Homology
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match:
O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)
HSP 1 Score: 426.0 bits (1094), Expect = 5.8e-118
Identity = 213/463 (46.00%), Postives = 306/463 (66.09%), Query Frame = 0
Query: 37 LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
LPPGP PWPI+GNL Q+G+ H + A YG + +LK+G + +VVAS+ + A+ +LKT
Sbjct: 28 LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87
Query: 97 HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
HDR LS RY+ Q + + ++S+V+S +C + WKNLR +C+ LFT K IESQA +RE
Sbjct: 88 HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 147
Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGD---GFNGIKGSFK 216
KK +E+VE+L KQG VKI + +F ++ N NLIFS ++ + +GD G + K
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFE-LGDPNSGSSEFKEYLW 207
Query: 217 KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQG-SAS 276
+M++LG + N AD++P+L +FDL G RK+ A + + ++WG +++ER+ G +
Sbjct: 208 RMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSK 267
Query: 277 SDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKV 336
+DF+DV + SG +D QIN L+ EL AGT+T+ +T+EWAM+E+ K+ I K+R EI+ V
Sbjct: 268 NDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTV 327
Query: 337 TKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNV 396
E + ES+ L YL + VKETLRLHPP P LLPR A C ++ Y+IPKD I VN
Sbjct: 328 VGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNA 387
Query: 397 WGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPL 456
WGIGRDP W D TF+PERF ++VDF+G D+ +PFG GRRICPGLP+A + L
Sbjct: 388 WGIGRDPKTWTDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIAL 447
Query: 457 ILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
++AT + N +W LPNG + L + + G+TLQK L ++PK
Sbjct: 448 LVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 484
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match:
Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 424.1 bits (1089), Expect = 2.2e-117
Identity = 228/498 (45.78%), Postives = 323/498 (64.86%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
M + S +I+ S LL +F IL+ + + LPPGP P PI+GNL Q+G H
Sbjct: 1 MEVLSIAIVSFSFLLF---LFFILRDSRPKN-----LPPGPRPSPIVGNLLQLGDKPHAE 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
+ A YG L SLKLG+Q +VVASSPA A +LKTHD+ LS RY+ Q + + ++S
Sbjct: 61 FAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
+V+S EC D+WK LR +C+ LFT K IESQ+ +RE K +E+V+FL K+G VVKI + V
Sbjct: 121 IVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVG--DGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQG 240
F ++ N NLIFS D+ D G +K K++D+G + N AD++P++ + DL G
Sbjct: 181 FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFG 240
Query: 241 LRKKAAIYMNEVDSLWGILIKERREIHCQQGSAS-SDFLDVLIQSGFSDQQINYLINELL 300
R+ A + ++ +WG+++KERR +GS S +DF+DVL+ +G DQ+IN L+ EL
Sbjct: 241 QRRAVAEVLQQIYDVWGVMLKERRG---TKGSESKNDFVDVLLNAGLDDQKINALLMELF 300
Query: 301 SAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETL 360
AGT+T+ +T+EWA++E+ K +++K+R E+ V ++ + ES++ L YL + VKETL
Sbjct: 301 GAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETL 360
Query: 361 RLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGC 420
RLHPP P LLPR A C VM Y+IPK+ I VN W IGRDP W+D F PERF
Sbjct: 361 RLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERF---L 420
Query: 421 GNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLN 480
++VD+KG D+ +PFGGGRRICPGLP+A LI+ATL+ NFEWSLP G S+L ++
Sbjct: 421 SSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMD 480
Query: 481 GRLGVTLQKYEALELIPK 496
+ G+TLQK L ++ K
Sbjct: 481 EKFGLTLQKDPPLLIVLK 482
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match:
Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)
HSP 1 Score: 423.7 bits (1088), Expect = 2.9e-117
Identity = 211/462 (45.67%), Postives = 302/462 (65.37%), Query Frame = 0
Query: 37 LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
LPPGP PWPI+GNL Q+G+ H Q A YG L SLKLG++ +VVAS+P A+ +LKT
Sbjct: 23 LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKT 82
Query: 97 HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
HDR LS RY+ Q + + ++S+V+S EC + WK LR +C+ LFT K IESQA +RE
Sbjct: 83 HDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRE 142
Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNG---IKGSFK 216
K E+VE+L+ G+ VKI + VF ++ N NLIFS ++ +GD +G +K
Sbjct: 143 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFK-LGDESSGSVEMKEHLW 202
Query: 217 KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASS 276
+M++LG + N AD++P L +FDL G K A + + S+WG ++KER+ S +
Sbjct: 203 RMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKN 262
Query: 277 DFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT 336
DF+++L+ SG DQQIN L+ E+ AGT+T+ +T+EWA+SE+ K+ + +R E+ V
Sbjct: 263 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVV 322
Query: 337 KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVW 396
+ P+ ES+I + YL + VKETLRLHP P LLPR A C+V+ Y+IPK+ I VN W
Sbjct: 323 GKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAW 382
Query: 397 GIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLI 456
GIGRDP W D F+PERF +++DFKG D+ +PFG GRRICPG+P+A + LI
Sbjct: 383 GIGRDPKRWTDPLKFSPERF---LNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLI 442
Query: 457 LATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
+++L+ NF+W P G D S+L + + G+TLQK L ++PK
Sbjct: 443 VSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPK 478
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match:
O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 422.9 bits (1086), Expect = 4.9e-117
Identity = 211/462 (45.67%), Postives = 303/462 (65.58%), Query Frame = 0
Query: 37 LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
LPPGP PWPI+GNL Q+G+ H + A YG + +LK+G + +VVAS+ + A+ +LKT
Sbjct: 29 LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 88
Query: 97 HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
HDR LS RY+ Q + + ++S+V+S +C + WKNLR +C+ LFT K IESQA +RE
Sbjct: 89 HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 148
Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDL--LDFVGDGFNGIKGSFKK 216
KK +E+VE+L KQG VKI + +F ++ N NLIFS ++ L G + K +
Sbjct: 149 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWR 208
Query: 217 MMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQG-SASS 276
M++LG + N AD++P+L +FDL G RK+ A + + ++WG +++ER+ G + +
Sbjct: 209 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKN 268
Query: 277 DFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT 336
DF+DV + SG +D QIN L+ EL AGT+T+ +T+EWAM+E+ K+ I K+R E++ V
Sbjct: 269 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVV 328
Query: 337 KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVW 396
E + ES+ L YL + VKETLRLHPP P LLPR A C ++ Y+IPKD I VN W
Sbjct: 329 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 388
Query: 397 GIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLI 456
GIGRDP W D TF+PERF ++VDF+G D+ +PFG GRRICPGLP+A + L+
Sbjct: 389 GIGRDPKTWIDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALL 448
Query: 457 LATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
+AT + N +W LPNG + L + + G+TLQK L ++PK
Sbjct: 449 VATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 485
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match:
A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)
HSP 1 Score: 393.7 bits (1010), Expect = 3.2e-108
Identity = 210/490 (42.86%), Postives = 313/490 (63.88%), Query Frame = 0
Query: 12 SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNA----HISITQFANI 71
+L LIP I + + +K P K LPPGP+PWP++GNLP + N HI++ A
Sbjct: 6 ALFSLIPVILVFILLLK-PKYKN--LPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 65
Query: 72 YGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPEC 131
+GP++ L LG Q V+AS+ A +LKTHDR S R+I +M+ + ++SLV+S +C
Sbjct: 66 HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS-DC 125
Query: 132 GDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNT 191
D+WK LR I + +F+ K +++Q+ +RE+K+ EL++FL SK+G VVKI FVF ++ N
Sbjct: 126 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 185
Query: 192 LSNLIFSMDLLDFVGDGFN--GIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAI 251
L N++FS D+ + GD + GI+ ++M+ +G PN+A+FYP L DLQGL+KK
Sbjct: 186 LGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDE 245
Query: 252 YMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTT 311
V +W +KER+ + S D LDVL+ + F+D QIN L E G++T++
Sbjct: 246 RFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSS 305
Query: 312 TTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPF 371
T+EW ++E++K + KVR+E+ +V S I ES++ +L YL +C+KE +RLHP APF
Sbjct: 306 ATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPF 365
Query: 372 LLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKG 431
LLPR A CEVMGY+IPK+S + VN + IGRDP W+D TF PERF ++VDF G
Sbjct: 366 LLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF---LESDVDFHG 425
Query: 432 YDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQ 491
Y+++PFG GRR C G+P+A +PLI+ +L+HN+++ LP G L +N L +TL
Sbjct: 426 AHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLA 483
Query: 492 KYEALELIPK 496
+L ++PK
Sbjct: 486 IDPSLCVVPK 483
BLAST of CsGy5G005795 vs. NCBI nr
Match:
XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])
HSP 1 Score: 996 bits (2574), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH 360
TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH
Sbjct: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH 360
Query: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 420
PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 420
Query: 421 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 480
VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL
Sbjct: 421 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 480
Query: 481 GVTLQKYEALELIPKRNPI 499
GVTLQKYEALELIPKRNPI
Sbjct: 481 GVTLQKYEALELIPKRNPI 499
BLAST of CsGy5G005795 vs. NCBI nr
Match:
KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])
HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 487/497 (97.99%), Postives = 489/497 (98.39%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELL A
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLFAA 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-IDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNK R+EIKKV KESP IDESNISKLQYLWSCVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 360
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPP PFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN
Sbjct: 361 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
Query: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480
Query: 481 LGVTLQKYEALELIPKR 496
LGVTLQKYE L+LIPKR
Sbjct: 481 LGVTLQKYETLKLIPKR 497
BLAST of CsGy5G005795 vs. NCBI nr
Match:
XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])
HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/501 (90.62%), Postives = 473/501 (94.41%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLL+P IFIILK IKSPS KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDLLDFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420
Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480
Query: 481 RLGVTLQKYEALELIPKRNPI 499
RLGVTLQKYE L+LIPKR I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499
BLAST of CsGy5G005795 vs. NCBI nr
Match:
KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])
HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLL+P IFIILK IKSPS KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDL+DFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420
Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480
Query: 481 RLGVTLQKYEALELIPKRNPI 499
RLGVTLQKYE L+LIPKR I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499
BLAST of CsGy5G005795 vs. NCBI nr
Match:
XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])
HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 451/497 (90.74%), Postives = 466/497 (93.76%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLL+PPIFIILKRIKSPS KKLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 11 MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLIS KLGAQRLVVASSPA ATAVLKT D LS RYI Q+TPDRAL++QHS
Sbjct: 71 ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFS ECGD WK LRSICK NLFTAKAIESQAILREKKMKELVEF+ESKQGSVV+IKDFV
Sbjct: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTS FN LSNLIFSMDLLDFVGDGFNGIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL
Sbjct: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
+K IY NEV+S+WGILIKERREI C++GSAS+DFLDVLIQSGFSDQQI+ LI ELL A
Sbjct: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-IDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNK R+EIKKV KESP IDESNISKLQYLWSCVKETLRL
Sbjct: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPP PFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN
Sbjct: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 430
Query: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490
Query: 481 LGVTLQKYEALELIPKR 496
LGVTLQKYE L+LIPKR
Sbjct: 491 LGVTLQKYETLKLIPKR 507
BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match:
A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)
HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/501 (90.62%), Postives = 473/501 (94.41%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLL+P IFIILK IKSPS KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDLLDFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420
Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480
Query: 481 RLGVTLQKYEALELIPKRNPI 499
RLGVTLQKYE L+LIPKR I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499
BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match:
A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)
HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0
Query: 1 MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
MLIPSFSILLPSLLLL+P IFIILK IKSPS KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1 MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60
Query: 61 ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61 ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120
Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180
Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
FTSVFNTLSNLIFSMDL+DFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240
Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300
Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360
Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420
Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480
Query: 481 RLGVTLQKYEALELIPKRNPI 499
RLGVTLQKYE L+LIPKR I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499
BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match:
A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)
HSP 1 Score: 733 bits (1893), Expect = 6.75e-263
Identity = 370/483 (76.60%), Postives = 414/483 (85.71%), Query Frame = 0
Query: 13 LLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLI 72
LLLL+P IFIIL+ I S S K+ PLPPGP PWPILGNL QIGQNAHIS+TQF++IYGPLI
Sbjct: 9 LLLLLPLIFIILRHISSSS-KQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68
Query: 73 SLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWK 132
SLKLGAQRLVVASSPA A A+LKT DR LS RYI QMTPDR L++ SL+FSPECGD WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 133 NLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLI 192
NLRSIC+A LFTA+AIESQA LR KKM E+VEFL+SKQGSVV+IK+ VF +VFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 193 FSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDS 252
FS DL+ FVGDGFNGIKG FK M+DLGL PNLADFYPLLR FDLQGLRKKAAIY N+VDS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248
Query: 253 LWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAM 312
W ILIKERR IH Q SA+SDFLDVLIQSGF+D+QI+YLI E+LSAGTDTTTTTVEWAM
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308
Query: 313 SEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAP 372
+E+LK++ I+N+VR+E KK IDES +S+L YLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
Query: 373 MDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLP 432
CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+F PERFD GC + D KG+DYR+LP
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--DADLKGHDYRFLP 428
Query: 433 FGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALEL 492
FGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLGVTLQK E L+L
Sbjct: 429 FGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKL 487
Query: 493 IPK 495
+ K
Sbjct: 489 LSK 487
BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match:
A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)
HSP 1 Score: 729 bits (1882), Expect = 3.19e-261
Identity = 365/484 (75.41%), Postives = 414/484 (85.54%), Query Frame = 0
Query: 13 LLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLI 72
LLLL+P IF IL+ I S S K+ PLPPGPNPWPILGNL QIGQNAHIS+TQF++ YGPLI
Sbjct: 9 LLLLLPLIFTILRHICSSS-KQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68
Query: 73 SLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWK 132
SLKLGAQRLVVASSPA A A+LKT DR LS RYI QMTPDR L++ SL+FSPECGD WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 133 NLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLI 192
NLRSIC+A LFTA+AIESQA LR KKM ++VEFL+SKQGSVV+IK+ VF +VFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 193 FSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDS 252
FS DL+ FVGDGFNGIKGSFK ++DLGL PNLADFYPLLR FDLQGLRKKAAIY N+VDS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248
Query: 253 LWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAM 312
W ILIKERR IH + SA+SDFLDVLI+SGF+D+QI+YLI E+LSAGTDTTTTTVEWAM
Sbjct: 249 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308
Query: 313 SEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAP 372
+E+LK++ I+N+VR+E KK IDES +S+L YLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
Query: 373 MDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLP 432
CE+MGYS+PKD+MIFVNVWGIGRDPSIWED +F PERFD G + D KG+DYR+LP
Sbjct: 369 EACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFD---GFDADLKGHDYRFLP 428
Query: 433 FGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALEL 492
FGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLGVTLQK E L+L
Sbjct: 429 FGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKL 488
Query: 493 IPKR 496
+ K+
Sbjct: 489 LSKK 488
BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match:
A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)
HSP 1 Score: 721 bits (1861), Expect = 4.81e-258
Identity = 362/485 (74.64%), Postives = 409/485 (84.33%), Query Frame = 0
Query: 12 SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPL 71
S LLL+P IF ILK I S S K+ P PPGP PWP+LGNL QIGQNAHIS+TQF++IYGPL
Sbjct: 7 SFLLLLPLIFAILKHISSSS-KQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 66
Query: 72 ISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHW 131
ISLKLGAQRLVVASSPA A A+LKT DR LS RYI QM PDRAL++ SLVFSPECGD W
Sbjct: 67 ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 126
Query: 132 KNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNL 191
KNLRSIC+A LFTA+AIESQA LR KKM E+VEFL+SKQGSVV+IK+FVF +VFNTLSN
Sbjct: 127 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNF 186
Query: 192 IFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVD 251
IFS DL+ FVGDGFNGI+G FK ++D L PNLADFYPLLR FDLQGLRKKAAIY N++D
Sbjct: 187 IFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQID 246
Query: 252 SLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWA 311
S W ILIKERR IH + +SDFLDVLIQSGF+D+QINYLI E+LSAGTDTTTTTVEWA
Sbjct: 247 SSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWA 306
Query: 312 MSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLA 371
M+E+LK++ I+N+VR+E KK IDES +S+L YLW CVKETLRLHPPAPFLLPR A
Sbjct: 307 MAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 366
Query: 372 PMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYL 431
P CE+MGYS+PK +MI VNVWGIGRDPSIWED Q+F PERFDV C + D KG+DYR+L
Sbjct: 367 PEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDV-C--DADLKGHDYRFL 426
Query: 432 PFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALE 491
PFGGGRR+CPGLPMA VQVPLILATL+HNFEW LPN ED S LDL GRLGVTLQK E L+
Sbjct: 427 PFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLK 486
Query: 492 LIPKR 496
L+PKR
Sbjct: 487 LLPKR 487
BLAST of CsGy5G005795 vs. TAIR 10
Match:
AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )
HSP 1 Score: 344.0 bits (881), Expect = 2.1e-94
Identity = 198/511 (38.75%), Postives = 299/511 (58.51%), Query Frame = 0
Query: 2 LIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISI 61
L P F +L + IF R +S S K +P PPGP PI+GN+ +G+N H S
Sbjct: 9 LFPLFCFVLSFFI-----IFFTTTRPRS-SRKVVPSPPGPPRLPIIGNIHLVGRNPHHSF 68
Query: 62 TQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRAL----YN 121
+ YGP++SLK G+ VV +SP A VL+T+D+ LS+R TP ++ ++
Sbjct: 69 ADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHD 128
Query: 122 QHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFL--ESKQGSVVK 181
+ S+V+ P W+ LR + LF+ + IE+ LRE K+KELV F+ S++ V
Sbjct: 129 KVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVD 188
Query: 182 IKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFD 241
I F + N +SN++FS+DL ++ + + + +M+ P+ A+F+P L D
Sbjct: 189 ISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLD 248
Query: 242 LQGLRKKAAIYMNEVDSLW-GILIKERREIHCQQGSASS----DFLDVLI------QSGF 301
LQG RK + ++ G + + E + ++ DF+DVL+ ++
Sbjct: 249 LQGNRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAEL 308
Query: 302 SDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT-KESPIDESNI 361
+ I +L+ +L AGTDT ++TVEWAM+E+L++ + K + EI V ++ ++ES+I
Sbjct: 309 NTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDI 368
Query: 362 SKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWE 421
S L YL + VKET RLHP AP L+PR A D EV+G+ +PKD+ +FVNVW IGRDP++WE
Sbjct: 369 SALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWE 428
Query: 422 DSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEW 481
+S F PERF G ++D +G DY PFG GRRICPGLP+A+ VPL+LA+LL++F+W
Sbjct: 429 NSSRFKPERF---LGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDW 488
Query: 482 SLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
LPNG LD++ G+TL K L +P
Sbjct: 489 KLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 505
BLAST of CsGy5G005795 vs. TAIR 10
Match:
AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 179/480 (37.29%), Postives = 286/480 (59.58%), Query Frame = 0
Query: 37 LPPGPNPWPILGNLPQI-GQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLK 96
LPPGPNPWP++GN+ Q+ G H S+T+ + +GP+++L++G+ VV SS A + K
Sbjct: 36 LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95
Query: 97 THDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILR 156
HD L+ R I + + + + + + G +W+ LR +C F + +++ + +R
Sbjct: 96 KHDAALAGRKIYEAM--KGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155
Query: 157 EKKMKELVEFLESKQGSVVKIKD---FVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSF 216
+ + +++ F+E + K D + F FN + NL+FS DLLD D G + +
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFY 215
Query: 217 K--KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGS 276
K+M+ PN+ADF+PLLR D QG+R+K ++ + + G I+ER E+ ++ S
Sbjct: 216 HTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKS 275
Query: 277 --ASSDFLDVLIQ-----------SGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILK 336
+ D+LDVL++ S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLH 335
Query: 337 DKGILNKVRQEIKKVTKES--PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDC 396
+ L K++ E++ K S + E ++ L YL + + ETLRLHPP PFL+P A C
Sbjct: 336 NPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTC 395
Query: 397 EVMG-YSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFG 456
+ Y+IPK++ + VNVW IGRDP W D F PERF + + DFKG DY +LPFG
Sbjct: 396 HIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFG 455
Query: 457 GGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
GRR+CP LP+A +PL + +++ +F+W+L NG + ++D+ R+G+TL+K LE IP
Sbjct: 456 SGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510
BLAST of CsGy5G005795 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 334.0 bits (855), Expect = 2.1e-91
Identity = 191/497 (38.43%), Postives = 289/497 (58.15%), Query Frame = 0
Query: 13 LLLLIPPIFII--LKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGP 72
L I F+I R + S + LPPGP PI+GN+ Q+G+N H S A IYGP
Sbjct: 12 LFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGP 71
Query: 73 LISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDH 132
++SLK G VV +SP A VL+THD+ LS R + + S+++ P
Sbjct: 72 IMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSAR 131
Query: 133 WKNLRSICKANLFTAKAIESQAILREKKMKELVEFL--ESKQGSVVKIKDFVFTSVFNTL 192
W+ LR + +F+ + E+ LR KK++ELV F+ S++ V I +T+V N +
Sbjct: 132 WRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNII 191
Query: 193 SNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMN 252
SN++FS+DL + N + + M+ P+ A+++P + DLQG RK
Sbjct: 192 SNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTE 251
Query: 253 EVDSLW-GIL---IKERREIHCQQGSASSDFLDVLI------QSGFSDQQINYLINELLS 312
+ ++ G + I E+ + + ++ DFLD L+ ++ + I +L+ ++ +
Sbjct: 252 RLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFT 311
Query: 313 AGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT-KESPIDESNISKLQYLWSCVKETL 372
AGTDT+++T+EWAM+E+L++ + K + E+ +V + S + ES+IS L YL + VKET
Sbjct: 312 AGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETF 371
Query: 373 RLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGC 432
RLHP AP L+PR A D EV+G+ +PKD+ + VNVW IGRDPS+WE+ F PERF
Sbjct: 372 RLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF---M 431
Query: 433 GNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLN 492
G ++D KG DY PFGGGRRICPGLP+A+ V L+LA+LL++F+W LPNG LD++
Sbjct: 432 GKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMD 491
Query: 493 GRLGVTLQKYEALELIP 495
G+TL + L IP
Sbjct: 492 ETFGITLHRTNTLYAIP 504
BLAST of CsGy5G005795 vs. TAIR 10
Match:
AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )
HSP 1 Score: 330.9 bits (847), Expect = 1.8e-90
Identity = 185/470 (39.36%), Postives = 269/470 (57.23%), Query Frame = 0
Query: 38 PPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTH 97
PPGP PI+GN+ +G+N H S T + YGP++SLKLG VV +S VLKTH
Sbjct: 39 PPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTH 98
Query: 98 DRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREK 157
D+ LS RYI + T +++ S+ + ++ LR + LF+ + I++ LR K
Sbjct: 99 DQILSGRYISEATKSNN-HHEFSVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMK 158
Query: 158 KMKELVEFLES--KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKM 217
K++ELV FL ++ V I F + N +SN++FS++L + + +
Sbjct: 159 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGY 218
Query: 218 MDLGLTPNLADFYPLLRRFDLQG----LRKKAAIYMNEVDSLWGILIKERREIHCQQGSA 277
+ P+LA+F+P +R DLQG +R+ + + + I E+ ++ +
Sbjct: 219 QESIGNPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVS 278
Query: 278 SSDFLDVLIQSGFSDQ------QINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKV 337
S DFLDVLI D+ +I +L+ ++ AGTDT ++TVEWAM+E+L + + KV
Sbjct: 279 SKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKV 338
Query: 338 RQEIKKVT-KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPK 397
+ EI V + ES+ISKL YL + VKET RLHP APFLL R A + E++G+++ K
Sbjct: 339 QDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLK 398
Query: 398 DSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLP 457
DS + VNVW IGRDP +WE+ F PERF G +D KG DY PFG GRRICPGLP
Sbjct: 399 DSQVLVNVWAIGRDPLVWENPTHFEPERF---LGKEIDVKGTDYELTPFGAGRRICPGLP 458
Query: 458 MAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
+A+ V L+LA+LL+ FEW LPNG LD+ G+T+ K L +P
Sbjct: 459 LAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLTVHKTNPLLAVP 504
BLAST of CsGy5G005795 vs. TAIR 10
Match:
AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 330.9 bits (847), Expect = 1.8e-90
Identity = 178/494 (36.03%), Postives = 287/494 (58.10%), Query Frame = 0
Query: 37 LPPGPNPWPILGNLPQI-GQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLK 96
LPPGPNPWP++GN+ Q+ G H S+T+ + +GP+++L++G+ VV SS A + K
Sbjct: 36 LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95
Query: 97 THDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILR 156
HD L+ R I + + + + + + G +W+ LR +C F + +++ + +R
Sbjct: 96 KHDAALAGRKIYEAM--KGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155
Query: 157 EKKMKELVEFLE--SKQG---------------SVVKIKDFVFTSVFNTLSNLIFSMDLL 216
+ + +++ F+E + G + + + F FN + NL+FS DLL
Sbjct: 156 SRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTKTIDVGRYFFLMAFNLIGNLMFSRDLL 215
Query: 217 DFVGDGFNGIKGSFK--KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGI 276
D D G + + K+M+ PN+ADF+PLLR D QG+R+K ++ + + G
Sbjct: 216 D--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGE 275
Query: 277 LIKERREIHCQQGS--ASSDFLDVLIQ-----------SGFSDQQINYLINELLSAGTDT 336
I+ER E+ ++ S + D+LDVL++ S FS + IN ++ E+ +AGTDT
Sbjct: 276 FIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDT 335
Query: 337 TTTTVEWAMSEILKDKGILNKVRQEIKKVTKES--PIDESNISKLQYLWSCVKETLRLHP 396
TT+T+EWA++E+L + L K++ E++ K S + E ++ L YL + + ETLRLHP
Sbjct: 336 TTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHP 395
Query: 397 PAPFLLPRLAPMDCEVMG-YSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 456
P PFL+P A C + Y+IPK++ + VNVW IGRDP W D F PERF + +
Sbjct: 396 PLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERF-ISDPDA 455
Query: 457 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 495
DFKG DY +LPFG GRR+CP LP+A +PL + +++ +F+W+L NG + ++D+ R+
Sbjct: 456 RDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERI 515
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
O64899 | 5.8e-118 | 46.00 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... | [more] |
Q9FXW4 | 2.2e-117 | 45.78 | Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... | [more] |
Q9SP06 | 2.9e-117 | 45.67 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... | [more] |
O64900 | 4.9e-117 | 45.67 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... | [more] |
A8CDR5 | 3.2e-108 | 42.86 | Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_031741123.1 | 0.0 | 100.00 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus] | [more] |
KAE8647950.1 | 0.0 | 97.99 | hypothetical protein Csa_000545 [Cucumis sativus] | [more] |
XP_008467223.1 | 0.0 | 90.62 | PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... | [more] |
KAA0055170.1 | 0.0 | 90.42 | putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... | [more] |
XP_031741076.1 | 0.0 | 90.74 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus] | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CT14 | 0.0 | 90.62 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... | [more] |
A0A5A7UIU3 | 0.0 | 90.42 | Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... | [more] |
A0A6J1ELA6 | 6.75e-263 | 76.60 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
A0A6J1I4V7 | 3.19e-261 | 75.41 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... | [more] |
A0A6J1ER52 | 4.81e-258 | 74.64 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
Match Name | E-value | Identity | Description | |
AT2G45570.1 | 2.1e-94 | 38.75 | cytochrome P450, family 76, subfamily C, polypeptide 2 | [more] |
AT3G52970.1 | 2.5e-92 | 37.29 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
AT2G45550.1 | 2.1e-91 | 38.43 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |
AT1G33720.1 | 1.8e-90 | 39.36 | cytochrome P450, family 76, subfamily C, polypeptide 6 | [more] |
AT3G52970.2 | 1.8e-90 | 36.03 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |