGAGAAAAGTATAGCTCGTCGATTGCATCGATGCATTTCTTTCTTGGTTTAGGGTGTTGAAATGGTTTTCTTTTGCAGGGGGAAGATCCTTGAGGTCTTGAGGAATTGGCCTGAGAGGAACATTCAAGTCATTGTAGTTACAGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGTCAGGTTAATTCCAATGATGGTTGTCTCTGAAGCTTGAAATTGAGCTATGAACTGATCAAATCCATGTTAATGTTTCAGGGGATGGGCATACCCGTTGGGAAACTCTCTTTATATACTGCACTTGGAGGTGTTCATCCTTCGGTGGTGAGTACCGACATCCTCGTTTTATGATTCATTTCTTATTCTTGTTGGTTCATTTTTTGGGGTTTTTCAGTGCTTGCCAGTGACCATTGATGTTGGGACAAACAATGAGGAGTTGTTGAATGACGAGTTTTACATCGGGCTCAGGCAAAAGAGAGCTACGGGGCAGGTTTGTGATCCCTTCTCTCTCGCCAATGTATCATTTCTAGTTCAATGTGACAAGGCATACTTGGCGAATTCCGAGCTGAGTGGGACGATTTTTCTAAATGTAGGAATATGCTGAACTTATCGATGAATTCATGACTGCAGTCAAGCAGCATTATGGGGAGAAGCTCCTAATTCAGGTATGTTGCTATAACTACACATACTCATTCTGATATTGTTCTAACTCAATTTCCATCGAAGACTTCGCAAACCATAACGCATTCGATTTGCTTGCAAAATACGGCGCAACTCATCTGGTCTTTAATGATGACATTCAGGTTAAAACCCTGGACTTTACTCTTGACTAATTTCAATCAACTGTACCGTAATTGTCATCGTTGAATCTCAGGGGACAGCGTCCGTGGTACTTGCTGGGCTCATTACAGCTCTAAAGTTCGTTGGCGGCTCCTTAGCTGACCATAGATTTTTATTCCTTGGTGCTGGAGAAGTACGTTCATACCGAATCGTGTCTAACATCGAATAAAAGCTCCTCTTTAAGTTTGCGTTGGTTGTTTCAGGCTGGTACTGGAATTGCAGAGCTCATTGCCCTTGAGATATCAAAACAGGTTCATACCCCTTGTTCAGAATTCATTAAGTTAATCTTCATTTTAATTAACAAGAGCTAGTCGTTTGTGTAGACCAATGCTCCGTTGGAAGAGACGCGCAAAAAAGTCTGGCTCGTGGATTCGAAGGTAACTTGTTCGTCAAGCTTGAGAAGGTGTTCATCATTCAGGAAGTTTTTGGAATTTCTGATTCATTTCTTTGTTCCTTCCTCAGGGACTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGGAACTCATTGATGCCGTTAAGGTATCGAATCTTGTAATGTACTTCTACAAATGCATATTCATAATTTGAATCATGTCTTTTACTTACTGTTTCACGTTAGGAAATCAAACCGACTGTCTTGATTGGAACATCGGGCGTTGGAAGAGCATTCACTAAAGAAGTTGTGGAGACTATGACCTCAATCAACGAGGTATTTAATTCGTCCTTTTCACTTTCTGAAGAGCGTATACCGATCTGTTCATGATTTGGGTAACTTGTTGCAGAAACCTATCATTTTCTCACTTTCCAACCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTATAAATGGAGTCAGGTAATACAATGGTTCATTCCCCTCCCTCAAGAACACAATGAATATTCATCTTGTATCACATGGTTGAGTTGTTGCTTTCAGGGTAGTGCCATTTTCGCAAGTGGGAGCCCATTTGATCCAGTTAATTACGAGGGGAAAGTGTTTGTACCTGGCCAGGTCCTCACATTTCAATTCAATTCTAAGCTAAAATCATATCATGCATGATTTGGGTGTTAATATTAATCCTTGGTGATTTATTAACAATTCAGTCAAACAATGCATATATCTTTCCTGGATTCGGTTTGGGGTTAATTATGTCGGGCACAATCCGCGTTCACGATGACTTGCTTCTAGCAGCCTGTAAGTACTCAAAAGCCTATTAAGATTAAAGTATATTAGAGACAGTGCACACATTCTTTCAAAGCTTAGATGAACTAGAGTCCATTGCTATGTTTAAGTCTCGGGTTTCGATCATTACTAATGTACTTGTTCATTACGATCAGCCGAAGCTTTGGCTTCACAAGTGACCCAAGACGACTTCGACAGAGGACTCATCTTTCCTCCATTTGCCAAAATCAGGAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTGAGATTTCAACCTCATTTCCCTTCCACAAAATTTTCAAAGAGTTTGAATCTGCTAATCATTCTCCCATTTCAAATTCTGTCTCATAGGTTTGGCCACTCGCCTCCCTCAACCTAGAGATCTGGTGAAATATGCTGAGGGCTGTATGTATAGTCCTAGCTATCAAAATTACCGGTAG

mRNA sequence

ATGGGTGTTTCTACACCTTGCTCCACTCCCTCCGCCACCGGAAGACGGCGCCCGGTGGCTCTGAAAGTGGTGTCAATGGACTTGCATGTTCGAGCCGGCGAGAGAAGTGGCAGTGTTGTGGTGGACAACAACAGTGTGAAAGAAATTCCCAATGCTCCGTCGCCGGTGTTTCAAGTGGAACCCAAATCCACCCCTCCCGCCTCCGCCTCCACGGAAGAACAAATCATGACACCATGGACCGTCTCTGTTGCCAGTGGGTATAATTTATTGCGAAATCCACGCCATAACAAAGAGCTTGCGTTTACTGAGAAAGAAAGAGACGCCCATTACTTGCGCGGGCTTCTACCCCCCACAGTTCTTGCTCAAAATGTGCAGGTGAAGAAGATGTTGCACAATATCCGTCGATATCAAGTACCATTGCTCAAGTACATGGCAATGATGGATCTTCAGGAGAGGAATGAAAAGATGTTTTACAAGCTGCTTATAGATCATGTAGAAGAATTACTCCCAGTTGTGTATACACCAACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCATCCACAGGGCCTCTATATCAGTTTGAGAGAAAAGGGGAAGATCCTTGAGGTCTTGAGGAATTGGCCTGAGAGGAACATTCAAGTCATTGTAGTTACAGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGTCAGGGGATGGGCATACCCGTTGGGAAACTCTCTTTATATACTGCACTTGGAGGTGTTCATCCTTCGGTGTGCTTGCCAGTGACCATTGATGTTGGGACAAACAATGAGGAGTTGTTGAATGACGAGTTTTACATCGGGCTCAGGCAAAAGAGAGCTACGGGGCAGGAATATGCTGAACTTATCGATGAATTCATGACTGCAGTCAAGCAGCATTATGGGGAGAAGCTCCTAATTCAGGGGACAGCGTCCGTGGTACTTGCTGGGCTCATTACAGCTCTAAAGTTCGTTGGCGGCTCCTTAGCTGACCATAGATTTTTATTCCTTGGTGCTGGAGAAGCTGGTACTGGAATTGCAGAGCTCATTGCCCTTGAGATATCAAAACAGACCAATGCTCCGTTGGAAGAGACGCGCAAAAAAGTCTGGCTCGTGGATTCGAAGGGACTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGGAACTCATTGATGCCGTTAAGGAAATCAAACCGACTGTCTTGATTGGAACATCGGGCGTTGGAAGAGCATTCACTAAAGAAGTTGTGGAGACTATGACCTCAATCAACGAGAAACCTATCATTTTCTCACTTTCCAACCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTATAAATGGAGTCAGGGTAGTGCCATTTTCGCAAGTGGGAGCCCATTTGATCCAGTTAATTACGAGGGGAAAGTGTTTGTACCTGGCCAGTCAAACAATGCATATATCTTTCCTGGATTCGGTTTGGGGTTAATTATGTCGGGCACAATCCGCGTTCACGATGACTTGCTTCTAGCAGCCTCCGAAGCTTTGGCTTCACAAGTGACCCAAGACGACTTCGACAGAGGACTCATCTTTCCTCCATTTGCCAAAATCAGGAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAGAGATCTGGTGAAATATGCTGAGGGCTGTATGTATAGTCCTAGCTATCAAAATTACCGGTAG

Coding sequence (CDS)

ATGGGTGTTTCTACACCTTGCTCCACTCCCTCCGCCACCGGAAGACGGCGCCCGGTGGCTCTGAAAGTGGTGTCAATGGACTTGCATGTTCGAGCCGGCGAGAGAAGTGGCAGTGTTGTGGTGGACAACAACAGTGTGAAAGAAATTCCCAATGCTCCGTCGCCGGTGTTTCAAGTGGAACCCAAATCCACCCCTCCCGCCTCCGCCTCCACGGAAGAACAAATCATGACACCATGGACCGTCTCTGTTGCCAGTGGGTATAATTTATTGCGAAATCCACGCCATAACAAAGAGCTTGCGTTTACTGAGAAAGAAAGAGACGCCCATTACTTGCGCGGGCTTCTACCCCCCACAGTTCTTGCTCAAAATGTGCAGGTGAAGAAGATGTTGCACAATATCCGTCGATATCAAGTACCATTGCTCAAGTACATGGCAATGATGGATCTTCAGGAGAGGAATGAAAAGATGTTTTACAAGCTGCTTATAGATCATGTAGAAGAATTACTCCCAGTTGTGTATACACCAACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCATCCACAGGGCCTCTATATCAGTTTGAGAGAAAAGGGGAAGATCCTTGAGGTCTTGAGGAATTGGCCTGAGAGGAACATTCAAGTCATTGTAGTTACAGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGTCAGGGGATGGGCATACCCGTTGGGAAACTCTCTTTATATACTGCACTTGGAGGTGTTCATCCTTCGGTGTGCTTGCCAGTGACCATTGATGTTGGGACAAACAATGAGGAGTTGTTGAATGACGAGTTTTACATCGGGCTCAGGCAAAAGAGAGCTACGGGGCAGGAATATGCTGAACTTATCGATGAATTCATGACTGCAGTCAAGCAGCATTATGGGGAGAAGCTCCTAATTCAGGGGACAGCGTCCGTGGTACTTGCTGGGCTCATTACAGCTCTAAAGTTCGTTGGCGGCTCCTTAGCTGACCATAGATTTTTATTCCTTGGTGCTGGAGAAGCTGGTACTGGAATTGCAGAGCTCATTGCCCTTGAGATATCAAAACAGACCAATGCTCCGTTGGAAGAGACGCGCAAAAAAGTCTGGCTCGTGGATTCGAAGGGACTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGGAACTCATTGATGCCGTTAAGGAAATCAAACCGACTGTCTTGATTGGAACATCGGGCGTTGGAAGAGCATTCACTAAAGAAGTTGTGGAGACTATGACCTCAATCAACGAGAAACCTATCATTTTCTCACTTTCCAACCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTATAAATGGAGTCAGGGTAGTGCCATTTTCGCAAGTGGGAGCCCATTTGATCCAGTTAATTACGAGGGGAAAGTGTTTGTACCTGGCCAGTCAAACAATGCATATATCTTTCCTGGATTCGGTTTGGGGTTAATTATGTCGGGCACAATCCGCGTTCACGATGACTTGCTTCTAGCAGCCTCCGAAGCTTTGGCTTCACAAGTGACCCAAGACGACTTCGACAGAGGACTCATCTTTCCTCCATTTGCCAAAATCAGGAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAGAGATCTGGTGAAATATGCTGAGGGCTGTATGTATAGTCCTAGCTATCAAAATTACCGGTAG

Protein sequence

MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVEPKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYGEKLLIQGTASVVLAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDLVKYAEGCMYSPSYQNYR
Homology
BLAST of Csor.00g034920 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 2.1e-252
Identity = 444/637 (69.70%), Postives = 521/637 (81.79%), Query Frame = 0

Query: 2   GVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVEP 61
           GVS   S       RRP+ + +V+ +      ERS  V VD  +VK++ NAP  V   + 
Sbjct: 16  GVSWTQSQSVRLSVRRPMVVAMVNSN---GRPERSVGVSVD-GAVKDV-NAPVAVEVADS 75

Query: 62  KSTPPAS-------------ASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDA 121
           +S  P +             ++TE+  +TPW+VSVASGY+LLR+P HNK LAFTEKERDA
Sbjct: 76  ESKKPTAVVGGGVEDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDA 135

Query: 122 HYLRGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEEL 181
           HYLRGLLPP V+  ++QVKKM+HNIR+Y+VPL +Y AMMDLQERNE++FYKLLI+++EEL
Sbjct: 136 HYLRGLLPPVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEEL 195

Query: 182 LPVVYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERIL 241
           LP+VYTPTVGEACQKYG+IF++PQGLYISL++KGK+LE+L+NWP++ IQVIVVTDGERIL
Sbjct: 196 LPIVYTPTVGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERIL 255

Query: 242 GLGDLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRAT 301
           GLGDLGCQGMGIPVGKLSLYTALGG+ PS CLP+TIDVGTNNE++LNDEFYIGLRQ+RA+
Sbjct: 256 GLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRAS 315

Query: 302 GQEYAELIDEFMTAVKQHYGEKLL----------------------------IQGTASVV 361
           G+EYAEL++EFM+AVKQ+YGEK+L                            IQGTASVV
Sbjct: 316 GKEYAELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVV 375

Query: 362 LAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDS 421
           LAGLI+ALK VGGSLADH+FLFLGAGEAGTGIAELIALEISKQTNAPLEETRKK+WLVDS
Sbjct: 376 LAGLISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDS 435

Query: 422 KGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMT 481
           KGLIV SR D+LQHFKKPWAH+H+P+ + +DAVK IKPTVLIG+SG G+ FTKEVVE M+
Sbjct: 436 KGLIVRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMS 495

Query: 482 SINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAY 541
           S NEKPII +LSNPTSQSECTAE+AY WS+G  IFASGSPF PV Y GKV+V GQSNNAY
Sbjct: 496 SFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAY 555

Query: 542 IFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAAS 598
           IFPGFGLGLI+SG IRVHD++LLAASEALA QVTQ+ FD GLI+PPF  IRKISAHIAA 
Sbjct: 556 IFPGFGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAK 615

BLAST of Csor.00g034920 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.2e-250
Identity = 430/590 (72.88%), Postives = 504/590 (85.42%), Query Frame = 0

Query: 44  NSVKEIPNAPSPVFQVEPKSTPPASA--------STEEQIMTPWTVSVASGYNLLRNPRH 103
           +++K+I +  S V  ++PK+T             +TE+Q++TPWTVSVASGY+LLR+PRH
Sbjct: 3   STLKDIRDGAS-VLDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDPRH 62

Query: 104 NKELAFTEKERDAHYLRGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEK 163
           NK LAF +KERDAHYL GLLPP V  Q +Q +K++++IR+YQVPL KYMAMMDLQERNE+
Sbjct: 63  NKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERNER 122

Query: 164 MFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERN 223
           +FYKLLID+VEELLPVVYTPTVGEACQKYGSIFR PQGLYISL+EKGKILEVL+NWPER 
Sbjct: 123 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERR 182

Query: 224 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLN 283
           IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGV PS CLP+TIDVGTNNE+LL 
Sbjct: 183 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLA 242

Query: 284 DEFYIGLRQKRATGQEYAELIDEFMTAVKQHYGEKLL----------------------- 343
           +EFYIGL+Q+RATG+EY+E + EFM+ VKQ+YGEK+L                       
Sbjct: 243 NEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTHL 302

Query: 344 -----IQGTASVVLAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAP 403
                IQGTASVVLAG+++AL+ +GG+LADH+FLFLGAGEAGTGIAELIALE+SKQT  P
Sbjct: 303 AFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTKCP 362

Query: 404 LEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGV 463
           +EETRKK+WLVDSKGLIV SRKD+LQ FKKPWAHEH+P+K+L+DAVK IKPTVLIG+SGV
Sbjct: 363 IEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSSGV 422

Query: 464 GRAFTKEVVETMTSINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYE 523
           G+AFTKEV+E M S NEKP+I +LSNPTSQSECTAEEAY W+QG AIFASGSPFDPV Y 
Sbjct: 423 GKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVEYN 482

Query: 524 GKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPF 583
           GK FVPGQ+NNAYIFPG G+GL++SG IRVHD++LLAASEALA QVTQ++FD+GLI+PPF
Sbjct: 483 GKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYPPF 542

Query: 584 AKIRKISAHIAASVAAKAYELGLATRLPQPRDLVKYAEGCMYSPSYQNYR 598
           + IRKISAHIAA+VAAKAYELGLATRLPQP +LVKYAE CMYSP Y++YR
Sbjct: 543 SNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSYR 591

BLAST of Csor.00g034920 vs. ExPASy Swiss-Prot
Match: Q9CA83 (NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NADP-ME4 PE=1 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 2.6e-250
Identity = 439/630 (69.68%), Postives = 516/630 (81.90%), Query Frame = 0

Query: 7   CSTPSATGRRRPVALKVVSMDLHVRAG---ERSGSVVVDNNSVKEIPNAPSPVFQVEPKS 66
           CST  +  + R +     S+ +    G   + +GSV+++  +     +  +    + PKS
Sbjct: 18  CSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIETTATSS-SSLETSAADIVPKS 77

Query: 67  TPPA--------SASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLL 126
           T            A+TE+  +TPW++SVASGY LLR+P HNK LAF+ +ERDAHYLRGLL
Sbjct: 78  TVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLL 137

Query: 127 PPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTP 186
           PPTV++Q++QVKK++H +R+YQVPL KYMAMMDLQE NE++FYKLLIDHVEELLPV+YTP
Sbjct: 138 PPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTP 197

Query: 187 TVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGC 246
           TVGEACQKYGSIF  PQGL+ISL+EKGKI EVLRNWPE+NIQVIVVTDGERILGLGDLGC
Sbjct: 198 TVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGC 257

Query: 247 QGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAEL 306
           QGMGIPVGKLSLYTALGGV PS CLPVTIDVGTNNE+LLNDEFYIGLRQ+RATG+EY+EL
Sbjct: 258 QGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSEL 317

Query: 307 IDEFMTAVKQHYGEKLL----------------------------IQGTASVVLAGLITA 366
           + EFMTAVKQ+YGEK++                            IQGTASVVLAGLI A
Sbjct: 318 MHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAA 377

Query: 367 LKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSS 426
           L+FVGGSL+DHRFLFLGAGEAGTGIAELIALEISK+++ PLEE RK +WLVDSKGLIVSS
Sbjct: 378 LRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSS 437

Query: 427 RKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPI 486
           RK+++QHFKKPWAH+H+PI+EL+DAVK IKPTVLIGTSGVG+ FT++VVETM  +NEKPI
Sbjct: 438 RKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPI 497

Query: 487 IFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGL 546
           I SLSNPTSQSECTAEEAY WSQG AIFASGSPF PV YEGK FVPGQ+NNAYIFPGFGL
Sbjct: 498 ILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGL 557

Query: 547 GLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYE 598
           GLIMSGTIRVHDD+LLAASEALA ++ ++ +++G+I+PPF  IRKISA IAA VAAKAYE
Sbjct: 558 GLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYE 617

BLAST of Csor.00g034920 vs. ExPASy Swiss-Prot
Match: P43279 (NADP-dependent malic enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ME6 PE=2 SV=2)

HSP 1 Score: 863.6 bits (2230), Expect = 1.3e-249
Identity = 421/556 (75.72%), Postives = 484/556 (87.05%), Query Frame = 0

Query: 70  STEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKKM 129
           +TEE  +TPW  SVASGY LLR+P HNK LAF+EKERDAHYLRGLLPP V++Q++QVKK+
Sbjct: 84  ATEEVPVTPWAFSVASGYTLLRDPHHNKGLAFSEKERDAHYLRGLLPPAVVSQDLQVKKI 143

Query: 130 LHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFR 189
           +HN+R+Y VPL +YMAMMDLQERNE++FYKLLID+VEELLPVVYTPTVGEACQKYGSIFR
Sbjct: 144 MHNLRQYSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFR 203

Query: 190 HPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 249
            PQGLY+SL++KGK+L+VLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 204 QPQGLYVSLKDKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 263

Query: 250 ALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYGE 309
           ALGGV PS CLP+TIDVGTNNE+LLNDEFYIGLRQ+RATG+EY EL++EFM+AVKQ YGE
Sbjct: 264 ALGGVRPSACLPITIDVGTNNEQLLNDEFYIGLRQRRATGKEYHELMEEFMSAVKQIYGE 323

Query: 310 KLL----------------------------IQGTASVVLAGLITALKFVGGSLADHRFL 369
           K+L                            IQGTASVVLAGL+++LK VGG+LA+H +L
Sbjct: 324 KVLIQFEDFANHNAFDLLAKYSKSHLVFNDDIQGTASVVLAGLLSSLKVVGGTLAEHTYL 383

Query: 370 FLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAH 429
           FLGAGEAGTGIAELIALEISKQT AP+EE RKKVWL+DSKGLIV+SRK++LQ FKKPWAH
Sbjct: 384 FLGAGEAGTGIAELIALEISKQTKAPIEECRKKVWLLDSKGLIVNSRKESLQAFKKPWAH 443

Query: 430 EHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSECT 489
           EH+P+  L+DAV+ IKPTVLIGTSGVG+ FTKEV+E M S NE+P+IFSLSNPTS SECT
Sbjct: 444 EHEPVTTLLDAVQSIKPTVLIGTSGVGKTFTKEVIEAMASFNERPVIFSLSNPTSHSECT 503

Query: 490 AEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDL 549
           AEEAY WSQG A+FASGSPFDPV Y GK+ VPGQSNNAYIFPGFGLG+++SG +RVH+D+
Sbjct: 504 AEEAYNWSQGRAVFASGSPFDPVEYNGKIHVPGQSNNAYIFPGFGLGVVISGAVRVHEDM 563

Query: 550 LLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDLV 598
           LLAASE LA Q TQ++F++G IFPPF  IRKISA IAASVAAKAYELGLATRLPQPRDL 
Sbjct: 564 LLAASETLADQATQENFEKGSIFPPFTNIRKISARIAASVAAKAYELGLATRLPQPRDLE 623

BLAST of Csor.00g034920 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 2.1e-247
Identity = 420/557 (75.40%), Postives = 488/557 (87.61%), Query Frame = 0

Query: 69  ASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKK 128
           ++TE+ ++TPWT SVASG +LLR+PR+NK LAFTE ERDAHYLRGLLPP+V  Q +Q K+
Sbjct: 33  SATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTEGERDAHYLRGLLPPSVFNQELQEKR 92

Query: 129 MLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIF 188
           ++HN+R+Y+VPL +YMA+MDLQERNE++FYKLLID+V ELLPVVYTPTVGEACQKYGSIF
Sbjct: 93  LMHNLRQYEVPLHRYMALMDLQERNERLFYKLLIDNVAELLPVVYTPTVGEACQKYGSIF 152

Query: 189 RHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 248
           R PQGLYISL+EKGKILEVL+NWPE++IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 153 RRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 212

Query: 249 TALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYG 308
           TALGGV PS CLPVTIDVGTNNE+LLNDEFYIGLRQ+RATGQEYA  +DEFM AVKQ+YG
Sbjct: 213 TALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRRATGQEYATFLDEFMRAVKQNYG 272

Query: 309 EKLL----------------------------IQGTASVVLAGLITALKFVGGSLADHRF 368
           EK+L                            IQGTASVVLAGL+ +LK VGG+LADH F
Sbjct: 273 EKVLVQFEDFANHNAFDLLEKYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTF 332

Query: 369 LFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWA 428
           LFLGAGEAGTGIAELIA+E+SKQT AP+EETRKK+WLVDSKGLIVSSR ++LQ FKKPWA
Sbjct: 333 LFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKIWLVDSKGLIVSSRLESLQQFKKPWA 392

Query: 429 HEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSEC 488
           HEH+P+K L++AVK IKPTVLIG+SG G+ FTKEVVETM S+NEKP+I +LSNPTSQSEC
Sbjct: 393 HEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPLILALSNPTSQSEC 452

Query: 489 TAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDD 548
           TAEEAY WS+G AIFASGSPFDPV YEGK+FVPGQ+NNAYIFPGFGLGLIMSG IRV D+
Sbjct: 453 TAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGLGLIMSGAIRVRDE 512

Query: 549 LLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDL 598
           +LLAASEALA+QV+++++D+GLI+PPF  IRKISA+IAA VAAKAY+LGLA+ L +P+DL
Sbjct: 513 MLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAAKVAAKAYDLGLASHLKRPKDL 572

BLAST of Csor.00g034920 vs. NCBI nr
Match: KAG6581878.1 (NADP-dependent malic enzyme, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1181 bits (3056), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE
Sbjct: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA
Sbjct: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300

Query: 301 TAVKQHYGEKLLIQGTASVVLAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEI 360
           TAVKQHYGEKLLIQGTASVVLAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEI
Sbjct: 301 TAVKQHYGEKLLIQGTASVVLAGLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEI 360

Query: 361 SKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTV 420
           SKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTV
Sbjct: 361 SKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTV 420

Query: 421 LIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSP 480
           LIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSP
Sbjct: 421 LIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSP 480

Query: 481 FDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDR 540
           FDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDR
Sbjct: 481 FDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDR 540

Query: 541 GLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDLVKYAEGCMYSPSYQNYR 597
           GLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDLVKYAEGCMYSPSYQNYR
Sbjct: 541 GLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDLVKYAEGCMYSPSYQNYR 597

BLAST of Csor.00g034920 vs. NCBI nr
Match: KAG7018314.1 (NADP-dependent malic enzyme, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1163 bits (3009), Expect = 0.0
Identity = 596/625 (95.36%), Postives = 596/625 (95.36%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE
Sbjct: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 61  PKSTPAASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA
Sbjct: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 301 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 360

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL
Sbjct: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS
Sbjct: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS
Sbjct: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 600

BLAST of Csor.00g034920 vs. NCBI nr
Match: XP_022956197.1 (NADP-dependent malic enzyme-like [Cucurbita moschata])

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 594/625 (95.04%), Postives = 595/625 (95.20%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMD HVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE
Sbjct: 11  MGVSTPCSTPSATGRRRPVALKVVSMDSHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 70

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 71  PKSTPAASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 130

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA
Sbjct: 131 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 190

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 191 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 250

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 251 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 310

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 311 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 370

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL
Sbjct: 371 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 430

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS
Sbjct: 431 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 490

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY+GKVFVPGQSNNAYIFPGFGLGLIMS
Sbjct: 491 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYKGKVFVPGQSNNAYIFPGFGLGLIMS 550

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 551 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 610

BLAST of Csor.00g034920 vs. NCBI nr
Match: XP_022979251.1 (NADP-dependent malic enzyme-like [Cucurbita maxima])

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 589/625 (94.24%), Postives = 594/625 (95.04%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVF+VE
Sbjct: 11  MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFEVE 70

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPW+VSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 71  PKSTPAASASTEEQIMTPWSVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 130

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFY+LLIDHVEELLPVVYTPTVGEA
Sbjct: 131 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYRLLIDHVEELLPVVYTPTVGEA 190

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 191 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 250

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 251 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 310

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 311 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 370

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIV SRKDTL
Sbjct: 371 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVISRKDTL 430

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETM SINEKPIIFSLS
Sbjct: 431 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMASINEKPIIFSLS 490

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY+GKVFVPGQSNNAYIFPGFGLGLIMS
Sbjct: 491 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYKGKVFVPGQSNNAYIFPGFGLGLIMS 550

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDF+RGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 551 GTIRVHDDLLLAASEALASQVTQDDFNRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 610

BLAST of Csor.00g034920 vs. NCBI nr
Match: XP_023527958.1 (NADP-dependent malic enzyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1150 bits (2974), Expect = 0.0
Identity = 587/625 (93.92%), Postives = 593/625 (94.88%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAG+RSGSVVVDNNSVKEIPNAPSPVF+VE
Sbjct: 11  MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGDRSGSVVVDNNSVKEIPNAPSPVFEVE 70

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 71  PKSTPAASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 130

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQ+KKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA
Sbjct: 131 AQNVQLKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 190

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 191 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 250

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 251 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 310

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 311 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 370

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIA LIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL
Sbjct: 371 GSLADHRFLFLGAGEAGTGIAALIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 430

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPT+LIGTSGVGRAFTKEVVETM SINEKPIIFSLS
Sbjct: 431 QHFKKPWAHEHKPIKELIDAVKEIKPTILIGTSGVGRAFTKEVVETMASINEKPIIFSLS 490

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY+GK FVPGQSNNAYIFPGFGLGLIMS
Sbjct: 491 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYKGKEFVPGQSNNAYIFPGFGLGLIMS 550

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 551 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 610

BLAST of Csor.00g034920 vs. ExPASy TrEMBL
Match: A0A6J1GVN6 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111457964 PE=3 SV=1)

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 594/625 (95.04%), Postives = 595/625 (95.20%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMD HVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE
Sbjct: 11  MGVSTPCSTPSATGRRRPVALKVVSMDSHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 70

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 71  PKSTPAASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 130

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA
Sbjct: 131 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 190

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 191 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 250

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 251 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 310

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 311 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 370

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL
Sbjct: 371 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 430

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS
Sbjct: 431 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 490

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY+GKVFVPGQSNNAYIFPGFGLGLIMS
Sbjct: 491 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYKGKVFVPGQSNNAYIFPGFGLGLIMS 550

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 551 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 610

BLAST of Csor.00g034920 vs. ExPASy TrEMBL
Match: A0A6J1IQ94 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479032 PE=3 SV=1)

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 589/625 (94.24%), Postives = 594/625 (95.04%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVF+VE
Sbjct: 11  MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFEVE 70

Query: 61  PKSTPPASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 120
           PKSTP ASASTEEQIMTPW+VSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL
Sbjct: 71  PKSTPAASASTEEQIMTPWSVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVL 130

Query: 121 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEA 180
           AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFY+LLIDHVEELLPVVYTPTVGEA
Sbjct: 131 AQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYRLLIDHVEELLPVVYTPTVGEA 190

Query: 181 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 240
           CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI
Sbjct: 191 CQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGI 250

Query: 241 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 300
           PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM
Sbjct: 251 PVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFM 310

Query: 301 TAVKQHYGEKLLIQ----------------------------GTASVVLAGLITALKFVG 360
           TAVKQHYGEKLLIQ                            GTASVVLAGLITALKFVG
Sbjct: 311 TAVKQHYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAGLITALKFVG 370

Query: 361 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTL 420
           GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIV SRKDTL
Sbjct: 371 GSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVISRKDTL 430

Query: 421 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLS 480
           QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETM SINEKPIIFSLS
Sbjct: 431 QHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMASINEKPIIFSLS 490

Query: 481 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMS 540
           NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY+GKVFVPGQSNNAYIFPGFGLGLIMS
Sbjct: 491 NPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYKGKVFVPGQSNNAYIFPGFGLGLIMS 550

Query: 541 GTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 597
           GTIRVHDDLLLAASEALASQVTQDDF+RGLIFPPFAKIRKISAHIAASVAAKAYELGLAT
Sbjct: 551 GTIRVHDDLLLAASEALASQVTQDDFNRGLIFPPFAKIRKISAHIAASVAAKAYELGLAT 610

BLAST of Csor.00g034920 vs. ExPASy TrEMBL
Match: A0A6J1DJF8 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1)

HSP 1 Score: 1053 bits (2722), Expect = 0.0
Identity = 538/634 (84.86%), Postives = 567/634 (89.43%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVS  CSTPS  GRRRP ALKV+SMD + RAGER GSVVV+NN+VKEI  APSPV +VE
Sbjct: 13  MGVSGACSTPSGNGRRRPAALKVLSMDSNGRAGERGGSVVVENNTVKEIAKAPSPVVEVE 72

Query: 61  PKSTPPA---------SASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYL 120
           PKST            +A+TEEQIMTPW+VSVASGYNLLR+PRHNK LAFTEKERDAHYL
Sbjct: 73  PKSTARGWAQDAYVEDAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYL 132

Query: 121 RGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPV 180
           RGLLPPTV+AQ++QVKKMLHNIR+YQVPL KYMAMMDLQERNEK+FYKLLIDHVEELLPV
Sbjct: 133 RGLLPPTVVAQDLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPV 192

Query: 181 VYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLG 240
           VYTPTVGEACQKYGSIFR PQGLYISLREKGKILEVLRNWPE+NIQVIVVTDGERILGLG
Sbjct: 193 VYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLG 252

Query: 241 DLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQE 300
           DLGCQGMGIPVGKLSLYTALGGV PS CLPVTIDVGTNNEELLNDEFYIGLRQKRATGQE
Sbjct: 253 DLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQE 312

Query: 301 YAELIDEFMTAVKQHYGEKLLIQ----------------------------GTASVVLAG 360
           YAELIDEFMTAVKQ YGEKLLIQ                            GTASVVLAG
Sbjct: 313 YAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLAG 372

Query: 361 LITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGL 420
           L+ ALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKK+WLVDSKGL
Sbjct: 373 LVAALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGL 432

Query: 421 IVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSIN 480
           IV+SRK+TLQHFKKPWAHEHKPIK+L+DAVKEI+PT+LIGTSGVGR FTKEVVETM SIN
Sbjct: 433 IVNSRKETLQHFKKPWAHEHKPIKKLVDAVKEIRPTILIGTSGVGRTFTKEVVETMASIN 492

Query: 481 EKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFP 540
           EKPII SLSNPTSQSECTAEEAYKWSQG AIFASGSPFDPV Y+GKVFVPGQ+NNAYIFP
Sbjct: 493 EKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFP 552

Query: 541 GFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAA 597
           GFGLGLIMSGTIRVHDD+LLAASEALASQVTQDDFD+GLI+PPF  IRKISAHIAASVAA
Sbjct: 553 GFGLGLIMSGTIRVHDDMLLAASEALASQVTQDDFDKGLIYPPFTNIRKISAHIAASVAA 612

BLAST of Csor.00g034920 vs. ExPASy TrEMBL
Match: A0A1S4DWR5 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1)

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 535/635 (84.25%), Postives = 569/635 (89.61%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVS  CSTPS TG RRP+ALKVVSMD + RAGERSGSV+V+N++VKEI   PSPV +VE
Sbjct: 13  MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 72

Query: 61  PKSTP----------PASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHY 120
           PKST            ++A+ +EQIMTPW+VSVASGYNLLR+PRHNK LAFTEKERDAHY
Sbjct: 73  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLP 180
           LRGLLPPT++AQN+QVKKMLHNIR+YQVPL KYMAMMDLQERNEK+FYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFR PQGLYISLREKG+ILEVLRNWPE+NIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGV PS CLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQHYGEKLLIQ----------------------------GTASVVLA 360
           EYAEL+DEFMTAVKQ YGEKLLIQ                            GTASVVLA
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 372

Query: 361 GLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
           GLI+ALK VGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSI 480
           LIVSSRKDTLQHFKKPWAHEHKPIK+LIDAVKEIKPTVLIGTSGVGR FTKEVVETM SI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIF 540
           NE+PII SLSNPTSQSECTAEEAYKWSQG AIFASGSPFDPV ++GK+FVPGQ+NNAYIF
Sbjct: 493 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 552

Query: 541 PGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVA 597
           PGFGLGLIMSGTIRVHDD+LLAASEALASQV+Q+DFD+GLI+PPF  IRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 612

BLAST of Csor.00g034920 vs. ExPASy TrEMBL
Match: A0A5D3CAD9 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 PE=3 SV=1)

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 535/635 (84.25%), Postives = 569/635 (89.61%), Query Frame = 0

Query: 1   MGVSTPCSTPSATGRRRPVALKVVSMDLHVRAGERSGSVVVDNNSVKEIPNAPSPVFQVE 60
           MGVS  CSTPS TG RRP+ALKVVSMD + RAGERSGSV+V+N++VKEI   PSPV +VE
Sbjct: 1   MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 60

Query: 61  PKSTP----------PASASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHY 120
           PKST            ++A+ +EQIMTPW+VSVASGYNLLR+PRHNK LAFTEKERDAHY
Sbjct: 61  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120

Query: 121 LRGLLPPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLP 180
           LRGLLPPT++AQN+QVKKMLHNIR+YQVPL KYMAMMDLQERNEK+FYKLLIDHVEELLP
Sbjct: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180

Query: 181 VVYTPTVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFR PQGLYISLREKG+ILEVLRNWPE+NIQVIVVTDGERILGL
Sbjct: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 240

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGV PS CLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300

Query: 301 EYAELIDEFMTAVKQHYGEKLLIQ----------------------------GTASVVLA 360
           EYAEL+DEFMTAVKQ YGEKLLIQ                            GTASVVLA
Sbjct: 301 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 360

Query: 361 GLITALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
           GLI+ALK VGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 361 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSI 480
           LIVSSRKDTLQHFKKPWAHEHKPIK+LIDAVKEIKPTVLIGTSGVGR FTKEVVETM SI
Sbjct: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480

Query: 481 NEKPIIFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIF 540
           NE+PII SLSNPTSQSECTAEEAYKWSQG AIFASGSPFDPV ++GK+FVPGQ+NNAYIF
Sbjct: 481 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 540

Query: 541 PGFGLGLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVA 597
           PGFGLGLIMSGTIRVHDD+LLAASEALASQV+Q+DFD+GLI+PPF  IRKISAHIAASVA
Sbjct: 541 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 600

BLAST of Csor.00g034920 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 865.9 bits (2236), Expect = 1.9e-251
Identity = 439/630 (69.68%), Postives = 516/630 (81.90%), Query Frame = 0

Query: 7   CSTPSATGRRRPVALKVVSMDLHVRAG---ERSGSVVVDNNSVKEIPNAPSPVFQVEPKS 66
           CST  +  + R +     S+ +    G   + +GSV+++  +     +  +    + PKS
Sbjct: 18  CSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIETTATSS-SSLETSAADIVPKS 77

Query: 67  TPPA--------SASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLL 126
           T            A+TE+  +TPW++SVASGY LLR+P HNK LAF+ +ERDAHYLRGLL
Sbjct: 78  TVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLL 137

Query: 127 PPTVLAQNVQVKKMLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTP 186
           PPTV++Q++QVKK++H +R+YQVPL KYMAMMDLQE NE++FYKLLIDHVEELLPV+YTP
Sbjct: 138 PPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTP 197

Query: 187 TVGEACQKYGSIFRHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGC 246
           TVGEACQKYGSIF  PQGL+ISL+EKGKI EVLRNWPE+NIQVIVVTDGERILGLGDLGC
Sbjct: 198 TVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGC 257

Query: 247 QGMGIPVGKLSLYTALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAEL 306
           QGMGIPVGKLSLYTALGGV PS CLPVTIDVGTNNE+LLNDEFYIGLRQ+RATG+EY+EL
Sbjct: 258 QGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSEL 317

Query: 307 IDEFMTAVKQHYGEKLL----------------------------IQGTASVVLAGLITA 366
           + EFMTAVKQ+YGEK++                            IQGTASVVLAGLI A
Sbjct: 318 MHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAA 377

Query: 367 LKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSS 426
           L+FVGGSL+DHRFLFLGAGEAGTGIAELIALEISK+++ PLEE RK +WLVDSKGLIVSS
Sbjct: 378 LRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSS 437

Query: 427 RKDTLQHFKKPWAHEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPI 486
           RK+++QHFKKPWAH+H+PI+EL+DAVK IKPTVLIGTSGVG+ FT++VVETM  +NEKPI
Sbjct: 438 RKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPI 497

Query: 487 IFSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGL 546
           I SLSNPTSQSECTAEEAY WSQG AIFASGSPF PV YEGK FVPGQ+NNAYIFPGFGL
Sbjct: 498 ILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGL 557

Query: 547 GLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYE 598
           GLIMSGTIRVHDD+LLAASEALA ++ ++ +++G+I+PPF  IRKISA IAA VAAKAYE
Sbjct: 558 GLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYE 617

BLAST of Csor.00g034920 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 839.7 bits (2168), Expect = 1.4e-243
Identity = 410/557 (73.61%), Postives = 478/557 (85.82%), Query Frame = 0

Query: 69  ASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKK 128
           ++T +Q++TPW  SVASGY L+R+PR+NK LAFT+KERDAHY+ GLLPP VL+Q+VQ +K
Sbjct: 32  SATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERK 91

Query: 129 MLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIF 188
           ++HN+R+Y VPL +YMA+MDLQERNE++FYKLLID+VEELLPVVYTPTVGEACQKYGSI+
Sbjct: 92  VMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIY 151

Query: 189 RHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 248
           R PQGLYISL+EKGKILEVL+NWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 152 RRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 211

Query: 249 TALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYG 308
           TALGG+ PS CLP+TIDVGTNNE+LLN+EFYIGL+QKRA G+EYAE + EFM AVKQ+YG
Sbjct: 212 TALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYG 271

Query: 309 EKLL----------------------------IQGTASVVLAGLITALKFVGGSLADHRF 368
           EK+L                            IQGTASVVLAGLI A K +G SLADH F
Sbjct: 272 EKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTF 331

Query: 369 LFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWA 428
           LFLGAGEAGTGIAELIAL+ISK+T  P++ETRKK+WLVDSKGLIVS RK++LQHFK+PWA
Sbjct: 332 LFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWA 391

Query: 429 HEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSEC 488
           H+HKP+KEL+ AV  IKPTVLIGTSGVG+ FTKEVVE M ++NEKP+I +LSNPTSQ+EC
Sbjct: 392 HDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAEC 451

Query: 489 TAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDD 548
           TAEEAY W++G AIFASGSPFDPV Y+GK F PGQ+NN YIFPG GLGLIMSG IRV DD
Sbjct: 452 TAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDD 511

Query: 549 LLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDL 598
           +LLAASEALASQVT+++F  GLI+PPFA IRKISA+IAASV AK YELGLA+ LP+P+DL
Sbjct: 512 MLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDL 571

BLAST of Csor.00g034920 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 828.2 bits (2138), Expect = 4.3e-240
Identity = 406/557 (72.89%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 69  ASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKK 128
           ++T +Q++TPW  SVASGY L+R+PR+NK LAFT+KERDAHYL GLLPP +L+Q+VQ +K
Sbjct: 32  SATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERK 91

Query: 129 MLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIF 188
           ++HN+R+Y VPL +YMA+MDLQERNE++FYKLLID+VEELLPVVYTPTVGEACQKYGSIF
Sbjct: 92  VMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIF 151

Query: 189 RHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 248
           R PQGLYISL EKGKILEVL+NWP+R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 152 RKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 211

Query: 249 TALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYG 308
           TALGG+ PS CLP+TIDVGTNNE+LLNDEFYIGL+Q+RATGQEYAE + EFM AVKQ+YG
Sbjct: 212 TALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYG 271

Query: 309 EKLL----------------------------IQGTASVVLAGLITALKFVGGSLADHRF 368
           EK+L                            IQGTASVVLAGLI A K +G  LADH F
Sbjct: 272 EKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLADHTF 331

Query: 369 LFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWA 428
           LFLGAGEAGTGIAELIAL+ISK+T AP+ ETRKK+WLVDSKGLIVSSRK++LQHFK+PWA
Sbjct: 332 LFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWA 391

Query: 429 HEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSEC 488
           HEHKP+K+LI AV  IKPTVLIGTSGVG+ FTKEVVE M + NEKP+I +LSNPTSQ+EC
Sbjct: 392 HEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAEC 451

Query: 489 TAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDD 548
           TAE+AY W++G AIF SGSPFDPV Y+GK ++PGQ+NN YIFPG GLGLIMSG IRV DD
Sbjct: 452 TAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDD 511

Query: 549 LLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDL 598
           +LLAASEALA+QVT++ +  GLI+PPF+ IR+ISA+IAA VAAK Y+LGLA+ LP+ +DL
Sbjct: 512 MLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDL 571

BLAST of Csor.00g034920 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 823.9 bits (2127), Expect = 8.1e-239
Identity = 404/557 (72.53%), Postives = 483/557 (86.71%), Query Frame = 0

Query: 69  ASTEEQIMTPWTVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKK 128
           ++T E  +TPW++SV+SGY+LLR+PR+NK LAFTEKERD HYLRGLLPP VL Q +Q K+
Sbjct: 25  SATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKR 84

Query: 129 MLHNIRRYQVPLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIF 188
           +L+NIR+YQ PL KYMA+ +LQERNE++FYKLLID+VEELLP+VYTPTVGEACQK+GSIF
Sbjct: 85  LLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIF 144

Query: 189 RHPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 248
           R PQGL+ISL++KGKIL+VL+NWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKL+LY
Sbjct: 145 RRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALY 204

Query: 249 TALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQHYG 308
           +ALGGV PS CLPVTIDVGTNNE+LLNDEFYIGLRQKRATGQEY+EL++EFM+AVKQ+YG
Sbjct: 205 SALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYG 264

Query: 309 EKLL----------------------------IQGTASVVLAGLITALKFVGGSLADHRF 368
           EK+L                            IQGTASVVLAGL++A K     LA+H F
Sbjct: 265 EKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAEHTF 324

Query: 369 LFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWA 428
           LFLGAGEAGTGIAELIAL +SKQ NA +EE+RKK+WLVDSKGLIV+SRKD+LQ FKKPWA
Sbjct: 325 LFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWA 384

Query: 429 HEHKPIKELIDAVKEIKPTVLIGTSGVGRAFTKEVVETMTSINEKPIIFSLSNPTSQSEC 488
           HEH+P+K+L+ A+K IKPTVLIG+SGVGR+FTKEV+E M+SINE+P+I +LSNPT+QSEC
Sbjct: 385 HEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLIMALSNPTTQSEC 444

Query: 489 TAEEAYKWSQGSAIFASGSPFDPVNYEGKVFVPGQSNNAYIFPGFGLGLIMSGTIRVHDD 548
           TAEEAY WS+G AIFASGSPFDPV YEGKVFV  Q+NNAYIFPGFGLGL++SG IRVHDD
Sbjct: 445 TAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLGLVISGAIRVHDD 504

Query: 549 LLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYELGLATRLPQPRDL 598
           +LLAA+EALA QV+++++++G+I+P F+ IRKISA IAA+VA KAYELGLA RLP+P+D+
Sbjct: 505 MLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAGRLPRPKDI 564

BLAST of Csor.00g034920 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 386.0 bits (990), Expect = 5.7e-107
Identity = 227/576 (39.41%), Postives = 334/576 (57.99%), Query Frame = 0

Query: 80  TVSVASGYNLLRNPRHNKELAFTEKERDAHYLRGLLPPTVLAQNVQVKKMLHNIRRYQV- 139
           T+    G ++L +P  NK  AFT  ER+   LRGLLPP V+    Q+ + + +++R +  
Sbjct: 40  TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99

Query: 140 ---------PLLKYMAMMDLQERNEKMFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFR 199
                     L K+  +  L +RNE M+YK+LI+++EE  P+VYTPTVG  CQ Y  +FR
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159

Query: 200 HPQGLYISLREKGKILEVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 259
            P+G+Y S  ++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI VGKL LY 
Sbjct: 160 RPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYV 219

Query: 260 ALGGVHPSVCLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAV------ 319
           A  G++P   LPV IDVGTNNE+L ND  Y+GL+Q+R    +Y ++IDEFM AV      
Sbjct: 220 AAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWPH 279

Query: 320 --------KQHYGEKLL-------------IQGTASVVLAGLITALKFVGGSLADH---R 379
                   +  +  KLL             +QGTA V +AGL+ A++  G  + D    +
Sbjct: 280 VIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMK 339

Query: 380 FLFLGAGEAGTGIAELIALEISK---QTNAPLEETRKKVWLVDSKGLIVSSRKD---TLQ 439
            +  GAG AG G+       +++    T    +  + + W+VD++GLI   R++     Q
Sbjct: 340 IVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQ 399

Query: 440 HF-KKPWAHEHKPIKE---LIDAVKEIKPTVLIGTSGVGRAFTKEVVETMT-SINEKPII 499
            F +K    E + +KE   L++ V+E+KP VL+G S VG  F+KEV+E M  S + +P I
Sbjct: 400 PFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAI 459

Query: 500 FSLSNPTSQSECTAEEAYKWSQGSAIFASGSPFDPVNY-EGKVFVPGQSNNAYIFPGFGL 559
           F++SNPT  +ECT ++A+     + IFASGSPF  V +  G V    Q NN Y+FPG GL
Sbjct: 460 FAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGL 519

Query: 560 GLIMSGTIRVHDDLLLAASEALASQVTQDDFDRGLIFPPFAKIRKISAHIAASVAAKAYE 594
           G ++SG   V D +L AASE LA+ +++++   G+I+PP ++IR I+  IAA+V  +A E
Sbjct: 520 GTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIE 579

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P364442.1e-25269.70NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ... [more]
P516151.2e-25072.88NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9CA832.6e-25069.68NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P432791.3e-24975.72NADP-dependent malic enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
P126282.1e-24775.40NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6581878.10.0100.00NADP-dependent malic enzyme, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
KAG7018314.10.095.36NADP-dependent malic enzyme, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_022956197.10.095.04NADP-dependent malic enzyme-like [Cucurbita moschata][more]
XP_022979251.10.094.24NADP-dependent malic enzyme-like [Cucurbita maxima][more]
XP_023527958.10.093.92NADP-dependent malic enzyme-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GVN60.095.04Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111457964 PE=3 SV=1[more]
A0A6J1IQ940.094.24Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479032 PE=3 SV=1[more]
A0A6J1DJF80.084.86Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1[more]
A0A1S4DWR50.084.25Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1[more]
A0A5D3CAD90.084.25Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 P... [more]
Match NameE-valueIdentityDescription
AT1G79750.11.9e-25169.68NADP-malic enzyme 4 [more]
AT5G25880.11.4e-24373.61NADP-malic enzyme 3 [more]
AT5G11670.14.3e-24072.89NADP-malic enzyme 2 [more]
AT2G19900.18.1e-23972.53NADP-malic enzyme 1 [more]
AT2G13560.15.7e-10739.41NAD-dependent malic enzyme 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 216..245
score: 82.75
coord: 252..274
score: 67.93
coord: 156..180
score: 65.6
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 312..597
e-value: 7.0E-111
score: 369.5
coord: 58..314
e-value: 5.5E-102
score: 340.1
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 313..566
e-value: 8.7E-110
score: 380.7
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 313..565
e-value: 8.8E-95
score: 316.8
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 150..325
e-value: 7.3E-87
score: 304.6
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 150..314
e-value: 2.4E-70
score: 236.1
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 84..315
e-value: 9.4E-111
score: 371.0
NoneNo IPR availableGENE3D3.40.50.720coord: 316..597
e-value: 8.3E-118
score: 395.1
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 313..597
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 313..597
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 14..314
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 14..314
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 313..591
e-value: 2.81466E-143
score: 414.253
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 70..314
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 313..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g034920.m01Csor.00g034920.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity