CsGy3G027240 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G027240
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Gy14Chr3: 28359271 .. 28360474 (-)
RNA-Seq Expression	CsGy3G027240
Synteny	CsGy3G027240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCGAGATCCATGGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAATTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAATATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATCTACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCACCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTAAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAAAAATATTTCAAATTATAAATTTTATTTCAATAATTCTGAGACTAAAACATCAAAATCATTCCAATTTTTTTTCCTACTAATTCACAATCATATGTTATGGTATTATTTTTCTGATCAAAATATTTTTTTTAAAAAAATTCAATTCTACTTCAAA

mRNA sequence

ATGGCCTCCGAGATCCATGGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAATTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAATATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATCTACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCACCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTAAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAAAAATATTTCAAATTATAAATTTTATTTCAATAATTCTGAGACTAAAACATCAAAATCATTCCAATTTTTTTTCCTACTAATTCACAATCATATGTTATGGTATTATTTTTCTGATCAAAATATTTTTTTTAAAAAAATTCAATTCTACTTCAAA

Coding sequence (CDS)

ATGGCCTCCGAGATCCATGGCCTCCTTCAAACTCTCTTCTTAGTTTCATCATCTCTTTCTCTCTGTTTCTCAATCAGAAATTCAATCCCGAATGGCTTCCTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCATACCATCCATTTCCAAGGCCCCATCGGCTTTTTCATTGGTTGGCTCGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCCCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGACCACAAATCCCCCCCAAATCATTCCCTTATCCCCCCTTCCGCAAAATTAATATTCCTAATCCTCACAATCCTCGCCATCAATTTCAAAAATCACCTCCATCCAAACGCAATTCCCCTGTTCTACTGCCTCCTAATCTACTTCTTCCTCGAATTCCTCATCGGCACCACCGCCACCCTAGCCAAAACAGTTCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATTTCTCTGATTCGTTACAGGATTTTTGGGGACGACGATGGAATCTAATGACCTCTAGAATCCTTCGCCTCACCATTTACGATCCATGCCGGAATCTAACCATCGGCGTCATCGGGAGGAGACCGGCGTCAATGGTGGCGGTGATAGCGACATTTGTAGTCTCTGGTCTTATGCACGAATTGATATATTTCTATATGGGAAGAATGGCGCCGACTTGGGAGGTGACTTGCTTCTTTGTGGTACATGGAGTTTGTGTGGTGGCGGAGATGGCGGTGAGGTTAGGCGCCGGTGGGAGGTTCCGGGCGCCGCGAGTTGCAAGAATATGTTTAACGATAATGTTTGTGATGGGAACGGGAAGTTGGCTTTTTTTCCCGCAGTGTATTAGGGCTAAAATGGACGTTAGAATGCTTGAAGAGTACGCCGCAATAGGTGCGTTTTTAAAGACTATTACGCTCCATTTCATCCCACCCTTTAACCCTACTTCTTTTCTATTCCATTAA

Protein sequence

MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPPSAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLFH*

Homology

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BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.4e-65
Identity = 138/325 (42.46%), Postives = 189/325 (58.15%), Query Frame = 0


Query: 14  LVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFK 73
           LV  SL   F I   +P G  + +L  P F +F  +P   +++H  G   FFI WL +FK
Sbjct: 10  LVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWLANFK 69

Query: 74  LLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIP---------DHKSPPNHSLIPPSAKL 133
           LLLFA G+GPL S     SLP FLAV  LPI+I           H+      LI  + K 
Sbjct: 70  LLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI-YTIKA 129

Query: 134 IFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPY 193
           +F++L I A  +   L    +   Y + IYF LE ++  TA   + +  +EL P FN+PY
Sbjct: 130 VFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPY 189

Query: 194 FSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHEL 253
            + SLQDFWGRRWNLM + ILR T+Y+P   L   V+G   + ++A   TFVVSG+MHEL
Sbjct: 190 LATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSGIMHEL 249

Query: 254 IYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSW 313
           I+FYMGR+ P W++  FF+++G C   E+A++    GR+R P+     LT+ FVM T  W
Sbjct: 250 IFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALW 309

Query: 314 LFFPQCIRAKMDVRMLEEYAAIGAF 330
           LF P+  R  +  + L+EYAAIGAF
Sbjct: 310 LFLPEFNRCNIVEKALDEYAAIGAF 332

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BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.4e-57
Identity = 125/303 (41.25%), Postives = 171/303 (56.44%), Query Frame = 0


Query: 32  GFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFKLLLFAFGKGPLCSAATSS 91
           G L+ LL+LP   LF+++PL+  + H  GP   ++ WL +FKLLLFAF  GPL +   S 
Sbjct: 35  GALRLLLLLPVVLLFIFLPLRLSSFHLGGPTALYLVWLANFKLLLFAFHLGPLSN--PSL 94

Query: 92  SLPRFLAVGSLPIEIPDHKSPPN-----------HSLIPPSAKLIFLILTILAINFKNHL 151
           SL  F++   LPI+  D  S  +             ++   AKL+F    +    F+  L
Sbjct: 95  SLLHFISTTLLPIKFRDDPSNDHEKNKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDL 154

Query: 152 HPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPYFSDSLQDFWGRRWNLM 211
               I + YC   Y   E  +  +A +A+  LG++L P FNEPY + SLQDFWGRRWNLM
Sbjct: 155 PHFVISVLYCFHFYLGTEITLAASAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLM 214

Query: 212 TSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHELIYFYMGRMAPTWEVTC 271
            S IL LT Y P R +    +  R     A++  F VSGLMHE+ +FY+ R  P+WEVT 
Sbjct: 215 VSDILGLTTYQPVRRVLSRWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTG 274

Query: 272 FFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSWLFFPQCIRAKMDVRML 324
           FFV+HGVC   EM V+    G+ R  R     LT+ FVM TG WLF PQ +R  +D++ +
Sbjct: 275 FFVLHGVCTAVEMVVKKAVSGKVRLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTI 334

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BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.8e-56
Identity = 130/335 (38.81%), Postives = 183/335 (54.63%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+  L++       S+S C+     I +G  + L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++  +  P N   +P 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ--MPK 120

Query: 121 ---SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 180
              + KL F  + +    +K H+ P  + + Y L IY   E L+     L    L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVV 240
           P+FNEPY S SLQDFWGRRWNLM   ILR  +Y P R +    +    A  + V A+F+V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV-RLGAGGRFRAPRVARICLTIM 300
           SG++HELI+FY  R +PT EVT FFV+HGVC  AE A  R     R++  ++    LT+ 
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLK 332
           FV+ TG WLFFP   R+ M  R+ +E      F+K
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-51
Identity = 129/343 (37.61%), Postives = 179/343 (52.19%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  +   L++       SLS C+ I + +  G L+ L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSP---PNHSL 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I    SP   PN   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 IPPSAKLIFLILTILAINFKNHLHPNAIPLF-----YCLLIYFFLEFLIGTTATLAKTVL 180
            P    ++ + + +L +    + + + +P F     YCL IY  +E ++     +  T+L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVI 240
           G  + P FNEPY + SLQDFW RRWNLM S +LR T++ P +     ++    A    V+
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARIC 300
           A+F VSGLMHEL+YFYM R  PTWEVTCFFV+HG     E+AV+       R P  A   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 LTIM-FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTIT 335
           L ++ FV  TG WLF  Q +R  +  + + E   +    K  T
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFT 339

[Close]

BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-51
Identity = 124/335 (37.01%), Postives = 184/335 (54.93%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+  L++       S+S C+ I + I  G L+ L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF      PI+I  +K+P ++ +   
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI--NKNPSSNRIHNK 120

Query: 121 SAKLIFLILTILAINFKNHLHPN---------AIPLFYCLLIYFFLEFLIGTTATLAKTV 180
                 L   +L  +F  H++ N         A    + + +Y   E ++     L   +
Sbjct: 121 PMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAV 240
           LG E+ P FN+PY + SLQ+FW RRWNLM   +LR  ++ P +     ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 IATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFR---APRV 300
           +ATF+VSGLMHELIYFY+ R +PTWEVTCFF++HGV    E+A++     R R    PR 
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 ARICLTI-MFVMGTGSWLFFPQCIRAKMDVRMLEE 323
           A   L I +F++ T  WLF+PQ +R  +  R++ E
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CsGy3G027240 vs. NCBI nr
Match: XP_004149743.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 691 bits (1783), Expect = 1.08e-250
Identity = 346/349 (99.14%), Postives = 346/349 (99.14%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIH LLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLFH 349
           TGSWLFFPQCIRAKMDVRMLEEYAAIGAF KTITLHFIPPFNPTSFLFH
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 349

[Close]

BLAST of CsGy3G027240 vs. NCBI nr
Match: KAE8650923.1 (hypothetical protein Csa_001633 [Cucumis sativus])

HSP 1 Score: 644 bits (1662), Expect = 1.47e-232
Identity = 324/327 (99.08%), Postives = 324/327 (99.08%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIH LLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIG 327
           TGSWLFFPQCIRAKMDVRMLEEYAAI 
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIA 327

[Close]

BLAST of CsGy3G027240 vs. NCBI nr
Match: XP_008461836.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa] >TYJ97432.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 612 bits (1578), Expect = 1.69e-219
Identity = 309/348 (88.79%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH LLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLF 348
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAFLK IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CsGy3G027240 vs. NCBI nr
Match: XP_038903196.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 578 bits (1489), Expect = 8.48e-206
Identity = 292/348 (83.91%), Postives = 315/348 (90.52%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH LLQTLFLVSSSL  CF+IRNSIPNGFLKFLLILPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---L 120
           PIGFFIGWLGSFKLLLFAFGKGPL SAATSSSLPRFLAVGSLPIEIPDH+SPPNHS   L
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKL 120

Query: 121 IPP---SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           + P   SAKL FLILT++AINFKNH HPNA  LFYCLLIYFFLEFLIG+TA +AK VLGV
Sbjct: 121 VLPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLPYFNEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCR LTIGVIGR+PA+M+AV+AT
Sbjct: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           FVVSGLMHELIYFY+GR+APTWEVTCFF+VHGVC+V EMAV+L AGGRFR P+V  I LT
Sbjct: 241 FVVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFN 342
           I FVMGTG WLFFPQC+RAKMDVRM EEYAAIGAF+K+IT+  I PF 
Sbjct: 301 IGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPFK 348

[Close]

BLAST of CsGy3G027240 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 499 bits (1285), Expect = 9.22e-175
Identity = 253/348 (72.70%), Postives = 283/348 (81.32%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EI   LQTLFLVSSSL  CF+IRNSIPNGFLKFLLILPFFFLFLYIPLQF +IHFQG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
            IGFFIGWLGSFKLLLFAFGKGPLCSAA SSSL  FLAVGSLPIEIPDHK   NHS   P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 ------SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
                 S+KL  LILTI+AI FKN  HPNAI +F+C +IY  LE L+G  A LAK  LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLPYF+EPY SDSLQDFWGRRWNLMTSRILRL +YDPCR +TIG++GR+PA+M+A+ AT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSGLMHELIYFYMGR+APTWEVTCFF++HGVC+ AEMA++   GGRFR P V    LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFN 342
           I FVM T  WLFFPQ ++ K DVRM EEYAA+GAF+K++T   I P N
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLN 348

[Close]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL
Match: A0A0A0L9R8 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=3 SV=1)

HSP 1 Score: 691 bits (1783), Expect = 5.23e-251
Identity = 346/349 (99.14%), Postives = 346/349 (99.14%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MASEIH LLQTLFLVSSSLSLCFSIRNSIPNGF KFLLILPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF
Sbjct: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL
Sbjct: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLFH 349
           TGSWLFFPQCIRAKMDVRMLEEYAAIGAF KTITLHFIPPFNPTSFLFH
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFNPTSFLFH 349

[Close]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL
Match: A0A5D3BC71 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00050 PE=3 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 8.17e-220
Identity = 309/348 (88.79%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH LLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLF 348
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAFLK IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL
Match: A0A1S3CG36 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500343 PE=3 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 8.17e-220
Identity = 309/348 (88.79%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH LLQTLFLVSSSLS+CFSIRNSIPNGFLKFLL+LPFFFLFLYIPLQFHTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
           PIGFFIGWLGSFKLLLFAFG+GPLCSAATSSSLPRFLAVGSLPIEIPDHKSPP+HSL+PP
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYF 180
           SAKL FLILTILAINFKNH H NA+ LFYCLLIYFFLEFLIGTTA LAK VLGVELLPYF
Sbjct: 121 SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVELLPYF 180

Query: 181 NEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGL 240
           NEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCRNLT+G+IGRR ASMVAV+ATF VSGL
Sbjct: 181 NEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATFGVSGL 240

Query: 241 MHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMG 300
           MHELIYFYMGRMAPTWEV+CFFVVHGVCVVAE AV+LGAGGR+RAPR  RI LTI FVMG
Sbjct: 241 MHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTIGFVMG 300

Query: 301 TGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPPFNPTSFLF 348
           TGSWLFFPQCIRAKMDVRMLEEYAA+GAFLK IT+ FI   +  SFLF
Sbjct: 301 TGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFI---DSISFLF 345

[Close]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 7.31e-167
Identity = 241/346 (69.65%), Postives = 281/346 (81.21%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH  +QTLFL++SSL LCFSIRNSIPNGFLKFL +LPFFFLFLYIPLQF TIH +G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---- 120
            +GFFIGWLGSFKLLLFAFGKGPLC AATSSSL RFLAV  LPI+IP+HKSPPN S    
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 LIPPS--AKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           L+P S  AKL FLILT  AI FKN  +PN + +F+CLLIY  LE LIG    LA+ VLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLP+F+EPY SDSLQDFWGRRWNL++SRILR  ++DPCR L   V+G++PA+++AV+AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSG+MHELIYFYMGR+ PTWEV+ FF++HGVC+ AEMAV+   GGRFR PRV    LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPP 340
           I FVM T  WLFFPQ +R K DVRMLEEYAA+GAF+K++T   I P
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGP 346

[Close]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 478 bits (1229), Expect = 1.47e-166
Identity = 241/346 (69.65%), Postives = 281/346 (81.21%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           MA+EIH  +QTLFL+SSSL LCFSIRNSIPNGFLKF+ +LPFFFLFLYIPLQF TIH +G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHS---- 120
            +GFFIGWLGSFKLLLFAFGKGPLCSAATSSSL RFLAV SLPI+IP+HK PPN S    
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 LIPPS--AKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
           L+P S  AKL FLILT  AI FKN  +PN + +F+CLLIY  LE LIG    +A+ VLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240
           ELLP+F+EPY SDSLQDFWGRRWNL++SRILR  ++DPCR L   V+G++PA ++AV+AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300
           F VSG+MHELIYFYMGR+APTWEV+ FF++HGVC+ AEMAV+   G RFRAPRV    LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTITLHFIPP 340
           + FVM T  WLFFPQ +R K DVRMLEEYAA+GAF+K +T   I P
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGP 346

[Close]

BLAST of CsGy3G027240 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 251.5 bits (641), Expect = 9.8e-67
Identity = 138/325 (42.46%), Postives = 189/325 (58.15%), Query Frame = 0


Query: 14  LVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQGPIGFFIGWLGSFK 73
           LV  SL   F I   +P G  + +L  P F +F  +P   +++H  G   FFI WL +FK
Sbjct: 10  LVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWLANFK 69

Query: 74  LLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIP---------DHKSPPNHSLIPPSAKL 133
           LLLFA G+GPL S     SLP FLAV  LPI+I           H+      LI  + K 
Sbjct: 70  LLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI-YTIKA 129

Query: 134 IFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELLPYFNEPY 193
           +F++L I A  +   L    +   Y + IYF LE ++  TA   + +  +EL P FN+PY
Sbjct: 130 VFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPY 189

Query: 194 FSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVVSGLMHEL 253
            + SLQDFWGRRWNLM + ILR T+Y+P   L   V+G   + ++A   TFVVSG+MHEL
Sbjct: 190 LATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSGIMHEL 249

Query: 254 IYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLTIMFVMGTGSW 313
           I+FYMGR+ P W++  FF+++G C   E+A++    GR+R P+     LT+ FVM T  W
Sbjct: 250 IFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALW 309

Query: 314 LFFPQCIRAKMDVRMLEEYAAIGAF 330
           LF P+  R  +  + L+EYAAIGAF
Sbjct: 310 LFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of CsGy3G027240 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 219.5 bits (558), Expect = 4.1e-57
Identity = 130/335 (38.81%), Postives = 183/335 (54.63%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+  L++       S+S C+     I +G  + L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++  +  P N   +P 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQ--MPK 120

Query: 121 ---SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGVELL 180
              + KL F  + +    +K H+ P  + + Y L IY   E L+     L    L  +L 
Sbjct: 121 WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLE 180

Query: 181 PYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIATFVV 240
           P+FNEPY S SLQDFWGRRWNLM   ILR  +Y P R +    +    A  + V A+F+V
Sbjct: 181 PHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLV 240

Query: 241 SGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV-RLGAGGRFRAPRVARICLTIM 300
           SG++HELI+FY  R +PT EVT FFV+HGVC  AE A  R     R++  ++    LT+ 
Sbjct: 241 SGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVG 300

Query: 301 FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLK 332
           FV+ TG WLFFP   R+ M  R+ +E      F+K
Sbjct: 301 FVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of CsGy3G027240 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-52
Identity = 129/343 (37.61%), Postives = 179/343 (52.19%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  +   L++       SLS C+ I + +  G L+ L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSP---PNHSL 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I    SP   PN   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 IPPSAKLIFLILTILAINFKNHLHPNAIPLF-----YCLLIYFFLEFLIGTTATLAKTVL 180
            P    ++ + + +L +    + + + +P F     YCL IY  +E ++     +  T+L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVI 240
           G  + P FNEPY + SLQDFW RRWNLM S +LR T++ P +     ++    A    V+
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARIC 300
           A+F VSGLMHEL+YFYM R  PTWEVTCFFV+HG     E+AV+       R P  A   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 LTIM-FVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLKTIT 335
           L ++ FV  TG WLF  Q +R  +  + + E   +    K  T
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFT 339

[Close]

BLAST of CsGy3G027240 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-52
Identity = 124/335 (37.01%), Postives = 184/335 (54.93%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+  L++       S+S C+ I + I  G L+ L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
             GFF  WL +FKL LFAF + PL  +   S+L RF      PI+I  +K+P ++ +   
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI--NKNPSSNRIHNK 120

Query: 121 SAKLIFLILTILAINFKNHLHPN---------AIPLFYCLLIYFFLEFLIGTTATLAKTV 180
                 L   +L  +F  H++ N         A    + + +Y   E ++     L   +
Sbjct: 121 PMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAV 240
           LG E+ P FN+PY + SLQ+FW RRWNLM   +LR  ++ P +     ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 IATFVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFR---APRV 300
           +ATF+VSGLMHELIYFY+ R +PTWEVTCFF++HGV    E+A++     R R    PR 
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 ARICLTI-MFVMGTGSWLFFPQCIRAKMDVRMLEE 323
           A   L I +F++ T  WLF+PQ +R  +  R++ E
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CsGy3G027240 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.9e-52
Identity = 129/340 (37.94%), Postives = 188/340 (55.29%), Query Frame = 0


Query: 1   MASEIHGLLQTLFLVSSSLSLCFSIRNSIPNGFLKFLLILPFFFLFLYIPLQFHTIHFQG 60
           M  E+  L++       S+S C+ I   I +G  +FL + P   LFL +PL F ++H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120
              FF+ WL +FKL+LF+F KGPL    T  + PRF      PI++  +    NH  +P 
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPT--NFPRFFCFTCFPIKVQQNPKSQNH--LP- 120

Query: 121 SAKLIFLI-LTILAI-----NFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180
             KL+F I L I A+     +++ +L P  +   Y + +Y  +E ++     +    LG 
Sbjct: 121 --KLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240
           +L P  N+PY + SLQDFWGRRWNLM   ILR  +Y P R ++   +    A    ++A 
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAV--RLGAGGRFR-APRVARI 300
           F+VSGL+HEL++FY+ R  PT EVT FFV+HGVC   E+AV  +     R+R +P V+R+
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRV 300

Query: 301 CLTIMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFLK 332
            LT+ FV  TG WLF PQ  R+ +  R   E   +  F+K
Sbjct: 301 -LTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

The following BLAST results are available for this feature:

BLAST of CsGy3G027240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.4e-65	42.46	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	1.4e-57	41.25	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	5.8e-56	38.81	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	1.5e-51	37.61	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ73	1.5e-51	37.01	Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX...	[more]

BLAST of CsGy3G027240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149743.1	1.08e-250	99.14	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus]	[more]
KAE8650923.1	1.47e-232	99.08	hypothetical protein Csa_001633 [Cucumis sativus]	[more]
XP_008461836.1	1.69e-219	88.79	PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418....	[more]
XP_038903196.1	8.48e-206	83.91	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_038905685.1	9.22e-175	72.70	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]

BLAST of CsGy3G027240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9R8	5.23e-251	99.14	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=...	[more]
A0A5D3BC71	8.17e-220	88.79	Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CG36	8.17e-220	88.79	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350034...	[more]
A0A6J1F144	7.31e-167	69.65	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IX47	1.47e-166	69.65	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CsGy3G027240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	9.8e-67	42.46	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	4.1e-57	38.81	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.0e-52	37.61	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	1.0e-52	37.01	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	8.9e-52	37.94	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 178..263 e-value: 9.3E-17 score: 61.1
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 3..334
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 3..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G027240.1	CsGy3G027240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity