CsGy5G010310 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy5G010310 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 8.6e-230
Identity = 420/772 (54.40%), Postives = 538/772 (69.69%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           M F    ++ +  +   S+V+  +QTY++H  TT             I+ S+   +SL  
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTST---------KHIVTSL--FNSLQT 77

Query: 61  DN-NDDEEALNAAEILYVYK--------TLSSRNLHSLSKVPGFVAATPNELLQLHTTHS 120
           +N NDD+ +L   EI Y+Y+        TL+   L ++    GF++A P+ELL LHTT+S
Sbjct: 78  ENINDDDFSL--PEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYS 137

Query: 121 PQFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNF 180
            +FLGL+ G GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVPS+W+G C  G NF
Sbjct: 138 HEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNF 197

Query: 181 SHSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFY 240
           S S CNKK+IGA  F + YE+ VG++N T  FRS RD+ GHGTHTASTAAG+ + +A+++
Sbjct: 198 SSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYF 257

Query: 241 NQGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYS 300
            Q  G+A+GMRFTSRIA+YK CW  GCAS D++AA+D A+ DGVDV+S+SLGG S   Y 
Sbjct: 258 GQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYV 317

Query: 301 DQIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGK 360
           D IAIA FGA+QK +FVSCSAGNSGP  STVSN APW+MTVAASYTDRTFP  VR+GN K
Sbjct: 318 DPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRK 377

Query: 361 VFEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 420
              GSS Y GK+LK +PL +N TAG+     FC   SL   +V GKIV+C RG + RT K
Sbjct: 378 SLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAK 437

Query: 421 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASII 480
           GE+VK +GGA M+L++T  EGE+LLAD HVLPA S+G S  K++LNY+A +   A AS+ 
Sbjct: 438 GEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVR 497

Query: 481 FKGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRV 540
           F+GT YG+ AP VAAFSSRGPS   P + KPDI APG+NILA W P  SPS L SD RRV
Sbjct: 498 FRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRV 557

Query: 541 LFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASG 600
            FNIISGTSM+CPH+SG+AAL+KSVH DWSPA IKSA+MTTA +TDN+   I D G A  
Sbjct: 558 QFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGA 617

Query: 601 -GPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSK 660
              A +FAFG+G+VDP +A  PGL+YD +  DY+ YLCSL YTS +I L S   +TC+S 
Sbjct: 618 ESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASN 677

Query: 661 NTFSQPGDLNYPSFSVFMKKGKNVNST-FKRTVTNVGIPRSDYTVRINNPKGIRIIVKPE 720
                PGDLNYPSF+V +  G N+ +  +KRTVTNVG P  +Y V +  PKG+++ V+P+
Sbjct: 678 AVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPK 737

Query: 721 KLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 762
            L F K  E+LSY V++ A   R S    SFG LVW    Y VRSPIAVTW+
Sbjct: 738 VLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of CsGy5G010310 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 3.9e-190
Identity = 363/771 (47.08%), Postives = 490/771 (63.55%), Query Frame = 0

Query: 9   LLYITMLTTSSVAMDQQTYIVHMDTTKMDTP--NPEQWYTAIIDSVNQLSSLYGDNNDDE 68
           L+++   TT+ ++  ++TY++HMD + M  P  N  QWY++ I+SV Q       +   E
Sbjct: 20  LIFLQAETTTQIS-TKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQ-------HKSQE 79

Query: 69  EALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQ 128
           E  N   ILY Y+T        L+      L +  G VA  P    +LHTT SP FLGL+
Sbjct: 80  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 139

Query: 129 R--GHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 188
           R     +W       D+++GVLDTGIWPE  SF D G+ PVP+ W+G C+TG  F   NC
Sbjct: 140 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 199

Query: 189 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 248
           N+K++GAR F + YEAA G+++    ++S RD +GHGTHTA+T AG+ +  A+ +    G
Sbjct: 200 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 259

Query: 249 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 308
            A GM   +R+A+YKVCW  GC S+DIL+A+D AVADGV VLSISLGGG S    D ++I
Sbjct: 260 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 319

Query: 309 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 368
           A FGA++ GVFVSCSAGN GP   +++NV+PW+ TV AS  DR FP TV++G  + F+G 
Sbjct: 320 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 379

Query: 369 SSYFGKNL----KEVPLVY-NNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 428
           S Y G+ +    K+ PLVY    A     T+FC  G+LD   V GKIV+C+RG   R +K
Sbjct: 380 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 439

Query: 429 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASII 488
           G+ VK AGG GM+L NT   GE+L+ADSH+LPA +VG    K I  Y  +SK+ A AS+ 
Sbjct: 440 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK-ATASLE 499

Query: 489 FKGTKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 548
             GT+ G + +P VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++PS L SD RR
Sbjct: 500 ILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRR 559

Query: 549 VLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRAS 608
           V FNI+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAYV DN    ++D   A+
Sbjct: 560 VKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA 619

Query: 609 GGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSK 668
             P+  +  G+GH+DP +A+ PGL+YDI PQ+Y  +LC+   + +Q+ + ++        
Sbjct: 620 --PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKH 679

Query: 669 NTFSQPGDLNYPSFSVFMKKGKNVNS-TFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPE 728
                PG+LNYP+ S    +  +V + T +RTVTNVG   S Y V ++  KG  + V+P+
Sbjct: 680 TLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 729 KLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
            LNF    +KLSY V+F     R  +    FG LVW S T+ VRSP+ +TW
Sbjct: 740 TLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of CsGy5G010310 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.8e-188
Identity = 371/780 (47.56%), Postives = 485/780 (62.18%), Query Frame = 0

Query: 9   LLYITMLTTSSVAMDQQTYIVHMDTTKMDT--PNPEQWYTAIIDSVNQLSSLYGDNNDDE 68
           LL ++  ++S+ + +  TYIVH+D     +  P    WYT+ + S+              
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS------------ 69

Query: 69  EALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQ 128
              +   I++ Y T        L+S++   L   P  ++  P ++  LHTT SP+FLGL+
Sbjct: 70  ---SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLR 129

Query: 129 RGH--GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 188
                GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP KWKG C    +F  S C
Sbjct: 130 STDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESAC 189

Query: 189 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 248
           N+KL+GAR F   YEA  G++N T  FRS RDS+GHGTHTAS +AG ++  AS      G
Sbjct: 190 NRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 249

Query: 249 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 308
           VA GM   +R+A+YKVCW  GC  +DILAA D AVADGVDV+S+S+GG     Y D IAI
Sbjct: 250 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 309

Query: 309 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 368
            AFGAI +G+FVS SAGN GP   TV+NVAPW+ TV A   DR FP  V+LGNGK+  G 
Sbjct: 310 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 369

Query: 369 SSYFGKNL---KEVPLVYNNT--AGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 428
           S Y G  L   +  PLVY  +   GDG  ++ C  GSLDP +V+GKIV+C+RG NSR  K
Sbjct: 370 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 429

Query: 429 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAK---- 488
           GE V+  GG GMI+ N + +GE L+AD HVLPATSVGAS    I  YI+ S +       
Sbjct: 430 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHP 489

Query: 489 -ASIIFKGTKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELE 548
            A+I+FKGT+ G R AP VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + PS + 
Sbjct: 490 TATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVT 549

Query: 549 SDKRRVLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISD 608
           SD RR  FNI+SGTSM+CPHVSGLAAL+K+ H DWSPAAI+SAL+TTAY  DN    + D
Sbjct: 550 SDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD 609

Query: 609 VGRASGGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKF 668
              ++G  +    +GSGHV P KA  PGL+YDI   DYI +LC+  YT T I  ++R + 
Sbjct: 610 --ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 669

Query: 669 TCSSKNTFSQPGDLNYPSFS-VFMKKGKNVNST-FKRTVTNVGIPRSDYTVRINNPKGIR 728
            C         G+LNYPSFS VF + G++  ST F RTVTNVG   S Y ++I  P+G  
Sbjct: 670 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 729

Query: 729 IIVKPEKLNFVKLGEKLSY--KVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 762
           + V+PEKL+F ++G+KLS+  +V    +           G +VW  G   V SP+ VT Q
Sbjct: 730 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of CsGy5G010310 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 3.2e-184
Identity = 365/772 (47.28%), Postives = 486/772 (62.95%), Query Frame = 0

Query: 8   LLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNP--EQWYTAIIDSVNQLSSLYGDNNDD 67
           LLL +     SS + DQ TYIVHM  ++M +       WY + + S++            
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS------------ 72

Query: 68  EEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGL 127
               ++AE+LY Y+         L+     SL   PG ++  P    +LHTT +P FLGL
Sbjct: 73  ----DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 132

Query: 128 -QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 187
            +    L+  +   SD+++GVLDTG+WPE  S+ D+G  P+PS WKG C+ G NF+ S C
Sbjct: 133 DEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 192

Query: 188 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 247
           N+KLIGAR F + YE+ +G ++ +   RS RD +GHGTHT+STAAG+ +  AS      G
Sbjct: 193 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 252

Query: 248 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 307
            A GM   +R+A YKVCW  GC S+DILAA+D A+AD V+VLS+SLGGG S  Y D +AI
Sbjct: 253 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 312

Query: 308 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 367
            AF A+++G+ VSCSAGN+GP  S++SNVAPW+ TV A   DR FP    LGNGK F G 
Sbjct: 313 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 372

Query: 368 SSYFGKNL--KEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQ 427
           S + G+ L  K +P +Y   A +    N C  G+L P  V+GKIV+C+RG N+R +KG+ 
Sbjct: 373 SLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDV 432

Query: 428 VKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKG 487
           VK AGG GMIL NT   GE+L+AD+H+LPAT+VG  A   I +Y+ +      ASI   G
Sbjct: 433 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT-ASISILG 492

Query: 488 TKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLF 547
           T  G + +P VAAFSSRGP+   P ++KPD+ APGVNILAAW     P+ L SD RRV F
Sbjct: 493 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 552

Query: 548 NIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGP 607
           NIISGTSMSCPHVSGLAAL+KSVH +WSPAAI+SALMTTAY T      + D+  A+G P
Sbjct: 553 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKP 612

Query: 608 ADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTF 667
           +  F  G+GHV P  A++PGLIYD+  +DY+ +LC+L YTS QI  VSR  +TC    ++
Sbjct: 613 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 672

Query: 668 SQPGDLNYPSFSVFMKKGKNVNST----FKRTVTNVGIPRSDYTVRI-NNPKGIRIIVKP 727
           S   DLNYPSF+V      NV+      + RTVT+VG     Y+V++ +   G++I V+P
Sbjct: 673 S-VADLNYPSFAV------NVDGVGAYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEP 732

Query: 728 EKLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
             LNF +  EK SY V+F     + S    SFGS+ W  G + V SP+A++W
Sbjct: 733 AVLNFKEANEKKSYTVTFTVDSSKPS-GSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of CsGy5G010310 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.1e-180
Identity = 358/764 (46.86%), Postives = 479/764 (62.70%), Query Frame = 0

Query: 6   VLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNP----EQWYTAIIDSVNQLSSLYGD 65
           +   L++ + TT+     ++TYI+ ++ +  D P        WYT+ ++S + L   Y  
Sbjct: 14  ITTFLFLLLHTTA-----KKTYIIRVNHS--DKPESFLTHHDWYTSQLNSESSLLYTYTT 73

Query: 66  NNDDEEALNAAEILYVYKTLSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGH 125
           +     A       Y+  T +   L S + +   +    + L  LHTT +P+FLGL    
Sbjct: 74  SFHGFSA-------YLDSTEADSLLSSSNSI---LDIFEDPLYTLHTTRTPEFLGLNSEF 133

Query: 126 GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLI 185
           G+ +  + ++ +IIGVLDTG+WPE  SF D  +P +PSKWKG C++G +F    CNKKLI
Sbjct: 134 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 193

Query: 186 GARTFIQAYE-AAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATG 245
           GAR+F + ++ A+ G  +      S RD +GHGTHT++TAAG+ +  ASF     G A G
Sbjct: 194 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 253

Query: 246 MRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFG 305
           M   +R+A+YKVCW  GC  +DILAAMD A+ DGVDVLS+SLGGGS+  Y D IAI AF 
Sbjct: 254 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 313

Query: 306 AIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYF 365
           A+++GVFVSCSAGNSGP  ++V+NVAPWVMTV A   DR FP    LGNGK   G S Y 
Sbjct: 314 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 373

Query: 366 GKNL--KEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLA 425
           G  +  K + LVYN   G+   +N C  GSLD ++VRGKIVVC+RG N+R +KG  V+ A
Sbjct: 374 GVGMGTKPLELVYNK--GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 433

Query: 426 GGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYG 485
           GG GMI+ NT   GE+L+ADSH+LPA +VG      +  Y+ S  +   A ++FKGT   
Sbjct: 434 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPT-ALLVFKGTVLD 493

Query: 486 SR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIIS 545
            + +P VAAFSSRGP+   P ++KPD+  PGVNILA W   + P+ L+ D RR  FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMS 553

Query: 546 GTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSF 605
           GTSMSCPH+SGLA L+K+ H +WSP+AIKSALMTTAYV DN    + D   A    ++ +
Sbjct: 554 GTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSNPY 613

Query: 606 AFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQI-SLVSRGKFTCSSKNTFSQP 665
           A GSGHVDP+KA  PGL+YDI+ ++YI +LCSL YT   I ++V R    CS K  FS P
Sbjct: 614 AHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDP 673

Query: 666 GDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKL 725
           G LNYPSFSV    GK V   + R VTNVG   S Y V +N    + I VKP KL+F  +
Sbjct: 674 GQLNYPSFSVLF-GGKRV-VRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSV 733

Query: 726 GEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
           GEK  Y V+F +       ++  FGS+ W +  + VRSP+A +W
Sbjct: 734 GEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of CsGy5G010310 vs. NCBI nr
Match: XP_004140477.2 (subtilisin-like protease SBT1.1 [Cucumis sativus] >XP_011659836.1 subtilisin-like protease SBT1.1 [Cucumis sativus] >KAE8648138.1 hypothetical protein Csa_021525 [Cucumis sativus])

HSP 1 Score: 1504 bits (3894), Expect = 0.0
Identity = 761/769 (98.96%), Postives = 761/769 (98.96%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG
Sbjct: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60

Query: 61  DNNDDEEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120
           DNNDDEEALNAAEILYVYKT        LSSRNLHSLSKVPGFVAATPNELLQLHTTHSP
Sbjct: 61  DNNDDEEALNAAEILYVYKTVISGFSAKLSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120

Query: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180
           QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS
Sbjct: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180

Query: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240
           HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN
Sbjct: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240

Query: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300
           QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD
Sbjct: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300

Query: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360
           QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV
Sbjct: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360

Query: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420
           FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG
Sbjct: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420

Query: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480
           EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF
Sbjct: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480

Query: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540
           KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL
Sbjct: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540

Query: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600
           FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG
Sbjct: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600

Query: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660
           PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT
Sbjct: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660

Query: 661 FSQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLN 720
           FSQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLN
Sbjct: 661 FSQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLN 720

Query: 721 FVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           FVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ
Sbjct: 721 FVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 769

BLAST of CsGy5G010310 vs. NCBI nr
Match: XP_008454764.1 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo] >XP_008454765.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo])

HSP 1 Score: 1396 bits (3613), Expect = 0.0
Identity = 706/769 (91.81%), Postives = 730/769 (94.93%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           MGFKEVLL LYITMLTTS VAMDQQ+YIVHMDTTKM T NPEQWYTAII SVN+LSSL  
Sbjct: 1   MGFKEVLLFLYITMLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDA 60

Query: 61  DNNDDEEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120
           +NN+ E+ALN AEILYVYKT        LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP
Sbjct: 61  NNNE-EQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTHSP 120

Query: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180
           +FLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDK LPPVPSKWKGICQTGP+FS
Sbjct: 121 KFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFS 180

Query: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240
            SNCNKKLIGARTFIQAYEAAVGRLN TG FRSARDS+GHGTHTASTAAGNF+NRASFYN
Sbjct: 181 LSNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYN 240

Query: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300
           QGMGVATGMRFTSRIA+YKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD
Sbjct: 241 QGMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300

Query: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360
           QIAIAAFGAIQKGVFVSCSAGNSGPFISTV NVAPWVMTVAASYTDRTFPTTVRLGNG V
Sbjct: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMV 360

Query: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420
           FEGSS YFGKNLKE PLVYNNTAGDG+ETNFCT GSLDPTMV+GKI VCERGTNSRT+KG
Sbjct: 361 FEGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKG 420

Query: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480
           EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVG SA KSILNYIASSKRQAKASI+F
Sbjct: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMF 480

Query: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540
           KGTKYGSRAPRVAAFSSRGPSF+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL
Sbjct: 481 KGTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540

Query: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600
           FNIISGTSMSCPHVSGLAAL+KSVHKDWSPAAIKSALMTTAYVTDNK HLISDVGRASGG
Sbjct: 541 FNIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGG 600

Query: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660
           PAD FAFGSGHVDPEKAS PGL+YDIAPQDYITYLCSLKY STQI+LVSRGKFTCSSK T
Sbjct: 601 PADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRT 660

Query: 661 FSQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLN 720
           F QPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+
Sbjct: 661 FFQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLS 720

Query: 721 FVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           FVKLGEKLSYKVSF ALGKR+SLD+FSFGSLVWHSGTY VRSPIAVTWQ
Sbjct: 721 FVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of CsGy5G010310 vs. NCBI nr
Match: KAA0056503.1 (subtilisin-like protease SBT1.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1368 bits (3542), Expect = 0.0
Identity = 690/748 (92.25%), Postives = 714/748 (95.45%), Query Frame = 0

Query: 14  MLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYGDNNDDEEALNAAE 73
           MLTTS VAMDQQ+YIVHMDTTKM T NPEQWYTAII SVN+LSSL  +NN+ E+ALN AE
Sbjct: 1   MLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDANNNE-EQALNIAE 60

Query: 74  ILYVYKTLSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGHGLWNSSNLASDI 133
           ILY    LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP+FLGLQRGHGLWNSSNLASDI
Sbjct: 61  ILY----LSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQRGHGLWNSSNLASDI 120

Query: 134 IIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLIGARTFIQAYEAA 193
           IIGVLDTGIWPEHISFQDK LPPVPSKWKGICQTGP+FS SNCNKKLIGARTFIQAYEAA
Sbjct: 121 IIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKKLIGARTFIQAYEAA 180

Query: 194 VGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATGMRFTSRIASYKVC 253
           VGRLN TG FRSARDS+GHGTHTASTAAGNF+NRASFYNQGMGVATGMRFTSRIA+YKVC
Sbjct: 181 VGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVATGMRFTSRIAAYKVC 240

Query: 254 WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG 313
           WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG
Sbjct: 241 WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG 300

Query: 314 NSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYFGKNLKEVPLVYNN 373
           NSGPFISTV NVAPWVMTVAASYTDRTFPTTVRLGNG VFEGSS YFGKNLKE PLVYNN
Sbjct: 301 NSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLYFGKNLKETPLVYNN 360

Query: 374 TAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLAGGAGMILINTILEGE 433
           TAGDG+ETNFCT GSLDPTMV+GKI VCERGTNSRT+KGEQVKLAGGAGMILINTILEGE
Sbjct: 361 TAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAGGAGMILINTILEGE 420

Query: 434 DLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYGSRAPRVAAFSSRGPS 493
           DLLADSHVLPATSVG SA KSILNYIASSKRQAKASI+FKGTKYGSRAPRVAAFSSRGPS
Sbjct: 421 DLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGSRAPRVAAFSSRGPS 480

Query: 494 FFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALV 553
           F+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAAL+
Sbjct: 481 FYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALL 540

Query: 554 KSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFAFGSGHVDPEKASHPG 613
           KSVHKDWSPAAIKSALMTTAYVTDNK HLISDVGRASGGPAD FAFGSGHVDPEKAS PG
Sbjct: 541 KSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAFGSGHVDPEKASDPG 600

Query: 614 LIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGDLNYPSFSVFMKKGKN 673
           L+YDIAPQDYITYLCSLKY STQI+LVSRGKFTCSSK TF QPGDLNYPSFSVFMKKGKN
Sbjct: 601 LVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDLNYPSFSVFMKKGKN 660

Query: 674 VNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLGEKLSYKVSFYALGKRE 733
           VNSTFKRTVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+FVKLGEKLSYKVSF ALGKR+
Sbjct: 661 VNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGEKLSYKVSFVALGKRK 720

Query: 734 SLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           SLD+FSFGSLVWHSGTY VRSPIAVTWQ
Sbjct: 721 SLDDFSFGSLVWHSGTYVVRSPIAVTWQ 743

BLAST of CsGy5G010310 vs. NCBI nr
Match: XP_038891850.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038891851.1 subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038891852.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1286 bits (3329), Expect = 0.0
Identity = 646/773 (83.57%), Postives = 705/773 (91.20%), Query Frame = 0

Query: 1   MGFKEVLLLLYITM--LTTSSVAMDQQTYIVHMDTTKMDTPN-PEQWYTAIIDSVNQLSS 60
           M  +++L+ L IT+  L TSS A+DQQ+YI+HMDT+KM T N PEQWYT +IDSVN+L+S
Sbjct: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60

Query: 61  LYGDNNDDEEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTT 120
           L  DNN+ EEA  AAEILYVYKT        LS+++LHSLSK+PGF+AATPN+LLQLHTT
Sbjct: 61  LDNDNNE-EEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTT 120

Query: 121 HSPQFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGP 180
           HSPQFLGL+RG GLWNSSNLASDIIIG+LDTGIWPEHISFQDKGL PVP+KWKGICQ GP
Sbjct: 121 HSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGP 180

Query: 181 NFSHSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRAS 240
            FS SNCNKKLIGAR FIQ YEA VGRLN TG FRS RDS+GHGTHTASTAAGNF+NRAS
Sbjct: 181 KFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRAS 240

Query: 241 FYNQGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSII 300
           FYNQ +G ATGMRFTSRIA+YKVCWPEGCASADILAAMDHA+ADGVDVLSISLGGGS I 
Sbjct: 241 FYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIF 300

Query: 301 YSDQIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGN 360
           YSD+IAIAAFGAIQ+GVFVSCSAGNSGP+ISTV NVAPW+MTVAASYTDRTFPTTV+LGN
Sbjct: 301 YSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGN 360

Query: 361 GKVFEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRT 420
           GKVFEGSS YFGKN+ E+PLVYNNTAGDG+E+N CTAGSL P+MV+GKIVVCERGTNSRT
Sbjct: 361 GKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRT 420

Query: 421 KKGEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKAS 480
            KGEQVKLAGGAGMILINT LEGE+L+ADSHVLPAT+VGASA+K+I++YIASSK QAKAS
Sbjct: 421 AKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKAS 480

Query: 481 IIFKGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 540
           I FKGTKYGS+APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR
Sbjct: 481 ITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 540

Query: 541 RVLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRA 600
           RVLFNIISGTSMSCPHVSGLAAL+KS HKDWSPAAIKSALMTTAYVTDNK HLISDV RA
Sbjct: 541 RVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRA 600

Query: 601 SGGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSS 660
           SGGPAD +AFGSGHVDPEKAS+PGL+YDIAPQDYI YLCSLKY S QI+LVSRGKFTCSS
Sbjct: 601 SGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSS 660

Query: 661 KNTFSQPGDLNYPSFSVFMKK-GKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKP 720
           K  F QPGDLNYPSFS+FMKK  KNV  TFKRTVTNVGIPRSDYT +INNP+GIR+IVKP
Sbjct: 661 KRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKP 720

Query: 721 EKLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           EKL+FV+LG KLSYKVSF ALGKRE+LD+FSFGSLVW SG YAVRSPIAVTWQ
Sbjct: 721 EKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 772

BLAST of CsGy5G010310 vs. NCBI nr
Match: TYK29019.1 (subtilisin-like protease SBT1.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 640/681 (93.98%), Postives = 659/681 (96.77%), Query Frame = 0

Query: 81  LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGHGLWNSSNLASDIIIGVLDT 140
           LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP+FLGLQRGHGLWNSSNLASDIIIGVLDT
Sbjct: 142 LSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQRGHGLWNSSNLASDIIIGVLDT 201

Query: 141 GIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLIGARTFIQAYEAAVGRLNGT 200
           GIWPEHISFQDK LPPVPSKWKGICQTGP+FS SNCNKKLIGARTFIQAYEAAVGRLN T
Sbjct: 202 GIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKKLIGARTFIQAYEAAVGRLNAT 261

Query: 201 GIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATGMRFTSRIASYKVCWPEGCAS 260
           G FRSARDS+GHGTHTASTAAGNF+NRASFYNQGMGVATGMRFTSRIA+YKVCWPEGCAS
Sbjct: 262 GTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVATGMRFTSRIAAYKVCWPEGCAS 321

Query: 261 ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS 320
           ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS
Sbjct: 322 ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS 381

Query: 321 TVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYFGKNLKEVPLVYNNTAGDGQE 380
           TV NVAPWVMTVAASYTDRTFPTTVRLGNG VFEGSS YFGKNLKE PLVYNNTAGDG+E
Sbjct: 382 TVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLYFGKNLKETPLVYNNTAGDGRE 441

Query: 381 TNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLAGGAGMILINTILEGEDLLADSH 440
           TNFCT GSLDPTMV+GKI VCERGTNSRT+KGEQVKLAGGAGMILINTILEGEDLLADSH
Sbjct: 442 TNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAGGAGMILINTILEGEDLLADSH 501

Query: 441 VLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYGSRAPRVAAFSSRGPSFFKPYVI 500
           VLPATSVG SA KSILNYIASSKRQAKASI+FKGTKYGSRAPRVAAFSSRGPSF+KPYVI
Sbjct: 502 VLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGSRAPRVAAFSSRGPSFYKPYVI 561

Query: 501 KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALVKSVHKDW 560
           KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAAL+KSVHKDW
Sbjct: 562 KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSVHKDW 621

Query: 561 SPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFAFGSGHVDPEKASHPGLIYDIAP 620
           SPAAIKSALMTTAYVTDNK HLISDVGRASGGPAD FAFGSGHVDPEKAS PGL+YDIAP
Sbjct: 622 SPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAFGSGHVDPEKASDPGLVYDIAP 681

Query: 621 QDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGDLNYPSFSVFMKKGKNVNSTFKR 680
           QDYITYLCSLKY STQI+LVSRGKFTCSSK TF QPGDLNYPSFSVFMKKGKNVNSTFKR
Sbjct: 682 QDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDLNYPSFSVFMKKGKNVNSTFKR 741

Query: 681 TVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLGEKLSYKVSFYALGKRESLDEFSF 740
           TVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+FVKLGEKLSYKVSF ALGKR+SLD+FSF
Sbjct: 742 TVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGEKLSYKVSFVALGKRKSLDDFSF 801

Query: 741 GSLVWHSGTYAVRSPIAVTWQ 761
           GSLVWHSGTY VRSPIAVTWQ
Sbjct: 802 GSLVWHSGTYVVRSPIAVTWQ 822

BLAST of CsGy5G010310 vs. ExPASy TrEMBL
Match: A0A1S3BZC2 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495085 PE=3 SV=1)

HSP 1 Score: 1396 bits (3613), Expect = 0.0
Identity = 706/769 (91.81%), Postives = 730/769 (94.93%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           MGFKEVLL LYITMLTTS VAMDQQ+YIVHMDTTKM T NPEQWYTAII SVN+LSSL  
Sbjct: 1   MGFKEVLLFLYITMLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDA 60

Query: 61  DNNDDEEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120
           +NN+ E+ALN AEILYVYKT        LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP
Sbjct: 61  NNNE-EQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTHSP 120

Query: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180
           +FLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDK LPPVPSKWKGICQTGP+FS
Sbjct: 121 KFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFS 180

Query: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240
            SNCNKKLIGARTFIQAYEAAVGRLN TG FRSARDS+GHGTHTASTAAGNF+NRASFYN
Sbjct: 181 LSNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYN 240

Query: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300
           QGMGVATGMRFTSRIA+YKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD
Sbjct: 241 QGMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300

Query: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360
           QIAIAAFGAIQKGVFVSCSAGNSGPFISTV NVAPWVMTVAASYTDRTFPTTVRLGNG V
Sbjct: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMV 360

Query: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420
           FEGSS YFGKNLKE PLVYNNTAGDG+ETNFCT GSLDPTMV+GKI VCERGTNSRT+KG
Sbjct: 361 FEGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKG 420

Query: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480
           EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVG SA KSILNYIASSKRQAKASI+F
Sbjct: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMF 480

Query: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540
           KGTKYGSRAPRVAAFSSRGPSF+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL
Sbjct: 481 KGTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540

Query: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600
           FNIISGTSMSCPHVSGLAAL+KSVHKDWSPAAIKSALMTTAYVTDNK HLISDVGRASGG
Sbjct: 541 FNIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGG 600

Query: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660
           PAD FAFGSGHVDPEKAS PGL+YDIAPQDYITYLCSLKY STQI+LVSRGKFTCSSK T
Sbjct: 601 PADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRT 660

Query: 661 FSQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLN 720
           F QPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+
Sbjct: 661 FFQPGDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLS 720

Query: 721 FVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           FVKLGEKLSYKVSF ALGKR+SLD+FSFGSLVWHSGTY VRSPIAVTWQ
Sbjct: 721 FVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of CsGy5G010310 vs. ExPASy TrEMBL
Match: A0A5A7UKU0 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002890 PE=3 SV=1)

HSP 1 Score: 1368 bits (3542), Expect = 0.0
Identity = 690/748 (92.25%), Postives = 714/748 (95.45%), Query Frame = 0

Query: 14  MLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYGDNNDDEEALNAAE 73
           MLTTS VAMDQQ+YIVHMDTTKM T NPEQWYTAII SVN+LSSL  +NN+ E+ALN AE
Sbjct: 1   MLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDANNNE-EQALNIAE 60

Query: 74  ILYVYKTLSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGHGLWNSSNLASDI 133
           ILY    LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP+FLGLQRGHGLWNSSNLASDI
Sbjct: 61  ILY----LSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQRGHGLWNSSNLASDI 120

Query: 134 IIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLIGARTFIQAYEAA 193
           IIGVLDTGIWPEHISFQDK LPPVPSKWKGICQTGP+FS SNCNKKLIGARTFIQAYEAA
Sbjct: 121 IIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKKLIGARTFIQAYEAA 180

Query: 194 VGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATGMRFTSRIASYKVC 253
           VGRLN TG FRSARDS+GHGTHTASTAAGNF+NRASFYNQGMGVATGMRFTSRIA+YKVC
Sbjct: 181 VGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVATGMRFTSRIAAYKVC 240

Query: 254 WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG 313
           WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG
Sbjct: 241 WPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAG 300

Query: 314 NSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYFGKNLKEVPLVYNN 373
           NSGPFISTV NVAPWVMTVAASYTDRTFPTTVRLGNG VFEGSS YFGKNLKE PLVYNN
Sbjct: 301 NSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLYFGKNLKETPLVYNN 360

Query: 374 TAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLAGGAGMILINTILEGE 433
           TAGDG+ETNFCT GSLDPTMV+GKI VCERGTNSRT+KGEQVKLAGGAGMILINTILEGE
Sbjct: 361 TAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAGGAGMILINTILEGE 420

Query: 434 DLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYGSRAPRVAAFSSRGPS 493
           DLLADSHVLPATSVG SA KSILNYIASSKRQAKASI+FKGTKYGSRAPRVAAFSSRGPS
Sbjct: 421 DLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGSRAPRVAAFSSRGPS 480

Query: 494 FFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALV 553
           F+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAAL+
Sbjct: 481 FYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALL 540

Query: 554 KSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFAFGSGHVDPEKASHPG 613
           KSVHKDWSPAAIKSALMTTAYVTDNK HLISDVGRASGGPAD FAFGSGHVDPEKAS PG
Sbjct: 541 KSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAFGSGHVDPEKASDPG 600

Query: 614 LIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGDLNYPSFSVFMKKGKN 673
           L+YDIAPQDYITYLCSLKY STQI+LVSRGKFTCSSK TF QPGDLNYPSFSVFMKKGKN
Sbjct: 601 LVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDLNYPSFSVFMKKGKN 660

Query: 674 VNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLGEKLSYKVSFYALGKRE 733
           VNSTFKRTVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+FVKLGEKLSYKVSF ALGKR+
Sbjct: 661 VNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGEKLSYKVSFVALGKRK 720

Query: 734 SLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           SLD+FSFGSLVWHSGTY VRSPIAVTWQ
Sbjct: 721 SLDDFSFGSLVWHSGTYVVRSPIAVTWQ 743

BLAST of CsGy5G010310 vs. ExPASy TrEMBL
Match: A0A5D3E023 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001300 PE=3 SV=1)

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 640/681 (93.98%), Postives = 659/681 (96.77%), Query Frame = 0

Query: 81  LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGHGLWNSSNLASDIIIGVLDT 140
           LSS+NLH LSKVPGF+AA+PNELLQLHTTHSP+FLGLQRGHGLWNSSNLASDIIIGVLDT
Sbjct: 142 LSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQRGHGLWNSSNLASDIIIGVLDT 201

Query: 141 GIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLIGARTFIQAYEAAVGRLNGT 200
           GIWPEHISFQDK LPPVPSKWKGICQTGP+FS SNCNKKLIGARTFIQAYEAAVGRLN T
Sbjct: 202 GIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKKLIGARTFIQAYEAAVGRLNAT 261

Query: 201 GIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATGMRFTSRIASYKVCWPEGCAS 260
           G FRSARDS+GHGTHTASTAAGNF+NRASFYNQGMGVATGMRFTSRIA+YKVCWPEGCAS
Sbjct: 262 GTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVATGMRFTSRIAAYKVCWPEGCAS 321

Query: 261 ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS 320
           ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS
Sbjct: 322 ADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPFIS 381

Query: 321 TVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYFGKNLKEVPLVYNNTAGDGQE 380
           TV NVAPWVMTVAASYTDRTFPTTVRLGNG VFEGSS YFGKNLKE PLVYNNTAGDG+E
Sbjct: 382 TVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLYFGKNLKETPLVYNNTAGDGRE 441

Query: 381 TNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLAGGAGMILINTILEGEDLLADSH 440
           TNFCT GSLDPTMV+GKI VCERGTNSRT+KGEQVKLAGGAGMILINTILEGEDLLADSH
Sbjct: 442 TNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAGGAGMILINTILEGEDLLADSH 501

Query: 441 VLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYGSRAPRVAAFSSRGPSFFKPYVI 500
           VLPATSVG SA KSILNYIASSKRQAKASI+FKGTKYGSRAPRVAAFSSRGPSF+KPYVI
Sbjct: 502 VLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGSRAPRVAAFSSRGPSFYKPYVI 561

Query: 501 KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALVKSVHKDW 560
           KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAAL+KSVHKDW
Sbjct: 562 KPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSVHKDW 621

Query: 561 SPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFAFGSGHVDPEKASHPGLIYDIAP 620
           SPAAIKSALMTTAYVTDNK HLISDVGRASGGPAD FAFGSGHVDPEKAS PGL+YDIAP
Sbjct: 622 SPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAFGSGHVDPEKASDPGLVYDIAP 681

Query: 621 QDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGDLNYPSFSVFMKKGKNVNSTFKR 680
           QDYITYLCSLKY STQI+LVSRGKFTCSSK TF QPGDLNYPSFSVFMKKGKNVNSTFKR
Sbjct: 682 QDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDLNYPSFSVFMKKGKNVNSTFKR 741

Query: 681 TVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLGEKLSYKVSFYALGKRESLDEFSF 740
           TVTNVGIPRSDYTVRI NPKGIRIIVKPEKL+FVKLGEKLSYKVSF ALGKR+SLD+FSF
Sbjct: 742 TVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGEKLSYKVSFVALGKRKSLDDFSF 801

Query: 741 GSLVWHSGTYAVRSPIAVTWQ 761
           GSLVWHSGTY VRSPIAVTWQ
Sbjct: 802 GSLVWHSGTYVVRSPIAVTWQ 822

BLAST of CsGy5G010310 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1200 bits (3104), Expect = 0.0
Identity = 609/770 (79.09%), Postives = 669/770 (86.88%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           MGF+EV +L    ML  SS  +DQQTYI+HMDTTKM TPNPEQWYT IIDSVN+LSSL  
Sbjct: 1   MGFREVWVLSI--MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSL-- 60

Query: 61  DNNDDEEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSP 120
             +D+EEA NAAEILYVYKT        L+S+ LHSLSK+PGF+AATPNELLQLHTTHSP
Sbjct: 61  --DDNEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSP 120

Query: 121 QFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFS 180
           QFLGLQR HGLWN SNLASDIIIG+LDTGIWPEHISFQDKGL  VP KWKGICQTGP FS
Sbjct: 121 QFLGLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFS 180

Query: 181 HSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYN 240
            SNCNKKLIGA  +I+ YEA VGRLN TG FRS RDS+GHGTHTASTAAG+ ++ ASFYN
Sbjct: 181 SSNCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYN 240

Query: 241 QGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300
           QGMGVA+GMRFTSRI +YKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGGSS  Y D
Sbjct: 241 QGMGVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKD 300

Query: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKV 360
            IAIAAFGAIQKGVFVSCSAGNSGP  STV N APW+MTVAASYTDRTFPTTV+LGNG+V
Sbjct: 301 NIAIAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQV 360

Query: 361 FEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKG 420
           FEGSS Y+GK++ E+PLVYNNTAGDGQETN C AGSLDP+MV+GKIV+CERGT SRT+KG
Sbjct: 361 FEGSSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKG 420

Query: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIF 480
           EQVKLAGG GMILINT  EGE+L AD HVLPAT++GASA K+IL+YIASSK QAKASI+F
Sbjct: 421 EQVKLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVF 480

Query: 481 KGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 540
           +GTKYGS+APRVAAFSSRGPS   P VIKPD+TAPGVNILAAWPPIVSPSEL SD RRV+
Sbjct: 481 EGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVM 540

Query: 541 FNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGG 600
           FNIISGTSMSCPHVSGLAAL+KS H DWSPAAIKSALMTTAYVTD+K  LISDVG+A+G 
Sbjct: 541 FNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGE 600

Query: 601 PADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNT 660
           PA  F FGSGHVDPEKAS PGLIYDI PQDYI YLCSLKY S+QI+LVSRG  TCSSK T
Sbjct: 601 PATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRT 660

Query: 661 FSQPGDLNYPSFSVFMKK-GKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKL 720
             +PGDLNYPSFSVFMKK  K V+ T KRTVTNVGI RSDYTV+INNPKG+ +IVKPEKL
Sbjct: 661 VVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKL 720

Query: 721 NFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           +F  LGE+LSYKVSF +LG +E+LD+FSFGSLVW SG YAVRSPI VTWQ
Sbjct: 721 SFGSLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of CsGy5G010310 vs. ExPASy TrEMBL
Match: A0A5D3DZC9 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1196 bits (3093), Expect = 0.0
Identity = 603/757 (79.66%), Postives = 661/757 (87.32%), Query Frame = 0

Query: 14  MLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYGDNNDDEEALNAAE 73
           ML  SS  +DQQTYI+HMDTTKM TPNPEQWYT IIDSVN+LSSL    +D+EEA NAAE
Sbjct: 1   MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSL----DDNEEASNAAE 60

Query: 74  ILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGHGLWN 133
           ILYVYKT        L+S+ LHSLSK+PGF+AATPNELLQLHTTHSPQFLGLQR HGLWN
Sbjct: 61  ILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWN 120

Query: 134 SSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLIGART 193
            SNLASDIIIG+LDTGIWPEHISFQDKGL  VP KWKGICQTGP FS SNCNKKLIGA  
Sbjct: 121 FSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASA 180

Query: 194 FIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATGMRFTS 253
           +I+ YEA VGRLN TG FRS RDS+GHGTHTASTAAG+ ++ ASFYNQGMGVA+GMRFTS
Sbjct: 181 YIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTS 240

Query: 254 RIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKG 313
           RI +YKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGGSS  Y D IAIAAFGAIQKG
Sbjct: 241 RIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKG 300

Query: 314 VFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYFGKNLK 373
           VFVSCSAGNSGP  STV N APW+MTVAASYTDRTFPTTV+LGNG+VFEGSS Y+GK++ 
Sbjct: 301 VFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSIN 360

Query: 374 EVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLAGGAGMIL 433
           E+PLVYNNTAGDGQETN C AGSLDP+MV+GKIV+CERGT SRT+KGEQVKLAGG GMIL
Sbjct: 361 ELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMIL 420

Query: 434 INTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYGSRAPRVA 493
           INT  EGE+L AD HVLPAT++GASA K+IL+YIASSK QAKASI+F+GTKYGS+APRVA
Sbjct: 421 INTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQAPRVA 480

Query: 494 AFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPH 553
           AFSSRGPS   P VIKPD+TAPGVNILAAWPPIVSPSEL SD RRV+FNIISGTSMSCPH
Sbjct: 481 AFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPH 540

Query: 554 VSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFAFGSGHVD 613
           VSGLAAL+KS H DWSPAAIKSALMTTAYVTD+K  LISDVG+A+G PA  F FGSGHVD
Sbjct: 541 VSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVD 600

Query: 614 PEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGDLNYPSFS 673
           PEKAS PGLIYDI PQDYI YLCSLKY S+QI+LVSRG  TCSSK T  +PGDLNYPSFS
Sbjct: 601 PEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFS 660

Query: 674 VFMKK-GKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLGEKLSYKV 733
           VFMKK  K V+ T KRTVTNVGI RSDYTV+INNPKG+ +IVKPEKL+F  LGE+LSYKV
Sbjct: 661 VFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKV 720

Query: 734 SFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 761
           SF +LG +E+LD+FSFGSLVW SG YAVRSPI VTWQ
Sbjct: 721 SFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 753

BLAST of CsGy5G010310 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 798.1 bits (2060), Expect = 6.1e-231
Identity = 420/772 (54.40%), Postives = 538/772 (69.69%), Query Frame = 0

Query: 1   MGFKEVLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYG 60
           M F    ++ +  +   S+V+  +QTY++H  TT             I+ S+   +SL  
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTST---------KHIVTSL--FNSLQT 77

Query: 61  DN-NDDEEALNAAEILYVYK--------TLSSRNLHSLSKVPGFVAATPNELLQLHTTHS 120
           +N NDD+ +L   EI Y+Y+        TL+   L ++    GF++A P+ELL LHTT+S
Sbjct: 78  ENINDDDFSL--PEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYS 137

Query: 121 PQFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNF 180
            +FLGL+ G GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVPS+W+G C  G NF
Sbjct: 138 HEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNF 197

Query: 181 SHSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFY 240
           S S CNKK+IGA  F + YE+ VG++N T  FRS RD+ GHGTHTASTAAG+ + +A+++
Sbjct: 198 SSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYF 257

Query: 241 NQGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYS 300
            Q  G+A+GMRFTSRIA+YK CW  GCAS D++AA+D A+ DGVDV+S+SLGG S   Y 
Sbjct: 258 GQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYV 317

Query: 301 DQIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGK 360
           D IAIA FGA+QK +FVSCSAGNSGP  STVSN APW+MTVAASYTDRTFP  VR+GN K
Sbjct: 318 DPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRK 377

Query: 361 VFEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 420
              GSS Y GK+LK +PL +N TAG+     FC   SL   +V GKIV+C RG + RT K
Sbjct: 378 SLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAK 437

Query: 421 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASII 480
           GE+VK +GGA M+L++T  EGE+LLAD HVLPA S+G S  K++LNY+A +   A AS+ 
Sbjct: 438 GEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVR 497

Query: 481 FKGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRV 540
           F+GT YG+ AP VAAFSSRGPS   P + KPDI APG+NILA W P  SPS L SD RRV
Sbjct: 498 FRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRV 557

Query: 541 LFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASG 600
            FNIISGTSM+CPH+SG+AAL+KSVH DWSPA IKSA+MTTA +TDN+   I D G A  
Sbjct: 558 QFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGA 617

Query: 601 -GPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSK 660
              A +FAFG+G+VDP +A  PGL+YD +  DY+ YLCSL YTS +I L S   +TC+S 
Sbjct: 618 ESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASN 677

Query: 661 NTFSQPGDLNYPSFSVFMKKGKNVNST-FKRTVTNVGIPRSDYTVRINNPKGIRIIVKPE 720
                PGDLNYPSF+V +  G N+ +  +KRTVTNVG P  +Y V +  PKG+++ V+P+
Sbjct: 678 AVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPK 737

Query: 721 KLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 762
            L F K  E+LSY V++ A   R S    SFG LVW    Y VRSPIAVTW+
Sbjct: 738 VLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of CsGy5G010310 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 666.4 bits (1718), Expect = 2.8e-191
Identity = 363/771 (47.08%), Postives = 490/771 (63.55%), Query Frame = 0

Query: 9   LLYITMLTTSSVAMDQQTYIVHMDTTKMDTP--NPEQWYTAIIDSVNQLSSLYGDNNDDE 68
           L+++   TT+ ++  ++TY++HMD + M  P  N  QWY++ I+SV Q       +   E
Sbjct: 20  LIFLQAETTTQIS-TKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQ-------HKSQE 79

Query: 69  EALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQ 128
           E  N   ILY Y+T        L+      L +  G VA  P    +LHTT SP FLGL+
Sbjct: 80  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 139

Query: 129 R--GHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 188
           R     +W       D+++GVLDTGIWPE  SF D G+ PVP+ W+G C+TG  F   NC
Sbjct: 140 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 199

Query: 189 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 248
           N+K++GAR F + YEAA G+++    ++S RD +GHGTHTA+T AG+ +  A+ +    G
Sbjct: 200 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 259

Query: 249 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 308
            A GM   +R+A+YKVCW  GC S+DIL+A+D AVADGV VLSISLGGG S    D ++I
Sbjct: 260 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 319

Query: 309 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 368
           A FGA++ GVFVSCSAGN GP   +++NV+PW+ TV AS  DR FP TV++G  + F+G 
Sbjct: 320 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 379

Query: 369 SSYFGKNL----KEVPLVY-NNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 428
           S Y G+ +    K+ PLVY    A     T+FC  G+LD   V GKIV+C+RG   R +K
Sbjct: 380 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 439

Query: 429 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASII 488
           G+ VK AGG GM+L NT   GE+L+ADSH+LPA +VG    K I  Y  +SK+ A AS+ 
Sbjct: 440 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK-ATASLE 499

Query: 489 FKGTKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 548
             GT+ G + +P VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++PS L SD RR
Sbjct: 500 ILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRR 559

Query: 549 VLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRAS 608
           V FNI+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAYV DN    ++D   A+
Sbjct: 560 VKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA 619

Query: 609 GGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSK 668
             P+  +  G+GH+DP +A+ PGL+YDI PQ+Y  +LC+   + +Q+ + ++        
Sbjct: 620 --PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKH 679

Query: 669 NTFSQPGDLNYPSFSVFMKKGKNVNS-TFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPE 728
                PG+LNYP+ S    +  +V + T +RTVTNVG   S Y V ++  KG  + V+P+
Sbjct: 680 TLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 729 KLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
            LNF    +KLSY V+F     R  +    FG LVW S T+ VRSP+ +TW
Sbjct: 740 TLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of CsGy5G010310 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 660.2 bits (1702), Expect = 2.0e-189
Identity = 371/780 (47.56%), Postives = 485/780 (62.18%), Query Frame = 0

Query: 9   LLYITMLTTSSVAMDQQTYIVHMDTTKMDT--PNPEQWYTAIIDSVNQLSSLYGDNNDDE 68
           LL ++  ++S+ + +  TYIVH+D     +  P    WYT+ + S+              
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS------------ 69

Query: 69  EALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQ 128
              +   I++ Y T        L+S++   L   P  ++  P ++  LHTT SP+FLGL+
Sbjct: 70  ---SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLR 129

Query: 129 RGH--GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 188
                GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP KWKG C    +F  S C
Sbjct: 130 STDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESAC 189

Query: 189 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 248
           N+KL+GAR F   YEA  G++N T  FRS RDS+GHGTHTAS +AG ++  AS      G
Sbjct: 190 NRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 249

Query: 249 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 308
           VA GM   +R+A+YKVCW  GC  +DILAA D AVADGVDV+S+S+GG     Y D IAI
Sbjct: 250 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 309

Query: 309 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 368
            AFGAI +G+FVS SAGN GP   TV+NVAPW+ TV A   DR FP  V+LGNGK+  G 
Sbjct: 310 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 369

Query: 369 SSYFGKNL---KEVPLVYNNT--AGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKK 428
           S Y G  L   +  PLVY  +   GDG  ++ C  GSLDP +V+GKIV+C+RG NSR  K
Sbjct: 370 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 429

Query: 429 GEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAK---- 488
           GE V+  GG GMI+ N + +GE L+AD HVLPATSVGAS    I  YI+ S +       
Sbjct: 430 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHP 489

Query: 489 -ASIIFKGTKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELE 548
            A+I+FKGT+ G R AP VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + PS + 
Sbjct: 490 TATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVT 549

Query: 549 SDKRRVLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISD 608
           SD RR  FNI+SGTSM+CPHVSGLAAL+K+ H DWSPAAI+SAL+TTAY  DN    + D
Sbjct: 550 SDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD 609

Query: 609 VGRASGGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKF 668
              ++G  +    +GSGHV P KA  PGL+YDI   DYI +LC+  YT T I  ++R + 
Sbjct: 610 --ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 669

Query: 669 TCSSKNTFSQPGDLNYPSFS-VFMKKGKNVNST-FKRTVTNVGIPRSDYTVRINNPKGIR 728
            C         G+LNYPSFS VF + G++  ST F RTVTNVG   S Y ++I  P+G  
Sbjct: 670 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 729

Query: 729 IIVKPEKLNFVKLGEKLSY--KVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 762
           + V+PEKL+F ++G+KLS+  +V    +           G +VW  G   V SP+ VT Q
Sbjct: 730 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of CsGy5G010310 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 646.7 bits (1667), Expect = 2.3e-185
Identity = 365/772 (47.28%), Postives = 486/772 (62.95%), Query Frame = 0

Query: 8   LLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNP--EQWYTAIIDSVNQLSSLYGDNNDD 67
           LLL +     SS + DQ TYIVHM  ++M +       WY + + S++            
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS------------ 72

Query: 68  EEALNAAEILYVYKT--------LSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGL 127
               ++AE+LY Y+         L+     SL   PG ++  P    +LHTT +P FLGL
Sbjct: 73  ----DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 132

Query: 128 -QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNC 187
            +    L+  +   SD+++GVLDTG+WPE  S+ D+G  P+PS WKG C+ G NF+ S C
Sbjct: 133 DEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 192

Query: 188 NKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMG 247
           N+KLIGAR F + YE+ +G ++ +   RS RD +GHGTHT+STAAG+ +  AS      G
Sbjct: 193 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 252

Query: 248 VATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAI 307
            A GM   +R+A YKVCW  GC S+DILAA+D A+AD V+VLS+SLGGG S  Y D +AI
Sbjct: 253 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 312

Query: 308 AAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGS 367
            AF A+++G+ VSCSAGN+GP  S++SNVAPW+ TV A   DR FP    LGNGK F G 
Sbjct: 313 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 372

Query: 368 SSYFGKNL--KEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQ 427
           S + G+ L  K +P +Y   A +    N C  G+L P  V+GKIV+C+RG N+R +KG+ 
Sbjct: 373 SLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDV 432

Query: 428 VKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKG 487
           VK AGG GMIL NT   GE+L+AD+H+LPAT+VG  A   I +Y+ +      ASI   G
Sbjct: 433 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT-ASISILG 492

Query: 488 TKYGSR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLF 547
           T  G + +P VAAFSSRGP+   P ++KPD+ APGVNILAAW     P+ L SD RRV F
Sbjct: 493 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 552

Query: 548 NIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGP 607
           NIISGTSMSCPHVSGLAAL+KSVH +WSPAAI+SALMTTAY T      + D+  A+G P
Sbjct: 553 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKP 612

Query: 608 ADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTF 667
           +  F  G+GHV P  A++PGLIYD+  +DY+ +LC+L YTS QI  VSR  +TC    ++
Sbjct: 613 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 672

Query: 668 SQPGDLNYPSFSVFMKKGKNVNST----FKRTVTNVGIPRSDYTVRI-NNPKGIRIIVKP 727
           S   DLNYPSF+V      NV+      + RTVT+VG     Y+V++ +   G++I V+P
Sbjct: 673 S-VADLNYPSFAV------NVDGVGAYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEP 732

Query: 728 EKLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
             LNF +  EK SY V+F     + S    SFGS+ W  G + V SP+A++W
Sbjct: 733 AVLNFKEANEKKSYTVTFTVDSSKPS-GSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of CsGy5G010310 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.5e-181
Identity = 358/764 (46.86%), Postives = 479/764 (62.70%), Query Frame = 0

Query: 6   VLLLLYITMLTTSSVAMDQQTYIVHMDTTKMDTPNP----EQWYTAIIDSVNQLSSLYGD 65
           +   L++ + TT+     ++TYI+ ++ +  D P        WYT+ ++S + L   Y  
Sbjct: 14  ITTFLFLLLHTTA-----KKTYIIRVNHS--DKPESFLTHHDWYTSQLNSESSLLYTYTT 73

Query: 66  NNDDEEALNAAEILYVYKTLSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQRGH 125
           +     A       Y+  T +   L S + +   +    + L  LHTT +P+FLGL    
Sbjct: 74  SFHGFSA-------YLDSTEADSLLSSSNSI---LDIFEDPLYTLHTTRTPEFLGLNSEF 133

Query: 126 GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNKKLI 185
           G+ +  + ++ +IIGVLDTG+WPE  SF D  +P +PSKWKG C++G +F    CNKKLI
Sbjct: 134 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 193

Query: 186 GARTFIQAYE-AAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVATG 245
           GAR+F + ++ A+ G  +      S RD +GHGTHT++TAAG+ +  ASF     G A G
Sbjct: 194 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 253

Query: 246 MRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFG 305
           M   +R+A+YKVCW  GC  +DILAAMD A+ DGVDVLS+SLGGGS+  Y D IAI AF 
Sbjct: 254 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 313

Query: 306 AIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSSYF 365
           A+++GVFVSCSAGNSGP  ++V+NVAPWVMTV A   DR FP    LGNGK   G S Y 
Sbjct: 314 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 373

Query: 366 GKNL--KEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLA 425
           G  +  K + LVYN   G+   +N C  GSLD ++VRGKIVVC+RG N+R +KG  V+ A
Sbjct: 374 GVGMGTKPLELVYNK--GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 433

Query: 426 GGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYG 485
           GG GMI+ NT   GE+L+ADSH+LPA +VG      +  Y+ S  +   A ++FKGT   
Sbjct: 434 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPT-ALLVFKGTVLD 493

Query: 486 SR-APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIIS 545
            + +P VAAFSSRGP+   P ++KPD+  PGVNILA W   + P+ L+ D RR  FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMS 553

Query: 546 GTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSF 605
           GTSMSCPH+SGLA L+K+ H +WSP+AIKSALMTTAYV DN    + D   A    ++ +
Sbjct: 554 GTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSNPY 613

Query: 606 AFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQI-SLVSRGKFTCSSKNTFSQP 665
           A GSGHVDP+KA  PGL+YDI+ ++YI +LCSL YT   I ++V R    CS K  FS P
Sbjct: 614 AHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDP 673

Query: 666 GDLNYPSFSVFMKKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKL 725
           G LNYPSFSV    GK V   + R VTNVG   S Y V +N    + I VKP KL+F  +
Sbjct: 674 GQLNYPSFSVLF-GGKRV-VRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSV 733

Query: 726 GEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTW 761
           GEK  Y V+F +       ++  FGS+ W +  + VRSP+A +W
Sbjct: 734 GEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751