Cp4.1LG14g03320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g03320
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Glutathione S-transferase
Location	Cp4.1LG14: 2406631 .. 2407861 (+)
RNA-Seq Expression	Cp4.1LG14g03320
Synteny	Cp4.1LG14g03320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACGAAGTGAAACTGCTGGATTTCTGGCCGAGTATGTTCGGGATGCGAGTTAGAATAGCCCTGGCCGAGAAGGGCGTAGCGTATGAATACATGGAACAAGATCTGAGGAACAAGAGCCCTTTGCTGTTGCAGATGAACCCAGTTTACAAGAAGATCCCTGTTCTTGTCCATAATGGAAGACCCATTTGTGAATCTTCCATTATCGTTCAGTACATCGATGAAGTTTGGAAGGACAAAGCGCCTCTCCTGTCGTCTGATCCTTATCAGAGGGCTCAGGCCAGATTCTGGGTGGATTTCATCGACAAGAAGGTAACTTTATTTCTTTTCGCCCTCCCCTTTTTATGATATTGTCCAAGTACAAGTTTTAGTCATCTTGCTTTTAGTTTGTCCAAAAAGCGTGATGGAGATGTATTCCTGTGTTTCCTTTTTGGTTTCTCAGAAGTCATCTCACCCAATAATGTTTCCCTCCCTCGAACAAATTACAGTGCCTTTTTTTTTTTCAGGACTATGAGCTCTCGGACCGTCTCTTTTTAGTCGAGGCTATAACTCTTTTTTCTATAGAGCCTTCGAATAAAGTCGACACTTAAGTCAAGTCACTTTTGACCTGTCTTTCTTTAGTCGAGGCTTAACTCTTTTCTCTAGAGCCTTCGAGTAAACTACCTCCTTTATTCAACATTTGAGTCATTTTTGACTATATCTTCGAAGCTATCAACTTTTTTGTTTGACATTTAAGGATTCTATTGACATGGTTTATATTAAAACTACGACTCTACAAAATAATATGATATTGTCGACTTTAAGCATAAGTTTTCATGACTTTACTTTTAATTTTTCCGAAAAGCCTCATATGAACGAAAAAACTGTAATGAAAAACTTGCAGCTTTATGATGCTGGGAGGAAGGTATGGGCCGGGAAGGGAGAAGAGGTGGAGGCAGGGAAGAAGGAGCTGATTGGAGTTCTGAAGCAGCTGGAAGAGGTTCTTGGGGAGAAGGGTTTCTTCGGAGGGGAGCGTTTGGGGTTTGTAGACATTGCTTTGATTGGATTCCACAGTTGGTTTTATAGCTATGAGGCGTTTGGTAAGTTGAGCATCGAGGCTGAGTGTCCCAAGATAATGGGTTGGGCGAAGAGATGCTTGGAGAAGGAGAGTGTTTCCAAGTCCTTGCCTGACTCCAAGAAGGTCTATGACTTTGCGGTTCAAATGAAGAAAGCACTTGGCCTTGAATGA

mRNA sequence

ATGGCGGACGAAGTGAAACTGCTGGATTTCTGGCCGAGTATGTTCGGGATGCGAGTTAGAATAGCCCTGGCCGAGAAGGGCGTAGCGTATGAATACATGGAACAAGATCTGAGGAACAAGAGCCCTTTGCTGTTGCAGATGAACCCAGTTTACAAGAAGATCCCTGTTCTTGTCCATAATGGAAGACCCATTTGTGAATCTTCCATTATCGTTCAGTACATCGATGAAGGAGAAGAGGTGGAGGCAGGGAAGAAGGAGCTGATTGGAGTTCTGAAGCAGCTGGAAGAGGTTCTTGGGGAGAAGGGTTTCTTCGGAGGGGAGCGTTTGGGGTTTGTAGACATTGCTTTGATTGGATTCCACAGTTGGTTTTATAGCTATGAGGCGTTTGGTAAGTTGAGCATCGAGGCTGAGTGTCCCAAGATAATGGGTTGGGCGAAGAGATGCTTGGAGAAGGAGAGTGTTTCCAAGTCCTTGCCTGACTCCAAGAAGGTCTATGACTTTGCGGTTCAAATGAAGAAAGCACTTGGCCTTGAATGA

Coding sequence (CDS)

ATGGCGGACGAAGTGAAACTGCTGGATTTCTGGCCGAGTATGTTCGGGATGCGAGTTAGAATAGCCCTGGCCGAGAAGGGCGTAGCGTATGAATACATGGAACAAGATCTGAGGAACAAGAGCCCTTTGCTGTTGCAGATGAACCCAGTTTACAAGAAGATCCCTGTTCTTGTCCATAATGGAAGACCCATTTGTGAATCTTCCATTATCGTTCAGTACATCGATGAAGGAGAAGAGGTGGAGGCAGGGAAGAAGGAGCTGATTGGAGTTCTGAAGCAGCTGGAAGAGGTTCTTGGGGAGAAGGGTTTCTTCGGAGGGGAGCGTTTGGGGTTTGTAGACATTGCTTTGATTGGATTCCACAGTTGGTTTTATAGCTATGAGGCGTTTGGTAAGTTGAGCATCGAGGCTGAGTGTCCCAAGATAATGGGTTGGGCGAAGAGATGCTTGGAGAAGGAGAGTGTTTCCAAGTCCTTGCCTGACTCCAAGAAGGTCTATGACTTTGCGGTTCAAATGAAGAAAGCACTTGGCCTTGAATGA

Protein sequence

MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHNGRPICESSIIVQYIDEGEEVEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECPKIMGWAKRCLEKESVSKSLPDSKKVYDFAVQMKKALGLE

Homology

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BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot
Match: Q9SHH7 (Glutathione S-transferase U25 OS=Arabidopsis thaliana OX=3702 GN=GSTU25 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.9e-63
Identity = 122/219 (55.71%), Postives = 148/219 (67.58%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEV LLDFWPSMFGMR RIAL EK V ++Y EQDL NKSP+LL+MNPV+KKIPVL+HN
Sbjct: 1   MADEVILLDFWPSMFGMRTRIALEEKNVKFDYREQDLWNKSPILLEMNPVHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G P+CES I ++YIDE                                         GEE
Sbjct: 61  GNPVCESLIQIEYIDEVWPSKTPLLPSDPYQRAQAKFWGDFIDKKVYASARLIWGAKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 179
            EAGKKE I +LK LE  LG+K +FGGE  G+VDIALIGF+SWF +YE FG  SIEAECP
Sbjct: 121 HEAGKKEFIEILKTLESELGDKTYFGGETFGYVDIALIGFYSWFEAYEKFGSFSIEAECP 180

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BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot
Match: Q03666 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 6.1e-62
Identity = 120/218 (55.05%), Postives = 145/218 (66.51%), Query Frame = 0


Query: 3   DEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHNGR 62
           +EV LLDFWPSMFGMR+RIALAEK + YEY E+DLRNKSPLLLQMNP++KKIPVL+HNG+
Sbjct: 4   EEVILLDFWPSMFGMRLRIALAEKEIKYEYKEEDLRNKSPLLLQMNPIHKKIPVLIHNGK 63

Query: 63  PICESSIIVQYIDE------------------------------------------GEEV 122
           PICES I V+YI+E                                          GEE 
Sbjct: 64  PICESIIAVEYIEEVWKDKAPNLLPSDPYDRAQARFWADYIDKKLYDFGRKLWTTKGEEQ 123

Query: 123 EAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECPK 179
           EA KK+ I  LK LE  LG+K +FGGE  GFVDIALIG++SWFY+YE FG  S EAECPK
Sbjct: 124 EAAKKDFIECLKVLEGALGDKPYFGGESFGFVDIALIGYYSWFYAYETFGNFSTEAECPK 183

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BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot
Match: P49332 (Probable glutathione S-transferase parC OS=Nicotiana tabacum OX=4097 GN=PARC PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.4e-61
Identity = 119/218 (54.59%), Postives = 145/218 (66.51%), Query Frame = 0


Query: 3   DEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHNGR 62
           +EV LLDFWPSMFGMR+RIALAEK + YEY ++DLRNKSPLLLQMNP++KKIPVL+HNG+
Sbjct: 4   EEVILLDFWPSMFGMRLRIALAEKEIKYEYKQEDLRNKSPLLLQMNPIHKKIPVLIHNGK 63

Query: 63  PICESSIIVQYIDE------------------------------------------GEEV 122
           PICES I V+YI+E                                          GEE 
Sbjct: 64  PICESIIAVEYIEEVWKDKAPSLLPSDPYDRAQARFWADYIDKKLYDFGRKLWATKGEEQ 123

Query: 123 EAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECPK 179
           EA KK+ I  LK LE  LG++ +FGGE  GFVDIALIGF+SWFY+YE FG  S EAECPK
Sbjct: 124 EAAKKDFIECLKVLEGALGDRPYFGGESFGFVDIALIGFYSWFYAYETFGNFSTEAECPK 183

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot
Match: P46417 (Glutathione S-transferase 3 OS=Glycine max OX=3847 GN=GST3 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.5e-60
Identity = 116/216 (53.70%), Postives = 143/216 (66.20%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           M+DEV LLD W SM+GMR RIALAEKGV YEY E++L N+SPLLLQMNP++KKIPVL+HN
Sbjct: 1   MSDEVVLLDTWASMYGMRARIALAEKGVRYEYKEENLMNRSPLLLQMNPIHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G+PICES+IIVQYIDE                                         GEE
Sbjct: 61  GKPICESAIIVQYIDEVWNDKSPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 176
            E GKKELI + KQLEE L +K F+G +  GFVD+ LI F SWFY+YE +G   +E ECP
Sbjct: 121 HEEGKKELISIFKQLEETLTDKPFYGDDTFGFVDLCLITFSSWFYTYETYGNFKMEEECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot
Match: Q9SHH6 (Glutathione S-transferase U24 OS=Arabidopsis thaliana OX=3702 GN=GSTU24 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 4.1e-58
Identity = 120/219 (54.79%), Postives = 145/219 (66.21%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEV LLDFW SMFGMR RIALAEK V Y++ E+DL NKS LLL+MNPV+KKIPVL+HN
Sbjct: 1   MADEVILLDFWASMFGMRTRIALAEKRVKYDHREEDLWNKSSLLLEMNPVHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G+P+CES I ++YIDE                                         GEE
Sbjct: 61  GKPVCESLIQIEYIDETWPDNNPLLPSDPYKRAHAKFWADFIDKKVNVTARRIWAVKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 179
            EA  KELI +LK LE  LG+K +FG E  G+VDIALIGFHSWF  YE FG +SIE+EC 
Sbjct: 121 QEAA-KELIEILKTLESELGDKKYFGDETFGYVDIALIGFHSWFAVYEKFGNVSIESECS 180

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BLAST of Cp4.1LG14g03320 vs. NCBI nr
Match: XP_023552156.1 (probable glutathione S-transferase parC [Cucurbita pepo subsp. pepo] >XP_023552157.1 probable glutathione S-transferase parC [Cucurbita pepo subsp. pepo] >XP_023552158.1 probable glutathione S-transferase parC [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337 bits (865), Expect = 7.30e-116
Identity = 178/219 (81.28%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLSSDPYQRAQARFWVDFIDKKLYDAGRKVWAGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. NCBI nr
Match: KAG6577064.1 (hypothetical protein SDJN03_24638, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 334 bits (856), Expect = 1.72e-114
Identity = 176/219 (80.37%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYQRAQARFWVDFIDKKLYDAGRKVWTGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. NCBI nr
Match: XP_022931331.1 (probable glutathione S-transferase [Cucurbita moschata])

HSP 1 Score: 334 bits (856), Expect = 1.72e-114
Identity = 176/219 (80.37%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYHRAQARFWVDFIDKKLYDAGRKVWTGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. NCBI nr
Match: XP_022984351.1 (probable glutathione S-transferase parC [Cucurbita maxima])

HSP 1 Score: 328 bits (840), Expect = 4.70e-112
Identity = 173/219 (79.00%), Postives = 175/219 (79.91%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYQRAQARFWVDFIDKKLYDAGRKVWAGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAG KELIGVLKQLEEVLGEK FFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGMKELIGVLKQLEEVLGEKSFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. NCBI nr
Match: BAC21261.1 (glutathione S-transferase [Cucurbita maxima])

HSP 1 Score: 321 bits (823), Expect = 1.77e-109
Identity = 172/219 (78.54%), Postives = 175/219 (79.91%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGV  EYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVRIEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYQRAQARFWVDFIDKKLYDAGRKVWTGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGK ELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFY+YEAFGKLSIEAECP
Sbjct: 121 VEAGK-ELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYTYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL
Match: A0A6J1ETD8 (probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111437553 PE=3 SV=1)

HSP 1 Score: 334 bits (856), Expect = 8.31e-115
Identity = 176/219 (80.37%), Postives = 178/219 (81.28%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYHRAQARFWVDFIDKKLYDAGRKVWTGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL
Match: A0A6J1J502 (probable glutathione S-transferase parC OS=Cucurbita maxima OX=3661 GN=LOC111482680 PE=3 SV=1)

HSP 1 Score: 328 bits (840), Expect = 2.28e-112
Identity = 173/219 (79.00%), Postives = 175/219 (79.91%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYQRAQARFWVDFIDKKLYDAGRKVWAGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAG KELIGVLKQLEEVLGEK FFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP
Sbjct: 121 VEAGMKELIGVLKQLEEVLGEKSFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL
Match: Q8H9E7 (Glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=Puga PE=2 SV=1)

HSP 1 Score: 321 bits (823), Expect = 8.56e-110
Identity = 172/219 (78.54%), Postives = 175/219 (79.91%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEVKLLDFWPSMFG+RVRIALAEKGV  EYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MADEVKLLDFWPSMFGIRVRIALAEKGVRIEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           GRPICESSIIVQYIDE                                         GEE
Sbjct: 61  GRPICESSIIVQYIDEVWKDKAPLLPSDPYQRAQARFWVDFIDKKLYDAGRKVWTGKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
           VEAGK ELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFY+YEAFGKLSIEAECP
Sbjct: 121 VEAGK-ELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYTYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL
Match: A0A6J1JA96 (probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111482681 PE=3 SV=1)

HSP 1 Score: 315 bits (808), Expect = 1.71e-107
Identity = 167/219 (76.26%), Postives = 172/219 (78.54%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MA+EVKLLDFWPSMFG+RVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPV+KKIPVLVHN
Sbjct: 1   MAEEVKLLDFWPSMFGIRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G PICESSII+QYIDE                                         GEE
Sbjct: 61  GTPICESSIILQYIDEVWNDKAPLLPSDPYHRAQARFWVDFIDKKLCDAGRKVWGGRGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 178
            EAG KELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGF+SWFYSYEAFGKLSIEAECP
Sbjct: 121 AEAGMKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFYSWFYSYEAFGKLSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL
Match: A0A1S4DYW1 (probable glutathione S-transferase parC isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492981 PE=4 SV=1)

HSP 1 Score: 285 bits (729), Expect = 1.44e-95
Identity = 140/211 (66.35%), Postives = 165/211 (78.20%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MA+EVKLLDFWPSMFGMRVRIALA+KGVAYEY+E+DLRNKSPLLLQMNP++KKIPVL+HN
Sbjct: 1   MAEEVKLLDFWPSMFGMRVRIALAQKGVAYEYIEEDLRNKSPLLLQMNPIHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE----------------------------------GEEVEAGKKE 120
           G+PICESSIIVQYIDE                                  GEE EAGKKE
Sbjct: 61  GKPICESSIIVQYIDEVWNDKAPLLPSHPYDRAQARFWVDFIDKKIWATKGEEHEAGKKE 120

Query: 121 LIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECPKIMGWAK 177
           +I +LKQLE+VLGEK +FGGE +G +DIALIGF+SWFY+YE  GK SIEAECPKI+ W K
Sbjct: 121 MIEILKQLEQVLGEKDYFGGESMGIIDIALIGFYSWFYTYETIGKFSIEAECPKIISWGK 180

[Close]

BLAST of Cp4.1LG14g03320 vs. TAIR 10
Match: AT1G17180.1 (glutathione S-transferase TAU 25 )

HSP 1 Score: 243.4 bits (620), Expect = 1.4e-64
Identity = 122/219 (55.71%), Postives = 148/219 (67.58%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEV LLDFWPSMFGMR RIAL EK V ++Y EQDL NKSP+LL+MNPV+KKIPVL+HN
Sbjct: 1   MADEVILLDFWPSMFGMRTRIALEEKNVKFDYREQDLWNKSPILLEMNPVHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G P+CES I ++YIDE                                         GEE
Sbjct: 61  GNPVCESLIQIEYIDEVWPSKTPLLPSDPYQRAQAKFWGDFIDKKVYASARLIWGAKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 179
            EAGKKE I +LK LE  LG+K +FGGE  G+VDIALIGF+SWF +YE FG  SIEAECP
Sbjct: 121 HEAGKKEFIEILKTLESELGDKTYFGGETFGYVDIALIGFYSWFEAYEKFGSFSIEAECP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. TAIR 10
Match: AT1G17170.1 (glutathione S-transferase TAU 24 )

HSP 1 Score: 225.7 bits (574), Expect = 2.9e-59
Identity = 120/219 (54.79%), Postives = 145/219 (66.21%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MADEV LLDFW SMFGMR RIALAEK V Y++ E+DL NKS LLL+MNPV+KKIPVL+HN
Sbjct: 1   MADEVILLDFWASMFGMRTRIALAEKRVKYDHREEDLWNKSSLLLEMNPVHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G+P+CES I ++YIDE                                         GEE
Sbjct: 61  GKPVCESLIQIEYIDETWPDNNPLLPSDPYKRAHAKFWADFIDKKVNVTARRIWAVKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 179
            EA  KELI +LK LE  LG+K +FG E  G+VDIALIGFHSWF  YE FG +SIE+EC 
Sbjct: 121 QEAA-KELIEILKTLESELGDKKYFGDETFGYVDIALIGFHSWFAVYEKFGNVSIESECS 180

[Close]

BLAST of Cp4.1LG14g03320 vs. TAIR 10
Match: AT1G78380.1 (glutathione S-transferase TAU 19 )

HSP 1 Score: 224.6 bits (571), Expect = 6.5e-59
Identity = 114/214 (53.27%), Postives = 143/214 (66.82%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MA+EV LLDFWPSMFGMR RIAL EKGV +EY E+DLRNKSPLLLQMNP++KKIPVL+HN
Sbjct: 1   MANEVILLDFWPSMFGMRTRIALREKGVEFEYREEDLRNKSPLLLQMNPIHKKIPVLIHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G+P+ ES I VQYIDE                                         GEE
Sbjct: 61  GKPVNESIIQVQYIDEVWSHKNPILPSDPYLRAQARFWADFIDKKLYDAQRKVWATKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 174
            EAGKK+ I +LK LE  LG+K +F G+  G+VDIALIGF++WF +YE F   SIE+E P
Sbjct: 121 QEAGKKDFIEILKTLESELGDKPYFSGDDFGYVDIALIGFYTWFPAYEKFANFSIESEVP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. TAIR 10
Match: AT1G78370.1 (glutathione S-transferase TAU 20 )

HSP 1 Score: 221.1 bits (562), Expect = 7.2e-58
Identity = 112/214 (52.34%), Postives = 139/214 (64.95%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQDLRNKSPLLLQMNPVYKKIPVLVHN 60
           MA+   LLD+WPSMFGMR R+AL EKGV +EY E+D  NKSPLLLQ NP++KKIPVLVHN
Sbjct: 1   MANLPILLDYWPSMFGMRARVALREKGVEFEYREEDFSNKSPLLLQSNPIHKKIPVLVHN 60

Query: 61  GRPICESSIIVQYIDE-----------------------------------------GEE 120
           G+P+CES  +VQY+DE                                         GEE
Sbjct: 61  GKPVCESLNVVQYVDEAWPEKNPFFPSDPYGRAQARFWADFVDKKFTDAQFKVWGKKGEE 120

Query: 121 VEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEAECP 174
            EAGKKE I  +K LE  LG+K +FGG+  G+VDI+LI F SWF +YE FG  SIE+E P
Sbjct: 121 QEAGKKEFIEAVKILESELGDKPYFGGDSFGYVDISLITFSSWFQAYEKFGNFSIESESP 180

[Close]

BLAST of Cp4.1LG14g03320 vs. TAIR 10
Match: AT1G78360.1 (glutathione S-transferase TAU 21 )

HSP 1 Score: 217.2 bits (552), Expect = 1.0e-56
Identity = 112/222 (50.45%), Postives = 142/222 (63.96%), Query Frame = 0


Query: 1   MADEVKLLDFWPSMFGMRVRIALAEKGVAYEYMEQD-LRNKSPLLLQMNPVYKKIPVLVH 60
           MA EV LL FWPSMFGMR  IAL EKGV YEY E+D + NKSPLLL+MNP++K IPVL+H
Sbjct: 1   MAAEVILLGFWPSMFGMRTMIALEEKGVKYEYREEDVINNKSPLLLEMNPIHKTIPVLIH 60

Query: 61  NGRPICESSIIVQYIDE------------------------------------------- 120
           NG+P+ ES I +QYIDE                                           
Sbjct: 61  NGKPVLESLIQIQYIDEVWSDNNSFLPSDPYHRAQALFWADFIDKKEQLYVCGRKTWATK 120

Query: 121 GEEVEAGKKELIGVLKQLEEVLGEKGFFGGERLGFVDIALIGFHSWFYSYEAFGKLSIEA 179
           GEE+EA  KE I +LK L+  LGEK +FGG++ GFVDI LIGF+SWF +Y+ FG  SIE 
Sbjct: 121 GEELEAANKEFIEILKTLQCELGEKPYFGGDKFGFVDIVLIGFYSWFPAYQKFGNFSIEP 180

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g03320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHH7	1.9e-63	55.71	Glutathione S-transferase U25 OS=Arabidopsis thaliana OX=3702 GN=GSTU25 PE=1 SV=...	[more]
Q03666	6.1e-62	55.05	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49332	1.4e-61	54.59	Probable glutathione S-transferase parC OS=Nicotiana tabacum OX=4097 GN=PARC PE=...	[more]
P46417	1.5e-60	53.70	Glutathione S-transferase 3 OS=Glycine max OX=3847 GN=GST3 PE=1 SV=1	[more]
Q9SHH6	4.1e-58	54.79	Glutathione S-transferase U24 OS=Arabidopsis thaliana OX=3702 GN=GSTU24 PE=2 SV=...	[more]

BLAST of Cp4.1LG14g03320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023552156.1	7.30e-116	81.28	probable glutathione S-transferase parC [Cucurbita pepo subsp. pepo] >XP_0235521...	[more]
KAG6577064.1	1.72e-114	80.37	hypothetical protein SDJN03_24638, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022931331.1	1.72e-114	80.37	probable glutathione S-transferase [Cucurbita moschata]	[more]
XP_022984351.1	4.70e-112	79.00	probable glutathione S-transferase parC [Cucurbita maxima]	[more]
BAC21261.1	1.77e-109	78.54	glutathione S-transferase [Cucurbita maxima]	[more]

BLAST of Cp4.1LG14g03320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETD8	8.31e-115	80.37	probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111437553...	[more]
A0A6J1J502	2.28e-112	79.00	probable glutathione S-transferase parC OS=Cucurbita maxima OX=3661 GN=LOC111482...	[more]
Q8H9E7	8.56e-110	78.54	Glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=Puga PE=2 SV=1	[more]
A0A6J1JA96	1.71e-107	76.26	probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111482681 P...	[more]
A0A1S4DYW1	1.44e-95	66.35	probable glutathione S-transferase parC isoform X2 OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of Cp4.1LG14g03320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17180.1	1.4e-64	55.71	glutathione S-transferase TAU 25	[more]
AT1G17170.1	2.9e-59	54.79	glutathione S-transferase TAU 24	[more]
AT1G78380.1	6.5e-59	53.27	glutathione S-transferase TAU 19	[more]
AT1G78370.1	7.2e-58	52.34	glutathione S-transferase TAU 20	[more]
AT1G78360.1	1.0e-56	50.45	glutathione S-transferase TAU 21	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 76..151 e-value: 3.6E-10 score: 39.9
None	No IPR available	GENE3D	1.20.1050.10		coord: 79..151 e-value: 2.6E-14 score: 55.1
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..78 e-value: 8.9E-39 score: 133.4
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 4..76
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 76..173
None	No IPR available	PANTHER	PTHR11260:SF662	S-TRANSFERASE TAU 22, PUTATIVE-RELATED	coord: 4..76
None	No IPR available	PANTHER	PTHR11260:SF662	S-TRANSFERASE TAU 22, PUTATIVE-RELATED	coord: 76..173
None	No IPR available	CDD	cd03058	GST_N_Tau	coord: 5..76 e-value: 1.61713E-45 score: 142.417
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 5..76 e-value: 1.5E-19 score: 70.1
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 3..82 score: 20.519226
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 22..175 score: 17.052881
IPR045074	Glutathione S-transferases Tau, C-terminal alpha helical domain, plant	CDD	cd03185	GST_C_Tau	coord: 76..171 e-value: 1.25404E-32 score: 111.502
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 73..173
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 4..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g03320.1	Cp4.1LG14g03320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0005515	protein binding