CsGy7G020340 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G020340
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr7: 22735141 .. 22740005 (+)
RNA-Seq ExpressionCsGy7G020340
SyntenyCsGy7G020340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTATTGGGCATTTGGGTGTCTATATATTAAGCCCTTTACTCTTTTCCACCAACACTCACCATCATTTTCAAATCTCCGTCTCTTCATTCACAAAAATGAAAGAGACCGACGCCATGGAACAACCCATCGCCGCCGGCGCCGGAGTCTCAAACTCGAGCGACCTCCAAAAAACCCAAAAAAGTTCGAAGAAAAAGCTTCTAATCTTAACCCTTTGCTCCACTCTCCTCATCGTCGGTGCCGTCATTGGCATCGTTGCCGGAACAACAAAATCGTCGTCGAAATACTCCGGCGAGTCCCACGCCATTGTGAAGAGCACGTGCAGCAGCACGCTGTATCCGGACATGTGCTATTCTGCAATCTCGAGAAGTGAAGGAGCAATGTTGAAAGTGAAGAATCAAAAGGATGTGATTGAAATTTCGATTAATATTACGGTGAAAGCTGTGGAGGAAAATTACTTTAGAGTGAAGAAACTGTGTGGGTTGAAGAATATTAGTCATAGGGAGAGAATTGCTCTTCACGATTGTCTTGAGACGATTGATGAAACGCTTGACGAACTTCATAAAGCTATTGTGGATCTTAACGAATACCCAAATAAAAAGTCTCTTAACCAACATGCTGATGATCTCAAGACCTTACTTAGTTCCGCTATTACTAATCAGGTAACCCTTTCATTTTTTATCTTCTATAATCACCTTTTTTCTTTCTAACTATGTTAGTTTGTAATTTAGGATATTTTGAAAAGTAGTACAATAAACTAAAATATTTACAACATATGACAAAATTTTGAATTCTATCATTGATAGACGTATATTATTGATAGAAGTGTACGAGCGTCTACCAATATCTATTATTGATATAATTTAAAATTTTACTATATTATGTAAATATTTCGTCAAATTTGTCAAAATTGACGGTTATTCTATTTTTAAAAGTTAATCATTTTTTAAAATGAACTTTAATCTAACAAATGAGAATTATATATGAAAATTTGAATCTAATTCCTTAAACATAAGTATACATGAATTATTATTTCCCATCGTAGCAATTATTTACTTCTGTATTTGGCCAACTTTCTCATTTTAGTATTTCAATGCTTTAAAAACACCTATGTTTGTTTGGCCATATTATATGTTTCAATTTTGAGAGGATGGCATCTATTTATTAAATGAAAATACTATAGATTAAAAGATTAAATCATATATGTTTAATTGTTTCACAGTAATAACTTAACTATTATTACATGTAAATTAAAATATTTGATCCGTTTAATTTCTTCCCTAATACATTTACCATTACAAAACAACAATTAACTTTTGAAAAATTGACACGAAATGCAAAATTCGAACCATGATTAAAAGCTAAATCTAAAGTCATAATCACATAGATGGATTATGGTAAATTTAATTTTTCTATTCATCTATAGGAAACGTGTCTGGATGGATTCTCCCACGACGATGCGGACAAAAACCTTCGGGACAAGTTGAAAGAGGGGCAAATGGAAGTGGAACACATGTGCAGCAATGCATTAGCCATGATAAAGAACATGACAGACACAGATATAGCCAACTACGAGGCTAAAATGGGAATAACGAAAAATAGAAAACTCATGGCGGAGGAAGACGACAACAATAACGACGACGGAATCCAGTGGCCGGAGTGGCTGTCTGCCGGGGACCGACGACTGCTGCAATCGTCATCCGTGACGCCGAATGTGGTGGTGGCAGCGGACGGTAGTGGAAACTTTCGAACGGTGGCAGCAGCCGTGGCGGCGGCGCCGGTGAGAAGTAGTAAGAGATATGTGATAAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGAAGTGCCCAAGAAGAAGACGAATATTATGTTTATCGGAGATGGAAGACGGAATACCATAATTACCGGTAGCCGGAATGTGGTCGACGGCAGCACCACTTTCAACTCGGCAACCATGGGTAAGTTCGTTTTGGTTATCAATATTTTATCTTTTTGAAATTTTAGTTTTAATTTAAACAAAGTAGGAAAGATTTAATTTAGATATGTTTTTATAAATAACCAAATAAATTAAAATATTTACAAATTATAAGCAAAACTCATTAAAAACTTTTATTACTAACTATTAATGTCAATGATAAAATAACATAGATAACTATTAAATGTTTGTTATCGATAGAATTAAAATTTTTGTTATAGTTTATAAATATTTTATCAAATTTGCTATTTTTGACAATTTCTCTTTAATTGACCGTCCCAACACATGATTTAGTATGTCTTGGCATTTTGTTTTTTTGTTTTTCTTTTGTTGAAAACACCCTCCGAAGAATTATATGTATATATATGAGCAATAATTTTCTTTAGTAGGGCAATAGAATAAACCCTTTTTTGTAAGATAGTAAATGATTATAAAAATAATTATATATATATATATATATCTTTTTCACTTTTATTTATTTTGATAAAGGTACTTTCAAAAGATTTATTCTTATCTTTATATACATTGATTGTTTACTAAAAATTTTAAAATGTGACTATTTTGATATCTTTATATTGTAGTCACTTTTCCAAAGTACAAAATAAAGTTAAAAGTATATTATACCTATCTTCAGCTAAAGTTAAAAATTTTCGAGAAAAAGAAATTAAAACTACATAGAGATTGAAGATTAACATTTTGAAAATGCTACCGTAAAAATGAAAAGTCAGAAATATATAACCATACAACATTTAGAATGTAAGATATTGAGAAAAAAGTACTTTTAGCTTTTCCTTTAATCTTATGCTTTTTTAAGCCTCTCTTGAGAAGTAAAATAAATTTCATAAAAATAAATTTTACATTTCAAAGCATAGGAAAAATTAAGAAGAAGACAATTATCCTTCATTGAGCTTAATTAATCAATTAATTTTTCATCCTCTATAATCAAGGGACCAAATGAAGGGACAAAACTCCCTTACTTATTGAGGGATATTAAAAGAAAGATTACACTAAAAAGCAAGATTTGGAATGCCTATATTTAATTAATCACCTATACAGGAATAAAAGCAGCATTTAGTCGGTAGTTAGGCACAAGAAATAGAAAATGCCAAAATATTCTTTTTTTAAAAATTGATTTTTTTAATGCACCTATCTCCTCCCATTAAAAATATAATTGTAAAATTAAAAAATAAAAGAGATTTTACCAAATATTACGATTTATATTACAATTTATAGTAAATTCTTTTACTCATTGGCTATCCTAGATAAAATTCAAAATTTCGTTATAATTTATAAATATTTTAATTTATTTTATTATTTTAAAAAATGTCTCTTAGTAAGAGTGAGTTCATAAGGTTTGAAATCAATTTATTTTTCAATTTTCTAACTTTATCATAAAAATGATAGTAAATTCAATTTTAATTGTGACAAAATTAGATAGTTAGAATTAAGAAATCATATCGTAATTAAATTTTATTAAGAATAAATATTTAGAATTCATCAAATTTTATATATTTGAAAATGAGATTTAAAATTTTATACATTAGAAATATTTAAAATTCATTCTTTTTGTTTGGAGTTGTCCCCATCGTGGAAGAATCTCCTTGTTTCTCGTTGAAAGGCACCCCCACCTAATGCACGGTGTAGCGCACATGGGTAAAAATCACTTTTGCATTTAAATAACTATCGTTTCAAAAGTATTGTTGTTGGAAATAGGTAGACTTTTGTGGAACCAGCTCACAAGTCCAACCAATTTGAATATCTATCTAAAATAACTTTTTTCAACAACATAAAATTAGTATTAATTTTTTTTTTATTATGACTGAGTAAATCTATTATTCTTTTTAGTGGTTGTATGAGATTTTATACCTTCAAAATACTAAAATAATAATATATACTAAATTTATCTAATTAATTCACTTATGAGTTATGCCTTTCACAGCTGCGGTTGGTGAAGGATTTCTAGCGCGTGATATCACTTTCCAAAACACCGCCGGTCCATCAAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCAGATCTCTCAGCCTTTTACCAATGCGATATGTTAGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAATTCTACATCAACTGTCTCGTCTCTGGCACAGTCGATTTCATCTTCGGCAACGCCGCTGCCATTTTCCAAGACTGTGACATTCACGCACGCAAACCAAATTCCGGTCAGAAAAACATGGTCACGGCTCAAGGCCGGTCCGACCCGAACCAAAACACCGGTATTGTTATCCAAAAATCACGAATCGGAGCTACGTCGGATCTCCGCCCGGTTCAGAAGAGTTTCCCGACTTTTCTGGGAAGGCCGTGGAAGGAATACTCTAGAACGGTGATTATGCAGTGTACGATTAGCGACGTTATTGACCCGAAGGGATGGCATGAGTGGAGCGGTAGTTTTGCGCTTAACACGTTGTTTTACGGTGAGTATCAAAATACGGGAGCGGGGGCTTCGACTGGAGGGAGGGTGACGTGGAAGGGGTTTAGGGTAATTCGGAGTGCCACGGAGGCGGAAAGTTTTACCGCGGGTAAATTTATTGGCGGTGGGAGTTGGTTGAGCTCTACGGGCTTTCCTTTCTCTTTAGGATTATGAGGGAGGTAAATTTGATTTTTGTAATCGGCTCTTCATTTGTGGTAATTTTTTTTAGTTCTTTTTTTTTCTTCCTTATGTGTAATTTTCGAATTACAATTATTGGGTGATATTGTAAGTTTGGTAAAAAGAAAAATAATTGCCAATGAAGAGCCTGTGTTCACATGTGAAGAAATTAAATTTGGTGTATTGCTTACTTACTCGATGGTTACGTTTAACGTTTTCCATTCCAAAGATGACAACCAGCAGTAAATATGCCTAAT

mRNA sequence

CTCTATTGGGCATTTGGGTGTCTATATATTAAGCCCTTTACTCTTTTCCACCAACACTCACCATCATTTTCAAATCTCCGTCTCTTCATTCACAAAAATGAAAGAGACCGACGCCATGGAACAACCCATCGCCGCCGGCGCCGGAGTCTCAAACTCGAGCGACCTCCAAAAAACCCAAAAAAGTTCGAAGAAAAAGCTTCTAATCTTAACCCTTTGCTCCACTCTCCTCATCGTCGGTGCCGTCATTGGCATCGTTGCCGGAACAACAAAATCGTCGTCGAAATACTCCGGCGAGTCCCACGCCATTGTGAAGAGCACGTGCAGCAGCACGCTGTATCCGGACATGTGCTATTCTGCAATCTCGAGAAGTGAAGGAGCAATGTTGAAAGTGAAGAATCAAAAGGATGTGATTGAAATTTCGATTAATATTACGGTGAAAGCTGTGGAGGAAAATTACTTTAGAGTGAAGAAACTGTGTGGGTTGAAGAATATTAGTCATAGGGAGAGAATTGCTCTTCACGATTGTCTTGAGACGATTGATGAAACGCTTGACGAACTTCATAAAGCTATTGTGGATCTTAACGAATACCCAAATAAAAAGTCTCTTAACCAACATGCTGATGATCTCAAGACCTTACTTAGTTCCGCTATTACTAATCAGGAAACGTGTCTGGATGGATTCTCCCACGACGATGCGGACAAAAACCTTCGGGACAAGTTGAAAGAGGGGCAAATGGAAGTGGAACACATGTGCAGCAATGCATTAGCCATGATAAAGAACATGACAGACACAGATATAGCCAACTACGAGGCTAAAATGGGAATAACGAAAAATAGAAAACTCATGGCGGAGGAAGACGACAACAATAACGACGACGGAATCCAGTGGCCGGAGTGGCTGTCTGCCGGGGACCGACGACTGCTGCAATCGTCATCCGTGACGCCGAATGTGGTGGTGGCAGCGGACGGTAGTGGAAACTTTCGAACGGTGGCAGCAGCCGTGGCGGCGGCGCCGGTGAGAAGTAGTAAGAGATATGTGATAAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGAAGTGCCCAAGAAGAAGACGAATATTATGTTTATCGGAGATGGAAGACGGAATACCATAATTACCGGTAGCCGGAATGTGGTCGACGGCAGCACCACTTTCAACTCGGCAACCATGGCTGCGGTTGGTGAAGGATTTCTAGCGCGTGATATCACTTTCCAAAACACCGCCGGTCCATCAAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCAGATCTCTCAGCCTTTTACCAATGCGATATGTTAGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAATTCTACATCAACTGTCTCGTCTCTGGCACAGTCGATTTCATCTTCGGCAACGCCGCTGCCATTTTCCAAGACTGTGACATTCACGCACGCAAACCAAATTCCGGTCAGAAAAACATGGTCACGGCTCAAGGCCGGTCCGACCCGAACCAAAACACCGGTATTGTTATCCAAAAATCACGAATCGGAGCTACGTCGGATCTCCGCCCGGTTCAGAAGAGTTTCCCGACTTTTCTGGGAAGGCCGTGGAAGGAATACTCTAGAACGGTGATTATGCAGTGTACGATTAGCGACGTTATTGACCCGAAGGGATGGCATGAGTGGAGCGGTAGTTTTGCGCTTAACACGTTGTTTTACGGTGAGTATCAAAATACGGGAGCGGGGGCTTCGACTGGAGGGAGGGTGACGTGGAAGGGGTTTAGGGTAATTCGGAGTGCCACGGAGGCGGAAAGTTTTACCGCGGGTAAATTTATTGGCGGTGGGAGTTGGTTGAGCTCTACGGGCTTTCCTTTCTCTTTAGGATTATGAGGGAGGTAAATTTGATTTTTGTAATCGGCTCTTCATTTGTGGTAATTTTTTTTAGTTCTTTTTTTTTCTTCCTTATGTGTAATTTTCGAATTACAATTATTGGGTGATATTGTAAGTTTGGTAAAAAGAAAAATAATTGCCAATGAAGAGCCTGTGTTCACATGTGAAGAAATTAAATTTGGTGTATTGCTTACTTACTCGATGGTTACGTTTAACGTTTTCCATTCCAAAGATGACAACCAGCAGTAAATATGCCTAAT

Coding sequence (CDS)

ATGAAAGAGACCGACGCCATGGAACAACCCATCGCCGCCGGCGCCGGAGTCTCAAACTCGAGCGACCTCCAAAAAACCCAAAAAAGTTCGAAGAAAAAGCTTCTAATCTTAACCCTTTGCTCCACTCTCCTCATCGTCGGTGCCGTCATTGGCATCGTTGCCGGAACAACAAAATCGTCGTCGAAATACTCCGGCGAGTCCCACGCCATTGTGAAGAGCACGTGCAGCAGCACGCTGTATCCGGACATGTGCTATTCTGCAATCTCGAGAAGTGAAGGAGCAATGTTGAAAGTGAAGAATCAAAAGGATGTGATTGAAATTTCGATTAATATTACGGTGAAAGCTGTGGAGGAAAATTACTTTAGAGTGAAGAAACTGTGTGGGTTGAAGAATATTAGTCATAGGGAGAGAATTGCTCTTCACGATTGTCTTGAGACGATTGATGAAACGCTTGACGAACTTCATAAAGCTATTGTGGATCTTAACGAATACCCAAATAAAAAGTCTCTTAACCAACATGCTGATGATCTCAAGACCTTACTTAGTTCCGCTATTACTAATCAGGAAACGTGTCTGGATGGATTCTCCCACGACGATGCGGACAAAAACCTTCGGGACAAGTTGAAAGAGGGGCAAATGGAAGTGGAACACATGTGCAGCAATGCATTAGCCATGATAAAGAACATGACAGACACAGATATAGCCAACTACGAGGCTAAAATGGGAATAACGAAAAATAGAAAACTCATGGCGGAGGAAGACGACAACAATAACGACGACGGAATCCAGTGGCCGGAGTGGCTGTCTGCCGGGGACCGACGACTGCTGCAATCGTCATCCGTGACGCCGAATGTGGTGGTGGCAGCGGACGGTAGTGGAAACTTTCGAACGGTGGCAGCAGCCGTGGCGGCGGCGCCGGTGAGAAGTAGTAAGAGATATGTGATAAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGAAGTGCCCAAGAAGAAGACGAATATTATGTTTATCGGAGATGGAAGACGGAATACCATAATTACCGGTAGCCGGAATGTGGTCGACGGCAGCACCACTTTCAACTCGGCAACCATGGCTGCGGTTGGTGAAGGATTTCTAGCGCGTGATATCACTTTCCAAAACACCGCCGGTCCATCAAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCAGATCTCTCAGCCTTTTACCAATGCGATATGTTAGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAATTCTACATCAACTGTCTCGTCTCTGGCACAGTCGATTTCATCTTCGGCAACGCCGCTGCCATTTTCCAAGACTGTGACATTCACGCACGCAAACCAAATTCCGGTCAGAAAAACATGGTCACGGCTCAAGGCCGGTCCGACCCGAACCAAAACACCGGTATTGTTATCCAAAAATCACGAATCGGAGCTACGTCGGATCTCCGCCCGGTTCAGAAGAGTTTCCCGACTTTTCTGGGAAGGCCGTGGAAGGAATACTCTAGAACGGTGATTATGCAGTGTACGATTAGCGACGTTATTGACCCGAAGGGATGGCATGAGTGGAGCGGTAGTTTTGCGCTTAACACGTTGTTTTACGGTGAGTATCAAAATACGGGAGCGGGGGCTTCGACTGGAGGGAGGGTGACGTGGAAGGGGTTTAGGGTAATTCGGAGTGCCACGGAGGCGGAAAGTTTTACCGCGGGTAAATTTATTGGCGGTGGGAGTTGGTTGAGCTCTACGGGCTTTCCTTTCTCTTTAGGATTATGA

Protein sequence

MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL*
Homology
BLAST of CsGy7G020340 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 2.9e-241
Identity = 430/570 (75.44%), Postives = 485/570 (85.09%), Query Frame = 0

Query: 31  KKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGES-----HAIVKSTCSSTLYPDMCY 90
           KKK L L L +TLL+V AVIGIVAG   +S K SG++     HAI+KS+CSST YPD+C+
Sbjct: 26  KKKKLFLALFATLLVVAAVIGIVAGV--NSRKNSGDNGNEPHHAILKSSCSSTRYPDLCF 85

Query: 91  SAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNISHRERIALHDCLE 150
           SAI+    A  KV +QKDVIE+S+NIT  AVE NYF ++KL    N++ RE++ALHDCLE
Sbjct: 86  SAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLE 145

Query: 151 TIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADKNLR 210
           TIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLDGFSHDDA+K++R
Sbjct: 146 TIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVR 205

Query: 211 DKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNNDDGIQWP 270
           D L +GQ+ VE MCSNALAMIKNMTDTD+      M  + NRKL+   ++ +  DG  WP
Sbjct: 206 DALSDGQVHVEKMCSNALAMIKNMTDTDM----MIMRTSNNRKLI---EETSTVDG--WP 265

Query: 271 EWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIKAGVYREN 330
            WLS GDRRLLQSSSVTPNVVVAADGSGNF+TVAA+VAAAP   +KRY+IRIKAGVYREN
Sbjct: 266 AWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYREN 325

Query: 331 VEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDITFQNTAGP 390
           VEV KK  NIMFIGDGR  TIITGSRNVVDGSTTF SAT+A VGEGFLARDITFQNTAGP
Sbjct: 326 VEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGP 385

Query: 391 SKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAIFQD 450
           SKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL++GTVDFIFGNAAA+ Q+
Sbjct: 386 SKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQN 445

Query: 451 CDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRPWKE 510
           CDIHARKPNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ SFPT+LGRPWKE
Sbjct: 446 CDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKE 505

Query: 511 YSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWKGFRVIRS 570
           YSRTVIMQ +I+DVI P GWHEW G+FALNTLFYGE+QN GAGA T GRV WKGFRVI S
Sbjct: 506 YSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITS 565

Query: 571 ATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
           ATEA++FT G FI G SWL STGFPFSLGL
Sbjct: 566 ATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy7G020340 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.2e-240
Identity = 431/582 (74.05%), Postives = 488/582 (83.85%), Query Frame = 0

Query: 19  NSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGES-----HAIVKS 78
           +SS+   +    KKK L L L +TLL+V AVIGIVAG   +S K SG++     HAI+KS
Sbjct: 14  SSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGV--NSRKNSGDNGNEPHHAILKS 73

Query: 79  TCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNIS 138
           +CSST YPD+C+SAI+    A  KV +QKDVIE+S+NIT  AVE NYF ++KL    N++
Sbjct: 74  SCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLT 133

Query: 139 HRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLD 198
            RE++ALHDCLETIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLD
Sbjct: 134 KREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLD 193

Query: 199 GFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEE 258
           GFSHDDA+K++RD L +GQ+ VE MCSNALAMIKNMTDTD+      M  + NRKL    
Sbjct: 194 GFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDM----MIMRTSNNRKL---T 253

Query: 259 DDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRY 318
           ++ +  DG  WP WLS GDRRLLQSSSVTPN VVAADGSGNF+TVAAAVAAAP   +KRY
Sbjct: 254 EETSTVDG--WPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRY 313

Query: 319 VIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFL 378
           +IRIKAGVYRENVEV KK  NIMFIGDGR  TIITGSRNVVDGSTTF SAT A VGEGFL
Sbjct: 314 IIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFL 373

Query: 379 ARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVD 438
           ARDITFQNTAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL++GTVD
Sbjct: 374 ARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVD 433

Query: 439 FIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQK 498
           FIFGNAAA+ Q+CDIHARKPNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ 
Sbjct: 434 FIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQG 493

Query: 499 SFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGG 558
           SFPT+LGRPWKEYSRTVIMQ +I+D+I P GWHEW G+FALNTLFYGE+QN+GAGA T G
Sbjct: 494 SFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSG 553

Query: 559 RVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
           RV WKGFRVI SATEA++FT G FI G SWL STGFPFSLGL
Sbjct: 554 RVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy7G020340 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 7.4e-229
Identity = 408/576 (70.83%), Postives = 473/576 (82.12%), Query Frame = 0

Query: 31  KKKLLILTLCSTLLIVGAVIGIVAGTTKSSSK----------YSGESHAIVKSTCSSTLY 90
           +KK + L + +++L+V AVIG+VAG  KS SK           S  +HAIVKS CS+TL+
Sbjct: 17  RKKKIYLAIVASVLLVAAVIGVVAG-VKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 76

Query: 91  PDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGL-KNISHRERIA 150
           P++CYSAI        KV +QKDVIE+S+NITVKAV  NY+ VK+L    K ++ RE++A
Sbjct: 77  PELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 136

Query: 151 LHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDD 210
           LHDCLET+DETLDELH A+ DL  YPNKKSL +H +DLKTL+SSAITNQETCLDGFSHD+
Sbjct: 137 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 196

Query: 211 ADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNND 270
           ADK +R  L +GQ  VE MCSNALAMI NMTDTDIAN         NRKL+        +
Sbjct: 197 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLV--------E 256

Query: 271 DGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIKA 330
           D  +WPEWLSAGDRRLLQSS+VTP+VVVAADGSG+++TV+ AV  AP +SSKRYVIRIKA
Sbjct: 257 DNGEWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKA 316

Query: 331 GVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDITF 390
           GVYRENV+VPKKKTNIMF+GDG+ NTIIT SRNV DGSTTF+SAT+  V    LARDITF
Sbjct: 317 GVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITF 376

Query: 391 QNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNA 450
           QNTAG SKHQAVAL VG+DLSAFY+CDMLAYQDTLYVHSNRQF++ CLV+GTVDFIFGN 
Sbjct: 377 QNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNG 436

Query: 451 AAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFL 510
           AA+FQDCDIHAR+P SGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDLRPVQKSFPT+L
Sbjct: 437 AAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYL 496

Query: 511 GRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWKG 570
           GRPWKEYSRTVIMQ +I+DVI P GWHEW+G+FAL+TLFYGEY NTGAGA T GRV WKG
Sbjct: 497 GRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKG 556

Query: 571 FRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
            +VI S+TEA+++T G+FI GGSWLSSTGFPFSLGL
Sbjct: 557 HKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of CsGy7G020340 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 788.5 bits (2035), Expect = 5.3e-227
Identity = 405/584 (69.35%), Postives = 469/584 (80.31%), Query Frame = 0

Query: 25  KTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSK--YSGESHAIVKSTCSSTLYPD 84
           K      KKL++L+    LL V AV GI AG +K++ K   S  SHA+++S+CSST YP+
Sbjct: 11  KDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPE 70

Query: 85  MCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLC-GLKNISHRERIALH 144
           +C SA+  + G  +++ +QKDVIE S+N+T+ AVE NYF VKKL    K ++ RE+ ALH
Sbjct: 71  LCISAVVTAGG--VELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 130

Query: 145 DCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDAD 204
           DCLETIDETLDELH+ + DL+ YP KK+L +HA DLKTL+SSAITNQETCLDGFSHDDAD
Sbjct: 131 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 190

Query: 205 KNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNND-- 264
           K +R  L +GQ+ VEHMCSNALAMIKNMTDTDIAN+E K  IT N + + EE+       
Sbjct: 191 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 250

Query: 265 --------DGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSK 324
                   D   WP WLSAGDRRLLQ S V  +  VAADGSG F+TVAAAVAAAP  S+K
Sbjct: 251 DIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNK 310

Query: 325 RYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEG 384
           RYVI IKAGVYRENVEV KKK NIMF+GDGR  TIITGSRNVVDGSTTF+SAT+AAVGE 
Sbjct: 311 RYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGER 370

Query: 385 FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGT 444
           FLARDITFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL++GT
Sbjct: 371 FLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGT 430

Query: 445 VDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 504
           VDFIFGNAA + QDCDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V
Sbjct: 431 VDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSV 490

Query: 505 QKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGAST 564
           + SFPT+LGRPWKEYS+TVIMQ  ISDVI P+GW EW+G+FALNTL Y EY NTGAGA T
Sbjct: 491 KGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGT 550

Query: 565 GGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
             RV W+GF+VI +A EA+ +TAG+FIGGG WLSSTGFPFSLGL
Sbjct: 551 ANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy7G020340 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 763.5 bits (1970), Expect = 1.8e-219
Identity = 393/577 (68.11%), Postives = 465/577 (80.59%), Query Frame = 0

Query: 30  SKKKLLILTLCSTLLIVGAVIGIVAGTTKSS-----SKYSGESHAIVKSTCSSTLYPDMC 89
           + KKL++ +    LL++ +++GI A TT  +     +  S  SHAI+KS CSSTLYP++C
Sbjct: 16  NNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELC 75

Query: 90  YSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCG-LKNISHRERIALHDC 149
           +SA++ + G   ++ +QK+VIE S+N+T KAV+ NYF VKKL    K ++ RE  ALHDC
Sbjct: 76  FSAVAATGGK--ELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 135

Query: 150 LETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADKN 209
           LETIDETLDELH A+ DL++YP +KSL +HADDLKTL+SSAITNQ TCLDGFS+DDAD+ 
Sbjct: 136 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 195

Query: 210 LRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK-----MGITKNRKLMAEEDDNNN 269
           +R  L +GQ+ VEHMCSNALAMIKNMT+TDIAN+E +          NRKL     D ++
Sbjct: 196 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 255

Query: 270 DDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIK 329
           D    WP+WLS GDRRLLQ S++  +  VA DGSG+F TVAAAVAAAP +S+KR+VI IK
Sbjct: 256 DG---WPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIK 315

Query: 330 AGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDIT 389
           AGVYRENVEV KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SAT+AAVGE FLARDIT
Sbjct: 316 AGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDIT 375

Query: 390 FQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGN 449
           FQNTAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C ++GTVDFIFGN
Sbjct: 376 FQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGN 435

Query: 450 AAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTF 509
           AAA+ QDCDI+AR+PNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL  V+ +FPT+
Sbjct: 436 AAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTY 495

Query: 510 LGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWK 569
           LGRPWKEYSRTVIMQ  ISDVI P+GWHEWSGSFAL+TL Y EY N G GA T  RV WK
Sbjct: 496 LGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWK 555

Query: 570 GFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
           G++VI S TEA+ FTAG+FIGGG WL+STGFPFSL L
Sbjct: 556 GYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy7G020340 vs. NCBI nr
Match: XP_004141734.1 (pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cucumis sativus])

HSP 1 Score: 1164 bits (3011), Expect = 0.0
Identity = 594/595 (99.83%), Postives = 595/595 (100.00%), Query Frame = 0

Query: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSS 60
           MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLI+TLCSTLLIVGAVIGIVAGTTKSS
Sbjct: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKSS 60

Query: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120
           SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY
Sbjct: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120

Query: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180
           FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL
Sbjct: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180

Query: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240
           LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK
Sbjct: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240

Query: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300
           MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA
Sbjct: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300

Query: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360
           AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF
Sbjct: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360

Query: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420
           NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR
Sbjct: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420

Query: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480
           QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS
Sbjct: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480

Query: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540
           RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG
Sbjct: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540

Query: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL
Sbjct: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of CsGy7G020340 vs. NCBI nr
Match: KAA0059355.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK03971.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 566/595 (95.13%), Postives = 582/595 (97.82%), Query Frame = 0

Query: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSS 60
           MKETDAMEQPIAAGAG+SNSSDL+K +K+SKK LLILTL STLLIVGAVIGIVAGTTKSS
Sbjct: 1   MKETDAMEQPIAAGAGISNSSDLKKNKKASKK-LLILTLSSTLLIVGAVIGIVAGTTKSS 60

Query: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120
            K SG+SHAIVKSTCSSTLYPDMCYSAIS+SEGAMLKVK+QKDVIE+SINITVKAVEENY
Sbjct: 61  -KDSGKSHAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENY 120

Query: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180
           FRVKKLCGLKNISHRE+IALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL
Sbjct: 121 FRVKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180

Query: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240
           LSSAITNQETCLDGFSHD+ADK LRDKLK GQMEVEHMCSNALAMIKNMTDTDIANYEAK
Sbjct: 181 LSSAITNQETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240

Query: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300
           MGITKNRKLMAEE+DNNN+D IQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA
Sbjct: 241 MGITKNRKLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300

Query: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360
           AVAAAP +SSKRYVIRIKAGVYRENVEVPKKKTNIMF+GDGRRNTIITGSRNVVDGSTTF
Sbjct: 301 AVAAAPEKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTF 360

Query: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420
            SAT+AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR
Sbjct: 361 KSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420

Query: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480
           QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS
Sbjct: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480

Query: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540
           RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQC+ISDVIDPKGWHEWSGSFALNTLFYG
Sbjct: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYG 540

Query: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           EYQNTG GASTGGRVTWKGFRVIRSATEAE FTAGKFIGGGSWL STGFPFSLGL
Sbjct: 541 EYQNTGPGASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 593

BLAST of CsGy7G020340 vs. NCBI nr
Match: XP_016902864.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 494/513 (96.30%), Postives = 505/513 (98.44%), Query Frame = 0

Query: 83  MCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNISHRERIALHD 142
           MCYSAIS+SEGAMLKVK+QKDVIE+SINITVKAVEENYFRVKKLCGLKNISHRE+IALHD
Sbjct: 1   MCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENYFRVKKLCGLKNISHREKIALHD 60

Query: 143 CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADK 202
           CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHD+ADK
Sbjct: 61  CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDNADK 120

Query: 203 NLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNNDDGI 262
            LRDKLK GQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEE+DNNN+D I
Sbjct: 121 TLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEEDNNNNDEI 180

Query: 263 QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIKAGVY 322
           QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP +SSKRYVIRIKAGVY
Sbjct: 181 QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPEKSSKRYVIRIKAGVY 240

Query: 323 RENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDITFQNT 382
           RENVEVPKKKTNIMF+GDGRRNTIITGSRNVVDGSTTF SAT+AAVGEGFLARDITFQNT
Sbjct: 241 RENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVGEGFLARDITFQNT 300

Query: 383 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI 442
           AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI
Sbjct: 301 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI 360

Query: 443 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP 502
           FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP
Sbjct: 361 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP 420

Query: 503 WKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWKGFRV 562
           WKEYSRTVIMQC+ISDVIDPKGWHEWSGSFALNTLFYGEYQNTG GASTGGRVTWKGFRV
Sbjct: 421 WKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYGEYQNTGPGASTGGRVTWKGFRV 480

Query: 563 IRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           IRSATEAE FTAGKFIGGGSWL STGFPFSLGL
Sbjct: 481 IRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 513

BLAST of CsGy7G020340 vs. NCBI nr
Match: XP_038897967.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 973 bits (2516), Expect = 0.0
Identity = 502/595 (84.37%), Postives = 535/595 (89.92%), Query Frame = 0

Query: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSS 60
           MK +D ME+PIAAG  +SNS +  +  K+  +KLLIL + STLL+VGA +GIVAG T + 
Sbjct: 1   MKHSDTMEKPIAAG--ISNSGNHPQNPKNKSRKLLILAISSTLLLVGAAVGIVAGVT-TK 60

Query: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120
           SK SG+SHAIVKSTCSSTLYPD+C+S IS+SEGAM KV NQKDVIE+SINITVKAVEENY
Sbjct: 61  SKNSGKSHAIVKSTCSSTLYPDLCFSTISKSEGAMEKVGNQKDVIELSINITVKAVEENY 120

Query: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180
           F+VKKLCGLKNISHRE+IALHDCLETIDETLDELHKAIVDL+EYPNKKSLNQHADDLKTL
Sbjct: 121 FKVKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLNQHADDLKTL 180

Query: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240
           LSSAITNQ TCLDGFSHD   +    K K GQ+EVEHMCSNALAMIKNMTDTDIANYEAK
Sbjct: 181 LSSAITNQGTCLDGFSHDRGRQKPAGKAKRGQVEVEHMCSNALAMIKNMTDTDIANYEAK 240

Query: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300
           M   K        +     +  QWPEWLSAGDRRLLQSSSV P+VVVAADGSGNFRTVAA
Sbjct: 241 MEQLKTENSW-RRNRTTTTNSHQWPEWLSAGDRRLLQSSSVNPDVVVAADGSGNFRTVAA 300

Query: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360
           AVAAAPV+SSKRYVIRIKAGVYRENVEVPKKKTNIMF+GDGR NTIITGSRNVVDGSTTF
Sbjct: 301 AVAAAPVKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTF 360

Query: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420
            SAT+AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR
Sbjct: 361 KSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420

Query: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480
           QFYINCL+SGTVDFIFGNAA IFQDCDIHAR+PNSGQKNMVTAQ RSDPNQNTGIVIQKS
Sbjct: 421 QFYINCLISGTVDFIFGNAAVIFQDCDIHARRPNSGQKNMVTAQSRSDPNQNTGIVIQKS 480

Query: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540
           RIGATSDLR VQK+FPTFLGRPWKEYSRTVI+Q TISDVIDPKGWHEWSG+F LNTLFYG
Sbjct: 481 RIGATSDLRSVQKNFPTFLGRPWKEYSRTVIIQSTISDVIDPKGWHEWSGNFGLNTLFYG 540

Query: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           EYQNTG GASTGGRVTWKGFRVI+SATEAE FTAGKFIGGGSWL STGFPFSLGL
Sbjct: 541 EYQNTGPGASTGGRVTWKGFRVIQSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 591

BLAST of CsGy7G020340 vs. NCBI nr
Match: XP_023549002.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 500/590 (84.75%), Postives = 538/590 (91.19%), Query Frame = 0

Query: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66
           MEQ IAAG  +S+S +     K+++KKL+IL + STLL+VG V G+V+       +   +
Sbjct: 1   MEQIIAAG--ISSSGNQLPKPKNNRKKLIILAVSSTLLLVGIVAGVVSSRNGGGEEEEAK 60

Query: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126
           SHAIVKSTCSSTLYPD+C+S+I RS+GAM +V N+KDVIE+SINITVKAVEENYFRV KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILRSDGAMKRVGNKKDVIEVSINITVKAVEENYFRVLKL 120

Query: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186
           C LK+ISHRE+IALHDCLETIDETLDELHKA VDL+EYPNKKSLNQ ADDLKTLLSSAIT
Sbjct: 121 CELKDISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQRADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITK- 246
           NQETCLDGFSHD+ADKNLRDKLKEGQ+EVE MCSNALAMIKNMTDTDIANYEAKMG T  
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTTTA 240

Query: 247 NRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAA 306
           NRKL  E    NND+ IQWPEWLSAGDRRLLQSSSV  +VVVAADGSGNF+TVAAAVAAA
Sbjct: 241 NRKLKEE----NNDEQIQWPEWLSAGDRRLLQSSSVKADVVVAADGSGNFKTVAAAVAAA 300

Query: 307 PVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATM 366
           PV+SSKRYVIRIKAGVYRENV+VPKKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+
Sbjct: 301 PVKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATV 360

Query: 367 AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 426
           AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN
Sbjct: 361 AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 420

Query: 427 CLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 486
           CL+SGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT
Sbjct: 421 CLISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 480

Query: 487 SDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNT 546
           SDLR VQKSFPTFLGRPWKEYSRTVIMQ +ISDVID KGWHEWSG+FALNTLFYGEYQNT
Sbjct: 481 SDLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNT 540

Query: 547 GAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           G GAST GRVTWKGFRVIRSATEAE FT GKFIGGGSWL +TGFPFSLGL
Sbjct: 541 GPGASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 584

BLAST of CsGy7G020340 vs. ExPASy TrEMBL
Match: A0A0A0KAM6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G447990 PE=3 SV=1)

HSP 1 Score: 1164 bits (3011), Expect = 0.0
Identity = 594/595 (99.83%), Postives = 595/595 (100.00%), Query Frame = 0

Query: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSS 60
           MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLI+TLCSTLLIVGAVIGIVAGTTKSS
Sbjct: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKSS 60

Query: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120
           SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY
Sbjct: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120

Query: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180
           FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL
Sbjct: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180

Query: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240
           LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK
Sbjct: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240

Query: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300
           MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA
Sbjct: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300

Query: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360
           AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF
Sbjct: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360

Query: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420
           NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR
Sbjct: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420

Query: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480
           QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS
Sbjct: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480

Query: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540
           RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG
Sbjct: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540

Query: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL
Sbjct: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of CsGy7G020340 vs. ExPASy TrEMBL
Match: A0A5A7V0J0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001680 PE=3 SV=1)

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 566/595 (95.13%), Postives = 582/595 (97.82%), Query Frame = 0

Query: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSS 60
           MKETDAMEQPIAAGAG+SNSSDL+K +K+SKK LLILTL STLLIVGAVIGIVAGTTKSS
Sbjct: 1   MKETDAMEQPIAAGAGISNSSDLKKNKKASKK-LLILTLSSTLLIVGAVIGIVAGTTKSS 60

Query: 61  SKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENY 120
            K SG+SHAIVKSTCSSTLYPDMCYSAIS+SEGAMLKVK+QKDVIE+SINITVKAVEENY
Sbjct: 61  -KDSGKSHAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENY 120

Query: 121 FRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180
           FRVKKLCGLKNISHRE+IALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL
Sbjct: 121 FRVKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTL 180

Query: 181 LSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240
           LSSAITNQETCLDGFSHD+ADK LRDKLK GQMEVEHMCSNALAMIKNMTDTDIANYEAK
Sbjct: 181 LSSAITNQETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAK 240

Query: 241 MGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300
           MGITKNRKLMAEE+DNNN+D IQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA
Sbjct: 241 MGITKNRKLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAA 300

Query: 301 AVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTF 360
           AVAAAP +SSKRYVIRIKAGVYRENVEVPKKKTNIMF+GDGRRNTIITGSRNVVDGSTTF
Sbjct: 301 AVAAAPEKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTF 360

Query: 361 NSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420
            SAT+AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR
Sbjct: 361 KSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNR 420

Query: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480
           QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS
Sbjct: 421 QFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKS 480

Query: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYG 540
           RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQC+ISDVIDPKGWHEWSGSFALNTLFYG
Sbjct: 481 RIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYG 540

Query: 541 EYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           EYQNTG GASTGGRVTWKGFRVIRSATEAE FTAGKFIGGGSWL STGFPFSLGL
Sbjct: 541 EYQNTGPGASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 593

BLAST of CsGy7G020340 vs. ExPASy TrEMBL
Match: A0A1S4E3R0 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500802 PE=3 SV=1)

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 494/513 (96.30%), Postives = 505/513 (98.44%), Query Frame = 0

Query: 83  MCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNISHRERIALHD 142
           MCYSAIS+SEGAMLKVK+QKDVIE+SINITVKAVEENYFRVKKLCGLKNISHRE+IALHD
Sbjct: 1   MCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENYFRVKKLCGLKNISHREKIALHD 60

Query: 143 CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADK 202
           CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHD+ADK
Sbjct: 61  CLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDNADK 120

Query: 203 NLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNNDDGI 262
            LRDKLK GQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEE+DNNN+D I
Sbjct: 121 TLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEEDNNNNDEI 180

Query: 263 QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIKAGVY 322
           QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP +SSKRYVIRIKAGVY
Sbjct: 181 QWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPEKSSKRYVIRIKAGVY 240

Query: 323 RENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDITFQNT 382
           RENVEVPKKKTNIMF+GDGRRNTIITGSRNVVDGSTTF SAT+AAVGEGFLARDITFQNT
Sbjct: 241 RENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVGEGFLARDITFQNT 300

Query: 383 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI 442
           AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI
Sbjct: 301 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAI 360

Query: 443 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP 502
           FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP
Sbjct: 361 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRP 420

Query: 503 WKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWKGFRV 562
           WKEYSRTVIMQC+ISDVIDPKGWHEWSGSFALNTLFYGEYQNTG GASTGGRVTWKGFRV
Sbjct: 421 WKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYGEYQNTGPGASTGGRVTWKGFRV 480

Query: 563 IRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
           IRSATEAE FTAGKFIGGGSWL STGFPFSLGL
Sbjct: 481 IRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 513

BLAST of CsGy7G020340 vs. ExPASy TrEMBL
Match: A0A6J1GQR0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456237 PE=3 SV=1)

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 498/589 (84.55%), Postives = 540/589 (91.68%), Query Frame = 0

Query: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66
           MEQPIAAG  +S+S +     K+++KKL+IL + STLL+VG V G+V+   ++      +
Sbjct: 1   MEQPIAAG--ISSSGNHLPKPKNNRKKLIILAVSSTLLLVGIVAGVVSS--RNGGGEEAK 60

Query: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126
           SHAIVKSTCSSTLYPD+C+S+I +S+GAM +V ++KDVIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186
             LKNISHRE+IALHDCLETIDETLDELHKA VDL+EYPNKKSLNQHADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKN 246
           NQETCLDGFSHD+ADKNLRDKLKEGQ+EVE MCSNALAMIKNMTDTDIANYEAKMG T N
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTTAN 240

Query: 247 RKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP 306
           RKL  E     ND+ IQWPEWLSAGDRRLLQSSSV P+VVVAADGSGNF+TVAAAVAAAP
Sbjct: 241 RKLKEEI----NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300

Query: 307 VRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMA 366
           V+SSKR+VIRIKAGVYRENV+VPKKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+A
Sbjct: 301 VKSSKRFVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 367 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 426
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 427 LVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 486
           L+SGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 487 DLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTG 546
           DLR VQKSFPTFLGRPWKEYSRTVIMQ +ISDVID KGWHEWSG+FALNTLFYGEYQNTG
Sbjct: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540

Query: 547 AGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
            GAST GRVTWKGFRVIRSATEAE FT GKFIGGGSWL +TGFPFSLGL
Sbjct: 541 PGASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of CsGy7G020340 vs. ExPASy TrEMBL
Match: A0A6J1JSY6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488604 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 496/589 (84.21%), Postives = 536/589 (91.00%), Query Frame = 0

Query: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66
           MEQPIAAG  +S+S +     K ++KKL+IL + STLL+VG V G+V+   +++     +
Sbjct: 1   MEQPIAAG--ISSSGNHLPKPKYNRKKLIILAVSSTLLLVGIVAGVVSS--RNAGGEESK 60

Query: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126
           SHAIVKSTCSSTLYPD+C+S+I +++ AM KV N+KDVIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186
             LKNISHRE+IALHDCLETIDETLDELHKA VDL+EYPNKKSL Q ADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKN 246
           NQETCLDGFSHD+ADKNLRDKLKEGQ+EVE MCSNALAMIKNMTDTDIANYE KMG   N
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAAN 240

Query: 247 RKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP 306
           RKL  E    NND+ IQWPEWLSAGDRRLLQSSSV P+VVVAADGSGNF+TVAAAVAAAP
Sbjct: 241 RKLKEE----NNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300

Query: 307 VRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMA 366
           V+SSKRYVIRIKAGVYRENV+V KKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+A
Sbjct: 301 VKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 367 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 426
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 427 LVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 486
           L+SGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 487 DLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTG 546
           DLR VQKSFPTFLGRPWKEYSRTVIMQ +ISDVID KGWHEWSG+FALNTLFYGEYQNTG
Sbjct: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540

Query: 547 AGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595
            GASTGGRVTWKGFRVIRSATEAE FT GKFIGGGSWL +TGFPFSLGL
Sbjct: 541 PGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of CsGy7G020340 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 788.5 bits (2035), Expect = 3.8e-228
Identity = 405/584 (69.35%), Postives = 469/584 (80.31%), Query Frame = 0

Query: 25  KTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSK--YSGESHAIVKSTCSSTLYPD 84
           K      KKL++L+    LL V AV GI AG +K++ K   S  SHA+++S+CSST YP+
Sbjct: 11  KDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPE 70

Query: 85  MCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLC-GLKNISHRERIALH 144
           +C SA+  + G  +++ +QKDVIE S+N+T+ AVE NYF VKKL    K ++ RE+ ALH
Sbjct: 71  LCISAVVTAGG--VELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 130

Query: 145 DCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDAD 204
           DCLETIDETLDELH+ + DL+ YP KK+L +HA DLKTL+SSAITNQETCLDGFSHDDAD
Sbjct: 131 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 190

Query: 205 KNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEDDNNND-- 264
           K +R  L +GQ+ VEHMCSNALAMIKNMTDTDIAN+E K  IT N + + EE+       
Sbjct: 191 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 250

Query: 265 --------DGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSK 324
                   D   WP WLSAGDRRLLQ S V  +  VAADGSG F+TVAAAVAAAP  S+K
Sbjct: 251 DIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNK 310

Query: 325 RYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEG 384
           RYVI IKAGVYRENVEV KKK NIMF+GDGR  TIITGSRNVVDGSTTF+SAT+AAVGE 
Sbjct: 311 RYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGER 370

Query: 385 FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGT 444
           FLARDITFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL++GT
Sbjct: 371 FLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGT 430

Query: 445 VDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 504
           VDFIFGNAA + QDCDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V
Sbjct: 431 VDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSV 490

Query: 505 QKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGAST 564
           + SFPT+LGRPWKEYS+TVIMQ  ISDVI P+GW EW+G+FALNTL Y EY NTGAGA T
Sbjct: 491 KGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGT 550

Query: 565 GGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
             RV W+GF+VI +A EA+ +TAG+FIGGG WLSSTGFPFSLGL
Sbjct: 551 ANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy7G020340 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 763.5 bits (1970), Expect = 1.3e-220
Identity = 393/577 (68.11%), Postives = 465/577 (80.59%), Query Frame = 0

Query: 30  SKKKLLILTLCSTLLIVGAVIGIVAGTTKSS-----SKYSGESHAIVKSTCSSTLYPDMC 89
           + KKL++ +    LL++ +++GI A TT  +     +  S  SHAI+KS CSSTLYP++C
Sbjct: 16  NNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELC 75

Query: 90  YSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCG-LKNISHRERIALHDC 149
           +SA++ + G   ++ +QK+VIE S+N+T KAV+ NYF VKKL    K ++ RE  ALHDC
Sbjct: 76  FSAVAATGGK--ELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 135

Query: 150 LETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDDADKN 209
           LETIDETLDELH A+ DL++YP +KSL +HADDLKTL+SSAITNQ TCLDGFS+DDAD+ 
Sbjct: 136 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 195

Query: 210 LRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAK-----MGITKNRKLMAEEDDNNN 269
           +R  L +GQ+ VEHMCSNALAMIKNMT+TDIAN+E +          NRKL     D ++
Sbjct: 196 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 255

Query: 270 DDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPVRSSKRYVIRIK 329
           D    WP+WLS GDRRLLQ S++  +  VA DGSG+F TVAAAVAAAP +S+KR+VI IK
Sbjct: 256 DG---WPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIK 315

Query: 330 AGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVGEGFLARDIT 389
           AGVYRENVEV KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SAT+AAVGE FLARDIT
Sbjct: 316 AGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDIT 375

Query: 390 FQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGN 449
           FQNTAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C ++GTVDFIFGN
Sbjct: 376 FQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGN 435

Query: 450 AAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTF 509
           AAA+ QDCDI+AR+PNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL  V+ +FPT+
Sbjct: 436 AAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTY 495

Query: 510 LGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGASTGGRVTWK 569
           LGRPWKEYSRTVIMQ  ISDVI P+GWHEWSGSFAL+TL Y EY N G GA T  RV WK
Sbjct: 496 LGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWK 555

Query: 570 GFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
           G++VI S TEA+ FTAG+FIGGG WL+STGFPFSL L
Sbjct: 556 GYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy7G020340 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 449.5 bits (1155), Expect = 4.2e-126
Identity = 254/590 (43.05%), Postives = 362/590 (61.36%), Query Frame = 0

Query: 20  SSDLQKTQKSSKKKLLILTLCSTLLIVGAV-IGIVAG-----TTKSSSKYSGESHAIVKS 79
           ++ L + QK +K KL++ TL   +L+VG V  GI AG     + K+  K + +    +  
Sbjct: 20  ATSLPELQKKTKTKLILFTL--AVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISR 79

Query: 80  TCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNIS 139
           TCS +LYP++C   +    G++    ++ ++I IS N T++   +  +    +     + 
Sbjct: 80  TCSKSLYPNLCIDTLLDFPGSL--TADENELIHISFNATLQKFSKALYTSSTIT-YTQMP 139

Query: 140 HRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLD 199
            R R A   CLE +D+++D L +A+  +      +S      D+ T LSSA+TN +TC D
Sbjct: 140 PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES----HSDVMTWLSSAMTNHDTCTD 199

Query: 200 GFSH-DDADKNLRDKLKEGQMEVEHMCSNALAM----IKNMTDTDIANYEAKMGITKNRK 259
           GF   +     ++D++     ++  M SN LA+    +K+++   + N         NRK
Sbjct: 200 GFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN---------NRK 259

Query: 260 LMAEEDDNNNDDGIQWPEWLSAGDRRLL--QSSSVTPNVVVAADGSGNFRTVAAAVAAAP 319
           L+  E+        + P WL   DR LL   +S++  ++ V+ DGSG F+T+A A+  AP
Sbjct: 260 LLGTEETE------ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAP 319

Query: 320 VRSSKRYVIRIKAGVY-RENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATM 379
             SS+R+VI +KAG Y  EN++V +KKTN+MFIGDG+  T+ITG +++ D  TTF++AT 
Sbjct: 320 EHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATF 379

Query: 380 AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 439
           AA G GF+ RD+TF+N AGP+KHQAVALRVG D +  Y+C+++ YQD LYVHSNRQF+  
Sbjct: 380 AATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRE 439

Query: 440 CLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 499
           C + GTVDFIFGNAA I Q C+I+ARKP + QK  +TAQ R DPNQNTGI I   ++ AT
Sbjct: 440 CEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLAT 499

Query: 500 SDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNT 559
            DL   + S+PT+LGRPWK YSR V M   + D IDP+GW EW+G FAL++L+YGEY N 
Sbjct: 500 PDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNK 559

Query: 560 GAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
           G G+  G RV W G+ VI S  EA  FT  +FI G SWL STG  F  GL
Sbjct: 560 GLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CsGy7G020340 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 442.2 bits (1136), Expect = 6.6e-124
Identity = 268/582 (46.05%), Postives = 350/582 (60.14%), Query Frame = 0

Query: 17  VSNSSD-LQKTQKSSKKKLLILTLCSTLLIVGAVIGIVAGTTKSSSKYSGESHAIVKSTC 76
           +SNS+  L    KS K K L L L S + I+G+V    A     ++  + +S       C
Sbjct: 1   MSNSNQPLLSKPKSLKHKNLCLVL-SFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQIC 60

Query: 77  SSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKLCGLKNISHR 136
                 D C + +S      L   N+ D++ + +  +V  +E     V +   +++   R
Sbjct: 61  HGAHDQDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSE-ARIRSNGVR 120

Query: 137 ERIALHDCLETIDETLDELHKAIVDL--NEYPNKKSLNQHADDLKTLLSSAITNQETCLD 196
           ++    DC E +D + D +  ++ +L    Y  +   N H     T LSS +TN  TCL+
Sbjct: 121 DKAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVH-----TWLSSVLTNYMTCLE 180

Query: 197 GFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEE 256
             S  D   N +  +K    ++E + S A   +        A  + KM I  NR      
Sbjct: 181 SIS--DVSVNSKQIVKP---QLEDLVSRARVALAIFVSVLPARDDLKM-IISNR------ 240

Query: 257 DDNNNDDGIQWPEWLSAGDRRLLQSS----SVTPNVVVAADGSGNFRTVAAAVAAAPVRS 316
                     +P WL+A DR+LL+SS     VT NVVVA DG+G F+TV  AVAAAP  S
Sbjct: 241 ----------FPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENS 300

Query: 317 SKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMAAVG 376
           + RYVI +K GVY+E +++ KKK N+M +GDG+  TIITGS NV+DGSTTF SAT+AA G
Sbjct: 301 NTRYVIYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANG 360

Query: 377 EGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVS 436
           +GF+A+DI FQNTAGP+KHQAVALRV AD +   +C + AYQDTLY H+ RQFY +  ++
Sbjct: 361 DGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYIT 420

Query: 437 GTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 496
           GTVDFIFGN+A +FQ+CDI AR P +GQKNM+TAQGR D NQNT I IQK +I A+SDL 
Sbjct: 421 GTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLA 480

Query: 497 PVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTGAGA 556
           PV+ S  TFLGRPWK YSRTVIMQ  I + IDP GW  W G FAL+TL+YGEY NTG GA
Sbjct: 481 PVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGA 540

Query: 557 STGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPF 592
            T  RV WKGF+VI+ + EAE FT  K I GG WL  TG  F
Sbjct: 541 DTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553

BLAST of CsGy7G020340 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 436.0 bits (1120), Expect = 4.8e-122
Identity = 257/536 (47.95%), Postives = 338/536 (63.06%), Query Frame = 0

Query: 60  SSKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEEN 119
           +S  SG +   VK+ CS T  P  C   ++ +      +K++ + ++IS+ + +      
Sbjct: 19  ASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNE-PIKSESEFLKISMKLVLDRAILA 78

Query: 120 YFRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKT 179
                 L G K    RE+ A  DC++  D T+ ++++ +      PN K       D +T
Sbjct: 79  KTHAFTL-GPKCRDTREKAAWEDCIKLYDLTVSKINETM-----DPNVKCSKL---DAQT 138

Query: 180 LLSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEA 239
            LS+A+TN +TC  GF           +L    + +  M +N    + N+    +A  + 
Sbjct: 139 WLSTALTNLDTCRAGFL----------ELGVTDIVLPLMSNN----VSNLLCNTLAINKV 198

Query: 240 KMGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVA 299
               T   K           DG  +P W+  GDR+LLQSS+   N VVA DGSGNF+T+ 
Sbjct: 199 PFNYTPPEK-----------DG--FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIK 258

Query: 300 AAVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTT 359
            A+ AA    S R+VI +K GVY EN+E+ KK  N+M  GDG   TIITGS++V  G+TT
Sbjct: 259 EAIDAA--SGSGRFVIYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTT 318

Query: 360 FNSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSN 419
           FNSAT+AAVG+GF+AR ITF+NTAG S  QAVALR G+DLS FYQC   AYQDTLYVHSN
Sbjct: 319 FNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSN 378

Query: 420 RQFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQK 479
           RQFY +C V GTVDFIFGNAAA+ Q+C+I AR+P S + N +TAQGRSDPNQNTGI+I  
Sbjct: 379 RQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHN 438

Query: 480 SRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFY 539
           SR+ A SDLRPV  S  T+LGRPW++YSRTV M+ ++  +IDP+GW EW G+FAL TLFY
Sbjct: 439 SRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFY 498

Query: 540 GEYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 596
            E+QNTG GAST GRVTW GFRV+ SA+EA  FT G F+ GGSW+ S+  PF+ GL
Sbjct: 499 AEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P839482.9e-24175.44Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048863.2e-24074.05Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Q431437.4e-22970.83Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490065.3e-22769.35Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425341.8e-21968.11Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_004141734.10.099.83pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cu... [more]
KAA0059355.10.095.13pectinesterase-like [Cucumis melo var. makuwa] >TYK03971.1 pectinesterase-like [... [more]
XP_016902864.10.096.30PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038897967.10.084.37pectinesterase-like [Benincasa hispida][more]
XP_023549002.10.084.75pectinesterase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KAM60.099.83Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G447990 PE=3 SV=1[more]
A0A5A7V0J00.095.13Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00168... [more]
A0A1S4E3R00.096.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500802 PE=3 SV=1[more]
A0A6J1GQR00.084.55Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456237 PE=3 SV=1[more]
A0A6J1JSY60.084.21Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488604 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.13.8e-22869.35pectin methylesterase 3 [more]
AT1G53830.11.3e-22068.11pectin methylesterase 2 [more]
AT5G53370.14.2e-12643.05pectin methylesterase PCR fragment F [more]
AT1G11580.16.6e-12446.05methylesterase PCR A [more]
AT2G45220.14.8e-12247.95Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 66..225
e-value: 1.5E-35
score: 134.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 70..225
e-value: 1.2E-28
score: 100.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 49..229
e-value: 4.8E-27
score: 92.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 284..580
e-value: 6.0E-148
score: 491.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 278..595
e-value: 1.2E-127
score: 427.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 67..230
e-value: 1.0E-32
score: 115.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 70..230
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 31..594
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 31..594
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 73..230
e-value: 4.73897E-40
score: 141.422
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 309..328
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 428..437
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 281..594

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G020340.1CsGy7G020340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity