Csor.00g013770 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g013770
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionsubtilisin-like protease SBT1.2
LocationCsor_Chr02: 10038833 .. 10041145 (+)
RNA-Seq ExpressionCsor.00g013770
SyntenyCsor.00g013770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCAACTCCCAAATGCTTCTTCTTTCAACCCTTTTCCTCTGTTTTCTTTCACTTCAAGTTCAAGCAAACATTCCCACTCTTCAAACTTACATAATTCAACTCCATCCACAGGGATTAACCAGCTCTCCCTTTTATTCTAAGCGTCAATGGCATCTTTCTTTTCTTGAACAAACTCTATCTGCTGAAGAAGACTCTTCTTCTCGATTGCTTTACTCGTATTCTAATGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGATTGAGTATTTGAAGAAATTGCCTGATGTTGTGGCTGTGAGAGCTGATAGAAAATATGAAATTCAAACTACTTATTCTCCTAAGTTCTTGGGGCTTAGTTTAGGCTCACAAGGTGTTTGGTTTAATTCCTCAATGGGCGAAGGAGCGATTGTTGGGGTTCTTGACACTGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAGAATGCCCCCAATTCCTCAAAAATGGCGAGGGGCTTGCCAAGAAGGCCAAGATTTTAACTCCTCAAATTGTAACAGGAAACTCATTGGTGCCAAGTTCTTTATCAAAGGACACCATGTGGCTTCATCCCAGCCATCTGATGTTGATCAGGAGTATGTCTCCCCAAGAGACTCCCATGGCCATGGAACTCACACGTCTTCTACAGCTGCAGGAGCTTCAGTCGCTGAAGCGAGCGTGTTCGGTAATGGAGCTGGCGTGGCGCAAGGGATGGCCCCAAGAGCTCACATTGCGGTGTACAAAGTTTGCTGGTTCAGTGGCTGCTATAGCTCTGATATTGTAGCAGCCATGGATGCTGCAATAAGGGATGGTGTTGACATTCTCTCCCTTTCGCTCGGTGGCTTCCCGCTTCCATTTTTCGACGACAGTATCGCCATCGGAAGCTTTCGAGCAATGCAGCATGGCATCTCAGTCAACTGCGCAGCAGGAAACAATGGTCCAATTCAAAGCTCTGTTGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAAGCACACTCGACCGAAGATTCCCGGCTATCGTTCGACTAAGCAATGGGGAATTCATTTACGGCGAATCAATGTACCCTGGCAACAAGTTCAAGCATGCTACCAAAGAGCTTGAAGTGGTTTACTTGACTGGAGGACAAATGGGAGGTGAACTTTGCTTAAAAGGGTCTCTTCCAAGAGAACAAGTAGAAGGAAAAATCGTGCTCTGCGACCGTGGCGTCAATGGCAGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCCATGATCCTTGCAAATACAGCGATAAATCTAGAGGAAGACTTGGTTGATGTTCATGTTTTACCGGCCACATTGATCGGATTTGCCGAGGCGAATCGGTTAAAAGCTTACATGAACACAACAAGCAATCCAAAAGCCAGAATCCAATTTGGAGGAACTGTGATAGGAAGATCAAGAGCTCCATCTGTTGCTCAATTTTCATCAAGAGGACCAAGCCTCACAAATCCTTCAACGCTCAAGCCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCACAAAATCTTGGTCCAACTGGCCTTCCAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGGCTTGTCCCCATGTCAGTGGAATTGCGGCTCTAATCCATTCAGCTCATCCAAAATGGACACCAGCAGCCATTAAATCAGCCATAATGACAACCGCTGAAGTTACTGATCATTATGGTAAGCCAATTCTTGATGGCAGCAAACCAGCTGGCGTTTTTGCAATGGGAGCTGGTCATATAAACCCAACCAAAGCAATTGATCCTGGTTTAGTTTATGATATAAAGCCATATGAATATGTCATTCATCTTTGTGCTCTTGGATACACTCATTCAGAAATCTTCATCATAACCCACATGAATGTGAGTTGCCATAAGGTTTTGCAGATGAACAAAGGCTTCAGCTTGAACTATCCTTCCATGTCCGTCGTTTTTAAGCACAAGACGACGAGTAAGGCAGTTTCGAGACGATTGACGAATGTGGGGAGCCCAAATTCCATCTACTCAGTGGAAGTAACGGCACCTAAAGGCGTTCAAGTTCGAGTCAAGCCTCGGCGCTTGGTGTTCAAATATGTTAACCAAAGTTTAAATTATAAGGTATGGTTTATGTCTGAGAAGGGAAAAGAAGGAAAAACAGTGAGCTCTTCAGAAGGGCATTTGACATGGGTTCATTCGGAGAATGGGAAATACAAAGTTAGAAGCCCAATTGTGGTGACTTGGAAGAATTAA

mRNA sequence

ATGGATTTCAACTCCCAAATGCTTCTTCTTTCAACCCTTTTCCTCTGTTTTCTTTCACTTCAAGTTCAAGCAAACATTCCCACTCTTCAAACTTACATAATTCAACTCCATCCACAGGGATTAACCAGCTCTCCCTTTTATTCTAAGCGTCAATGGCATCTTTCTTTTCTTGAACAAACTCTATCTGCTGAAGAAGACTCTTCTTCTCGATTGCTTTACTCGTATTCTAATGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGATTGAGTATTTGAAGAAATTGCCTGATGTTGTGGCTGTGAGAGCTGATAGAAAATATGAAATTCAAACTACTTATTCTCCTAAGTTCTTGGGGCTTAGTTTAGGCTCACAAGGTGTTTGGTTTAATTCCTCAATGGGCGAAGGAGCGATTGTTGGGGTTCTTGACACTGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAGAATGCCCCCAATTCCTCAAAAATGGCGAGGGGCTTGCCAAGAAGGCCAAGATTTTAACTCCTCAAATTGTAACAGGAAACTCATTGGTGCCAAGTTCTTTATCAAAGGACACCATGTGGCTTCATCCCAGCCATCTGATGTTGATCAGGAGTATGTCTCCCCAAGAGACTCCCATGGCCATGGAACTCACACGTCTTCTACAGCTGCAGGAGCTTCAGTCGCTGAAGCGAGCGTGTTCGGTAATGGAGCTGGCGTGGCGCAAGGGATGGCCCCAAGAGCTCACATTGCGGTGTACAAAGTTTGCTGGTTCAGTGGCTGCTATAGCTCTGATATTGTAGCAGCCATGGATGCTGCAATAAGGGATGGTGTTGACATTCTCTCCCTTTCGCTCGGTGGCTTCCCGCTTCCATTTTTCGACGACAGTATCGCCATCGGAAGCTTTCGAGCAATGCAGCATGGCATCTCAGTCAACTGCGCAGCAGGAAACAATGGTCCAATTCAAAGCTCTGTTGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAAGCACACTCGACCGAAGATTCCCGGCTATCGTTCGACTAAGCAATGGGGAATTCATTTACGGCGAATCAATGTACCCTGGCAACAAGTTCAAGCATGCTACCAAAGAGCTTGAAGTGGTTTACTTGACTGGAGGACAAATGGGAGGTGAACTTTGCTTAAAAGGGTCTCTTCCAAGAGAACAAGTAGAAGGAAAAATCGTGCTCTGCGACCGTGGCGTCAATGGCAGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCCATGATCCTTGCAAATACAGCGATAAATCTAGAGGAAGACTTGGTTGATGTTCATGTTTTACCGGCCACATTGATCGGATTTGCCGAGGCGAATCGGTTAAAAGCTTACATGAACACAACAAGCAATCCAAAAGCCAGAATCCAATTTGGAGGAACTGTGATAGGAAGATCAAGAGCTCCATCTGTTGCTCAATTTTCATCAAGAGGACCAAGCCTCACAAATCCTTCAACGCTCAAGCCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCACAAAATCTTGGTCCAACTGGCCTTCCAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGGCTTGTCCCCATGTCAGTGGAATTGCGGCTCTAATCCATTCAGCTCATCCAAAATGGACACCAGCAGCCATTAAATCAGCCATAATGACAACCGCTGAAGTTACTGATCATTATGGTAAGCCAATTCTTGATGGCAGCAAACCAGCTGGCGTTTTTGCAATGGGAGCTGGTCATATAAACCCAACCAAAGCAATTGATCCTGGTTTAGTTTATGATATAAAGCCATATGAATATGTCATTCATCTTTGTGCTCTTGGATACACTCATTCAGAAATCTTCATCATAACCCACATGAATGTGAGTTGCCATAAGGTTTTGCAGATGAACAAAGGCTTCAGCTTGAACTATCCTTCCATGTCCGTCGTTTTTAAGCACAAGACGACGAGTAAGGCAGTTTCGAGACGATTGACGAATGTGGGGAGCCCAAATTCCATCTACTCAGTGGAAGTAACGGCACCTAAAGGCGTTCAAGTTCGAGTCAAGCCTCGGCGCTTGGTGTTCAAATATGTTAACCAAAGTTTAAATTATAAGGTATGGTTTATGTCTGAGAAGGGAAAAGAAGGAAAAACAGTGAGCTCTTCAGAAGGGCATTTGACATGGGTTCATTCGGAGAATGGGAAATACAAAGTTAGAAGCCCAATTGTGGTGACTTGGAAGAATTAA

Coding sequence (CDS)

ATGGATTTCAACTCCCAAATGCTTCTTCTTTCAACCCTTTTCCTCTGTTTTCTTTCACTTCAAGTTCAAGCAAACATTCCCACTCTTCAAACTTACATAATTCAACTCCATCCACAGGGATTAACCAGCTCTCCCTTTTATTCTAAGCGTCAATGGCATCTTTCTTTTCTTGAACAAACTCTATCTGCTGAAGAAGACTCTTCTTCTCGATTGCTTTACTCGTATTCTAATGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGATTGAGTATTTGAAGAAATTGCCTGATGTTGTGGCTGTGAGAGCTGATAGAAAATATGAAATTCAAACTACTTATTCTCCTAAGTTCTTGGGGCTTAGTTTAGGCTCACAAGGTGTTTGGTTTAATTCCTCAATGGGCGAAGGAGCGATTGTTGGGGTTCTTGACACTGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAGAATGCCCCCAATTCCTCAAAAATGGCGAGGGGCTTGCCAAGAAGGCCAAGATTTTAACTCCTCAAATTGTAACAGGAAACTCATTGGTGCCAAGTTCTTTATCAAAGGACACCATGTGGCTTCATCCCAGCCATCTGATGTTGATCAGGAGTATGTCTCCCCAAGAGACTCCCATGGCCATGGAACTCACACGTCTTCTACAGCTGCAGGAGCTTCAGTCGCTGAAGCGAGCGTGTTCGGTAATGGAGCTGGCGTGGCGCAAGGGATGGCCCCAAGAGCTCACATTGCGGTGTACAAAGTTTGCTGGTTCAGTGGCTGCTATAGCTCTGATATTGTAGCAGCCATGGATGCTGCAATAAGGGATGGTGTTGACATTCTCTCCCTTTCGCTCGGTGGCTTCCCGCTTCCATTTTTCGACGACAGTATCGCCATCGGAAGCTTTCGAGCAATGCAGCATGGCATCTCAGTCAACTGCGCAGCAGGAAACAATGGTCCAATTCAAAGCTCTGTTGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAAGCACACTCGACCGAAGATTCCCGGCTATCGTTCGACTAAGCAATGGGGAATTCATTTACGGCGAATCAATGTACCCTGGCAACAAGTTCAAGCATGCTACCAAAGAGCTTGAAGTGGTTTACTTGACTGGAGGACAAATGGGAGGTGAACTTTGCTTAAAAGGGTCTCTTCCAAGAGAACAAGTAGAAGGAAAAATCGTGCTCTGCGACCGTGGCGTCAATGGCAGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCCATGATCCTTGCAAATACAGCGATAAATCTAGAGGAAGACTTGGTTGATGTTCATGTTTTACCGGCCACATTGATCGGATTTGCCGAGGCGAATCGGTTAAAAGCTTACATGAACACAACAAGCAATCCAAAAGCCAGAATCCAATTTGGAGGAACTGTGATAGGAAGATCAAGAGCTCCATCTGTTGCTCAATTTTCATCAAGAGGACCAAGCCTCACAAATCCTTCAACGCTCAAGCCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCACAAAATCTTGGTCCAACTGGCCTTCCAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGGCTTGTCCCCATGTCAGTGGAATTGCGGCTCTAATCCATTCAGCTCATCCAAAATGGACACCAGCAGCCATTAAATCAGCCATAATGACAACCGCTGAAGTTACTGATCATTATGGTAAGCCAATTCTTGATGGCAGCAAACCAGCTGGCGTTTTTGCAATGGGAGCTGGTCATATAAACCCAACCAAAGCAATTGATCCTGGTTTAGTTTATGATATAAAGCCATATGAATATGTCATTCATCTTTGTGCTCTTGGATACACTCATTCAGAAATCTTCATCATAACCCACATGAATGTGAGTTGCCATAAGGTTTTGCAGATGAACAAAGGCTTCAGCTTGAACTATCCTTCCATGTCCGTCGTTTTTAAGCACAAGACGACGAGTAAGGCAGTTTCGAGACGATTGACGAATGTGGGGAGCCCAAATTCCATCTACTCAGTGGAAGTAACGGCACCTAAAGGCGTTCAAGTTCGAGTCAAGCCTCGGCGCTTGGTGTTCAAATATGTTAACCAAAGTTTAAATTATAAGGTATGGTTTATGTCTGAGAAGGGAAAAGAAGGAAAAACAGTGAGCTCTTCAGAAGGGCATTTGACATGGGTTCATTCGGAGAATGGGAAATACAAAGTTAGAAGCCCAATTGTGGTGACTTGGAAGAATTAA

Protein sequence

MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTLSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLGLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSNCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIYGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEKGQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQFGGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAGVFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYVNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Homology
BLAST of Csor.00g013770 vs. ExPASy Swiss-Prot
Match: O64495 (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 1.1e-301
Identity = 508/767 (66.23%), Postives = 627/767 (81.75%), Query Frame = 0

Query: 14  FLCFLSL---QVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTL----SAEED 73
           FLC + L      + I   QTYI+QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 74  SSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL-SLG 133
            SSRLLYSY +A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS KFLGL   G
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 134 SQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSNCNRK 193
           + GVW  S  G+G I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS+CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 194 LIGAKFFIKGHHVASS--QPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGAG 253
           LIGA+FFI+GH VA+S  +  ++ +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 254 VAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAI 313
           VA+GMAP AHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 314 GSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIYGE 373
           G+FRAM+ GISV CAAGNNGPI+SSVAN APW++TIGA TLDRRFPA+VRL+NG+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 374 SMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEKGQI 433
           S+YPG   K+A +E+EV+Y+TGG  G E CL+GSLPRE++ GK+V+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 434 VKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQFGGT 493
           VKE+GG AMILANT IN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 494 VIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFT 553
           VIGRSRAP VAQFS+RGPSL NPS LKPD+IAPGVNIIAAWPQNLGPTGLP DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 554 VMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAGVFA 613
           VMSGTSM+CPHVSGI ALI SA+P W+PAAIKSA+MTTA++ D  GK I DG+KPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 614 MGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFS 673
           +GAGH+NP KAI+PGLVY+I+P +Y+ +LC LG+T S+I  ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 674 LNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYVNQS 733
           LNYPS++V+FK   T++ ++RR+TNVGSPNSIYSV V AP+G++V V P+RLVFK+V+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 734 LNYKVWF-MSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWK 770
           L+Y+VWF + +K + GK  S ++G LTWV+S N   +VRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Csor.00g013770 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 5.1e-190
Identity = 367/769 (47.72%), Postives = 491/769 (63.85%), Query Frame = 0

Query: 4   NSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTLSA 63
           +S  + + T FL FL L   A     +TYII+++      S F +   W+ S L      
Sbjct: 7   SSSSITIITTFL-FLLLHTTAK----KTYIIRVNHSDKPES-FLTHHDWYTSQL------ 66

Query: 64  EEDSSSRLLYSYSNAMEGFAAQLSETEIE-YLKKLPDVVAVRADRKYEIQTTYSPKFLGL 123
             +S S LLY+Y+ +  GF+A L  TE +  L     ++ +  D  Y + TT +P+FLGL
Sbjct: 67  --NSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 126

Query: 124 SLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSNC 183
           +    GV    S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S  C
Sbjct: 127 N-SEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 186

Query: 184 NRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGA 243
           N+KLIGA+ F KG  +AS       +E VSPRD  GHGTHTS+TAAG++V  AS  G  A
Sbjct: 187 NKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAA 246

Query: 244 GVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 303
           G A+GMA RA +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IA
Sbjct: 247 GTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIA 306

Query: 304 IGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIYG 363
           IG+F AM+ G+ V+C+AGN+GP ++SVANVAPW+ T+GA TLDR FPA   L NG+ + G
Sbjct: 307 IGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTG 366

Query: 364 ESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEKGQ 423
            S+Y G      TK LE+VY  G      LCL GSL    V GKIV+CDRGVN R EKG 
Sbjct: 367 VSLYSG--VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 426

Query: 424 IVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQFGG 483
           +V+++GG  MI+ANTA + EE + D H+LPA  +G    + L+ Y+ + S P A + F G
Sbjct: 427 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 486

Query: 484 TVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNF 543
           TV+    +P VA FSSRGP+   P  LKPDVI PGVNI+A W   +GPTGL +DSRR+ F
Sbjct: 487 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 546

Query: 544 TVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDG--SKPAG 603
            +MSGTSM+CPH+SG+A L+ +AHP+W+P+AIKSA+MTTA V D+   P+ D   +  + 
Sbjct: 547 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 606

Query: 604 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHKVLQMN 663
            +A G+GH++P KA+ PGLVYDI   EY+  LC+L YT   I  I+   +V+C K  + +
Sbjct: 607 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFS 666

Query: 664 KGFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKY 723
               LNYPS SV+F  K   +  +R +TNVG+ +S+Y V V     V + VKP +L FK 
Sbjct: 667 DPGQLNYPSFSVLFGGKRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 726

Query: 724 VNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
           V +   Y V F+S+KG    T  +  G +TW    N +++VRSP+  +W
Sbjct: 727 VGEKKRYTVTFVSKKG-VSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Csor.00g013770 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.8e-182
Identity = 360/775 (46.45%), Postives = 489/775 (63.10%), Query Frame = 0

Query: 5   SQMLLLSTLF---LCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTL 64
           S   L ST F   LC     V ++     TYI+ +    + SS F     W+ S L    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI- 61

Query: 65  SAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLG 124
                 S+ LLY+Y NA+ GF+ +L++ E + L   P V++V  + +YE+ TT +P FLG
Sbjct: 62  ----SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 121

Query: 125 LSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSN 184
           L   +  ++  +      +VGVLDTGVWPES S+SD    PIP  W+G C+ G +F +S 
Sbjct: 122 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 181

Query: 185 CNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNG 244
           CNRKLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  
Sbjct: 182 CNRKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 241

Query: 245 AGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSI 304
           +G A+GMAPRA +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +
Sbjct: 242 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 301

Query: 305 AIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIY 364
           AIG+F AM+ GI V+C+AGN GP  SS++NVAPWITT+GA TLDR FPA+  L NG+   
Sbjct: 302 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 361

Query: 365 GESMYPGNKFKHATKELEVVYL--TGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSE 424
           G S++ G       K L  +Y         G LC+ G+L  E+V+GKIV+CDRG+N R +
Sbjct: 362 GVSLFKGEAL--PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 421

Query: 425 KGQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQ 484
           KG +VK +GG  MILANTA N EE + D H+LPAT +G    + ++ Y+ T  NP A I 
Sbjct: 422 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 481

Query: 485 FGGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRR 544
             GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPTGL  DSRR
Sbjct: 482 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 541

Query: 545 SNFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILD--GSK 604
             F ++SGTSM+CPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T   GKP+LD    K
Sbjct: 542 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 601

Query: 605 PAGVFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQ 664
           P+  F  GAGH++PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C     
Sbjct: 602 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC----D 661

Query: 665 MNKGFS---LNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTA-PKGVQVRVKPR 724
            +K +S   LNYPS +V        K  +R +T+VG   + YSV+VT+   GV++ V+P 
Sbjct: 662 PSKSYSVADLNYPSFAVNVDGVGAYK-YTRTVTSVGGAGT-YSVKVTSETTGVKISVEPA 721

Query: 725 RLVFKYVNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
            L FK  N+  +Y V F  +  K   + S+S G + W    +GK+ V SP+ ++W
Sbjct: 722 VLNFKEANEKKSYTVTFTVDSSK--PSGSNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Csor.00g013770 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 3.3e-181
Identity = 347/775 (44.77%), Postives = 483/775 (62.32%), Query Frame = 0

Query: 7   MLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFL----EQTLS 66
           + ++ ++ L FL  +    I T +TY+I +    +   P+ +  QW+ S +    +    
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAM-PLPYTNHLQWYSSKINSVTQHKSQ 71

Query: 67  AEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL 126
            EE +++R+LY+Y  A  G AAQL++ E E L++   VVAV  + +YE+ TT SP FLGL
Sbjct: 72  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 131

Query: 127 S-LGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSN 186
               S+ VW         +VGVLDTG+WPES SF+D+ M P+P  WRGAC+ G+ F   N
Sbjct: 132 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 191

Query: 187 CNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNG 246
           CNRK++GA+ F +G+  A+ +  D + EY SPRD  GHGTHT++T AG+ V  A++FG  
Sbjct: 192 CNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 251

Query: 247 AGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSI 306
            G A+GMA +A +A YKVCW  GC+SSDI++A+D A+ DGV +LS+SLGG    +  DS+
Sbjct: 252 YGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSL 311

Query: 307 AIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIY 366
           +I +F AM+ G+ V+C+AGN GP   S+ NV+PWITT+GAST+DR FPA V++       
Sbjct: 312 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 371

Query: 367 GESMYPGNKFKHATKELEVVYLTGGQMGGE---LCLKGSLPREQVEGKIVLCDRGVNGRS 426
           G S+Y G       K+  +VYL       +    CL G+L R  V GKIV+CDRGV  R 
Sbjct: 372 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 431

Query: 427 EKGQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARI 486
           +KGQ+VK +GG  M+L NTA N EE + D H+LPA  +G  E   +K Y  T+    A +
Sbjct: 432 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 491

Query: 487 QFGGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSR 546
           +  GT IG   +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++ P+ L  D R
Sbjct: 492 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 551

Query: 547 RSNFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILD--GS 606
           R  F ++SGTSM+CPHVSG+AALI S HP W+PAAIKSA+MTTA V D+  KP+ D  G+
Sbjct: 552 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 611

Query: 607 KPAGVFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIIT-HMNVSCHKV 666
            P+  +  GAGHI+P +A DPGLVYDI P EY   LC    + S++ + T H N +C   
Sbjct: 612 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 671

Query: 667 LQMNKGFSLNYPSMSVVFKHKTTSKAVS--RRLTNVGSPNSIYSVEVTAPKGVQVRVKPR 726
           L  N G +LNYP++S +F   T  KA++  R +TNVG   S Y V V+  KG  V V+P+
Sbjct: 672 LAKNPG-NLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 731

Query: 727 RLVFKYVNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
            L F   +Q L+Y V F +      +      G L W   ++  +KVRSP+++TW
Sbjct: 732 TLNFTSKHQKLSYTVTFRT----RFRMKRPEFGGLVW---KSTTHKVRSPVIITW 776

BLAST of Csor.00g013770 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 4.8e-180
Identity = 341/755 (45.17%), Postives = 480/755 (63.58%), Query Frame = 0

Query: 31  TYIIQLHPQGLTSSPFYSKRQWHLSFLEQTLSAEEDSSSRLLYSYSNAMEGFAAQLSETE 90
           TYI+ +  +    S F +   W+ S    +L++   S   ++++Y     GF+A+L+  +
Sbjct: 27  TYIVHVDHEA-KPSIFPTHFHWYTS----SLASLTSSPPSIIHTYDTVFHGFSARLTSQD 86

Query: 91  IEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL-SLGSQGVWFNSSMGEGAIVGVLDTGVW 150
              L   P V++V  ++   + TT SP+FLGL S    G+   S  G   ++GV+DTGVW
Sbjct: 87  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 146

Query: 151 PESPSFSDSRMPPIPQKWRGACQEGQDFNSSNCNRKLIGAKFFIKGHHVASSQPSDVDQE 210
           PE PSF D  + P+P KW+G C   QDF  S CNRKL+GA+FF  G+   + + ++   E
Sbjct: 147 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNET-TE 206

Query: 211 YVSPRDSHGHGTHTSSTAAGASVAEASVFGNGAGVAQGMAPRAHIAVYKVCWFSGCYSSD 270
           + SPRDS GHGTHT+S +AG  V  AS  G   GVA GMAP+A +A YKVCW SGCY SD
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSD 266

Query: 271 IVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVNCAAGNNGPIQSSV 330
           I+AA D A+ DGVD++SLS+GG  +P++ D+IAIG+F A+  GI V+ +AGN GP   +V
Sbjct: 267 ILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTV 326

Query: 331 ANVAPWITTIGASTLDRRFPAIVRLSNGEFIYGESMYPGNKFKHATKELEVVYLTGGQMG 390
            NVAPW+TT+GA T+DR FPA V+L NG+ I G S+Y G       +   +VY  G  +G
Sbjct: 327 TNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPG-RMYPLVY-GGSLLG 386

Query: 391 GE-----LCLKGSLPREQVEGKIVLCDRGVNGRSEKGQIVKESGGAAMILANTAINLEED 450
           G+     LCL+GSL    V+GKIVLCDRG+N R+ KG+IV+++GG  MI+AN   + E  
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 451 LVDVHVLPATLIGFAEANRLKAYMNTTS------NPKARIQFGGTVIGRSRAPSVAQFSS 510
           + D HVLPAT +G +  + ++ Y++ +S      +P A I F GT +G   AP VA FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 511 RGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTVMSGTSMACPHVSGI 570
           RGP+   P  LKPDVIAPG+NI+AAWP  +GP+G+  D+RR+ F ++SGTSMACPHVSG+
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 571 AALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGS--KPAGVFAMGAGHINPTKAID 630
           AAL+ +AHP W+PAAI+SA++TTA   D+ G+P++D S    + V   G+GH++PTKA+D
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 631 PGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFSLNYPSMSVVFKHK 690
           PGLVYDI  Y+Y+  LC   YT + I  IT     C    +     +LNYPS SVVF+  
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 691 TTSKAVS---RRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYVNQSLNYKVWFMSE 750
             SK  +   R +TNVG  +S+Y +++  P+G  V V+P +L F+ V Q L++ V   + 
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 751 KGKEGKTVSSSE-GHLTWVHSENGKYKVRSPIVVT 768
           + K     ++ E GH+ W    +GK  V SP+VVT
Sbjct: 747 EVKLSPGATNVETGHIVW---SDGKRNVTSPLVVT 770

BLAST of Csor.00g013770 vs. NCBI nr
Match: KAG6606345.1 (Subtilisin-like protease 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1540 bits (3986), Expect = 0.0
Identity = 770/770 (100.00%), Postives = 770/770 (100.00%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. NCBI nr
Match: KAG7036287.1 (Subtilisin-like protease SBT1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1537 bits (3980), Expect = 0.0
Identity = 769/770 (99.87%), Postives = 769/770 (99.87%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKT SKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTMSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. NCBI nr
Match: XP_022930926.1 (subtilisin-like protease SBT1.2 [Cucurbita moschata])

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 765/770 (99.35%), Postives = 768/770 (99.74%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLG+QGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMP IPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCN+KLIGAKFFIKGHHVASS PSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEAN+LKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANQLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. NCBI nr
Match: XP_022995265.1 (subtilisin-like protease SBT1.2 [Cucurbita maxima])

HSP 1 Score: 1519 bits (3933), Expect = 0.0
Identity = 759/770 (98.57%), Postives = 764/770 (99.22%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQAN  TLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRAD+KYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLGSQGVWFNS+MGEGAIVGVLDTGVWPESPSFSDSRMP IPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCN+KLIGAKFFIKGHHVASS PSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANT INLEEDLVDVHVLPATLIGF+EANRLKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ VSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. NCBI nr
Match: XP_023532819.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1517 bits (3927), Expect = 0.0
Identity = 756/770 (98.18%), Postives = 764/770 (99.22%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQA+  TLQTY+IQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQADTSTLQTYLIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYLKKLPDVVAVRADRKYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLG+QGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCN+KLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRL NGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLRNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANT INLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWT AAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTAAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKA+SRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAISRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ VSSSEGHLTWVHSENGKYKVRSP+VVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPMVVTWKN 770

BLAST of Csor.00g013770 vs. ExPASy TrEMBL
Match: A0A6J1ES95 (subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111437268 PE=3 SV=1)

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 765/770 (99.35%), Postives = 768/770 (99.74%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLG+QGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMP IPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCN+KLIGAKFFIKGHHVASS PSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEAN+LKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANQLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. ExPASy TrEMBL
Match: A0A6J1K3M4 (subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111490865 PE=3 SV=1)

HSP 1 Score: 1519 bits (3933), Expect = 0.0
Identity = 759/770 (98.57%), Postives = 764/770 (99.22%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFNSQMLLLSTLFLCFLSLQVQAN  TLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRAD+KYEIQTTYSPKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLSLGSQGVWFNS+MGEGAIVGVLDTGVWPESPSFSDSRMP IPQKWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCN+KLIGAKFFIKGHHVASS PSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILANT INLEEDLVDVHVLPATLIGF+EANRLKAYMNTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ VSSSEGHLTWVHSENGKYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Csor.00g013770 vs. ExPASy TrEMBL
Match: A0A0A0LDY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=3 SV=1)

HSP 1 Score: 1389 bits (3596), Expect = 0.0
Identity = 692/770 (89.87%), Postives = 731/770 (94.94%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFN++M  L  LFLCFLSL VQ N  TLQTYIIQLHP GL +S F SK QWHLSFLEQ+
Sbjct: 1   MDFNARMGFL--LFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQS 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYLK+LPDVVAVR DRKY+IQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLS+G+QG+   SSMG+GAIVGVLDTGVWPESPSFSDS+MPP+PQKWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCNRKLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGASVA+ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGA TLDRRFPAI+RLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPRE+V+GK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILAN+ INLEEDLVDVHVLPATLIGFAEANRLKAY+NTTSNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGI ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GK ILDG+KPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHK+LQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GF+LNYPS+SV+FKH TTSK VSRRLTNVGS NSIY V+VTAP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFTLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ V  +EG LTW+H EN KYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Csor.00g013770 vs. ExPASy TrEMBL
Match: A0A5A7T534 (Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold154G00500 PE=3 SV=1)

HSP 1 Score: 1387 bits (3589), Expect = 0.0
Identity = 693/770 (90.00%), Postives = 728/770 (94.55%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFN++M  L  LFL FLSL VQ N  TLQTYIIQLHP GL +S F SK QWHLSFLEQT
Sbjct: 1   MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQL+ETE+EYLKKLPDVVAVR DRKY+IQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLS+G+QGVW  SSMG+GAIVG+LDTGVWPESPSFSDS+MPPIPQKWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCNRKLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGASVA+ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISV CAAGNNGP QSSVANVAPWITTIGA TLDRRFPAIVRLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPRE+V+GK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILAN+ INLEEDLVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGI ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GK ILDG+KPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GF+LNYPSMSV+FKH TTSK VSRRLTNVGS NSIY V+VTAP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ V  +EG LTW+H EN KYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Csor.00g013770 vs. ExPASy TrEMBL
Match: A0A1S3BHA2 (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=1)

HSP 1 Score: 1387 bits (3589), Expect = 0.0
Identity = 693/770 (90.00%), Postives = 728/770 (94.55%), Query Frame = 0

Query: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
           MDFN++M  L  LFL FLSL VQ N  TLQTYIIQLHP GL +S F SK QWHLSFLEQT
Sbjct: 1   MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
           LSAEEDSSSRLLYSYSNAMEGFAAQL+ETE+EYLKKLPDVVAVR DRKY+IQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
           GLS+G+QGVW  SSMG+GAIVG+LDTGVWPESPSFSDS+MPPIPQKWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180

Query: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
           NCNRKLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGASVA+ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
           IAIGSFRAMQHGISV CAAGNNGP QSSVANVAPWITTIGA TLDRRFPAIVRLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360

Query: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPRE+V+GK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
           GQIVKESGGAAMILAN+ INLEEDLVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
           NFTVMSGTSMACPHVSGI ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GK ILDG+KPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
           GF+LNYPSMSV+FKH TTSK VSRRLTNVGS NSIY V+VTAP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
           NQSLNYKVWFMSEKGKEG+ V  +EG LTW+H EN KYKVRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Csor.00g013770 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 1036.9 bits (2680), Expect = 7.9e-303
Identity = 508/767 (66.23%), Postives = 627/767 (81.75%), Query Frame = 0

Query: 14  FLCFLSL---QVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTL----SAEED 73
           FLC + L      + I   QTYI+QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 74  SSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL-SLG 133
            SSRLLYSY +A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS KFLGL   G
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 134 SQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSNCNRK 193
           + GVW  S  G+G I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS+CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 194 LIGAKFFIKGHHVASS--QPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGAG 253
           LIGA+FFI+GH VA+S  +  ++ +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 254 VAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAI 313
           VA+GMAP AHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 314 GSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIYGE 373
           G+FRAM+ GISV CAAGNNGPI+SSVAN APW++TIGA TLDRRFPA+VRL+NG+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 374 SMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEKGQI 433
           S+YPG   K+A +E+EV+Y+TGG  G E CL+GSLPRE++ GK+V+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 434 VKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQFGGT 493
           VKE+GG AMILANT IN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 494 VIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFT 553
           VIGRSRAP VAQFS+RGPSL NPS LKPD+IAPGVNIIAAWPQNLGPTGLP DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 554 VMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAGVFA 613
           VMSGTSM+CPHVSGI ALI SA+P W+PAAIKSA+MTTA++ D  GK I DG+KPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 614 MGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFS 673
           +GAGH+NP KAI+PGLVY+I+P +Y+ +LC LG+T S+I  ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 674 LNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYVNQS 733
           LNYPS++V+FK   T++ ++RR+TNVGSPNSIYSV V AP+G++V V P+RLVFK+V+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 734 LNYKVWF-MSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWK 770
           L+Y+VWF + +K + GK  S ++G LTWV+S N   +VRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Csor.00g013770 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 666.0 bits (1717), Expect = 3.7e-191
Identity = 367/769 (47.72%), Postives = 491/769 (63.85%), Query Frame = 0

Query: 4   NSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTLSA 63
           +S  + + T FL FL L   A     +TYII+++      S F +   W+ S L      
Sbjct: 7   SSSSITIITTFL-FLLLHTTAK----KTYIIRVNHSDKPES-FLTHHDWYTSQL------ 66

Query: 64  EEDSSSRLLYSYSNAMEGFAAQLSETEIE-YLKKLPDVVAVRADRKYEIQTTYSPKFLGL 123
             +S S LLY+Y+ +  GF+A L  TE +  L     ++ +  D  Y + TT +P+FLGL
Sbjct: 67  --NSESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL 126

Query: 124 SLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSNC 183
           +    GV    S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S  C
Sbjct: 127 N-SEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLC 186

Query: 184 NRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGA 243
           N+KLIGA+ F KG  +AS       +E VSPRD  GHGTHTS+TAAG++V  AS  G  A
Sbjct: 187 NKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAA 246

Query: 244 GVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 303
           G A+GMA RA +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IA
Sbjct: 247 GTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIA 306

Query: 304 IGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIYG 363
           IG+F AM+ G+ V+C+AGN+GP ++SVANVAPW+ T+GA TLDR FPA   L NG+ + G
Sbjct: 307 IGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTG 366

Query: 364 ESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEKGQ 423
            S+Y G      TK LE+VY  G      LCL GSL    V GKIV+CDRGVN R EKG 
Sbjct: 367 VSLYSG--VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 426

Query: 424 IVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQFGG 483
           +V+++GG  MI+ANTA + EE + D H+LPA  +G    + L+ Y+ + S P A + F G
Sbjct: 427 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 486

Query: 484 TVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNF 543
           TV+    +P VA FSSRGP+   P  LKPDVI PGVNI+A W   +GPTGL +DSRR+ F
Sbjct: 487 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 546

Query: 544 TVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDG--SKPAG 603
            +MSGTSM+CPH+SG+A L+ +AHP+W+P+AIKSA+MTTA V D+   P+ D   +  + 
Sbjct: 547 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 606

Query: 604 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHKVLQMN 663
            +A G+GH++P KA+ PGLVYDI   EY+  LC+L YT   I  I+   +V+C K  + +
Sbjct: 607 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFS 666

Query: 664 KGFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKY 723
               LNYPS SV+F  K   +  +R +TNVG+ +S+Y V V     V + VKP +L FK 
Sbjct: 667 DPGQLNYPSFSVLFGGKRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 726

Query: 724 VNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
           V +   Y V F+S+KG    T  +  G +TW    N +++VRSP+  +W
Sbjct: 727 VGEKKRYTVTFVSKKG-VSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Csor.00g013770 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 641.0 bits (1652), Expect = 1.3e-183
Identity = 360/775 (46.45%), Postives = 489/775 (63.10%), Query Frame = 0

Query: 5   SQMLLLSTLF---LCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQTL 64
           S   L ST F   LC     V ++     TYI+ +    + SS F     W+ S L    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI- 61

Query: 65  SAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLG 124
                 S+ LLY+Y NA+ GF+ +L++ E + L   P V++V  + +YE+ TT +P FLG
Sbjct: 62  ----SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 121

Query: 125 LSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSN 184
           L   +  ++  +      +VGVLDTGVWPES S+SD    PIP  W+G C+ G +F +S 
Sbjct: 122 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 181

Query: 185 CNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNG 244
           CNRKLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  
Sbjct: 182 CNRKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 241

Query: 245 AGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSI 304
           +G A+GMAPRA +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +
Sbjct: 242 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 301

Query: 305 AIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIY 364
           AIG+F AM+ GI V+C+AGN GP  SS++NVAPWITT+GA TLDR FPA+  L NG+   
Sbjct: 302 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 361

Query: 365 GESMYPGNKFKHATKELEVVYL--TGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSE 424
           G S++ G       K L  +Y         G LC+ G+L  E+V+GKIV+CDRG+N R +
Sbjct: 362 GVSLFKGEAL--PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 421

Query: 425 KGQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQ 484
           KG +VK +GG  MILANTA N EE + D H+LPAT +G    + ++ Y+ T  NP A I 
Sbjct: 422 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 481

Query: 485 FGGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRR 544
             GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPTGL  DSRR
Sbjct: 482 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 541

Query: 545 SNFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILD--GSK 604
             F ++SGTSM+CPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T   GKP+LD    K
Sbjct: 542 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 601

Query: 605 PAGVFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQ 664
           P+  F  GAGH++PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C     
Sbjct: 602 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC----D 661

Query: 665 MNKGFS---LNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTA-PKGVQVRVKPR 724
            +K +S   LNYPS +V        K  +R +T+VG   + YSV+VT+   GV++ V+P 
Sbjct: 662 PSKSYSVADLNYPSFAVNVDGVGAYK-YTRTVTSVGGAGT-YSVKVTSETTGVKISVEPA 721

Query: 725 RLVFKYVNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
            L FK  N+  +Y V F  +  K   + S+S G + W    +GK+ V SP+ ++W
Sbjct: 722 VLNFKEANEKKSYTVTFTVDSSK--PSGSNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Csor.00g013770 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 636.7 bits (1641), Expect = 2.4e-182
Identity = 347/775 (44.77%), Postives = 483/775 (62.32%), Query Frame = 0

Query: 7   MLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFL----EQTLS 66
           + ++ ++ L FL  +    I T +TY+I +    +   P+ +  QW+ S +    +    
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAM-PLPYTNHLQWYSSKINSVTQHKSQ 71

Query: 67  AEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL 126
            EE +++R+LY+Y  A  G AAQL++ E E L++   VVAV  + +YE+ TT SP FLGL
Sbjct: 72  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 131

Query: 127 S-LGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSSN 186
               S+ VW         +VGVLDTG+WPES SF+D+ M P+P  WRGAC+ G+ F   N
Sbjct: 132 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 191

Query: 187 CNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNG 246
           CNRK++GA+ F +G+  A+ +  D + EY SPRD  GHGTHT++T AG+ V  A++FG  
Sbjct: 192 CNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 251

Query: 247 AGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSI 306
            G A+GMA +A +A YKVCW  GC+SSDI++A+D A+ DGV +LS+SLGG    +  DS+
Sbjct: 252 YGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSL 311

Query: 307 AIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFIY 366
           +I +F AM+ G+ V+C+AGN GP   S+ NV+PWITT+GAST+DR FPA V++       
Sbjct: 312 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 371

Query: 367 GESMYPGNKFKHATKELEVVYLTGGQMGGE---LCLKGSLPREQVEGKIVLCDRGVNGRS 426
           G S+Y G       K+  +VYL       +    CL G+L R  V GKIV+CDRGV  R 
Sbjct: 372 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 431

Query: 427 EKGQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARI 486
           +KGQ+VK +GG  M+L NTA N EE + D H+LPA  +G  E   +K Y  T+    A +
Sbjct: 432 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 491

Query: 487 QFGGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSR 546
           +  GT IG   +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++ P+ L  D R
Sbjct: 492 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 551

Query: 547 RSNFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILD--GS 606
           R  F ++SGTSM+CPHVSG+AALI S HP W+PAAIKSA+MTTA V D+  KP+ D  G+
Sbjct: 552 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 611

Query: 607 KPAGVFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIIT-HMNVSCHKV 666
            P+  +  GAGHI+P +A DPGLVYDI P EY   LC    + S++ + T H N +C   
Sbjct: 612 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 671

Query: 667 LQMNKGFSLNYPSMSVVFKHKTTSKAVS--RRLTNVGSPNSIYSVEVTAPKGVQVRVKPR 726
           L  N G +LNYP++S +F   T  KA++  R +TNVG   S Y V V+  KG  V V+P+
Sbjct: 672 LAKNPG-NLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 731

Query: 727 RLVFKYVNQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTW 769
            L F   +Q L+Y V F +      +      G L W   ++  +KVRSP+++TW
Sbjct: 732 TLNFTSKHQKLSYTVTFRT----RFRMKRPEFGGLVW---KSTTHKVRSPVIITW 776

BLAST of Csor.00g013770 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 632.9 bits (1631), Expect = 3.4e-181
Identity = 341/755 (45.17%), Postives = 480/755 (63.58%), Query Frame = 0

Query: 31  TYIIQLHPQGLTSSPFYSKRQWHLSFLEQTLSAEEDSSSRLLYSYSNAMEGFAAQLSETE 90
           TYI+ +  +    S F +   W+ S    +L++   S   ++++Y     GF+A+L+  +
Sbjct: 27  TYIVHVDHEA-KPSIFPTHFHWYTS----SLASLTSSPPSIIHTYDTVFHGFSARLTSQD 86

Query: 91  IEYLKKLPDVVAVRADRKYEIQTTYSPKFLGL-SLGSQGVWFNSSMGEGAIVGVLDTGVW 150
              L   P V++V  ++   + TT SP+FLGL S    G+   S  G   ++GV+DTGVW
Sbjct: 87  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 146

Query: 151 PESPSFSDSRMPPIPQKWRGACQEGQDFNSSNCNRKLIGAKFFIKGHHVASSQPSDVDQE 210
           PE PSF D  + P+P KW+G C   QDF  S CNRKL+GA+FF  G+   + + ++   E
Sbjct: 147 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNET-TE 206

Query: 211 YVSPRDSHGHGTHTSSTAAGASVAEASVFGNGAGVAQGMAPRAHIAVYKVCWFSGCYSSD 270
           + SPRDS GHGTHT+S +AG  V  AS  G   GVA GMAP+A +A YKVCW SGCY SD
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSD 266

Query: 271 IVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVNCAAGNNGPIQSSV 330
           I+AA D A+ DGVD++SLS+GG  +P++ D+IAIG+F A+  GI V+ +AGN GP   +V
Sbjct: 267 ILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTV 326

Query: 331 ANVAPWITTIGASTLDRRFPAIVRLSNGEFIYGESMYPGNKFKHATKELEVVYLTGGQMG 390
            NVAPW+TT+GA T+DR FPA V+L NG+ I G S+Y G       +   +VY  G  +G
Sbjct: 327 TNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPG-RMYPLVY-GGSLLG 386

Query: 391 GE-----LCLKGSLPREQVEGKIVLCDRGVNGRSEKGQIVKESGGAAMILANTAINLEED 450
           G+     LCL+GSL    V+GKIVLCDRG+N R+ KG+IV+++GG  MI+AN   + E  
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 451 LVDVHVLPATLIGFAEANRLKAYMNTTS------NPKARIQFGGTVIGRSRAPSVAQFSS 510
           + D HVLPAT +G +  + ++ Y++ +S      +P A I F GT +G   AP VA FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 511 RGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTVMSGTSMACPHVSGI 570
           RGP+   P  LKPDVIAPG+NI+AAWP  +GP+G+  D+RR+ F ++SGTSMACPHVSG+
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 571 AALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGS--KPAGVFAMGAGHINPTKAID 630
           AAL+ +AHP W+PAAI+SA++TTA   D+ G+P++D S    + V   G+GH++PTKA+D
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 631 PGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFSLNYPSMSVVFKHK 690
           PGLVYDI  Y+Y+  LC   YT + I  IT     C    +     +LNYPS SVVF+  
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 691 TTSKAVS---RRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYVNQSLNYKVWFMSE 750
             SK  +   R +TNVG  +S+Y +++  P+G  V V+P +L F+ V Q L++ V   + 
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 751 KGKEGKTVSSSE-GHLTWVHSENGKYKVRSPIVVT 768
           + K     ++ E GH+ W    +GK  V SP+VVT
Sbjct: 747 EVKLSPGATNVETGHIVW---SDGKRNVTSPLVVT 770

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644951.1e-30166.23Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 S... [more]
Q9ZUF65.1e-19047.72Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
O653511.8e-18246.45Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9FLI43.3e-18144.77Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Q9LUM34.8e-18045.17Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6606345.10.0100.00Subtilisin-like protease 1.2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036287.10.099.87Subtilisin-like protease SBT1.2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022930926.10.099.35subtilisin-like protease SBT1.2 [Cucurbita moschata][more]
XP_022995265.10.098.57subtilisin-like protease SBT1.2 [Cucurbita maxima][more]
XP_023532819.10.098.18subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1ES950.099.35subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111437268 PE... [more]
A0A6J1K3M40.098.57subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111490865 PE=3... [more]
A0A0A0LDY70.089.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=3 SV=1[more]
A0A5A7T5340.090.00Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BHA20.090.00subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G04110.17.9e-30366.23Subtilase family protein [more]
AT2G05920.13.7e-19147.72Subtilase family protein [more]
AT5G67360.11.3e-18346.45Subtilase family protein [more]
AT5G51750.12.4e-18244.77subtilase 1.3 [more]
AT3G14240.13.4e-18145.17Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 136..155
score: 37.28
coord: 546..562
score: 60.63
coord: 215..228
score: 44.04
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 31..111
e-value: 3.7E-16
score: 59.6
IPR003137PA domainPFAMPF02225PAcoord: 379..464
e-value: 1.9E-9
score: 37.4
NoneNo IPR availableGENE3D3.50.30.30coord: 345..485
e-value: 1.6E-179
score: 599.5
NoneNo IPR availableGENE3D2.60.40.2310coord: 632..768
e-value: 1.4E-35
score: 123.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..226
NoneNo IPR availablePANTHERPTHR10795:SF350SUBTILISIN-LIKE PROTEASE SBT1.2coord: 26..767
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 114..615
score: 31.587418
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 352..478
e-value: 9.75358E-36
score: 129.456
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 51..111
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 136..586
e-value: 1.1E-50
score: 173.0
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 19..111
e-value: 8.4E-19
score: 69.8
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 664..766
e-value: 8.1E-26
score: 90.1
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 139..628
e-value: 1.6E-179
score: 599.5
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 112..619
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 26..767
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 547..557
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 109..582
e-value: 2.92952E-148
score: 433.948

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g013770.m01Csor.00g013770.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0032440 2-alkenal reductase [NAD(P)+] activity
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity