Cp4.1LG16g05470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g05470
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description(R)-specific enoyl-CoA hydratase
LocationCp4.1LG16: 6348612 .. 6354680 (+)
RNA-Seq ExpressionCp4.1LG16g05470
SyntenyCp4.1LG16g05470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAAAAAAAAAAAAAAAAAAAAACCTCGAAAAGGATAAGATTAATTCAACAGAGTACAGTAAGTGCTCATTTGAAGGAAGTCTGCGACGAAACGACACATCTTTGGTTATCTTACTGAGAGTATTACAACTCAGCTTCCCTCCATCTTTCAGTGGACCCATTTATTCAATACAACTAAAGGGCTACACCATTTCTCATAATCGTTGTAATTACAGGTAATCTGCTTTCTTTTACTTTCCAAATTTGTTCATTTCTCCCTTTGTTCTTTGAATCCTCGCTTTGCCCACAAGGTGTTTGATGAAATGCCAAGCCCAAGTNACCCTGGTGAGTCCGTGGTCTTCGGCACTCGGGTGAGTCCGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGGTGAGTCTGTGGTCTTCGGCACTCGGNTGAAATGCCAAGCCCAAGTTTTAATGACTTCGTTGAGCATCCTGGTTGTATAACCAAACACTTCTGAACATTCTTTTCTCTAGTGCTTTTAATGGCTGCCCTTTGTTCTCTTGTTACAGGCTTGTTTGTGGTAGACATTTGACTGATACTTATAGACCCTTTACAAGTATGCTAAGTAGGGGAGGTTTAGTCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAGTACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCGGCTTTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCATGTCATTTCCTCTCATTTTGTAAGTCTAGATGGTCTGCATTTCTTACACACAGAACTTTATGGGGACTGAAAAATGGCACTTACGCTTGTTTGAGAGCTTTTAATGTATGTGGATGATTGTAGCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATGGTTCGGGTTGAAGCAATCGACTTGAGAGAAATTAAGAAGAGATACCTGTAATAAGCTTGTTCTTGGCAAATCTCTACCTTCTCATTTGAATTTCAAGTTATCATTTCATTGAGTTGGGTACTTGCTGAATGTGTTTCAGGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGAGCCATCTTACCTTCTGGTTTTTGTTCCACAGATGGTTAGACGATGATGGTCTACGTCTCGACCGCTTCCTTGTACTTGGATCGAAGGGCTAGATGTGGTTGAAGGAGCAGAGTTTTCCATTTGAATTCATTCAATTTCTTCCTTGCTCTCTGTGGATAAAGGATGTCAAGCCTGGGAATAGGTCTGACCTCTATTTGCTTTTACTTCATCTCTTATTGATAGGTTATGAACAATATCTGGACCTGTTTCTTTCAATAAATTTGCCATGAGCCTTTTGATCTTGAAACTGATCCATCAATAGAGACTAACAAAATGAACAAATTTTCTTTCGTAATGAATTGAATTTTTATTGAACTCTCGTGTTCAATGAGACATCCAAGCAGTGTAAAAGTGTTAACGAATTTAAGAATCAGAAGATTCTGATTCGGTAATATTTCTATTGCAAGGAACAACTTGCCCCTTTGACAGCATCTTCACTAGACCCCTAACTACACTTAAACCTCATAATTTTCCAAAAAATGAAAAGGAAATAGAGAACTTTCTTTCTCCTGCGTACCTGGTAGAGGAGCAACGGAGCCGTTGTGTGGTATATACATCATCTGTAGTGAATTACAACATAGCTATCTCTTAGTCTGTGGCAGAATCACCGACGGTTTGAACGTCGAGATCTTCTGTGTCGCAATCGGAGGAAGTCTGATCTACAGAACAGTTGTTCAGTTGGCCTTGTGGGGTTCCAAAGAGGGTAGTGAATATCTGTCTGTGGCATTCGTCACAGAAGTAGATGTAGGTAAAATGGCCTTCATATGGGAGCTTATGGACTGCAGCTCCGGGTAGGATCCGATGAACGTAATCGGTCATTGATGGTGGAACCACCATATCATCCATCCCCTGTTTGAATGAAAATATGAGATCAAAAGGGAGAGGCAAACCAAACTACAATTCACAGCTCCAATTAGGATTTGAAAAACAAT

mRNA sequence

AACCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAAAAAAAAAAAAAAAAAAAAAACCTCGAAAAGGATAAGATTAATTCAACAGAGTACAGTAAGTGCTCATTTGAAGGAAGTCTGCGACGAAACGACACATCTTTGGTTATCTTACTGAGAGTATTACAACTCAGCTTCCCTCCATCTTTCAGTGGACCCATTTATTCAATACAACTAAAGGGCTACACCATTTCTCATAATCGTTGTAATTACAGGCTTGTTTGTGGTAGACATTTGACTGATACTTATAGACCCTTTACAAGTATGCTAAGTAGGGGAGGTTTAGTCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAGTACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCGGCTTTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCATGTCATTTCCTCTCATTTTCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATGGTTCGGGTTGAAGCAATCGACTTGAGAGAAATTAAGAAGAGATACCTGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGAGCCATCTTACCTTCTGGTTTTTGTTCCACAGATGGTTAGACGATGATGGTCTACGTCTCGACCGCTTCCTTGTACTTGGATCGAAGGGCTAGATGTGGTTGAAGGAGCAGAGTTTTCCATTTGAATTCATTCAATTTCTTCCTTGCTCTCTGTGGATAAAGGATGTCAAGCCTGGGAATAGGTCTGACCTCTATTTGCTTTTACTTCATCTCTTATTGATAGGTTATGAACAATATCTGGACCTGTTTCTTTCAATAAATTTGCCATGAGCCTTTTGATCTTGAAACTGATCCATCAATAGAGACTAACAAAATGAACAAATTTTCTTTCGTAATGAATTGAATTTTTATTGAACTCTCGTGTTCAATGAGACATCCAAGCAGTGTAAAAGTGTTAACGAATTTAAGAATCAGAAGATTCTGATTCGGTAATATTTCTATTGCAAGGAACAACTTGCCCCTTTGACAGCATCTTCACTAGACCCCTAACTACACTTAAACCTCATAATTTTCCAAAAAATGAAAAGGAAATAGAGAACTTTCTTTCTCCTGCGTACCTGGTAGAGGAGCAACGGAGCCGTTGTGTGGTATATACATCATCTGTAGTGAATTACAACATAGCTATCTCTTAGTCTGTGGCAGAATCACCGACGGTTTGAACGTCGAGATCTTCTGTGTCGCAATCGGAGGAAGTCTGATCTACAGAACAGTTGTTCAGTTGGCCTTGTGGGGTTCCAAAGAGGGTAGTGAATATCTGTCTGTGGCATTCGTCACAGAAGTAGATGTAGGTAAAATGGCCTTCATATGGGAGCTTATGGACTGCAGCTCCGGGTAGGATCCGATGAACGTAATCGGTCATTGATGGTGGAACCACCATATCATCCATCCCCTGTTTGAATGAAAATATGAGATCAAAAGGGAGAGGCAAACCAAACTACAATTCACAGCTCCAATTAGGATTTGAAAAACAAT

Coding sequence (CDS)

ATGCTAAGTAGGGGAGGTTTAGTCTCATCCTATATTTCTTCTTCTTTGAAATGCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATACCAGAATCTTCACCAGTGAAGATGTTTTGGAGTACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCGGCTTTAGCCCGACGTGCTGGATTCGACGATCGTCTTGTTCATGGGATGCTTGTTGCATCAATGTTCCCTCATGTCATTTCCTCTCATTTTCCAGGAGCCATCTATGTGTCACAAAGCTTGAATTTTAAGTTACCAGTCTACGTCGGAGAGACGATCATGGTTCGGGTTGAAGCAATCGACTTGAGAGAAATTAAGAAGAGATACCTGGCGAAATTCCAAACGAAATGCTTGAGGAATGGCGACGAGCTTGTTCTGGATGGTGAGGCAAGAGCCATCTTACCTTCTGGTTTTTGTTCCACAGATGGTTAG

Protein sequence

MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG
Homology
[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-18
Identity = 52/131 (39.69%), Postives = 75/131 (57.25%), Query Frame = 0 

Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPHV 86
           L+VG     ++ F + +V  ++ +S D NPLH D A A    F+  +VHGML+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 87  ISSHFP--GAIYVSQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQTKCLRNGDEL 146
           +    P  G+IY+ QSL+FKLPV+VG+ +   VE   LRE K   +A   T+    G  L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 147 VLDGEARAILP 156
            + GEA   LP
Sbjct: 126 AVTGEAVVKLP 134

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.1e-16
Identity = 45/118 (38.14%), Postives = 71/118 (60.17%), Query Frame = 0 

Query: 40  TSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPHVISSHF--PGAIYV 99
           +S DV+ +++++ D NP+H     A R  F  R+ HG+  A +   V+ +    PGA+Y+
Sbjct: 26  SSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAVLGTRLPGPGAVYI 85

Query: 100 SQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQTKCLRNGDELVLDGEARAILP 156
           SQ+LNF+ PV +G+ + V+VE  +L  I +R  A+    C   GDE+VLDGEA   +P
Sbjct: 86  SQTLNFRAPVRIGDIVEVKVEVAEL--IPERRRARLACTC-SVGDEVVLDGEALVKVP 140

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.4e-14
Identity = 44/132 (33.33%), Postives = 72/132 (54.55%), Query Frame = 0 

Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPHV 86
           +K G   S  +  +  D+  ++ +S D+NP H +   A+   F  R+ HGML A     V
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQTKCLRNGDEL 146
           + +    PGAIYV+QSL FK PV +G+T+   V    L  + ++    F+T C   G ++
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGL--VPEKKFVTFRTTCTVAG-KV 133

Query: 147 VLDGEARAILPS 157
           V++GEA  ++P+
Sbjct: 134 VIEGEATVMVPA 142

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 39/132 (29.55%), Postives = 72/132 (54.55%), Query Frame = 0 

Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLVHGMLVASMFPHV 86
           +KVGD     R FT  DV  +S+++ D NPLH +   A+   F + +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQTKC-LRNGDE 146
           + +    PG +++SQ ++F  P+Y+GE ++   E   L    KR++A     C +    +
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKK 155

Query: 147 LVLDGEARAILP 156
            V++G  + ++P
Sbjct: 156 TVMEGWVKVMVP 163

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match: Q5AV07 (Fatty acid synthase beta subunit aflB OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=atnM PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 4.1e-04
Identity = 30/82 (36.59%), Postives = 45/82 (54.88%), Query Frame = 0 

Query: 40   TSEDVLEYSKVSHDSNPLHFDSALARRAGFDD-RLVHGMLVASMFPHVISSHFPG---AI 99
            TS D  +Y+  S DSNP+H   A AR AG ++ R++HGM  + +   V+  H  G     
Sbjct: 1609 TSSD--QYAAASGDSNPIHLSRAFARYAGHNEGRVIHGMQTSGLVRGVVELHAAGNDPRR 1668

Query: 100  YVSQSLNFKLPVYVGETIMVRV 118
              + S +FK  V  GET++V +
Sbjct: 1669 MKAWSASFKGKVSPGETLLVDI 1688

[Close]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match: XP_023512018.1 (uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318 bits (816), Expect = 1.65e-109
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163

[Close]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match: XP_022985629.1 (uncharacterized protein LOC111483634 [Cucurbita maxima])

HSP 1 Score: 311 bits (796), Expect = 1.85e-106
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFP VISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPS FCSTDG
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTDG 163

[Close]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match: KAG7010611.1 ((R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315 bits (806), Expect = 3.13e-106
Identity = 160/163 (98.16%), Postives = 163/163 (100.00%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 17  MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 76

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 77  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 136

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKR+LAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG
Sbjct: 137 DLREIKKRHLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 179

[Close]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match: KAG6570765.1 (Adenylate kinase isoenzyme 6-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315 bits (806), Expect = 5.04e-105
Identity = 160/163 (98.16%), Postives = 163/163 (100.00%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 202 MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 261

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 262 SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 321

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKR+LAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG
Sbjct: 322 DLREIKKRHLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 364

[Close]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match: XP_022943531.1 (uncharacterized protein LOC111448279 [Cucurbita moschata])

HSP 1 Score: 296 bits (757), Expect = 1.42e-100
Identity = 155/163 (95.09%), Postives = 157/163 (96.32%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SS ISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSCISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKRYL KFQTKCLRNGDELVLDGEARAILPS    TDG
Sbjct: 121 DLREIKKRYLVKFQTKCLRNGDELVLDGEARAILPS----TDG 159

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 311 bits (796), Expect = 8.95e-107
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFP VISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPS FCSTDG
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTDG 163

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match: A0A6J1FRZ2 (uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC111448279 PE=4 SV=1)

HSP 1 Score: 296 bits (757), Expect = 6.90e-101
Identity = 155/163 (95.09%), Postives = 157/163 (96.32%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRGGL+SS ISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSCISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETI+VRVEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163
           DLREIKKRYL KFQTKCLRNGDELVLDGEARAILPS    TDG
Sbjct: 121 DLREIKKRYLVKFQTKCLRNGDELVLDGEARAILPS----TDG 159

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 259 bits (662), Expect = 2.31e-86
Identity = 136/162 (83.95%), Postives = 150/162 (92.59%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG L S++ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRG-LFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
             LARRAGF+  LVHG+LVASMFPH+ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 162
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP+ F ST+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 258 bits (659), Expect = 6.62e-86
Identity = 135/162 (83.33%), Postives = 150/162 (92.59%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLS+G L S+YISSSLKCFSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSKG-LFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
           S LA+RAGF+  LVHG+LVASMFPH+ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SKLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 162
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP  F ST+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 259 bits (662), Expect = 1.02e-85
Identity = 136/162 (83.95%), Postives = 150/162 (92.59%), Query Frame = 0 

Query: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG L S++ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 45  MLSRG-LFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 104

Query: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120
             LARRAGF+  LVHG+LVASMFPH+ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 105 PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 164

Query: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 162
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP+ F ST+
Sbjct: 165 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]
BLAST of Cp4.1LG16g05470 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 3.4e-46
Identity = 88/143 (61.54%), Postives = 117/143 (81.82%), Query Frame = 0 

Query: 16  LKCFSS-STSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGFDDRLV 75
           L+ FSS ++  +LKVGD+L  TR+F+SED+  Y++VSHD NPLHFD   AR+AGF++RLV
Sbjct: 14  LRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDPESARKAGFENRLV 73

Query: 76  HGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAIDLREIKKRYLAKFQ 135
           HGMLV+SMFP +IS+HFPGA+YVSQSL+F+ PVY+G+ I+  V+AI LRE K +Y+ KF 
Sbjct: 74  HGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIALRETKNKYIVKFS 133

Query: 136 TKCLRNGDEL-VLDGEARAILPS 157
           TKC +N +EL V+DGEA AILP+
Sbjct: 134 TKCFKNHNELVVIDGEATAILPN 156

The following BLAST results are available for this feature:
BLAST of Cp4.1LG16g05470 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O324723.8e-1839.69(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1[more]
C5AZ742.1e-1638.143-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ... [more]
Q2RQ364.4e-1433.33(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A... [more]
P863971.3e-1329.55Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ... [more]
Q5AV074.1e-0436.59Fatty acid synthase beta subunit aflB OS=Emericella nidulans (strain FGSC A4 / A... [more]
BLAST of Cp4.1LG16g05470 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023512018.11.65e-109100.00uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo][more]
XP_022985629.11.85e-10697.55uncharacterized protein LOC111483634 [Cucurbita maxima][more]
KAG7010611.13.13e-10698.16(R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyros... [more]
KAG6570765.15.04e-10598.16Adenylate kinase isoenzyme 6-like protein, partial [Cucurbita argyrosperma subsp... [more]
XP_022943531.11.42e-10095.09uncharacterized protein LOC111448279 [Cucurbita moschata][more]
BLAST of Cp4.1LG16g05470 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JDU38.95e-10797.55uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634... [more]
A0A6J1FRZ26.90e-10195.09uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC1114482... [more]
A0A5A7T1472.31e-8683.95(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0KCN36.62e-8683.33MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P... [more]
A0A1S3CLU31.02e-8583.95(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV... [more]
BLAST of Cp4.1LG16g05470 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G60335.13.4e-4661.54Thioesterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002539MaoC-like dehydratase domainPFAMPF01575MaoC_dehydratascoord: 38..128
e-value: 2.4E-18
score: 65.9
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 16..157
e-value: 1.1E-36
score: 127.4
NoneNo IPR availablePANTHERPTHR43437HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATEDcoord: 9..156
NoneNo IPR availablePANTHERPTHR43437:SF3HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATEDcoord: 9..156
NoneNo IPR availableCDDcd03449R_hydratasecoord: 27..154
e-value: 8.8006E-47
score: 146.921
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 27..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g05470.1Cp4.1LG16g05470.1mRNA