CsGy3G024350 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G024350
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	AvrRpt-cleavage domain-containing protein
Location	Gy14Chr3: 24377103 .. 24378437 (-)
RNA-Seq Expression	CsGy3G024350
Synteny	CsGy3G024350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGGTAAGATTCTCTCTTCCTCTCCTTCTATTCTATTTCCAATGCTATCGAGTTTGATCTTCTCTGTATGAAGATTTTGCAAAGTTTTTTGGGGAATGTGAATTCTGATAACGTAACATGTCTTAAAAGAAAAAAAAGAAAAAAACAGATCTTTGTTATGGCTTAGCGATGGCATTTCTATATTAGAAACAGCATTTGCTTGTATCGACCATGCATTTTCGCATTCTATCAATCCAATTATGGATATAAATCTATAATATGGCTTAGCTATGATGTTTCGTATCATTTTCTGGTAGAAAATGGAATTTGTGAATTTGTTGCCTATATAACATTCAAAATCGGAAAGAATCTGGCATATATTTGGAAGCTATTGTTTTGTTTTTCCTCCGTTGTGGGAATGGTGATCAATGTATGATCAATGGGTTAGCTTCCATTAACTTCTCTCCCTGACATACCTGCCTTGTTTGATACCCACATCAGAAGAATCTATTTCATTGCTTTCATAGAAATGGATGGCCAAAAGCAAACACAAGATAGGAAGAGATCTACACATTAACATGGAACATTCTATGCAAGAGAAAAAGCAATGGACGAACGGACTAATATGGGAATTCACAAGTATAATTATGAATGAAAAATATCTGACTTCTTGTACTTTTCTAATGTCTATAGCAGGATAAAGGTCGCCCCTTGCCGAAGTTTGGCGAGTGGGATGTCAACAACCCAGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCAAATGAGGCTCCTGGGAACACAGCAGCAAAAGATAATACATCCAGACAAGAAGAAAATTATCAATATTCTCCCATGGTAAGTAATTTCACTAATTTTACTTATATTATATCATTGACTAAAACATTAGAAAACGCTGGCTCAATGATGAAAACATCCATTAATTCAAGGGTTTGTTATCGACATTAAATATCTATTGATATCTCATATATATTCTATAAATGTTCATAAAACATGAAAAACACCATCTACTCAGTTAATTACAAAAACTCAATGCTAACAACGATAACAATATCCATAATTGAGTTTGGAAGTAAAAGCTTAATTATGAATCTAAATAAACTGAATAAATTCTTTTATATTTACAAGAATATCCATCCATATCAATATCTTTTCGATATATCCATAAAATAGAAATTTCAATTTGATATCAACATCAATATTTTAATCCTAGACTCAACTTTGGCTTCATCTTCTTGATATTTGGTTAGAATTTTAACTACAAATTTTCGTGCTTGGATGCAGAAGAAATGGTTTTGTTGTTTCTGA

mRNA sequence

ATGGCTTCGGATAAAGGTCGCCCCTTGCCGAAGTTTGGCGAGTGGGATGTCAACAACCCAGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCAAATGAGGCTCCTGGGAACACAGCAGCAAAAGATAATACATCCAGACAAGAAGAAAATTATCAATATTCTCCCATGAAGAAATGGTTTTGTTGTTTCTGA

Coding sequence (CDS)

ATGGCTTCGGATAAAGGTCGCCCCTTGCCGAAGTTTGGCGAGTGGGATGTCAACAACCCAGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCAAATGAGGCTCCTGGGAACACAGCAGCAAAAGATAATACATCCAGACAAGAAGAAAATTATCAATATTCTCCCATGAAGAAATGGTTTTGTTGTTTCTGA

Protein sequence

MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYSPMKKWFCCF*

Homology

[Close]

BLAST of CsGy3G024350 vs. ExPASy Swiss-Prot
Match: O22633 (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.3e-15
Identity = 41/70 (58.57%), Postives = 50/70 (71.43%), Query Frame = 0


Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN--TAAKDNTSRQEENYQ 62
          SDKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+   +++ +      +  
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGKSSEGHVKSGGGDPS 61

Query: 63 YSPMKKWFCC 71
              KKW CC
Sbjct: 62 KPQPKKWLCC 71

[Close]

BLAST of CsGy3G024350 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.2e-05
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0


Query: 4   DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSN-EAPGNTAAKDNTSRQEENYQYS 63
           +K   +PKFG+WD NNP+SA+G+T IF+K R+E+ S     G++    + S +  N   S
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPTHQSSRNPNNTSS 202

Query: 64  PMKKWFCCF 72
                 CCF
Sbjct: 203 -----CCCF 206

[Close]

BLAST of CsGy3G024350 vs. NCBI nr
Match: KGN58221.1 (hypothetical protein Csa_017629 [Cucumis sativus])

HSP 1 Score: 154 bits (388), Expect = 3.82e-47
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPMKKWFCCF 71
          YSPMKKWFCCF
Sbjct: 61 YSPMKKWFCCF 71

[Close]

BLAST of CsGy3G024350 vs. NCBI nr
Match: XP_038883820.1 (protein NOI4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 143 bits (360), Expect = 9.05e-43
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          +  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AK+NTSR EENYQ
Sbjct: 9  VLQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQ 68

Query: 61 YSPMKKWFCCF 71
          YSPMKKWFCCF
Sbjct: 69 YSPMKKWFCCF 79

[Close]

BLAST of CsGy3G024350 vs. NCBI nr
Match: XP_038883812.1 (protein NOI4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 143 bits (360), Expect = 1.55e-42
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          +  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AK+NTSR EENYQ
Sbjct: 27 VLQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQ 86

Query: 61 YSPMKKWFCCF 71
          YSPMKKWFCCF
Sbjct: 87 YSPMKKWFCCF 97

[Close]

BLAST of CsGy3G024350 vs. NCBI nr
Match: KAG6581070.1 (Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141 bits (356), Expect = 5.75e-42
Identity = 64/71 (90.14%), Postives = 67/71 (94.37%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          +  D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK+N SR EENYQ
Sbjct: 24 LLPDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 83

Query: 61 YSPMKKWFCCF 71
          YSPMKKWFCCF
Sbjct: 84 YSPMKKWFCCF 94

[Close]

BLAST of CsGy3G024350 vs. NCBI nr
Match: KAG6588926.1 (Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129 bits (325), Expect = 1.63e-37
Identity = 62/71 (87.32%), Postives = 64/71 (90.14%), Query Frame = 0


Query: 1  MAS-DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENY 60
          MAS DKGRPLPKFGEWDVNNPASAEGFTVIFSKAR+EKKSNEA GN AAK+N    EENY
Sbjct: 1  MASQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARNEKKSNEASGNIAAKNNAPEPEENY 60

Query: 61 QYSPMKKWFCC 70
          QYSPMKKWFCC
Sbjct: 61 QYSPMKKWFCC 71

[Close]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL
Match: A0A0A0LDP9 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G595220 PE=4 SV=1)

HSP 1 Score: 154 bits (388), Expect = 1.85e-47
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPMKKWFCCF 71
          YSPMKKWFCCF
Sbjct: 61 YSPMKKWFCCF 71

[Close]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL
Match: A0A5D3E371 (RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00620 PE=4 SV=1)

HSP 1 Score: 129 bits (324), Expect = 2.25e-37
Identity = 61/63 (96.83%), Postives = 62/63 (98.41%), Query Frame = 0


Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 61
          + DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY
Sbjct: 19 SQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 78

Query: 62 SPM 64
          SPM
Sbjct: 79 SPM 81

[Close]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL
Match: A0A5J5AK52 (AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004861 PE=4 SV=1)

HSP 1 Score: 114 bits (286), Expect = 7.88e-32
Identity = 56/74 (75.68%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA--PGNTAAK-DNTSRQEEN 61
          + DKGRPLPKFGEWDVNNPASA+GFTVIF+KARD+KK+N A  PG  + K DNTS+Q EN
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASADGFTVIFAKARDDKKTNGAAGPGGPSQKNDNTSKQNEN 62

Query: 62 YQYSPM-KKWFCCF 71
          YQY PM KKWFCCF
Sbjct: 63 YQYPPMEKKWFCCF 76

[Close]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL
Match: A0A5J5BYY8 (AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004561 PE=4 SV=1)

HSP 1 Score: 109 bits (273), Expect = 8.48e-30
Identity = 53/78 (67.95%), Postives = 61/78 (78.21%), Query Frame = 0


Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAP-------GNTAAKDNTSR 61
          + DKGRPLPKFGEWDVNNPASA+GFTVIF+KARD+KK+N A        G    K++ S+
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASADGFTVIFAKARDDKKTNGAGAGAGGGGGGPTQKNDNSK 62

Query: 62 QEENYQYSPM-KKWFCCF 71
          Q ENYQYSPM KKWFCCF
Sbjct: 63 QNENYQYSPMEKKWFCCF 80

[Close]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL
Match: A0A2P5B5J0 (RIN4, pathogenic type III effector avirulence factor Avr cleavage site OS=Parasponia andersonii OX=3476 GN=PanWU01x14_269930 PE=4 SV=1)

HSP 1 Score: 108 bits (271), Expect = 1.62e-29
Identity = 57/78 (73.08%), Postives = 58/78 (74.36%), Query Frame = 0


Query: 1  MAS-DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK---DNTSRQE 60
          MAS DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSN A G+       DN S   
Sbjct: 1  MASQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNGAAGSGTTNQKNDNNSANN 60

Query: 61 ---ENYQYSPMKKWFCCF 71
             E Y Y PMKKWFCCF
Sbjct: 61 KPGETYDYPPMKKWFCCF 78

[Close]

BLAST of CsGy3G024350 vs. TAIR 10
Match: AT5G40645.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 83.6 bits (205), Expect = 7.2e-17
Identity = 44/67 (65.67%), Postives = 51/67 (76.12%), Query Frame = 0


Query: 7  RPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAA--KDNTSRQEENYQYSPM 66
          RPLPKFGEWDVN+PASAEGFTVIF+KARD+KK+N A G  A+  +DN   Q+E     P 
Sbjct: 13 RPLPKFGEWDVNDPASAEGFTVIFAKARDDKKTN-ASGRAASQRRDNNKSQDE-----PT 72

Query: 67 KKWFCCF 72
          KK FCCF
Sbjct: 73 KKRFCCF 73

[Close]

BLAST of CsGy3G024350 vs. TAIR 10
Match: AT5G63270.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 83.6 bits (205), Expect = 7.2e-17
Identity = 42/78 (53.85%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA----PGNTAAKDNTSRQE 60
          MA +KG+PLPKFGEWDVNNPASAEGFTVIFSKA DEKK+ +A    P +  +       +
Sbjct: 1  MAENKGKPLPKFGEWDVNNPASAEGFTVIFSKASDEKKTKKASGAGPNSLVSPQRNQNSD 60

Query: 61 ENYQYSPM-----KKWFC 70
          +N  +S        KWFC
Sbjct: 61 QNNNHSSQNPKAKNKWFC 78

[Close]

BLAST of CsGy3G024350 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.6e-16
Identity = 38/68 (55.88%), Postives = 46/68 (67.65%), Query Frame = 0


Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYS 62
          +DKGRPLPKFGEWDVN+P+SAEGFTVIF+KAR+EKK      +    +    +       
Sbjct: 2  ADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGGKSDSPGKDEPGYNKNGEVLEK 61

Query: 63 PMKKWFCC 71
          P KKWFCC
Sbjct: 62 PAKKWFCC 69

[Close]

BLAST of CsGy3G024350 vs. TAIR 10
Match: AT5G55850.3 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.8e-16
Identity = 40/69 (57.97%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 4  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN--TAAKDNTSRQEENYQY 63
          DKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+   +++ +      +   
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGKSSEGHVKSGGGDPSK 78

Query: 64 SPMKKWFCC 71
             KKW CC
Sbjct: 79 PQPKKWLCC 87

[Close]

BLAST of CsGy3G024350 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-15
Identity = 36/43 (83.72%), Postives = 40/43 (93.02%), Query Frame = 0


Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          SDKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 44

The following BLAST results are available for this feature:

BLAST of CsGy3G024350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22633	1.3e-15	58.57	Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1	[more]
Q8GYN5	1.2e-05	39.13	RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1	[more]

BLAST of CsGy3G024350 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN58221.1	3.82e-47	100.00	hypothetical protein Csa_017629 [Cucumis sativus]	[more]
XP_038883820.1	9.05e-43	91.55	protein NOI4-like isoform X2 [Benincasa hispida]	[more]
XP_038883812.1	1.55e-42	91.55	protein NOI4-like isoform X1 [Benincasa hispida]	[more]
KAG6581070.1	5.75e-42	90.14	Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6588926.1	1.63e-37	87.32	Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CsGy3G024350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDP9	1.85e-47	100.00	AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G59...	[more]
A0A5D3E371	2.25e-37	96.83	RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A5J5AK52	7.88e-32	75.68	AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0...	[more]
A0A5J5BYY8	8.48e-30	67.95	AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0...	[more]
A0A2P5B5J0	1.62e-29	73.08	RIN4, pathogenic type III effector avirulence factor Avr cleavage site OS=Parasp...	[more]

BLAST of CsGy3G024350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40645.1	7.2e-17	65.67	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G63270.1	7.2e-17	53.85	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT2G04410.1	1.6e-16	55.88	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.3	2.8e-16	57.97	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.1	1.8e-15	83.72	RPM1-interacting protein 4 (RIN4) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008700	RIN4, pathogenic type III effector avirulence factor Avr cleavage site	PFAM	PF05627	AvrRpt-cleavage	coord: 4..39 e-value: 2.4E-23 score: 81.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..59
None	No IPR available	PANTHER	PTHR33159:SF69	RPM1-INTERACTING PROTEIN 4-LIKE	coord: 1..71
IPR040387	RPM1-interacting protein 4/NOI4	PANTHER	PTHR33159	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G024350.2	CsGy3G024350.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane