CsGy6G022920 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G022920
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr6: 23264417 .. 23265389 (-)
RNA-Seq ExpressionCsGy6G022920
SyntenyCsGy6G022920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTATTATATATTGTGTTATCTAGGTTATATATTTGTTGAAAAATGATATGAACAGAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGGTATAATTTAATTATCTTATTAACGTAACGTTGTTGTTGTTTATTATTATTCTTATATAAATATTTAAATGAATATTCTAAATACTTAATTACAGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGTAAGTAGTAACTCTCAAATTCTTTTTACCTTTTTTCAGACTAAGATTACATTATGCTTAGTAATGACATGAAGCATGCTAGTTTTACTATGTTTGTTATTCTTAAAAGTTATACTATTATGATCAAAATTATAATTAAAAGTTAGATTCAAAATGCATGGGGTCAAATTGAATGCTTTTTTTTCTTGTATTTTTGCAGGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

mRNA sequence

ATGGTTAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Coding sequence (CDS)

ATGGTTAACTCATCACCAAGGCCAGATGGGGTAAGAAAAGGAGCACAAGCATTAGCGGCAAGATTTATGGGAACTAAAGCCGCAATTTACAATTGCAAATTCCTTGGATTTCAAGATACCCTTTGTGACGATGACGGTTTACATTTCTACAAAGATTGCTTCATTCAAGGCACTGTTGATTTCATTTTCGGAAAAGGAACTTCCCTTTATTTGAACACCCAATTGGACGTGGCTGGAGATGGAGGATTGGCAGTGATAACAGCCCATTCAAGAGAACAAGAAGCTGACACCAGTGGTTATTCGTTTGTTCATTGTAGCATTACTGGAACTGGCGGTAAAAACACTTACTTGGGAAGAGCTTGGATGCCAAGATCTAGAGTTGTCTTTGCCTACACTACAATAGCTGATATCATTCATCCCGAAGGCTGGAATGACATGAAGCATGCAGGTTTCGACAAGACGGTTATGTTCGGAGAATATAAGTGTTCAGGGCCAGGAGCTGTGTCGACCGGACGTGTAGCCTACGGCAAGCAATTGACTGATGTAGAAGTGAAGCCATTTCTTGGTCTGGAATACGTGCAAAGTGAGAAGTGGTTGCTTCCTCCTCCCAGTCCTCAAGTTTAG

Protein sequence

MVNSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPSPQV*
Homology
BLAST of CsGy6G022920 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 1.6e-69
Identity = 118/202 (58.42%), Postives = 149/202 (73.76%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NS+P PDG RKGAQAL+ R  G KAA YNCKF G+QDT+CDD G HF+KDC+I+GT DFI
Sbjct: 138 NSAPMPDGKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFI 197

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG G SLYL TQL+V GD G+ VITAH+ +  A+ SGYSFVHC +TGT G   YLGR+WM
Sbjct: 198 FGSGRSLYLGTQLNVVGD-GIRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWM 257

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT ++ +++P GW + + AG DKTV +GEYKC+G G+    RV Y + + D+
Sbjct: 258 SHPKVVYAYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDI 317

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E K F+ L Y+Q   WLLPPPS
Sbjct: 318 EAKYFISLGYIQGSSWLLPPPS 337

BLAST of CsGy6G022920 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 4.3e-67
Identity = 115/202 (56.93%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFI
Sbjct: 161 NTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFI 220

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM
Sbjct: 221 FGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWM 280

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D 
Sbjct: 281 SHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDK 340

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E   FL L Y+Q  KWLLPPP+
Sbjct: 341 EANRFLSLGYIQGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 255.4 bits (651), Expect = 5.7e-67
Identity = 115/202 (56.93%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFI
Sbjct: 161 NTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFI 220

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM
Sbjct: 221 FGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWM 280

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D 
Sbjct: 281 SHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDK 340

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E   FL L Y+Q  KWLLPPP+
Sbjct: 341 EANCFLSLGYIQGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.1e-61
Identity = 107/204 (52.45%), Postives = 142/204 (69.61%), Query Frame = 0

Query: 1   MVNSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVD 60
           ++N+SP P    +G QALA R  G KAA YNC+F GFQDTLCDD G HF+K+C+I+GT D
Sbjct: 160 IINTSPMPKPGTQG-QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYD 219

Query: 61  FIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRA 120
           FIFG+G SLYL TQL   GD GL VI AH+R+   + +GYSFVHC +TG  G   YLGRA
Sbjct: 220 FIFGRGASLYLTTQLHAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRA 279

Query: 121 WMPRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT 180
           WM   +VV++YT ++ +++P GW + +    DKTV +GEY C+GPG+    RVA+ + + 
Sbjct: 280 WMSHPKVVYSYTEMSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDID 339

Query: 181 DVEVKPFLGLEYVQSEKWLLPPPS 205
           + E   FL L Y++  KWLLPPP+
Sbjct: 340 NKEASQFLTLGYIKGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-60
Identity = 108/201 (53.73%), Postives = 139/201 (69.15%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P P    +G QALA R    KAA Y+C+F GFQDTLCDD G HF+KDC+I+GT DFI
Sbjct: 162 NTAPMPKPGSQG-QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFI 221

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG+G SLYLNTQL   GD GL VITA  R+   + +GY+FVHC +TGT G   YLGR+WM
Sbjct: 222 FGRGASLYLNTQLHAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWM 281

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+A+T +  +++P GW +  + G+DKTV +GEYKC GPG+    RV Y + +   
Sbjct: 282 SHPKVVYAFTEMTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKN 341

Query: 183 EVKPFLGLEYVQSEKWLLPPP 204
           EV PFL L Y++   WLLPPP
Sbjct: 342 EVTPFLTLGYIKGSTWLLPPP 359

BLAST of CsGy6G022920 vs. NCBI nr
Match: XP_011657599.1 (putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 429 bits (1102), Expect = 3.98e-149
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 166 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 225

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM
Sbjct: 226 FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 285

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV
Sbjct: 286 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 345

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EVKPFLGLEYVQSEKWLLPPPSPQV
Sbjct: 346 EVKPFLGLEYVQSEKWLLPPPSPQV 370

BLAST of CsGy6G022920 vs. NCBI nr
Match: XP_016900732.1 (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 402 bits (1033), Expect = 1.01e-138
Identity = 190/205 (92.68%), Postives = 197/205 (96.10%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 160 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFI 219

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLY+NTQLDVAGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWM
Sbjct: 220 FGKGTSLYVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWM 279

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTT+ADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D 
Sbjct: 280 PRSRVVFAYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDA 339

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EV PFL L YVQSEKWLLPPP+PQV
Sbjct: 340 EVNPFLSLAYVQSEKWLLPPPNPQV 364

BLAST of CsGy6G022920 vs. NCBI nr
Match: KAA0061749.1 (pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase PPME1-like [Cucumis melo var. makuwa])

HSP 1 Score: 402 bits (1033), Expect = 1.16e-138
Identity = 190/205 (92.68%), Postives = 197/205 (96.10%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 164 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFI 223

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLY+NTQLDVAGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWM
Sbjct: 224 FGKGTSLYVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWM 283

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTT+ADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D 
Sbjct: 284 PRSRVVFAYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDA 343

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EV PFL L YVQSEKWLLPPP+PQV
Sbjct: 344 EVNPFLSLAYVQSEKWLLPPPNPQV 368

BLAST of CsGy6G022920 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 379 bits (974), Expect = 1.09e-129
Identity = 175/205 (85.37%), Postives = 192/205 (93.66%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDG+RKGAQALAAR MGTKAAIYNCKF+GFQDTLCDD+GLH YKDCFIQGTVDFI
Sbjct: 164 NSSPRPDGIRKGAQALAARLMGTKAAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFI 223

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLYLN++LDV GDGGLAVITAHSRE+E+D+SGYSF HCSITGTGGKNTYLGRAWM
Sbjct: 224 FGKGTSLYLNSRLDVVGDGGLAVITAHSREEESDSSGYSFAHCSITGTGGKNTYLGRAWM 283

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTT+ADIIH EGWNDM H+G+D TV+FGEYKCSGPGA  +GRV Y KQL+DV
Sbjct: 284 PRSRVVFAYTTMADIIHAEGWNDMNHSGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDV 343

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EVKPF+ L Y+QSEKWLLPPP+PQV
Sbjct: 344 EVKPFISLGYLQSEKWLLPPPNPQV 368

BLAST of CsGy6G022920 vs. NCBI nr
Match: KGN48350.2 (hypothetical protein Csa_003568 [Cucumis sativus])

HSP 1 Score: 379 bits (972), Expect = 2.19e-129
Identity = 181/205 (88.29%), Postives = 193/205 (94.15%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N+SPRP+G RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+
Sbjct: 166 NTSPRPNG-RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFV 225

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLYLNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+T+LGRAWM
Sbjct: 226 FGKGTSLYLNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTFLGRAWM 285

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRV+FAYT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV
Sbjct: 286 PRSRVIFAYTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 345

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EVKPFLGLEYVQSEKWLLPPPSPQV
Sbjct: 346 EVKPFLGLEYVQSEKWLLPPPSPQV 368

BLAST of CsGy6G022920 vs. ExPASy TrEMBL
Match: A0A0A0KEH2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 429 bits (1102), Expect = 1.92e-149
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 166 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 225

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM
Sbjct: 226 FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 285

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV
Sbjct: 286 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 345

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EVKPFLGLEYVQSEKWLLPPPSPQV
Sbjct: 346 EVKPFLGLEYVQSEKWLLPPPSPQV 370

BLAST of CsGy6G022920 vs. ExPASy TrEMBL
Match: A0A1S4DXM9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1)

HSP 1 Score: 402 bits (1033), Expect = 4.91e-139
Identity = 190/205 (92.68%), Postives = 197/205 (96.10%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 160 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFI 219

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLY+NTQLDVAGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWM
Sbjct: 220 FGKGTSLYVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWM 279

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTT+ADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D 
Sbjct: 280 PRSRVVFAYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDA 339

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EV PFL L YVQSEKWLLPPP+PQV
Sbjct: 340 EVNPFLSLAYVQSEKWLLPPPNPQV 364

BLAST of CsGy6G022920 vs. ExPASy TrEMBL
Match: A0A5A7V7V3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480 PE=4 SV=1)

HSP 1 Score: 402 bits (1033), Expect = 5.63e-139
Identity = 190/205 (92.68%), Postives = 197/205 (96.10%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NSSPRPDGVRKGAQALAARFMGTKAAIYNCKF GFQDTLCDDDGLHFYKDCFIQGTVDFI
Sbjct: 164 NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFI 223

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLY+NTQLDVAGDGGLAVITAHSREQE+D SGYSFVHCSITGTGGKNT+LGRAWM
Sbjct: 224 FGKGTSLYVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWM 283

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRVVFAYTT+ADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPG+VST RV YGKQL+D 
Sbjct: 284 PRSRVVFAYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDA 343

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQV 207
           EV PFL L YVQSEKWLLPPP+PQV
Sbjct: 344 EVNPFLSLAYVQSEKWLLPPPNPQV 368

BLAST of CsGy6G022920 vs. ExPASy TrEMBL
Match: A0A6J1DHP7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1)

HSP 1 Score: 370 bits (950), Expect = 2.34e-126
Identity = 172/206 (83.50%), Postives = 190/206 (92.23%), Query Frame = 0

Query: 1   MVNSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVD 60
           +VNSSPRPDG RKGAQALAAR MGTKAA+YNCKF+GFQDTLCDDDGLH YKDCFIQGTVD
Sbjct: 162 IVNSSPRPDGKRKGAQALAARLMGTKAAVYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVD 221

Query: 61  FIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRA 120
           FIFGKGTSLYLN+QLDV GDGGLAVITAHSRE+E+D SGYSFVH SITGTGGKNTYLGRA
Sbjct: 222 FIFGKGTSLYLNSQLDVVGDGGLAVITAHSREEESDPSGYSFVHGSITGTGGKNTYLGRA 281

Query: 121 WMPRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT 180
           WM RSR VFAYTT+ADII+PEGWNDMKH GFD TV+FGEYKCSGPG+  +GRV Y KQL+
Sbjct: 282 WMARSRTVFAYTTMADIINPEGWNDMKHPGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLS 341

Query: 181 DVEVKPFLGLEYVQSEKWLLPPPSPQ 206
           DVEVKPF+ L ++QS+KWLLPPP+P+
Sbjct: 342 DVEVKPFVSLGFLQSDKWLLPPPNPE 367

BLAST of CsGy6G022920 vs. ExPASy TrEMBL
Match: A0A0A0KJZ4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428040 PE=4 SV=1)

HSP 1 Score: 367 bits (942), Expect = 1.44e-125
Identity = 174/204 (85.29%), Postives = 189/204 (92.65%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N+SPRP+G RK AQALAARF GTK+A YNCKFLGFQDTLCDDDGLH YKDCFIQGTVDF+
Sbjct: 137 NTSPRPNG-RKEAQALAARFRGTKSAFYNCKFLGFQDTLCDDDGLHLYKDCFIQGTVDFV 196

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FGKGTSLYLNT+L+V G+G  AVITAHSREQEAD SGYSFVHCSITG G K+TYLGRAWM
Sbjct: 197 FGKGTSLYLNTELNVVGEGQFAVITAHSREQEADASGYSFVHCSITGNG-KDTYLGRAWM 256

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
           PRSRV+FAYT++ DIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT+ 
Sbjct: 257 PRSRVIFAYTSMIDIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTEA 316

Query: 183 EVKPFLGLEYVQSEKWLLPPPSPQ 206
           EVKP+L LE+VQS KWLLPPP+PQ
Sbjct: 317 EVKPYLSLEFVQSAKWLLPPPNPQ 338

BLAST of CsGy6G022920 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-70
Identity = 118/202 (58.42%), Postives = 149/202 (73.76%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           NS+P PDG RKGAQAL+ R  G KAA YNCKF G+QDT+CDD G HF+KDC+I+GT DFI
Sbjct: 138 NSAPMPDGKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFI 197

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG G SLYL TQL+V GD G+ VITAH+ +  A+ SGYSFVHC +TGT G   YLGR+WM
Sbjct: 198 FGSGRSLYLGTQLNVVGD-GIRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWM 257

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT ++ +++P GW + + AG DKTV +GEYKC+G G+    RV Y + + D+
Sbjct: 258 SHPKVVYAYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDI 317

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E K F+ L Y+Q   WLLPPPS
Sbjct: 318 EAKYFISLGYIQGSSWLLPPPS 337

BLAST of CsGy6G022920 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 3.1e-68
Identity = 115/202 (56.93%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFI
Sbjct: 161 NTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFI 220

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM
Sbjct: 221 FGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWM 280

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D 
Sbjct: 281 SHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDK 340

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E   FL L Y+Q  KWLLPPP+
Sbjct: 341 EANRFLSLGYIQGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 4.0e-68
Identity = 115/202 (56.93%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P PDG  KGAQAL+ R  G  AA YNCKF GFQDT+CDD G HF+KDC+++GT DFI
Sbjct: 161 NTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFI 220

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG GTS+YL TQL V GD G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM
Sbjct: 221 FGSGTSMYLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWM 280

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+AYT +  +++P GW + K    DKTV +GEYKCSGPG+    RV + + + D 
Sbjct: 281 SHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDK 340

Query: 183 EVKPFLGLEYVQSEKWLLPPPS 205
           E   FL L Y+Q  KWLLPPP+
Sbjct: 341 EANCFLSLGYIQGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 4.3e-62
Identity = 107/204 (52.45%), Postives = 142/204 (69.61%), Query Frame = 0

Query: 1   MVNSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVD 60
           ++N+SP P    +G QALA R  G KAA YNC+F GFQDTLCDD G HF+K+C+I+GT D
Sbjct: 160 IINTSPMPKPGTQG-QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYD 219

Query: 61  FIFGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRA 120
           FIFG+G SLYL TQL   GD GL VI AH+R+   + +GYSFVHC +TG  G   YLGRA
Sbjct: 220 FIFGRGASLYLTTQLHAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRA 279

Query: 121 WMPRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLT 180
           WM   +VV++YT ++ +++P GW + +    DKTV +GEY C+GPG+    RVA+ + + 
Sbjct: 280 WMSHPKVVYSYTEMSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDID 339

Query: 181 DVEVKPFLGLEYVQSEKWLLPPPS 205
           + E   FL L Y++  KWLLPPP+
Sbjct: 340 NKEASQFLTLGYIKGSKWLLPPPA 360

BLAST of CsGy6G022920 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-61
Identity = 108/201 (53.73%), Postives = 139/201 (69.15%), Query Frame = 0

Query: 3   NSSPRPDGVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFI 62
           N++P P    +G QALA R    KAA Y+C+F GFQDTLCDD G HF+KDC+I+GT DFI
Sbjct: 162 NTAPMPKPGSQG-QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFI 221

Query: 63  FGKGTSLYLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWM 122
           FG+G SLYLNTQL   GD GL VITA  R+   + +GY+FVHC +TGT G   YLGR+WM
Sbjct: 222 FGRGASLYLNTQLHAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWM 281

Query: 123 PRSRVVFAYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDV 182
              +VV+A+T +  +++P GW +  + G+DKTV +GEYKC GPG+    RV Y + +   
Sbjct: 282 SHPKVVYAFTEMTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKN 341

Query: 183 EVKPFLGLEYVQSEKWLLPPP 204
           EV PFL L Y++   WLLPPP
Sbjct: 342 EVTPFLTLGYIKGSTWLLPPP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKF31.6e-6958.42Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
Q84WM74.3e-6756.93Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9LY195.7e-6756.93Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q9LY186.1e-6152.45Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1[more]
Q9LY172.3e-6053.73Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011657599.13.98e-149100.00putative pectinesterase 63 [Cucumis sativus][more]
XP_016900732.11.01e-13892.68PREDICTED: pectinesterase PPME1-like [Cucumis melo][more]
KAA0061749.11.16e-13892.68pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase ... [more]
XP_038902684.11.09e-12985.37putative pectinesterase 63 [Benincasa hispida][more]
KGN48350.22.19e-12988.29hypothetical protein Csa_003568 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0KEH21.92e-149100.00Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1[more]
A0A1S4DXM94.91e-13992.68Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1[more]
A0A5A7V7V35.63e-13992.68Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480... [more]
A0A6J1DHP72.34e-12683.50Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1[more]
A0A0A0KJZ41.44e-12585.29Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428040 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G61680.11.1e-7058.42Pectin lyase-like superfamily protein [more]
AT1G69940.13.1e-6856.93Pectin lyase-like superfamily protein [more]
AT5G07410.14.0e-6856.93Pectin lyase-like superfamily protein [more]
AT5G07420.14.3e-6252.45Pectin lyase-like superfamily protein [more]
AT5G07430.11.6e-6153.73Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 2..202
e-value: 7.7E-63
score: 214.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 14..194
e-value: 6.4E-38
score: 130.2
NoneNo IPR availablePANTHERPTHR31321:SF30PECTINESTERASEcoord: 1..203
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 1..203
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 55..64
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 13..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G022920.2CsGy6G022920.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0004672 protein kinase activity