CsaV3_1G037530 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037530 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 535/535 (100.00%), Postives = 535/535 (100.00%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120
           RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240
           HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG
Sbjct: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK
Sbjct: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS
Sbjct: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 535

BLAST of CsaV3_1G037530 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1020.4 bits (2637), Expect = 5.7e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK FPLNPL   SFT+VSL STQWSTASSYKNKDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CsaV3_1G037530 vs. NCBI nr
Match: XP_038895165.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 942.2 bits (2434), Expect = 2.0e-270
Identity = 457/536 (85.26%), Postives = 495/536 (92.35%), Query Frame = 0

Query: 1   MKFFPLNPLF-LISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSI 60
           MK FPLNPL    +F IVS F  QW+ ASSYK++DFLQCLSI+S  IPIYTPINSSYSS+
Sbjct: 1   MKVFPLNPLLTFTNFIIVSSFLVQWTAASSYKHEDFLQCLSIYSRDIPIYTPINSSYSSL 60

Query: 61  LRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYI 120
           L SYAQN+RFSTQ+KTLKPLVIIKPSH+SH+Q+ VICSKSH LQIRIRSGGHDTEGLSYI
Sbjct: 61  LTSYAQNTRFSTQHKTLKPLVIIKPSHISHIQAIVICSKSHGLQIRIRSGGHDTEGLSYI 120

Query: 121 SDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 180
           SD+PF+V+DLINLKSIEVDT NNTAWVQSGATIGELYY+IAEKSRTLAFPAGVCPTVGIG
Sbjct: 121 SDVPFIVIDLINLKSIEVDTKNNTAWVQSGATIGELYYKIAEKSRTLAFPAGVCPTVGIG 180

Query: 181 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVV 240
           GHFSGGGYGWLMRKYGLAADNV+DAYLVD +G VFDRE MGEDLFWAIRGGGGGSFGIVV
Sbjct: 181 GHFSGGGYGWLMRKYGLAADNVMDAYLVDDDGRVFDRETMGEDLFWAIRGGGGGSFGIVV 240

Query: 241 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVK 300
           GWKVKLVRVPPIVTTCRL+KSLDKNTKKIVYQWQYVAN+ME+ LLIGINLTGGNPT+GVK
Sbjct: 241 GWKVKLVRVPPIVTTCRLEKSLDKNTKKIVYQWQYVANKMEDTLLIGINLTGGNPTQGVK 300

Query: 301 RNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEIL 360
           RNPTASFFSL+LGKT+KL+TIMNTT P LGLTK+NC+ETSWIQSTLI+AGFTNG+ LE+L
Sbjct: 301 RNPTASFFSLFLGKTNKLITIMNTTFPELGLTKSNCEETSWIQSTLISAGFTNGEALEVL 360

Query: 361 LSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSS 420
           L+KP +   ISYKIKSDYVKQPISQ A KGIW+RLKSQ  ETSQLFLFPYGGKMS ISSS
Sbjct: 361 LAKPPI-KTISYKIKSDYVKQPISQQALKGIWERLKSQVTETSQLFLFPYGGKMSKISSS 420

Query: 421 KTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDL 480
           K PFSHRAEFLYKISYTVGW EQ   ANERHLNWIRE Y +MTPFVSNSPRAAY+NYRDL
Sbjct: 421 KIPFSHRAEFLYKISYTVGWTEQSIVANERHLNWIRELYDYMTPFVSNSPRAAYINYRDL 480

Query: 481 DIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           DIGTNNKYG TSY++ASIWGLKYFGNNFK+LVQVKT VDPSNFFRHEQSIPPL+LS
Sbjct: 481 DIGTNNKYGNTSYKQASIWGLKYFGNNFKRLVQVKTKVDPSNFFRHEQSIPPLSLS 535

BLAST of CsaV3_1G037530 vs. NCBI nr
Match: XP_023520034.1 (tetrahydrocannabinolic acid synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.6 bits (2030), Expect = 1.4e-223
Identity = 386/533 (72.42%), Postives = 443/533 (83.11%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S FS  W  A ++K++ FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFSVLWVVALAHKHEHFLQCLSIHSRDISKVIYTPINSSYSSLLSSY 65

Query: 66  AQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS-DL 125
           A N R ST   T  P VIIKPSH+SH+Q+ V+CSKSH  +IR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSTP-ATPNPRVIIKPSHLSHIQAAVVCSKSHGFRIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF+VVDL NL SI VD  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIVVDLKNLNSIAVDIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTEGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG + +L+  MNTT P LGLT  +C++TSWIQSTL+AAGFTN  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHQLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTNRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSQIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   ANER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSMEANERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHEQS+PPLTLS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLTLS 531

BLAST of CsaV3_1G037530 vs. NCBI nr
Match: XP_022927076.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 776.5 bits (2004), Expect = 1.4e-220
Identity = 380/533 (71.29%), Postives = 441/533 (82.74%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S F+  W+ A ++K++ FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSY 65

Query: 66  AQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS-DL 125
           A N R S    T  P VIIKPSH+SH+Q+ V+CSKSH  QIR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+AAGFT+  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of CsaV3_1G037530 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.4e-141
Identity = 262/529 (49.53%), Postives = 348/529 (65.78%), Query Frame = 0

Query: 20  FSTQWSTASSYKNKDFLQCLSIHSTHIP--------IYTPINSSYSSILRSYAQNSRFST 79
           F  Q S A+  +N  FL+C    S HIP        +YT  +  Y SIL S  QN RF +
Sbjct: 21  FHIQISIANPREN--FLKCF---SKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFIS 80

Query: 80  QYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYISDLPFVVVDLIN 139
              T KPLVI+ PS+ SH+Q+T++CSK   LQIR RSGGHD EG+SYIS +PFVVVDL N
Sbjct: 81  D-TTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRN 140

Query: 140 LKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLM 199
           + SI++D  + TAWV++GAT+GE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG LM
Sbjct: 141 MHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALM 200

Query: 200 RKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVRVPPI 259
           R YGLAADN+IDA+LV+ +G V DR++MGEDLFWAIRGGGG +FGI+  WK+KLV VP  
Sbjct: 201 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSK 260

Query: 260 VTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFF 319
            T   + K+++     K+  +WQ +A + ++ L++  +    N T    +N T     F 
Sbjct: 261 STIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFS 320

Query: 320 SLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKP 379
           S++ G  D LV +MN + P LG+ K +CKE SWI +T+  +G  N        EILL + 
Sbjct: 321 SIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR- 380

Query: 380 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPF 439
           +     ++ IK DYVK+PI + A   I ++L  ++V      L+PYGG M  IS S  PF
Sbjct: 381 SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPF 440

Query: 440 SHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGT 499
            HRA  +Y++ YT  W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G 
Sbjct: 441 PHRAGIMYELWYTASWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 500

Query: 500 NNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPL 533
            N     +Y +A IWG KYFG NF +LV+VKT VDP+NFFR+EQSIPPL
Sbjct: 501 TNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CsaV3_1G037530 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 6.8e-141
Identity = 261/530 (49.25%), Postives = 352/530 (66.42%), Query Frame = 0

Query: 16  IVSLFSTQWSTASSYKNKDFLQCLSIH----STHIP-IYTPINSSYSSILRSYAQNSRFS 75
           I   FS    T+ +   ++FL+C S +    +T++  +YT  N  Y S+L S   N RF 
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRF- 74

Query: 76  TQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYISDLPFVVVDLI 135
           T   T KPLVI+ PSHVSH+Q T++CSK   LQIR RSGGHD+EG+SYIS +PFV+VDL 
Sbjct: 75  TSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLR 134

Query: 136 NLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWL 195
           N++SI++D  + TAWV++GAT+GE+YY + EK+  L+  AG CPTV  GGHF GGGYG L
Sbjct: 135 NMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPL 194

Query: 196 MRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVRVPP 255
           MR YGLAADN+IDA+LV+ +G V DR++MGEDLFWA+RGGG  SFGI+V WK++LV VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK 254

Query: 256 IVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTA---SF 315
             T   + K ++     K+V +WQ +A + ++ LL+  +    N T    +N TA    F
Sbjct: 255 -STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYF 314

Query: 316 FSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQP----LEILLSK 375
            S++LG  D LV +MN + P LG+ K +C++ SWI + +  +G  N        EILL +
Sbjct: 315 SSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDR 374

Query: 376 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTP 435
            +   N ++KIK DYVK+PI +  F  I ++L  +++      L+PYGG M  IS S  P
Sbjct: 375 -SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 434

Query: 436 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIG 495
           F HRA  LY++ Y   W +Q    NE+HLNWIR  Y+FMTP+VS +PR AY+NYRDLDIG
Sbjct: 435 FPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 494

Query: 496 TNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPL 533
            N+     +Y +A IWG KYFG NF +LV+VKT VDP+NFFR+EQSIPPL
Sbjct: 495 INDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CsaV3_1G037530 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.0e-140
Identity = 261/528 (49.43%), Postives = 348/528 (65.91%), Query Frame = 0

Query: 20  FSTQWSTASSYKNKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNSRFSTQYK 79
           F+ Q S A+  +N  FL+C S +  T++     +YT  +  Y SIL S  QN RF T   
Sbjct: 21  FNIQISIANPQEN--FLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVII P +VSH+Q T++CSK   LQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV++GAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGEDLFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNK 499
           A  +Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF +LV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CsaV3_1G037530 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.0e-140
Identity = 262/528 (49.62%), Postives = 347/528 (65.72%), Query Frame = 0

Query: 20  FSTQWSTASSYKNKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNSRFSTQYK 79
           F+ Q S A+  +N  FL+CLS +  T++     +YT  +  Y SIL S  QN RF T   
Sbjct: 21  FNIQISIANPQEN--FLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI  P +VSH+Q T++CSK   LQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV+SGAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGEDLFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNK 499
           A   Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF +LV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CsaV3_1G037530 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.4e-140
Identity = 257/526 (48.86%), Postives = 346/526 (65.78%), Query Frame = 0

Query: 20  FSTQWSTASSYKNKDFLQCLSIHSTHIP-----IYTPINSSYSSILRSYAQNSRFSTQYK 79
           F+ Q S A+  +N  FL+C S +  + P     IYT  +  Y S+L S  QN RF T   
Sbjct: 21  FNIQISIANPQEN--FLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI+ PS+VSH+Q++++CSK   LQIR RSGGHD EGLSYIS +PF +VDL N+ +
Sbjct: 81  TPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHT 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           ++VD  + TAWV++GAT+GE+YY I E +   +FP G CPTVG+GGHFSGGGYG LMR Y
Sbjct: 141 VKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGEDLFWAIRGGGG +FGI+  WK+KLV VP   T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+  +WQ +A + ++ L++  +    N T    +N T     F S++
Sbjct: 261 FSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
           LG  D LV +MN + P LG+ K +CKE SWI +T+  +G  N        EILL + +  
Sbjct: 321 LGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDR-SAG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPFSHR 439
              ++ IK DYVK+ I + A   I ++L  +EV      L+PYGG M  IS S  PF HR
Sbjct: 381 KKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIGTNNK 499
           A  +Y++ YT  W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNP 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPL 533
               +Y +A IWG KYFG NF +LV+VKT  DP+NFFR+EQSIPPL
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CsaV3_1G037530 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 2.8e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK FPLNPL   SFT+VSL STQWSTASSYKNKDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CsaV3_1G037530 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 2.8e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK FPLNPL   SFT+VSL STQWSTASSYKNKDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM+NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAYVNYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CsaV3_1G037530 vs. ExPASy TrEMBL
Match: A0A6J1EGP1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111434010 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.9e-221
Identity = 380/533 (71.29%), Postives = 441/533 (82.74%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S F+  W+ A ++K++ FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSY 65

Query: 66  AQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS-DL 125
           A N R S    T  P VIIKPSH+SH+Q+ V+CSKSH  QIR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+AAGFT+  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+MS I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVSNSPRAAYVNYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of CsaV3_1G037530 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 3.1e-197
Identity = 348/541 (64.33%), Postives = 429/541 (79.30%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIP-----IYTPINSS 60
           MK+ PL  L L +F ++S  S+ W+ AS+ K++ FLQCLS HS         IYTPIN S
Sbjct: 1   MKYSPLTILSL-AFILLS-SSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPS 60

Query: 61  YSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEG 120
           YSS+L    +N RFS+  +T KPL+II PS+VSH+Q+ VICSK+H LQIRIRSGGHD EG
Sbjct: 61  YSSVLNFSVRNLRFSSA-ETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 180
           LSY++ LPF+++DLINLKSI V+  N+TAW+QSGAT+GELYY IA+KSRTLAFPAGVCPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSF 240
           VG+GGHFSGGGYG ++RKYGLAADNVIDAYL+DANG  +DRE+MGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPT 300
           GIVV WKVKLV VP IVTTC  D++LD++T  ++++WQ VA +++E L  GI L GGN +
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNAS 300

Query: 301 K--GVKR-NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTN 360
              GVK+ NPTA+FFSL+LGK+D+L+ I+NT  P L L K +C ETSWI+STL+AA    
Sbjct: 301 TEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQ 360

Query: 361 G-QPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGG 420
             + LE LL++  L+   + K+KSDYVK+PI +   +GIW+RLK+Q++E  QL   PYGG
Sbjct: 361 TIESLESLLNRTPLTIE-NTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGG 420

Query: 421 KMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRA 480
           +MS IS S+TPFSHRA  LYKI Y VGW EQ   A ERHL+WIRE Y +MTPFVS SPRA
Sbjct: 421 RMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPP 533
            YVNYRDLDIGTNNKYGKTSY++AS+WG KYFGNNF +LV +K+ VDPSNFF++EQSIPP
Sbjct: 481 TYVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPP 537

BLAST of CsaV3_1G037530 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 7.7e-196
Identity = 340/538 (63.20%), Postives = 428/538 (79.55%), Query Frame = 0

Query: 1   MKFFPLNPL-FLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIP-----IYTPINS 60
           MK FPLNPL F  + +IV   S  W+ AS++K++ FLQCL+ HS         IYTP N 
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWA-ASAHKHEGFLQCLTRHSPSNNSISKFIYTPANF 60

Query: 61  SYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTE 120
           SYSSIL    QN RFST  +T KPL++I PSH+SH+Q+ VICSK+H LQIR RSGGHD E
Sbjct: 61  SYSSILNFSIQNLRFSTA-ETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFE 120

Query: 121 GLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCP 180
           GLSY+S LPF+V+DLI+L+SI +D  N+TAWVQSGAT+GELYYRIA+KS+TLAFPAG CP
Sbjct: 121 GLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCP 180

Query: 181 TVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGS 240
           TVG+GGH SGGGYG+L+RKYGLAAD+VIDA+LVDANG + +RE+M +DLFW IRGGGGGS
Sbjct: 181 TVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGS 240

Query: 241 FGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGN- 300
           FGIVV WK++LV VP  +TTC ++++LD++  K++++WQYVA+++E+ LL+GI+LTGG+ 
Sbjct: 241 FGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHI 300

Query: 301 -PTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTN 360
              +G K NP ASF + +LGK D+LV I+NTT P LGLTK +C ETSWI+STLI AGF+ 
Sbjct: 301 LTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360

Query: 361 GQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGK 420
           G+ LE LL +  L  NIS KIKSDYVK+PIS+  FKGIW+RL +Q++E +Q+   PYGG+
Sbjct: 361 GESLETLLYRRPLV-NISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGR 420

Query: 421 MSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAA 480
           MS  S S+TP+ HRAE +YK+ Y V W EQ + A +RHLNW+RE Y +M P+V+ SPRAA
Sbjct: 421 MSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAA 480

Query: 481 YVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIP 531
           YVNYRDLDIG NN+Y KT+YE A +WGLKYFG NF +LVQVKT VDPS+FFRHEQSIP
Sbjct: 481 YVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535

BLAST of CsaV3_1G037530 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.6e-137
Identity = 249/542 (45.94%), Postives = 348/542 (64.21%), Query Frame = 0

Query: 8   PLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPIN----------SSYS 67
           PLF I     SL++   +++ +     F+ C+   +TH  +Y P+           S +S
Sbjct: 8   PLFSILVLYFSLYTITPTSSLASLQDQFINCVQ-RNTH--VYFPLEKTFFAPTKNVSMFS 67

Query: 68  SILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLS 127
            +L S AQN RF  +    KP  I  P H SH+Q+++ICSK   + +R+RSGGHD EGLS
Sbjct: 68  QVLESTAQNLRF-LKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 127

Query: 128 YIS--DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 187
           Y+S  D PF+++DL  ++ + ++  +N+AWVQSGAT+GELYYRIAEKS+   FPAG+C +
Sbjct: 128 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 187

Query: 188 VGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSF 247
           +GIGGH +GG YG +MRKYGL ADNV+DA +VDANG + DR AMGED FWAIRGG GGSF
Sbjct: 188 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 247

Query: 248 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNT-KKIVYQWQYVANRMEEKLLIGINL----T 307
           GI++ WK+KLV VP  VT   + K+L ++   KI+ +WQ VA+++ E+L I +      T
Sbjct: 248 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 307

Query: 308 GGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGF 367
           GGN T       T S+ +L+LG    L+ +M  + P LGLT  +C E SW++S    +GF
Sbjct: 308 GGNKT------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 367

Query: 368 TNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYG 427
               P  +LL   +    +S+K KSD+VK PI +   +GI+ +L  +++    +   PYG
Sbjct: 368 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 427

Query: 428 GKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPR 487
           G M+ I  S+ PF HR   L+K+ Y   W +     + RH+NWIR+ YS+MTP+VS++PR
Sbjct: 428 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPS-RHINWIRDLYSYMTPYVSSNPR 487

Query: 488 AAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIP 533
            AYVNYRDLD+G N K  KT  ++A +WG  YF NNF +L+ +K  VDP NFFRHEQSIP
Sbjct: 488 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of CsaV3_1G037530 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 3.1e-136
Identity = 250/530 (47.17%), Postives = 344/530 (64.91%), Query Frame = 0

Query: 10  FLISFTIVSLFSTQWSTASSYKNKDFLQCLSIH---STHIP--IYTPINSSYSSILRSYA 69
           FL +  + SL  +   +A    ++DFL+CLS     +T  P  I+T  +SS+ SIL S  
Sbjct: 6   FLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 65

Query: 70  QNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS-DLP 129
           QN RFS   +T KP+ II P   S +Q+ + C++ H + +R RS GH  EGLSYI+ + P
Sbjct: 66  QNPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 125

Query: 130 FVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFS 189
           F V+DL NL+SI +D  N T WVQ+GAT GELYY I + +++LAFPAG+ PTVG+GG FS
Sbjct: 126 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 185

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG L+RKYGLAADN+IDA +VDA+G + DR+AMGED FWAIRGGGG SFG+++ WKV
Sbjct: 186 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 245

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT 309
           KLV VP  +T  ++ K+  K   +I+ +WQY A+++ + L I   L   N     K    
Sbjct: 246 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-----KNAVH 305

Query: 310 ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKP 369
           A F  LY+G  + L+ +M    P LGL K  C+E SWI+S L  A F  G+ L +L ++ 
Sbjct: 306 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE 365

Query: 370 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKTPF 429
             S  +S+K K D+V++PI + A + IW RL++ E    ++ L P+GGKMS ++  +TPF
Sbjct: 366 RTS--LSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 425

Query: 430 SHRAEFLYKISYTVGWAEQ---GSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 489
            HR   LY+I Y   W E+        +++L W+   Y FMTP+VS SPR AYVN++D+D
Sbjct: 426 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 485

Query: 490 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIP 531
           +G      KT YEE   WG+KYF NNF++LV+VKT VDP++FF  EQSIP
Sbjct: 486 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CsaV3_1G037530 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 482.6 bits (1241), Expect = 4.0e-136
Identity = 246/532 (46.24%), Postives = 353/532 (66.35%), Query Frame = 0

Query: 9   LFLISFTIVSLFSTQWSTASSYKNKDFLQCLSI-----HSTHIPIYTPINSSYSSILRSY 68
           L L+ F +V ++    S++SS  ++ F QCL+      H     I+   N SYSS+L++ 
Sbjct: 5   LILVLFFVVFIWQ---SSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 64

Query: 69  AQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYI--SD 128
            +N RF+T   T KP +II  +H SH+Q+ + C K H+LQ++IRSGGHD +GLSY+  S 
Sbjct: 65  IRNLRFNTT-STPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 124

Query: 129 LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGH 188
            PF V+D+ NL+S++VD  + TAWVQ+GA +GE+YY I EKS+TLA+PAG+CPTVG+GGH
Sbjct: 125 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 184

Query: 189 FSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVGW 248
            SGGGYG +MRKYGL  DN IDA +VD NG + DR+ MGEDL+WAI GGGGGS+G+V+ +
Sbjct: 185 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 244

Query: 249 KVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRN 308
           K+ LV VP  VT  R+ ++L++N   I+++WQ VA ++ ++L I   +   N T   ++ 
Sbjct: 245 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKT 304

Query: 309 PTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLS 368
              +F +++LG T  L++I+N   P LGL +++C ETSWIQS L       G    +LL 
Sbjct: 305 VRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQ 364

Query: 369 KPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSKT 428
           +    N +  K KSDYV++PIS+   + IW ++   E+ T  +   PYGG+M  ISS+ T
Sbjct: 365 RNQPVNYL--KRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRISSTVT 424

Query: 429 PFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLDI 488
           PF +RA  L+KI Y   W ++     +R++   R+ Y FMTPFVS +PR ++ NYRD+D+
Sbjct: 425 PFPYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDL 484

Query: 489 GTNNKYGK-TSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPL 533
           G N+  GK +SY E   +G KYF  NF++LV++KT VD  NFFR+EQSIP L
Sbjct: 485 GINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CsaV3_1G037530 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 479.2 bits (1232), Expect = 4.4e-135
Identity = 251/536 (46.83%), Postives = 353/536 (65.86%), Query Frame = 0

Query: 10  FLISFTIVSLFSTQWSTASSYKNKD-FLQCLSIHS------THIPIYTPINSSYSSILRS 69
           F +S  +V L     S +SS  +KD FL C+S HS          I+ P +  Y+   +S
Sbjct: 7   FALSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQS 66

Query: 70  -YAQNSRFST-QYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 129
             +QN RF T  + + KP++I+ P   + +Q +++CS+   +++R +SGGHD EGLSY+S
Sbjct: 67  LISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLS 126

Query: 130 -DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 189
              PF+++DL+N++SIE++  + TAWV +GATIGELYY+IA+ S+   FPAG CP+VG+G
Sbjct: 127 LHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVG 186

Query: 190 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFD-REAMGEDLFWAIRGGGGGSFGIV 249
           GHFSGGG+G +MRK+GLAADNV+DA  VDANG +++ R  MGEDLFWAIRGGG  SFG+V
Sbjct: 187 GHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVV 246

Query: 250 VGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG--INLTGGNPTK 309
           + WKVKLVRVP  VT  R +  L +N  KIV++WQ +A  +++ L I   ++++GG    
Sbjct: 247 LSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG---- 306

Query: 310 GVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 369
               +   +F + YLG  DKL+ +MN   P LGLT  +C E +WI S ++   +  GQPL
Sbjct: 307 ----SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDS-IMYFNWKKGQPL 366

Query: 370 EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNI 429
           E LL +    N++ +K KSD+VK PI +   +GIW R    EVE+  + + P GGKM  I
Sbjct: 367 ETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEPLGGKMYEI 426

Query: 430 SSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNY 489
             ++TPF HR   LY I Y V W  +  G  E+H+ W+R  Y +M  +VS SPR AY+NY
Sbjct: 427 GETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNY 486

Query: 490 RDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPL 533
           RDLD+G N     TS+E+A +WG +YFG+NFK+L  VK  +DP+NFFR+EQS+PPL
Sbjct: 487 RDLDLGMNRGV-NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of CsaV3_1G037530 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 476.5 bits (1225), Expect = 2.9e-134
Identity = 251/540 (46.48%), Postives = 348/540 (64.44%), Query Frame = 0

Query: 1   MKFFPLNPLFLISFTIVSLFST--QWSTASSYKNKDFLQCLS--IHSTHI---PIYTPIN 60
           MKF       LI FT  +L  +     +A+      FLQCLS  ++ ++I    I+TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  SSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDT 120
           +S+SS+L S  QN RFS      KP++I+ P   S +QS V C++   + IR RSGGHD 
Sbjct: 61  TSFSSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 120

Query: 121 EGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVC 180
           EGLSY++  PFV++DL NL+SI VD  N + WVQ+GATIGELYY I +K+RTLAFPAGVC
Sbjct: 121 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 180

Query: 181 PTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGG 240
           PTVG+GGHFSGGGYG L+RK+GLAAD+VIDA +VDA G + +R  MGED FWAIRGGGG 
Sbjct: 181 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 240

Query: 241 SFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGN 300
           SF +V+ WK+ L+ VP  VT   + K  +++  KI+++WQ+VA+++ + L I + L    
Sbjct: 241 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML---- 300

Query: 301 PTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNG 360
             +  K    ASF  LYLG    L+ ++N   P LGL + +C E SWI+S +  A     
Sbjct: 301 --QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GE 360

Query: 361 QPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM 420
           +P+ +L  +   S  +++K KSD+V++P+ + A   +W RL+  E E +QL   P+GGKM
Sbjct: 361 EPINVLTKRTRAS--LAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKM 420

Query: 421 SNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAY 480
           S I+  +TPF HR   +Y+I Y   W        E+++ W+   Y  M+ FV+ SPR AY
Sbjct: 421 SEIADYETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAY 480

Query: 481 VNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLT 534
           +N RDLD+G      ++ YEE   WG+KYF NNF++LV+VKT+VDPS+FF  EQSIPP T
Sbjct: 481 INLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527