CsGy5G005795 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G005795
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionCytochrome p450 76c2
LocationGy14Chr5: 3816376 .. 3818549 (+)
RNA-Seq ExpressionCsGy5G005795
SyntenyCsGy5G005795
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCGAAGTAAAACTTCCACAATAATTCTAAGCCATATCTTAACTCCAACTCCATATTTCAAAGACATGTTGATTCCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTCATACCTCCAATTTTTATTATTCTCAAACGCATCAAATCTCCTTCTTTAAAAAAACTCCCACTTCCTCCTGGTCCAAATCCATGGCCAATCTTAGGAAATCTCCCTCAAATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCAAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTGGTCGTGGCTTCTTCTCCTGCCACTGCCACTGCAGTTCTCAAGACGCATGATCGCTTTCTCTCCAATCGATACATCTTGCAAATGACCCCTGATAGAGCTCTCTACAATCAACACTCTCTTGTTTTCTCCCCTGAATGTGGGGATCATTGGAAGAATTTGAGGTCAATTTGTAAAGCGAACTTGTTTACAGCTAAAGCAATTGAATCGCAAGCTATTTTGAGAGAGAAGAAGATGAAGGAATTAGTGGAATTTTTGGAATCAAAACAGGGATCTGTCGTGAAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTTTCGATGGATTTGCTTGATTTTGTTGGCGATGGGTTTAACGGGATTAAAGGGTCTTTCAAGAAAATGATGGATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTGATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTCAACAAGGTTCAGCTTCAAGTGATTTCTTGGATGTTTTGATTCAAAGTGGATTCAGTGATCAACAAATCAATTACTTGATCAATGTAAGTACAAAATCACACTCTTTTAACTACTTTAAATAACACGAGAGTTCGATCATTTTATCTATCCAAATGTGTTTCGCGGACATGTCTCTATAAGTTCAATATTCTTTTATTTCTTTTAAGTAAAGATTGTTTAAACTTGAGGTTTTTTTCTTCATAACGCAATATGTGATGTGTTAATGTCGAATGAGTTAAATTTTGAAAACAATGAAAACATACTTTCTTATGTCAAATACTACTTAAATTGTGTTTGGTATTTTCTCCGTCCCTCTCACTTGTCCAATCTCCTTCCTTCCTCTACCACTTTCACTTGTGCTTATCGCTACTGTTTTACTTGTCTTTATAATATATATTTCAGTTATTACTCTTCTTATTTCCAAGTAAACTCAATTTATGGTGCATATACTATAACGACCGTGAGATCTCTTCATTTTAAAACAGATAGATTTATAATCAAACATATCTAATGCATTATTAATTATTACTACTTTTGCAGGAATTGCTATCTGCAGGCACAGACACAACTACAACAACAGTGGAATGGGCAATGTCGGAGATTCTAAAAGACAAAGGCATACTAAACAAAGTAAGACAGGAAATAAAGAAGGTAACAAAGGAAAGCCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACATCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAATGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTGAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTACGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTACTTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTCACATTGCAAAAGTATGAAGCTCTTGAACTTATTCCTAAAAGGAACCCTATTTGAAATGATTGTCAACATTCATTATTTATGTATAATAATAAAGCTTTATTACTACATGTTTGTCATATACTATTTATATAAATCCAAGCTTGTGCGTACAAATTATACATATTCC

mRNA sequence

GATCGAAGTAAAACTTCCACAATAATTCTAAGCCATATCTTAACTCCAACTCCATATTTCAAAGACATGTTGATTCCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTCATACCTCCAATTTTTATTATTCTCAAACGCATCAAATCTCCTTCTTTAAAAAAACTCCCACTTCCTCCTGGTCCAAATCCATGGCCAATCTTAGGAAATCTCCCTCAAATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCAAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTGGTCGTGGCTTCTTCTCCTGCCACTGCCACTGCAGTTCTCAAGACGCATGATCGCTTTCTCTCCAATCGATACATCTTGCAAATGACCCCTGATAGAGCTCTCTACAATCAACACTCTCTTGTTTTCTCCCCTGAATGTGGGGATCATTGGAAGAATTTGAGGTCAATTTGTAAAGCGAACTTGTTTACAGCTAAAGCAATTGAATCGCAAGCTATTTTGAGAGAGAAGAAGATGAAGGAATTAGTGGAATTTTTGGAATCAAAACAGGGATCTGTCGTGAAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTTTCGATGGATTTGCTTGATTTTGTTGGCGATGGGTTTAACGGGATTAAAGGGTCTTTCAAGAAAATGATGGATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTGATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTCAACAAGGTTCAGCTTCAAGTGATTTCTTGGATGTTTTGATTCAAAGTGGATTCAGTGATCAACAAATCAATTACTTGATCAATGAATTGCTATCTGCAGGCACAGACACAACTACAACAACAGTGGAATGGGCAATGTCGGAGATTCTAAAAGACAAAGGCATACTAAACAAAGTAAGACAGGAAATAAAGAAGGTAACAAAGGAAAGCCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACATCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAATGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTGAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTACGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTACTTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTCACATTGCAAAAGTATGAAGCTCTTGAACTTATTCCTAAAAGGAACCCTATTTGAAATGATTGTCAACATTCATTATTTATGTATAATAATAAAGCTTTATTACTACATGTTTGTCATATACTATTTATATAAATCCAAGCTTGTGCGTACAAATTATACATATTCC

Coding sequence (CDS)

ATGTTGATTCCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTCATACCTCCAATTTTTATTATTCTCAAACGCATCAAATCTCCTTCTTTAAAAAAACTCCCACTTCCTCCTGGTCCAAATCCATGGCCAATCTTAGGAAATCTCCCTCAAATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCAAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTGGTCGTGGCTTCTTCTCCTGCCACTGCCACTGCAGTTCTCAAGACGCATGATCGCTTTCTCTCCAATCGATACATCTTGCAAATGACCCCTGATAGAGCTCTCTACAATCAACACTCTCTTGTTTTCTCCCCTGAATGTGGGGATCATTGGAAGAATTTGAGGTCAATTTGTAAAGCGAACTTGTTTACAGCTAAAGCAATTGAATCGCAAGCTATTTTGAGAGAGAAGAAGATGAAGGAATTAGTGGAATTTTTGGAATCAAAACAGGGATCTGTCGTGAAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTTTCGATGGATTTGCTTGATTTTGTTGGCGATGGGTTTAACGGGATTAAAGGGTCTTTCAAGAAAATGATGGATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTGATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTCAACAAGGTTCAGCTTCAAGTGATTTCTTGGATGTTTTGATTCAAAGTGGATTCAGTGATCAACAAATCAATTACTTGATCAATGAATTGCTATCTGCAGGCACAGACACAACTACAACAACAGTGGAATGGGCAATGTCGGAGATTCTAAAAGACAAAGGCATACTAAACAAAGTAAGACAGGAAATAAAGAAGGTAACAAAGGAAAGCCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACATCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAATGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTGAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTACGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTACTTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTCACATTGCAAAAGTATGAAGCTCTTGAACTTATTCCTAAAAGGAACCCTATTTGA

Protein sequence

MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPKRNPI*
Homology
BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 5.8e-118
Identity = 213/463 (46.00%), Postives = 306/463 (66.09%), Query Frame = 0

Query: 37  LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
           LPPGP PWPI+GNL Q+G+  H    + A  YG + +LK+G + +VVAS+ + A+ +LKT
Sbjct: 28  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87

Query: 97  HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
           HDR LS RY+ Q    +  + ++S+V+S +C + WKNLR +C+  LFT K IESQA +RE
Sbjct: 88  HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 147

Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGD---GFNGIKGSFK 216
           KK +E+VE+L  KQG  VKI + +F ++ N   NLIFS ++ + +GD   G +  K    
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFE-LGDPNSGSSEFKEYLW 207

Query: 217 KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQG-SAS 276
           +M++LG + N AD++P+L +FDL G RK+ A  +  + ++WG +++ER+      G  + 
Sbjct: 208 RMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSK 267

Query: 277 SDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKV 336
           +DF+DV + SG +D QIN L+ EL  AGT+T+ +T+EWAM+E+ K+  I  K+R EI+ V
Sbjct: 268 NDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTV 327

Query: 337 TKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNV 396
             E  + ES+   L YL + VKETLRLHPP P LLPR A   C ++ Y+IPKD  I VN 
Sbjct: 328 VGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNA 387

Query: 397 WGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPL 456
           WGIGRDP  W D  TF+PERF     ++VDF+G D+  +PFG GRRICPGLP+A   + L
Sbjct: 388 WGIGRDPKTWTDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIAL 447

Query: 457 ILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
           ++AT + N +W LPNG  +  L +  + G+TLQK   L ++PK
Sbjct: 448 LVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 484

BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.2e-117
Identity = 228/498 (45.78%), Postives = 323/498 (64.86%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           M + S +I+  S LL    +F IL+  +  +     LPPGP P PI+GNL Q+G   H  
Sbjct: 1   MEVLSIAIVSFSFLLF---LFFILRDSRPKN-----LPPGPRPSPIVGNLLQLGDKPHAE 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
             + A  YG L SLKLG+Q +VVASSPA A  +LKTHD+ LS RY+ Q    +  + ++S
Sbjct: 61  FAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           +V+S EC D+WK LR +C+  LFT K IESQ+ +RE K +E+V+FL  K+G VVKI + V
Sbjct: 121 IVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVG--DGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQG 240
           F ++ N   NLIFS D+ D      G   +K    K++D+G + N AD++P++ + DL G
Sbjct: 181 FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFG 240

Query: 241 LRKKAAIYMNEVDSLWGILIKERREIHCQQGSAS-SDFLDVLIQSGFSDQQINYLINELL 300
            R+  A  + ++  +WG+++KERR     +GS S +DF+DVL+ +G  DQ+IN L+ EL 
Sbjct: 241 QRRAVAEVLQQIYDVWGVMLKERRG---TKGSESKNDFVDVLLNAGLDDQKINALLMELF 300

Query: 301 SAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETL 360
            AGT+T+ +T+EWA++E+ K   +++K+R E+  V  ++ + ES++  L YL + VKETL
Sbjct: 301 GAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETL 360

Query: 361 RLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGC 420
           RLHPP P LLPR A   C VM Y+IPK+  I VN W IGRDP  W+D   F PERF    
Sbjct: 361 RLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERF---L 420

Query: 421 GNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLN 480
            ++VD+KG D+  +PFGGGRRICPGLP+A     LI+ATL+ NFEWSLP G   S+L ++
Sbjct: 421 SSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMD 480

Query: 481 GRLGVTLQKYEALELIPK 496
            + G+TLQK   L ++ K
Sbjct: 481 EKFGLTLQKDPPLLIVLK 482

BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.9e-117
Identity = 211/462 (45.67%), Postives = 302/462 (65.37%), Query Frame = 0

Query: 37  LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
           LPPGP PWPI+GNL Q+G+  H    Q A  YG L SLKLG++ +VVAS+P  A+ +LKT
Sbjct: 23  LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKT 82

Query: 97  HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
           HDR LS RY+ Q    +  + ++S+V+S EC + WK LR +C+  LFT K IESQA +RE
Sbjct: 83  HDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRE 142

Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNG---IKGSFK 216
            K  E+VE+L+   G+ VKI + VF ++ N   NLIFS ++   +GD  +G   +K    
Sbjct: 143 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFK-LGDESSGSVEMKEHLW 202

Query: 217 KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASS 276
           +M++LG + N AD++P L +FDL G  K  A  +  + S+WG ++KER+       S  +
Sbjct: 203 RMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKN 262

Query: 277 DFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT 336
           DF+++L+ SG  DQQIN L+ E+  AGT+T+ +T+EWA+SE+ K+  +   +R E+  V 
Sbjct: 263 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVV 322

Query: 337 KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVW 396
            + P+ ES+I  + YL + VKETLRLHP  P LLPR A   C+V+ Y+IPK+  I VN W
Sbjct: 323 GKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAW 382

Query: 397 GIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLI 456
           GIGRDP  W D   F+PERF     +++DFKG D+  +PFG GRRICPG+P+A   + LI
Sbjct: 383 GIGRDPKRWTDPLKFSPERF---LNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLI 442

Query: 457 LATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
           +++L+ NF+W  P G D S+L +  + G+TLQK   L ++PK
Sbjct: 443 VSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPK 478

BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.9e-117
Identity = 211/462 (45.67%), Postives = 303/462 (65.58%), Query Frame = 0

Query: 37  LPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKT 96
           LPPGP PWPI+GNL Q+G+  H    + A  YG + +LK+G + +VVAS+ + A+ +LKT
Sbjct: 29  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 88

Query: 97  HDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILRE 156
           HDR LS RY+ Q    +  + ++S+V+S +C + WKNLR +C+  LFT K IESQA +RE
Sbjct: 89  HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 148

Query: 157 KKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLIFSMDL--LDFVGDGFNGIKGSFKK 216
           KK +E+VE+L  KQG  VKI + +F ++ N   NLIFS ++  L     G +  K    +
Sbjct: 149 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWR 208

Query: 217 MMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQG-SASS 276
           M++LG + N AD++P+L +FDL G RK+ A  +  + ++WG +++ER+      G  + +
Sbjct: 209 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKN 268

Query: 277 DFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT 336
           DF+DV + SG +D QIN L+ EL  AGT+T+ +T+EWAM+E+ K+  I  K+R E++ V 
Sbjct: 269 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVV 328

Query: 337 KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVW 396
            E  + ES+   L YL + VKETLRLHPP P LLPR A   C ++ Y+IPKD  I VN W
Sbjct: 329 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 388

Query: 397 GIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLI 456
           GIGRDP  W D  TF+PERF     ++VDF+G D+  +PFG GRRICPGLP+A   + L+
Sbjct: 389 GIGRDPKTWIDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALL 448

Query: 457 LATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIPK 496
           +AT + N +W LPNG  +  L +  + G+TLQK   L ++PK
Sbjct: 449 VATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 485

BLAST of CsGy5G005795 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 3.2e-108
Identity = 210/490 (42.86%), Postives = 313/490 (63.88%), Query Frame = 0

Query: 12  SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNA----HISITQFANI 71
           +L  LIP I + +  +K P  K   LPPGP+PWP++GNLP +  N     HI++   A  
Sbjct: 6   ALFSLIPVILVFILLLK-PKYKN--LPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 65

Query: 72  YGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPEC 131
           +GP++ L LG Q  V+AS+   A  +LKTHDR  S R+I +M+     + ++SLV+S +C
Sbjct: 66  HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS-DC 125

Query: 132 GDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNT 191
            D+WK LR I +  +F+ K +++Q+ +RE+K+ EL++FL SK+G VVKI  FVF ++ N 
Sbjct: 126 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 185

Query: 192 LSNLIFSMDLLDFVGDGFN--GIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAI 251
           L N++FS D+  + GD  +  GI+   ++M+ +G  PN+A+FYP L   DLQGL+KK   
Sbjct: 186 LGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDE 245

Query: 252 YMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTT 311
               V  +W   +KER+     +   S D LDVL+ + F+D QIN L  E    G++T++
Sbjct: 246 RFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSS 305

Query: 312 TTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPF 371
            T+EW ++E++K    + KVR+E+ +V   S I ES++ +L YL +C+KE +RLHP APF
Sbjct: 306 ATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPF 365

Query: 372 LLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKG 431
           LLPR A   CEVMGY+IPK+S + VN + IGRDP  W+D  TF PERF     ++VDF G
Sbjct: 366 LLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF---LESDVDFHG 425

Query: 432 YDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQ 491
             Y+++PFG GRR C G+P+A   +PLI+ +L+HN+++ LP G     L +N  L +TL 
Sbjct: 426 AHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLA 483

Query: 492 KYEALELIPK 496
              +L ++PK
Sbjct: 486 IDPSLCVVPK 483

BLAST of CsGy5G005795 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 996 bits (2574), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
           KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH 360
           TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH
Sbjct: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLH 360

Query: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 420
           PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 420

Query: 421 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 480
           VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL
Sbjct: 421 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 480

Query: 481 GVTLQKYEALELIPKRNPI 499
           GVTLQKYEALELIPKRNPI
Sbjct: 481 GVTLQKYEALELIPKRNPI 499

BLAST of CsGy5G005795 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 487/497 (97.99%), Postives = 489/497 (98.39%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
           KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELL A 
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLFAA 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-IDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNK R+EIKKV KESP IDESNISKLQYLWSCVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 360

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPP PFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN
Sbjct: 361 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420

Query: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480
           NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480

Query: 481 LGVTLQKYEALELIPKR 496
           LGVTLQKYE L+LIPKR
Sbjct: 481 LGVTLQKYETLKLIPKR 497

BLAST of CsGy5G005795 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/501 (90.62%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLL+P IFIILK IKSPS  KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDLLDFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
            KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
            NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 481 RLGVTLQKYEALELIPKRNPI 499
           RLGVTLQKYE L+LIPKR  I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005795 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLL+P IFIILK IKSPS  KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDL+DFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
            KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
            NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 481 RLGVTLQKYEALELIPKRNPI 499
           RLGVTLQKYE L+LIPKR  I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005795 vs. NCBI nr
Match: XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 451/497 (90.74%), Postives = 466/497 (93.76%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLL+PPIFIILKRIKSPS KKLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLIS KLGAQRLVVASSPA ATAVLKT D  LS RYI Q+TPDRAL++QHS
Sbjct: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFS ECGD WK LRSICK NLFTAKAIESQAILREKKMKELVEF+ESKQGSVV+IKDFV
Sbjct: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTS FN LSNLIFSMDLLDFVGDGFNGIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
           +K  IY NEV+S+WGILIKERREI C++GSAS+DFLDVLIQSGFSDQQI+ LI ELL A 
Sbjct: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-IDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNK R+EIKKV KESP IDESNISKLQYLWSCVKETLRL
Sbjct: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPP PFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN
Sbjct: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 430

Query: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480
           NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490

Query: 481 LGVTLQKYEALELIPKR 496
           LGVTLQKYE L+LIPKR
Sbjct: 491 LGVTLQKYETLKLIPKR 507

BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 454/501 (90.62%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLL+P IFIILK IKSPS  KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDLLDFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
            KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
            NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 481 RLGVTLQKYEALELIPKRNPI 499
           RLGVTLQKYE L+LIPKR  I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60
           MLIPSFSILLPSLLLL+P IFIILK IKSPS  KLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPS-SKLPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120
           ITQFANIYGPLISLKLGAQRL+VASSPA ATAVLKT DR LS RYI QMTPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180
           LVFSPECGD WKNLRS+CK NLFTAKAIESQAILREKKMKELV+FLESKQGSVV++KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240
           FTSVFNTLSNLIFSMDL+DFVGDGF GIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL+
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300
            KA IY NEVDSLWGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQIN LI ELLSAG
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKES-PIDESNISKLQYLWSCVKETLRL 360
           TDTTTTTVEWAMSEILKDK ILNKVR+E+KKVTKE+ PIDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420
           HPPAPFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTFNP+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 421 -NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 480
            NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 481 RLGVTLQKYEALELIPKRNPI 499
           RLGVTLQKYE L+LIPKR  I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 733 bits (1893), Expect = 6.75e-263
Identity = 370/483 (76.60%), Postives = 414/483 (85.71%), Query Frame = 0

Query: 13  LLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLI 72
           LLLL+P IFIIL+ I S S K+ PLPPGP PWPILGNL QIGQNAHIS+TQF++IYGPLI
Sbjct: 9   LLLLLPLIFIILRHISSSS-KQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 73  SLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWK 132
           SLKLGAQRLVVASSPA A A+LKT DR LS RYI QMTPDR L++  SL+FSPECGD WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 133 NLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLI 192
           NLRSIC+A LFTA+AIESQA LR KKM E+VEFL+SKQGSVV+IK+ VF +VFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 193 FSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDS 252
           FS DL+ FVGDGFNGIKG FK M+DLGL PNLADFYPLLR FDLQGLRKKAAIY N+VDS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248

Query: 253 LWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAM 312
            W ILIKERR IH Q  SA+SDFLDVLIQSGF+D+QI+YLI E+LSAGTDTTTTTVEWAM
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308

Query: 313 SEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAP 372
           +E+LK++ I+N+VR+E KK      IDES +S+L YLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 373 MDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLP 432
             CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+F PERFD GC  + D KG+DYR+LP
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--DADLKGHDYRFLP 428

Query: 433 FGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALEL 492
           FGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLGVTLQK E L+L
Sbjct: 429 FGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKL 487

Query: 493 IPK 495
           + K
Sbjct: 489 LSK 487

BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 729 bits (1882), Expect = 3.19e-261
Identity = 365/484 (75.41%), Postives = 414/484 (85.54%), Query Frame = 0

Query: 13  LLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPLI 72
           LLLL+P IF IL+ I S S K+ PLPPGPNPWPILGNL QIGQNAHIS+TQF++ YGPLI
Sbjct: 9   LLLLLPLIFTILRHICSSS-KQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 73  SLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWK 132
           SLKLGAQRLVVASSPA A A+LKT DR LS RYI QMTPDR L++  SL+FSPECGD WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 133 NLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNLI 192
           NLRSIC+A LFTA+AIESQA LR KKM ++VEFL+SKQGSVV+IK+ VF +VFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 193 FSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDS 252
           FS DL+ FVGDGFNGIKGSFK ++DLGL PNLADFYPLLR FDLQGLRKKAAIY N+VDS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248

Query: 253 LWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWAM 312
            W ILIKERR IH +  SA+SDFLDVLI+SGF+D+QI+YLI E+LSAGTDTTTTTVEWAM
Sbjct: 249 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308

Query: 313 SEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAP 372
           +E+LK++ I+N+VR+E KK      IDES +S+L YLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 373 MDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLP 432
             CE+MGYS+PKD+MIFVNVWGIGRDPSIWED  +F PERFD   G + D KG+DYR+LP
Sbjct: 369 EACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFD---GFDADLKGHDYRFLP 428

Query: 433 FGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALEL 492
           FGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLGVTLQK E L+L
Sbjct: 429 FGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKL 488

Query: 493 IPKR 496
           + K+
Sbjct: 489 LSKK 488

BLAST of CsGy5G005795 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 4.81e-258
Identity = 362/485 (74.64%), Postives = 409/485 (84.33%), Query Frame = 0

Query: 12  SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPL 71
           S LLL+P IF ILK I S S K+ P PPGP PWP+LGNL QIGQNAHIS+TQF++IYGPL
Sbjct: 7   SFLLLLPLIFAILKHISSSS-KQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 66

Query: 72  ISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHW 131
           ISLKLGAQRLVVASSPA A A+LKT DR LS RYI QM PDRAL++  SLVFSPECGD W
Sbjct: 67  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 126

Query: 132 KNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNL 191
           KNLRSIC+A LFTA+AIESQA LR KKM E+VEFL+SKQGSVV+IK+FVF +VFNTLSN 
Sbjct: 127 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNF 186

Query: 192 IFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVD 251
           IFS DL+ FVGDGFNGI+G FK ++D  L PNLADFYPLLR FDLQGLRKKAAIY N++D
Sbjct: 187 IFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQID 246

Query: 252 SLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTDTTTTTVEWA 311
           S W ILIKERR IH +    +SDFLDVLIQSGF+D+QINYLI E+LSAGTDTTTTTVEWA
Sbjct: 247 SSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWA 306

Query: 312 MSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLA 371
           M+E+LK++ I+N+VR+E KK      IDES +S+L YLW CVKETLRLHPPAPFLLPR A
Sbjct: 307 MAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 366

Query: 372 PMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYL 431
           P  CE+MGYS+PK +MI VNVWGIGRDPSIWED Q+F PERFDV C  + D KG+DYR+L
Sbjct: 367 PEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDV-C--DADLKGHDYRFL 426

Query: 432 PFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALE 491
           PFGGGRR+CPGLPMA VQVPLILATL+HNFEW LPN ED S LDL GRLGVTLQK E L+
Sbjct: 427 PFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLK 486

Query: 492 LIPKR 496
           L+PKR
Sbjct: 487 LLPKR 487

BLAST of CsGy5G005795 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-94
Identity = 198/511 (38.75%), Postives = 299/511 (58.51%), Query Frame = 0

Query: 2   LIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISI 61
           L P F  +L   +     IF    R +S S K +P PPGP   PI+GN+  +G+N H S 
Sbjct: 9   LFPLFCFVLSFFI-----IFFTTTRPRS-SRKVVPSPPGPPRLPIIGNIHLVGRNPHHSF 68

Query: 62  TQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRAL----YN 121
              +  YGP++SLK G+   VV +SP  A  VL+T+D+ LS+R     TP  ++    ++
Sbjct: 69  ADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHD 128

Query: 122 QHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFL--ESKQGSVVK 181
           + S+V+ P     W+ LR +    LF+ + IE+   LRE K+KELV F+   S++   V 
Sbjct: 129 KVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVD 188

Query: 182 IKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFD 241
           I    F +  N +SN++FS+DL ++  +     + +   +M+    P+ A+F+P L   D
Sbjct: 189 ISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLD 248

Query: 242 LQGLRKKAAIYMNEVDSLW-GILIKERREIHCQQGSASS----DFLDVLI------QSGF 301
           LQG RK        +  ++ G +  +  E   +  ++      DF+DVL+      ++  
Sbjct: 249 LQGNRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAEL 308

Query: 302 SDQQINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT-KESPIDESNI 361
           +   I +L+ +L  AGTDT ++TVEWAM+E+L++   + K + EI  V  ++  ++ES+I
Sbjct: 309 NTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDI 368

Query: 362 SKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWE 421
           S L YL + VKET RLHP AP L+PR A  D EV+G+ +PKD+ +FVNVW IGRDP++WE
Sbjct: 369 SALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWE 428

Query: 422 DSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEW 481
           +S  F PERF    G ++D +G DY   PFG GRRICPGLP+A+  VPL+LA+LL++F+W
Sbjct: 429 NSSRFKPERF---LGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDW 488

Query: 482 SLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
            LPNG     LD++   G+TL K   L  +P
Sbjct: 489 KLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 505

BLAST of CsGy5G005795 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 179/480 (37.29%), Postives = 286/480 (59.58%), Query Frame = 0

Query: 37  LPPGPNPWPILGNLPQI-GQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLK 96
           LPPGPNPWP++GN+ Q+ G   H S+T+ +  +GP+++L++G+   VV SS   A  + K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 97  THDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILR 156
            HD  L+ R I +    +   +    + + + G +W+ LR +C    F  + +++ + +R
Sbjct: 96  KHDAALAGRKIYEAM--KGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 157 EKKMKELVEFLESKQGSVVKIKD---FVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSF 216
            + + +++ F+E    +  K  D   + F   FN + NL+FS DLLD   D   G +  +
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFY 215

Query: 217 K--KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGS 276
              K+M+    PN+ADF+PLLR  D QG+R+K   ++ +   + G  I+ER E+  ++ S
Sbjct: 216 HTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKS 275

Query: 277 --ASSDFLDVLIQ-----------SGFSDQQINYLINELLSAGTDTTTTTVEWAMSEILK 336
              + D+LDVL++           S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L 
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLH 335

Query: 337 DKGILNKVRQEIKKVTKES--PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDC 396
           +   L K++ E++   K S   + E ++  L YL + + ETLRLHPP PFL+P  A   C
Sbjct: 336 NPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTC 395

Query: 397 EVMG-YSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFG 456
            +   Y+IPK++ + VNVW IGRDP  W D   F PERF +   +  DFKG DY +LPFG
Sbjct: 396 HIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFG 455

Query: 457 GGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
            GRR+CP LP+A   +PL + +++ +F+W+L NG +  ++D+  R+G+TL+K   LE IP
Sbjct: 456 SGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510

BLAST of CsGy5G005795 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 334.0 bits (855), Expect = 2.1e-91
Identity = 191/497 (38.43%), Postives = 289/497 (58.15%), Query Frame = 0

Query: 13  LLLLIPPIFII--LKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGP 72
           L   I   F+I    R +  S +   LPPGP   PI+GN+ Q+G+N H S    A IYGP
Sbjct: 12  LFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGP 71

Query: 73  LISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDH 132
           ++SLK G    VV +SP  A  VL+THD+ LS R           + + S+++ P     
Sbjct: 72  IMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSAR 131

Query: 133 WKNLRSICKANLFTAKAIESQAILREKKMKELVEFL--ESKQGSVVKIKDFVFTSVFNTL 192
           W+ LR +    +F+ +  E+   LR KK++ELV F+   S++   V I    +T+V N +
Sbjct: 132 WRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNII 191

Query: 193 SNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMN 252
           SN++FS+DL  +     N  + +    M+    P+ A+++P +   DLQG RK       
Sbjct: 192 SNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTE 251

Query: 253 EVDSLW-GIL---IKERREIHCQQGSASSDFLDVLI------QSGFSDQQINYLINELLS 312
            +  ++ G +   I E+   +  +  ++ DFLD L+      ++   +  I +L+ ++ +
Sbjct: 252 RLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFT 311

Query: 313 AGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVT-KESPIDESNISKLQYLWSCVKETL 372
           AGTDT+++T+EWAM+E+L++   + K + E+ +V  + S + ES+IS L YL + VKET 
Sbjct: 312 AGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETF 371

Query: 373 RLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGC 432
           RLHP AP L+PR A  D EV+G+ +PKD+ + VNVW IGRDPS+WE+   F PERF    
Sbjct: 372 RLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF---M 431

Query: 433 GNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLN 492
           G ++D KG DY   PFGGGRRICPGLP+A+  V L+LA+LL++F+W LPNG     LD++
Sbjct: 432 GKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMD 491

Query: 493 GRLGVTLQKYEALELIP 495
              G+TL +   L  IP
Sbjct: 492 ETFGITLHRTNTLYAIP 504

BLAST of CsGy5G005795 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 330.9 bits (847), Expect = 1.8e-90
Identity = 185/470 (39.36%), Postives = 269/470 (57.23%), Query Frame = 0

Query: 38  PPGPNPWPILGNLPQIGQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTH 97
           PPGP   PI+GN+  +G+N H S T  +  YGP++SLKLG    VV +S      VLKTH
Sbjct: 39  PPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTH 98

Query: 98  DRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREK 157
           D+ LS RYI + T     +++ S+ +       ++ LR +    LF+ + I++   LR K
Sbjct: 99  DQILSGRYISEATKSNN-HHEFSVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMK 158

Query: 158 KMKELVEFLES--KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKM 217
           K++ELV FL    ++   V I    F +  N +SN++FS++L  +     +  +      
Sbjct: 159 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGY 218

Query: 218 MDLGLTPNLADFYPLLRRFDLQG----LRKKAAIYMNEVDSLWGILIKERREIHCQQGSA 277
            +    P+LA+F+P +R  DLQG    +R+ +   +      +   I E+     ++  +
Sbjct: 219 QESIGNPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVS 278

Query: 278 SSDFLDVLIQSGFSDQ------QINYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKV 337
           S DFLDVLI     D+      +I +L+ ++  AGTDT ++TVEWAM+E+L +   + KV
Sbjct: 279 SKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKV 338

Query: 338 RQEIKKVT-KESPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPK 397
           + EI  V  +     ES+ISKL YL + VKET RLHP APFLL R A  + E++G+++ K
Sbjct: 339 QDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLK 398

Query: 398 DSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLP 457
           DS + VNVW IGRDP +WE+   F PERF    G  +D KG DY   PFG GRRICPGLP
Sbjct: 399 DSQVLVNVWAIGRDPLVWENPTHFEPERF---LGKEIDVKGTDYELTPFGAGRRICPGLP 458

Query: 458 MAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYEALELIP 495
           +A+  V L+LA+LL+ FEW LPNG     LD+    G+T+ K   L  +P
Sbjct: 459 LAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLTVHKTNPLLAVP 504

BLAST of CsGy5G005795 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 330.9 bits (847), Expect = 1.8e-90
Identity = 178/494 (36.03%), Postives = 287/494 (58.10%), Query Frame = 0

Query: 37  LPPGPNPWPILGNLPQI-GQNAHISITQFANIYGPLISLKLGAQRLVVASSPATATAVLK 96
           LPPGPNPWP++GN+ Q+ G   H S+T+ +  +GP+++L++G+   VV SS   A  + K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 97  THDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILR 156
            HD  L+ R I +    +   +    + + + G +W+ LR +C    F  + +++ + +R
Sbjct: 96  KHDAALAGRKIYEAM--KGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 157 EKKMKELVEFLE--SKQG---------------SVVKIKDFVFTSVFNTLSNLIFSMDLL 216
            + + +++ F+E   + G                 + +  + F   FN + NL+FS DLL
Sbjct: 156 SRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTKTIDVGRYFFLMAFNLIGNLMFSRDLL 215

Query: 217 DFVGDGFNGIKGSFK--KMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVDSLWGI 276
           D   D   G +  +   K+M+    PN+ADF+PLLR  D QG+R+K   ++ +   + G 
Sbjct: 216 D--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGE 275

Query: 277 LIKERREIHCQQGS--ASSDFLDVLIQ-----------SGFSDQQINYLINELLSAGTDT 336
            I+ER E+  ++ S   + D+LDVL++           S FS + IN ++ E+ +AGTDT
Sbjct: 276 FIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDT 335

Query: 337 TTTTVEWAMSEILKDKGILNKVRQEIKKVTKES--PIDESNISKLQYLWSCVKETLRLHP 396
           TT+T+EWA++E+L +   L K++ E++   K S   + E ++  L YL + + ETLRLHP
Sbjct: 336 TTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHP 395

Query: 397 PAPFLLPRLAPMDCEVMG-YSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNN 456
           P PFL+P  A   C +   Y+IPK++ + VNVW IGRDP  W D   F PERF +   + 
Sbjct: 396 PLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERF-ISDPDA 455

Query: 457 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRL 495
            DFKG DY +LPFG GRR+CP LP+A   +PL + +++ +F+W+L NG +  ++D+  R+
Sbjct: 456 RDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERI 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O648995.8e-11846.00(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
Q9FXW42.2e-11745.78Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
Q9SP062.9e-11745.67(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
O649004.9e-11745.67(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
A8CDR53.2e-10842.86Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_031741123.10.0100.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus][more]
KAE8647950.10.097.99hypothetical protein Csa_000545 [Cucumis sativus][more]
XP_008467223.10.090.62PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... [more]
KAA0055170.10.090.42putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... [more]
XP_031741076.10.090.74probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A1S3CT140.090.62probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
A0A5A7UIU30.090.42Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
A0A6J1ELA66.75e-26376.60probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1I4V73.19e-26175.41probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A6J1ER524.81e-25874.64probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
AT2G45570.12.1e-9438.75cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT3G52970.12.5e-9237.29cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT2G45550.12.1e-9138.43cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT1G33720.11.8e-9039.36cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT3G52970.21.8e-9036.03cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 308..334
score: 24.36
coord: 430..440
score: 55.82
coord: 440..463
score: 34.18
coord: 65..84
score: 32.79
coord: 288..305
score: 31.73
coord: 350..368
score: 32.67
coord: 391..415
score: 33.92
IPR001128Cytochrome P450PRINTSPR00385P450coord: 351..362
score: 40.07
coord: 299..316
score: 36.59
coord: 431..440
score: 60.95
coord: 440..451
score: 28.57
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..482
e-value: 9.9E-97
score: 324.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..497
e-value: 1.3E-112
score: 378.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..491
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 17..468
e-value: 1.8E-18
score: 64.5
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 11..495
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 11..495
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 433..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G005795.1CsGy5G005795.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen