CsGy7G011050 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G011050
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionsubtilisin-like protease SBT3.4
LocationGy14Chr7: 13975633 .. 13977604 (-)
RNA-Seq ExpressionCsGy7G011050
SyntenyCsGy7G011050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTGACAAAATAGAACAAAGTTCCATATTCTATCAAAATGATAAACACTGATGGACATCAATATGCTTCTATTAATCATATTGATATGGACAATGATAGAAGTCGATCAGTGTCTATCATTGATAAAATTCAAAATTTTGGTATATGTTGTAAATATTTTAGTTTATTTTTCTATTTTGAAGAATTTTCCTACTTGTTTTTTTTAGAGTTATGAAAATTGGTTTTTGTTGGAGTGACATTTCTTGCCATACTGGGAAGAAGATGACAAAGTTTGGGTGGGTGACTTTTTTCTTCCTTTTCTTTCTATTGTTGCAACTGTATAGTTAGCAGTGGAAATAAACCATACATTGAAAAATATGAAACACCCTTATGCTTTGTTATTTATTTATTTTGTAGAAGTCATTTTAAATCTAATTTCTAATTTTAGATAGCTGACAAAACTAACCCTAAATTGCGCAAACAAACAAGTCATGAAATTACTAAAAATTACTTAAGGAAACAAGAATCCAAAAAATCCTCCTCTAAGAAACCAACTAATGGAAAACCATTCTATATAATTTACCTTCTTGTTTCTTTCTAAAAAAATGAAGAGAAATACTTCTTTAATTTTCGTCCAAATACTCCCTCTTTCTATATCATTGAAGTTGATAGAATGACAAATACAAAGATGAACTTTTCTTCATTTACCTTGTCTCTTGTTTTATTGTTCACAATCTCTTTGAATTCACATCTTATCTCTGGGATGATATCTGACAAACCTGTGTCCAAGTTTGTTGTACCATCATCAATATATATCGTTTACACGGCTAAAAGGCCTAGCAATGAGAAACCCGAGACATTTTATATTCAAATTCTAGCTTCTGTTATCGACAGGTACACTTTATATGAGAAATTATTCAAAAAGACCATCACAATAACAAATAAATATGTCTACACGAAAAATATTCAATAATGTCACCATAACATCCATTTTGTACATTGTTAGACGTAGATTTTTAAATTTGCATGCTCCTATTCAACGGTGAGATATGAGTTCGATATACTTCTTTTCAAATAAGCTGAACAATTGATACGACTTGATAATTGAATAACTTTTCTTTTTCTAAATACACTCCTTGTTTGAAATGATCATGGATAATTTAGGGTGTGAATTTTTGTTTAGTTTGGTTGCTTTGTTGCAGTAATGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTCAAGAGTTCTATAAATGGCTTTGCTGCTAACTTGACTCCTAATCAAGTGAAGAAGATTTTGGGTACCTATCACTCTTAACATTCAAATCTGACCCTTTTTATTTTTTTAACAAATTAGAATATATGTGATTAAATATTGCTTTGGGACTTTGTCAGCACAACCTGGAGTTCTACATGTTGCAAGGAGTGTTAATTATAATCTGCAAACAGAAGGCAAAAAGAATAATAATGTATAGAAGAGCTTCACATACTCTCTCTCCAAATATGGGTTTGTTGGTTAAATATGCAGGTTAAAACTGCAGATCTTATTATTAATATGATTCGGAGAAAGTTGGAGTTGTTTAGTACTTGTAGTTTCACAAAACCCTCACAAGTAGTCGTTGGAGACTATTCATGAGGATTTATCAAATTATAGTGACTATCCTTGCCAACTAACCTTCGACCAGTGTCTCCACCAACTATTATTGGCCAACACATATCCGACAAGCATCACGTGTAAACCTCTTACCACCCTTTTTCACATGATTTTCAACAACCAATTTTTTTCTACCGGTTTTCCTAGAACCACTTTTGGCCAATCTCCAATGATTTCTTTCTGACAATCGCCCTTGGCCAAACTTTGGGTGACCGCTTTCTAACAATCGTTGCAAAACAACTATCGACCACTGTTGACTATTGTCTAACATTGGTAACAATTTTTTGCTAATGTTTTTCGTCAACCACTCACAGTCAAATTTTATCTATTATTTTTT

mRNA sequence

AAATTTGACAAAATAGAACAAAGTTCCATATTCTATCAAAATGATAAACACTGATGGACATCAATATGCTTCTATTAATCATATTGATATGGACAATGATAGAAGTCGATCAGTGTCTATCATTGATAAAATTCAAAATTTTGGTATATGTTGTAAATATTTTAGTTTATTTTTCTATTTTGAAGAATTTTCCTACTTGTTTTTTTTAGAGTTATGAAAATTGGTTTTTGTTGGAGTGACATTTCTTGCCATACTGGGAAGAAGATGACAAAGTTTGGGTGGGTGACTTTTTTCTTCCTTTTCTTTCTATTGTTGCAACTGTATAGTTAGCAGTGGAAATAAACCATACATTGAAAAATATGAAACACCCTTATGCTTTGTTATTTATTTATTTTGTAGAAGTCATTTTAAATCTAATTTCTAATTTTAGATAGCTGACAAAACTAACCCTAAATTGCGCAAACAAACAAGTCATGAAATTACTAAAAATTACTTAAGGAAACAAGAATCCAAAAAATCCTCCTCTAAGAAACCAACTAATGGAAAACCATTCTATATAATTTACCTTCTTGTTTCTTTCTAAAAAAATGAAGAGAAATACTTCTTTAATTTTCGTCCAAATACTCCCTCTTTCTATATCATTGAAGTTGATAGAATGACAAATACAAAGATGAACTTTTCTTCATTTACCTTGTCTCTTGTTTTATTGTTCACAATCTCTTTGAATTCACATCTTATCTCTGGGATGATATCTGACAAACCTGTGTCCAAGTTTGTTGTACCATCATCAATATATATCGTTTACACGGCTAAAAGGCCTAGCAATGAGAAACCCGAGACATTTTATATTCAAATTCTAGCTTCTGTTATCGACAGGTACACTTTATATGAGAAATTATTCAAAAAGACCATCACAATAACAAATAAATATGTCTACACGAAAAATATTCAATAATGTCACCATAACATCCATTTTGTACATTGTTAGACGTAGATTTTTAAATTTGCATGCTCCTATTCAACGGTGAGATATGAGTTCGATATACTTCTTTTCAAATAAGCTGAACAATTGATACGACTTGATAATTGAATAACTTTTCTTTTTCTAAATACACTCCTTGTTTGAAATGATCATGGATAATTTAGGGTGTGAATTTTTGTTTAGTTTGGTTGCTTTGTTGCAGTAATGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTCAAGAGTTCTATAAATGGCTTTGCTGCTAACTTGACTCCTAATCAAGTGAAGAAGATTTTGGCACAACCTGGAGTTCTACATGTTGCAAGGAGTGTTAATTATAATCTGCAAACAGAAGGCAAAAAGAATAATAATGTATAGAAGAGCTTCACATACTCTCTCTCCAAATATGGGTTTGTTGGTTAAATATGCAGGTTAAAACTGCAGATCTTATTATTAATATGATTCGGAGAAAGTTGGAGTTGTTTAGTACTTGTAGTTTCACAAAACCCTCACAAGTAGTCGTTGGAGACTATTCATGAGGATTTATCAAATTATAGTGACTATCCTTGCCAACTAACCTTCGACCAGTGTCTCCACCAACTATTATTGGCCAACACATATCCGACAAGCATCACGTGTAAACCTCTTACCACCCTTTTTCACATGATTTTCAACAACCAATTTTTTTCTACCGGTTTTCCTAGAACCACTTTTGGCCAATCTCCAATGATTTCTTTCTGACAATCGCCCTTGGCCAAACTTTGGGTGACCGCTTTCTAACAATCGTTGCAAAACAACTATCGACCACTGTTGACTATTGTCTAACATTGGTAACAATTTTTTGCTAATGTTTTTCGTCAACCACTCACAGTCAAATTTTATCTATTATTTTTT

Coding sequence (CDS)

ATGACAAATACAAAGATGAACTTTTCTTCATTTACCTTGTCTCTTGTTTTATTGTTCACAATCTCTTTGAATTCACATCTTATCTCTGGGATGATATCTGACAAACCTGTGTCCAAGTTTGTTGTACCATCATCAATATATATCGTTTACACGGCTAAAAGGCCTAGCAATGAGAAACCCGAGACATTTTATATTCAAATTCTAGCTTCTGTTATCGACAGGTACACTTTATATGAGAAATTATTCAAAAAGACCATCACAATAACAAATAAATATGTCTACACGAAAAATATTCAATAA

Protein sequence

MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKPETFYIQILASVIDRYTLYEKLFKKTITITNKYVYTKNIQ*
Homology
BLAST of CsGy7G011050 vs. NCBI nr
Match: XP_008461963.1 (PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa] >TYK08050.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa])

HSP 1 Score: 106 bits (264), Expect = 5.59e-27
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 0

Query: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKP 60
          MTNTKMNFSS  L LVLLFT+SLNS L  GMIS+K +SKF VPSS++IVYTAKRPSNEKP
Sbjct: 1  MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61 ETFYIQILASVI 72
          E FYIQILASV+
Sbjct: 61 EAFYIQILASVL 72

BLAST of CsGy7G011050 vs. NCBI nr
Match: XP_038896488.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 102 bits (253), Expect = 3.44e-25
Identity = 56/72 (77.78%), Postives = 61/72 (84.72%), Query Frame = 0

Query: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKP 60
          M NTKMNFSSFTL LVLLFTISLNS LIS +ISDK  S+  V SS+YIVYT +RPSNEKP
Sbjct: 1  MANTKMNFSSFTLYLVLLFTISLNSLLISAIISDKSASEIDVLSSLYIVYTTERPSNEKP 60

Query: 61 ETFYIQILASVI 72
          E FYIQILASV+
Sbjct: 61 EAFYIQILASVL 72

BLAST of CsGy7G011050 vs. NCBI nr
Match: XP_038896487.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 96.7 bits (239), Expect = 3.01e-23
Identity = 52/67 (77.61%), Postives = 58/67 (86.57%), Query Frame = 0

Query: 6  MNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKPETFYI 65
          MNFSSFTL LVLLFTISLNS LIS +ISDK  SK  VPSS++IVYT +RPSNE+PE FYI
Sbjct: 1  MNFSSFTLYLVLLFTISLNSLLISAIISDKSASKIDVPSSLHIVYTTERPSNEEPEAFYI 60

Query: 66 QILASVI 72
          QILASV+
Sbjct: 61 QILASVL 67

BLAST of CsGy7G011050 vs. NCBI nr
Match: ADN34190.1 (peptidase [Cucumis melo subsp. melo])

HSP 1 Score: 80.5 bits (197), Expect = 7.74e-17
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0

Query: 20 TISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKPETFYIQILASVI 72
          ++SLNS L  GMIS+K +SKF VPSS++IVYTAKRPSNEKPE FYIQILASV+
Sbjct: 20 SVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVL 72

BLAST of CsGy7G011050 vs. NCBI nr
Match: KAG7025065.1 (Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 52.4 bits (124), Expect = 6.99e-06
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 0

Query: 9  SSFTLSLVLLFTISLNSHLISGMISDKP-----VSKFVVPSSIYIVYTAKRPSNEKPETF 68
          SSF   LVLLF+ISLN  L S ++SD+      VS F  P+ ++IVYT +RP NE+PE +
Sbjct: 8  SSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPA-VHIVYT-ERPHNEEPEAY 67

Query: 69 YIQILASVI 72
          +I+ LASV+
Sbjct: 68 HIRTLASVL 74

BLAST of CsGy7G011050 vs. ExPASy TrEMBL
Match: A0A0A0K630 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G274680 PE=4 SV=1)

HSP 1 Score: 137 bits (344), Expect = 1.88e-39
Identity = 72/73 (98.63%), Postives = 73/73 (100.00%), Query Frame = 0

Query: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKP 60
          MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIY+VYTAKRPSNEKP
Sbjct: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYMVYTAKRPSNEKP 60

Query: 61 ETFYIQILASVID 73
          ETFYIQILASVID
Sbjct: 61 ETFYIQILASVID 73

BLAST of CsGy7G011050 vs. ExPASy TrEMBL
Match: A0A5D3C9P1 (Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001980 PE=4 SV=1)

HSP 1 Score: 106 bits (264), Expect = 2.71e-27
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 0

Query: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKP 60
          MTNTKMNFSS  L LVLLFT+SLNS L  GMIS+K +SKF VPSS++IVYTAKRPSNEKP
Sbjct: 1  MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61 ETFYIQILASVI 72
          E FYIQILASV+
Sbjct: 61 EAFYIQILASVL 72

BLAST of CsGy7G011050 vs. ExPASy TrEMBL
Match: A0A1S3CFT4 (subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=1)

HSP 1 Score: 106 bits (264), Expect = 2.71e-27
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 0

Query: 1  MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKP 60
          MTNTKMNFSS  L LVLLFT+SLNS L  GMIS+K +SKF VPSS++IVYTAKRPSNEKP
Sbjct: 1  MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61 ETFYIQILASVI 72
          E FYIQILASV+
Sbjct: 61 EAFYIQILASVL 72

BLAST of CsGy7G011050 vs. ExPASy TrEMBL
Match: E5GCJ1 (Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.75e-17
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0

Query: 20 TISLNSHLISGMISDKPVSKFVVPSSIYIVYTAKRPSNEKPETFYIQILASVI 72
          ++SLNS L  GMIS+K +SKF VPSS++IVYTAKRPSNEKPE FYIQILASV+
Sbjct: 20 SVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVL 72

BLAST of CsGy7G011050 vs. ExPASy TrEMBL
Match: A0A6J1F6S6 (subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442643 PE=4 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.70e-06
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 0

Query: 9  SSFTLSLVLLFTISLNSHLISGMISDKP-----VSKFVVPSSIYIVYTAKRPSNEKPETF 68
          SSF   LVLLF+ISLN  L S ++SD+      VS F  P+ ++IVYT +RP NE+PE +
Sbjct: 13 SSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPA-VHIVYT-ERPHNEEPEAY 72

Query: 69 YIQILASVI 72
          +I+ LASV+
Sbjct: 73 HIRTLASVL 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_008461963.15.59e-2779.17PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilis... [more]
XP_038896488.13.44e-2577.78subtilisin-like protease SBT3.13 [Benincasa hispida][more]
XP_038896487.13.01e-2377.61subtilisin-like protease SBT3.13 [Benincasa hispida][more]
ADN34190.17.74e-1775.47peptidase [Cucumis melo subsp. melo][more]
KAG7025065.16.99e-0650.72Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
A0A0A0K6301.88e-3998.63Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G27468... [more]
A0A5D3C9P12.71e-2779.17Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3CFT42.71e-2779.17subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=... [more]
E5GCJ13.75e-1775.47Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A6J1F6S63.70e-0650.72subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G011050.2CsGy7G011050.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008233 peptidase activity