CsaV3_2G012080 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G012080
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic
Locationchr2: 9534360 .. 9536321 (+)
RNA-Seq ExpressionCsaV3_2G012080
SyntenyCsaV3_2G012080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTTTCCCTCTTCTTCTTCATACAATTTTACAATTATAGTCATGCAAATCAGTTTACAATCGCCACCCCCGCCGCTGACACTTTGCCGGAGCTCCTCCACCATCGAAACCCCTCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACGCCCACCACTGTCCCTCCCAAAAAAGGTGTCGCCGCCCCAGCGTCGGCTTAACCCATTACAGAAATTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTTGATAAATTGCCGAAAACGATTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCCGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCATTTATTTACGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGTGGTAACAAAGCTAGCTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGCAGGAAGCCGCTTTAGGCCGACTTGTTTTCCCTAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTTGCAATCTTTTTGGCTCGAGCCGGGATCGGGTTAATCGACGGCTCGAAAGGGACAGGTGTTGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTTTATCTGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCAACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCTATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTAAGGTTCGACCGGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAACCCACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCACCAAGTGGTTTTCAAGCTATTAGAAATGGTTCGGGGTGGATCACCCGTGGTGTTCGACCCGAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAAGGAATTGTTTGGATTGAGGTGCCGAATTGCTTTTGCTTCCACTTGTGGAATGCATGGGAGGAAACAGGTGGTAATGACGAGAAAAGTATCGTTGTGGTCGGGTCGTGTATGAACCCGCCTGATTCCATTTTCAACGACCGGGACCAACCATTAAGAATCGAGCTGACGGAAATCCGGATGGATGTGAAGAGCCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAGGGTTGGTTGGGCGGAAAACTCGGTTCGTTTATATGGCCATAGCGGACCCTTGGCCCAAATGTTCCGGCATTGCGAAGGTGGATTTGGAAACTGGGAAGGTGAAGAAGTTTCTATACGGTGAGGGCCGATATGGCGGGGAGCCGTTTTACGTGCCGGAAAATGGGAATTGGGGGGGAGATAATGAGAAGGAGGACGGAGGGTATATTGTAGGGTTTGTGAGGGATGAGAAGCGGGAGAGGTCGGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCAGTGAGGTTGCCAGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGTAATTAAAAATTATTTTGGGGCTTTATGTGGTTGTTTTTTGTGTAAATTAATGGCTTTGTAGATTATTTTGATTTTATTTATTTTCTATGGTGGTGGTTAGGTTTGTGATAATTAGTGGTTTTTAAAGATGTAATTAATGTAGATATAACTTATGTAATATCTAATTAAGGAATAAAATTGAGCAAACATATATTTGACGTATACAAACAAATTGGAA

mRNA sequence

ATGCAAATCAGTTTACAATCGCCACCCCCGCCGCTGACACTTTGCCGGAGCTCCTCCACCATCGAAACCCCTCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACGCCCACCACTGTCCCTCCCAAAAAAGGTGTCGCCGCCCCAGCGTCGGCTTAACCCATTACAGAAATTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTTGATAAATTGCCGAAAACGATTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCCGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCATTTATTTACGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGTGGTAACAAAGCTAGCTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGCAGGAAGCCGCTTTAGGCCGACTTGTTTTCCCTAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTTGCAATCTTTTTGGCTCGAGCCGGGATCGGGTTAATCGACGGCTCGAAAGGGACAGGTGTTGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTTTATCTGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCAACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCTATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTAAGGTTCGACCGGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAACCCACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCACCAAGTGGTTTTCAAGCTATTAGAAATGGTTCGGGGTGGATCACCCGTGGTGTTCGACCCGAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAAGGAATTGTTTGGATTGAGGTGCCGAATTGCTTTTGCTTCCACTTGTGGAATGCATGGGAGGAAACAGGTGGTAATGACGAGAAAAGTATCGTTGTGGTCGGGTCGTGTATGAACCCGCCTGATTCCATTTTCAACGACCGGGACCAACCATTAAGAATCGAGCTGACGGAAATCCGGATGGATGTGAAGAGCCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAGGGTTGGTTGGGCGGAAAACTCGGTTCGTTTATATGGCCATAGCGGACCCTTGGCCCAAATGTTCCGGCATTGCGAAGGTGGATTTGGAAACTGGGAAGGTGAAGAAGTTTCTATACGGTGAGGGCCGATATGGCGGGGAGCCGTTTTACGTGCCGGAAAATGGGAATTGGGGGGGAGATAATGAGAAGGAGGACGGAGGGTATATTGTAGGGTTTGTGAGGGATGAGAAGCGGGAGAGGTCGGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCAGTGAGGTTGCCAGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGTAATTAA

Coding sequence (CDS)

ATGCAAATCAGTTTACAATCGCCACCCCCGCCGCTGACACTTTGCCGGAGCTCCTCCACCATCGAAACCCCTCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACGCCCACCACTGTCCCTCCCAAAAAAGGTGTCGCCGCCCCAGCGTCGGCTTAACCCATTACAGAAATTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTTGATAAATTGCCGAAAACGATTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCCGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCATTTATTTACGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGTGGTAACAAAGCTAGCTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGCAGGAAGCCGCTTTAGGCCGACTTGTTTTCCCTAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTTGCAATCTTTTTGGCTCGAGCCGGGATCGGGTTAATCGACGGCTCGAAAGGGACAGGTGTTGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTTTATCTGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCAACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCTATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTAAGGTTCGACCGGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAACCCACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCACCAAGTGGTTTTCAAGCTATTAGAAATGGTTCGGGGTGGATCACCCGTGGTGTTCGACCCGAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAAGGAATTGTTTGGATTGAGGTGCCGAATTGCTTTTGCTTCCACTTGTGGAATGCATGGGAGGAAACAGGTGGTAATGACGAGAAAAGTATCGTTGTGGTCGGGTCGTGTATGAACCCGCCTGATTCCATTTTCAACGACCGGGACCAACCATTAAGAATCGAGCTGACGGAAATCCGGATGGATGTGAAGAGCCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAGGGTTGGTTGGGCGGAAAACTCGGTTCGTTTATATGGCCATAGCGGACCCTTGGCCCAAATGTTCCGGCATTGCGAAGGTGGATTTGGAAACTGGGAAGGTGAAGAAGTTTCTATACGGTGAGGGCCGATATGGCGGGGAGCCGTTTTACGTGCCGGAAAATGGGAATTGGGGGGGAGATAATGAGAAGGAGGACGGAGGGTATATTGTAGGGTTTGTGAGGGATGAGAAGCGGGAGAGGTCGGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCAGTGAGGTTGCCAGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGTAATTAA

Protein sequence

MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQARN*
Homology
BLAST of CsaV3_2G012080 vs. NCBI nr
Match: XP_004150345.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis sativus] >KGN61528.1 hypothetical protein Csa_006609 [Cucumis sativus])

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA
Sbjct: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of CsaV3_2G012080 vs. NCBI nr
Match: XP_008460725.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis melo] >KAA0032007.1 9-cis-epoxycarotenoid dioxygenase NCED6 [Cucumis melo var. makuwa] >TYK16828.1 9-cis-epoxycarotenoid dioxygenase NCED6 [Cucumis melo var. makuwa])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 543/568 (95.60%), Postives = 552/568 (97.18%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQISL SP PPLT CRSSSTIETPPIRKLKVITPP PPLSLPKKVSPP R+LNPLQ FAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+YLRNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQMNR  VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of CsaV3_2G012080 vs. NCBI nr
Match: XP_038875554.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 522/568 (91.90%), Postives = 540/568 (95.07%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQI LQSPPP  +LCRSSSTIETPPIRKLKVITPP PPLSLPK VSPP RRLNP Q  AA
Sbjct: 1   MQIYLQSPPPLPSLCRSSSTIETPPIRKLKVITPPPPPLSLPKTVSPPLRRLNPFQNLAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSL VDKLPKT+DP VQISGNFSPVPEC VRHDLEIIGNLPACLRG+YLRNGA
Sbjct: 61  SLLDAVESSLIVDKLPKTVDPTVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAP GGHHLFDGDGMIHAVTFH GNKASYSCRFTRT+RLQQEAALGR VFPKPIGEL
Sbjct: 121 NPMHAPIGGHHLFDGDGMIHAVTFHCGNKASYSCRFTRTSRLQQEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGLIDGSKGTGV NAGLVYFNGRLLALSEDDLPYHV+IKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVTNAGLVYFNGRLLALSEDDLPYHVKIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TL EPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP K SRFG+LPKS VDEK
Sbjct: 301 TLPEPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKISRFGVLPKSEVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVW+EVPNCFCFHLWNAWEETG NDEK++VV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWMEVPNCFCFHLWNAWEETGDNDEKTVVVLGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQ+NRGLVGR+TRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQVNRGLVGRRTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYG+GRYGGEPFYV ENGN GGDNE EDGG++VGFVRDEKRERSE+VVVKAAEMEEVA
Sbjct: 481 KFLYGDGRYGGEPFYVSENGNCGGDNENEDGGFVVGFVRDEKRERSELVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 AVRLPARVPYGFHGTFVSEEELKGQAPN 568

BLAST of CsaV3_2G012080 vs. NCBI nr
Match: KAG6574873.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013444.1 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 496/570 (87.02%), Postives = 526/570 (92.28%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVIT--PPRPPLSLPKKV--SPPQRRLNPLQ 60
           MQISLQSP P   LCRSSST+ET PIRKLK++T  PP PPL  P KV  SP  RRLNP Q
Sbjct: 1   MQISLQSPLP---LCRSSSTVETTPIRKLKLLTLPPPSPPLPPPPKVVLSPLPRRLNPFQ 60

Query: 61  KFAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYL 120
             AASLLDAVESSL VDKLPKT+DPAVQISGNFSPVPEC VRHDLEIIGNLPACLRG+YL
Sbjct: 61  NLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYL 120

Query: 121 RNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKP 180
           RNGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+QE ALGR VFPKP
Sbjct: 121 RNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPKP 180

Query: 181 IGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIK 240
           IGELHGHQGLARLAIFLARAGIGLID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I+
Sbjct: 181 IGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIR 240

Query: 241 DDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSR 300
           D GDLETIGRF+FN QI+CPMIAHPKVDP+SGDLHGLSYNMIKKPYLKYLRFDR GKK R
Sbjct: 241 DGGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRFDRLGKKVR 300

Query: 301 DVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSG 360
           DVDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKSG
Sbjct: 301 DVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSG 360

Query: 361 VDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELT 420
           VDE GIVWI+VP+CFCFHLWNAWEETG N EK++V VGSCMNPPDSIFNDRDQ L IELT
Sbjct: 361 VDETGIVWIDVPDCFCFHLWNAWEETGTNGEKTVVAVGSCMNPPDSIFNDRDQALTIELT 420

Query: 421 EIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLET 480
           EIRMD+++RKVTRRVFGSGMNLEAGQ+NR LVGR+TRFVYMAIA+PWPKCSGIAKVDLET
Sbjct: 421 EIRMDMRNRKVTRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLET 480

Query: 481 GKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEM 540
           GKVKKFLYG+GRYGGEPFYV ENGN  G++E EDGGY+VGFVRDEKRERSE+VVVKAAEM
Sbjct: 481 GKVKKFLYGDGRYGGEPFYVSENGN-SGEDENEDGGYLVGFVRDEKRERSELVVVKAAEM 540

Query: 541 EEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           +EVAAVRLP RVPYGFHGTFVSEEEL GQA
Sbjct: 541 KEVAAVRLPARVPYGFHGTFVSEEELKGQA 566

BLAST of CsaV3_2G012080 vs. NCBI nr
Match: XP_023548938.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.3e-291
Identity = 496/573 (86.56%), Postives = 526/573 (91.80%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVIT--PPRPPLSLPKK---VSPPQRRLNPL 60
           MQISLQSP P   LCRSSST+ETPPIRKLK++T  PP PP S P     +SP  RRLNP 
Sbjct: 1   MQISLQSPLP---LCRSSSTVETPPIRKLKLLTLPPPSPPPSPPPPKVVLSPLPRRLNPF 60

Query: 61  QKFAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIY 120
           Q  AASLLDAVESSL VDKLPKT+DPAVQISGNFSPVPEC VRHDLEIIGNLPACLRG+Y
Sbjct: 61  QNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVY 120

Query: 121 LRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPK 180
           LRNGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+QE ALGR VFPK
Sbjct: 121 LRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPK 180

Query: 181 PIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQI 240
           PIGELHGHQGLARLAIFLARAGIGLID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I
Sbjct: 181 PIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKI 240

Query: 241 KDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKS 300
           +D GDLETIGRF+FN QI+CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDR GKK 
Sbjct: 241 RDGGDLETIGRFSFNSQISCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRLGKKV 300

Query: 301 RDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKS 360
           RDVDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKS
Sbjct: 301 RDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKS 360

Query: 361 GVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIEL 420
           GVDE GIVWI+VP+CFCFHLWNAWEETG N EK++V VGSCMNPPDSIFNDRDQ L IEL
Sbjct: 361 GVDETGIVWIDVPDCFCFHLWNAWEETGINGEKTVVAVGSCMNPPDSIFNDRDQALTIEL 420

Query: 421 TEIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLE 480
           TEIRMD+++RK TRRVFGSGMNLEAGQ+NR LVGR+TRFVYMAIA+PWPKCSGIAKVDLE
Sbjct: 421 TEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLE 480

Query: 481 TGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAE 540
           TGKVKKFLYG+GRYGGEPFYV ENGN  G++E EDGGY+VGFVRDEKRERSE+VVVKAAE
Sbjct: 481 TGKVKKFLYGDGRYGGEPFYVSENGN-SGEDENEDGGYVVGFVRDEKRERSELVVVKAAE 540

Query: 541 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQARN 569
           M+EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 MKEVAAVRLPARVPYGFHGTFVSEEELKGQAPN 569

BLAST of CsaV3_2G012080 vs. ExPASy Swiss-Prot
Match: Q9LRM7 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED6 PE=2 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 8.7e-203
Identity = 346/581 (59.55%), Postives = 440/581 (75.73%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPP---------IRKLKVITPP--RPPLSLPKKVSPPQ 60
           MQ SL+S   P      S  +  P          I   K+ T P    P+  P K+ P  
Sbjct: 1   MQHSLRSDLLPTKTSPRSHLLPQPKNANISRRILINPFKIPTLPDLTSPVPSPVKLKPTY 60

Query: 61  RRLNPLQKFAASLLDAVESSLFVD-----KLPKTIDPAVQISGNFSPVPECSVRHDLEII 120
             LN LQK AA++LD +ESS+ +       LPK  DPAVQ+SGNF+PV EC V++ LE++
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 121 GNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQ 180
           G +P+CL+G+Y+RNGANPM  P  GHHLFDGDGMIHAV+    N+ SYSCR+T+TNRL Q
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQ 180

Query: 181 EAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLAL 240
           E ALGR VFPKPIGELHGH GLARLA+F ARAGIGL+DG++G GVANAG+V+FNGRLLA+
Sbjct: 181 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAM 240

Query: 241 SEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLK 300
           SEDDLPY V+I   GDLETIGRF F+ QI+  +IAHPKVD  +GDLH LSYN++KKP+L+
Sbjct: 241 SEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLR 300

Query: 301 YLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPK 360
           YL+F+  GKK+RDV+ITL EPTMIHDFAITEN VVIPD Q+VFKL EM+RGGSPV++  +
Sbjct: 301 YLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKE 360

Query: 361 KTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIF 420
           K +RFG+L K  +    I W++VP+CFCFHLWNAWEE     +  IVV+GSCM+PPD+IF
Sbjct: 361 KMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIF 420

Query: 421 NDRDQPLRIELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWP 480
           ++  +P R+EL+EIR+++++++  R+V  +G+NLEAG +NR  VGRK++FVY+AIADPWP
Sbjct: 421 SESGEPTRVELSEIRLNMRTKESNRKVIVTGVNLEAGHINRSYVGRKSQFVYIAIADPWP 480

Query: 481 KCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRE 540
           KCSGIAKVD++ G V +F YG  R+GGEP +VPE     G+ E ED GY++GFVRDE+++
Sbjct: 481 KCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-----GEGE-EDKGYVMGFVRDEEKD 540

Query: 541 RSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 566
            SE VVV A +M++VAAVRLP RVPYGFHGTFVSE +L  Q
Sbjct: 541 ESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

BLAST of CsaV3_2G012080 vs. ExPASy Swiss-Prot
Match: Q5MBR5 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED3 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 6.3e-185
Identity = 313/526 (59.51%), Postives = 385/526 (73.19%), Query Frame = 0

Query: 50  RRLNPLQKFAASLLDAVESSLFVD------KLPKTIDPAVQISGNFSPVPECSVRHDLEI 109
           +RLN  Q+ AA  LDA E     +       LP T DPAVQI+GNF+PV E      L +
Sbjct: 87  KRLNFFQRAAAMALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGETPPARALPV 146

Query: 110 IGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQ 169
            G +P  + G+Y RNGANP   P  GHHLFDGDGM+HAV   +G   SY+CRFT T RL+
Sbjct: 147 SGRIPPFINGVYARNGANPHFDPVAGHHLFDGDGMVHAVRIRNGAAESYACRFTETARLR 206

Query: 170 QEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLA 229
           QE A+GR +FPK IGELHGH G+ARLA+F ARA  GL+D S GTGVANAGL+YFNGRLLA
Sbjct: 207 QERAMGRPMFPKAIGELHGHSGIARLALFYARAACGLLDPSHGTGVANAGLIYFNGRLLA 266

Query: 230 LSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYL 289
           +SEDDLPY V++  DGDLET+GR++F+GQ+ C MIAHPK+DP +G+LH LSY++IKKPYL
Sbjct: 267 MSEDDLPYQVRVTADGDLETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKKPYL 326

Query: 290 KYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDP 349
           KY  F   G KS DV+I L +PTMIHDFAITEN+VV+PDHQVVFKL EM+RGGSPVV D 
Sbjct: 327 KYFYFAPDGTKSADVEIPLDQPTMIHDFAITENYVVVPDHQVVFKLQEMLRGGSPVVLDK 386

Query: 350 KKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSI 409
           +KTSRFG+LPK   D   +VW++VP+CFCFHLWNAWEE    D   +VV+GSCM P DSI
Sbjct: 387 EKTSRFGVLPKHAADASEMVWVDVPDCFCFHLWNAWEEA---DTDEVVVIGSCMTPADSI 446

Query: 410 FNDRDQPLRIELTEIRMDVKSRKVTRRVF---GSGMNLEAGQMNRGLVGRKTRFVYMAIA 469
           FN+ D  L   LTEIR++ ++ + TRR      S +NLE G +NR L+GRKTR+ Y+A+A
Sbjct: 447 FNESDDRLESVLTEIRLNTRTGESTRRAILPPSSQVNLEVGMVNRNLLGRKTRYAYLAVA 506

Query: 470 DPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRD 529
           +PWPK SG AKVDL TG++ KF YGEGR+GGEP +VP +         ED GYI+ FV D
Sbjct: 507 EPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDAA-AATPRGEDDGYILSFVHD 566

Query: 530 EKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           E+   SE++VV AA+M   A V+LP RVPYGFHGTF++ +EL  QA
Sbjct: 567 ERAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFITGDELTTQA 608

BLAST of CsaV3_2G012080 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 6.3e-185
Identity = 316/555 (56.94%), Postives = 410/555 (73.87%), Query Frame = 0

Query: 18  SSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASLLDAVESSLFVDKL-- 77
           SS++++P  +            +L  K +    +LNP+Q+ AA +LDAVE+++   +   
Sbjct: 111 SSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRR 170

Query: 78  ---PKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHL 137
              PKT DPAVQI+GNF PVPE  V H+L + G +P C++G+Y+RNGANP+H P  GHHL
Sbjct: 171 HPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHL 230

Query: 138 FDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIF 197
           FDGDGM+HAV F +G+  SY+CRFT TNRL QE   GR VFPK IGELHGH G+A+L +F
Sbjct: 231 FDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLF 290

Query: 198 LARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQ 257
             R   GL+D + G GVANAGLVYFNG LLA+SEDDLPYHV++   GDLET GR++F+GQ
Sbjct: 291 NTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQ 350

Query: 258 INCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFA 317
           +   MIAHPK+DP + +L  LSY+++ KPYLKY RF   G+KS DV+I L +PTMIHDFA
Sbjct: 351 LKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFA 410

Query: 318 ITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFC 377
           ITEN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+  D   I WIEVP+CFC
Sbjct: 411 ITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFC 470

Query: 378 FHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR-V 437
           FHLWN+WEE    +   +VV+GSCM PPDSIFN+ D+ L+  L+EIR+++K+ + TRR V
Sbjct: 471 FHLWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPV 530

Query: 438 FGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGG 497
               +NLEAG +NR L+GRKTR+ Y+A+ +PWPK SG AKVDL TG+++K++YGEG+YGG
Sbjct: 531 ISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGG 590

Query: 498 EPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYG 557
           EP ++P      GD E EDGGYI+ FV DE++ +SE+ ++ A  M+  A V LP RVPYG
Sbjct: 591 EPLFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYG 650

Query: 558 FHGTFVSEEELNGQA 567
           FHGTF+S+E+L+ QA
Sbjct: 651 FHGTFISKEDLSKQA 655

BLAST of CsaV3_2G012080 vs. ExPASy Swiss-Prot
Match: O24592 (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 PE=1 SV=2)

HSP 1 Score: 648.3 bits (1671), Expect = 8.2e-185
Identity = 316/551 (57.35%), Postives = 398/551 (72.23%), Query Frame = 0

Query: 25  PIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASLLDAVESSLFVD------KLPKT 84
           P RK   I  P    + P+K    +++LN  Q+ AA+ LDA E     +       LP T
Sbjct: 58  PSRKPAAIAVP-GHAAAPRKAEGGKKQLNLFQRAAAAALDAFEEGFVANVLERPHGLPST 117

Query: 85  IDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGM 144
            DPAVQI+GNF+PV E    H+L + G +P  + G+Y RNGANP   P  GHHLFDGDGM
Sbjct: 118 ADPAVQIAGNFAPVGERPPVHELPVSGRIPPFIDGVYARNGANPCFDPVAGHHLFDGDGM 177

Query: 145 IHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGI 204
           +HA+   +G   SY+CRFT T RL+QE A+GR VFPK IGELHGH G+ARLA+F ARA  
Sbjct: 178 VHALRIRNGAAESYACRFTETARLRQERAIGRPVFPKAIGELHGHSGIARLALFYARAAC 237

Query: 205 GLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMI 264
           GL+D S GTGVANAGLVYFNGRLLA+SEDDLPYHV++ DDGDLET+GR++F+GQ+ C MI
Sbjct: 238 GLVDPSAGTGVANAGLVYFNGRLLAMSEDDLPYHVRVADDGDLETVGRYDFDGQLGCAMI 297

Query: 265 AHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHV 324
           AHPK+DP +G+LH LSY++IK+PYLKY  F   G KS DV+I L +PTMIHDFAITEN V
Sbjct: 298 AHPKLDPATGELHALSYDVIKRPYLKYFYFRPDGTKSDDVEIPLEQPTMIHDFAITENLV 357

Query: 325 VIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNA 384
           V+PDHQVVFKL EM+RGGSPVV D +KTSRFG+LPK   D   + W++VP+CFCFHLWNA
Sbjct: 358 VVPDHQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMAWVDVPDCFCFHLWNA 417

Query: 385 WEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVF---GSG 444
           WE+    +   +VV+GSCM P DSIFN+ D+ L   LTEIR+D ++ + TRR        
Sbjct: 418 WEDEATGE---VVVIGSCMTPADSIFNESDERLESVLTEIRLDARTGRSTRRAVLPPSQQ 477

Query: 445 MNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFY 504
           +NLE G +NR L+GR+TR+ Y+A+A+PWPK SG AKVDL TG++ KF YGEGR+GGEP +
Sbjct: 478 VNLEVGMVNRNLLGRETRYAYLAVAEPWPKVSGFAKVDLSTGELTKFEYGEGRFGGEPCF 537

Query: 505 VPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGT 564
           VP +         ED GY++ FV DE+   SE++VV AA+M   A V+LP RVP+GFHGT
Sbjct: 538 VPMDPA-AAHPRGEDDGYVLTFVHDERAGTSELLVVNAADMRLEATVQLPSRVPFGFHGT 597

Query: 565 FVSEEELNGQA 567
           F++ +EL  QA
Sbjct: 598 FITGQELEAQA 603

BLAST of CsaV3_2G012080 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 647.9 bits (1670), Expect = 1.1e-184
Identity = 326/571 (57.09%), Postives = 411/571 (71.98%), Query Frame = 0

Query: 4   SLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASLL 63
           SLQ+ PP L   + SS  +TP     K  T   P        S    + N +QK AA  L
Sbjct: 49  SLQA-PPILHFPKQSSNYQTP-----KNNTISHPKQENNNSSSSSTSKWNLVQKAAAMAL 108

Query: 64  DAVESSL----FVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNG 123
           DAVES+L        LPKT DP VQISGNF+PVPE  V   L + G +P C++G+Y+RNG
Sbjct: 109 DAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNG 168

Query: 124 ANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGE 183
           ANP+  PT GHH FDGDGM+HAV F +G+ ASY+CRFT T RL QE ALGR VFPK IGE
Sbjct: 169 ANPLFEPTAGHHFFDGDGMVHAVQFKNGS-ASYACRFTETERLVQEKALGRPVFPKAIGE 228

Query: 184 LHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDG 243
           LHGH G+ARL +F AR   GL+D SKGTGVANAGLVYFN RLLA+SEDDLPYHV++   G
Sbjct: 229 LHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTG 288

Query: 244 DLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVD 303
           DL+T GRF+F+GQ+   MIAHPK+DP+SG+L  LSY++I+KPYLKY RF + G+KS DV+
Sbjct: 289 DLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVE 348

Query: 304 ITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDE 363
           I + +PTM+HDFAITEN VVIPD QVVFK+ EM+RGGSPVV+D  K SRFGIL K   D 
Sbjct: 349 IPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDG 408

Query: 364 KGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIR 423
             + W+EVP+CFCFHLWNAWEE    +   IVV+GSCM PPDSIFN+ D+ L+  L+EIR
Sbjct: 409 SDLKWVEVPDCFCFHLWNAWEEA---ETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIR 468

Query: 424 MDVKSRKVTRRVF----GSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLE 483
           +++K+ K TR+         +NLEAG +NR  +GRKT + Y+AIA+PWPK SG AKV+L 
Sbjct: 469 LNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLF 528

Query: 484 TGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAE 543
           TG+V+KF+YG+ +YGGEP ++P + N    +++ED GYI+ FV DEK  +SE+ +V A  
Sbjct: 529 TGEVEKFIYGDNKYGGEPLFLPRDPN----SKEEDDGYILAFVHDEKEWKSELQIVNAMS 588

Query: 544 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           ++  A V+LP RVPYGFHGTF++  +L  QA
Sbjct: 589 LKLEATVKLPSRVPYGFHGTFINANDLANQA 605

BLAST of CsaV3_2G012080 vs. ExPASy TrEMBL
Match: A0A0A0LIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1)

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA
Sbjct: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of CsaV3_2G012080 vs. ExPASy TrEMBL
Match: A0A5D3CZW1 (9-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001030 PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 543/568 (95.60%), Postives = 552/568 (97.18%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQISL SP PPLT CRSSSTIETPPIRKLKVITPP PPLSLPKKVSPP R+LNPLQ FAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+YLRNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQMNR  VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of CsaV3_2G012080 vs. ExPASy TrEMBL
Match: A0A1S3CD31 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499487 PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 543/568 (95.60%), Postives = 552/568 (97.18%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
           MQISL SP PPLT CRSSSTIETPPIRKLKVITPP PPLSLPKKVSPP R+LNPLQ FAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
           SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+YLRNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQMNR  VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 569
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of CsaV3_2G012080 vs. ExPASy TrEMBL
Match: A0A6J1H4F0 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460311 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.4e-290
Identity = 494/571 (86.51%), Postives = 523/571 (91.59%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVIT--PPRPPLSLPKKV-SPPQRRLNPLQK 60
           MQISLQSP P   LCRSSST+ETPPIRKLK++T  PP PP   PK V SP  RRLNP Q 
Sbjct: 1   MQISLQSPLP---LCRSSSTVETPPIRKLKLLTLPPPSPPPPPPKVVLSPLPRRLNPFQN 60

Query: 61  FAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLR 120
            AASLLDAVESSL VDKLPKT+DPAVQISGNFSPVPEC VRHDLEIIGNLPACLRG+YLR
Sbjct: 61  LAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLR 120

Query: 121 NGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPI 180
           NGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+QE ALGR VFPKPI
Sbjct: 121 NGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPKPI 180

Query: 181 GELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKD 240
           GELHGHQGLARLAIFLARAGIGLID SKGTGVANAGLVYFNGRLLALSEDDLPYHV I+D
Sbjct: 181 GELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVLIRD 240

Query: 241 DGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRD 300
            GDLETIGRF+FN QI+CPMIAHPKVDP+SGDLHGLSYNMIKKPYLKYLRFDR G+K RD
Sbjct: 241 GGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRFDRLGRKVRD 300

Query: 301 VDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGV 360
           VDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKSGV
Sbjct: 301 VDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSGV 360

Query: 361 DEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTE 420
           DE GIVWI+VP+CFCFHLWNAWEETG N EK++V VGSCMNPPDSIFNDRDQ L IELTE
Sbjct: 361 DETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRDQALTIELTE 420

Query: 421 IRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETG 480
           IRMD+++RK TRRVFGSGMNLEAGQ+NR LVGR+TRFVYMAIA+PWPKCSGIAKVDLET 
Sbjct: 421 IRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLETR 480

Query: 481 KVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEME 540
           KVKKFLYG+GRYGGEPFYV ENGN  G++E EDGGY+VGFVRDEKRERSE+VVVKAAEM 
Sbjct: 481 KVKKFLYGDGRYGGEPFYVSENGN-SGEDENEDGGYVVGFVRDEKRERSELVVVKAAEMM 540

Query: 541 EVAAVRLPVRVPYGFHGTFVSEEELNGQARN 569
           EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 EVAAVRLPARVPYGFHGTFVSEEELKGQAPN 567

BLAST of CsaV3_2G012080 vs. ExPASy TrEMBL
Match: A0A6J1L4D2 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499033 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.8e-288
Identity = 491/571 (85.99%), Postives = 522/571 (91.42%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVIT--PPRPPLSLPKKVSPP-QRRLNPLQK 60
           MQISLQSP P   LCRSSST+ETPPIRKLK++T  PP P  S PK VS P   R+NP Q 
Sbjct: 1   MQISLQSPLP---LCRSSSTVETPPIRKLKLLTLPPPSPSPSPPKVVSTPLPHRINPFQN 60

Query: 61  FAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLR 120
            AASLLDAVESSL VDKLPKT+DPAVQISGNFSPVPEC VRHDLEIIGNLPACLRG+YLR
Sbjct: 61  LAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLR 120

Query: 121 NGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPI 180
           NGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL QE ALGR VFPKPI
Sbjct: 121 NGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLVQEVALGRPVFPKPI 180

Query: 181 GELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKD 240
           GELHGHQGLARLAIFLARAGIGLID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I+D
Sbjct: 181 GELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIRD 240

Query: 241 DGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRD 300
            GDLETIGRF+FN QI+CPMIAHPKVDPI GDLHGLSYNMIKKPYLKYLRFDR GKK RD
Sbjct: 241 GGDLETIGRFSFNSQISCPMIAHPKVDPILGDLHGLSYNMIKKPYLKYLRFDRLGKKVRD 300

Query: 301 VDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGV 360
           VDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFD  KTSRFGILPKSGV
Sbjct: 301 VDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDSNKTSRFGILPKSGV 360

Query: 361 DEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTE 420
           DE GIVWI+VP+CFCFHLWNAWEETG N EK++V VGSCMNPPDSIFNDRDQ L IELTE
Sbjct: 361 DETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRDQALTIELTE 420

Query: 421 IRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETG 480
           IRMD+++RK TRRVFGSGMNLEAGQ+NR LVGR+TRFVYMAIA+PWPKCSGIAKVDLETG
Sbjct: 421 IRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLETG 480

Query: 481 KVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEME 540
           KVKKFLYG+GR+GGEPFYV ENGN  G++E EDGGY+VGFVRDEKR+RSE+VVVKAAEM+
Sbjct: 481 KVKKFLYGDGRFGGEPFYVSENGN-SGEDENEDGGYVVGFVRDEKRQRSELVVVKAAEMK 540

Query: 541 EVAAVRLPVRVPYGFHGTFVSEEELNGQARN 569
           EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 EVAAVRLPARVPYGFHGTFVSEEELKGQAPN 567

BLAST of CsaV3_2G012080 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 708.0 bits (1826), Expect = 6.2e-204
Identity = 346/581 (59.55%), Postives = 440/581 (75.73%), Query Frame = 0

Query: 1   MQISLQSPPPPLTLCRSSSTIETPP---------IRKLKVITPP--RPPLSLPKKVSPPQ 60
           MQ SL+S   P      S  +  P          I   K+ T P    P+  P K+ P  
Sbjct: 1   MQHSLRSDLLPTKTSPRSHLLPQPKNANISRRILINPFKIPTLPDLTSPVPSPVKLKPTY 60

Query: 61  RRLNPLQKFAASLLDAVESSLFVD-----KLPKTIDPAVQISGNFSPVPECSVRHDLEII 120
             LN LQK AA++LD +ESS+ +       LPK  DPAVQ+SGNF+PV EC V++ LE++
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 121 GNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQ 180
           G +P+CL+G+Y+RNGANPM  P  GHHLFDGDGMIHAV+    N+ SYSCR+T+TNRL Q
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQ 180

Query: 181 EAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLAL 240
           E ALGR VFPKPIGELHGH GLARLA+F ARAGIGL+DG++G GVANAG+V+FNGRLLA+
Sbjct: 181 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAM 240

Query: 241 SEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLK 300
           SEDDLPY V+I   GDLETIGRF F+ QI+  +IAHPKVD  +GDLH LSYN++KKP+L+
Sbjct: 241 SEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLR 300

Query: 301 YLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPK 360
           YL+F+  GKK+RDV+ITL EPTMIHDFAITEN VVIPD Q+VFKL EM+RGGSPV++  +
Sbjct: 301 YLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKE 360

Query: 361 KTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIF 420
           K +RFG+L K  +    I W++VP+CFCFHLWNAWEE     +  IVV+GSCM+PPD+IF
Sbjct: 361 KMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIF 420

Query: 421 NDRDQPLRIELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWP 480
           ++  +P R+EL+EIR+++++++  R+V  +G+NLEAG +NR  VGRK++FVY+AIADPWP
Sbjct: 421 SESGEPTRVELSEIRLNMRTKESNRKVIVTGVNLEAGHINRSYVGRKSQFVYIAIADPWP 480

Query: 481 KCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRE 540
           KCSGIAKVD++ G V +F YG  R+GGEP +VPE     G+ E ED GY++GFVRDE+++
Sbjct: 481 KCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-----GEGE-EDKGYVMGFVRDEEKD 540

Query: 541 RSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 566
            SE VVV A +M++VAAVRLP RVPYGFHGTFVSE +L  Q
Sbjct: 541 ESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

BLAST of CsaV3_2G012080 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 648.7 bits (1672), Expect = 4.5e-186
Identity = 316/555 (56.94%), Postives = 410/555 (73.87%), Query Frame = 0

Query: 18  SSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASLLDAVESSLFVDKL-- 77
           SS++++P  +            +L  K +    +LNP+Q+ AA +LDAVE+++   +   
Sbjct: 111 SSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRR 170

Query: 78  ---PKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHL 137
              PKT DPAVQI+GNF PVPE  V H+L + G +P C++G+Y+RNGANP+H P  GHHL
Sbjct: 171 HPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHL 230

Query: 138 FDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIF 197
           FDGDGM+HAV F +G+  SY+CRFT TNRL QE   GR VFPK IGELHGH G+A+L +F
Sbjct: 231 FDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLF 290

Query: 198 LARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQ 257
             R   GL+D + G GVANAGLVYFNG LLA+SEDDLPYHV++   GDLET GR++F+GQ
Sbjct: 291 NTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQ 350

Query: 258 INCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFA 317
           +   MIAHPK+DP + +L  LSY+++ KPYLKY RF   G+KS DV+I L +PTMIHDFA
Sbjct: 351 LKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFA 410

Query: 318 ITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFC 377
           ITEN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+  D   I WIEVP+CFC
Sbjct: 411 ITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFC 470

Query: 378 FHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR-V 437
           FHLWN+WEE    +   +VV+GSCM PPDSIFN+ D+ L+  L+EIR+++K+ + TRR V
Sbjct: 471 FHLWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPV 530

Query: 438 FGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGG 497
               +NLEAG +NR L+GRKTR+ Y+A+ +PWPK SG AKVDL TG+++K++YGEG+YGG
Sbjct: 531 ISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGG 590

Query: 498 EPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYG 557
           EP ++P      GD E EDGGYI+ FV DE++ +SE+ ++ A  M+  A V LP RVPYG
Sbjct: 591 EPLFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYG 650

Query: 558 FHGTFVSEEELNGQA 567
           FHGTF+S+E+L+ QA
Sbjct: 651 FHGTFISKEDLSKQA 655

BLAST of CsaV3_2G012080 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 644.8 bits (1662), Expect = 6.4e-185
Identity = 318/557 (57.09%), Postives = 403/557 (72.35%), Query Frame = 0

Query: 18  SSTIETPPIRKLKVITPPRPPLSL-PKKVSPPQRRLNPLQKFAASLLDAVESSLF----V 77
           SS + TPP       +   P + + PK      +++N  Q+ AA+ LDA E  L     +
Sbjct: 51  SSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKL 110

Query: 78  DKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHL 137
             LPKT DP+VQI+GNF+PV E  VR +L ++G LP  ++G+Y+RNGANP+H P  GHH 
Sbjct: 111 HPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHF 170

Query: 138 FDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIF 197
           FDGDGM+HAV F  G+ ASY+CRFT+TNR  QE  LGR VFPK IGELHGH G+ARL +F
Sbjct: 171 FDGDGMVHAVKFEHGS-ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 230

Query: 198 LARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQ 257
            ARA  G++D + GTGVANAGLVYFNGRLLA+SEDDLPY VQI  +GDL+T+GRF+F+GQ
Sbjct: 231 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 290

Query: 258 INCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFA 317
           +   MIAHPKVDP SG+L  LSY+++ KPYLKY RF   G KS DV+I L +PTM+HDFA
Sbjct: 291 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 350

Query: 318 ITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFC 377
           ITEN VV+PD QVVFKL EM+RGGSPVV+D  K +RFGIL K   D   I WI+ P+CFC
Sbjct: 351 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 410

Query: 378 FHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVF 437
           FHLWNAWEE    +   +VV+GSCM PPDSIFN+ D+ L+  L+EIR+++K+ + TRR  
Sbjct: 411 FHLWNAWEEP---ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPI 470

Query: 438 GSG----MNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGR 497
            S     +NLEAG +NR ++GRKT+F Y+A+A+PWPK SG AKVDL TG+VKK LYG+ R
Sbjct: 471 ISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNR 530

Query: 498 YGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRV 557
           YGGEP ++P      G+  +ED GYI+ FV DEK  +SE+ +V A  +E  A V+LP RV
Sbjct: 531 YGGEPLFLP------GEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRV 590

Query: 558 PYGFHGTFVSEEELNGQ 566
           PYGFHGTF+  ++L  Q
Sbjct: 591 PYGFHGTFIGADDLAKQ 597

BLAST of CsaV3_2G012080 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 629.0 bits (1621), Expect = 3.7e-180
Identity = 307/529 (58.03%), Postives = 386/529 (72.97%), Query Frame = 0

Query: 46  SPPQR---RLNPLQKFAASLLDAVESSLFVDK----LPKTIDPAVQISGNFSPVPECSVR 105
           +PPQ    RLN  QK AA  +DA E +L   +    LPKT DP VQI+GN+SPVPE SVR
Sbjct: 60  TPPQHNPLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVR 119

Query: 106 HDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTR 165
            +L + G +P C+ G+Y+RNGANPM  PT GHHLFDGDGM+HAV   +G+ ASY+CRFT+
Sbjct: 120 RNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGS-ASYACRFTK 179

Query: 166 TNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFN 225
           T RL QE  LGR VFPK IGELHGH G+ARL +F AR   GLI+   G GVANAGLVYFN
Sbjct: 180 TERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFN 239

Query: 226 GRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMI 285
            RLLA+SEDDLPY ++I   GDL+T+GR++F+GQ+   MIAHPK+DP++ +LH LSY+++
Sbjct: 240 NRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVV 299

Query: 286 KKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSP 345
           KKPYLKY RF   G KS +++I L  PTMIHDFAITEN VVIPD QVVFKL EM+ G SP
Sbjct: 300 KKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSP 359

Query: 346 VVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMN 405
           VVFD +K SR GI+PK   +   I+W+  P  FCFHLWNAWE     + + IVV+GSCM+
Sbjct: 360 VVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESP---ETEEIVVIGSCMS 419

Query: 406 PPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR--VFGSGMNLEAGQMNRGLVGRKTRFVY 465
           P DSIFN+RD+ LR  L+EIR+++++RK TRR  +    +NLE G +NR  +GRKTRF +
Sbjct: 420 PADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLVNEDVNLEIGMVNRNRLGRKTRFAF 479

Query: 466 MAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVG 525
           +AIA PWPK SG AKVDL TG++KK++YG  +YGGEPF++P  GN G   E ED GYI  
Sbjct: 480 LAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLP--GNSGNGEENEDDGYIFC 539

Query: 526 FVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 566
            V DE+ + SE+ ++ A  ++  A ++LP RVPYGFHGTFV   EL  Q
Sbjct: 540 HVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of CsaV3_2G012080 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 616.3 bits (1588), Expect = 2.5e-176
Identity = 312/571 (54.64%), Postives = 400/571 (70.05%), Query Frame = 0

Query: 3   ISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAASL 62
           +S  +  P      ++S  +TP +  L     P P   +P+K      R NPLQ+ A++ 
Sbjct: 32  VSFTNTKPRRRKLSANSVSDTPNL--LNFPNYPSPNPIIPEK---DTSRWNPLQRAASAA 91

Query: 63  LDAVESSLF----VDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRN 122
           LD  E++L        LPKT+DP  QISGN++PVPE SV+  L + G +P C+ G+YLRN
Sbjct: 92  LDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRN 151

Query: 123 GANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIG 182
           GANP+  P  GHHLFDGDGM+HAV   +G+ ASYSCRFT T RL QE  LG  +FPK IG
Sbjct: 152 GANPLFEPVSGHHLFDGDGMVHAVKITNGD-ASYSCRFTETERLVQEKQLGSPIFPKAIG 211

Query: 183 ELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDD 242
           ELHGH G+ARL +F AR   GL++   GTGVANAGLVYF+ RLLA+SEDDLPY V++ D+
Sbjct: 212 ELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDN 271

Query: 243 GDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDV 302
           GDLETIGRF+F+GQ++  MIAHPK+DP++ +L  LSY+++KKPYLKY +F   G+KS DV
Sbjct: 272 GDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDV 331

Query: 303 DITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVD 362
           +I L  PTM+HDFAITEN VVIPD QVVFKL +M  G SPV +D +K SRFGILP++  D
Sbjct: 332 EIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKD 391

Query: 363 EKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEI 422
              +VW+E P  FCFHLWNAWE     +   +VV+GSCM P DSIFN+ D+ L   L+EI
Sbjct: 392 ASEMVWVESPETFCFHLWNAWESP---ETDEVVVIGSCMTPADSIFNECDEQLNSVLSEI 451

Query: 423 RMDVKSRKVTRRVFGSG---MNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLE 482
           R+++K+ K TRR    G   MNLEAG +NR L+GRKTR+ Y+AIA+PWPK SG AKVDL 
Sbjct: 452 RLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLS 511

Query: 483 TGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAE 542
           TG+VK   YG  +YGGEPF++P     G +++ ED GYI+ FV DE+   SE+ +V A  
Sbjct: 512 TGEVKNHFYGGKKYGGEPFFLPR----GLESDGEDDGYIMSFVHDEESWESELHIVNAVT 571

Query: 543 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           +E  A V+LP RVPYGFHGTFV+  ++  QA
Sbjct: 572 LELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004150345.10.0e+00100.009-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis sativus] >KGN615... [more]
XP_008460725.10.0e+0095.60PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis melo]... [more]
XP_038875554.10.0e+0091.909-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida][more]
KAG6574873.12.2e-29187.029-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyr... [more]
XP_023548938.16.3e-29186.569-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
Q9LRM78.7e-20359.559-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana O... [more]
Q5MBR56.3e-18559.519-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q9M9F56.3e-18556.949-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O... [more]
O245928.2e-18557.359-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 P... [more]
O240231.1e-18457.099-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Match NameE-valueIdentityDescription
A0A0A0LIE20.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1[more]
A0A5D3CZW10.0e+0095.609-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CD310.0e+0095.609-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A6J1H4F03.4e-29086.519-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1L4D21.8e-28885.999-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT3G24220.16.2e-20459.55nine-cis-epoxycarotenoid dioxygenase 6 [more]
AT1G78390.14.5e-18656.94nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G14440.16.4e-18557.09nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT4G18350.13.7e-18058.03nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G30100.12.5e-17654.64nine-cis-epoxycarotenoid dioxygenase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 94..558
e-value: 3.9E-115
score: 385.7
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 46..565
NoneNo IPR availablePANTHERPTHR10543:SF1019-CIS-EPOXYCAROTENOID DIOXYGENASE NCED6, CHLOROPLASTICcoord: 46..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G012080.1CsaV3_2G012080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen