Cp4.1LG17g09930 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g09930
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cysteine proteinase inhibitor B
Location	Cp4.1LG17: 7599752 .. 7600150 (+)
RNA-Seq Expression	Cp4.1LG17g09930
Synteny	Cp4.1LG17g09930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAAAGCAAGGCTCTCTCTGATCGCCGTCGTGGTACTGGCGCTGCTGGAGGTGGCGGTGCTGGCGGAAGGATACAGCGGAAGAATAGGCGGACGAAGAAGAATCAAAGACGTGAGGAGCAACGAGGAGGTGCAGGGATTAGGGAGGTTCAGCGTGGAGGAGTACAATCGGAGATCGCGGCGGAGTGGAAGCGGAGAGGTGAAGTTCTTGGCGGTGTTGGCGGCGGAGAGGCAGGTGGTGGCCGGAACGAAGTATTACTTGAGGATCTTGGGGAGTGAGAATGGTCGGAAGAGGATTTTTGAATCGGTTGTGGTCGTGAAGCCGTGGCTAAGGTCTAAGCAGCTTATCGATTTCTCGGCGTCGAGATTGTTTCCGAGTAGGATTTCAAATTTCTGA

mRNA sequence

ATGACGAAAGCAAGGCTCTCTCTGATCGCCGTCGTGGTACTGGCGCTGCTGGAGGTGGCGGTGCTGGCGGAAGGATACAGCGGAAGAATAGGCGGACGAAGAAGAATCAAAGACGTGAGGAGCAACGAGGAGGTGCAGGGATTAGGGAGGTTCAGCGTGGAGGAGTACAATCGGAGATCGCGGCGGAGTGGAAGCGGAGAGGTGAAGTTCTTGGCGGTGTTGGCGGCGGAGAGGCAGGTGGTGGCCGGAACGAAGTATTACTTGAGGATCTTGGGGAGTGAGAATGGTCGGAAGAGGATTTTTGAATCGGTTGTGGTCGTGAAGCCGTGGCTAAGGTCTAAGCAGCTTATCGATTTCTCGGCGTCGAGATTGTTTCCGAGTAGGATTTCAAATTTCTGA

Coding sequence (CDS)

ATGACGAAAGCAAGGCTCTCTCTGATCGCCGTCGTGGTACTGGCGCTGCTGGAGGTGGCGGTGCTGGCGGAAGGATACAGCGGAAGAATAGGCGGACGAAGAAGAATCAAAGACGTGAGGAGCAACGAGGAGGTGCAGGGATTAGGGAGGTTCAGCGTGGAGGAGTACAATCGGAGATCGCGGCGGAGTGGAAGCGGAGAGGTGAAGTTCTTGGCGGTGTTGGCGGCGGAGAGGCAGGTGGTGGCCGGAACGAAGTATTACTTGAGGATCTTGGGGAGTGAGAATGGTCGGAAGAGGATTTTTGAATCGGTTGTGGTCGTGAAGCCGTGGCTAAGGTCTAAGCAGCTTATCGATTTCTCGGCGTCGAGATTGTTTCCGAGTAGGATTTCAAATTTCTGA

Protein sequence

MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFSASRLFPSRISNF

Homology

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BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot
Match: Q5N806 (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-20
Identity = 54/112 (48.21%), Postives = 73/112 (65.18%), Query Frame = 0


Query: 25  GYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSGEVK--FLAVLAAERQVVA 84
           G   R+GGR  ++DV  + EVQ LGRFSVEE+NRR R    G+V+  F  V+AA+RQVV+
Sbjct: 45  GAGRRVGGRTEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGDVRLEFGRVVAAQRQVVS 104

Query: 85  GTKYYLRI-------LGSENGRKRIFESVVVVKPWLRSKQLIDFSASRLFPS 128
           G KYYLR+        G   G  R+F++VVVVKPWL S+ L+ F+ +   P+
Sbjct: 105 GLKYYLRVAAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAPAADSPN 156

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BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot
Match: Q10993 (Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-18
Identity = 45/92 (48.91%), Postives = 73/92 (79.35%), Query Frame = 0


Query: 31  GGRRRIKDVRSNEEVQGLGRFSVEEYNR--RSRRSGSGEVKFLAVLAAERQVVAGTKYYL 90
           GGR ++K+V+++ E+Q LG +SV+EYNR  R++++G+G++KF  V+AAE QVVAGTKYYL
Sbjct: 3   GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYL 62

Query: 91  RILG-SENGRKRIFESVVVVKPWLRSKQLIDF 120
           +I   ++ G+ ++F++ VVV+ W  SK+L+ F
Sbjct: 63  KIEAITKGGKMKVFDAEVVVQSWKHSKKLLGF 94

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BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot
Match: A2XS65 (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_014908 PE=3 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 3.4e-17
Identity = 48/107 (44.86%), Postives = 69/107 (64.49%), Query Frame = 0


Query: 30  IGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSG--------EVKFLAVLAAERQVV 89
           +GGR  I+DV SN+ VQ LGRF+V E+NRR R  GSG        ++ F  V+ A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 90  AGTKYYLRIL--------GSENGRKRIFESVVVVKPWLRSKQLIDFS 121
           +G  YYL++         G+  G  R+F++VVVVK WL+SK+L+ F+
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFT 145

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BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot
Match: P0C579 (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0350100 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.4e-17
Identity = 48/107 (44.86%), Postives = 69/107 (64.49%), Query Frame = 0


Query: 30  IGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSG--------EVKFLAVLAAERQVV 89
           +GGR  I+DV SN+ VQ LGRF+V E+NRR R  GSG        ++ F  V+ A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 90  AGTKYYLRIL--------GSENGRKRIFESVVVVKPWLRSKQLIDFS 121
           +G  YYL++         G+  G  R+F++VVVVK WL+SK+L+ F+
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFT 145

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BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot
Match: Q8L5T9 (Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=CYS2 PE=2 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 9.3e-15
Identity = 50/130 (38.46%), Postives = 82/130 (63.08%), Query Frame = 0


Query: 6   LSLIAVVVLALL-EVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSG 65
           LSL+  +V+A++   A      S  +GG+  + ++R+N E+Q LGR+ VE++N++++   
Sbjct: 11  LSLLGFLVIAVVTPSAANPFRKSVVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQ 70

Query: 66  -------------SGEVKFLAVLAAERQVVAGTKYYLRI-LGSENGRKRIFESVVVVKPW 121
                        S  ++F  V++A++QVVAG KYYLRI +   NG  R+F+SVVV++PW
Sbjct: 71  GNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNGSTRMFDSVVVIQPW 130

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BLAST of Cp4.1LG17g09930 vs. NCBI nr
Match: XP_023513886.1 (cysteine proteinase inhibitor B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246 bits (627), Expect = 1.07e-81
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS
Sbjct: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

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BLAST of Cp4.1LG17g09930 vs. NCBI nr
Match: KAG7025292.1 (hypothetical protein SDJN02_11787, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240 bits (612), Expect = 2.08e-79
Identity = 128/132 (96.97%), Postives = 129/132 (97.73%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVVLALLEVA+LAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVLALLEVAILAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           RRSG GEVKFLAVL AERQVVAGTKYYLRILG ENGRKRIFESVVVVKPWLRSKQLIDFS
Sbjct: 61  RRSGGGEVKFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLRSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

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BLAST of Cp4.1LG17g09930 vs. NCBI nr
Match: KAG6592889.1 (hypothetical protein SDJN03_12365, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 239 bits (609), Expect = 5.96e-79
Identity = 128/132 (96.97%), Postives = 129/132 (97.73%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           RRSG GEV+FLAVL AERQVVAGTKYYLRILG ENGRKRIFESVVVVKPWLRSKQLIDFS
Sbjct: 61  RRSGGGEVEFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLRSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

[Close]

BLAST of Cp4.1LG17g09930 vs. NCBI nr
Match: XP_022960190.1 (cysteine proteinase inhibitor B [Cucurbita moschata])

HSP 1 Score: 236 bits (602), Expect = 6.96e-78
Identity = 127/132 (96.21%), Postives = 128/132 (96.97%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           RRSG GEV+FLAVL AERQVVAGTKYYLRILG ENGRKRIFESVVVVKPWL SKQLIDFS
Sbjct: 61  RRSGGGEVEFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLGSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

[Close]

BLAST of Cp4.1LG17g09930 vs. NCBI nr
Match: XP_023005096.1 (cysteine proteinase inhibitor B-like [Cucurbita maxima])

HSP 1 Score: 228 bits (582), Expect = 7.81e-75
Identity = 121/132 (91.67%), Postives = 129/132 (97.73%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVV+ALLEVA+LAEGYSGRIGGRRRIKDVRSN+EVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVVALLEVALLAEGYSGRIGGRRRIKDVRSNKEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           R+SG G+V+FLAV+AAERQVVAGTKYYLRI+G ENGRKRIFESVVVVKPWL SKQLIDFS
Sbjct: 61  RQSGGGKVEFLAVVAAERQVVAGTKYYLRIVGIENGRKRIFESVVVVKPWLGSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

[Close]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL
Match: A0A6J1H8E3 (cysteine proteinase inhibitor B OS=Cucurbita moschata OX=3662 GN=LOC111461002 PE=4 SV=1)

HSP 1 Score: 236 bits (602), Expect = 3.37e-78
Identity = 127/132 (96.21%), Postives = 128/132 (96.97%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           RRSG GEV+FLAVL AERQVVAGTKYYLRILG ENGRKRIFESVVVVKPWL SKQLIDFS
Sbjct: 61  RRSGGGEVEFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLGSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

[Close]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL
Match: A0A6J1KTZ7 (cysteine proteinase inhibitor B-like OS=Cucurbita maxima OX=3661 GN=LOC111498186 PE=4 SV=1)

HSP 1 Score: 228 bits (582), Expect = 3.78e-75
Identity = 121/132 (91.67%), Postives = 129/132 (97.73%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           MTKARLSLIAVVV+ALLEVA+LAEGYSGRIGGRRRIKDVRSN+EVQGLGRFSVEEYNRRS
Sbjct: 1   MTKARLSLIAVVVVALLEVALLAEGYSGRIGGRRRIKDVRSNKEVQGLGRFSVEEYNRRS 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           R+SG G+V+FLAV+AAERQVVAGTKYYLRI+G ENGRKRIFESVVVVKPWL SKQLIDFS
Sbjct: 61  RQSGGGKVEFLAVVAAERQVVAGTKYYLRIVGIENGRKRIFESVVVVKPWLGSKQLIDFS 120

Query: 121 ASRLFPSRISNF 132
           ASRLFPSRISNF
Sbjct: 121 ASRLFPSRISNF 132

[Close]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL
Match: A0A0A0KC20 (Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1)

HSP 1 Score: 161 bits (407), Expect = 1.53e-48
Identity = 87/132 (65.91%), Postives = 107/132 (81.06%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           M K +LS++ VVVLA+  VA L EGY GR+GGR  +KDVR NEEVQ LGRFSVEEYNR  
Sbjct: 1   MAKTKLSVM-VVVLAVALVA-LVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNR-- 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           R  G GEVKF AV+AAERQVV+GTKYYLRILG++NG +++F+SVV+VKPW+ SK+L+DFS
Sbjct: 61  RMGGGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRLFPSRISNF 132
            S +F + I NF
Sbjct: 121 PSAVFRTPIFNF 128

[Close]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL
Match: A0A1S3CPD1 (cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=1)

HSP 1 Score: 153 bits (387), Expect = 1.69e-45
Identity = 80/132 (60.61%), Postives = 103/132 (78.03%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           M K +LS++  +V A + + V+ EGY G++GGR  IKDV+ NEEVQ LGRFSVEEYNR  
Sbjct: 1   MAKTKLSVM--LVAAAVALVVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNR-- 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFS 120
           R  G GEVKF AV+AA+RQVV+GTKYYLRILG +NG ++IF+SVV+VKPW+ SK+L+DFS
Sbjct: 61  RMGGGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRLFPSRISNF 132
            S +F +  S F
Sbjct: 121 PSTVFSTPFSYF 128

[Close]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL
Match: A0A6J1DAN3 (cysteine proteinase inhibitor B OS=Momordica charantia OX=3673 GN=LOC111018574 PE=4 SV=1)

HSP 1 Score: 138 bits (348), Expect = 1.44e-39
Identity = 75/125 (60.00%), Postives = 95/125 (76.00%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           M + RL + A + +A + +A  AEGY GR+GGR  I+DVR+NEEVQ LGRF VEEYNR  
Sbjct: 1   MARRRL-IAAAIAIAAVTLAAAAEGYGGRLGGRTEIEDVRTNEEVQELGRFCVEEYNR-- 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRKR-IFESVVVVKPWLRSKQLIDF 120
           RR+G GEV+F AV+AAE+QVVAG KYYL+I GS  G    +F+S+V+VKPWLRSKQL+ F
Sbjct: 61  RRNGGGEVRFRAVVAAEKQVVAGMKYYLKISGSVKGESEMLFDSIVIVKPWLRSKQLLHF 120

Query: 121 SASRL 124
           S S +
Sbjct: 121 SPSTI 122

[Close]

BLAST of Cp4.1LG17g09930 vs. TAIR 10
Match: AT2G31980.1 (PHYTOCYSTATIN 2 )

HSP 1 Score: 81.3 bits (199), Expect = 6.6e-16
Identity = 50/130 (38.46%), Postives = 82/130 (63.08%), Query Frame = 0


Query: 6   LSLIAVVVLALL-EVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSG 65
           LSL+  +V+A++   A      S  +GG+  + ++R+N E+Q LGR+ VE++N++++   
Sbjct: 11  LSLLGFLVIAVVTPSAANPFRKSVVLGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQ 70

Query: 66  -------------SGEVKFLAVLAAERQVVAGTKYYLRI-LGSENGRKRIFESVVVVKPW 121
                        S  ++F  V++A++QVVAG KYYLRI +   NG  R+F+SVVV++PW
Sbjct: 71  GNIGSIAKTDTAISNPLQFSRVVSAQKQVVAGLKYYLRIEVTQPNGSTRMFDSVVVIQPW 130

[Close]

BLAST of Cp4.1LG17g09930 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 62.8 bits (151), Expect = 2.4e-10
Identity = 35/102 (34.31%), Postives = 64/102 (62.75%), Query Frame = 0


Query: 30  IGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSGEVKFLAVLAAERQVVAGTKYYLR 89
           +GG   +   +++ EV+ L RF+V+E+N++     +  ++F  V+ A+ QVVAGT ++L 
Sbjct: 37  VGGVGDVPANQNSGEVESLARFAVDEHNKKE----NALLEFARVVKAKEQVVAGTLHHLT 96

Query: 90  ILGSENGRKRIFESVVVVKPWLRSKQLIDFSASRLFPSRISN 132
           +   E G+K+++E+ V VKPWL  K+L +F  +   P+  S+
Sbjct: 97  LEILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

[Close]

BLAST of Cp4.1LG17g09930 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 62.8 bits (151), Expect = 2.4e-10
Identity = 35/102 (34.31%), Postives = 64/102 (62.75%), Query Frame = 0


Query: 30  IGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGSGEVKFLAVLAAERQVVAGTKYYLR 89
           +GG   +   +++ EV+ L RF+V+E+N++     +  ++F  V+ A+ QVVAGT ++L 
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKE----NALLEFARVVKAKEQVVAGTLHHLT 63

Query: 90  ILGSENGRKRIFESVVVVKPWLRSKQLIDFSASRLFPSRISN 132
           +   E G+K+++E+ V VKPWL  K+L +F  +   P+  S+
Sbjct: 64  LEILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 101

[Close]

BLAST of Cp4.1LG17g09930 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 2.4e-10
Identity = 44/122 (36.07%), Postives = 73/122 (59.84%), Query Frame = 0


Query: 1   MTKARLSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRS 60
           M    L  +++++L L+ V V   G  G +G R+ IK+V S+ +V  + ++++EE+N+ S
Sbjct: 1   MMMKSLICLSLILLPLVSV-VEGLGGGGGLGSRKPIKNV-SDPDVVAVAKYAIEEHNKES 60

Query: 61  RRSGSGEVKFLAVLAAERQVVAGTKYYLRILGSENGRK-RIFESVVVVKPWLRSKQLIDF 120
           +     ++ F+ V+    QVV+GTKY L+I   + G K + +E+VVV K WL SK L  F
Sbjct: 61  KE----KLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESF 116

Query: 121 SA 122
            A
Sbjct: 121 KA 116

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BLAST of Cp4.1LG17g09930 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 59.7 bits (143), Expect = 2.1e-09
Identity = 35/117 (29.91%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 6   LSLIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGS 65
           L L   + LA+      +   +  +GG   ++  +++ E++ L RF+++E+N++  +   
Sbjct: 11  LLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKI-- 70

Query: 66  GEVKFLAVLAAERQVVAGTKYYLRILGSENGRKRIFESVVVVKPWLRSKQLIDFSAS 123
             ++F  ++ A  QVVAGT Y+L +   E  + + FE+ V VKPW+  KQL +F  S
Sbjct: 71  --LEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g09930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5N806	1.5e-20	48.21	Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]
Q10993	2.4e-18	48.91	Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
A2XS65	3.4e-17	44.86	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_0...	[more]
P0C579	3.4e-17	44.86	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q8L5T9	9.3e-15	38.46	Cysteine proteinase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=CYS2 PE=2 SV=...	[more]

BLAST of Cp4.1LG17g09930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513886.1	1.07e-81	100.00	cysteine proteinase inhibitor B [Cucurbita pepo subsp. pepo]	[more]
KAG7025292.1	2.08e-79	96.97	hypothetical protein SDJN02_11787, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6592889.1	5.96e-79	96.97	hypothetical protein SDJN03_12365, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022960190.1	6.96e-78	96.21	cysteine proteinase inhibitor B [Cucurbita moschata]	[more]
XP_023005096.1	7.81e-75	91.67	cysteine proteinase inhibitor B-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG17g09930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8E3	3.37e-78	96.21	cysteine proteinase inhibitor B OS=Cucurbita moschata OX=3662 GN=LOC111461002 PE...	[more]
A0A6J1KTZ7	3.78e-75	91.67	cysteine proteinase inhibitor B-like OS=Cucurbita maxima OX=3661 GN=LOC111498186...	[more]
A0A0A0KC20	1.53e-48	65.91	Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1	[more]
A0A1S3CPD1	1.69e-45	60.61	cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=...	[more]
A0A6J1DAN3	1.44e-39	60.00	cysteine proteinase inhibitor B OS=Momordica charantia OX=3673 GN=LOC111018574 P...	[more]

BLAST of Cp4.1LG17g09930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31980.1	6.6e-16	38.46	PHYTOCYSTATIN 2	[more]
AT3G12490.2	2.4e-10	34.31	cystatin B	[more]
AT3G12490.1	2.4e-10	34.31	cystatin B	[more]
AT4G16500.1	2.4e-10	36.07	Cystatin/monellin superfamily protein	[more]
AT2G40880.1	2.1e-09	29.91	cystatin A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 28..122 e-value: 1.8E-6 score: 37.5
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 36..120 e-value: 1.4E-13 score: 51.0
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 42..119 e-value: 1.66533E-12 score: 57.484
None	No IPR available	GENE3D	3.10.450.10		coord: 28..123 e-value: 3.1E-26 score: 93.5
None	No IPR available	PANTHER	PTHR47373:SF1	CYSTEINE PROTEINASE INHIBITOR 2	coord: 7..122
None	No IPR available	PANTHER	PTHR47373	CYSTEINE PROTEINASE INHIBITOR 2	coord: 7..122
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 36..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g09930.1	Cp4.1LG17g09930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity