CsaV3_7G023130 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_7G023130
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	glucan endo-1,3-beta-glucosidase 3-like
Location	chr7: 12045810 .. 12047655 (-)
RNA-Seq Expression	CsaV3_7G023130
Synteny	CsaV3_7G023130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCAAGATTTTTACAGGCTAATATGAACTTCCAACAACTTTTACTTCACTGCACTTTTTGTCTACTGATGATGACTTTTAACTGAGCTTTCTTGTGTCTTCATCAAGATTTATGTTGTTTTGTTCAATGTGCAATTCTGGTTCAAGAGGATGAGTTTATTTTTATTTCTAAAATAACAATGTGGGGGAAAAATATAGTTTAAATTTTGTTTCTGGGATCCAAAATGACAGGACACACGAGAGAGCTAAACCATGAGAATCAGAAATTCAGTTCCCGGCCAGTTTTCACCTCTCAAAGGGATATCACCACCCCTATAACAACGGTTCCCACAATAATTCTTTCCAACCCCACCCCCTCGACTCCATTTATCAATCCAACTTCAACTTCAGACACGTATTCTTCGGCCATGGAGTCTCCCAAAAGATCATCTCCACCGTCGTCAGGGACTAGTTGGTGCATTGCTAGTCAAAATGCATCACGAAAGGTATTACAAATTGCTCTAGACTACACATGTGGCTATGGTGGTACTGATTGTTCAGCAATTCAAGCTGGTCAGAGGTGTTACAACCCAAACACCATTCATGATCATGCCTCCTACGCCTTCAATAGCTACTATCAAAAGAATCCTGTTCCAGATAGCTGTAACTTTGGTGGGACTGCTGTCATCACTAGCACCGACCCAAGTATGTTGCTTCCTTCAAAAGTTGTTTCTTTGTTACCTCACTAGATAATAATGTGTTATTTTTCTATTCTCTCTACCTTTCTTTTTTTCTTTTTGAGGAAGAGGTTAAGAGAAAGTGGAGAAGATCAGGCAAACTTATTATAGATAATCTGGTAACGAGTCCAAAGAATCAAAGAATTCAAATTGTTGCAATGCTAACTGAGAGTTTCTTCTGAAGGTCCAGAAGCTTTGAAAATATTTAAAACAGCAGCCGTCGTGAACAAACTCTATTCGTCCTCTTTCCCCTTCTTAAAACCTTCTACGAGAAATGTGGATATAAAGACAATTAGTCCTTTCTTACTTTGAAGTTAATGATCAAAACCCCAAATCTAATAGTCAGAGGAAATTCAGCCCCCACAGCGCACACACTTTTTCCCCTTTTCCAAGTTGGGTTGGTGAAGGAAGAAGGTTCGGTGTGTCTGTAGGGACTATACAGTGATGTGAATTATTGAAGCTATAATGGGCCGTATTGGAATTCTTCTTTCTGCAACTGCAAAATGTTTGAGGACATAAAACTTCATAGATTGACTCTGTTTCTCTTTCCATAAAGTCTTGTTTATGTCTTTTCTCACCACTATTTTATTCAGGTACTATGGCATGTGAGTACACATCCACTAGGTAAAATCCCCATCTTCCATTGCTAACATTTGCTTTCTTTCCTTTCCTGCTACCAACATTCATTTCCTAACAATGACACTGCCATTTTTCAACTCTTCAGCACAAGTTCATCAGTTCTAAACACCACAAGTTCCAAGGAATCAACCGTGTTTGGTGCTGTACCCTCAAGCCCAACTCCTTCAGCAGCAACCTGTCAAGAAATAAATGTTCTTCAACAACTATTGTTGTTGACAATTGTTTCTCTAAGATTATTACACACTAACTATCTTTATGGCTGATTGGGACAGTAAAACCAACTTAATTTCTTATAAAACTTACCATTTACTTCACACAATTGAAGTTGCTTGAAAGAACTGCAGAGATTCGGGTTCAGGAACAAACATTTCTGGGACATGTTTTTCAGCCTATTTGAAAAGTAAAGCAAATCTATAAAGAGCAAAGTGAAGATTTTTCAATGTTGTGTCGGATGGAGAACATATATGCACATGTCTTGTTGTTCATTGGGG

mRNA sequence

ATGACAGGACACACGAGAGAGCTAAACCATGAGAATCAGAAATTCAGTTCCCGGCCAGTTTTCACCTCTCAAAGGGATATCACCACCCCTATAACAACGGTTCCCACAATAATTCTTTCCAACCCCACCCCCTCGACTCCATTTATCAATCCAACTTCAACTTCAGACACGTATTCTTCGGCCATGGAGTCTCCCAAAAGATCATCTCCACCGTCGTCAGGGACTAGTTGGTGCATTGCTAGTCAAAATGCATCACGAAAGGTATTACAAATTGCTCTAGACTACACATGTGGCTATGGTGGTACTGATTGTTCAGCAATTCAAGCTGGTCAGAGGTGTTACAACCCAAACACCATTCATGATCATGCCTCCTACGCCTTCAATAGCTACTATCAAAAGAATCCTGTTCCAGATAGCTGTAACTTTGGTGGGACTGCTGTCATCACTAGCACCGACCCAAGTACTATGGCATGTGAGTACACATCCACTAGCACAAGTTCATCAGTTCTAAACACCACAAGTTCCAAGGAATCAACCGTGTTTGGTGCTGTACCCTCAAGCCCAACTCCTTCAGCAGCAACCTGTCAAGAAATAAATGTTCTTCAACAACTATTGTTGTTGACAATTGTTTCTCTAAGATTATTACACACTAACTATCTTTATGGCTGA

Coding sequence (CDS)

ATGACAGGACACACGAGAGAGCTAAACCATGAGAATCAGAAATTCAGTTCCCGGCCAGTTTTCACCTCTCAAAGGGATATCACCACCCCTATAACAACGGTTCCCACAATAATTCTTTCCAACCCCACCCCCTCGACTCCATTTATCAATCCAACTTCAACTTCAGACACGTATTCTTCGGCCATGGAGTCTCCCAAAAGATCATCTCCACCGTCGTCAGGGACTAGTTGGTGCATTGCTAGTCAAAATGCATCACGAAAGGTATTACAAATTGCTCTAGACTACACATGTGGCTATGGTGGTACTGATTGTTCAGCAATTCAAGCTGGTCAGAGGTGTTACAACCCAAACACCATTCATGATCATGCCTCCTACGCCTTCAATAGCTACTATCAAAAGAATCCTGTTCCAGATAGCTGTAACTTTGGTGGGACTGCTGTCATCACTAGCACCGACCCAAGTACTATGGCATGTGAGTACACATCCACTAGCACAAGTTCATCAGTTCTAAACACCACAAGTTCCAAGGAATCAACCGTGTTTGGTGCTGTACCCTCAAGCCCAACTCCTTCAGCAGCAACCTGTCAAGAAATAAATGTTCTTCAACAACTATTGTTGTTGACAATTGTTTCTCTAAGATTATTACACACTAACTATCTTTATGGCTGA

Protein sequence

MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFGAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG*

Homology

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BLAST of CsaV3_7G023130 vs. NCBI nr
Match: XP_004148305.3 (major pollen allergen Ole e 10 [Cucumis sativus] >KAE8646120.1 hypothetical protein Csa_015812 [Cucumis sativus])

HSP 1 Score: 428.7 bits (1101), Expect = 3.1e-116
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSS 60
           MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSS
Sbjct: 1   MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSS 60

Query: 61  AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH 120
           AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH
Sbjct: 61  AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH 120

Query: 121 DHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTV 180
           DHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTV
Sbjct: 121 DHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTV 180

Query: 181 FGAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           FGAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG
Sbjct: 181 FGAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 222

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BLAST of CsaV3_7G023130 vs. NCBI nr
Match: XP_031744909.1 (glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8646097.1 hypothetical protein Csa_016385 [Cucumis sativus])

HSP 1 Score: 397.9 bits (1021), Expect = 5.8e-107
Identity = 206/221 (93.21%), Postives = 213/221 (96.38%), Query Frame = 0


Query: 2   TGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSA 61
           +GHTRELNHENQKFSSRPVF SQRDITTPITTVPTIILSNPT STPFINPTSTSDTYS A
Sbjct: 129 SGHTRELNHENQKFSSRPVFISQRDITTPITTVPTIILSNPTVSTPFINPTSTSDTYSPA 188

Query: 62  MESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHD 121
           MESPKRSSPPSSG SWCIASQ+AS+KVLQIALDY CGYGGTDCSAIQAGQRCYNPNTIHD
Sbjct: 189 MESPKRSSPPSSGASWCIASQSASQKVLQIALDYACGYGGTDCSAIQAGQRCYNPNTIHD 248

Query: 122 HASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVF 181
           HASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSSVLNTT+SK STVF
Sbjct: 249 HASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSVLNTTNSKGSTVF 308

Query: 182 GAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           GAVPSSPTPSAATCQEINVLQ+LLLLTIVSLRL H NYLYG
Sbjct: 309 GAVPSSPTPSAATCQEINVLQRLLLLTIVSLRLFHINYLYG 349

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BLAST of CsaV3_7G023130 vs. NCBI nr
Match: XP_016900361.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 3-like [Cucumis melo] >XP_016900362.1 PREDICTED: glucan endo-1,3-beta-glucosidase 3-like [Cucumis melo] >XP_016900363.1 PREDICTED: glucan endo-1,3-beta-glucosidase 3-like [Cucumis melo])

HSP 1 Score: 389.8 bits (1000), Expect = 1.6e-104
Identity = 202/220 (91.82%), Postives = 210/220 (95.45%), Query Frame = 0


Query: 3   GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAM 62
           GH REL+HENQKFSSRPVFTSQRDITTPITTVPTIILSNPT STPFINPTSTSDTYS AM
Sbjct: 54  GHARELSHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTASTPFINPTSTSDTYSPAM 113

Query: 63  ESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDH 122
           ESPKRSSPPSSG SWCIASQ+AS+K LQIALDY CGYGGTDCSAIQAGQRCYNPNTIHDH
Sbjct: 114 ESPKRSSPPSSGASWCIASQSASQKALQIALDYACGYGGTDCSAIQAGQRCYNPNTIHDH 173

Query: 123 ASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFG 182
           ASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSSVLNTT+SK STVFG
Sbjct: 174 ASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSVLNTTNSKGSTVFG 233

Query: 183 AVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           AVPSSP+PSAATCQEI VLQQLL LTIVSL LLHTNYL+G
Sbjct: 234 AVPSSPSPSAATCQEIKVLQQLLSLTIVSLTLLHTNYLHG 273

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BLAST of CsaV3_7G023130 vs. NCBI nr
Match: XP_038887351.1 (glucan endo-1,3-beta-glucosidase 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 2.4e-97
Identity = 194/220 (88.18%), Postives = 206/220 (93.64%), Query Frame = 0


Query: 3   GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAM 62
           GHTRELNHENQKFSS PV T+QRDITTPITTVPTIILSNPT STPFINPTST+DTYS  M
Sbjct: 53  GHTRELNHENQKFSS-PVSTTQRDITTPITTVPTIILSNPTASTPFINPTSTADTYSPVM 112

Query: 63  ESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDH 122
            SPKRSSPPSSG SWCIASQ+AS+K LQIALDY CGYGGTDCSAIQAGQ CYNPNTIHDH
Sbjct: 113 GSPKRSSPPSSGASWCIASQSASQKALQIALDYACGYGGTDCSAIQAGQSCYNPNTIHDH 172

Query: 123 ASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFG 182
           ASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSS+LNTT+SK STVFG
Sbjct: 173 ASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSILNTTNSKGSTVFG 232

Query: 183 AVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           AVPSSP+PSAATCQEINVL QLLLLT+VSL LLH+N L+G
Sbjct: 233 AVPSSPSPSAATCQEINVL-QLLLLTMVSLILLHSNNLHG 270

[Close]

BLAST of CsaV3_7G023130 vs. NCBI nr
Match: XP_038887352.1 (major pollen allergen Ole e 10-like isoform X2 [Benincasa hispida] >XP_038887353.1 major pollen allergen Ole e 10-like isoform X2 [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 2.4e-97
Identity = 194/220 (88.18%), Postives = 206/220 (93.64%), Query Frame = 0


Query: 3   GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAM 62
           GHTRELNHENQKFSS PV T+QRDITTPITTVPTIILSNPT STPFINPTST+DTYS  M
Sbjct: 19  GHTRELNHENQKFSS-PVSTTQRDITTPITTVPTIILSNPTASTPFINPTSTADTYSPVM 78

Query: 63  ESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDH 122
            SPKRSSPPSSG SWCIASQ+AS+K LQIALDY CGYGGTDCSAIQAGQ CYNPNTIHDH
Sbjct: 79  GSPKRSSPPSSGASWCIASQSASQKALQIALDYACGYGGTDCSAIQAGQSCYNPNTIHDH 138

Query: 123 ASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFG 182
           ASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSS+LNTT+SK STVFG
Sbjct: 139 ASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSILNTTNSKGSTVFG 198

Query: 183 AVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           AVPSSP+PSAATCQEINVL QLLLLT+VSL LLH+N L+G
Sbjct: 199 AVPSSPSPSAATCQEINVL-QLLLLTMVSLILLHSNNLHG 236

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BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 6.1e-19
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0


Query: 72  SSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYY 131
           ++  ++CIA +   RK+LQ ALD+ CG G  DCSA+  G+ CY P+ +  H++YAFN+YY
Sbjct: 355 TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYY 414

Query: 132 QK-NPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVL-NTTS----SKESTVFGAVP 191
           QK      SC+F G A +T+TDPS   C +  ++ S+  L N TS    S  ST  G +P
Sbjct: 415 QKMGKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTSGCIP 474

Query: 192 SSPTPSAATCQEINVLQQLLLLTIVSL 213
                  A+  ++ +L  LL++ +V L
Sbjct: 475 KYYHHPHASFGDLTLLSLLLIIALVFL 501

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.6e-17
Identity = 50/120 (41.67%), Postives = 62/120 (51.67%), Query Frame = 0


Query: 44  PSTPFINPTSTSDTYSSAMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTD 103
           P  P   PT +  T SS +  P    P      WC+    A+   LQ  +DY C   G D
Sbjct: 8   PDQPVPTPTPSVPTSSSPVPKP----PTQGNKKWCVPKAEATDAQLQSNIDYVCSQSGMD 67

Query: 104 CSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPVPD-SCNFGGTAVITSTDPSTMACEYTS 163
           C  IQA   C+NPNT+  HASYA NS+YQ     D  C+F GT  ITS+DPS  +C + S
Sbjct: 68  CGPIQANGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNGSCSFLS 123

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 7.5e-17
Identity = 56/139 (40.29%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 73  SGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQ 132
           S  SWCIAS  AS + L+ ALD+ CG G  DC+AIQ  Q C+ P+T+  HAS+ FNSY+Q
Sbjct: 365 SSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQ 424

Query: 133 KNPVPD-SCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFGAVPSSPTPS 192
           +N   D +C+FGG  V  + DPS   C Y +        N T +  +T         T S
Sbjct: 425 QNRATDVACSFGGAGVKVNKDPSYDKCIYITAGG-----NKTKATNATAL-------TSS 484

Query: 193 AATCQEINVLQQLLLLTIV 211
           A+T +   +LQ +L L ++
Sbjct: 485 ASTPRGNELLQWILKLCLM 491

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BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.9e-16
Identity = 52/123 (42.28%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 42  PTPS-TPFINPTSTSDTYSSAMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYG 101
           PTPS  P  NP            SPK +  PS G  WC+A   A+   LQ  ++Y CG+ 
Sbjct: 355 PTPSPNPSPNP------------SPKPA--PSGGGKWCVAKDGANGTDLQNNINYACGF- 414

Query: 102 GTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPVPD-SCNFGGTAVITSTDPSTMACE 161
             DC  IQ+G  C++PN++  HASY  N+YYQ N   D +C+F GT ++TS+DPS   C+
Sbjct: 415 -VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCK 461

Query: 162 YTS 163
           Y S
Sbjct: 475 YVS 461

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BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-15
Identity = 52/137 (37.96%), Postives = 74/137 (54.01%), Query Frame = 0


Query: 72  SSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYY 131
           ++  ++CIA      K LQ ALD+ CG G ++CS IQ G+ CY PN +  HAS+AFNSYY
Sbjct: 376 TTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYY 435

Query: 132 QK-NPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFGAVPSSPTP 191
           QK      SC+F G A+IT+TDPS  +C +  +    +   T  +      G   +S + 
Sbjct: 436 QKEGRASGSCDFKGVAMITTTDPSHGSCIFPGSKKVGNRTQTVVNSTEVAAGEA-TSRSL 495

Query: 192 SAATCQEINVLQQLLLL 208
           S   C  I +L    +L
Sbjct: 496 SRGFCVTIMILVTFSIL 511

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BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL
Match: A0A0A0K3M5 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G251640 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.4e-112
Identity = 214/222 (96.40%), Postives = 217/222 (97.75%), Query Frame = 0


Query: 1   MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSS 60
           MTGHTRELNHENQKFSSRPVF SQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYS 
Sbjct: 1   MTGHTRELNHENQKFSSRPVFISQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSP 60

Query: 61  AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH 120
           AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH
Sbjct: 61  AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH 120

Query: 121 DHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTV 180
           DHASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTT+SK STV
Sbjct: 121 DHASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTNSKGSTV 180

Query: 181 FGAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           FGAVPSSPTPSAATCQEINVLQ+LLLLTIVSLRL H NYLYG
Sbjct: 181 FGAVPSSPTPSAATCQEINVLQRLLLLTIVSLRLFHINYLYG 222

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL
Match: A0A0A0K8Y4 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G235750 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.6e-110
Identity = 210/221 (95.02%), Postives = 215/221 (97.29%), Query Frame = 0


Query: 2   TGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSA 61
           +GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYS A
Sbjct: 34  SGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSPA 93

Query: 62  MESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHD 121
           MESPKRSSPPSSG SWCIASQNASRKVLQIALDY CGYGGTDCSAIQAGQRCYNPNTIHD
Sbjct: 94  MESPKRSSPPSSGASWCIASQNASRKVLQIALDYACGYGGTDCSAIQAGQRCYNPNTIHD 153

Query: 122 HASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVF 181
           HASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSSVLNTT+SK STVF
Sbjct: 154 HASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSVLNTTNSKGSTVF 213

Query: 182 GAVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           GAVPSSPTPSAATCQEINVLQ+LLLLTIVSLRL H NYLYG
Sbjct: 214 GAVPSSPTPSAATCQEINVLQRLLLLTIVSLRLFHINYLYG 254

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL
Match: A0A1S4DWJ2 (glucan endo-1,3-beta-glucosidase 3-like OS=Cucumis melo OX=3656 GN=LOC103489662 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.6e-105
Identity = 202/220 (91.82%), Postives = 210/220 (95.45%), Query Frame = 0


Query: 3   GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAM 62
           GH REL+HENQKFSSRPVFTSQRDITTPITTVPTIILSNPT STPFINPTSTSDTYS AM
Sbjct: 54  GHARELSHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTASTPFINPTSTSDTYSPAM 113

Query: 63  ESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDH 122
           ESPKRSSPPSSG SWCIASQ+AS+K LQIALDY CGYGGTDCSAIQAGQRCYNPNTIHDH
Sbjct: 114 ESPKRSSPPSSGASWCIASQSASQKALQIALDYACGYGGTDCSAIQAGQRCYNPNTIHDH 173

Query: 123 ASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFG 182
           ASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSSVLNTT+SK STVFG
Sbjct: 174 ASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSVLNTTNSKGSTVFG 233

Query: 183 AVPSSPTPSAATCQEINVLQQLLLLTIVSLRLLHTNYLYG 223
           AVPSSP+PSAATCQEI VLQQLL LTIVSL LLHTNYL+G
Sbjct: 234 AVPSSPSPSAATCQEIKVLQQLLSLTIVSLTLLHTNYLHG 273

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL
Match: A0A5A7SSE1 (Glucan endo-1,3-beta-glucosidase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00020 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 8.4e-96
Identity = 184/198 (92.93%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 1   MTGHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSS 60
           MTGH REL+HENQKFSSRPVFTSQRDITTPITTVPTIILSNPT STPFINPTSTSDTYS 
Sbjct: 1   MTGHARELSHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTASTPFINPTSTSDTYSP 60

Query: 61  AMESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIH 120
           AMESPKRSSPPSSG SWCIASQ+AS+K LQIALDY CGYGGTDCSAIQAGQRCYNPNTIH
Sbjct: 61  AMESPKRSSPPSSGASWCIASQSASQKALQIALDYACGYGGTDCSAIQAGQRCYNPNTIH 120

Query: 121 DHASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTV 180
           DHASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+YTSTSTSSSVLNTT+SK STV
Sbjct: 121 DHASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYTSTSTSSSVLNTTNSKGSTV 180

Query: 181 FGAVPSSPTPSAATCQEI 199
           FGAVPSSP+PSAATCQEI
Sbjct: 181 FGAVPSSPSPSAATCQEI 198

[Close]

BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL
Match: A0A6J1D2V3 (glucan endo-1,3-beta-glucosidase 12-like OS=Momordica charantia OX=3673 GN=LOC111016792 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.4e-89
Identity = 174/207 (84.06%), Postives = 190/207 (91.79%), Query Frame = 0


Query: 3   GHTRELNHENQKFSSRPVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAM 62
           GHTRELNHE +KF+S PV T+QRDITTPITTVPTIILSNPT STP INPTSTSD+YS AM
Sbjct: 71  GHTRELNHEKKKFNS-PVSTTQRDITTPITTVPTIILSNPTASTPLINPTSTSDSYSPAM 130

Query: 63  ESPKRSSPPSSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDH 122
           E+PK+SSPPSSG SWCIASQ+ S+  LQ+A+DY CGYGG DCSAIQ GQ CYNPNTIHDH
Sbjct: 131 ETPKKSSPPSSGGSWCIASQSVSQTALQVAIDYACGYGGADCSAIQPGQSCYNPNTIHDH 190

Query: 123 ASYAFNSYYQKNPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFG 182
           ASYAFNSYYQKNPVP+SCNFGGTAVITSTDPSTMAC+Y STSTSSS+LNTT+SK STVFG
Sbjct: 191 ASYAFNSYYQKNPVPNSCNFGGTAVITSTDPSTMACQYASTSTSSSILNTTNSKGSTVFG 250

Query: 183 AVPSSPTPSAATCQEINVLQQLLLLTI 210
           AVPSSP+PSAA CQEINVLQQLLLLT+
Sbjct: 251 AVPSSPSPSAAHCQEINVLQQLLLLTV 276

[Close]

BLAST of CsaV3_7G023130 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-37
Identity = 88/174 (50.57%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 24  QRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAMESPKRSSPPS-------SGTS 83
           ++DITTP+ T PT      TP+T  + P S SD  +SA+ +   + P S          S
Sbjct: 31  EKDITTPLATNPT-----TTPTT--VVPNSDSD--ASAVATTPLTIPSSPHGVAYPGDQS 90

Query: 84  WCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPV 143
           WC+A +N ++  LQ ALDY CG GG DCS IQ G  CYNPN++  HAS+AFNSYYQKNP+
Sbjct: 91  WCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPI 150

Query: 144 PDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFGAVPSSPTP 191
           P SCNF GTA+  S DPS  +C + STSTS S+LN TS     +FG +PS PTP
Sbjct: 151 PSSCNFDGTAITISADPSLGSCHFPSTSTSESILNVTSEDGLGLFGRIPSHPTP 195

[Close]

BLAST of CsaV3_7G023130 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 7.9e-30
Identity = 67/118 (56.78%), Postives = 78/118 (66.10%), Query Frame = 0


Query: 77  WCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPV 136
           WCIA  NAS   LQ+ALDY CGYGG DC  IQ G  CY PNTI DHAS+AFNSYYQK+P 
Sbjct: 148 WCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKHPG 207

Query: 137 PDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVLNTTSSKESTVFGAVPSSPTPSAAT 195
            DSCNFGG A +TSTDPS  +C ++S+S + S  +  S      F + PSS  P   T
Sbjct: 208 SDSCNFGGAAQLTSTDPSKGSCHFSSSSGTVST-SPPSQMSPPDFSSPPSSTYPPPIT 264

[Close]

BLAST of CsaV3_7G023130 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 6.7e-29
Identity = 70/138 (50.72%), Postives = 88/138 (63.77%), Query Frame = 0


Query: 24  QRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAMESPKRSSPPS-------SGTS 83
           ++DITTP+ T PT      TP+T  + P S SD  +SA+ +   + P S          S
Sbjct: 31  EKDITTPLATNPT-----TTPTT--VVPNSDSD--ASAVATTPLTIPSSPHGVAYPGDQS 90

Query: 84  WCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPV 143
           WC+A +N ++  LQ ALDY CG GG DCS IQ G  CYNPN++  HAS+AFNSYYQKNP+
Sbjct: 91  WCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPI 150

Query: 144 PDSCNFGGTAVITSTDPS 155
           P SCNF GTA+  S DPS
Sbjct: 151 PSSCNFDGTAITISADPS 159

[Close]

BLAST of CsaV3_7G023130 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 7.7e-25
Identity = 76/180 (42.22%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 19  PVFTSQRDITTPITTVPTIILSNPTPSTPFINPTSTSDTYSSAMESPKRSSPPS-SGTSW 78
           PV T      TP  T P   ++NP    P   P+ T       +  P  S+ PS SG SW
Sbjct: 81  PVITIPPPTLTPPVTNP---VTNPVTQYPPTQPSGTVPVPVPVVAPPVVSNSPSVSGQSW 140

Query: 79  CIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYYQKNPVP 138
           C+A   AS+  LQ ALDY CG    DCS +Q G  CY+P ++  HAS+AFNSYYQKNP P
Sbjct: 141 CVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSP 200

Query: 139 DSCNFGGTAVITSTDPSTMACEY---TSTSTSSSVLNTTSSKESTVFGAVPSSPTPSAAT 195
            SC+FGG A + +T+PST +C Y   +STST  +   TT +  +      P + TP   T
Sbjct: 201 QSCDFGGAASLVNTNPSTGSCIYQTGSSTSTPMTAGTTTPTPSTQTVNQPPVTSTPIIPT 255

[Close]

BLAST of CsaV3_7G023130 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 95.9 bits (237), Expect = 4.4e-20
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0


Query: 72  SSGTSWCIASQNASRKVLQIALDYTCGYGGTDCSAIQAGQRCYNPNTIHDHASYAFNSYY 131
           ++  ++CIA +   RK+LQ ALD+ CG G  DCSA+  G+ CY P+ +  H++YAFN+YY
Sbjct: 355 TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYY 414

Query: 132 QK-NPVPDSCNFGGTAVITSTDPSTMACEYTSTSTSSSVL-NTTS----SKESTVFGAVP 191
           QK      SC+F G A +T+TDPS   C +  ++ S+  L N TS    S  ST  G +P
Sbjct: 415 QKMGKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTSGCIP 474

Query: 192 SSPTPSAATCQEINVLQQLLLLTIVSL 213
                  A+  ++ +L  LL++ +V L
Sbjct: 475 KYYHHPHASFGDLTLLSLLLIIALVFL 501

The following BLAST results are available for this feature:

BLAST of CsaV3_7G023130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148305.3	3.1e-116	100.00	major pollen allergen Ole e 10 [Cucumis sativus] >KAE8646120.1 hypothetical prot...	[more]
XP_031744909.1	5.8e-107	93.21	glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus] >KAE8646097.1 h...	[more]
XP_016900361.1	1.6e-104	91.82	PREDICTED: glucan endo-1,3-beta-glucosidase 3-like [Cucumis melo] >XP_016900362....	[more]
XP_038887351.1	2.4e-97	88.18	glucan endo-1,3-beta-glucosidase 3-like isoform X1 [Benincasa hispida]	[more]
XP_038887352.1	2.4e-97	88.18	major pollen allergen Ole e 10-like isoform X2 [Benincasa hispida] >XP_038887353...	[more]

BLAST of CsaV3_7G023130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZU91	6.1e-19	38.78	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q84V39	2.6e-17	41.67	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9FJU9	7.5e-17	40.29	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
P52409	2.9e-16	42.28	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
O65399	1.1e-15	37.96	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]

BLAST of CsaV3_7G023130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3M5	6.4e-112	96.40	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G251640 PE=4 SV=...	[more]
A0A0A0K8Y4	4.6e-110	95.02	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G235750 PE=4 SV=...	[more]
A0A1S4DWJ2	7.6e-105	91.82	glucan endo-1,3-beta-glucosidase 3-like OS=Cucumis melo OX=3656 GN=LOC103489662 ...	[more]
A0A5A7SSE1	8.4e-96	92.93	Glucan endo-1,3-beta-glucosidase 3-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1D2V3	3.4e-89	84.06	glucan endo-1,3-beta-glucosidase 12-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of CsaV3_7G023130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30933.1	3.0e-37	50.57	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	7.9e-30	56.78	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	6.7e-29	50.72	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	7.7e-25	42.22	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G01630.1	4.4e-20	38.78	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 76..160 e-value: 4.6E-42 score: 155.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 77..146 e-value: 7.7E-20 score: 71.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 72..183 e-value: 3.0E-11 score: 45.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..76
None	No IPR available	PANTHER	PTHR32227:SF338	BETA 1,3-GLUCANASE-LIKE	coord: 7..183
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 7..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_7G023130.1	CsaV3_7G023130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds