Cp4.1LG16g05250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g05250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionruBisCO large subunit-binding protein subunit alpha-like
LocationCp4.1LG16: 6145271 .. 6149064 (-)
RNA-Seq ExpressionCp4.1LG16g05250
SyntenyCp4.1LG16g05250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGTATGTATGGCCTCTTCCATCCTTAGTGGTGTGGTTATTGTTATTATTATTATTTTGATTTATTTGATTTAAAATAAGTAATGGATCAGATAACTATTGGGCTGAAATGGATTTTACCGAAGCTAACCCAAAGCCCAATAGGCCCATATTTCGACTATTGCGATTCAAGGCGCACGTTAGCATGATGAAATCTTGAAAGCTTAAACAGCGCCATGAGTGCGCACTGCACTGAAGATTTCAAAAATCGCATTTGGGCCGCAACGTAACTAAACAACAACAATCCTGAAATAGGCTTCAAGGGTTCTGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCTTCTGGAACTTCCAATCCCACAATAAAAACCGAGCCCTAAACTCATTCAGCGCTTTGCTTACCCACCTTATCCCAAAATCCAGCTAGCTCTACCAAAACTAAAACCCTCCGAGCTACGCTGCCTGAAAATGGCGTCTGCTAATGCAATTTCTTCTGCATCGATTCTATGTCCTTCCCAGAAGGTACTACCTTGATTTCTCATTTCTTTGTTGTTATTCTACTGCAATTCCTCCCCTTACCTTGTAATCTTTACAATTCTTCGCTTTCCTTCATCTTTGAAAATGATGATTTGTAGATTATAGTCGTTGCGATTCTATACTGTTGTGTTTGCGCTGTATGAGAATCATGTGAGCTACGTAATTGGCGTTCTATGTAGAAAGTTTGTGGAGTTTGTTATCATAGATGAGCTGAGAGTGGGTATTTCATTCTTGTAAAATGTGTTGGTGGTGGAATTGTGAAGGAGAAACTGATAATGCTAATGTAGAATTTCCATTAACTTACGCTGCATTTATCTAACGTTGCAGAGCTTGAGAAAGGCGAATCAAACACAGAACAACAGGCTACATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCTAAGGAGATAGCGTTTGACCAAAGATCTAGGAGCGCACTTCAGTCTGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGTAACTTCTTGGTTCTTTTGATTTGTCAATTACTCAGTATAGAAATTCGTTTTACTTTATGGAAATAGATTCATTTCATGTTTTGTTACTCCATCGGATGGATTTTGAGAATTTTGGGTTCTTAACTAAATGTTCAACTTGTTGTGACCTTTGTATTTGAAGGGAGAAATGTGGTGCTGGATGAGTTCGGCAGTCCTAAGGTTGTAAACGATGGTGTGACAATTGCTCGGGCAATTGAGTTGCCTGATCCCATGGAAAATGCTGGTGCAGCTCTAATTAGAGAGGTTGGTCCTCCTTTACACTCTGATCTTCCTGTTATATTTGATCTGTTGTGATGCAATAATTTATCATCCATCAGCTATTGAAATCCTGTAAGCATGCTATTTGTCATGAAGGTTGCAAGTAAAACTAATGATTCTGCTGGTGATGGGACGACAACTGCCTCAGTCCTCGCTAGGGAAATTATCAAGCTAGGCCTGCTTAATGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAGAGGAATTGACAAGACCGTGCAAGGATTGATCCAAGAGCTTGAGAACAAGGCTAGGGCTATAGAAGGCAGAGATGATATCAAAGGTATTGCGTTCTGCTGGGAATTAGTAGAAAACAACCAAAATATGAAATACAAAAGTTTACTAGAGGAGAAAAATCAGTACCCACCAGAAAGAAATTGAATGTAAAAGTTTTTTATAATCACAATATTTTTTCATCAATTGATGATCCTTGTTTTGTTTTAATTCTGTGTAGCTGTTGCTTCTATTTCTGCTGGAAATGACGAGCTTATAGGGATGATGATCGCTGATGCCATTGAAAAAGTTGGGCCTGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGGTGTGTAGTTTTGCTCTTTACTGAAGAGTATCTTTCTTAATCCAAATCCAAGTCATTCATGCACGTTATAACTCTACTCTATGTCTTCATCTTTTTCATGTTGCAGATTGACAGAGGCTATATTTCTCCTCAGTTCGTCACAAACCCTGAAAAATTAATTGCTGAATTTGAGAATGCACGAGTATTAGTTACAGACCAGAAAATTTCGGCCATAAAGGATATAATCCCTATATTGGAGAAAACCACGCAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGATGTTACTGGTGAGGCTTTGGCTACCCTTGTTGTGAACAAGTTGCGGGGGATTCTAAATGTTGCTGCCATTAAAGCTCCGGGCTTTGGAGAAAGGAGAAAGGCGATGCTTCAAGACATTGCCATTTTGACAGGTAGTCTATTTAACGAAAGGTTCGCAAGGAGGAGTTAATTTTATAAATTTGATCTGCTTTTATGTTGATTTGAGTAGAATGGCTAACGTTTTTCTTGTTACTTTATTATTGTTTTAGGTGCTGAGTTTCAAGCCAATGATCTTGGATTGCTAGTAGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCCAAGGACACTACCACCATCATTGCTGACGCTGCTTCAAAAGACGAGCTACAAGCCAGAATTGCACAGCTAAAGAAGGAATTGGCTGAGACAGATTCTGTGTATGACACAGAGAAACTCGCTGAAAGAATTGCCAAACTATCTGGTGGTGTTGCTGTCATTAAAGTAGGAGCTGCAACAGAGACTGAACTTGAGGACCGTAAGCTCCGTATTGAAGATGCAAAGAATGCAACTTTCGCTGCCATAGAGGAAGGCATTGTACCTGGTGGTGGTGCTGCACTGGTTCACCTCTCAACTCTTGTCCCTGCAATCAAGGACAAGCTCGAAGATGCCGAAGAGAAGCTCGGTGCCGATATTGTCCAAAAGGTAACCGAACTCCGTAGTGCAATCCTGGAACAACTACTGGGTGCTGCATCTGGTCTTTTGTTATTGACTTGAATGTTTTTGTACACAGGCGCTGGTAGCACCGGCATCCTTAATTGCCCAAAATGCTGGAATTGAAGGGGAAGTAGTGGTGGAGAAGATAAAGTCAAGTGAATGGGAAATTGGTTACAACGCAATGACAGACAAGTACGAGAATCTAGTAGAGGCCGGCGTTATTGACCCAGCCAAGGTGACCAGATGTGCCTTGCAGAATGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCTATTGTAGTTGAAAAGCCCAAGCCCAAGGCACCCACCGCTGCCCCACAAGGCCTTACTATTTAAATTTCTAAGAATTTTCATATCTTCCCTTTGTAGGATTTTCAGATATCACGAACGTGGTGGGTAGTCTGGCGGCGCGCATTCATTGGAGGAAAAGGCAAAACAATAAGGAGACGCAACTCCGTGTGAATGCACCTTTTTTATGTAAGGTTCTCTGGAAACTTTCATTTAGATATATTGTAAGATTACTGAATGAATTGGATTAACTTTATGAGTTTTCATTGAAAATAAATAATTTTGATGAATTGGGTCAGTGGTGGGGCACCCCTTTAGATGATTTCATGTGTTAAAATGACCCTTGAGGGTGGGGATTTGACAGAAACAGACAGCACCTCTGTTGTTCTTTTTGTGATATCCTTCCATTAATTGGCTTTTTAAGATGGAATCTACAATGAAATCCACTGTTCCTCAGGGGAAATAATAATGAATGTAGGGTCCGAATCGTTACACGTGTCGGCCTA

mRNA sequence

ATGTATCGCTTTGCTTACCCACCTTATCCCAAAATCCAGCTAGCTCTACCAAAACTAAAACCCTCCGAGCTACGCTGCCTGAAAATGGCGTCTGCTAATGCAATTTCTTCTGCATCGATTCTATGTCCTTCCCAGAAGAGCTTGAGAAAGGCGAATCAAACACAGAACAACAGGCTACATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCTAAGGAGATAGCGTTTGACCAAAGATCTAGGAGCGCACTTCAGTCTGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGGAGAAATGTGGTGCTGGATGAGTTCGGCAGTCCTAAGGTTGTAAACGATGGTGTGACAATTGCTCGGGCAATTGAGTTGCCTGATCCCATGGAAAATGCTGGTGCAGCTCTAATTAGAGAGGTTGCAAGTAAAACTAATGATTCTGCTGGTGATGGGACGACAACTGCCTCAGTCCTCGCTAGGGAAATTATCAAGCTAGGCCTGCTTAATGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAGAGGAATTGACAAGACCGTGCAAGGATTGATCCAAGAGCTTGAGAACAAGGCTAGGGCTATAGAAGGCAGAGATGATATCAAAGCTGTTGCTTCTATTTCTGCTGGAAATGACGAGCTTATAGGGATGATGATCGCTGATGCCATTGAAAAAGTTGGGCCTGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGATTGACAGAGGCTATATTTCTCCTCAGTTCGTCACAAACCCTGAAAAATTAATTGCTGAATTTGAGAATGCACGAGTATTAGTTACAGACCAGAAAATTTCGGCCATAAAGGATATAATCCCTATATTGGAGAAAACCACGCAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGATGTTACTGGTGAGGCTTTGGCTACCCTTGTTGTGAACAAGTTGCGGGGGATTCTAAATGTTGCTGCCATTAAAGCTCCGGGCTTTGGAGAAAGGAGAAAGGCGATGCTTCAAGACATTGCCATTTTGACAGGTGCTGAGTTTCAAGCCAATGATCTTGGATTGCTAGTAGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCCAAGGACACTACCACCATCATTGCTGACGCTGCTTCAAAAGACGAGCTACAAGCCAGAATTGCACAGCTAAAGAAGGAATTGGCTGAGACAGATTCTGTGTATGACACAGAGAAACTCGCTGAAAGAATTGCCAAACTATCTGGTGGTGTTGCTGTCATTAAAGTAGGAGCTGCAACAGAGACTGAACTTGAGGACCGTAAGCTCCGTATTGAAGATGCAAAGAATGCAACTTTCGCTGCCATAGAGGAAGGCATTGTACCTGGTGGTGGTGCTGCACTGGTTCACCTCTCAACTCTTGTCCCTGCAATCAAGGACAAGCTCGAAGATGCCGAAGAGAAGCTCGGTGCCGATATTGTCCAAAAGGCGCTGGTAGCACCGGCATCCTTAATTGCCCAAAATGCTGGAATTGAAGGGGAAGTAGTGGTGGAGAAGATAAAGTCAAGTGAATGGGAAATTGGTTACAACGCAATGACAGACAAGTACGAGAATCTAGTAGAGGCCGGCGTTATTGACCCAGCCAAGGTGACCAGATGTGCCTTGCAGAATGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCTATTGTAGTTGAAAAGCCCAAGCCCAAGGCACCCACCGCTGCCCCACAAGGCCTTACTATTTAAATTTCTAAGAATTTTCATATCTTCCCTTTGTAGGATTTTCAGATATCACGAACGTGGTGGGTAGTCTGGCGGCGCGCATTCATTGGAGGAAAAGGCAAAACAATAAGGAGACGCAACTCCGTGTGAATGCACCTTTTTTATGTAAGGTTCTCTGGAAACTTTCATTTAGATATATTGTAAGATTACTGAATGAATTGGATTAACTTTATGAGTTTTCATTGAAAATAAATAATTTTGATGAATTGGGTCAGTGGTGGGGCACCCCTTTAGATGATTTCATGTGTTAAAATGACCCTTGAGGGTGGGGATTTGACAGAAACAGACAGCACCTCTGTTGTTCTTTTTGTGATATCCTTCCATTAATTGGCTTTTTAAGATGGAATCTACAATGAAATCCACTGTTCCTCAGGGGAAATAATAATGAATGTAGGGTCCGAATCGTTACACGTGTCGGCCTA

Coding sequence (CDS)

ATGTATCGCTTTGCTTACCCACCTTATCCCAAAATCCAGCTAGCTCTACCAAAACTAAAACCCTCCGAGCTACGCTGCCTGAAAATGGCGTCTGCTAATGCAATTTCTTCTGCATCGATTCTATGTCCTTCCCAGAAGAGCTTGAGAAAGGCGAATCAAACACAGAACAACAGGCTACATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCTAAGGAGATAGCGTTTGACCAAAGATCTAGGAGCGCACTTCAGTCTGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGGAGAAATGTGGTGCTGGATGAGTTCGGCAGTCCTAAGGTTGTAAACGATGGTGTGACAATTGCTCGGGCAATTGAGTTGCCTGATCCCATGGAAAATGCTGGTGCAGCTCTAATTAGAGAGGTTGCAAGTAAAACTAATGATTCTGCTGGTGATGGGACGACAACTGCCTCAGTCCTCGCTAGGGAAATTATCAAGCTAGGCCTGCTTAATGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAGAGGAATTGACAAGACCGTGCAAGGATTGATCCAAGAGCTTGAGAACAAGGCTAGGGCTATAGAAGGCAGAGATGATATCAAAGCTGTTGCTTCTATTTCTGCTGGAAATGACGAGCTTATAGGGATGATGATCGCTGATGCCATTGAAAAAGTTGGGCCTGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGATTGACAGAGGCTATATTTCTCCTCAGTTCGTCACAAACCCTGAAAAATTAATTGCTGAATTTGAGAATGCACGAGTATTAGTTACAGACCAGAAAATTTCGGCCATAAAGGATATAATCCCTATATTGGAGAAAACCACGCAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGATGTTACTGGTGAGGCTTTGGCTACCCTTGTTGTGAACAAGTTGCGGGGGATTCTAAATGTTGCTGCCATTAAAGCTCCGGGCTTTGGAGAAAGGAGAAAGGCGATGCTTCAAGACATTGCCATTTTGACAGGTGCTGAGTTTCAAGCCAATGATCTTGGATTGCTAGTAGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCCAAGGACACTACCACCATCATTGCTGACGCTGCTTCAAAAGACGAGCTACAAGCCAGAATTGCACAGCTAAAGAAGGAATTGGCTGAGACAGATTCTGTGTATGACACAGAGAAACTCGCTGAAAGAATTGCCAAACTATCTGGTGGTGTTGCTGTCATTAAAGTAGGAGCTGCAACAGAGACTGAACTTGAGGACCGTAAGCTCCGTATTGAAGATGCAAAGAATGCAACTTTCGCTGCCATAGAGGAAGGCATTGTACCTGGTGGTGGTGCTGCACTGGTTCACCTCTCAACTCTTGTCCCTGCAATCAAGGACAAGCTCGAAGATGCCGAAGAGAAGCTCGGTGCCGATATTGTCCAAAAGGCGCTGGTAGCACCGGCATCCTTAATTGCCCAAAATGCTGGAATTGAAGGGGAAGTAGTGGTGGAGAAGATAAAGTCAAGTGAATGGGAAATTGGTTACAACGCAATGACAGACAAGTACGAGAATCTAGTAGAGGCCGGCGTTATTGACCCAGCCAAGGTGACCAGATGTGCCTTGCAGAATGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCTATTGTAGTTGAAAAGCCCAAGCCCAAGGCACCCACCGCTGCCCCACAAGGCCTTACTATTTAA

Protein sequence

MYRFAYPPYPKIQLALPKLKPSELRCLKMASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Homology
BLAST of Cp4.1LG16g05250 vs. ExPASy Swiss-Prot
Match: P08926 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 936.4 bits (2419), Expect = 1.6e-271
Identity = 508/588 (86.39%), Postives = 551/588 (93.71%), Query Frame = 0

Query: 29  MASANAISSASIL----CPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSR 88
           MAS NA+SS SIL      +Q SL K    Q+ R+++RQ  +RFVV+A AK+IAFDQ SR
Sbjct: 1   MASTNALSSTSILRSPTNQAQTSLSK-KVKQHGRVNFRQKPNRFVVKAAAKDIAFDQHSR 60

Query: 89  SALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALI 148
           SA+Q+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALI
Sbjct: 61  SAMQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALI 120

Query: 149 REVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENK 208
           REVASKTNDSAGDGTTTAS+LAREIIKLGLLNVTSGANPVS+K+GIDKTV  L++ELE  
Sbjct: 121 REVASKTNDSAGDGTTTASILAREIIKLGLLNVTSGANPVSIKKGIDKTVAALVEELEKL 180

Query: 209 ARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMA 268
           AR ++G DDIKAVA+ISAGNDELIG MIA+AI+KVGPDGVLSIESS+SFETTVEVEEGM 
Sbjct: 181 ARPVKGGDDIKAVATISAGNDELIGKMIAEAIDKVGPDGVLSIESSNSFETTVEVEEGME 240

Query: 269 IDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDV 328
           IDRGYISPQFVTNPEK I EFENARVL+TDQKISAIKDIIP+LEKTTQLRAPLLII+ED+
Sbjct: 241 IDRGYISPQFVTNPEKSIVEFENARVLITDQKISAIKDIIPLLEKTTQLRAPLLIISEDI 300

Query: 329 TGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSI 388
           TGEALATLVVNKLRGILNVAAIKAPGFGERRKA+LQDIAILTGAEFQA+DLGLLVENT+I
Sbjct: 301 TGEALATLVVNKLRGILNVAAIKAPGFGERRKALLQDIAILTGAEFQASDLGLLVENTTI 360

Query: 389 EQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSG 448
           EQLGLARKVTISKD+TTIIADAASKDELQ+R+AQLKKEL+ETDS+YD+EKLAERIAKLSG
Sbjct: 361 EQLGLARKVTISKDSTTIIADAASKDELQSRVAQLKKELSETDSIYDSEKLAERIAKLSG 420

Query: 449 GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLE 508
           GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG ALVHLS  VPAIK+KLE
Sbjct: 421 GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSGYVPAIKEKLE 480

Query: 509 DAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAG 568
           DA+E+LGADIVQKALVAPA+LIAQNAGIEGEVVVEKIK+ EWE+GYNAMTD YENLVE+G
Sbjct: 481 DADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIKNGEWEVGYNAMTDTYENLVESG 540

Query: 569 VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-TAAPQGLTI 612
           VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKA   AAPQGLTI
Sbjct: 541 VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAAVAAAPQGLTI 587

BLAST of Cp4.1LG16g05250 vs. ExPASy Swiss-Prot
Match: P21238 (Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60A1 PE=1 SV=2)

HSP 1 Score: 924.5 bits (2388), Expect = 6.4e-268
Identity = 500/586 (85.32%), Postives = 546/586 (93.17%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKS-LRKANQTQNNRLHY-RQAGSRFVVRATAKEIAFDQRSRSA 88
           MASANA+SSAS+LC S++S L   NQ Q  R+ Y ++   RF VRA  KEIAFDQ SR+A
Sbjct: 1   MASANALSSASVLCSSRQSKLGGGNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAA 60

Query: 89  LQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIRE 148
           LQ+GIDKLA+ VGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELP+ MENAGAALIRE
Sbjct: 61  LQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIRE 120

Query: 149 VASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKAR 208
           VASKTNDSAGDGTTTAS+LAREIIK GLL+VTSGANPVSLKRGIDKTVQGLI+EL+ KAR
Sbjct: 121 VASKTNDSAGDGTTTASILAREIIKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKAR 180

Query: 209 AIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAID 268
            ++GRDDI+AVASISAGND+LIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGM ID
Sbjct: 181 PVKGRDDIRAVASISAGNDDLIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEID 240

Query: 269 RGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTG 328
           RGYISPQFVTNPEKL+AEFENARVL+TDQKI+AIKDIIPILEKTTQLRAPLLIIAEDVTG
Sbjct: 241 RGYISPQFVTNPEKLLAEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTG 300

Query: 329 EALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQ 388
           EALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAILTGAE+ A D+ LLVEN +I+Q
Sbjct: 301 EALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQ 360

Query: 389 LGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGV 448
           LG+ARKVTISKD+TT+IADAASKDELQARIAQLKKEL ETDSVYD+EKLAERIAKLSGGV
Sbjct: 361 LGIARKVTISKDSTTLIADAASKDELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGV 420

Query: 449 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDA 508
           AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST++PAIK+  EDA
Sbjct: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDA 480

Query: 509 EEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVI 568
           +E+LGADIVQKAL++PA+LIAQNAG+EGEVVVEKI  S+WE GYNAMTD YENL EAGVI
Sbjct: 481 DERLGADIVQKALLSPAALIAQNAGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVI 540

Query: 569 DPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-TAAPQGLTI 612
           DPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 586

BLAST of Cp4.1LG16g05250 vs. ExPASy Swiss-Prot
Match: P21239 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS=Brassica napus OX=3708 PE=2 SV=2)

HSP 1 Score: 912.9 bits (2358), Expect = 1.9e-264
Identity = 487/546 (89.19%), Postives = 524/546 (95.97%), Query Frame = 0

Query: 67  RFVVRATAKEIAFDQRSRSALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTI 126
           RF VRA  KEI+FDQ SR+ALQ+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTI
Sbjct: 1   RFSVRANVKEISFDQSSRAALQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTI 60

Query: 127 ARAIELPDPMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSL 186
           ARAIELPD MENAGAALIREVASKTNDSAGDGTTTASVLAREIIK GLL+VTSGANPVSL
Sbjct: 61  ARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKHGLLSVTSGANPVSL 120

Query: 187 KRGIDKTVQGLIQELENKARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLS 246
           KRGIDKTVQ LI+ELE +AR ++G  DIKAVA+ISAGNDEL+G MIADAI+KVGPDGVLS
Sbjct: 121 KRGIDKTVQALIEELEKRARPVKGGSDIKAVATISAGNDELVGTMIADAIDKVGPDGVLS 180

Query: 247 IESSSSFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPI 306
           IESSSSFETTVEVEEGM IDRGYISPQFVTNPEKL+ EFENARVL+TDQKI+AIKDIIPI
Sbjct: 181 IESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLVEFENARVLITDQKITAIKDIIPI 240

Query: 307 LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILT 366
           LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAILT
Sbjct: 241 LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILT 300

Query: 367 GAEFQANDLGLLVENTSIEQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAET 426
           GAE+QA D+GLLVENT+I+QLG+ARKVTISKD+TT+IADAASKDELQARI+QLKKEL+ET
Sbjct: 301 GAEYQALDMGLLVENTTIDQLGIARKVTISKDSTTLIADAASKDELQARISQLKKELSET 360

Query: 427 DSVYDTEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG 486
           DSVYD+EKLAERIAKL+GGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG
Sbjct: 361 DSVYDSEKLAERIAKLAGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG 420

Query: 487 AALVHLSTLVPAIKDKLEDAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEW 546
           A LVHLST++PAIK+KLEDA+E+LGADIVQKALVAPA+LIAQNAGIEGEVVVEKI  SEW
Sbjct: 421 ATLVHLSTVIPAIKEKLEDADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIMFSEW 480

Query: 547 EIGYNAMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAA- 606
           EIGYNAMTD YENL+EAGVIDPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAPTAA 
Sbjct: 481 EIGYNAMTDTYENLLEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPTAAP 540

Query: 607 PQGLTI 612
           PQGL +
Sbjct: 541 PQGLMV 546

BLAST of Cp4.1LG16g05250 vs. ExPASy Swiss-Prot
Match: P34794 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 1.2e-258
Identity = 491/585 (83.93%), Postives = 533/585 (91.11%), Query Frame = 0

Query: 29  MASANAISSASILCPS-QKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSAL 88
           MA+ANA+SS S+LC S Q  L   +Q +  R+ YR+A  RF +RA  KEIAFDQ SR+AL
Sbjct: 1   MATANALSSPSVLCSSRQGKLSGGSQQKGQRVSYRKANRRFSLRANVKEIAFDQSSRAAL 60

Query: 89  QSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREV 148
           Q+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREV
Sbjct: 61  QAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREV 120

Query: 149 ASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARA 208
           ASKTNDSAGDGTTTASVLAREIIK GLL+VTSGANPVSLKRGIDKTVQ LI+ELE ++R 
Sbjct: 121 ASKTNDSAGDGTTTASVLAREIIKHGLLSVTSGANPVSLKRGIDKTVQALIEELEKRSRP 180

Query: 209 IEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDR 268
           ++G  DIKAVA+ISAGNDELIG MIADAI+KVGPDGV  IESSSSFETTVEVEEGM IDR
Sbjct: 181 VKGGRDIKAVATISAGNDELIGAMIADAIDKVGPDGVSPIESSSSFETTVEVEEGMEIDR 240

Query: 269 GYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGE 328
           GYISPQFVTNPEKL+ EFENARVL+TDQKI+AIKDIIPILEKTTQLRAPLLIIAEDVTGE
Sbjct: 241 GYISPQFVTNPEKLLVEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTGE 300

Query: 329 ALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQL 388
           ALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAILT     A D+GLLVENT+I+QL
Sbjct: 301 ALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTEPS-TALDMGLLVENTTIDQL 360

Query: 389 GLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVA 448
           G+ARKVTISKD+TT+IADAASK ELQARI+QLKKE  ETDSVYD+EKLAERIAKLSGGVA
Sbjct: 361 GIARKVTISKDSTTLIADAASKAELQARISQLKKESFETDSVYDSEKLAERIAKLSGGVA 420

Query: 449 VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAE 508
           VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGA LVHLST++PAIK+  EDA+
Sbjct: 421 VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGATLVHLSTVIPAIKETFEDAD 480

Query: 509 EKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVID 568
            +LGADIVQKALVA  SLIAQNAGIEGEVVVEKI  SEWE+GYNAMTD YENL+EAGVID
Sbjct: 481 VRLGADIVQKALVA-QSLIAQNAGIEGEVVVEKIMFSEWELGYNAMTDTYENLLEAGVID 540

Query: 569 PAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-TAAPQGLTI 612
           PAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 PAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 583

BLAST of Cp4.1LG16g05250 vs. ExPASy Swiss-Prot
Match: P08824 (RuBisCO large subunit-binding protein subunit alpha (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 1.1e-248
Identity = 457/495 (92.32%), Postives = 486/495 (98.18%), Query Frame = 0

Query: 84  RSALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAAL 143
           R+ALQSGIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVN+GVTIARAIELPDPMENAGAAL
Sbjct: 1   RTALQSGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNEGVTIARAIELPDPMENAGAAL 60

Query: 144 IREVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELEN 203
           IREVASKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVS+KRGIDKTVQGLI+ELE 
Sbjct: 61  IREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSIKRGIDKTVQGLIEELEK 120

Query: 204 KARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGM 263
           KAR ++GRDDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGM
Sbjct: 121 KARPVKGRDDIKAVASISAGNDELIGTMIADAIDKVGPDGVLSIESSSSFETTVEVEEGM 180

Query: 264 AIDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAED 323
            IDRGYISPQFVTNPEKLI EFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAED
Sbjct: 181 EIDRGYISPQFVTNPEKLICEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAED 240

Query: 324 VTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTS 383
           VTGEALATLVVNK+RGILNVAAIKAPGFGERRKA+LQDIAILTGAEFQA+DLGLLVENTS
Sbjct: 241 VTGEALATLVVNKMRGILNVAAIKAPGFGERRKALLQDIAILTGAEFQASDLGLLVENTS 300

Query: 384 IEQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLS 443
           +EQLG+ARKVTI+KD+TT+IADAASKDELQARIAQLK+ELAETDSVYD+EKLAERIAKLS
Sbjct: 301 VEQLGIARKVTITKDSTTLIADAASKDELQARIAQLKRELAETDSVYDSEKLAERIAKLS 360

Query: 444 GGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKL 503
           GGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST+VPAI  + 
Sbjct: 361 GGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTVVPAINGED 420

Query: 504 EDAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEA 563
           +DA+E+LGADI+QKALVAPASLIAQNAGIEGEVVVEK+K+ EWEIGYNAMTDKYENLVEA
Sbjct: 421 KDADERLGADILQKALVAPASLIAQNAGIEGEVVVEKVKAREWEIGYNAMTDKYENLVEA 480

Query: 564 GVIDPAKVTRCALQN 579
           GVIDPAKVTRCALQN
Sbjct: 481 GVIDPAKVTRCALQN 495

BLAST of Cp4.1LG16g05250 vs. NCBI nr
Match: XP_023511889.1 (ruBisCO large subunit-binding protein subunit alpha [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080 bits (2793), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ
Sbjct: 1   MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. NCBI nr
Match: XP_022985565.1 (ruBisCO large subunit-binding protein subunit alpha-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 579/583 (99.31%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFEN RVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENTRVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. NCBI nr
Match: KAG6570801.1 (hypothetical protein SDJN03_29716, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1068 bits (2763), Expect = 0.0
Identity = 578/583 (99.14%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. NCBI nr
Match: XP_022943380.1 (ruBisCO large subunit-binding protein subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 577/583 (98.97%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTV 583

BLAST of Cp4.1LG16g05250 vs. NCBI nr
Match: KAG7010643.1 (hypothetical protein SDJN02_27438 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 577/583 (98.97%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKL+ERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLSERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. ExPASy TrEMBL
Match: A0A6J1JBN6 (ruBisCO large subunit-binding protein subunit alpha-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483598 PE=3 SV=1)

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 579/583 (99.31%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFEN RVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENTRVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. ExPASy TrEMBL
Match: A0A6J1FXR2 (ruBisCO large subunit-binding protein subunit alpha-like OS=Cucurbita moschata OX=3662 GN=LOC111448162 PE=3 SV=1)

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 577/583 (98.97%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANAISSASILC SQKSLRKANQTQNNRL+YRQAGSRFVVRATAKEIAFDQ SRSALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTV 583

BLAST of Cp4.1LG16g05250 vs. ExPASy TrEMBL
Match: A0A5D3DFR6 (RuBisCO large subunit-binding protein subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold173G00190 PE=3 SV=1)

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 567/583 (97.26%), Postives = 576/583 (98.80%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANA+SSASILC S KSLRK NQTQNNR++YRQAGSRFVVRATAKEIAFDQ SR+ALQ
Sbjct: 1   MASANALSSASILCSSHKSLRKVNQTQNNRVNYRQAGSRFVVRATAKEIAFDQSSRTALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLI+ELE KARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIG+MIADAI KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGLMIADAIGKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVL+TDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLITDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSS+WEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSDWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. ExPASy TrEMBL
Match: A0A1S3CNM2 (ruBisCO large subunit-binding protein subunit alpha OS=Cucumis melo OX=3656 GN=LOC103503001 PE=3 SV=1)

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 567/583 (97.26%), Postives = 576/583 (98.80%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANA+SSASILC S KSLRK NQTQNNR++YRQAGSRFVVRATAKEIAFDQ SR+ALQ
Sbjct: 1   MASANALSSASILCSSHKSLRKVNQTQNNRVNYRQAGSRFVVRATAKEIAFDQSSRTALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLI+ELE KARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIG+MIADAI KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGLMIADAIGKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVL+TDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLITDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSS+WEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSDWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. ExPASy TrEMBL
Match: A0A5A7SW32 (RuBisCO large subunit-binding protein subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00170 PE=3 SV=1)

HSP 1 Score: 1051 bits (2717), Expect = 0.0
Identity = 565/583 (96.91%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQ 88
           MASANA+SSASILC S KSLRK NQTQNNR++YRQAGSRFVVRATAKEIAFDQ SR+ALQ
Sbjct: 1   MASANALSSASILCSSHKSLRKVNQTQNNRVNYRQAGSRFVVRATAKEIAFDQSSRTALQ 60

Query: 89  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 148
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 149 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 208
           SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLI+ELE KARAI
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 209 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 268
           EGRDDIKAVASISAGNDELIG+MIADAI KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGLMIADAIGKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 269 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 328
           YISPQFVTNPEKLIAEFENARVL+TDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLITDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 329 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 388
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTG +FQANDLGLLVENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGTQFQANDLGLLVENTTIEQLG 360

Query: 389 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 448
           LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 449 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 508
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 509 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 568
           KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSS+WEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSDWEIGYNAMTDKYENLVEAGVIDP 540

Query: 569 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 611
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 583

BLAST of Cp4.1LG16g05250 vs. TAIR 10
Match: AT2G28000.1 (chaperonin-60alpha )

HSP 1 Score: 924.5 bits (2388), Expect = 4.5e-269
Identity = 500/586 (85.32%), Postives = 546/586 (93.17%), Query Frame = 0

Query: 29  MASANAISSASILCPSQKS-LRKANQTQNNRLHY-RQAGSRFVVRATAKEIAFDQRSRSA 88
           MASANA+SSAS+LC S++S L   NQ Q  R+ Y ++   RF VRA  KEIAFDQ SR+A
Sbjct: 1   MASANALSSASVLCSSRQSKLGGGNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAA 60

Query: 89  LQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIRE 148
           LQ+GIDKLA+ VGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELP+ MENAGAALIRE
Sbjct: 61  LQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIRE 120

Query: 149 VASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKAR 208
           VASKTNDSAGDGTTTAS+LAREIIK GLL+VTSGANPVSLKRGIDKTVQGLI+EL+ KAR
Sbjct: 121 VASKTNDSAGDGTTTASILAREIIKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKAR 180

Query: 209 AIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAID 268
            ++GRDDI+AVASISAGND+LIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGM ID
Sbjct: 181 PVKGRDDIRAVASISAGNDDLIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEID 240

Query: 269 RGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTG 328
           RGYISPQFVTNPEKL+AEFENARVL+TDQKI+AIKDIIPILEKTTQLRAPLLIIAEDVTG
Sbjct: 241 RGYISPQFVTNPEKLLAEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTG 300

Query: 329 EALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQ 388
           EALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAILTGAE+ A D+ LLVEN +I+Q
Sbjct: 301 EALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQ 360

Query: 389 LGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGV 448
           LG+ARKVTISKD+TT+IADAASKDELQARIAQLKKEL ETDSVYD+EKLAERIAKLSGGV
Sbjct: 361 LGIARKVTISKDSTTLIADAASKDELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGV 420

Query: 449 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDA 508
           AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST++PAIK+  EDA
Sbjct: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDA 480

Query: 509 EEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVI 568
           +E+LGADIVQKAL++PA+LIAQNAG+EGEVVVEKI  S+WE GYNAMTD YENL EAGVI
Sbjct: 481 DERLGADIVQKALLSPAALIAQNAGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVI 540

Query: 569 DPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-TAAPQGLTI 612
           DPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 586

BLAST of Cp4.1LG16g05250 vs. TAIR 10
Match: AT5G18820.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.2e-181
Identity = 339/570 (59.47%), Postives = 445/570 (78.07%), Query Frame = 0

Query: 37  SASILCPSQKSLRKANQTQNNRLHYRQAGSRFVVRATAKEIAFDQRSRSALQSGIDKLAN 96
           S S   P+  S R++ Q    R          VVRA AK I + + SR  LQ+GIDKLA+
Sbjct: 5   SPSSFSPTTISPRRSGQRNEPR-------KFSVVRAGAKRILYGKDSREKLQAGIDKLAD 64

Query: 97  AVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVASKTNDSAG 156
           AV +TLGPRGRNVVL E  + KV+NDGVTIA++IELPD +ENAGA LI+EVA K N+SAG
Sbjct: 65  AVSITLGPRGRNVVLAEKDTIKVINDGVTIAKSIELPDTIENAGATLIQEVAIKMNESAG 124

Query: 157 DGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAIEGRDDIKA 216
           DGTTTA +LARE+IK G L +  GAN VS+K G++KTV+ L++ L+ K+  ++G++DIKA
Sbjct: 125 DGTTTAIILAREMIKAGSLAIAFGANAVSVKNGMNKTVKELVRVLQMKSIPVQGKNDIKA 184

Query: 217 VASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRGYISPQFVT 276
           VASISAGNDE +G +IA+ +EK+GPDGV+SIESSS+ ET+V VEEGM  D+GY+SP F+T
Sbjct: 185 VASISAGNDEFVGNLIAETVEKIGPDGVISIESSSTSETSVIVEEGMKFDKGYMSPHFIT 244

Query: 277 NPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEALATLVVNK 336
           N EK   EF+ A++LVTDQKI++ K+++P+LEKT+QL  PLLIIAED++ E L  LVVNK
Sbjct: 245 NQEKSTVEFDKAKILVTDQKITSAKELVPLLEKTSQLSVPLLIIAEDISAEVLEILVVNK 304

Query: 337 LRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLGLARKVTIS 396
            +G++NVA +K PG  + +KA+LQDIA++TGA++ + DLG+ +   + +QLG++R+V I+
Sbjct: 305 KQGLINVAVVKCPGMLDGKKALLQDIALMTGADYLSGDLGMSLMGATSDQLGVSRRVVIT 364

Query: 397 KDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAVIKVGAATE 456
            ++TTI+ADA++K E+QARIAQ+KK+LAETD+ Y ++K+AERIAKL+GGVAVIKVG  TE
Sbjct: 365 ANSTTIVADASTKPEIQARIAQMKKDLAETDNSYLSKKIAERIAKLTGGVAVIKVGGHTE 424

Query: 457 TELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKL-EDAEEKLGADIV 516
           TELEDRKLRIEDAKNATFAA+ EGIVPGGGA  +HL   +P IK  L ED+ E++GADIV
Sbjct: 425 TELEDRKLRIEDAKNATFAAMREGIVPGGGATYIHLLDEIPRIKKNLMEDSYEQIGADIV 484

Query: 517 QKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDPAKVTRCA 576
             AL APA  IA NAG++G VVV+K +  EW  GYNAM+ KYE+L+ AG+ DP +V+R A
Sbjct: 485 AMALTAPAMAIATNAGVDGSVVVQKTRELEWRSGYNAMSGKYEDLLNAGIADPCRVSRFA 544

Query: 577 LQNAASVAGMVLTTQAIVVEK---PKPKAP 603
           LQNA SVAG++LTTQA++VEK   PKP  P
Sbjct: 545 LQNAVSVAGIILTTQAVLVEKIKQPKPAVP 567

BLAST of Cp4.1LG16g05250 vs. TAIR 10
Match: AT5G56500.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 518.8 bits (1335), Expect = 5.7e-147
Identity = 300/587 (51.11%), Postives = 415/587 (70.70%), Query Frame = 0

Query: 31  SANAISSASILCPSQK-----SLRKANQTQNNRLHYRQAGSRFVVRATAKEIAF--DQRS 90
           SA +   +S+  PS +     S+  ++  +   +  R+A  RF     AK++ F  D  +
Sbjct: 6   SATSSMGSSLAPPSNRLSSFVSISSSSFGRTQSIAQRKA--RFPKIYAAKQLHFNKDGTA 65

Query: 91  RSALQSGIDKLANAVGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDPMENAGAA 150
              LQ+G++KLA+ VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL DP+EN GA 
Sbjct: 66  IKKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAK 125

Query: 151 LIREVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELE 210
           L+R+ ASKTND AGDGTTT+ VLA+ +I  G+  V +GANPV + RGI+KT + L+ EL+
Sbjct: 126 LVRQAASKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALVAELK 185

Query: 211 NKARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEG 270
             ++ +E   ++  VA++SAGN+  +G MIA+A+ KVG  GV+++E   S E ++ V EG
Sbjct: 186 KMSKEVED-SELADVAAVSAGNNYEVGNMIAEAMAKVGRKGVVTLEEGKSAENSLYVVEG 245

Query: 271 MAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAE 330
           M  DRGYISP FVT+ EK+ AE+EN ++ + D+KI+  +DII ILE   +   PLLIIAE
Sbjct: 246 MQFDRGYISPYFVTDSEKMCAEYENCKLFLVDKKITNARDIISILEDAIKGGYPLLIIAE 305

Query: 331 DVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENT 390
           D+  E LATLVVNKLRG + VAA+KAPGFGER+   L DIA LTGA     ++GL +E  
Sbjct: 306 DIEQEPLATLVVNKLRGTIKVAALKAPGFGERKSQYLDDIAALTGATVIREEVGLQLEKV 365

Query: 391 SIEQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKL 450
             E LG A KV ++KDTTTI+ D ++++ ++ R+ Q+K  +   +  Y+ EKL ERIAKL
Sbjct: 366 GPEVLGNAGKVVLTKDTTTIVGDGSTEEVVKKRVEQIKNLIEAAEQDYEKEKLNERIAKL 425

Query: 451 SGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDK 510
           SGGVAVI+VGA TETEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIK+ 
Sbjct: 426 SGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKET 485

Query: 511 LEDAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSE-WEIGYNAMTDKYENLV 570
           L + EEK+GADIV+KAL  P  LIA+NAG+ G VV EK+ SS+  + GYNA T KYE+L+
Sbjct: 486 LANDEEKVGADIVKKALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKHGYNAATGKYEDLM 545

Query: 571 EAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQG 609
            AG+IDP KV RC L++A+SVA   L +  +VVE  +P+  +AAP G
Sbjct: 546 AAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVEIKEPE--SAAPAG 587

BLAST of Cp4.1LG16g05250 vs. TAIR 10
Match: AT5G56500.2 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 518.8 bits (1335), Expect = 5.7e-147
Identity = 300/587 (51.11%), Postives = 415/587 (70.70%), Query Frame = 0

Query: 31  SANAISSASILCPSQK-----SLRKANQTQNNRLHYRQAGSRFVVRATAKEIAF--DQRS 90
           SA +   +S+  PS +     S+  ++  +   +  R+A  RF     AK++ F  D  +
Sbjct: 6   SATSSMGSSLAPPSNRLSSFVSISSSSFGRTQSIAQRKA--RFPKIYAAKQLHFNKDGTA 65

Query: 91  RSALQSGIDKLANAVGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDPMENAGAA 150
              LQ+G++KLA+ VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL DP+EN GA 
Sbjct: 66  IKKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAK 125

Query: 151 LIREVASKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELE 210
           L+R+ ASKTND AGDGTTT+ VLA+ +I  G+  V +GANPV + RGI+KT + L+ EL+
Sbjct: 126 LVRQAASKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIEKTTKALVAELK 185

Query: 211 NKARAIEGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEG 270
             ++ +E   ++  VA++SAGN+  +G MIA+A+ KVG  GV+++E   S E ++ V EG
Sbjct: 186 KMSKEVED-SELADVAAVSAGNNYEVGNMIAEAMAKVGRKGVVTLEEGKSAENSLYVVEG 245

Query: 271 MAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAE 330
           M  DRGYISP FVT+ EK+ AE+EN ++ + D+KI+  +DII ILE   +   PLLIIAE
Sbjct: 246 MQFDRGYISPYFVTDSEKMCAEYENCKLFLVDKKITNARDIISILEDAIKGGYPLLIIAE 305

Query: 331 DVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENT 390
           D+  E LATLVVNKLRG + VAA+KAPGFGER+   L DIA LTGA     ++GL +E  
Sbjct: 306 DIEQEPLATLVVNKLRGTIKVAALKAPGFGERKSQYLDDIAALTGATVIREEVGLQLEKV 365

Query: 391 SIEQLGLARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKL 450
             E LG A KV ++KDTTTI+ D ++++ ++ R+ Q+K  +   +  Y+ EKL ERIAKL
Sbjct: 366 GPEVLGNAGKVVLTKDTTTIVGDGSTEEVVKKRVEQIKNLIEAAEQDYEKEKLNERIAKL 425

Query: 451 SGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDK 510
           SGGVAVI+VGA TETEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIK+ 
Sbjct: 426 SGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKET 485

Query: 511 LEDAEEKLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSE-WEIGYNAMTDKYENLV 570
           L + EEK+GADIV+KAL  P  LIA+NAG+ G VV EK+ SS+  + GYNA T KYE+L+
Sbjct: 486 LANDEEKVGADIVKKALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKHGYNAATGKYEDLM 545

Query: 571 EAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQG 609
            AG+IDP KV RC L++A+SVA   L +  +VVE  +P+  +AAP G
Sbjct: 546 AAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVEIKEPE--SAAPAG 587

BLAST of Cp4.1LG16g05250 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 2.2e-146
Identity = 287/573 (50.09%), Postives = 404/573 (70.51%), Query Frame = 0

Query: 36  SSASILCPSQKSLRKANQTQNNRLHYRQ----AGSRFVVRATAKEIAF--DQRSRSALQS 95
           S  S+L P+   L  A    ++    R       SR  +   AKE+ F  D  +   LQ+
Sbjct: 10  SLGSLLAPNAIKLSSATSISSSSFGRRHNVCVRRSRPAIVCAAKELHFNKDGTTIRKLQT 69

Query: 96  GIDKLANAVGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 155
           G++KLA+ VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL DP+EN GA L+R+ A
Sbjct: 70  GVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAA 129

Query: 156 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 215
           +KTND AGDGTTT+ VLA+  I  G+  V +GANPV + RGI+KT + L+ EL+  ++ +
Sbjct: 130 AKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSKEV 189

Query: 216 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 275
           E   ++  VA++SAGN+  +G MIA+A+ KVG  GV+++E   S E  + V EGM  DRG
Sbjct: 190 ED-SELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRG 249

Query: 276 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 335
           YISP FVT+ EK+  E++N ++L+ D+K++  +D++ +LE   +   P+LIIAED+  EA
Sbjct: 250 YISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQEA 309

Query: 336 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 395
           LATLVVNKLRG L +AA+KAPGFGER+   L DIAILTGA     ++GL ++    E LG
Sbjct: 310 LATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLG 369

Query: 396 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 455
            A KV ++K+ TTI+ D  +++ +  R+ Q++  + + +  Y+ EKL ERIAKLSGGVAV
Sbjct: 370 NASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAV 429

Query: 456 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 515
           I+VGA TETEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIKD LE+ EE
Sbjct: 430 IQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDEE 489

Query: 516 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSE-WEIGYNAMTDKYENLVEAGVID 575
           K+GA+IV++AL  P  LIA+NAG+ G VV EK+ +++  + GYNA T KYE+L+ AG+ID
Sbjct: 490 KVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGIID 549

Query: 576 PAKVTRCALQNAASVAGMVLTTQAIVVEKPKPK 601
           P KV RC L++AASVA   L +  +VVE P+P+
Sbjct: 550 PTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPE 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P089261.6e-27186.39RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sati... [more]
P212386.4e-26885.32Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P212391.9e-26489.19RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS... [more]
P347941.2e-25883.93RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Brassica n... [more]
P088241.1e-24892.32RuBisCO large subunit-binding protein subunit alpha (Fragment) OS=Ricinus commun... [more]
Match NameE-valueIdentityDescription
XP_023511889.10.0100.00ruBisCO large subunit-binding protein subunit alpha [Cucurbita pepo subsp. pepo][more]
XP_022985565.10.099.31ruBisCO large subunit-binding protein subunit alpha-like isoform X1 [Cucurbita m... [more]
KAG6570801.10.099.14hypothetical protein SDJN03_29716, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022943380.10.098.97ruBisCO large subunit-binding protein subunit alpha-like [Cucurbita moschata][more]
KAG7010643.10.098.97hypothetical protein SDJN02_27438 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1JBN60.099.31ruBisCO large subunit-binding protein subunit alpha-like isoform X1 OS=Cucurbita... [more]
A0A6J1FXR20.098.97ruBisCO large subunit-binding protein subunit alpha-like OS=Cucurbita moschata O... [more]
A0A5D3DFR60.097.26RuBisCO large subunit-binding protein subunit alpha OS=Cucumis melo var. makuwa ... [more]
A0A1S3CNM20.097.26ruBisCO large subunit-binding protein subunit alpha OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7SW320.096.91RuBisCO large subunit-binding protein subunit alpha OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
AT2G28000.14.5e-26985.32chaperonin-60alpha [more]
AT5G18820.11.2e-18159.47TCP-1/cpn60 chaperonin family protein [more]
AT5G56500.15.7e-14751.11TCP-1/cpn60 chaperonin family protein [more]
AT5G56500.25.7e-14751.11TCP-1/cpn60 chaperonin family protein [more]
AT3G13470.12.2e-14650.09TCP-1/cpn60 chaperonin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 402..429
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 29..606
NoneNo IPR availablePANTHERPTHR45633:SF39BNAA04G16190D PROTEINcoord: 29..606
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 98..124
score: 40.38
coord: 338..361
score: 71.1
coord: 420..445
score: 57.23
coord: 468..489
score: 73.3
coord: 153..180
score: 58.88
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 74..598
e-value: 1.4E-222
score: 737.8
IPR001844Chaperonin Cpn60HAMAPMF_00600CH60coord: 73..609
score: 36.704365
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 75..595
e-value: 0.0
score: 822.473
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 94..597
e-value: 2.0E-95
score: 320.3
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 262..443
e-value: 2.9E-213
score: 710.9
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 254..446
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 207..480
e-value: 2.9E-213
score: 710.9
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 208..271
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 77..594
e-value: 2.9E-213
score: 710.9
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 78..593
IPR018370Chaperonin Cpn60, conserved sitePROSITEPS00296CHAPERONINS_CPN60coord: 475..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g05250.1Cp4.1LG16g05250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042026 protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity