CsaV3_7G030010 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_7G030010
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionProtein kinase domain-containing protein
Locationchr7: 18978537 .. 18979816 (+)
RNA-Seq ExpressionCsaV3_7G030010
SyntenyCsaV3_7G030010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CACATATTCATTCATCCTTTTCAAGAGCTTTCTGTTTCAACAATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGTGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATAAGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGACATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAAATGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAGTAGATTTTGTAATCTTGAAGTTTAATTAAAGCAATTAACTATGCATTCCATTGTTTATGATTATTGATAAATGGCAAATTATTTAACTCGTTTGATCCAATTGTTGGGTAATCTTTCTTCACTTTATGAACAAACAAACAGCATGCAAGTCTAAATTCAATTTGTTTGAATGAAAA

mRNA sequence

ATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGTGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATAAGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGACATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAAATGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAG

Coding sequence (CDS)

ATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGTGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATAAGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGACATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAAATGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAG

Protein sequence

MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN*
Homology
BLAST of CsaV3_7G030010 vs. NCBI nr
Match: XP_004136151.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 hypothetical protein Csa_015671 [Cucumis sativus])

HSP 1 Score: 730.7 bits (1885), Expect = 6.0e-207
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60
           MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF
Sbjct: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120
           TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM
Sbjct: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120

Query: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180
           ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK
Sbjct: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180

Query: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240
           YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK
Sbjct: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240

Query: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300
           LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK
Sbjct: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300

Query: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 354
           LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN
Sbjct: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 353

BLAST of CsaV3_7G030010 vs. NCBI nr
Match: KAA0046699.1 (serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1 serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 305/353 (86.40%), Postives = 323/353 (91.50%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLLWEEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
            S+EIP IP+ELSKQG DFLEKCFVR+Y+QRWTADMLLQHPYLNE+NKDT + DEKLKLP
Sbjct: 242 NSKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSD-DEKLKLP 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLK-PSKAIFLPHQFFKSKA-QSCNN 354
           K   FLPHQFF  K DML    KNGDEKL+ P KAIFLPHQFF S A QSCN+
Sbjct: 302 KT-AFLPHQFFNRKADML----KNGDEKLELPKKAIFLPHQFFNSTAQQSCND 346

BLAST of CsaV3_7G030010 vs. NCBI nr
Match: XP_008451652.1 (PREDICTED: serine/threonine-protein kinase Chk1-like [Cucumis melo])

HSP 1 Score: 474.9 bits (1221), Expect = 5.9e-130
Identity = 239/290 (82.41%), Postives = 256/290 (88.28%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLLWEEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQ-HPYLN--EEN 290
            S+EIP IP+ELSKQ         +  Y + +     LQ + YL+  EEN
Sbjct: 242 NSKEIPIIPKELSKQASSSQWLQALEKYSKTFLPSSSLQVYIYLSSFEEN 289

BLAST of CsaV3_7G030010 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 459.5 bits (1181), Expect = 2.6e-125
Identity = 234/346 (67.63%), Postives = 273/346 (78.90%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTD 62
           ++WVLVK +G GS G+VCLAKQ+TKE+SD HYYFAVK  SL+   SSLLWEE VLKHF D
Sbjct: 2   ENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQ-RKSSLLWEEQVLKHFKD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQ LGSE+T  GD  DD  LYNLKLEYA GGTLADLIKQR KLPEDEVK YL+MIL
Sbjct: 62  CPEIVQCLGSEIT-FGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHR+GFVHVDLKP N+L FPQSDGKMKLKIADFGLAER CK   DDQ DRG    
Sbjct: 122 KGLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAER-CKHIVDDQ-DRGY--- 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
               K + T +YMSPESI F+E+NG HDIWSLGC LV+MVSG+ VW+DC+  E+L+T+LL
Sbjct: 182 --LRKFKGTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
             + IPTIP+ELSKQGKDF+++CF+RNY+QRWTADMLLQHPYLNEEN+ TK GD+ +KL 
Sbjct: 242 NDKNIPTIPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDDIVKLI 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKA 349
           KAI  LPHQFF+ K         + +EK    K  +LPH  +K+K+
Sbjct: 302 KAI-RLPHQFFKRKA------KSSNNEKSNRQKEDYLPHLLYKNKS 331

BLAST of CsaV3_7G030010 vs. NCBI nr
Match: XP_004137063.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 hypothetical protein Csa_017187 [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 7.8e-106
Identity = 205/321 (63.86%), Postives = 250/321 (77.88%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 62
           ++WVLVK LG+GS GLVCLAKQ T+++  ++LHYYFAVK + L +NSS  LWEE VLK F
Sbjct: 2   ENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS--LWEELVLKQF 61

Query: 63  TDCPEIVQYLGSEVT---GGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 122
            +CPEIVQYLGSE+T   GG D   DK+ Y LKLEYAAGGTL DLIKQ+ KLPEDEVK+Y
Sbjct: 62  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 121

Query: 123 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGED----- 182
           L+MILKGLSCIH KGFVHVDLKP NILAFP+S+GKMKLKIADFG AER CK   D     
Sbjct: 122 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAER-CKYRNDADAHS 181

Query: 183 DQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCK 242
           D E R S+  S  LK + T RYMSPESIVF E+N  HD+WSLGC LV+M+SG+  W++  
Sbjct: 182 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 241

Query: 243 SYEELITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDT 302
           + ++L+ K+L S+ +P IPEELS QGKDF++KCFV N++QRWTADMLLQHPYLNE+++ T
Sbjct: 242 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 301

Query: 303 --KNGDEKLKLPKAIVFLPHQ 312
             K+ D   KL K+++ L HQ
Sbjct: 302 TMKDDDGMPKLAKSLL-LSHQ 318

BLAST of CsaV3_7G030010 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-45
Identity = 113/313 (36.10%), Postives = 175/313 (55.91%), Query Frame = 0

Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63
           +WV  + +G G+   V  A + ++   D     AVK     Y ++SL  E+ VL    DC
Sbjct: 2   EWVRGETIGFGTFSTVSTATK-SRNSGDFPALIAVKSTD-AYGAASLSNEKSVLDSLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQMI 123
           PEI++  G + T      + +E++NL LEYA+ G+LA  +K+     LPE  V+++   +
Sbjct: 62  PEIIRCYGEDST----VENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSV 121

Query: 124 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKY 183
           L+GL  IH KGF H D+K  NIL F  +DG   +KIADFGLA R          D     
Sbjct: 122 LRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR---------VDGDLTA 181

Query: 184 YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELIT 243
              ++++R T  YM+PE +  +E     D+W+LGC +V+M SG+  W+  +   +  L+ 
Sbjct: 182 LRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLI 241

Query: 244 KLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKD-------- 303
           ++ + +E+P IPE LS++GKDFL KCFV++  +RWTA+MLL H ++  + +D        
Sbjct: 242 RIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVV 295

BLAST of CsaV3_7G030010 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.3e-33
Identity = 99/286 (34.62%), Postives = 136/286 (47.55%), Query Frame = 0

Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63
           +W   + LG GS   V  A     +E       AVK  S   + S  L  E  +      
Sbjct: 2   EWTRGRILGRGSTATVYAAAGHNSDE-----ILAVK--SSEVHRSEFLQREAKILSSLSS 61

Query: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLAD-LIKQRNKLPEDEVKKYLQMIL 123
           P ++ Y GSE     + +    +YNL +EYA  GTL D   K   ++ E  V KY + IL
Sbjct: 62  PYVIGYRGSETKRESNGV---VMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDIL 121

Query: 124 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 183
           KGL  IH KG VH D+K  N++       K + KIADFG A+R     E           
Sbjct: 122 KGLEYIHSKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKRVDPVFES---------- 181

Query: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 243
                V  T  +M+PE     +     DIW++GCT+++MV+G   W    S E+ ++ L 
Sbjct: 182 ----PVMGTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLY 241

Query: 244 ---ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYL 286
               S E P +P  L+++ KDFLEKC  R   +RWTA  LL HP+L
Sbjct: 242 RVGYSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of CsaV3_7G030010 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.8e-31
Identity = 89/301 (29.57%), Postives = 153/301 (50.83%), Query Frame = 0

Query: 5   WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSS----------LLWEE 64
           W   + +G G+ G V +   +   E       A+K  S+  N +S          L  E 
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGE-----LLAIKEVSIAMNGASRERAQAHVRELEEEV 139

Query: 65  HVLKHFTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEV 124
           ++LK+ +  P IV+YLG+    G          N+ LE+  GG+++ L+ +    PE  +
Sbjct: 140 NLLKNLSH-PNIVRYLGTAREAGS--------LNILLEFVPGGSISSLLGKFGSFPESVI 199

Query: 125 KKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQ 184
           + Y + +L GL  +H+ G +H D+K  NIL     D K  +K+ADFG +++  +      
Sbjct: 200 RMYTKQLLLGLEYLHKNGIMHRDIKGANILV----DNKGCIKLADFGASKKVVELAT--- 259

Query: 185 EDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSY 244
                   +GA  ++ T  +M+PE I+ +  +   DIWS+GCT+++M +G+  W+  + Y
Sbjct: 260 -------MTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWS--QQY 319

Query: 245 EELITKLLI--SEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDT 294
           +E+     I  ++  P IPE LS + KDFL KC  +    R +A  LLQHP++  E+++ 
Sbjct: 320 QEVAALFHIGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEA 350

BLAST of CsaV3_7G030010 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.8e-31
Identity = 99/289 (34.26%), Postives = 149/289 (51.56%), Query Frame = 0

Query: 5   WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASL-------RYNSSSLLWEEHVL 64
           W   + LG GS G V L      E  ++    A+K  +L       R ++  L  E  VL
Sbjct: 400 WKKGRLLGMGSFGHVYLG--FNSESGEM---CAMKEVTLCSDDPKSRESAQQLGQEISVL 459

Query: 65  KHFTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 124
                   IVQY GSE       +DDK LY + LEY +GG++  L+++  +  E+ ++ Y
Sbjct: 460 SRLRH-QNIVQYYGSET------VDDK-LY-IYLEYVSGGSIYKLLQEYGQFGENAIRNY 519

Query: 125 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDR 184
            Q IL GL+ +H K  VH D+K  NIL  P      ++K+ADFG+A+    +        
Sbjct: 520 TQQILSGLAYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMAKHITAQ-------- 579

Query: 185 GSKYYSGALKVRATHRYMSPESIVFSEINGLH-DIWSLGCTLVQMVSGERVWNDCKSYEE 244
                SG L  + +  +M+PE I  S  + L  DIWSLGCT+++M + +  W+  +    
Sbjct: 580 -----SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPA 639

Query: 245 LITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYL 286
           +  K+  S+E+P IP+ LS++GKDF+ KC  RN   R TA  LL H ++
Sbjct: 640 MF-KIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of CsaV3_7G030010 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 97/295 (32.88%), Postives = 153/295 (51.86%), Query Frame = 0

Query: 5    WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASL-RYNS---------SSLLWEE 64
            W+  + +G+GS G V L   +T  E       AVK+  + +Y+S          +L  E 
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVTTGE-----MMAVKQVEVPKYSSQNEAILSTVEALRSEV 1234

Query: 65   HVLKHFTDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEV 124
              LK   D   IVQYLG        F +   +Y+L LEY AGG++  LI+   +  E  +
Sbjct: 1235 STLKDL-DHLNIVQYLG--------FENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLI 1294

Query: 125  KKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQ 184
            K     +LKGL+ +H KG +H D+K DN+L     DG    KI+DFG++ +S        
Sbjct: 1295 KHLTTQVLKGLAYLHSKGILHRDMKADNLLL--DQDG--ICKISDFGISRKS-------- 1354

Query: 185  EDRGSKYYSGALKVRATHRYMSPESIVFSE-INGLHDIWSLGCTLVQMVSGERVWNDCKS 244
                  Y +  + +R T  +M+PE +   +  +   DIWSLGC +++M +G+R W++ + 
Sbjct: 1355 ---KDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNLEV 1414

Query: 245  YEELITKLLISEEIPTIPEE----LSKQGKDFLEKCFVRNYEQRWTADMLLQHPY 285
               +  K+  S+  P IPE+    +S+ G++FL+ CF  N E+R TA+ LL HP+
Sbjct: 1415 VAAMF-KIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPF 1439

BLAST of CsaV3_7G030010 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 2.9e-207
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60
           MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF
Sbjct: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120
           TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM
Sbjct: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120

Query: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180
           ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK
Sbjct: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180

Query: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240
           YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK
Sbjct: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240

Query: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300
           LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK
Sbjct: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300

Query: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 354
           LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN
Sbjct: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 353

BLAST of CsaV3_7G030010 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.7e-171
Identity = 305/353 (86.40%), Postives = 323/353 (91.50%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLLWEEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
            S+EIP IP+ELSKQG DFLEKCFVR+Y+QRWTADMLLQHPYLNE+NKDT + DEKLKLP
Sbjct: 242 NSKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSD-DEKLKLP 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLK-PSKAIFLPHQFFKSKA-QSCNN 354
           K   FLPHQFF  K DML    KNGDEKL+ P KAIFLPHQFF S A QSCN+
Sbjct: 302 KT-AFLPHQFFNRKADML----KNGDEKLELPKKAIFLPHQFFNSTAQQSCND 346

BLAST of CsaV3_7G030010 vs. ExPASy TrEMBL
Match: A0A1S3BRE6 (serine/threonine-protein kinase Chk1-like OS=Cucumis melo OX=3656 GN=LOC103492884 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.9e-130
Identity = 239/290 (82.41%), Postives = 256/290 (88.28%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLLWEEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQ-HPYLN--EEN 290
            S+EIP IP+ELSKQ         +  Y + +     LQ + YL+  EEN
Sbjct: 242 NSKEIPIIPKELSKQASSSQWLQALEKYSKTFLPSSSLQVYIYLSSFEEN 289

BLAST of CsaV3_7G030010 vs. ExPASy TrEMBL
Match: A0A0A0K7G3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065150 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 3.8e-106
Identity = 205/321 (63.86%), Postives = 250/321 (77.88%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 62
           ++WVLVK LG+GS GLVCLAKQ T+++  ++LHYYFAVK + L +NSS  LWEE VLK F
Sbjct: 2   ENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS--LWEELVLKQF 61

Query: 63  TDCPEIVQYLGSEVT---GGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 122
            +CPEIVQYLGSE+T   GG D   DK+ Y LKLEYAAGGTL DLIKQ+ KLPEDEVK+Y
Sbjct: 62  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 121

Query: 123 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGED----- 182
           L+MILKGLSCIH KGFVHVDLKP NILAFP+S+GKMKLKIADFG AER CK   D     
Sbjct: 122 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAER-CKYRNDADAHS 181

Query: 183 DQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCK 242
           D E R S+  S  LK + T RYMSPESIVF E+N  HD+WSLGC LV+M+SG+  W++  
Sbjct: 182 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 241

Query: 243 SYEELITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDT 302
           + ++L+ K+L S+ +P IPEELS QGKDF++KCFV N++QRWTADMLLQHPYLNE+++ T
Sbjct: 242 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 301

Query: 303 --KNGDEKLKLPKAIVFLPHQ 312
             K+ D   KL K+++ L HQ
Sbjct: 302 TMKDDDGMPKLAKSLL-LSHQ 318

BLAST of CsaV3_7G030010 vs. ExPASy TrEMBL
Match: A0A5D3C4R6 (Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002080 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 2.4e-105
Identity = 209/351 (59.54%), Postives = 258/351 (73.50%), Query Frame = 0

Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 62
           + WV VK LG+GS G+V LAKQ  + +   +LHYYFAVK + LR+N SSLLWEE VLK F
Sbjct: 2   EKWVFVKVLGQGSDGVVWLAKQTIRRKIGRNLHYYFAVKLSPLRHN-SSLLWEEQVLKQF 61

Query: 63  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 122
            +CPEIVQYLGSE+T GGD   DK+ Y LKLEYAAGGTL DLIKQ+ KLPE+EVK YL+M
Sbjct: 62  KNCPEIVQYLGSEITRGGDLPQDKDFYTLKLEYAAGGTLDDLIKQKEKLPENEVKDYLRM 121

Query: 123 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCK--RGEDDQEDRG 182
           ILKGLSC+H KGFVHVDLKP+NILAFPQSDGKMKLKIADFG AER CK  R +DD +D+ 
Sbjct: 122 ILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAER-CKLYRNDDDDDDQD 181

Query: 183 SKY--YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEE 242
            KY  Y  +LK + T RYMSPESIV +E+N  HDIWSLGC LV+M+SG+ VW+D     +
Sbjct: 182 RKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDDLIDSRQ 241

Query: 243 LITKLLISE--EIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYL-NEENK--D 302
           L+ +LL ++   +PTIP+ELSKQGKDF++KCF+RN +QRWTADMLLQHPYL +EEN+   
Sbjct: 242 LMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHEENEAAT 301

Query: 303 TKNGDEKL--KLPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLP 341
           T   D+++   L KA+     +  +S+T        N  +    +K   LP
Sbjct: 302 TVKDDDQMPTTLAKALSLSRQRPLKSQTRSSNNNNNNNRKFNLRAKNYLLP 350

BLAST of CsaV3_7G030010 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 195.7 bits (496), Expect = 6.4e-50
Identity = 116/299 (38.80%), Postives = 169/299 (56.52%), Query Frame = 0

Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63
           +W+  + +G GS   V LA              AVK + +   S++L  E  VL    DC
Sbjct: 2   EWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGV-VCSAALRNERDVLDDLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNK-LPEDEVKKYLQMIL 123
            EIV+  G     G    + +E+YNL LEYA+GG+LAD IK   + LPE EV+++ + I+
Sbjct: 62  SEIVRCFGE----GRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIV 121

Query: 124 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 183
           KGL  IH  GF H D+K +N+L F   D    +KI+DFGLA+R  + GE           
Sbjct: 122 KGLCHIHGNGFTHCDIKLENVLVFGDGD----VKISDFGLAKR--RSGE----------- 181

Query: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVW----NDCKSYEELI 243
              +++R T  YM+PES+   E     DIW+LGC++V+M SG+  W        +   L+
Sbjct: 182 -VCVEIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLL 241

Query: 244 TKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDE 298
            ++   +E+P IP ELS++GKDF+ KCFV+N  +RWTA+MLL HP+L     D ++G+E
Sbjct: 242 VRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL---AVDDESGEE 274

BLAST of CsaV3_7G030010 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 186.8 bits (473), Expect = 3.0e-47
Identity = 108/287 (37.63%), Postives = 169/287 (58.89%), Query Frame = 0

Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF-TD 63
           +W+  + +G G+   V LA +   +  +     AVK A   Y ++SL  E+ VL +   D
Sbjct: 2   EWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSAD-SYGAASLANEKSVLDNLGDD 61

Query: 64  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQM 123
           C EIV+  G + T      + +E++NL LEYA+ G+L   +K+     +PE  V+++   
Sbjct: 62  CNEIVRCFGEDRT----VENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGS 121

Query: 124 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 183
           +L+GL  IH  GF H DLK  NIL F    G   +KIADFGLA+R          D  + 
Sbjct: 122 VLRGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRI--------GDLTAL 181

Query: 184 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELI 243
            Y   +++R T  YM+PES+  +E     D+W+LGC +V+M SG+  W+  +  ++  L+
Sbjct: 182 NY--GVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLL 241

Query: 244 TKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYL 286
            ++ + +E+P IPEELS+QG+DFL KCFV++ ++RWTA+MLL HP++
Sbjct: 242 LRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFV 269

BLAST of CsaV3_7G030010 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 184.5 bits (467), Expect = 1.5e-46
Identity = 113/313 (36.10%), Postives = 175/313 (55.91%), Query Frame = 0

Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63
           +WV  + +G G+   V  A + ++   D     AVK     Y ++SL  E+ VL    DC
Sbjct: 2   EWVRGETIGFGTFSTVSTATK-SRNSGDFPALIAVKSTD-AYGAASLSNEKSVLDSLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQMI 123
           PEI++  G + T      + +E++NL LEYA+ G+LA  +K+     LPE  V+++   +
Sbjct: 62  PEIIRCYGEDST----VENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSV 121

Query: 124 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKY 183
           L+GL  IH KGF H D+K  NIL F  +DG   +KIADFGLA R          D     
Sbjct: 122 LRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR---------VDGDLTA 181

Query: 184 YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELIT 243
              ++++R T  YM+PE +  +E     D+W+LGC +V+M SG+  W+  +   +  L+ 
Sbjct: 182 LRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLI 241

Query: 244 KLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKD-------- 303
           ++ + +E+P IPE LS++GKDFL KCFV++  +RWTA+MLL H ++  + +D        
Sbjct: 242 RIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVV 295

BLAST of CsaV3_7G030010 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 176.4 bits (446), Expect = 4.0e-44
Identity = 110/313 (35.14%), Postives = 163/313 (52.08%), Query Frame = 0

Query: 2   DQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFT 61
           +Q+W+    +G GS   V L   IT        +FAVK  S  ++SS+ L  E  +    
Sbjct: 3   EQNWIRGPIIGRGSTATVSLG--ITNSGD----FFAVK--SAEFSSSAFLQREQSILSKL 62

Query: 62  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQR-NKLPEDEVKKYLQM 121
             P IV+Y+GS VT      +DK +YNL +EY +GG+L DLIK    KLPE  ++ Y + 
Sbjct: 63  SSPYIVKYIGSNVTKE----NDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQ 122

Query: 122 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 181
           ILKGL  +H +G VH D+K  N++      G    KI D G     C +  ++ E+    
Sbjct: 123 ILKGLMYLHDQGIVHCDVKSQNVMI-----GGEIAKIVDLG-----CAKTVEENEN---- 182

Query: 182 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 241
                L+   T  +MSPE     E +   D+W+LGCT+++M +G   W +       I K
Sbjct: 183 -----LEFSGTPAFMSPEVARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYK 242

Query: 242 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 301
           +  + E P IP  LS++G+DFL KC  ++ +QRWT + LLQHP+L+EE+ D+      L 
Sbjct: 243 IGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLN 284

Query: 302 LPKAIVFLPHQFF 314
                  L  +F+
Sbjct: 303 SSSPSTVLDQRFW 284

BLAST of CsaV3_7G030010 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.5e-43
Identity = 115/301 (38.21%), Postives = 163/301 (54.15%), Query Frame = 0

Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60
           M  +   VK LG+G+ G V L     K+      Y AVK      N  S+  E  +L   
Sbjct: 1   MSPEMEFVKVLGKGTYGSVELFSH--KQNDGSLLYNAVKIMDSE-NYGSIDQEFRILSEL 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQ-RNKLPEDEVKKYLQ 120
             CP IVQ  G+ +  G D  + K++Y + +EYAA GTL + IK+ R KL +  +K + +
Sbjct: 61  RGCPCIVQLCGNSLVQGID-CNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTR 120

Query: 121 MILKGLSCIHRKGFVHVDLKPDNILAFPQSDG-----KMKLKIADFGLAERSCKRGEDDQ 180
           MIL+GL  IH  G+VH DLKPDNIL FP  D        +LKI+DFG++ R+        
Sbjct: 121 MILQGLVSIHNHGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRA-------- 180

Query: 181 EDRGSKYYSGALKVR----ATHRYMSPESIV-FSEINGLHDIWSLGCTLVQMVSGERVWN 240
              G K  SG  +V      T  YMSPES+   + +    D+WSLGC +++M +G+R W 
Sbjct: 181 ---GDK--SGCWRVDEPWVGTSIYMSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPW- 240

Query: 241 DCKSYEELITKLLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEEN 291
               +E+ +  LL++++ P IPE L    + FLEKCF R  E+R +A  LL HP+L  + 
Sbjct: 241 --LGFEKDVKSLLLNQKAPEIPETLPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDE 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004136151.16.0e-207100.00mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 ... [more]
KAA0046699.12.0e-17086.40serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1... [more]
XP_008451652.15.9e-13082.41PREDICTED: serine/threonine-protein kinase Chk1-like [Cucumis melo][more]
XP_038896325.12.6e-12567.63mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_004137063.17.8e-10663.86mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 ... [more]
Match NameE-valueIdentityDescription
Q9SND62.1e-4536.10Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808885.3e-3334.62Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q405413.8e-3129.57Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Q9CAD53.8e-3134.26Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Q013891.1e-3032.88Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
A0A0A0K8352.9e-207100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407... [more]
A0A5A7TZP49.7e-17186.40Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BRE62.9e-13082.41serine/threonine-protein kinase Chk1-like OS=Cucumis melo OX=3656 GN=LOC10349288... [more]
A0A0A0K7G33.8e-10663.86Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065... [more]
A0A5D3C4R62.4e-10559.54Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT4G36950.16.4e-5038.80mitogen-activated protein kinase kinase kinase 21 [more]
AT5G67080.13.0e-4737.63mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.11.5e-4636.10mitogen-activated protein kinase kinase kinase 20 [more]
AT5G55090.14.0e-4435.14mitogen-activated protein kinase kinase kinase 15 [more]
AT2G41930.14.5e-4338.21Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 5..285
e-value: 6.7E-55
score: 198.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 7..285
e-value: 3.8E-49
score: 167.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 5..285
score: 38.138493
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 1..349
e-value: 3.6E-58
score: 199.3
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 6..277
e-value: 9.3E-14
score: 48.9
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 4..306
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 4..306
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_7G030010.1CsaV3_7G030010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity