CsGy5G013380 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G013380
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAMP dependent ligase
LocationGy14Chr5: 17263155 .. 17264828 (+)
RNA-Seq ExpressionCsGy5G013380
SyntenyCsGy5G013380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAG

mRNA sequence

ATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAG

Coding sequence (CDS)

ATGGAAGATCTGAAGCCAAGGCCTGCTAATTCCTTTCCCCTCACTCCAATCGGCTTCATCGAAAGAGCCGCCATCATTTATGGGGATTCTACCTCCATTATCTACAACTCCACCACTTACACCTGGTCCGAAACTCATCGTCGATGCCTTCAACTCGCCTCTTCCATCTCTTCCTTTGGCATTCAAAGAGGCCATGTTGTCTCTGTCTTCGCTCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTTCCCATGGCTGGTGCCATTCTTAATACCATCAATCTCCGCCTTGACACTCGAACCATCTCCATTCTCCTCCGCCATAGCGAATCCAAACTCATCTTCGTCGATCAACCCTCTTGTGCTCTAATCTTTGACGCTTTGGCCTTGTTCCCGCCAGACATGAAACGACCCTTGCTTGTCCTCATTACCGACGACACTGCAGAGCAAGACTCGTCATTGCCATCCGTTGTTTCCATCCGAGACAATTTTGTGGATTCATATGAGAACATGGTTAAGAGTGGAGATTCTAAGTTCCAATGGATACGACCCTTAAGTGAGTGGGATCCGATTGTTTTGAATTATACTTCTGGAACAACATCAGCGCCAAAGGGAGTGGTAAACTGTCACAGGGGAGTTTTCACGGTGGCACTCGATTCAATTTTGGAATGGGGAGTTCCTAAGCAATCGGTTTATTTATGGACCTTACCAATGTTTCATGGCAATGGTTGGAGTTTCCCTTGGGGAATTGCCGCTGTAGGTGGCACAAATGTTTGTATTCGAAAATTTGATTCAAGATTGATTTTTTCTCTAATTCGTCGTCATCACGTGACACACTTGTGTGGAGCTCCAGTTGTACTCAATATGCTAACAAACTCCCCTGACAATCTACCACTTGATCGACCGGTCAAGATTCTCACTGCCGGAGCACCACCACCCGCATCAGTTCTGCTTCGAACTGAATCACTGGGGTTTGTCGTCACCCATGGGTACGGATTGACTGAGAGTGCAGGGACGATCATCTGCTGTTCATGGAAGTCGGAGTGGAACCAACTCCCAGCAATAGAGCAAGCACGACTGAAGGCGAGGCAGGGCGTACGGATGCTAGCAGTGGAGGCGGTGGATGTGTTGGATCCAGAGACTAGAAAAAGCGTGAAAAGAGATGGAGCTTCCATAGGAGAGATCGTTGTGAGAGGTCCATCGCTAATGCTTGGGTATTTGAAAGATACAGAAGCAACAAGTAAAGCAATGAGCGAGGATGGGTGGTTCTTCACCGGAGATGTTGGGGTGATGCACCCGGATGGGTATATAGAGATAAAGGACCGATCAAAGGATGTGATCATTAGTGGGGGAGAGAATTTGAGCAGTGTTGAGGTTGAATCAGTTTTGTACATGAATCCAATGGTGAATGAGGCGGCAGTGGTGGCACGACCAGACGAGTTCTGGGGAGAGACGCCATGTGCGTTTATAAGCTTGAAAGAAGGATTGAGTCGGAAACCAACAGAAGAAGAGATTATTGAGTATTGCAAAGGGAAGTTACCAAAGTTTATGGTGCCAAAGACAGTGATCTTCATGACGGAAATGCCAAAGACCGCCACAGGGAAATTCCAAAAGTTTGTGCTTAGACAAAAGGCCAAGTCACTAGGCCCTCCACAAAAGAGTAGATTGTAG

Protein sequence

MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKFVLRQKAKSLGPPQKSRL*
Homology
BLAST of CsGy5G013380 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 6.1e-233
Identity = 370/550 (67.27%), Postives = 462/550 (84.00%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPA+S PLTP+GF+ERAA +YGD TS++Y++ +YTWS+THRRCL LASSI+S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           I+ GHVVSV APN P MYELHFAVPMAGAILN +NLRLD RTISILL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           S  LI +A+ALFP     P LV + D++   +SS      +   F  SY++++  GD  F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADESESGNSS-----ELGKEFFCSYKDLIDRGDPDF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           +W+ P SEWDP++LNYTSGTTS+PKGVV+CHRG+F + +DS+++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWS+PWG+AAVGGTN+C+RKFDS +I+ +I+RH VTH+CGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
              V+I+TAGAPPP++VL RTESLGF V+HGYGLTE+AG ++ C+WK EWN LPA E+AR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LK+RQGV  +    +DV+DP T  +VKRDG+++GE+V+RG S+MLGYLKD E T+K+M+ 
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + EAAVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLK+GL++KPTE+EI+EYC+ KLP++MVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540

Query: 541 VLRQKAKSLG 551
           +LR  A+ +G
Sbjct: 541 ILRDMARGMG 545

BLAST of CsGy5G013380 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.7e-220
Identity = 356/552 (64.49%), Postives = 453/552 (82.07%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISS-F 60
           ME+LKPR ANS PLTP+GF+ERAA +YGD  S++Y+  +YTWS+TH RCL++AS I S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           G+++G VVSV APN PAMYEL+FAVPMAG +LN IN RL+  TIS++LRHSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L+ DA++LFP +   PLLVLI D+   ++ +    V  R++FV  YE++V+ GD K
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLT----VEDREHFVCCYEDLVEKGDDK 180

Query: 181 -FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLP 240
            F+W+RP+SEWDPIVLNYTSGTTS+PKGVV+ HR VF V LDS+++WGVPKQ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240

Query: 241 MFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNL 300
           MFH NGW + WGIAAVGGTN+C+R+FD  +IF+LIRRH VTH+C AP+VLNML+NSP+  
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV ++T GAPPPA+VLLRTESLGFV++HGYG+TE  G ++ C+WK EWN+LPA EQ
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           ARLK+RQGVR  A+  VDV+DP +  SVKRDG ++GEIV++G S+MLGYLK++ AT+K +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
             DGWF+TGD+ VMHPDGY+EIKDRSKDVIISGGEN+SSVEVES LY +P V+EAAVVA 
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480

Query: 481 PDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDE+WGETP AF++LK+G+  +PTE+EI+EYC+ KL  FMVPK V+F  ++PKT+TGK Q
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540

Query: 541 KFVLRQKAKSLG 551
           KF L++  K++G
Sbjct: 541 KFTLKEIVKTMG 548

BLAST of CsGy5G013380 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 6.7e-216
Identity = 359/558 (64.34%), Postives = 454/558 (81.36%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CsGy5G013380 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 7.5e-215
Identity = 357/551 (64.79%), Postives = 449/551 (81.49%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CsGy5G013380 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.8e-203
Identity = 342/551 (62.07%), Postives = 435/551 (78.95%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+     + V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of CsGy5G013380 vs. NCBI nr
Match: XP_004148908.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hypothetical protein Csa_023091 [Cucumis sativus])

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CsGy5G013380 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CsGy5G013380 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 449/557 (80.61%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S V  R +FVDSYE MV+ GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CsGy5G013380 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 450/561 (80.21%), Postives = 500/561 (89.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNTPAMYELHFAVPMAGA+LNTINLRLD+RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSV----VSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S     VS R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           D  FQWIRP+SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 557
            QKFVLR+ AKS+GP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CsGy5G013380 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 926 bits (2394), Expect = 0.0
Identity = 447/561 (79.68%), Postives = 497/561 (88.59%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIY+STTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSV----VSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S     V  R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           D  FQWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           ++ARLKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 557
            QKFVLR+ AKS+GP  KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of CsGy5G013380 vs. ExPASy TrEMBL
Match: A0A0A0KMT3 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLRQKAKSLGPPQKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of CsGy5G013380 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CsGy5G013380 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 527/557 (94.61%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILNTINLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SCALIF+ALALFPP+MKRPLLVLI DD AEQDSSLPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTVALDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWGIAAVGGTNVCIRKFD+RLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+ LGYLKD E T+KAMSE
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLRQKAKSLGP  KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of CsGy5G013380 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 449/557 (80.61%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNT AMYELHFAVPMAGA+LNTINLRLD RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S V  R +FVDSYE MV+ GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
           EFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+F  E+PKT+TGK QKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQKAKSLGPPQKSRL 557
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CsGy5G013380 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 450/561 (80.21%), Postives = 500/561 (89.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCLQLASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
           IQRG VVSV +PNTPAMYELHFAVPMAGA+LNTINLRLD+RTIS++LRH E+ LIFVDQ 
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSV----VSIRDNFVDSYENMVKSG 180
           SC LI DALALFPP+MKRP L+LI D+ A +DSS  S     VS R +FVDSYE MV+ G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           D  FQWIRP+SEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS++EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWGIAAVGGTNVCIRKFD+  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA+VL RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKD EAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKEGL+R+PTE EIIEYC+GKLP+FMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 FQKFVLRQKAKSLGPPQKSRL 557
            QKFVLR+ AKS+GP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CsGy5G013380 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 751.5 bits (1939), Expect = 4.8e-217
Identity = 359/558 (64.34%), Postives = 454/558 (81.36%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV I D   +++    + V+ R  F  +Y++++  GD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA+VLLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           D FWGETPCAF+SLK GL+++PTE E+IEYC+ K+PK+MVPKTV F+ E+PKT+TGK  K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQKAKSLGPPQKSRL 558
           FVLR+ AK +G  + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of CsGy5G013380 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 748.0 bits (1930), Expect = 5.3e-216
Identity = 357/551 (64.79%), Postives = 449/551 (81.49%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLQLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCL++ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV +PNTPAMYEL FAVPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
            S  L  +A+++   D   P+LV+I D   E++  +  V  +   F  +Y+++++ GD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIAD--KEEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTSAPKGVV+CHRG+F +++DS+++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWGIAAVGGTNVC+RKFD+ LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA+VLLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG S+MLGYLKD   T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 EDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARP 480
           ++GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNE AVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQK 540
           DEFWGETPCAF+SLK G S KPTEEE++EYC+ K+PK+MVPKTV FM E+PK++TGK  K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQKAKSLG 551
           FVLR  AK +G
Sbjct: 541 FVLRDIAKKMG 545

BLAST of CsGy5G013380 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 709.1 bits (1829), Expect = 2.7e-204
Identity = 342/551 (62.07%), Postives = 435/551 (78.95%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV + NTP MYEL F+VPM+GAILN IN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 PSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSK 180
               L  +A+ +    +  P+LVLI ++  E+     + V+ R  F   Y +++  G+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDPIV+NYTSGTTS+PKGVV+CHRG+F + LDS+ +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWGIAAVGGTNVC+RK  +  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA+VLLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+   T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVAR 480
            ++GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNEAAVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQ 540
           PDEFWGETPCAF+SLK GL+RKPT++EIIEYCK K+P++M PKTV F+ E+PKT+TGK  
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQKAKSL 550
           K +L++ AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of CsGy5G013380 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 689.9 bits (1779), Expect = 1.7e-198
Identity = 330/555 (59.46%), Postives = 428/555 (77.12%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSL-PSVVSIRDNFVDSYENMVKSG 180
           D  S +++ +A++ F    ++P LVL+ DD  ++D SL PS  S   +F+D+Y+ +++ G
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAAS---DFLDTYQGVMERG 180

Query: 181 DSKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWT 240
           DS+F+WIRP +EW P++LNYTSGTTS+PKGVV  HR +F + + S+L+W  P + VYLWT
Sbjct: 181 DSRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWT 240

Query: 241 LPMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGW + WG AAVG TNVC R+ D+  I+ LI +HHVTH+C AP+VLNMLTN P 
Sbjct: 241 LPMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPS 300

Query: 301 NLPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
             PL  PV+++TAGAPPPA+++ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +
Sbjct: 301 RKPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPL 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSK 420
           E+ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++G S+MLGY KD E T+ 
Sbjct: 361 ERARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAA 420

Query: 421 AMSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVV 480
            M EDGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NP+V EAAVV
Sbjct: 421 CMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEGLSRK--PTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTAT 540
           A+PD+ WGETPCAF+SLK   +     TE EI E+CK +LPK+MVP+ VIF  E+PKT+T
Sbjct: 481 AKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKFQKFVLRQKAKSL 550
           GK QKF+LRQ AKSL
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CsGy5G013380 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.7e-196
Identity = 328/556 (58.99%), Postives = 429/556 (77.16%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYN-STTYTWSETHRRCLQLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCL++AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFV 120
           S GI++G VVSV  PN P++YEL FAVPM+GAILN IN RLD   +S+LLRHSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQPSCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGD 180
           D  S +L+ +A++LF    ++P LVL+ DD  E DSS  S      +F+D+YE +++ G+
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLL-DDDQENDSSSAS------DFLDTYEEIMERGN 180

Query: 181 SKFQWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTL 240
           S+F+WIRP +EW P+VLNYTSGTTS+PKGVV  HR +F + + S+L+W VP + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TN+C R+ D+  I++LI +H+VTH+C AP+VLNML N P +
Sbjct: 241 PMFHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLS 300

Query: 301 LPLDRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++T+GAPPPA+++ R ESLGF V+H YGLTE++G ++ C+WK +W+ L  +E
Sbjct: 301 TPLKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKA 420
           +ARLK+RQGVR L    VDV D +T KSVK DG S+GEIV RG S+MLGY KD + T+  
Sbjct: 361 RARLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAAC 420

Query: 421 MSEDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVA 480
           M EDGWF++GD+GV+H DGY+EIKDRSKDVII GGEN+SS E+E+VLY NP+V EAAVVA
Sbjct: 421 MREDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLK---EGLSRKP-TEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTA 540
           +PD+ WGETPCAF+SLK    G    P TE EI E+CK KLPK+MVP+ VIF  E+PKT+
Sbjct: 481 KPDKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKFQKFVLRQKAKSL 550
           TGK QKF+LRQ AK+L
Sbjct: 541 TGKIQKFLLRQMAKTL 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4IQQ56.1e-23367.272-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1[more]
M4ISH27.7e-22064.49Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1[more]
Q9FFE66.7e-21664.34Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FFE97.5e-21564.79Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S... [more]
Q9LQS13.8e-20362.07Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_004148908.10.0100.00probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hyp... [more]
XP_008463174.10.094.61PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004... [more]
XP_022939278.10.080.61probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.... [more]
XP_022993747.10.080.21probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima][more]
XP_023550909.10.079.68probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KMT30.0100.00AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1[more]
A0A5D3D6230.094.61Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CIL00.094.61probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1FL760.080.61probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JTQ20.080.21probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G16370.14.8e-21764.34acyl activating enzyme 5 [more]
AT5G16340.15.3e-21664.79AMP-dependent synthetase and ligase family protein [more]
AT1G75960.12.7e-20462.07AMP-dependent synthetase and ligase family protein [more]
AT1G21540.11.7e-19859.46AMP-dependent synthetase and ligase family protein [more]
AT1G21530.22.7e-19658.99AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 446..549
e-value: 2.5E-29
score: 103.7
NoneNo IPR availablePANTHERPTHR43859:SF50LONG-CHAIN ACYL-COA SYNTHETASEcoord: 2..551
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 2..551
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 13..543
e-value: 0.0
score: 807.678
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 10..548
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 4..445
e-value: 3.3E-95
score: 321.1
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 26..450
e-value: 1.7E-89
score: 300.3
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 459..536
e-value: 2.4E-22
score: 79.7
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G013380.1CsGy5G013380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity