Cp4.1LG13g11850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g11850
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Tubulin-folding cofactor E
Location	Cp4.1LG13: 9270984 .. 9275387 (-)
RNA-Seq Expression	Cp4.1LG13g11850
Synteny	Cp4.1LG13g11850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACAAACAACTCTACTCTTTGATCCAATATTTAGAATAACTAATATTTCGAATGATCCAATTCTACTCATCTCCACGCGGAGGAGAAAAGATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGCCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCCCAGAATCTGAGTTTCGGCATTTCGCTTCTCCAAGCGTTGGAACTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGGTATGTATCTGCGCTATCAATATCTGTCTAGTTGCTCCTAACATGGCTGCCTAATTGTGGTATATCGTACACTTAATCTTACTGCCTAAACTCAAATTTTATTTTCAACTTGTTTAATGTAATTCAACGGTTGCGCTGAATTTGTTGGTAATGGGTTGTCCTGCGTTGCCATTTGCTCTCTTGCAGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTACATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAGTAAGTTTCTTGACCTCATTGAAGTATACATTTGTCTTCTTAACTTTATATACGTTGTTGATTTTCAAGATGCGGTTTATATGAGAAACATTGTATTAAACCAGCGTAGCAAAAGACCATACTCAGACATTTGGAGCTGAAGCATCTGATCATTGATGATCACCCTTTTAAAGTTGATGCCAAGCTTCTTTTGATGAATTTTACGAGATCTTTCTTTGGAATGTTTATGTAAAGAAACAATAAAAAATTTGGGAGAGAAAAGATATAGGATCACTGTTTACGGGATAATCTTTTGTCCTGTCGCTATTTATAATTCTAATGTGATTATATATGTTTCATCTGTAATGTCTCCATTTCAGACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGTACGTGTTGAAGAAAACCATTATTGTTCACCCTCTTAGCAACCCACACTGAAAATGTACAGTCATAACTTGCAATTAACTGTGGTTTTAAACACCAGTCTTCGACTCCACCATAGAGTTTTTGTTGTTTTTTCAGAAAATACTTGGGTTTATGTTACGTTGATCAGTTGTTTACCTCTTCAGGGTGAAAACTTGGTAGAACACATTGTGTTCCTTTTTTCGCTTGAAAGATGAGGGTTGTCCTTTGCTTTCTAATATGTGGTTGTATAAATTCAATCAGTTCGACGTATATATGCCTTTTTTCAATGGCCAAACCAGTGAATTCTTAACGTAAATGTCTTTGAGGTTTGTTCTGTATCGTGCCCTGTGGGAAGGTCTGATACCCTGCTGAACAATTTTTTCGATCTAGTCCCATCAAAGAAATAAAAAAGATGGAAAGAAAGAAAGAACGAGCATCTTTCTTTTGTTTTCCAAATTGTAATTTCTTTGGTTGTTGGCAGTTTAGTAGTTCTGAACATTATTGTCTTATTGTCAACTTCATATATTCATGCAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTAGTTGTTAGTATGGTTGAGATATATATATATTTTTTTCTTCTCCTTATCCTTTATATATTCATATTATATTTGGAGTTAATTTTATATGATAGGTTGAAACACTTAAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGGTACTGATTGAGATCCTTAAACTTTGTAATTGTTGGATGAATACATAATAATTTAGTCTCTTGTTGCAGTTGCATTGCATTGATTATTGATTTCATCTGATGGGAAATAATTTAGTCTTTCAATGCTTGTGAGTTGCAGAAATGAATCAAATAATAGAAGGGTTTATAAAGTCGATTAATCCGTTTACAATTGTGTCACTTAGTTCTATAATACGAACAACTAACCTGTATTTCTGTTGAGATCTCGTTTTCAGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGTTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGGTATTGTTGTTTTATTATTATTTTGTTCTTCTATTGGGACAATTGGCTGTTGTCGAACCCTCGCTGATTTCACTGTATTTCCTTGGAATCTTTGACTATGCTATCAGCTTGGAGCAGATTCAGTTGAATAACAACAAGTTGAGTCGTATATTTTATCCTAGCTTCGATGAATTACACAAGTTATTTGGAGATGTTGAATCACAAGGAGACTGTTCTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGTACATTATCAAGGTTCTTTAACAATTGTGATCTCTTTATAAATTCTTTTAATTACTTCTCTGACTTGTTATATTTTTGTATGTACTTCAGGAGGCAACAACATTGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGTAAGATTTTAATATATATTATTTCAATTCGAGGGATAGTTCTTGAATTGAAAAAGCTAATAACATTGGCAGATACATTATCGACTCGAACTTCAACTTCTTTATCGTTCACTTCTCTCCATCGTTTTATTTATTCATTTCTTTTTCCTTCAAGGACATCAGGCTATCAGAGAATCCTATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTTGTTTGGAAGCATTTGCAGTCCTTTTCTTTTCTCATTATTACATTACCGTTAACCCGAAAACTTTTTTCTCATATATTTTTTAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGGTGCGGAAATCAATATTTTATATGCATTATTAGTTTCCTCCCCTTCTTTGTACTAACCAGGATATATTGGTAATATGAGTAGTCTTGATATAACCAGAGGGAGTAAGGGTGTGAGATGGTAGGCTACATTTTGGGGGAGTCTATAGTCTATGTTTAAGATGTGAAGTTATTATGAAGAACATTTGAATCCTTTCAGGAAATTGTGGTTCATTCGAAGTTATAAATTTCGAACATTTGTATCCGTTTATTCAGCGATTTCAATTCTTCTTGCAGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTAACTGCATTCAACTAAAACTGAACTTACCCCGTGCTGCGCCCCATGAACGTTATTTTTGTTGATATATTTATGCATCAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGACTCATATGTAGGAATGAATCTTGAATTTGAGATATTTGACATTTTAGAACATTTCCTTATCGTCGCTGAATAGTTGTTTGATAAATTTATCCAGCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTACTGTCCTTGCTTTTTTATTTTAACATGCTGTTTCACTGCTTCACTCTAAATGACTCTTTCCATCTTTAGGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTGAAGTTGTATCTTCAAGAACAGGTTTGGTCTCCTAATTACTTAATACCCAACTCATTAACCTTCTGCAACTGAACACACCTTACATTCTCAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGAAGGAAATAAACCCCCCATAGGTTCTTCAATTTTTTTCCTTCTTTGGTTTTAACTCTCTGGCATTCCCATGATTCTTGCTCCCCAGATTTGGAAGATGTTTTTAATTGTTGGTTTTCTATCGGATGTTCTTATATCATTTGACCAAGATTCACAGCTTAAACAAATCGTGAAATCACACTGAATTGCCTGATCTTCTATTCTATTTATCTGCCTCATATTT

mRNA sequence

AAAAAACAAACAACTCTACTCTTTGATCCAATATTTAGAATAACTAATATTTCGAATGATCCAATTCTACTCATCTCCACGCGGAGGAGAAAAGATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGCCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCCCAGAATCTGAGTTTCGGCATTTCGCTTCTCCAAGCGTTGGAACTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTACATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTGAAACACTTAAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGTTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGCTTGGAGCAGATTCAGTTGAATAACAACAAGTTGAGTCGTATATTTTATCCTAGCTTCGATGAATTACACAAGTTATTTGGAGATGTTGAATCACAAGGAGACTGTTCTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGAGGCAACAACATTGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGACATCAGGCTATCAGAGAATCCTATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGACTCATATCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTGAAGTTGTATCTTCAAGAACAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGAAGGAAATAAACCCCCCATAGGTTCTTCAATTTTTTTCCTTCTTTGGTTTTAACTCTCTGGCATTCCCATGATTCTTGCTCCCCAGATTTGGAAGATGTTTTTAATTGTTGGTTTTCTATCGGATGTTCTTATATCATTTGACCAAGATTCACAGCTTAAACAAATCGTGAAATCACACTGAATTGCCTGATCTTCTATTCTATTTATCTGCCTCATATTT

Coding sequence (CDS)

ATGATCCAATTCTACTCATCTCCACGCGGAGGAGAAAAGATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGCCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCCCAGAATCTGAGTTTCGGCATTTCGCTTCTCCAAGCGTTGGAACTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTACATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTGAAACACTTAAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGTTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGCTTGGAGCAGATTCAGTTGAATAACAACAAGTTGAGTCGTATATTTTATCCTAGCTTCGATGAATTACACAAGTTATTTGGAGATGTTGAATCACAAGGAGACTGTTCTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGAGGCAACAACATTGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGACATCAGGCTATCAGAGAATCCTATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGACTCATATCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTGAAGTTGTATCTTCAAGAACAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGA

Protein sequence

MIQFYSSPRGGEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES

Homology

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BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot
Match: Q8GRL7 (Tubulin-folding cofactor E OS=Arabidopsis thaliana OX=3702 GN=TFCE PE=2 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 7.2e-186
Identity = 337/530 (63.58%), Postives = 412/530 (77.74%), Query Frame = 0


Query: 24  FRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQAKSE 83
           F +GQR H + D RRVGTV +VG +EGYSGTWIGVDWD D +GKH+GS+NGV YF  +S+
Sbjct: 10  FIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQ 69

Query: 84  RSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDK 143
            S SFVR+QNLS GI+LLQALELRYR  STK+EEDEMYVLSA ++RVS+Q +G D I+DK
Sbjct: 70  SSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDK 129

Query: 144 LSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALV 203
           LSRFEELTS SLSY+GVSS G    +GS+LPN+K LDLTGNL+SDW++I  +C+QL AL 
Sbjct: 130 LSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALT 189

Query: 204 ALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKISTV 263
            L LS N LS DI    QL +IR+LVLNN+G++W QVE L+ SLP IEELHLMGN IST+
Sbjct: 190 TLNLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGNMISTI 249

Query: 264 TPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFDE 323
           T  SSS   Q FN LRLLNL++NCI +WSE+LKL QL  LEQ+ LN NKLSRIF      
Sbjct: 250 TSTSSSD-DQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLSRIF------ 309

Query: 324 LHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMR 383
              + G   S+    PF +L  L LG NNI DLAS+D LN FPQL+DIRLSENPI+DP+R
Sbjct: 310 -QSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLVDIRLSENPISDPVR 369

Query: 384 GGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEEL 443
           GG+PR+VL+ARL+KVQ++NGSEV  RE++DSEIRYVRMVMSKL+    E   LHPRF EL
Sbjct: 370 GGVPRFVLVARLTKVQVLNGSEVRAREKKDSEIRYVRMVMSKLNDKSGEIELLHPRFYEL 429

Query: 444 KRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLC 503
           K+ +GIED R S    GP+ +ASGLISITL CVG S+GEKP LTKKLP + TVGKLK+L 
Sbjct: 430 KKLHGIEDERASAENSGPKNIASGLISITLKCVGPSMGEKPHLTKKLPGSITVGKLKILS 489

Query: 504 ESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 553
           E+FFKLK ++ +L+LQE+ SP P  L+D+ A+L+D+G+ +GS +LVDEES
Sbjct: 490 ENFFKLKSIKPRLFLQEEGSPFPTALDDETATLLDVGICDGSTLLVDEES 531

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot
Match: Q5U378 (Tubulin-specific chaperone E OS=Danio rerio OX=7955 GN=tbce PE=2 SV=2)

HSP 1 Score: 222.6 bits (566), Expect = 1.1e-56
Identity = 177/548 (32.30%), Postives = 275/548 (50.18%), Query Frame = 0


Query: 20  LQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQ 79
           + L+  +G+R    G+    GTV +VG +   +G W+GV+WD    GKHDGS +GVRYF 
Sbjct: 1   MMLDEAVGRRVCCDGER---GTVRYVGPVPPTAGVWLGVEWDHPERGKHDGSHDGVRYFT 60

Query: 80  AKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDL 139
            +    GSFVR Q  SFG+  + AL+ RY  +  +   +EM + S      +V  VG + 
Sbjct: 61  CRHPTGGSFVRPQKASFGVDYVTALKQRYEVEIEEVTAEEMKISSK-----TVVMVGFEN 120

Query: 140 IKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQL 199
           +K K S  + LT V L    VS+PG   +I +  P V+ LDL+GNLLS W+ ++ I +QL
Sbjct: 121 VKKKQS-VKNLTEVGLRRCEVSAPGPENEIRNTTPFVQSLDLSGNLLSSWEVLAAITEQL 180

Query: 200 QALVALILSNNLLSRDISGPVQL----NHIRILVLNNTGITWMQVETLKHSLPTIEELHL 259
            +L  L LS+N LS   S P  L    +H+R+L +N+  +TW QV         +EEL+L
Sbjct: 181 DSLQELHLSHNRLSIS-SAPSSLSSAFSHLRVLSINSCALTWTQVLHCAPMWQQVEELYL 240

Query: 260 MGNKIS-TVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 319
             N I+  + PE           L +L+L NN I +   +L++  L  LE++ L++  LS
Sbjct: 241 ADNNITELLRPE------HVLQALTVLDLSNNQIAQ-ETVLEISHLPRLERLNLSSTSLS 300

Query: 320 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 379
            I           F DV +    + F  L+ L L  NNI +   ++ L   P L+ +   
Sbjct: 301 EI----------KFSDVPAGKKTTLFPALKELLLDDNNISEWRVVNELEKLPSLVYLSCR 360

Query: 380 ENPIADPMRG-GIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKL------- 439
            NP+    +     R ++IARL ++++++  ++   ERR +E+ Y +M  S         
Sbjct: 361 RNPLLHKEKNLETARQIMIARLGQLELLDMRQILSDERRGAELDYCKMFGSAWLRAGGHR 420

Query: 440 ---DSNPE-ETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGE 499
               +NP  + +  HPR+  L + YG  D    +    P  L + L++IT  C      E
Sbjct: 421 EAEKNNPNTDFMTEHPRYLTLIQKYGAPDEG-ELREQKPFALKNQLLTITFLC--PEDLE 480

Query: 500 KPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKL-YLQEQDSPVPIPLEDDMASLMDLGV 549
           + P+ KKLP +  V K+K L     KL  + LKL Y   + +   I +++D+  L    V
Sbjct: 481 RKPIEKKLPGSMIVQKVKGLLHRLLKLPGVELKLTYTCAKMADREIEIDNDLKPLQFYSV 518

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot
Match: Q5U508 (Tubulin-specific chaperone E OS=Xenopus laevis OX=8355 GN=tbce PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.4e-56
Identity = 161/525 (30.67%), Postives = 259/525 (49.33%), Query Frame = 0


Query: 41  TVAFVGTLEGYSGTWIGVDWDDS-NGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGISL 100
           TV +VG +    G W+GV+WD+   GKH+G+  G +YF       GSF+R +  +FG+  
Sbjct: 22  TVRYVGNVPPTPGLWLGVEWDNHLRGKHNGTHEGTKYFTCSHPTGGSFIRLKKANFGVDF 81

Query: 101 LQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGV 160
           L AL  RY     K E++E  V+     + +V+ VG + I+++ S+  +L  VSL    V
Sbjct: 82  LAALRKRY---GLKSEQNEELVIG----KKTVELVGFESIQEEQSKLNKLKDVSLRECAV 141

Query: 161 SSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVALILSNNLL---SRDIS 220
           S+ G   QI    PN+   DL+ NL S W+ ++ I  QL+ L +L LS N L   S   S
Sbjct: 142 SNAGEKGQICHSCPNIMTADLSKNLFSSWESLAHISSQLENLTSLDLSENKLNPSSNPSS 201

Query: 221 GPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKISTVTPESSSPMVQGFNL 280
                 ++++L LN TG+ W ++       P +EELHL+ N IS +        V     
Sbjct: 202 LATSFCNLKVLSLNRTGMKWNEILQCASMWPALEELHLVSNDISLL-----EQPVNNLQN 261

Query: 281 LRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFDELHKLFGDVESQGDC 340
           L +L++ NN IV+ +++  +  L  L+Q+ ++NN +S I +P  D            G  
Sbjct: 262 LTILDISNNKIVDGNQLHTIAFLPRLKQVIVSNNIISSISFPDVD-----------FGHT 321

Query: 341 SPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIAD-PMRGGIPRYVLIARLS 400
           + F +L +L + GNNI +   I+ L+    L  +    NP+ D        R ++IA++ 
Sbjct: 322 AMFISLTSLAVNGNNISEWCVINELHKLLHLESLNCHGNPLMDLDKNPETVRQLIIAKIE 381

Query: 401 KVQIINGSEVTPRERRDSEIRYVRMV-----------MSKLDSNPEETLRLHPRFEELKR 460
            ++ +N +E+ P ERR +E+ Y +M              + +    + L+ HPR+  L +
Sbjct: 382 NLKFLNKTEIFPTERRGAELDYRKMFGNEWLKAGGSQNEEFNKPSRDFLQDHPRYSALIK 441

Query: 461 FYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLCES 520
            YG  D    +    P  L + L+++T+ C      +K P+ KKLP + TV K+K L   
Sbjct: 442 KYGAPDEG-ELKQQQPFALKNQLLTLTIQC--PEKPDKKPIQKKLPDSMTVQKVKGLLYR 501

Query: 521 FFKLKCMRLKL-YLQEQDSPVPIPLEDDMASLMDLGVANGSNILV 549
             K+    LKL Y   +     I LE+D+  L    V NG  +LV
Sbjct: 502 LLKVPGSDLKLSYQSSKMEGKEIELENDLKPLQFYSVENGDCLLV 520

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot
Match: Q5FVQ9 (Tubulin-specific chaperone E OS=Rattus norvegicus OX=10116 GN=Tbce PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.8e-54
Identity = 164/539 (30.43%), Postives = 261/539 (48.42%), Query Frame = 0


Query: 26  LGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDD-SNGKHDGSINGVRYFQAKSERS 85
           +G+R    G+     TV F G +   +G W+GV+WD+   GKHDGS  G  YF+ +    
Sbjct: 10  IGRRVEVNGE---YATVRFCGAVPPVAGLWLGVEWDNPERGKHDGSHEGTMYFKCRHPTG 69

Query: 86  GSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLS 145
           GSFVR   ++FG   L AL+ RY      +++++   L    K+  VQ +G + I  K S
Sbjct: 70  GSFVRPNIVNFGEDFLTALKKRYVLTDGPDDDEKSCSLKVGSKQ--VQTIGFEHITKKQS 129

Query: 146 RFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVAL 205
           +   L  +SL    VS  G   +I    PN++ +DL+ NLLS W ++ +I +QL+ L AL
Sbjct: 130 QLRSLQDISLWKCAVSCAGERGRIAEACPNIRVVDLSKNLLSTWDEVILIAEQLKDLEAL 189

Query: 206 ILSNNLLSRDISGPV---QLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKIST 265
            LS N L      P      + ++ LVLN TGITW +V     S P ++EL+L  N IS 
Sbjct: 190 DLSENKLQFPSDSPTLTRTFSTLKTLVLNKTGITWTEVLHCAPSWPVLQELYLKSNGISI 249

Query: 266 VTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFD 325
                S   V     LRLL+L +N  ++ S++  +  L  LE + L++  LS I +P   
Sbjct: 250 -----SERPVNALQNLRLLDLSSNPSIDESQLCLIAYLPRLEHLLLSDIGLSSIHFP--- 309

Query: 326 ELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPM 385
                  D E     S F  L  L +  N I + + I+ L+    L  +  + NP+    
Sbjct: 310 -------DAEIGCKTSMFPALTYLIVNDNQISEWSFINELDKLQSLQALSCARNPLT--- 369

Query: 386 RGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMV---MSKLDSNPE-------- 445
           +G     ++IA++ +++ +N  ++ P ERR +E+ Y +       K   +P+        
Sbjct: 370 KGDKAEEIIIAKIGQLKTLNRCQILPEERRGAELDYRKAFGKEWRKAGGHPDPDRNRPSA 429

Query: 446 ETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLP 505
           E L  HPR++ L   YG  ++   +    P  L + L+++ + C  ++  E+  L K+LP
Sbjct: 430 EFLSAHPRYQLLCCKYGAPEDE-ELKTQQPFMLKNQLLTLKIKC--SNQPEQQILEKQLP 489

Query: 506 LTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSP-VPIPLEDDMASLMDLGVANGSNILV 549
            + T+ K+K L     K+    L L  +    P   I LE+D+  L    V NG  +LV
Sbjct: 490 DSMTIQKVKGLLSRLLKVPVSELLLSYESSKMPGREIELENDLQPLQFYSVENGDCLLV 522

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot
Match: Q8CIV8 (Tubulin-specific chaperone E OS=Mus musculus OX=10090 GN=Tbce PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.1e-53
Identity = 162/539 (30.06%), Postives = 261/539 (48.42%), Query Frame = 0


Query: 26  LGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDD-SNGKHDGSINGVRYFQAKSERS 85
           +G+R    G+     TV F G +   +G W+GV+WD+   GKHDGS  G  YF+ +    
Sbjct: 10  IGRRVEVNGE---YATVRFCGAVPPVAGLWLGVEWDNPERGKHDGSHEGTMYFKCRHPTG 69

Query: 86  GSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLS 145
           GSFVR   ++FG   L AL+ RY  +   ++++    L    K+  VQ +G + I  K S
Sbjct: 70  GSFVRPSKVNFGDDFLTALKKRYVLEDGPDDDENSCSLKVGSKQ--VQTIGFEHITKKQS 129

Query: 146 RFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVAL 205
           +   L  +SL    VS  G   +I    PN++ ++L+ NLLS W ++ +I +QL+ L AL
Sbjct: 130 QLRALQDISLWNCAVSHAGEQGRIAEACPNIRVVNLSKNLLSTWDEVVLIAEQLKDLEAL 189

Query: 206 ILSNNLLSRDISGPV---QLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKIST 265
            LS N L      P      + ++ LVLN TGITW +V     S P +EEL+L  N IS 
Sbjct: 190 DLSENKLQFPSDSPTLTRTFSTLKTLVLNKTGITWTEVLHCAPSWPVLEELYLKSNNISI 249

Query: 266 VTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFD 325
                S   V     +RLL+L +N  ++ S++  +  L  LE + L++  LS I +P   
Sbjct: 250 -----SERPVNVLQKMRLLDLSSNPSIDESQLSLIADLPRLEHLVLSDIGLSSIHFP--- 309

Query: 326 ELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPM 385
                  D E     S F  L+ L +  N I + + I+ L+    L  +  + NP++   
Sbjct: 310 -------DAEIGCKTSMFPALKYLIVNDNQISEWSFINELDKLQSLQALSCTRNPLS--- 369

Query: 386 RGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMV---MSKLDSNPEET------ 445
           +      ++IA++++++ +N  ++ P ERR +E+ Y +       K   +P+        
Sbjct: 370 KADKAEEIIIAKIAQLRTLNRCQILPEERRGAELDYRKAFGNEWRKAGGHPDPDKNRPNA 429

Query: 446 --LRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLP 505
             L  HPR++ L   YG  ++   +    P  L   L+++ + C  ++  E+  L K+LP
Sbjct: 430 AFLSAHPRYQLLCCKYGAPEDE-ELKTQQPFMLKKQLLTLKIKC--SNQPERQILEKQLP 489

Query: 506 LTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSP-VPIPLEDDMASLMDLGVANGSNILV 549
            + TV K+K L     K+    L L  +    P   I LE+D+  L    V NG  +LV
Sbjct: 490 DSMTVQKVKGLLSRLLKVPVSELLLSYESSKMPGREIELENDLQPLQFYSVENGDCLLV 522

[Close]

BLAST of Cp4.1LG13g11850 vs. NCBI nr
Match: XP_023551442.1 (tubulin-folding cofactor E [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1084 bits (2803), Expect = 0.0
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0


Query: 1   MIQFYSSPRGGEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDW 60
           MIQFYSSPRGGEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDW
Sbjct: 1   MIQFYSSPRGGEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDW 60

Query: 61  DDSNGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMY 120
           DDSNGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMY
Sbjct: 61  DDSNGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMY 120

Query: 121 VLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDL 180
           VLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDL
Sbjct: 121 VLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDL 180

Query: 181 TGNLLSDWKDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVE 240
           TGNLLSDWKDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVE
Sbjct: 181 TGNLLSDWKDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVE 240

Query: 241 TLKHSLPTIEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLR 300
           TLKHSLPTIEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLR
Sbjct: 241 TLKHSLPTIEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLR 300

Query: 301 SLEQIQLNNNKLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDF 360
           SLEQIQLNNNKLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDF
Sbjct: 301 SLEQIQLNNNKLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDF 360

Query: 361 LNSFPQLIDIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRM 420
           LNSFPQLIDIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRM
Sbjct: 361 LNSFPQLIDIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRM 420

Query: 421 VMSKLDSNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIG 480
           VMSKLDSNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIG
Sbjct: 421 VMSKLDSNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIG 480

Query: 481 EKPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGV 540
           EKPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGV
Sbjct: 481 EKPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGV 540

Query: 541 ANGSNILVDEES 552
           ANGSNILVDEES
Sbjct: 541 ANGSNILVDEES 552

[Close]

BLAST of Cp4.1LG13g11850 vs. NCBI nr
Match: KAG6578311.1 (Tubulin-folding cofactor E, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1052 bits (2720), Expect = 0.0
Identity = 536/539 (99.44%), Postives = 537/539 (99.63%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFRL QR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLDQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNK+STVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS
Sbjct: 241 LMGNKMSTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

[Close]

BLAST of Cp4.1LG13g11850 vs. NCBI nr
Match: XP_022939765.1 (tubulin-folding cofactor E [Cucurbita moschata])

HSP 1 Score: 1044 bits (2700), Expect = 0.0
Identity = 531/539 (98.52%), Postives = 534/539 (99.07%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFRLGQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCI EWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPS DELHKLFGDVE QGDC PFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

[Close]

BLAST of Cp4.1LG13g11850 vs. NCBI nr
Match: KAG7015887.1 (Tubulin-folding cofactor E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1039 bits (2686), Expect = 0.0
Identity = 532/546 (97.44%), Postives = 534/546 (97.80%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFRLGQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCI EWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPS DELHKLFGDVE QGDC PFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIR-------YVRMVMSKLD 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIR       YVRMVMSKLD
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRDFNSSCSYVRMVMSKLD 420

Query: 434 SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT 493
           SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT
Sbjct: 421 SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT 480

Query: 494 KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI 552
           KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI
Sbjct: 481 KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI 540

[Close]

BLAST of Cp4.1LG13g11850 vs. NCBI nr
Match: XP_022993434.1 (tubulin-folding cofactor E [Cucurbita maxima])

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 528/539 (97.96%), Postives = 532/539 (98.70%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFR GQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRPGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGN LSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNFLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQL+HIRILVLNNTGITWMQVETLKHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLSHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNKISTVTPESSSP+VQGFNLLRLLNLENNCI EWSEILKLGQLRSLEQIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPIVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPS DELHKLFGDVESQGDC PFRNL+NLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVESQGDCLPFRNLQNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           TVGKLKMLCESFFKLKC+RLKLYLQEQDSPVPI LEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCVRLKLYLQEQDSPVPIQLEDDMASLMDLGVANGSNILVDEES 539

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL
Match: A0A6J1FNN5 (Tubulin-folding cofactor E OS=Cucurbita moschata OX=3662 GN=LOC111445549 PE=3 SV=1)

HSP 1 Score: 1044 bits (2700), Expect = 0.0
Identity = 531/539 (98.52%), Postives = 534/539 (99.07%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFRLGQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCI EWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPS DELHKLFGDVE QGDC PFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL
Match: A0A6J1K072 (Tubulin-folding cofactor E OS=Cucurbita maxima OX=3661 GN=LOC111489449 PE=3 SV=1)

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 528/539 (97.96%), Postives = 532/539 (98.70%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQGSIQLQLEFR GQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRPGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGN LSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNFLSDWKDISI 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           ICDQLQALVALILSNNLLSRDISGPVQL+HIRILVLNNTGITWMQVETLKHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLSHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGNKISTVTPESSSP+VQGFNLLRLLNLENNCI EWSEILKLGQLRSLEQIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPIVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
           RIFYPS DELHKLFGDVESQGDC PFRNL+NLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVESQGDCLPFRNLQNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           TVGKLKMLCESFFKLKC+RLKLYLQEQDSPVPI LEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCVRLKLYLQEQDSPVPIQLEDDMASLMDLGVANGSNILVDEES 539

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL
Match: A0A1S3BEN9 (Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1)

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 476/544 (87.50%), Postives = 501/544 (92.10%), Query Frame = 0


Query: 9   RGGEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHD 68
           R GEKMQ S Q Q EFRLGQR HFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHD
Sbjct: 5   RNGEKMQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHD 64

Query: 69  GSINGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKR 128
           GSINGVRYFQAKSERSGSFVR QNLS GISLLQAL+LRYRGDSTKEEEDEMYVLSASDKR
Sbjct: 65  GSINGVRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKR 124

Query: 129 VSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDW 188
           VSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDW
Sbjct: 125 VSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDW 184

Query: 189 KDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPT 248
           KDIS +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE LKHSLP 
Sbjct: 185 KDISTLCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPA 244

Query: 249 IEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLN 308
           IEELHLMGN IS V PESSS MV+GFNLLRLLNLENNCI EW+EILKLGQLRSLEQIQLN
Sbjct: 245 IEELHLMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLN 304

Query: 309 NNKLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLI 368
           NNKLS IFYP+ DELH+LFGDVE QGDC PF+NLR LFLGGNNIDDLASID LNSFP LI
Sbjct: 305 NNKLSHIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLI 364

Query: 369 DIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSN 428
           DIRLSENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N
Sbjct: 365 DIRLSENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGN 424

Query: 429 PEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKK 488
            E+T RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKK
Sbjct: 425 REKTFRLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKK 484

Query: 489 LPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILV 548
           LP  T+VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILV
Sbjct: 485 LPPATSVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILV 544

Query: 549 DEES 552
           DEES
Sbjct: 545 DEES 547

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL
Match: A0A1S3BDZ7 (Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 475/542 (87.64%), Postives = 500/542 (92.25%), Query Frame = 0


Query: 11  GEKMQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGS 70
           GEKMQ S Q Q EFRLGQR HFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHDGS
Sbjct: 39  GEKMQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHDGS 98

Query: 71  INGVRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVS 130
           INGVRYFQAKSERSGSFVR QNLS GISLLQAL+LRYRGDSTKEEEDEMYVLSASDKRVS
Sbjct: 99  INGVRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKRVS 158

Query: 131 VQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKD 190
           VQFVGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDWKD
Sbjct: 159 VQFVGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDWKD 218

Query: 191 ISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIE 250
           IS +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE LKHSLP IE
Sbjct: 219 ISTLCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPAIE 278

Query: 251 ELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNN 310
           ELHLMGN IS V PESSS MV+GFNLLRLLNLENNCI EW+EILKLGQLRSLEQIQLNNN
Sbjct: 279 ELHLMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLNNN 338

Query: 311 KLSRIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDI 370
           KLS IFYP+ DELH+LFGDVE QGDC PF+NLR LFLGGNNIDDLASID LNSFP LIDI
Sbjct: 339 KLSHIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLIDI 398

Query: 371 RLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPE 430
           RLSENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N E
Sbjct: 399 RLSENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGNRE 458

Query: 431 ETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLP 490
           +T RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKKLP
Sbjct: 459 KTFRLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKKLP 518

Query: 491 LTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDE 550
             T+VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILVDE
Sbjct: 519 PATSVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILVDE 578

Query: 551 ES 552
           ES
Sbjct: 579 ES 579

[Close]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL
Match: A0A1S3BEU4 (Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 472/539 (87.57%), Postives = 497/539 (92.21%), Query Frame = 0


Query: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73
           MQ S Q Q EFRLGQR HFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHDGSING
Sbjct: 1   MQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHDGSING 60

Query: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133
           VRYFQAKSERSGSFVR QNLS GISLLQAL+LRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193
           VGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDWKDIS 
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDWKDIST 180

Query: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253
           +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE LKHSLP IEELH
Sbjct: 181 LCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPAIEELH 240

Query: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313
           LMGN IS V PESSS MV+GFNLLRLLNLENNCI EW+EILKLGQLRSLEQIQLNNNKLS
Sbjct: 241 LMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLNNNKLS 300

Query: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373
            IFYP+ DELH+LFGDVE QGDC PF+NLR LFLGGNNIDDLASID LNSFP LIDIRLS
Sbjct: 301 HIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLIDIRLS 360

Query: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433
           ENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N E+T 
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGNREKTF 420

Query: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493
           RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKKLP  T
Sbjct: 421 RLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKKLPPAT 480

Query: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552
           +VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILVDEES
Sbjct: 481 SVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILVDEES 538

[Close]

BLAST of Cp4.1LG13g11850 vs. TAIR 10
Match: AT1G71440.1 (tubulin folding cofactor E / Pfifferling (PFI) )

HSP 1 Score: 651.7 bits (1680), Expect = 5.1e-187
Identity = 337/530 (63.58%), Postives = 412/530 (77.74%), Query Frame = 0


Query: 24  FRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQAKSE 83
           F +GQR H + D RRVGTV +VG +EGYSGTWIGVDWD D +GKH+GS+NGV YF  +S+
Sbjct: 10  FIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQ 69

Query: 84  RSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDK 143
            S SFVR+QNLS GI+LLQALELRYR  STK+EEDEMYVLSA ++RVS+Q +G D I+DK
Sbjct: 70  SSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDK 129

Query: 144 LSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALV 203
           LSRFEELTS SLSY+GVSS G    +GS+LPN+K LDLTGNL+SDW++I  +C+QL AL 
Sbjct: 130 LSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALT 189

Query: 204 ALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELHLMGNKISTV 263
            L LS N LS DI    QL +IR+LVLNN+G++W QVE L+ SLP IEELHLMGN IST+
Sbjct: 190 TLNLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGNMISTI 249

Query: 264 TPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLSRIFYPSFDE 323
           T  SSS   Q FN LRLLNL++NCI +WSE+LKL QL  LEQ+ LN NKLSRIF      
Sbjct: 250 TSTSSSD-DQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLSRIF------ 309

Query: 324 LHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMR 383
              + G   S+    PF +L  L LG NNI DLAS+D LN FPQL+DIRLSENPI+DP+R
Sbjct: 310 -QSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLVDIRLSENPISDPVR 369

Query: 384 GGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEEL 443
           GG+PR+VL+ARL+KVQ++NGSEV  RE++DSEIRYVRMVMSKL+    E   LHPRF EL
Sbjct: 370 GGVPRFVLVARLTKVQVLNGSEVRAREKKDSEIRYVRMVMSKLNDKSGEIELLHPRFYEL 429

Query: 444 KRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLC 503
           K+ +GIED R S    GP+ +ASGLISITL CVG S+GEKP LTKKLP + TVGKLK+L 
Sbjct: 430 KKLHGIEDERASAENSGPKNIASGLISITLKCVGPSMGEKPHLTKKLPGSITVGKLKILS 489

Query: 504 ESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 553
           E+FFKLK ++ +L+LQE+ SP P  L+D+ A+L+D+G+ +GS +LVDEES
Sbjct: 490 ENFFKLKSIKPRLFLQEEGSPFPTALDDETATLLDVGICDGSTLLVDEES 531

[Close]

BLAST of Cp4.1LG13g11850 vs. TAIR 10
Match: AT3G10220.1 (tubulin folding cofactor B )

HSP 1 Score: 44.7 bits (104), Expect = 2.9e-04
Identity = 24/73 (32.88%), Postives = 36/73 (49.32%), Query Frame = 0


Query: 25  RLGQRAHFVGDPRRVGTVAFVGTLEGYS-GTWIGVDWDDSNGKHDGSINGVRYFQAKSER 84
           ++G R       +R G V +VG  E    G W+G+ +D+  GKHDG + G R+F+     
Sbjct: 160 KVGDRCQVEPGEKR-GMVKYVGRAESLGPGYWVGIQYDEPLGKHDGMVKGTRFFEC-PRL 219

Query: 85  SGSFVRAQNLSFG 97
            G  VR   +  G
Sbjct: 220 QGGMVRPDKVKVG 230

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g11850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GRL7	7.2e-186	63.58	Tubulin-folding cofactor E OS=Arabidopsis thaliana OX=3702 GN=TFCE PE=2 SV=1	[more]
Q5U378	1.1e-56	32.30	Tubulin-specific chaperone E OS=Danio rerio OX=7955 GN=tbce PE=2 SV=2	[more]
Q5U508	1.4e-56	30.67	Tubulin-specific chaperone E OS=Xenopus laevis OX=8355 GN=tbce PE=2 SV=1	[more]
Q5FVQ9	3.8e-54	30.43	Tubulin-specific chaperone E OS=Rattus norvegicus OX=10116 GN=Tbce PE=2 SV=1	[more]
Q8CIV8	1.1e-53	30.06	Tubulin-specific chaperone E OS=Mus musculus OX=10090 GN=Tbce PE=1 SV=1	[more]

BLAST of Cp4.1LG13g11850 vs. NCBI nr


Match Name	Identity	Description
XP_023551442.1	100.00	tubulin-folding cofactor E [Cucurbita pepo subsp. pepo]	[more]
KAG6578311.1	99.44	Tubulin-folding cofactor E, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022939765.1	98.52	tubulin-folding cofactor E [Cucurbita moschata]	[more]
KAG7015887.1	97.44	Tubulin-folding cofactor E [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022993434.1	97.96	tubulin-folding cofactor E [Cucurbita maxima]	[more]

BLAST of Cp4.1LG13g11850 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1FNN5	98.52	Tubulin-folding cofactor E OS=Cucurbita moschata OX=3662 GN=LOC111445549 PE=3 SV...	[more]
A0A6J1K072	97.96	Tubulin-folding cofactor E OS=Cucurbita maxima OX=3661 GN=LOC111489449 PE=3 SV=1	[more]
A0A1S3BEN9	87.50	Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1	[more]
A0A1S3BDZ7	87.64	Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1	[more]
A0A1S3BEU4	87.57	Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1	[more]

BLAST of Cp4.1LG13g11850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71440.1	5.1e-187	63.58	tubulin folding cofactor E / Pfifferling (PFI)	[more]
AT3G10220.1	2.9e-04	32.88	tubulin folding cofactor B	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003591	Leucine-rich repeat, typical subtype	SMART	SM00369	LRR_typ_2	coord: 221..244 e-value: 400.0 score: 0.2 coord: 172..195 e-value: 180.0 score: 3.0 coord: 246..269 e-value: 51.0 score: 7.5 coord: 299..318 e-value: 71.0 score: 6.3 coord: 339..362 e-value: 38.0 score: 8.5
IPR000938	CAP Gly-rich domain	SMART	SM01052	CAP_GLY_2	coord: 26..94 e-value: 1.1E-26 score: 104.6
IPR000938	CAP Gly-rich domain	PFAM	PF01302	CAP_GLY	coord: 27..92 e-value: 1.4E-19 score: 69.8
IPR000938	CAP Gly-rich domain	PROSITE	PS00845	CAP_GLY_1	coord: 46..77
IPR000938	CAP Gly-rich domain	PROSITE	PS50245	CAP_GLY_2	coord: 46..89 score: 16.923649
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 218..326 e-value: 1.3E-11 score: 45.8
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 327..425 e-value: 2.3E-12 score: 48.2
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 101..217 e-value: 7.7E-10 score: 40.2
IPR036859	CAP Gly-rich domain superfamily	GENE3D	2.30.30.190		coord: 16..99 e-value: 5.9E-21 score: 75.9
IPR036859	CAP Gly-rich domain superfamily	SUPERFAMILY	74924	Cap-Gly domain	coord: 19..160
None	No IPR available	GENE3D	3.10.20.90		coord: 459..552 e-value: 3.9E-18 score: 67.2
None	No IPR available	PANTHER	PTHR15140:SF6	TUBULIN-SPECIFIC CHAPERONE E	coord: 24..550
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 140..401
IPR044994	Tubulin-specific chaperone E	PANTHER	PTHR15140	TUBULIN-SPECIFIC CHAPERONE E	coord: 24..550
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 341..362 score: 8.674542
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 276..297 score: 7.095899
IPR044079	TBCE, ubiquitin-like (Ubl) domain	CDD	cd17044	Ubl_TBCE	coord: 467..549 e-value: 1.22437E-22 score: 89.9467
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 463..549

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g11850.1	Cp4.1LG13g11850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000226	microtubule cytoskeleton organization
biological_process	GO:0007023	post-chaperonin tubulin folding pathway
biological_process	GO:0007021	tubulin complex assembly
biological_process	GO:0006457	protein folding
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0043014	alpha-tubulin binding
molecular_function	GO:0005515	protein binding