Cp4.1LG18g07420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g07420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionBifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
LocationCp4.1LG18: 7260070 .. 7262427 (+)
RNA-Seq ExpressionCp4.1LG18g07420
SyntenyCp4.1LG18g07420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTAATATCACGTTGATAATATGGCACTCTCTCACAAGTATTGAGGGAGGGAAGAACAAGGAGTGTGTGGAATGGCACACAGGCTGCATGGTTCAACTCACTTAAACAGTGTCCATCCCTCTATTTCTATAGAGTCAAAATCTGTATTGTCTCTGCACCATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTACTCTACCGATTGCCGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCGAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCGTGCAAGGTATGGCATCGATGCCACCGATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACATGTTCAGGTACAACTATAATCCTGTCATCCTAAAGCCTGTTAAGTATCTTCTTCTACTTCGTTCTAACATTTATAACTCTCTGAATGGTCATTTGAAGCTCTCCTTGAGCCACCAAGTCCTCCCAGTTCTCATGTTTCTCCTAGAGTAAACACCAACACCACAATTACCAATGCTACGGTTCTAGGTATAGTTTTTGGTTCAGCTCTACTACAAGCACGACATGTTATACATTGTGACACTACTTTTATGGATTTCAGCTACTCCAGTGGGTCAACCAACACCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCAAAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGAAACCAATTATCTAAAATCTCGTTCAACATTATATGCTCTTCTTTATTAGATTCCCCGTAGGTTGTTTTGGTTCAATAACGTTGTTTCTCTTAGCAGCTGACAGAAAAATGGTTCCCTGTTTTCAGATAAGTCTTGTTCCTTTGTCTCCCTAAAACAGTCAGCCTAAGGAGAGAAATCTAAGTAAAAGGCCATAGCAGATAGACAGAATGAAGTAAATGAGAATGGCCTAAAAGAAGAACAATATGCTGACAAGAAAACTAAGACATGCAAAATCAAAATCTTTACTAGATTCAATAGAATAAGGTTTTCATAACATACCTAGAAAGCTTATGGCATTACAAATTCACACAGTATTCTTTGTTTCCTTTTCACTTCTTTAACTGGTTACGAGGATGATAATGGATTCAAGATGTGGGGTCTGTTTTTGATATCGAAATATCAAGATCATTCCAAACTAGGAGCCACCTCTGTTGGATTAGATTCAAAGACATCTGGGTTTGATGCCTCGGGTTCTTCTAATATCTCTTGAGTTTGAACTGATTCTTTGTATGCAGGCGTGTCCTTGAAATCAGTGGCTCCTTCGTTGTATGTAGTGACAGCAATCCCACATGCAATTACCTATTGTAATGATGAATGAATTAGTGAATCGCCCATTTGCGAAAGGATAACAGAACATGAGCTATCTATTTTATCCTTTTCTAGAAAAACTACCCAAAAGAAAACATCTAATTGATGTTAGATCATGATTCTGTTCTTGTTATGTTGAAGAAAAAGCTGAGTTGCTGGTTAATTTAGTGTTTGGGCCTCGCTTATCTCAAATCTCATCTGTCAATTACAGAGGCAACTAGATTTCTGGAACTAAGTTATTAATCTGAATCTTTTGCTGAATGCTATCTAAGATTAGAACAGACAACAGAATCAATCAAATAATTATAGGACATAATTGGCGAAATTAAAGATAGGGAAAAATCGAACCGCGAAAGCGATGCCAAAGGCCCACAAGTACTCGAGGAAGAAGCCGAGGGGATCCCACTGCTGACCCTCCCAGAAATCAGGCTGGAGCCCAGGCAATTCCGGCAAATTTTCATCCACATCAAACGCTACACCGGCATTTTCAGCAATTTCGGCCATCCGATTTCGTTCATCTTCTTGTTGTTCTTCCTGTTCCTTGGTTGGCGTCGAGCTCCTCCGGTTCTTACTGTCGATTCGGCCAATTCGGAAGGAGGATTTCTTTGAATGAACGACATGGAGGGTGCGGGCTTCTTTAGACGGCGAAAAGAGCGCGTTGCGACGGAAAAGGAATTGGTTGGAAGGAGTTCTGGTGGAGGTTCTATAGAGAATTCCCCCGCCGGTGCCACAAGCGGCGCCGCCATTGGTTATGGTCAGAGCCATTTGAGTGGAGGAGAGTGAGCTCTGAACACCCAGAAAAATGGAGGAGGAGGATTGTGCGGATAA

mRNA sequence

ATCTAATATCACGTTGATAATATGGCACTCTCTCACAAGTATTGAGGGAGGGAAGAACAAGGAGTGTGTGGAATGGCACACAGGCTGCATGGTTCAACTCACTTAAACAGTGTCCATCCCTCTATTTCTATAGAGTCAAAATCTGTATTGTCTCTGCACCATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTACTCTACCGATTGCCGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCGAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCGTGCAAGGTATGGCATCGATGCCACCGATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACATGTTCAGCTCTCCTTGAGCCACCAAGTCCTCCCAGTTCTCATGTTTCTCCTAGAGTAAACACCAACACCACAATTACCAATGCTACGGTTCTAGCTACTCCAGTGGGTCAACCAACACCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCAAAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGAAACCAATTATCTAAAATCTCGTTCAACATTATATGCTCTTCTTTATTAGATTCCCCGTAGGTTGTTTTGGTTCAATAACGTTGTTTCTCTTAGCAGCTGACAGAAAAATGGTTCCCTGTTTTCAGATAAGTCTTGTTCCTTTGTCTCCCTAAAACAGTCAGCCTAAGGAGAGAAATCTAAGTAAAAGGCCATAGCAGATAGACAGAATGAAGTAAATGAGAATGGCCTAAAAGAAGAACAATATGCTGACAAGAAAACTAAGACATGCAAAATCAAAATCTTTACTAGATTCAATAGAATAAGGTTTTCATAACATACCTAGAAAGCTTATGGCATTACAAATTCACACAGTATTCTTTGTTTCCTTTTCACTTCTTTAACTGGTTACGAGGATGATAATGGATTCAAGATGTGGGGTCTGTTTTTGATATCGAAATATCAAGATCATTCCAAACTAGGAGCCACCTCTGTTGGATTAGATTCAAAGACATCTGGGTTTGATGCCTCGGGTTCTTCTAATATCTCTTGAGTTTGAACTGATTCTTTGTATGCAGGCGTGTCCTTGAAATCAGTGGCTCCTTCGTTGTATGTAGTGACAGCAATCCCACATGCAATTACCTATTGTAATGATGAATGAATTAGTGAATCGCCCATTTGCGAAAGGATAACAGAACATGAGCTATCTATTTTATCCTTTTCTAGAAAAACTACCCAAAAGAAAACATCTAATTGATGTTAGATCATGATTCTGTTCTTGTTATGTTGAAGAAAAAGCTGAGTTGCTGGTTAATTTAGTGTTTGGGCCTCGCTTATCTCAAATCTCATCTGTCAATTACAGAGGCAACTAGATTTCTGGAACTAAGTTATTAATCTGAATCTTTTGCTGAATGCTATCTAAGATTAGAACAGACAACAGAATCAATCAAATAATTATAGGACATAATTGGCGAAATTAAAGATAGGGAAAAATCGAACCGCGAAAGCGATGCCAAAGGCCCACAAGTACTCGAGGAAGAAGCCGAGGGGATCCCACTGCTGACCCTCCCAGAAATCAGGCTGGAGCCCAGGCAATTCCGGCAAATTTTCATCCACATCAAACGCTACACCGGCATTTTCAGCAATTTCGGCCATCCGATTTCGTTCATCTTCTTGTTGTTCTTCCTGTTCCTTGGTTGGCGTCGAGCTCCTCCGGTTCTTACTGTCGATTCGGCCAATTCGGAAGGAGGATTTCTTTGAATGAACGACATGGAGGGTGCGGGCTTCTTTAGACGGCGAAAAGAGCGCGTTGCGACGGAAAAGGAATTGGTTGGAAGGAGTTCTGGTGGAGGTTCTATAGAGAATTCCCCCGCCGGTGCCACAAGCGGCGCCGCCATTGGTTATGGTCAGAGCCATTTGAGTGGAGGAGAGTGAGCTCTGAACACCCAGAAAAATGGAGGAGGAGGATTGTGCGGATAA

Coding sequence (CDS)

ATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTACTCTACCGATTGCCGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCGAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCGTGCAAGGTATGGCATCGATGCCACCGATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACATGTTCAGCTCTCCTTGAGCCACCAAGTCCTCCCAGTTCTCATGTTTCTCCTAGAGTAAACACCAACACCACAATTACCAATGCTACGGTTCTAGCTACTCCAGTGGGTCAACCAACACCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCAAAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGA

Protein sequence

MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPPSPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVIAMKTFLFLLVLH
Homology
BLAST of Cp4.1LG18g07420 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-20
Identity = 70/167 (41.92%), Postives = 93/167 (55.69%), Query Frame = 0

Query: 36  AQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGT-TLS-SFPIN 95
           A C + LL LA C PFVQG A +P   CC +L Q+Y+   +CLCL LN T TLS +FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 96  TTRATQLPDICSLQVNISTCSALL--EPPS-------PPSSHVSPRVNTNTTITNATVLA 155
            T A QLP +C++  N STCS+    E PS       PPSS    +++      N+ V A
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAK-NNSRVAA 147

Query: 156 TPVGQPTPKP-SMVGLGLGSISASFKPRAGIQLKVVIAMKTFLFLLV 191
           TPV Q  P+P S +GLG G  S+  K    + +  + A+     LL+
Sbjct: 148 TPVAQMAPRPTSFMGLGYGLKSSGSKSEIQLTIFALAAILPAALLLI 193

BLAST of Cp4.1LG18g07420 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.5e-09
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 0

Query: 11  TLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQL 70
           T + L+ V + +A + D T       +CT  L+ +A+C P+VQG A  P   CC  LKQ+
Sbjct: 11  TAIALIVVAMVVAAADDKTKDK---EECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 70

Query: 71  YTSVPSCLCLLL---NGTTLSSFPINTTRATQLPDICSLQVNISTCSALLE-PPSPPSSH 128
             S   CLC+++   N   L    IN + A  LP +C    +++ C ALL   P+ P + 
Sbjct: 71  LNSNKKCLCVIIQDRNDPDL-GLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQ 127

BLAST of Cp4.1LG18g07420 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 45/135 (33.33%), Postives = 68/135 (50.37%), Query Frame = 0

Query: 37  QCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLL---NGTTLSSFPIN 96
           +CTA L+ +A+C P+VQG A  P   CC  LKQ+  S   CLC+++   N   L    +N
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDL-GLQVN 94

Query: 97  TTRATQLPDICSLQVNISTCSALLE-PPSPPSSHVSPRV--NTNTTITNATVLATPVGQP 156
            + A  LP +C    +I+ C ALL   P+ P + V  ++    N T+ +A+       +P
Sbjct: 95  VSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETV-SASAPTGSASEP 154

Query: 157 TPKPSMVGLGLGSIS 166
           T   S  G   G+ S
Sbjct: 155 TSMSSTPGSSAGNNS 167

BLAST of Cp4.1LG18g07420 vs. ExPASy Swiss-Prot
Match: Q9LJ85 (Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3702 GN=LTPG20 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.7e-06
Identity = 45/155 (29.03%), Postives = 67/155 (43.23%), Query Frame = 0

Query: 10  LTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLAS-CAPFVQGMAS---MPPMTCCG 69
           L +  +  + LPI   Q P      ++QCT S++   S C  F+   +S    P   CC 
Sbjct: 7   LVVAMIAVLALPIRGQQQP------LSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCCN 66

Query: 70  NLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPPSPP-- 129
           +L+ L T    CLCL++ GT   + PIN T A  LP  C++      C A + P + P  
Sbjct: 67  SLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPGP 126

Query: 130 ----SSHVSPRVNTNTTITNATVLATPVGQPTPKP 155
                  +SP   T+  +   T  A      T +P
Sbjct: 127 AATFGPSMSPGPETDPIVPEPTPAAQTPQSDTTRP 155

BLAST of Cp4.1LG18g07420 vs. ExPASy Swiss-Prot
Match: Q1PFD8 (Non-specific lipid transfer protein GPI-anchored 9 OS=Arabidopsis thaliana OX=3702 GN=LTPG9 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.6e-05
Identity = 31/112 (27.68%), Postives = 53/112 (47.32%), Query Frame = 0

Query: 32  SPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNG-TTLSSF 91
           +P +  C   LLP   C P++  +   PP +CCG +K++      CLC+  N    L + 
Sbjct: 27  NPQLTACLQKLLP---CQPYIHSLNPPPPPSCCGPMKEIVEKDAPCLCIAFNNPEVLKAL 86

Query: 92  PINTTRATQLPDICSLQVNISTCSALLEPPSPPSSHVSPRVNTNTTITNATV 143
            +    A  LP  C +  ++S CS +  P     S ++   +TN T + +T+
Sbjct: 87  NLTKENALLLPKACGVNPDVSLCSKIATP-----SPIASPGSTNGTSSASTI 130

BLAST of Cp4.1LG18g07420 vs. NCBI nr
Match: XP_023515373.1 (protein YLS3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365 bits (938), Expect = 3.71e-127
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI
Sbjct: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

BLAST of Cp4.1LG18g07420 vs. NCBI nr
Match: XP_022922067.1 (protein YLS3 [Cucurbita moschata])

HSP 1 Score: 357 bits (916), Expect = 8.39e-124
Identity = 187/192 (97.40%), Postives = 190/192 (98.96%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLLFLLHV LPIAVSQDPTLSSPTIAQCTASLLPLASCAPF+QGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRV+TNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL VVI
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

BLAST of Cp4.1LG18g07420 vs. NCBI nr
Match: KAG6589554.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357 bits (915), Expect = 1.19e-123
Identity = 187/192 (97.40%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLLFLLHV LPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRV+TNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL V I
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

BLAST of Cp4.1LG18g07420 vs. NCBI nr
Match: KAG7023240.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357 bits (915), Expect = 3.32e-123
Identity = 187/192 (97.40%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLLFLLHV LPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP
Sbjct: 30  MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 89

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNISTCSALLEPP
Sbjct: 90  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 149

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRV+TNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL V I
Sbjct: 150 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 209

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFLLVLH
Sbjct: 210 AMKTFLFLLVLH 221

BLAST of Cp4.1LG18g07420 vs. NCBI nr
Match: XP_022987249.1 (protein YLS3 [Cucurbita maxima])

HSP 1 Score: 344 bits (883), Expect = 9.00e-119
Identity = 181/192 (94.27%), Postives = 184/192 (95.83%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLL LLHVTLPIAVSQDPT SSPTIAQCT SLL LASCAPFVQGMAS PP
Sbjct: 1   MASLPYLPCLTLLLLLHVTLPIAVSQDPTPSSPTIAQCTTSLLQLASCAPFVQGMASTPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNIS CSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISACSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRVNTN+TITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL V I
Sbjct: 121 SPPSSHVSPRVNTNSTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFL+VLH
Sbjct: 181 AMKTFLFLVVLH 192

BLAST of Cp4.1LG18g07420 vs. ExPASy TrEMBL
Match: A0A6J1E230 (protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1)

HSP 1 Score: 357 bits (916), Expect = 4.06e-124
Identity = 187/192 (97.40%), Postives = 190/192 (98.96%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLLFLLHV LPIAVSQDPTLSSPTIAQCTASLLPLASCAPF+QGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRV+TNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL VVI
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

BLAST of Cp4.1LG18g07420 vs. ExPASy TrEMBL
Match: A0A6J1JIC4 (protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1)

HSP 1 Score: 344 bits (883), Expect = 4.36e-119
Identity = 181/192 (94.27%), Postives = 184/192 (95.83%), Query Frame = 0

Query: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60
           MASLPYLPCLTLL LLHVTLPIAVSQDPT SSPTIAQCT SLL LASCAPFVQGMAS PP
Sbjct: 1   MASLPYLPCLTLLLLLHVTLPIAVSQDPTPSSPTIAQCTTSLLQLASCAPFVQGMASTPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNIS CSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISACSALLEPP 120

Query: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180
           SPPSSHVSPRVNTN+TITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL V I
Sbjct: 121 SPPSSHVSPRVNTNSTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 192
           AMKTFLFL+VLH
Sbjct: 181 AMKTFLFLVVLH 192

BLAST of Cp4.1LG18g07420 vs. ExPASy TrEMBL
Match: A0A5A7TH96 (Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 PE=4 SV=1)

HSP 1 Score: 273 bits (697), Expect = 1.16e-90
Identity = 150/199 (75.38%), Postives = 166/199 (83.42%), Query Frame = 0

Query: 1   MASLPYLPC-------LTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQ 60
           MASLPYLP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPFVQ
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLN T LSSFPINTTRA QLPD+CSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL  PSPPSSHV PRV+ N+TI+NATVLA+PV QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGG 180

Query: 181 IQLKVVIAMKTFLFLLVLH 192
           IQL  VIA+ TFLFL+VL+
Sbjct: 181 IQLTAVIAITTFLFLVVLY 198

BLAST of Cp4.1LG18g07420 vs. ExPASy TrEMBL
Match: A0A1S3BVJ6 (protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1)

HSP 1 Score: 273 bits (697), Expect = 1.16e-90
Identity = 150/199 (75.38%), Postives = 166/199 (83.42%), Query Frame = 0

Query: 1   MASLPYLPC-------LTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQ 60
           MASLPYLP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPFVQ
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLN T LSSFPINTTRA QLPD+CSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL  PSPPSSHV PRV+ N+TI+NATVLA+PV QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGG 180

Query: 181 IQLKVVIAMKTFLFLLVLH 192
           IQL  VIA+ TFLFL+VL+
Sbjct: 181 IQLTAVIAITTFLFLVVLY 198

BLAST of Cp4.1LG18g07420 vs. ExPASy TrEMBL
Match: A0A0A0LSN1 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV=1)

HSP 1 Score: 261 bits (667), Expect = 4.56e-86
Identity = 144/196 (73.47%), Postives = 161/196 (82.14%), Query Frame = 0

Query: 1   MASLPYLPC-------LTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQ 60
           MASLP+LP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPFVQ
Sbjct: 1   MASLPHLPLPQQAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLNGT LSSFPINTTRA QLPDICSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL PPSPPSSHV PRV+ N+TI+NATVLA+P+ QPTPKPS+VGLGLG ISASFK + G
Sbjct: 121 SALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLKGG 180

Query: 181 IQLKVVIAMKTFLFLL 189
           IQL  +  M  F+ LL
Sbjct: 181 IQLTQLAVMFFFIRLL 195

BLAST of Cp4.1LG18g07420 vs. TAIR 10
Match: AT1G73890.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 9.0e-22
Identity = 70/167 (41.92%), Postives = 93/167 (55.69%), Query Frame = 0

Query: 36  AQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGT-TLS-SFPIN 95
           A C + LL LA C PFVQG A +P   CC +L Q+Y+   +CLCL LN T TLS +FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 96  TTRATQLPDICSLQVNISTCSALL--EPPS-------PPSSHVSPRVNTNTTITNATVLA 155
            T A QLP +C++  N STCS+    E PS       PPSS    +++      N+ V A
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAK-NNSRVAA 147

Query: 156 TPVGQPTPKP-SMVGLGLGSISASFKPRAGIQLKVVIAMKTFLFLLV 191
           TPV Q  P+P S +GLG G  S+  K    + +  + A+     LL+
Sbjct: 148 TPVAQMAPRPTSFMGLGYGLKSSGSKSEIQLTIFALAAILPAALLLI 193

BLAST of Cp4.1LG18g07420 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.6e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 0

Query: 11  TLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQL 70
           T + L+ V + +A + D T       +CT  L+ +A+C P+VQG A  P   CC  LKQ+
Sbjct: 11  TAIALIVVAMVVAAADDKTKDK---EECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 70

Query: 71  YTSVPSCLCLLL---NGTTLSSFPINTTRATQLPDICSLQVNISTCSALLE-PPSPPSSH 128
             S   CLC+++   N   L    IN + A  LP +C    +++ C ALL   P+ P + 
Sbjct: 71  LNSNKKCLCVIIQDRNDPDL-GLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQ 127

BLAST of Cp4.1LG18g07420 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 7.9e-10
Identity = 45/135 (33.33%), Postives = 68/135 (50.37%), Query Frame = 0

Query: 37  QCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLL---NGTTLSSFPIN 96
           +CTA L+ +A+C P+VQG A  P   CC  LKQ+  S   CLC+++   N   L    +N
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDL-GLQVN 94

Query: 97  TTRATQLPDICSLQVNISTCSALLE-PPSPPSSHVSPRV--NTNTTITNATVLATPVGQP 156
            + A  LP +C    +I+ C ALL   P+ P + V  ++    N T+ +A+       +P
Sbjct: 95  VSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETV-SASAPTGSASEP 154

Query: 157 TPKPSMVGLGLGSIS 166
           T   S  G   G+ S
Sbjct: 155 TSMSSTPGSSAGNNS 167

BLAST of Cp4.1LG18g07420 vs. TAIR 10
Match: AT3G22620.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 4.8e-07
Identity = 45/155 (29.03%), Postives = 67/155 (43.23%), Query Frame = 0

Query: 10  LTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLAS-CAPFVQGMAS---MPPMTCCG 69
           L +  +  + LPI   Q P      ++QCT S++   S C  F+   +S    P   CC 
Sbjct: 7   LVVAMIAVLALPIRGQQQP------LSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCCN 66

Query: 70  NLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPPSPP-- 129
           +L+ L T    CLCL++ GT   + PIN T A  LP  C++      C A + P + P  
Sbjct: 67  SLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPGP 126

Query: 130 ----SSHVSPRVNTNTTITNATVLATPVGQPTPKP 155
                  +SP   T+  +   T  A      T +P
Sbjct: 127 AATFGPSMSPGPETDPIVPEPTPAAQTPQSDTTRP 155

BLAST of Cp4.1LG18g07420 vs. TAIR 10
Match: AT1G73560.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.8e-06
Identity = 31/112 (27.68%), Postives = 53/112 (47.32%), Query Frame = 0

Query: 32  SPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNG-TTLSSF 91
           +P +  C   LLP   C P++  +   PP +CCG +K++      CLC+  N    L + 
Sbjct: 27  NPQLTACLQKLLP---CQPYIHSLNPPPPPSCCGPMKEIVEKDAPCLCIAFNNPEVLKAL 86

Query: 92  PINTTRATQLPDICSLQVNISTCSALLEPPSPPSSHVSPRVNTNTTITNATV 143
            +    A  LP  C +  ++S CS +  P     S ++   +TN T + +T+
Sbjct: 87  NLTKENALLLPKACGVNPDVSLCSKIATP-----SPIASPGSTNGTSSASTI 130

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9B11.3e-2041.92Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3... [more]
O648656.5e-0933.88Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3... [more]
O648641.1e-0833.33Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3... [more]
Q9LJ856.7e-0629.03Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3... [more]
Q1PFD82.6e-0527.68Non-specific lipid transfer protein GPI-anchored 9 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
XP_023515373.13.71e-127100.00protein YLS3 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022922067.18.39e-12497.40protein YLS3 [Cucurbita moschata][more]
KAG6589554.11.19e-12397.40Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7023240.13.32e-12397.40Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022987249.19.00e-11994.27protein YLS3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E2304.06e-12497.40protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1[more]
A0A6J1JIC44.36e-11994.27protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1[more]
A0A5A7TH961.16e-9075.38Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 P... [more]
A0A1S3BVJ61.16e-9075.38protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1[more]
A0A0A0LSN14.56e-8673.47AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT1G73890.19.0e-2241.92Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G44300.14.6e-1033.88Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G44290.17.9e-1033.33Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G22620.14.8e-0729.03Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G73560.11.8e-0627.68Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 38..113
e-value: 5.6E-4
score: 29.2
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 24..113
e-value: 1.3E-14
score: 54.1
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 34..113
e-value: 1.9E-14
score: 55.2
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 37..113
IPR043325Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) ProteinPANTHERPTHR33044BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATEDcoord: 14..140
NoneNo IPR availablePANTHERPTHR33044:SF11BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEINcoord: 14..140
NoneNo IPR availableCDDcd00010AAI_LTSScoord: 45..106
e-value: 1.15529E-13
score: 60.9035

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g07420.1Cp4.1LG18g07420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane