CsGy3G005290 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G005290
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionfatty acid desaturase 4, chloroplastic
LocationGy14Chr3: 4213660 .. 4214601 (+)
RNA-Seq ExpressionCsGy3G005290
SyntenyCsGy3G005290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTTATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTACTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACAAAGCGACAGTTTGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

mRNA sequence

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTTATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTACTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACAAAGCGACAGTTTGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Coding sequence (CDS)

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTTATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTACTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACAAAGCGACAGTTTGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Protein sequence

MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEILEQTTAH*
Homology
BLAST of CsGy3G005290 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.4e-108
Identity = 185/292 (63.36%), Postives = 223/292 (76.37%), Query Frame = 0

Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   SH+ LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

BLAST of CsGy3G005290 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 6.5e-99
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   SH+WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI G Q+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

BLAST of CsGy3G005290 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.7e-97
Identity = 168/291 (57.73%), Postives = 213/291 (73.20%), Query Frame = 0

Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  SH+WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

BLAST of CsGy3G005290 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.9e-18
Identity = 64/199 (32.16%), Postives = 88/199 (44.22%), Query Frame = 0

Query: 99  ISLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAF 158
           ++L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G   I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 159 QGHHKWPWTITKRQFA-----NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMF 218
           + HH     IT+  F      N L +L  A+    LP+S          F+G     +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 219 SQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDET 278
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  NK L   
Sbjct: 162 TNQFHKWSHMDSP--PALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 279 KLFEALEMILYFKLGLRPR 292
             F   E ++ +  GL PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

BLAST of CsGy3G005290 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.0e-12
Identity = 48/179 (26.82%), Postives = 79/179 (44.13%), Query Frame = 0

Query: 115 LEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAFQGHHKWPWTITKRQFA 174
           ++  +  ++GY+L+D   G+YHW  D Y    TPI G T I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 175 NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVA 234
           + +   A+     + P+   F    L  F+      I  +   H + H    E P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 235 MQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRS 293
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  L+  LE  + +  G++PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

BLAST of CsGy3G005290 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 649 bits (1675), Expect = 1.90e-235
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 623 bits (1607), Expect = 4.42e-225
Identity = 298/313 (95.21%), Postives = 305/313 (97.44%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 621 bits (1602), Expect = 2.56e-224
Identity = 296/313 (94.57%), Postives = 304/313 (97.12%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 609 bits (1570), Expect = 2.24e-219
Identity = 290/317 (91.48%), Postives = 303/317 (95.58%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVA----TSPKHADGQLVIERE 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRV YCIAN+TVN  A    T+PKHADGQL+IERE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGATTTNPKHADGQLLIERE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLE 120
           VISPTPLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASSHIWLE
Sbjct: 61  VISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           PALAALVGYILADLGSGVYHWGIDNYG+ STPIFGTQIEAFQGHHKWPWTITKRQFANN+
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANNM 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTFAVLPVSLAFDDPILHGFVG+CSGCIMFSQQFHAWAH KKSELPPLVVAMQ+
Sbjct: 181 YALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWNK+LDETK+FEALEMIL+FKLG+RPRSWTEP
Sbjct: 241 AGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTEP 300

Query: 301 NSEWTEETEILEQTTAH 313
           NS WTEETEI+EQTTAH
Sbjct: 301 NSAWTEETEIIEQTTAH 317

BLAST of CsGy3G005290 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 558 bits (1438), Expect = 2.83e-199
Identity = 265/316 (83.86%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTT----VNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASSHIWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 312
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

BLAST of CsGy3G005290 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 649 bits (1675), Expect = 9.18e-236
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 623 bits (1607), Expect = 2.14e-225
Identity = 298/313 (95.21%), Postives = 305/313 (97.44%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 1.24e-224
Identity = 296/313 (94.57%), Postives = 304/313 (97.12%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 313
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of CsGy3G005290 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 558 bits (1438), Expect = 1.37e-199
Identity = 265/316 (83.86%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTT----VNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASSHIWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 312
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

BLAST of CsGy3G005290 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 547 bits (1409), Expect = 3.86e-195
Identity = 263/318 (82.70%), Postives = 283/318 (88.99%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATS-----PKHADGQLVIER 60
           MSIL QNKFLLSS++HV+  S  +PR+ V CIANTTVN  A +     PKH DGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWL 120
           E +SPT     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAASSH WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240
           LYALARAVTF VLP++LA +DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDETK+FEALEMIL+FKLG+RPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 313
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

BLAST of CsGy3G005290 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 392.9 bits (1008), Expect = 2.4e-109
Identity = 185/292 (63.36%), Postives = 223/292 (76.37%), Query Frame = 0

Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   SH+ LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

BLAST of CsGy3G005290 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 362.1 bits (928), Expect = 4.6e-100
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0

Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   SH+WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI G Q+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

BLAST of CsGy3G005290 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 356.7 bits (914), Expect = 1.9e-98
Identity = 168/291 (57.73%), Postives = 213/291 (73.20%), Query Frame = 0

Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  SH+WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ423.4e-10863.36Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P... [more]
O045846.5e-9959.15Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
O810062.7e-9757.73Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9AE871.9e-1832.16Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1[more]
Q5UR786.0e-1226.82Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=... [more]
Match NameE-valueIdentityDescription
XP_004152938.11.90e-23599.68fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica... [more]
KAA0033959.14.42e-22595.21fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu... [more]
XP_008463431.12.56e-22494.57PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo][more]
XP_038905900.12.24e-21991.48fatty acid desaturase 4, chloroplastic-like [Benincasa hispida][more]
XP_022150986.12.83e-19983.86fatty acid desaturase 4, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0L6B99.18e-23699.68TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07... [more]
A0A5A7SXQ72.14e-22595.21Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CJN91.24e-22494.57fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P... [more]
A0A6J1DD701.37e-19983.86fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1IGF13.86e-19582.70fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767... [more]
Match NameE-valueIdentityDescription
AT4G27030.12.4e-10963.36fatty acid desaturase A [more]
AT1G62190.14.6e-10059.15Kua-ubiquitin conjugating enzyme hybrid localisation domain [more]
AT2G22890.11.9e-9857.73Kua-ubiquitin conjugating enzyme hybrid localisation domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019547B domain of TMEM189, localisation domainPFAMPF10520TMEM189_B_dmaincoord: 125..293
e-value: 1.3E-64
score: 217.4
NoneNo IPR availablePANTHERPTHR48140FAMILY NOT NAMEDcoord: 1..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G005290.1CsGy3G005290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0016021 integral component of membrane