CsGy2G008650 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G008650
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionprotein PHYTOCHROME KINASE SUBSTRATE 1
LocationGy14Chr2: 7737623 .. 7739350 (+)
RNA-Seq ExpressionCsGy2G008650
SyntenyCsGy2G008650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTATTTACCACTCTTTGATCAGCTCTCTGTCTTCTCTTTGGTGTCTCCTTTTGTTTTGGGATCCAATTTTCGAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTATCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGATAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGAGATGATTGCATGTTATCTTCATATTTCCATAAGTACATTAAACATTTGAACTATACTTATTTACTCTACTCTACTATAAAATAGGATATTTTCCTATTCACTGCTATTCCAAATTTGAATATACCTTTGGTACGAGTGAGGGGTATGTATACATTATTATTCTAAACTCTCCTG

mRNA sequence

TTATTATTTACCACTCTTTGATCAGCTCTCTGTCTTCTCTTTGGTGTCTCCTTTTGTTTTGGGATCCAATTTTCGAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTATCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGATAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGAGATGATTGCATGTTATCTTCATATTTCCATAAGTACATTAAACATTTGAACTATACTTATTTACTCTACTCTACTATAAAATAGGATATTTTCCTATTCACTGCTATTCCAAATTTGAATATACCTTTGGTACGAGTGAGGGGTATGTATACATTATTATTCTAAACTCTCCTG

Coding sequence (CDS)

ATGGACATCTTCACCTCCATATCATCTAAAACATTGCCTTTTGATACCCATATTGATAGCAATAATAGTTTGGGAGTTTATGGCGACACCTCCTTCTCGTCTTACTTATCTGCGAAAGAAGACCATGACTTCATCCGCAAACTCGCTGAGTCAACTCGTTACCTAAAAAGCCCCAACATGATTCCTTGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGACGGAATGGAAGATGAGAGCACTCAAAGATCAAGTAACAATCATCCATCGTCTCAAAAGCTTGATAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAATTGCCAAAGCCGAGGCTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGCCAAAGACCTTTGCTGAAGATCGTTAAAAGCACAACAACAACAAGTGCAATAGCAACAACAATTGCCAGTAACAGTTATTCGCTCCAGAAGAGGAGTAGTAACAACAACAAGAGCTTTCTATCCAATACACTTGGCTATTGCATGTGCTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGTGAAATCAGTTTCAGCAATGCAATCACAACAAACCCAACACGAAATAATATTAATAATATATTGGATCGAGAAGCGCCTTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTACAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCGTTAGAGGTTTTCGGTTCGCCTATGACGGGAAGGCTCAGAAACAATAAACCAATGAGCCTGGAAAAAAGGCTAACCATGCTTTCTTGGGATAAAACAAATAACCACAGCACCACCTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTTGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCGTCACTGCCAGTGCTCTAGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCAATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTCCCGAAGAGGCGACCGAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGTAATAATAAGTATGAACGCAAAATGGGTGGTAACAAGTCTAACAACTCTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTCGGAGGGTTCAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTATTCCCCTGCCTCTGCCACATCATGGTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATAACCTTCAATATGCAGTGA

Protein sequence

MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSPRLTNITFNMQ*
Homology
BLAST of CsGy2G008650 vs. ExPASy Swiss-Prot
Match: Q9SWI1 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 155/445 (34.83%), Postives = 216/445 (48.54%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N+   +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFN  M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIASNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKS 420
           VTASA DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

BLAST of CsGy2G008650 vs. ExPASy Swiss-Prot
Match: Q9M9T4 (Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.2e-28
Identity = 148/432 (34.26%), Postives = 209/432 (48.38%), Query Frame = 0

Query: 5   TSISSKTLPFDTHI-DSNNSLGVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  + ++NNS  +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFN  M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    I T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRLRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----I 402
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR SS    +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 395

BLAST of CsGy2G008650 vs. ExPASy Swiss-Prot
Match: Q8GXS8 (Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 PE=2 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 1.6e-11
Identity = 112/382 (29.32%), Postives = 158/382 (41.36%), Query Frame = 0

Query: 65  RGGEDGEIGIFGAEKYFN------DGMEDESTQRSSNN---HPSSQKLDKLIVAHMEEAL 124
           +   D EIG+FGAEKYF+      D   D + Q    N   HP++   +     H     
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANP-NPNPNPHPHPHP 100

Query: 125 KLPK--------PRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR 184
           +L K         R GTPSV SE SS NSQ  L++I                N+++  K+
Sbjct: 101 QLTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI----------------NNHNENKQ 160

Query: 185 SSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNP-----TRNNINNILDR 244
              N+ S    + G   C             G  S    + T+P     +RN+  + +  
Sbjct: 161 RKMNDTSI---SFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAY 220

Query: 245 EAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 304
           +A      P      K       E++    SL +         R++  M+LE++L++L+W
Sbjct: 221 DARKHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTW 280

Query: 305 D--------KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASC 364
           D        K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S 
Sbjct: 281 DAIPNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSV 340

Query: 365 YAPSEASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRR 407
           Y PSEAS+ WSVVT S        DFD  +  T +   V   P          V+  K R
Sbjct: 341 YEPSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVR 357

BLAST of CsGy2G008650 vs. NCBI nr
Match: XP_004138989.1 (protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetical protein Csa_006559 [Cucumis sativus])

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 490
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

BLAST of CsGy2G008650 vs. NCBI nr
Match: XP_008441702.1 (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa] >TYK13512.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa])

HSP 1 Score: 875 bits (2261), Expect = 0.0
Identity = 455/491 (92.67%), Postives = 468/491 (95.32%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR-SSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQKR SSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLP+  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 490
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of CsGy2G008650 vs. NCBI nr
Match: XP_038889450.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida])

HSP 1 Score: 659 bits (1699), Expect = 2.45e-233
Identity = 381/510 (74.71%), Postives = 420/510 (82.35%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNS-LGVYGDTSFSSYLSAKEDHDFI-RKLAESTRYLKSP 60
           MDIF+SIS+KTLPFDTHID+NN+ +GVYGD S     S KEDH FI  KL ESTRYLKS 
Sbjct: 1   MDIFSSISTKTLPFDTHIDNNNNNIGVYGDPS-----SYKEDHTFIIHKLTESTRYLKSS 60

Query: 61  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIV--AH-----M 120
           N IP S G EDGEIGIFGAEKYFN G+E++ T+       S+QKLDKL+V  AH     +
Sbjct: 61  NTIPGSGGHEDGEIGIFGAEKYFNGGIENDGTRTER----SAQKLDKLLVLAAHQQHNLL 120

Query: 121 EEALKLPKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSS-- 180
           EE+LKLPKPRLGTPSVGSESSS+NSQRPLL +VK+       +TTIA+N+YSLQKRSS  
Sbjct: 121 EESLKLPKPRLGTPSVGSESSSINSQRPLLNVVKN-------STTIANNNYSLQKRSSSS 180

Query: 181 NNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTR-----NNINNILDREA 240
           N+NKSFLSNT GYCMCC+S++KS AVEDVGEISFSNA+TT PTR     NN NNI +RE 
Sbjct: 181 NHNKSFLSNTFGYCMCCSSSEKS-AVEDVGEISFSNAVTTKPTRSNNNNNNNNNIFERET 240

Query: 241 PSFRGFPTAASS--LKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 300
           PSFRGFPTAASS  LKM+H QEPEEV ERKSLEVFGSP+ GRLRNNKP+SLEKRL MLSW
Sbjct: 241 PSFRGFPTAASSSSLKMIHFQEPEEVGERKSLEVFGSPVMGRLRNNKPISLEKRLAMLSW 300

Query: 301 DKTNNHSTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360
           D TNN++ +LGSG+FYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS
Sbjct: 301 DHTNNNNNSLGSGVFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWS 360

Query: 361 VVTASALDFDERRPSTTSPARVVPPP-PMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVA 420
           VVTASALDFDERR STTSP RVV P  PMRVNV+KEVV   KRRPSSILGCKSEKAVRVA
Sbjct: 361 VVTASALDFDERRLSTTSPTRVVAPSLPMRVNVNKEVVQ--KRRPSSILGCKSEKAVRVA 420

Query: 421 EGNNKYERKMGGNKSNNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSS-SMLPI 480
           E NNKY RKM G KSN  DY      NSESL+AVKR EDETKVGG SF+SQGSS S+LP+
Sbjct: 421 EDNNKYGRKMNG-KSNYCDY------NSESLVAVKRSEDETKVGGLSFRSQGSSASLLPL 480

Query: 481 PLPLPHHGLATRSLPRPYSPRLTNITFNMQ 490
           P P PH  LATR LPRPYSPRLTNI+FN+Q
Sbjct: 481 PPP-PHRALATRPLPRPYSPRLTNISFNIQ 483

BLAST of CsGy2G008650 vs. NCBI nr
Match: XP_022998592.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima])

HSP 1 Score: 343 bits (880), Expect = 1.08e-110
Identity = 233/432 (53.94%), Postives = 270/432 (62.50%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS   LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKPSKN-LD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGY-C 189
           V SES  ++ N+Q+PLL   K+                       +N K FLS+ LGY C
Sbjct: 76  VASESCTTTTNTQKPLLNTHKT-----------------------HNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFR--GFPTAASSLKMVH 249
           MC + + K T  +D+GEISFS  +TT PT             SFR   F     SLK+VH
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAPTA------------SFRDNSFTFQNGSLKLVH 195

Query: 250 LQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNED 309
            QEPE  VERKSLEVFGSP+ GR RN KP SLEK+L MLSWD TN+ S         N D
Sbjct: 196 FQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNSSSNN-------NND 255

Query: 310 EVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSP 369
           E NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSP
Sbjct: 256 EANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSP 315

Query: 370 ARVVPPPP-MRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 429
           ARV PPPP  RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+ G  + NS
Sbjct: 316 ARVAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNS 375

Query: 430 DYLLMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPY 489
               +      S+ ++V R EDET+V  FSF+SQ    +LP      HH LATRSLPRPY
Sbjct: 376 SSSSVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPLLPSS----HHALATRSLPRPY 387

BLAST of CsGy2G008650 vs. NCBI nr
Match: XP_022949325.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata])

HSP 1 Score: 336 bits (862), Expect = 6.70e-108
Identity = 227/433 (52.42%), Postives = 265/433 (61.20%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS       I    +E    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS-------INLAPDEPFT-PNPWLATPS 75

Query: 130 VGSES---SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGY- 189
           V SES   ++  +Q+PLL   K+                       +N K FLS+ LGY 
Sbjct: 76  VASESCTTTTTTTQKPLLNTHKT-----------------------HNTKGFLSHALGYY 135

Query: 190 CMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFR--GFPTAASSLKMV 249
           CMC + + K +  +D+GEISFS  +TT PT             SFR   F     SLK+V
Sbjct: 136 CMCYSRSDKPS--DDIGEISFSRPVTTAPTA------------SFRDNSFTFQNGSLKLV 195

Query: 250 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 309
           H QEPE  VERKSLEVFGSP+ GR RN KP SLEK+L MLSWD TNN++        YN 
Sbjct: 196 HFQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNNNN--------YNN 255

Query: 310 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 369
           DE NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTS
Sbjct: 256 DEANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTS 315

Query: 370 PARVV---PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKS 429
           PARV    PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   +  G    
Sbjct: 316 PARVAAPPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNF 375

Query: 430 NNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPR 489
           N+S   +         ++V RFEDET+V  FSF+SQ    +LP       H LATRSLPR
Sbjct: 376 NSSSSSVSVSVPVPVSVSVNRFEDETRVRSFSFRSQAPPPLLPSS----RHSLATRSLPR 388

BLAST of CsGy2G008650 vs. ExPASy TrEMBL
Match: A0A0A0LKV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1)

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300
           HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNMQ 490
           RLTNITFNMQ
Sbjct: 481 RLTNITFNMQ 490

BLAST of CsGy2G008650 vs. ExPASy TrEMBL
Match: A0A5A7SNC2 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00080 PE=4 SV=1)

HSP 1 Score: 875 bits (2261), Expect = 0.0
Identity = 455/491 (92.67%), Postives = 468/491 (95.32%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR-SSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQKR SSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLP+  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 490
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of CsGy2G008650 vs. ExPASy TrEMBL
Match: A0A1S3B4P0 (protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 PE=4 SV=1)

HSP 1 Score: 875 bits (2261), Expect = 0.0
Identity = 455/491 (92.67%), Postives = 468/491 (95.32%), Query Frame = 0

Query: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60
           MDIFTSISSKTLPFDTHIDSNN+LGVYGDTSFSSYL+AKEDHDFIRKL ESTRYLKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQRS NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR-SSNNNKSFLSNT 180
           RLGTPSVGSESSSVNSQRPLLKIVKSTTTT+AIATTIA+NSYSLQKR SSNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNA+TTNPTRNNINNILDRE PSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYN 300
           VHLQEPEEVVERKSLEVFGSP+TGRLR+NKP+SLEKRLTMLSWDKTNNHSTTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KM GNKSNNS
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGG SFKSQGSSSMLP+  P     L TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNMQ 490
           PRLTN+TFNMQ
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of CsGy2G008650 vs. ExPASy TrEMBL
Match: A0A6J1K8F0 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493179 PE=4 SV=1)

HSP 1 Score: 343 bits (880), Expect = 5.22e-111
Identity = 233/432 (53.94%), Postives = 270/432 (62.50%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS   LD        +    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKPSKN-LD-------PDGPFTPNPWLATPS 75

Query: 130 VGSES--SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGY-C 189
           V SES  ++ N+Q+PLL   K+                       +N K FLS+ LGY C
Sbjct: 76  VASESCTTTTNTQKPLLNTHKT-----------------------HNTKGFLSHALGYYC 135

Query: 190 MCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFR--GFPTAASSLKMVH 249
           MC + + K T  +D+GEISFS  +TT PT             SFR   F     SLK+VH
Sbjct: 136 MCYSRSDKPT--DDIGEISFSKPVTTAPTA------------SFRDNSFTFQNGSLKLVH 195

Query: 250 LQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNED 309
            QEPE  VERKSLEVFGSP+ GR RN KP SLEK+L MLSWD TN+ S         N D
Sbjct: 196 FQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNSSSNN-------NND 255

Query: 310 EVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTSP 369
           E NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTSP
Sbjct: 256 EANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSP 315

Query: 370 ARVVPPPP-MRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNS 429
           ARV PPPP  RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+ G  + NS
Sbjct: 316 ARVAPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNS 375

Query: 430 DYLLMEQRNSESL-MAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPY 489
               +      S+ ++V R EDET+V  FSF+SQ    +LP      HH LATRSLPRPY
Sbjct: 376 SSSSVSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPLLPSS----HHALATRSLPRPY 387

BLAST of CsGy2G008650 vs. ExPASy TrEMBL
Match: A0A6J1GCF6 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452711 PE=4 SV=1)

HSP 1 Score: 336 bits (862), Expect = 3.24e-108
Identity = 227/433 (52.42%), Postives = 265/433 (61.20%), Query Frame = 0

Query: 70  GEIGIFGAEKYFNDGME---DESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKPRLGTPS 129
           GEIG+F AEKYFN G++   D       NN PS       I    +E    P P L TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS-------INLAPDEPFT-PNPWLATPS 75

Query: 130 VGSES---SSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTLGY- 189
           V SES   ++  +Q+PLL   K+                       +N K FLS+ LGY 
Sbjct: 76  VASESCTTTTTTTQKPLLNTHKT-----------------------HNTKGFLSHALGYY 135

Query: 190 CMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFR--GFPTAASSLKMV 249
           CMC + + K +  +D+GEISFS  +TT PT             SFR   F     SLK+V
Sbjct: 136 CMCYSRSDKPS--DDIGEISFSRPVTTAPTA------------SFRDNSFTFQNGSLKLV 195

Query: 250 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 309
           H QEPE  VERKSLEVFGSP+ GR RN KP SLEK+L MLSWD TNN++        YN 
Sbjct: 196 HFQEPE--VERKSLEVFGSPVIGRHRN-KPFSLEKKLAMLSWDHTNNNN--------YNN 255

Query: 310 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 369
           DE NSDCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSVVTASALDFDERR STTS
Sbjct: 256 DEANSDCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTS 315

Query: 370 PARVV---PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKS 429
           PARV    PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   +  G    
Sbjct: 316 PARVAAPPPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSNGRKISGKVNF 375

Query: 430 NNSDYLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPR 489
           N+S   +         ++V RFEDET+V  FSF+SQ    +LP       H LATRSLPR
Sbjct: 376 NSSSSSVSVSVPVPVSVSVNRFEDETRVRSFSFRSQAPPPLLPSS----RHSLATRSLPR 388

BLAST of CsGy2G008650 vs. TAIR 10
Match: AT2G02950.1 (phytochrome kinase substrate 1 )

HSP 1 Score: 139.4 bits (350), Expect = 7.6e-33
Identity = 155/445 (34.83%), Postives = 216/445 (48.54%), Query Frame = 0

Query: 5   TSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDH-DFIRKLAESTRYLK------- 64
           +S S+    FD   ++N+   +Y   S SSYLS+KED     +KL E ++ L        
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ---SPNMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFN  M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRLGTPSVGSESSSVNSQRPLL--KIVKSTTTTSAIATTIASNSYSLQKRSSNN 184
           A    K   GTPSV SE SS NSQ  LL  K+V S  +    +     NS    ++ +NN
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNS----SFKEKKNSNGQIQKVTNN 186

Query: 185 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
            KSFL+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         
Sbjct: 187 KKSFLAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS-------- 246

Query: 245 TAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTT 304
           +A  + +++ +Q+ EE+ +RKSLEVFGSP+       K   ++K+L +  W        T
Sbjct: 247 SADMNTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDT 306

Query: 305 LGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSV 364
              G         SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS+
Sbjct: 307 KSEG---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSI 366

Query: 365 VTASALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKS 420
           VTASA DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS
Sbjct: 367 VTASAADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKS 402

BLAST of CsGy2G008650 vs. TAIR 10
Match: AT1G14280.1 (phytochrome kinase substrate 2 )

HSP 1 Score: 127.9 bits (320), Expect = 2.3e-29
Identity = 148/432 (34.26%), Postives = 209/432 (48.38%), Query Frame = 0

Query: 5   TSISSKTLPFDTHI-DSNNSLGVYGDTSFS----SYLSAKEDHDFIRKLAES-TRYLKSP 64
           +S S+  + FD  + ++NNS  +YG  S S    SYL++KED    + L    T  +   
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITNDVLGI 65

Query: 65  NMIPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQ-KLDKLIVAHMEEALKL 124
           N     +  ED EI +FGAEKYFN  M+ + + R  +  P  +  ++++ V   + +   
Sbjct: 66  N----KKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS--- 125

Query: 125 PKPRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNN----- 184
            K    TPS+ SE SS NSQ  LL+  K       I    + NSY  +K  S+N+     
Sbjct: 126 -KNSSETPSLRSE-SSWNSQSLLLQ-SKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKST-AVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFP 244
           KSFL+ TLG C C  SN  S   V+D    S    I T                    F 
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRSSGLKKIKTQ-----------------LSFS 245

Query: 245 TAASSLKMVHLQEPEEVVE-RKSLEVFGSPMTGRLRNNKPMSLEKRLTM--LSWDKTNN- 304
              SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++ 
Sbjct: 246 GDLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSA 305

Query: 305 HSTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEAS 364
                G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S
Sbjct: 306 KKEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVS 365

Query: 365 VDWSVVTASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----I 402
           + WSVVTAS  DF       TSP +      +P  P+    ++E  + P+RR SS    +
Sbjct: 366 IQWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLL 395

BLAST of CsGy2G008650 vs. TAIR 10
Match: AT1G18810.1 (phytochrome kinase substrate-related )

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-12
Identity = 112/382 (29.32%), Postives = 158/382 (41.36%), Query Frame = 0

Query: 65  RGGEDGEIGIFGAEKYFN------DGMEDESTQRSSNN---HPSSQKLDKLIVAHMEEAL 124
           +   D EIG+FGAEKYF+      D   D + Q    N   HP++   +     H     
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANP-NPNPNPHPHPHP 100

Query: 125 KLPK--------PRLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKR 184
           +L K         R GTPSV SE SS NSQ  L++I                N+++  K+
Sbjct: 101 QLTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMRI----------------NNHNENKQ 160

Query: 185 SSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAITTNP-----TRNNINNILDR 244
              N+ S    + G   C             G  S    + T+P     +RN+  + +  
Sbjct: 161 RKMNDTSI---SFGGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAY 220

Query: 245 EAPSFRGFPTAASSLKMVHLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSW 304
           +A      P      K       E++    SL +         R++  M+LE++L++L+W
Sbjct: 221 DARKHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTW 280

Query: 305 D--------KTNNHSTT-LGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASC 364
           D        K NNH+     S M  N  E+E  S  SSDLFEIE++          T+S 
Sbjct: 281 DAIPNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSV 340

Query: 365 YAPSEASVDWSVVTASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRR 407
           Y PSEAS+ WSVVT S        DFD  +  T +   V   P          V+  K R
Sbjct: 341 YEPSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVR 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWI11.1e-3134.83Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 P... [more]
Q9M9T43.2e-2834.26Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 P... [more]
Q8GXS81.6e-1129.32Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 P... [more]
Match NameE-valueIdentityDescription
XP_004138989.10.0100.00protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetica... [more]
XP_008441702.10.092.67PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 p... [more]
XP_038889450.12.45e-23374.71protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida][more]
XP_022998592.11.08e-11053.94protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima][more]
XP_022949325.16.70e-10852.42protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LKV20.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1[more]
A0A5A7SNC20.092.67Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B4P00.092.67protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 P... [more]
A0A6J1K8F05.22e-11153.94protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GCF63.24e-10852.42protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G02950.17.6e-3334.83phytochrome kinase substrate 1 [more]
AT1G14280.12.3e-2934.26phytochrome kinase substrate 2 [more]
AT1G18810.11.1e-1229.32phytochrome kinase substrate-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33781:SF4PROTEIN PHYTOCHROME KINASE SUBSTRATE 1coord: 3..448
IPR039615Protein PHYTOCHROME KINASE SUBSTRATEPANTHERPTHR33781PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATEDcoord: 3..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G008650.1CsGy2G008650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009638 phototropism
molecular_function GO:0016301 kinase activity