CsGy1G011900 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G011900
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionlactosylceramide 4-alpha-galactosyltransferase
LocationGy14Chr1: 7502133 .. 7503451 (-)
RNA-Seq ExpressionCsGy1G011900
SyntenyCsGy1G011900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGATTGGTAATTTCAACCCTCTCAAGGAATTTACTTTCCATTTTCTCTCATCCCTTCCCACTTCTTTTCTTTCTCTTCTCCTTCTCTTCCTCTTGGCTTACAACGGCCTTCATGTTTTCTCTATTTCCCCACCCTCACCGGAGAAGACAATTCCTCATCCCGCTCCTTTCTCGCCCCAAAAACGACCCACTGCCGATTCCACTTCAACCCATCTTCTGTTTTCCATTCATGAAAACCATCCACCACCGGTCTTAAAATCAAATACCTCTGTTTTTCATCATCCCCACCTCAAATCCCGGCCCGCAAAGAGAGTCGGGAAGCATAAGCGCCGCCTGCGAAGTCTCCGGTCGGAATTGAAAGAAAGCGACTTTTCAGCGAGGATTGAAGAGTTTTTCGCAGCGAATTCCTGTAAGCTTAGATTCTTCATGACTTGGATTTCGTCGTTGGATTCGTTTAGTGATAGAGAGTTATGGGCTATACAGAGTATATTCAAAGTTCATGACAATGAAAATCCCTGTTTGATTATCGTCTCAAATTCCCTCGATTCAGCAAAAGGAAAGCAAATTCTTAGCCCATTTTCGGAAATGGGATTCTCCTTACTGGCAATATCGCCGGATTTCGATGCCATTTTCAAAAACACGGAAGCAGAGCTGTGGTTCAATCAACTCCAACAAGGAATTGTAAAAGCTGGTGAAATTTCTTTGGGTCAAAATCTCTCAAATTTACTCCGATTAACTCTGTTATATAAATTCGGAGGCATTTACATCGACACCGACGTGATAATCCTACAGAACTTCACAAATCTTCGAAACGCAATTGGGGCTCAAACCATGGATTTGAAAACAGGAAATTGGAGCAGATTGAACAACGCAGTGATGATATTCGACAAGAATCACCCACTTCTACTTCAATTTATCAAAGAATTTGCCACAACATTCGACGGAAATAAATGGGGACACAACGGACCTTATTTAGTATCGAGAGTAATATCAAGATTGAATCAAAATAGTGAGTTTAACTTGACTATTCTTCCTCCATCGGCCTTTTACCCGGTTGTTTGGAACAGAATCAAAACTTTTTTTCAAGGTCCAAAAGATGCAGTTCACCTGAAATGGATAATTGCAAAATTGAGACACATTCAAACAAAAAGTTTGGCTCTACATTTGTGGAACAACCATAGTCGAAAGCTTCAAGTTGAGAAGGGAAGCATCGTTGATATTATAGTAAATATGAAATTATGAAAGTATACTATAGAATTCAAAAGCTTTTAAGTTCAATTTTGTGAAATTTGTTTATGATATTATACAACAAATTTTA

mRNA sequence

AATGATTGGTAATTTCAACCCTCTCAAGGAATTTACTTTCCATTTTCTCTCATCCCTTCCCACTTCTTTTCTTTCTCTTCTCCTTCTCTTCCTCTTGGCTTACAACGGCCTTCATGTTTTCTCTATTTCCCCACCCTCACCGGAGAAGACAATTCCTCATCCCGCTCCTTTCTCGCCCCAAAAACGACCCACTGCCGATTCCACTTCAACCCATCTTCTGTTTTCCATTCATGAAAACCATCCACCACCGGTCTTAAAATCAAATACCTCTGTTTTTCATCATCCCCACCTCAAATCCCGGCCCGCAAAGAGAGTCGGGAAGCATAAGCGCCGCCTGCGAAGTCTCCGGTCGGAATTGAAAGAAAGCGACTTTTCAGCGAGGATTGAAGAGTTTTTCGCAGCGAATTCCTGTAAGCTTAGATTCTTCATGACTTGGATTTCGTCGTTGGATTCGTTTAGTGATAGAGAGTTATGGGCTATACAGAGTATATTCAAAGTTCATGACAATGAAAATCCCTGTTTGATTATCGTCTCAAATTCCCTCGATTCAGCAAAAGGAAAGCAAATTCTTAGCCCATTTTCGGAAATGGGATTCTCCTTACTGGCAATATCGCCGGATTTCGATGCCATTTTCAAAAACACGGAAGCAGAGCTGTGGTTCAATCAACTCCAACAAGGAATTGTAAAAGCTGGTGAAATTTCTTTGGGTCAAAATCTCTCAAATTTACTCCGATTAACTCTGTTATATAAATTCGGAGGCATTTACATCGACACCGACGTGATAATCCTACAGAACTTCACAAATCTTCGAAACGCAATTGGGGCTCAAACCATGGATTTGAAAACAGGAAATTGGAGCAGATTGAACAACGCAGTGATGATATTCGACAAGAATCACCCACTTCTACTTCAATTTATCAAAGAATTTGCCACAACATTCGACGGAAATAAATGGGGACACAACGGACCTTATTTAGTATCGAGAGTAATATCAAGATTGAATCAAAATAGTGAGTTTAACTTGACTATTCTTCCTCCATCGGCCTTTTACCCGGTTGTTTGGAACAGAATCAAAACTTTTTTTCAAGGTCCAAAAGATGCAGTTCACCTGAAATGGATAATTGCAAAATTGAGACACATTCAAACAAAAAGTTTGGCTCTACATTTGTGGAACAACCATAGTCGAAAGCTTCAAGTTGAGAAGGGAAGCATCGTTGATATTATAGTAAATATGAAATTATGAAAGTATACTATAGAATTCAAAAGCTTTTAAGTTCAATTTTGTGAAATTTGTTTATGATATTATACAACAAATTTTA

Coding sequence (CDS)

ATGATTGGTAATTTCAACCCTCTCAAGGAATTTACTTTCCATTTTCTCTCATCCCTTCCCACTTCTTTTCTTTCTCTTCTCCTTCTCTTCCTCTTGGCTTACAACGGCCTTCATGTTTTCTCTATTTCCCCACCCTCACCGGAGAAGACAATTCCTCATCCCGCTCCTTTCTCGCCCCAAAAACGACCCACTGCCGATTCCACTTCAACCCATCTTCTGTTTTCCATTCATGAAAACCATCCACCACCGGTCTTAAAATCAAATACCTCTGTTTTTCATCATCCCCACCTCAAATCCCGGCCCGCAAAGAGAGTCGGGAAGCATAAGCGCCGCCTGCGAAGTCTCCGGTCGGAATTGAAAGAAAGCGACTTTTCAGCGAGGATTGAAGAGTTTTTCGCAGCGAATTCCTGTAAGCTTAGATTCTTCATGACTTGGATTTCGTCGTTGGATTCGTTTAGTGATAGAGAGTTATGGGCTATACAGAGTATATTCAAAGTTCATGACAATGAAAATCCCTGTTTGATTATCGTCTCAAATTCCCTCGATTCAGCAAAAGGAAAGCAAATTCTTAGCCCATTTTCGGAAATGGGATTCTCCTTACTGGCAATATCGCCGGATTTCGATGCCATTTTCAAAAACACGGAAGCAGAGCTGTGGTTCAATCAACTCCAACAAGGAATTGTAAAAGCTGGTGAAATTTCTTTGGGTCAAAATCTCTCAAATTTACTCCGATTAACTCTGTTATATAAATTCGGAGGCATTTACATCGACACCGACGTGATAATCCTACAGAACTTCACAAATCTTCGAAACGCAATTGGGGCTCAAACCATGGATTTGAAAACAGGAAATTGGAGCAGATTGAACAACGCAGTGATGATATTCGACAAGAATCACCCACTTCTACTTCAATTTATCAAAGAATTTGCCACAACATTCGACGGAAATAAATGGGGACACAACGGACCTTATTTAGTATCGAGAGTAATATCAAGATTGAATCAAAATAGTGAGTTTAACTTGACTATTCTTCCTCCATCGGCCTTTTACCCGGTTGTTTGGAACAGAATCAAAACTTTTTTTCAAGGTCCAAAAGATGCAGTTCACCTGAAATGGATAATTGCAAAATTGAGACACATTCAAACAAAAAGTTTGGCTCTACATTTGTGGAACAACCATAGTCGAAAGCTTCAAGTTGAGAAGGGAAGCATCGTTGATATTATAGTAAATATGAAATTATGA

Protein sequence

MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQKRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMKL*
Homology
BLAST of CsGy1G011900 vs. ExPASy Swiss-Prot
Match: P0C8Q4 (Uncharacterized protein At4g19900 OS=Arabidopsis thaliana OX=3702 GN=At4g19900 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 5.1e-27
Identity = 86/291 (29.55%), Postives = 138/291 (47.42%), Query Frame = 0

Query: 124 FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDS 183
           FS  ++ FF    C +R FM W S    FS R    ++S+   H   + C+++ S +++ 
Sbjct: 355 FSDFMDSFFRKEKCSMRVFMVWNSPGWMFSVRHQRGLESLLSQH--RDACVVVFSETVEL 414

Query: 184 AKGKQILSPFSEMGFSLLAISPDFDAIFKNTE----AELWFNQLQQGIVKAGEISLGQNL 243
                  + F +  + +    P+ D + ++T     A +WF+  +             + 
Sbjct: 415 ---DFFRNSFVKDSYKVAVAMPNLDELLQDTPTHVFASVWFDWRKTKFYPT-------HY 474

Query: 244 SNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNH 303
           S L+RL  LYK+GG+Y+D+DVI+L + ++LRN IG +  D   G    LN AVM F+K  
Sbjct: 475 SELVRLAALYKYGGVYLDSDVIVLGSLSSLRNTIGME--DQVAG--ESLNGAVMSFEKKS 534

Query: 304 PLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRL--NQNSEFN---LTILPPSAFYPVVW 363
           P LL+ + E+  T+D      NG  L++RV  R    +N   N   L I P S F+P+  
Sbjct: 535 PFLLECLNEYYLTYDDKCLRCNGADLLTRVAKRFLNGKNRRMNQQELNIRPSSVFFPINS 594

Query: 364 NRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIV 406
            +I  +F  P             + I  +SL  H WN+ +  L  E  S+V
Sbjct: 595 QQITNYFAYPAIEDERSQQDESFKKILNESLTFHFWNSVTSSLIPEPESLV 629

BLAST of CsGy1G011900 vs. ExPASy Swiss-Prot
Match: Q9JI93 (Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=A4galt PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.4e-24
Identity = 71/222 (31.98%), Postives = 108/222 (48.65%), Query Frame = 0

Query: 196 MGFSLLAISP-------DFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLL 255
           +G SLL+  P       D   +F++T    W+ + Q       E  L   LS+  R+ LL
Sbjct: 135 LGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQH----RWEPYLLPVLSDASRIALL 194

Query: 256 YKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKE 315
           +KFGGIY+DTD I+L+N  NL N +G Q+  +       LN A + F++ H  L   I++
Sbjct: 195 WKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYV-------LNGAFLAFERKHEFLALCIRD 254

Query: 316 FATTFDGNKWGHNGPYLVSRVI-------SRLNQNSEFNLTILPPSAFYPVVWNRIKTFF 375
           F   ++G  WGH GP L++RV        S     +   +T LPP AFYP+ W   K +F
Sbjct: 255 FVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYF 314

Query: 376 QGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGS 404
           +             +L  +   + A+H+WN  S+   +E  S
Sbjct: 315 EDVSP--------EELAQLLNATYAVHVWNKKSQGTHLEATS 337

BLAST of CsGy1G011900 vs. ExPASy Swiss-Prot
Match: Q67BJ4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4galt PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.2e-23
Identity = 67/222 (30.18%), Postives = 108/222 (48.65%), Query Frame = 0

Query: 196 MGFSLLAISP-------DFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLL 255
           +G SLL+  P       D   +F++T    W+++ +       E      LS+  R+ LL
Sbjct: 134 LGISLLSCFPNVWIRPLDLQELFEDTPLAAWYSEARH----RWEPYQLPVLSDASRIALL 193

Query: 256 YKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKE 315
           +KFGGIY+DTD I+L+N  NL N +G Q+  +       LN A + F++ H  L   + +
Sbjct: 194 WKFGGIYLDTDFIVLKNLLNLTNTLGIQSRYV-------LNGAFLAFERKHEFLALCLHD 253

Query: 316 FATTFDGNKWGHNGPYLVSRVISRL-------NQNSEFNLTILPPSAFYPVVWNRIKTFF 375
           F   ++G  WGH GP L++RV  +          ++   +T LPP AFYP+ W   K +F
Sbjct: 254 FVANYNGWIWGHQGPQLLTRVFKKWCSIQSLEKSHACRGVTALPPEAFYPIPWQNWKKYF 313

Query: 376 QGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGS 404
           +             +L  +   + A+H+WN  S+   +E  S
Sbjct: 314 EDISP--------EELTQLLNATYAVHVWNKKSQGTHLEATS 336

BLAST of CsGy1G011900 vs. ExPASy Swiss-Prot
Match: Q9N291 (Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 4.5e-23
Identity = 69/222 (31.08%), Postives = 109/222 (49.10%), Query Frame = 0

Query: 196 MGFSLLAISP-------DFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLL 255
           +G SLL+  P       D   +F++T    W+  +Q       E  L   LS+  R+ L+
Sbjct: 128 LGISLLSCFPNVQMLPLDLRELFQDTPLADWYAAVQ----GRWEPYLLPVLSDASRIALM 187

Query: 256 YKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKE 315
           +KFGGIY+DTD I+L+N  NL N +G Q+  +       LN A + F++ H  +   +++
Sbjct: 188 WKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYV-------LNGAFLAFERRHEFMALCMRD 247

Query: 316 FATTFDGNKWGHNGPYLVSRVI-------SRLNQNSEFNLTILPPSAFYPVVWNRIKTFF 375
           F   ++G  WGH GP L++RV        S     S   +T LPP AFYP+ W   K +F
Sbjct: 248 FVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRSCRGVTTLPPEAFYPIPWQDWKKYF 307

Query: 376 QGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGS 404
           +             +L  + + + A+H+WN  S+  + E  S
Sbjct: 308 EDINP--------EELPRLLSATYAVHVWNKKSQGTRFEATS 330

BLAST of CsGy1G011900 vs. ExPASy Swiss-Prot
Match: Q9NPC4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 7.6e-23
Identity = 68/222 (30.63%), Postives = 109/222 (49.10%), Query Frame = 0

Query: 196 MGFSLLAISP-------DFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLL 255
           +G SLL+  P       D   +F++T    W+  +Q       E  L   LS+  R+ L+
Sbjct: 128 LGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQ----GRWEPYLLPVLSDASRIALM 187

Query: 256 YKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKE 315
           +KFGGIY+DTD I+L+N  NL N +G Q+  +       LN A + F++ H  +   +++
Sbjct: 188 WKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYV-------LNGAFLAFERRHEFMALCMRD 247

Query: 316 FATTFDGNKWGHNGPYLVSRVI-------SRLNQNSEFNLTILPPSAFYPVVWNRIKTFF 375
           F   ++G  WGH GP L++RV        S     +   +T LPP AFYP+ W   K +F
Sbjct: 248 FVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYF 307

Query: 376 QGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGS 404
           +             +L  + + + A+H+WN  S+  + E  S
Sbjct: 308 EDINP--------EELPRLLSATYAVHVWNKKSQGTRFEATS 330

BLAST of CsGy1G011900 vs. NCBI nr
Match: XP_008442286.1 (PREDICTED: lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo] >KAA0056972.1 lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo var. makuwa] >TYK26398.1 lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 763 bits (1969), Expect = 7.09e-277
Identity = 379/412 (91.99%), Postives = 396/412 (96.12%), Query Frame = 0

Query: 1   MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ 60
           MIGNFNPLKE T H LSSLPTS LSLLLLFLLAYNGLHVFSISPPSPEKTI HPAPFSPQ
Sbjct: 1   MIGNFNPLKESTLHLLSSLPTSLLSLLLLFLLAYNGLHVFSISPPSPEKTISHPAPFSPQ 60

Query: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120
           KRPTADSTSTHLLFSIHENHPPPVLKSN+SVFHHPHLKSRPAKRVGKHKRRLRSLRSELK
Sbjct: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNSSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120

Query: 121 ESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNS 180
           E++FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVH+N NPCLIIVSNS
Sbjct: 121 ENEFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHENGNPCLIIVSNS 180

Query: 181 LDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLS 240
           LDS KGKQ+LSPFSEMGFSLLAISPDFD+IFKNT+AE WFNQL+QGI+K GEISLGQNLS
Sbjct: 181 LDSTKGKQVLSPFSEMGFSLLAISPDFDSIFKNTDAESWFNQLRQGIIKPGEISLGQNLS 240

Query: 241 NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300
           NLLRLTLLYKFGGIYID DVIIL++FTNLRN IGAQTMDLKTGNWSRLNNAVMIFDKNHP
Sbjct: 241 NLLRLTLLYKFGGIYIDADVIILKSFTNLRNVIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300

Query: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF 360
           LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQN  FNLTILPPSAFYPVVWN+IKT 
Sbjct: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNPGFNLTILPPSAFYPVVWNKIKTL 360

Query: 361 FQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMK 412
           FQGPKDAVHLKW+IAKLR IQ+KSLALHLWN+HSRKL+VEKGSIVDII+ MK
Sbjct: 361 FQGPKDAVHLKWVIAKLRQIQSKSLALHLWNSHSRKLEVEKGSIVDIIIKMK 412

BLAST of CsGy1G011900 vs. NCBI nr
Match: XP_031738021.1 (lactosylceramide 4-alpha-galactosyltransferase [Cucumis sativus])

HSP 1 Score: 700 bits (1806), Expect = 8.30e-253
Identity = 363/413 (87.89%), Postives = 364/413 (88.14%), Query Frame = 0

Query: 1   MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ 60
           MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ
Sbjct: 1   MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ 60

Query: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120
           KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK
Sbjct: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120

Query: 121 ESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNS 180
           ESDFSARIEEFFAANS                                            
Sbjct: 121 ESDFSARIEEFFAANS-------------------------------------------- 180

Query: 181 LDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLS 240
              +KGKQILSPFSEMGFSLLAISPDFD IFKNTEAELWFNQLQQGIVKAGEISLGQNLS
Sbjct: 181 ---SKGKQILSPFSEMGFSLLAISPDFDVIFKNTEAELWFNQLQQGIVKAGEISLGQNLS 240

Query: 241 NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300
           NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP
Sbjct: 241 NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300

Query: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF 360
           LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF
Sbjct: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF 360

Query: 361 FQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMKL 413
           FQGPKD VHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMKL
Sbjct: 361 FQGPKDGVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMKL 366

BLAST of CsGy1G011900 vs. NCBI nr
Match: XP_038905948.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Benincasa hispida])

HSP 1 Score: 671 bits (1730), Expect = 3.39e-240
Identity = 344/430 (80.00%), Postives = 376/430 (87.44%), Query Frame = 0

Query: 1   MIGNFN------PLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHP 60
           MIGN         LKE TF FLSSLPTS LSLLLL LLAYNGLHVF  SPP  EKT PHP
Sbjct: 1   MIGNVKHSHPLLTLKESTFRFLSSLPTSLLSLLLLVLLAYNGLHVFCCSPPLSEKT-PHP 60

Query: 61  APFSPQKR----PTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLK--------SRPA 120
           A FSP+KR     ++ STSTH+LFS+ ENHPPP LKSN+S+FHHPH K        SR A
Sbjct: 61  AAFSPEKRWSNLSSSVSTSTHVLFSVEENHPPPTLKSNSSIFHHPHFKTRRGDDSASRLA 120

Query: 121 KRVGKHKRRLRSLRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQS 180
           KRVGKHKRRLRSLR E +E++FSARIEEFFAANSCKLRFFMTWISSL+SFSDRELWAIQS
Sbjct: 121 KRVGKHKRRLRSLRPESRENEFSARIEEFFAANSCKLRFFMTWISSLNSFSDRELWAIQS 180

Query: 181 IFKVHDNENPCLIIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQ 240
           +FKVH++ NPCL+IVSNSLDS KGKQILSPFSE GFSL AISPDFD+IF+NTEAE WFN 
Sbjct: 181 VFKVHEHGNPCLVIVSNSLDSTKGKQILSPFSEKGFSLFAISPDFDSIFRNTEAEPWFNL 240

Query: 241 LQQGIVKAGEISLGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKT 300
           L+QGI+K GEISLGQNLSNLLRL LLYKFGGIYID DVIIL+NFTNLRN IGAQT++LKT
Sbjct: 241 LRQGIIKPGEISLGQNLSNLLRLALLYKFGGIYIDADVIILKNFTNLRNVIGAQTINLKT 300

Query: 301 GNWSRLNNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLT 360
           GNWSRLNNAVMIFDKNHPLLLQFI+EFATTFDGNKWGHNGPYLVSRVISRLNQN  FNLT
Sbjct: 301 GNWSRLNNAVMIFDKNHPLLLQFIQEFATTFDGNKWGHNGPYLVSRVISRLNQNPGFNLT 360

Query: 361 ILPPSAFYPVVWNRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKG 412
           +LPPSAFYPVVWNRIK+ FQGPKDAVHLKW+IAKL++I++ SLALHLWN+HSRKLQVEKG
Sbjct: 361 VLPPSAFYPVVWNRIKSLFQGPKDAVHLKWVIAKLKYIRSTSLALHLWNSHSRKLQVEKG 420

BLAST of CsGy1G011900 vs. NCBI nr
Match: KAG7019628.1 (hypothetical protein SDJN02_18591, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639 bits (1647), Expect = 1.96e-227
Identity = 326/418 (77.99%), Postives = 359/418 (85.89%), Query Frame = 0

Query: 7   PLKEFTF-HFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQKRPTA 66
           PLKE  + HFLSSL  S LSLLLLFLLA+NGLHVF    PSPEKTIPH  PFSP KR + 
Sbjct: 20  PLKESAYYHFLSSLTASLLSLLLLFLLAFNGLHVFFTYSPSPEKTIPHSPPFSPDKRCSD 79

Query: 67  DSTS----THLLFSIHENHPPPVLKSNTSVFHHPHLKSRP-------AKRVGKHKRRLRS 126
            S+S    TH+LFSI E HPP + KSN+SVFH+PH+K R        AKRVGKHKRRLRS
Sbjct: 80  LSSSVPASTHVLFSIEEKHPPVIFKSNSSVFHNPHVKIRADDSAEMAAKRVGKHKRRLRS 139

Query: 127 LRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCL 186
           LR E +E++FSARIEEF  ANSCKLRFFM WIS L+SFSDRE WAIQSIFK H+N NPCL
Sbjct: 140 LRLESRENEFSARIEEFLVANSCKLRFFMIWISPLNSFSDREFWAIQSIFKAHENGNPCL 199

Query: 187 IIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEIS 246
           IIVSNSLDSAKGKQILSPFSE GF L+AISPDFD+IF+NTEAE WFNQL+QGI+K GEIS
Sbjct: 200 IIVSNSLDSAKGKQILSPFSEKGFPLIAISPDFDSIFRNTEAEPWFNQLRQGIIKPGEIS 259

Query: 247 LGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMI 306
           L QNLSNLLRL LLYKFGGIY+D DVIIL+NFTNL+N IGAQT+DLKTGNWSRLNNAVMI
Sbjct: 260 LAQNLSNLLRLALLYKFGGIYLDADVIILKNFTNLQNVIGAQTIDLKTGNWSRLNNAVMI 319

Query: 307 FDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVW 366
           FDKNHPLLLQFI+EFATTFDGNKWGHNGPYLVSRV+SRLNQN  FNLT+LPPSAFYPVVW
Sbjct: 320 FDKNHPLLLQFIQEFATTFDGNKWGHNGPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVW 379

Query: 367 NRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMK 412
           +RI+  FQ PKDA HLKW+ AKL+HI++ SLALHLWN+HSRKLQVEKGSIVDIIV MK
Sbjct: 380 SRIRALFQSPKDAAHLKWVKAKLKHIESHSLALHLWNSHSRKLQVEKGSIVDIIVTMK 437

BLAST of CsGy1G011900 vs. NCBI nr
Match: XP_023001721.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita maxima])

HSP 1 Score: 635 bits (1638), Expect = 4.58e-226
Identity = 322/424 (75.94%), Postives = 360/424 (84.91%), Query Frame = 0

Query: 1   MIGNFNPLKEFTF-HFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSP 60
           ++    PLKE  + HFLSSL  SFLSLLLLFLLA+NGLHVF    PSPEKTIPH  PFSP
Sbjct: 14  LLRRLQPLKESAYYHFLSSLTASFLSLLLLFLLAFNGLHVFFTYSPSPEKTIPHSPPFSP 73

Query: 61  QKRPTADSTS----THLLFSIHENHPPPVLKSNTSVFHHPHLKSRP-------AKRVGKH 120
            KR +  S+S    TH+LFSI + HPP + KSN+SVFH+PH+K R        AKRVGK+
Sbjct: 74  DKRCSNLSSSVPASTHVLFSIEQKHPPVIFKSNSSVFHNPHVKIRADDSAELAAKRVGKY 133

Query: 121 KRRLRSLRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHD 180
           KRRLRSLR E +E++FSARIEEF AANSCKLRFFM WIS L+SFSDRE WAIQSIFK H+
Sbjct: 134 KRRLRSLRLESRENEFSARIEEFLAANSCKLRFFMIWISPLNSFSDREFWAIQSIFKAHE 193

Query: 181 NENPCLIIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIV 240
           N NPCL+IVSNSLDS KGKQILSPFSE GF L+ ISPDFD+IF+NTEAE WFNQL+QGI+
Sbjct: 194 NGNPCLVIVSNSLDSTKGKQILSPFSEKGFPLIVISPDFDSIFRNTEAEPWFNQLRQGII 253

Query: 241 KAGEISLGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRL 300
           K GEISL QNLSNLLRL LLY FGGIY+D DVIIL+NFTNLRN IGAQT+DLKTGNWSRL
Sbjct: 254 KPGEISLAQNLSNLLRLALLYNFGGIYLDADVIILKNFTNLRNVIGAQTIDLKTGNWSRL 313

Query: 301 NNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSA 360
           NNAVMIFDKNHPLLLQFI+EF TTFDGNKWGHNGPYLVSRV+SRLNQN  FNLT+LPPSA
Sbjct: 314 NNAVMIFDKNHPLLLQFIQEFTTTFDGNKWGHNGPYLVSRVVSRLNQNPGFNLTVLPPSA 373

Query: 361 FYPVVWNRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDII 412
           FYPVVW+RI+  FQ PKDAVHLKW+ AKL+HI+++SLALHLWN+HSRK QVEKGSIVDII
Sbjct: 374 FYPVVWSRIRALFQSPKDAVHLKWVKAKLKHIESQSLALHLWNSHSRKFQVEKGSIVDII 433

BLAST of CsGy1G011900 vs. ExPASy TrEMBL
Match: A0A5D3DSB7 (Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00610 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 3.43e-277
Identity = 379/412 (91.99%), Postives = 396/412 (96.12%), Query Frame = 0

Query: 1   MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ 60
           MIGNFNPLKE T H LSSLPTS LSLLLLFLLAYNGLHVFSISPPSPEKTI HPAPFSPQ
Sbjct: 1   MIGNFNPLKESTLHLLSSLPTSLLSLLLLFLLAYNGLHVFSISPPSPEKTISHPAPFSPQ 60

Query: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120
           KRPTADSTSTHLLFSIHENHPPPVLKSN+SVFHHPHLKSRPAKRVGKHKRRLRSLRSELK
Sbjct: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNSSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120

Query: 121 ESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNS 180
           E++FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVH+N NPCLIIVSNS
Sbjct: 121 ENEFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHENGNPCLIIVSNS 180

Query: 181 LDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLS 240
           LDS KGKQ+LSPFSEMGFSLLAISPDFD+IFKNT+AE WFNQL+QGI+K GEISLGQNLS
Sbjct: 181 LDSTKGKQVLSPFSEMGFSLLAISPDFDSIFKNTDAESWFNQLRQGIIKPGEISLGQNLS 240

Query: 241 NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300
           NLLRLTLLYKFGGIYID DVIIL++FTNLRN IGAQTMDLKTGNWSRLNNAVMIFDKNHP
Sbjct: 241 NLLRLTLLYKFGGIYIDADVIILKSFTNLRNVIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300

Query: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF 360
           LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQN  FNLTILPPSAFYPVVWN+IKT 
Sbjct: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNPGFNLTILPPSAFYPVVWNKIKTL 360

Query: 361 FQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMK 412
           FQGPKDAVHLKW+IAKLR IQ+KSLALHLWN+HSRKL+VEKGSIVDII+ MK
Sbjct: 361 FQGPKDAVHLKWVIAKLRQIQSKSLALHLWNSHSRKLEVEKGSIVDIIIKMK 412

BLAST of CsGy1G011900 vs. ExPASy TrEMBL
Match: A0A1S3B637 (lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103486194 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 3.43e-277
Identity = 379/412 (91.99%), Postives = 396/412 (96.12%), Query Frame = 0

Query: 1   MIGNFNPLKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQ 60
           MIGNFNPLKE T H LSSLPTS LSLLLLFLLAYNGLHVFSISPPSPEKTI HPAPFSPQ
Sbjct: 1   MIGNFNPLKESTLHLLSSLPTSLLSLLLLFLLAYNGLHVFSISPPSPEKTISHPAPFSPQ 60

Query: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120
           KRPTADSTSTHLLFSIHENHPPPVLKSN+SVFHHPHLKSRPAKRVGKHKRRLRSLRSELK
Sbjct: 61  KRPTADSTSTHLLFSIHENHPPPVLKSNSSVFHHPHLKSRPAKRVGKHKRRLRSLRSELK 120

Query: 121 ESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNS 180
           E++FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVH+N NPCLIIVSNS
Sbjct: 121 ENEFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHENGNPCLIIVSNS 180

Query: 181 LDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLS 240
           LDS KGKQ+LSPFSEMGFSLLAISPDFD+IFKNT+AE WFNQL+QGI+K GEISLGQNLS
Sbjct: 181 LDSTKGKQVLSPFSEMGFSLLAISPDFDSIFKNTDAESWFNQLRQGIIKPGEISLGQNLS 240

Query: 241 NLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300
           NLLRLTLLYKFGGIYID DVIIL++FTNLRN IGAQTMDLKTGNWSRLNNAVMIFDKNHP
Sbjct: 241 NLLRLTLLYKFGGIYIDADVIILKSFTNLRNVIGAQTMDLKTGNWSRLNNAVMIFDKNHP 300

Query: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTF 360
           LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQN  FNLTILPPSAFYPVVWN+IKT 
Sbjct: 301 LLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNPGFNLTILPPSAFYPVVWNKIKTL 360

Query: 361 FQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMK 412
           FQGPKDAVHLKW+IAKLR IQ+KSLALHLWN+HSRKL+VEKGSIVDII+ MK
Sbjct: 361 FQGPKDAVHLKWVIAKLRQIQSKSLALHLWNSHSRKLEVEKGSIVDIIIKMK 412

BLAST of CsGy1G011900 vs. ExPASy TrEMBL
Match: A0A6J1KJE1 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111495773 PE=4 SV=1)

HSP 1 Score: 635 bits (1638), Expect = 2.22e-226
Identity = 322/424 (75.94%), Postives = 360/424 (84.91%), Query Frame = 0

Query: 1   MIGNFNPLKEFTF-HFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSP 60
           ++    PLKE  + HFLSSL  SFLSLLLLFLLA+NGLHVF    PSPEKTIPH  PFSP
Sbjct: 14  LLRRLQPLKESAYYHFLSSLTASFLSLLLLFLLAFNGLHVFFTYSPSPEKTIPHSPPFSP 73

Query: 61  QKRPTADSTS----THLLFSIHENHPPPVLKSNTSVFHHPHLKSRP-------AKRVGKH 120
            KR +  S+S    TH+LFSI + HPP + KSN+SVFH+PH+K R        AKRVGK+
Sbjct: 74  DKRCSNLSSSVPASTHVLFSIEQKHPPVIFKSNSSVFHNPHVKIRADDSAELAAKRVGKY 133

Query: 121 KRRLRSLRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHD 180
           KRRLRSLR E +E++FSARIEEF AANSCKLRFFM WIS L+SFSDRE WAIQSIFK H+
Sbjct: 134 KRRLRSLRLESRENEFSARIEEFLAANSCKLRFFMIWISPLNSFSDREFWAIQSIFKAHE 193

Query: 181 NENPCLIIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIV 240
           N NPCL+IVSNSLDS KGKQILSPFSE GF L+ ISPDFD+IF+NTEAE WFNQL+QGI+
Sbjct: 194 NGNPCLVIVSNSLDSTKGKQILSPFSEKGFPLIVISPDFDSIFRNTEAEPWFNQLRQGII 253

Query: 241 KAGEISLGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRL 300
           K GEISL QNLSNLLRL LLY FGGIY+D DVIIL+NFTNLRN IGAQT+DLKTGNWSRL
Sbjct: 254 KPGEISLAQNLSNLLRLALLYNFGGIYLDADVIILKNFTNLRNVIGAQTIDLKTGNWSRL 313

Query: 301 NNAVMIFDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSA 360
           NNAVMIFDKNHPLLLQFI+EF TTFDGNKWGHNGPYLVSRV+SRLNQN  FNLT+LPPSA
Sbjct: 314 NNAVMIFDKNHPLLLQFIQEFTTTFDGNKWGHNGPYLVSRVVSRLNQNPGFNLTVLPPSA 373

Query: 361 FYPVVWNRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDII 412
           FYPVVW+RI+  FQ PKDAVHLKW+ AKL+HI+++SLALHLWN+HSRK QVEKGSIVDII
Sbjct: 374 FYPVVWSRIRALFQSPKDAVHLKWVKAKLKHIESQSLALHLWNSHSRKFQVEKGSIVDII 433

BLAST of CsGy1G011900 vs. ExPASy TrEMBL
Match: A0A6J1EH01 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111434013 PE=4 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 2.78e-219
Identity = 314/414 (75.85%), Postives = 350/414 (84.54%), Query Frame = 0

Query: 7   PLKEFTF-HFLSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEKTIPHPAPFSPQKRPTA 66
           PLKE  + HFLSSL  S LSLLLLFLLA+NGLHVF        KTIPH +PFSP KR   
Sbjct: 20  PLKESAYYHFLSSLTASLLSLLLLFLLAFNGLHVFL-------KTIPHSSPFSPDKR--C 79

Query: 67  DSTSTHLLFSIHENHPPPVLKSNTSVFHHPHLK-------SRPAKRVGKHKRRLRSLRSE 126
            + S+ +LFSI E HPP + KSN+SVFH+PH+K          AKRVGKHKRRLRSLR E
Sbjct: 80  SNLSSSVLFSIEEKHPPVIFKSNSSVFHNPHVKICADDSAELAAKRVGKHKRRLRSLRLE 139

Query: 127 LKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVS 186
            +E++FSARIEEF AANSCKLRFFM WIS L+SFSDRE WAIQSIFK H+N NPCL IVS
Sbjct: 140 SRENEFSARIEEFLAANSCKLRFFMIWISPLNSFSDREFWAIQSIFKAHENGNPCLTIVS 199

Query: 187 NSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQN 246
           NSLDS KGKQILSPFS+ GF L+AISPDFD+IF+NT+AE WFNQL+QG +K GEISL QN
Sbjct: 200 NSLDSTKGKQILSPFSKKGFPLIAISPDFDSIFRNTQAEPWFNQLRQGTIKPGEISLAQN 259

Query: 247 LSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKN 306
           LSNLLRL LLYKFGGIY+D DVIIL+NFTNLRN IGAQT+DLKTGNWSRLNNAVMIFDKN
Sbjct: 260 LSNLLRLALLYKFGGIYLDADVIILKNFTNLRNVIGAQTIDLKTGNWSRLNNAVMIFDKN 319

Query: 307 HPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIK 366
           HPLLLQFI+EFATTFDGNKWGHNGPYLVSRV+SRLNQN  FNLT+LPPSAFYPVVW+RI+
Sbjct: 320 HPLLLQFIQEFATTFDGNKWGHNGPYLVSRVVSRLNQNPGFNLTVLPPSAFYPVVWSRIR 379

Query: 367 TFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVNMK 412
             FQ PKDA HLKW+ AKL+HI++ SLALHLWN+HSRKLQVEKGS+VDIIV MK
Sbjct: 380 ALFQSPKDAAHLKWVKAKLKHIESHSLALHLWNSHSRKLQVEKGSVVDIIVTMK 424

BLAST of CsGy1G011900 vs. ExPASy TrEMBL
Match: A0A6J1CG17 (uncharacterized protein At4g19900 OS=Momordica charantia OX=3673 GN=LOC111010894 PE=4 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 2.33e-171
Identity = 231/294 (78.57%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 115 LRSELKESDFSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCL 174
           LR E +E++FSARIEEFF AN CKLRFFMTWIS L SFSDRELWAIQS+FK H N N CL
Sbjct: 1   LRPESRENEFSARIEEFFGANLCKLRFFMTWISPLSSFSDRELWAIQSLFKAHGNGNSCL 60

Query: 175 IIVSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEIS 234
           +IVSNSLDS +G+QILS FSE GF ++AISPDFD+IF++TEAE WFNQL++GI+K GEI+
Sbjct: 61  VIVSNSLDSTEGRQILSQFSENGFRVIAISPDFDSIFRDTEAEAWFNQLRRGIIKPGEIA 120

Query: 235 LGQNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMI 294
           +GQNLSNLLRL LLYKFGGIYID DVIIL++F NLRNAIGAQT+DL+TGNWSRLNNAVMI
Sbjct: 121 IGQNLSNLLRLALLYKFGGIYIDADVIILKSFLNLRNAIGAQTVDLRTGNWSRLNNAVMI 180

Query: 295 FDKNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVW 354
           FDKNHPLLL+FIKEFA+TFDGN+WGHNGPYLVSRV+SRLNQN  FN ++LPPSAFYPVVW
Sbjct: 181 FDKNHPLLLEFIKEFASTFDGNRWGHNGPYLVSRVVSRLNQNPGFNFSVLPPSAFYPVVW 240

Query: 355 NRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDII 408
           +R++  FQGPKDAVH KW+IAKLR I++KSLALHLWN+HSRKL+VEKGSI+D+I
Sbjct: 241 SRVRGLFQGPKDAVHSKWVIAKLRQIRSKSLALHLWNSHSRKLEVEKGSIIDLI 294

BLAST of CsGy1G011900 vs. TAIR 10
Match: AT1G61050.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.4e-96
Identity = 195/411 (47.45%), Postives = 262/411 (63.75%), Query Frame = 0

Query: 8   LKEFTFHFLSSLPTSFLSLLLLFLLAYNGLHVFSIS--PPSPEKTIPHPAPFSPQKRPTA 67
           LK     F+  LP S L LLL+ LL YN   VFS+   P  P ++   P         T+
Sbjct: 16  LKRLIVSFVFCLPMSLLGLLLMLLLIYNSFSVFSLHLVPNQPIQSTLSPTHLQILHHQTS 75

Query: 68  DSTS---THLLFSIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRRLR-----SLRS 127
            S+S   + LL  + E     + K N S        +R  K+  + KR        + R 
Sbjct: 76  TSSSVSDSSLLLVVKETSLGFIQKQNVS-------STRIEKKTRRFKRSTELTPAITQRL 135

Query: 128 ELKESD-FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLII 187
           ++K    F  R++   + +SC+  FFMTWISS++SF DRE + I+S+FK H   N CLI+
Sbjct: 136 QVKSRQRFQTRVKSLLSKSSCESLFFMTWISSIESFGDRERFTIESLFKFH--PNGCLIL 195

Query: 188 VSNSLDSAKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLG 247
           VSNS D  +G  IL PF++ G  +L I PDF  IFK+T AE WF +L++G +  G I L 
Sbjct: 196 VSNSFDCDRGTLILKPFTDKGLKVLPIKPDFAYIFKDTSAEKWFERLKKGTLSPGVIPLE 255

Query: 248 QNLSNLLRLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFD 307
           QNLSNLLRL LLYK+GGIY+DTDVIIL++ +NL N IGAQT+D  T  WSRLNNAV+IFD
Sbjct: 256 QNLSNLLRLVLLYKYGGIYLDTDVIILKSLSNLHNVIGAQTVDPVTKKWSRLNNAVLIFD 315

Query: 308 KNHPLLLQFIKEFATTFDGNKWGHNGPYLVSRVISR--LNQNSEFNLTILPPSAFYPVVW 367
           KNHPLL +FI EF+ TF+GNKWGHNGPYLVSRVI+R  ++ +S+   ++LPPSAFYPV W
Sbjct: 316 KNHPLLKRFIDEFSRTFNGNKWGHNGPYLVSRVITRIKISSSSDLGFSVLPPSAFYPVDW 375

Query: 368 NRIKTFFQGPKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIV 406
            RIK F++ P +     W+  +L H++  + A+HLWN  S+KL++E+GSI+
Sbjct: 376 TRIKGFYRAPTNESD-AWLRKRLTHLRKNTFAVHLWNRESKKLRIEEGSII 416

BLAST of CsGy1G011900 vs. TAIR 10
Match: AT5G01250.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 305.1 bits (780), Expect = 8.8e-83
Identity = 143/280 (51.07%), Postives = 195/280 (69.64%), Query Frame = 0

Query: 124 FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDS 183
           F  R+ E F  + C++ F MTWIS  D F +RE+ AI+S+FK H     CL+I+S ++DS
Sbjct: 111 FQKRVNE-FVGDGCEVNFVMTWISPADFFGNREVLAIESVFKSH--PYGCLMILSATMDS 170

Query: 184 AKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLL 243
            +G   L PF + G+ +LA++PD   + K T  ELW ++++ G    G+ISL QNLSNL+
Sbjct: 171 PQGYATLKPFIDRGYKVLAVTPDLPFLLKGTAGELWLDEIKSGKRDPGKISLAQNLSNLM 230

Query: 244 RLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLL 303
           RL  LYK+GG+Y+DTD+I+L++F  LRN IGAQT+D  + NW+RLNNAV+IFDKNHPLLL
Sbjct: 231 RLAYLYKYGGVYLDTDMIVLKSFKGLRNVIGAQTLDPSSTNWTRLNNAVLIFDKNHPLLL 290

Query: 304 QFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTFFQG 363
           +F++EFA TF+GN WG+NGPYLVSRV   +  +S +N T++ PS FY V W  IK  F+ 
Sbjct: 291 KFMEEFAKTFNGNIWGYNGPYLVSRVARAVEGSSGYNFTVMRPSVFYSVNWLEIKKLFKV 350

Query: 364 PKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGS 404
           PK     KW+  KL H+Q     LHLWN  SRK ++E+GS
Sbjct: 351 PKTEKDSKWVKTKLLHMQRNGYGLHLWNKFSRKYEIEQGS 387

BLAST of CsGy1G011900 vs. TAIR 10
Match: AT3G09020.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 295.0 bits (754), Expect = 9.1e-80
Identity = 139/287 (48.43%), Postives = 198/287 (68.99%), Query Frame = 0

Query: 124 FSARIEEFFAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDS 183
           F  R  EF   + C+++F MTWIS  + F  RE+ +++S+FK H     CL+I+S+++DS
Sbjct: 115 FQQRATEFL-RDDCEVKFMMTWISPAELFGKREILSVESVFKSH--ARGCLMILSSTMDS 174

Query: 184 AKGKQILSPFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLL 243
            +G +IL PF + G+ ++A++PD   + K+T  E W  ++Q G    G+ISL QNLSNL+
Sbjct: 175 LQGFRILKPFLDRGYRVMAVTPDLPFLLKDTAGESWLEEIQTGKRDPGKISLAQNLSNLM 234

Query: 244 RLTLLYKFGGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLL 303
           RL  L+KFGG+Y+DTD+I+L++F  LRN IGAQT++  + NW+RLNNAV+IFDKNHP LL
Sbjct: 235 RLAYLFKFGGVYLDTDMIVLKSFKTLRNVIGAQTLEPVSRNWTRLNNAVLIFDKNHPFLL 294

Query: 304 QFIKEFATTFDGNKWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTFFQG 363
           + I+EFA TF+GN WGHNGPYLVSRV   +     +N TIL P AFYPV W  I+  F+ 
Sbjct: 295 KSIEEFALTFNGNVWGHNGPYLVSRVARAVEGTDGYNFTILTPPAFYPVNWVEIEKLFKV 354

Query: 364 PKDAVHLKWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVN 411
           P+     K +  K+  +Q +S  LHLWN  SRK ++E+GS +D +V+
Sbjct: 355 PRTEKDSKRVQVKVLEMQKRSYGLHLWNKFSRKFEIEQGSAMDKLVS 398

BLAST of CsGy1G011900 vs. TAIR 10
Match: AT2G38150.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 285.4 bits (729), Expect = 7.2e-77
Identity = 164/400 (41.00%), Postives = 231/400 (57.75%), Query Frame = 0

Query: 16  LSSLPTSFLSLLLLFLLAYNGLHVFSISPPSPEK-TIPHPAPFSPQKRPTADSTSTHLLF 75
           LS       S+LLL+LL              P K  +P P P+ P       S+S ++  
Sbjct: 6   LSIFANFTFSVLLLYLLYIESPKSTLYDNNLPFKPNVPLPRPYGPM------SSSCNINS 65

Query: 76  SIHENHPPPVLKSNTSVFHHPHLKSRPAKRVGKHKRR---LRSLRSELKESDFSARIEEF 135
            +   H    L         P  K+   +R+   +R+   L  L+S  K   F  R+ + 
Sbjct: 66  VVDSEHKEKELDPLL-----PPRKASKNQRIDWFRRKLPELEILKSTTKSKSFHTRVLDL 125

Query: 136 FAANSCKLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDSAKGKQILS 195
           +  N C  +FFM W+S  +SF  RE+ AI ++F    N   CL I+SNSLDS  G  IL 
Sbjct: 126 YNKN-CSAQFFMIWLSPANSFGPREMLAIDTLFTT--NPGACLAILSNSLDSPNGYTILK 185

Query: 196 PFSEMGFSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLLYKF 255
           P  + GF+L+A++ D   + KNT AE W  +L+ G +  G I L  NLS+L RL +LYK+
Sbjct: 186 PLFDQGFNLIAVTIDIPFLVKNTPAEAWLKRLKSGNMDPGSIPLFMNLSDLTRLAVLYKY 245

Query: 256 GGIYIDTDVIILQNFTNLRNAIGAQTMDLKTGNWSRLNNAVMIFDKNHPLLLQFIKEFAT 315
           GG+Y+DTD+I L + T LRNAIGAQ+ D  T  W+RLNNAVM+FD  HPL+ +F++E+AT
Sbjct: 246 GGVYLDTDIIFLNDMTGLRNAIGAQSSDPATKRWTRLNNAVMVFDIYHPLMREFLQEYAT 305

Query: 316 TFDGNKWGHNGPYLVSRVISRL-NQNSEFNLTILPPSAFYPVVWNRIKTFFQGPKDAVHL 375
           TFDGNKWG+N PYLVSRVI RL N+    NLTI  P AFYPV W +I+  F+ P      
Sbjct: 306 TFDGNKWGYNSPYLVSRVIKRLGNKPGYNNLTIFSPDAFYPVNWIKIQKLFKKPATTREA 365

Query: 376 KWIIAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVN 411
           KW+   ++ +   S  +HLWN  +RK+++E+GS++  +V+
Sbjct: 366 KWVEKTVQDMNKGSYMIHLWNKVTRKIKIEEGSVMHTLVS 391

BLAST of CsGy1G011900 vs. TAIR 10
Match: AT2G38152.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.4e-75
Identity = 137/277 (49.46%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 138 KLRFFMTWISSLDSFSDRELWAIQSIFKVHDNENPCLIIVSNSLDSAKGKQILSPFSEMG 197
           ++RFFMTW S  + F  RE+ A++S+FK H     CL+IVS SLDS +G  IL P ++ G
Sbjct: 98  EVRFFMTWFSPAEYFGKREMLAVESVFKAH--PQGCLMIVSGSLDSLQGDSILKPLNDRG 157

Query: 198 FSLLAISPDFDAIFKNTEAELWFNQLQQGIVKAGEISLGQNLSNLLRLTLLYKFGGIYID 257
           + + A +PD   + +NT A+ WF +++      G I L QNLSNL RL  LYK+GG+Y+D
Sbjct: 158 YKVFAATPDMSLLLENTPAKSWFQEMKSCKRDPGRIPLHQNLSNLARLAFLYKYGGVYLD 217

Query: 258 TDVIILQNFTNLRNAIGAQT-MDLKTGNWSRLNNAVMIFDKNHPLLLQFIKEFATTFDGN 317
           TD I+ ++F  L+N+IGAQT ++  + NW+RLNNAV+IF+K+HPL+  FI+EFA+TFDGN
Sbjct: 218 TDFIVTRSFKGLKNSIGAQTVVEGDSKNWTRLNNAVLIFEKDHPLVYSFIEEFASTFDGN 277

Query: 318 KWGHNGPYLVSRVISRLNQNSEFNLTILPPSAFYPVVWNRIKTFFQGPK---DAVHLKWI 377
           KWGHNGPYLV+RV  R  +    N T+LPP AFYP  W  I   FQ P+   D+  LK  
Sbjct: 278 KWGHNGPYLVTRVAQRARETIGDNFTVLPPVAFYPFNWLDIPRLFQTPRGSNDSTLLKTD 337

Query: 378 IAKLRHIQTKSLALHLWNNHSRKLQVEKGSIVDIIVN 411
           + KL     +S  LHLWN  +RKL++ KGS++DII++
Sbjct: 338 LVKLNR---ESYGLHLWNKITRKLKIGKGSVIDIIIS 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C8Q45.1e-2729.55Uncharacterized protein At4g19900 OS=Arabidopsis thaliana OX=3702 GN=At4g19900 P... [more]
Q9JI931.4e-2431.98Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=... [more]
Q67BJ41.2e-2330.18Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4gal... [more]
Q9N2914.5e-2331.08Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4G... [more]
Q9NPC47.6e-2330.63Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT... [more]
Match NameE-valueIdentityDescription
XP_008442286.17.09e-27791.99PREDICTED: lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo] >KAA005... [more]
XP_031738021.18.30e-25387.89lactosylceramide 4-alpha-galactosyltransferase [Cucumis sativus][more]
XP_038905948.13.39e-24080.00lactosylceramide 4-alpha-galactosyltransferase-like [Benincasa hispida][more]
KAG7019628.11.96e-22777.99hypothetical protein SDJN02_18591, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023001721.14.58e-22675.94lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5D3DSB73.43e-27791.99Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3B6373.43e-27791.99lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1KJE12.22e-22675.94lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1EH012.78e-21975.85lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=366... [more]
A0A6J1CG172.33e-17178.57uncharacterized protein At4g19900 OS=Momordica charantia OX=3673 GN=LOC111010894... [more]
Match NameE-valueIdentityDescription
AT1G61050.11.4e-9647.45alpha 1,4-glycosyltransferase family protein [more]
AT5G01250.18.8e-8351.07alpha 1,4-glycosyltransferase family protein [more]
AT3G09020.19.1e-8048.43alpha 1,4-glycosyltransferase family protein [more]
AT2G38150.17.2e-7741.00alpha 1,4-glycosyltransferase family protein [more]
AT2G38152.11.4e-7549.46alpha 1,4-glycosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007577Glycosyltransferase, DXD sugar-binding motifPFAMPF04488Gly_transf_sugcoord: 154..277
e-value: 4.1E-17
score: 62.6
IPR007652Alpha 1,4-glycosyltransferase domainPFAMPF04572Gb3_synthcoord: 295..409
e-value: 6.2E-26
score: 91.1
NoneNo IPR availableGENE3D3.90.550.20coord: 140..346
e-value: 3.4E-22
score: 81.2
NoneNo IPR availablePANTHERPTHR46781:SF5ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEINcoord: 8..410
IPR044789Putative alpha 1,4-glycosyltransferase, plantPANTHERPTHR46781ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEINcoord: 8..410
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 141..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G011900.2CsGy1G011900.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016757 glycosyltransferase activity