Cp4.1LG13g08880 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g08880
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmetalloendoproteinase 5-MMP-like
LocationCp4.1LG13: 7829773 .. 7832292 (+)
RNA-Seq ExpressionCp4.1LG13g08880
SyntenyCp4.1LG13g08880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGACGAACCCAATTCTCATACGGACGCAATCGACGAGGCCTTGGAGTCATCCATTAAATCATATGAGAAATTCTTCAAGCTGAACGTGAGCGGGGTTCTGGACAAGGAGACGCTAGATCAAACGTGGAAGCCGCGCTGTGGGGTAACAAGTCATGGCCGGTTGAGAAGAGGCACCTGACGTACGGATTCATCCACAATTACCCACCAGAGCAGGTGGGTCGAGTGATGCGAGGCTTTGAGACATGGGAAGCCAACACCCATTTTACATTCTCCCGGTCAGCGAGGGTCCAAACGGCTGACATACTGTCTAGCTTGGAGAAAGAGGCGTGGGGAAGGGGTGTTCCTTTTTTACGGTGTGACCTGGAGTCGGTGGCGTTGCATGAGATAGGACATGCTCTTGGGCTTGCCCGCTCTAACATTTGCCGTCATGTGGCCTCACATGGACTTTGGAGTCCAAAAGACCCACTTGCATGATGATGATCTTAATGCTATTCGTGCTGTATACGGCCCATAAAATTATAAGTCCCGATGGACCAACAAGACTCTCACAATGGTATGATATTGTCCACTTTGACCATAAGACTTCGTATCAATAAATATAATATTATTTGATTATAAACCTATGATCATTTTCTAAATTAACAAAAAAAAATTCTTTTGTAATTTCTTACACAGGAATTCAGAAAATACCTGATCAAATGACATTTTCTTTTGATCCAATTTTTTTTTCTTCTCCTTATCATTCAGGAAAGACAAAAAAAATTCAGGGTAGCAAACATTCTCGATAATTTCTCTTAGATTCGAATCCATAGCTGATGATAGAACTATAATAAATATTTTCTGTTTCATACTCACATGAACCCATATCCTTGCTTTTCGATCCATCTCTAATTCTAATCTTCCTCCCCAATCAAATATTATGGTGCCAGTATAAACCGAAATTTCGTTATGGTCCAATTCTGACTGATAATAGATACCGAAGCTTTGCAATATTTGACTGATCACAATTTTGTATAGTCCATTTACTATAGAAGTTCTAAGAAATTCATTTTCCAATAAAAATTGTTTGTTCTTGCATATCCCTACCCGTTTTCCAAATTAATCCTGCAGATATATATGTGGGACTTTCATCCATCAACCGCCCTTTACATCTATTTCTCAGCCACAAATAAAAAATGGAAATGGAAGTGATTATGATATTAATAGTATATATATACATATACATATATTTTATTTAATTTTAAGTCTTTAAATTAATATTTAATATATAAAATTCAAAAAAAATAAAAATAAAATATTTATTTGATACAAATTTTAATCAATACATTGAATAAAATATATTATTTCCATTTTGGGAAGATTATTTTACACATTTAGTACGTAAAAAATTGCTATTATGGGGACTTTCTTTTTCATTTTCCTCTTTTTTCTTTCCTAATATTGTCTTTTAAATCACATGTAAATTAAATTAATTATGTCCTAAAATCTTTCCAATTAAATTAAATTAATTAATTCTCCGAATAATAATTTACTTTTATTAAAATTGATTTTTTAAGATTCGAAAATATTTGTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAATAGAGTTTCCAAATAGCACACCTCTGGCCTTAGCTTTAAAACGGCGGCCCCAAACTCCAAAACTATATGCACAAATGGGTTTCAAATTATGGGTCCAACTCTTCCTATTACTTGCCTTCATCGCGCCCCACTTGGCCTCCGCCCGTAATCTTCAGGGTAGCCGCAAGGGCGACAACAGGAAAGGCATCCATGGCGTCAAGGCTTACCTTCAACGCTACGGCTACTACGTGACCCAAAACGAAACCAATTCTCAAACAAATGCATTCGACGAGGCCTTGGAGGAATCAATTAAATCATACCAGAAATTCTTCAAGCTTAACGTGAGCGGGGCTTTGGACAAGGAGACGCTGGATCAAATGGCGAAGCCTCGATGTGGGGTGTCGGATAATTTCACGACGGCGGGTGGGAAGCTGGGTGGTTCTTATTATGCTTTCTTTCCAGGACGCCGCATTTGGGCGCCTGAAAAGAGGCATTTAACGTACGGATTTATCCACGATTACCCATCCAAGTATGTGCCTCAAGTGATGCGGGCTTTTGCCACATGGGAAGCCAACACCCATTTTACATTCTCCCAAGCTGTGGGAGGCCAAACGGCTGACATAGTTTTAAGCTTCGAGAGAAGGGATCATGGAGATGGGCATCCGTTCGATGGACTTGGGGGAGCATTGGCCCATTGTTTTGGGCCTATTGATGGGAGAGTCCACTTTGACGCCCAGGAGATTTGGGATTGGGGTATTCGTATATTACAGAATGACTTGGAGTCCGTGGCATTGCATGAGATTGGGCATGCCCTTGGGCTTGGCCATACCGACGTTCAAGATGCCGTCATGTGGCCTACCATGAACTTTGGGTCCAGGAAGACTCGCTTGCATGATGATGATATTAATGGTATTCATGCTCTATATGGAAAT

mRNA sequence

AAAGACGAACCCAATTCTCATACGGACGCAATCGACGAGGCCTTGGAGTCATCCATTAAATCATATGAGAAATTCTTCAAGCTGAACCGGGGTTCTGGACAAGGAGACGCTAGATCAAACGTGGAAGCCGCGCTGTGGGGTAACAAGTCATGGCCGGTTGAGAAGAGGCACCTGACGTACGGATTCATCCACAATTACCCACCAGAGCAGGTGGGTCGAGTGATGCGAGGCTTTGAGACATGGGAAGCCAACACCCATTTTACATTCTCCCGGTCAGCGAGGGTCCAAACGGCTGACATACTGTCTAGCTTGGAGAAAGAGGCGTGGGGAAGGGGTGTTCCTTTTTTACGGTGTGACCTGGAGTCGGTGGCGTTGCATGAGATAGGACATGCTCTTGGGCTTGCCCGCTCTAACATTTGCCGTCATGGTAGCCGCAAGGGCGACAACAGGAAAGGCATCCATGGCGTCAAGGCTTACCTTCAACGCTACGGCTACTACGTGACCCAAAACGAAACCAATTCTCAAACAAATGCATTCGACGAGGCCTTGGAGGAATCAATTAAATCATACCAGAAATTCTTCAAGCTTAACGTGAGCGGGGCTTTGGACAAGGAGACGCTGGATCAAATGGCGAAGCCTCGATGTGGGGTGTCGGATAATTTCACGACGGCGGGTGGGAAGCTGGGTGGTTCTTATTATGCTTTCTTTCCAGGACGCCGCATTTGGGCGCCTGAAAAGAGGCATTTAACGTACGGATTTATCCACGATTACCCATCCAAGTATGTGCCTCAAGTGATGCGGGCTTTTGCCACATGGGAAGCCAACACCCATTTTACATTCTCCCAAGCTGTGGGAGGCCAAACGGCTGACATAGTTTTAAGCTTCGAGAGAAGGGATCATGGAGATGGGCATCCGTTCGATGGACTTGGGGGAGCATTGGCCCATTGTTTTGGGCCTATTGATGGGAGAGTCCACTTTGACGCCCAGGAGATTTGGGATTGGGGTATTCGTATATTACAGAATGACTTGGAGTCCGTGGCATTGCATGAGATTGGGCATGCCCTTGGGCTTGGCCATACCGACGTTCAAGATGCCGTCATGTGGCCTACCATGAACTTTGGGTCCAGGAAGACTCGCTTGCATGATGATGATATTAATGGTATTCATGCTCTATATGGAAAT

Coding sequence (CDS)

AAAGACGAACCCAATTCTCATACGGACGCAATCGACGAGGCCTTGGAGTCATCCATTAAATCATATGAGAAATTCTTCAAGCTGAACCGGGGTTCTGGACAAGGAGACGCTAGATCAAACGTGGAAGCCGCGCTGTGGGGTAACAAGTCATGGCCGGTTGAGAAGAGGCACCTGACGTACGGATTCATCCACAATTACCCACCAGAGCAGGTGGGTCGAGTGATGCGAGGCTTTGAGACATGGGAAGCCAACACCCATTTTACATTCTCCCGGTCAGCGAGGGTCCAAACGGCTGACATACTGTCTAGCTTGGAGAAAGAGGCGTGGGGAAGGGGTGTTCCTTTTTTACGGTGTGACCTGGAGTCGGTGGCGTTGCATGAGATAGGACATGCTCTTGGGCTTGCCCGCTCTAACATTTGCCGTCATGGTAGCCGCAAGGGCGACAACAGGAAAGGCATCCATGGCGTCAAGGCTTACCTTCAACGCTACGGCTACTACGTGACCCAAAACGAAACCAATTCTCAAACAAATGCATTCGACGAGGCCTTGGAGGAATCAATTAAATCATACCAGAAATTCTTCAAGCTTAACGTGAGCGGGGCTTTGGACAAGGAGACGCTGGATCAAATGGCGAAGCCTCGATGTGGGGTGTCGGATAATTTCACGACGGCGGGTGGGAAGCTGGGTGGTTCTTATTATGCTTTCTTTCCAGGACGCCGCATTTGGGCGCCTGAAAAGAGGCATTTAACGTACGGATTTATCCACGATTACCCATCCAAGTATGTGCCTCAAGTGATGCGGGCTTTTGCCACATGGGAAGCCAACACCCATTTTACATTCTCCCAAGCTGTGGGAGGCCAAACGGCTGACATAGTTTTAAGCTTCGAGAGAAGGGATCATGGAGATGGGCATCCGTTCGATGGACTTGGGGGAGCATTGGCCCATTGTTTTGGGCCTATTGATGGGAGAGTCCACTTTGACGCCCAGGAGATTTGGGATTGGGGTATTCGTATATTACAGAATGACTTGGAGTCCGTGGCATTGCATGAGATTGGGCATGCCCTTGGGCTTGGCCATACCGACGTTCAAGATGCCGTCATGTGGCCTACCATGAACTTTGGGTCCAGGAAGACTCGCTTGCATGATGATGATATTAATGGTATTCATGCTCTATATGGAAAT

Protein sequence

KDEPNSHTDAIDEALESSIKSYEKFFKLNRGSGQGDARSNVEAALWGNKSWPVEKRHLTYGFIHNYPPEQVGRVMRGFETWEANTHFTFSRSARVQTADILSSLEKEAWGRGVPFLRCDLESVALHEIGHALGLARSNICRHGSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGALDKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDGRVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHDDDINGIHALYGN
Homology
BLAST of Cp4.1LG13g08880 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.0e-50
Identity = 113/268 (42.16%), Postives = 145/268 (54.10%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G+N  G+  +K Y +R+GY  T   T + T+ FD+ L+ +I +YQK F L V+G L
Sbjct: 50  GCHIGENINGLSKLKQYFRRFGYITT---TGNCTDDFDDVLQSAINTYQKNFNLKVTGKL 109

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGGSY------YAFFPGRRIWAPEKRHLTYGFI-- 262
           D  TL Q+ KPRCG  D         GG        Y+FFPG+  W   KR LTY F   
Sbjct: 110 DSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQ 169

Query: 263 HDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALA 322
           ++   +      RAF  W   T   F+++     ADIV+ F   +HGDG PFDG  G LA
Sbjct: 170 NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLA 229

Query: 323 HCFGPIDGRVHFDAQEIW-----DWGIRILQ----NDLESVALHEIGHALGLGHTDVQDA 382
           H   P  G +H D  E W     +   RIL      DLESVA+HEIGH LGLGH+ V+DA
Sbjct: 230 HASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDA 289

Query: 383 VMWPTMNFGSRKTRLHDDDINGIHALYG 394
           +M+P ++ G RK  L  DDI GI  LYG
Sbjct: 290 IMFPAISGGDRKVELAKDDIEGIQHLYG 314

BLAST of Cp4.1LG13g08880 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.1e-46
Identity = 107/277 (38.63%), Postives = 145/277 (52.35%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G N  G++ +K Y QR+G Y+ +  + + T+ FD+ L+ +++ YQ  F LNV+G L
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFG-YIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGEL 108

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGG-----------------SYYAFFPGRRIWAPE 262
           D  T+  +  PRCG  D         GG                   Y  FPG   W   
Sbjct: 109 DALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRN 168

Query: 263 KRHLTYGFIHDYP-SKYVPQVM-RAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDG 322
           +R LTY F    P ++ V  V  RAF  W   T   F+ +    T+DI + F   DHGDG
Sbjct: 169 RRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDG 228

Query: 323 HPFDGLGGALAHCFGPIDGRVHFDAQEIW------DWGIRILQN-DLESVALHEIGHALG 382
            PFDG+ G LAH F P  G+ H DA E W      D  + +    DLESVA+HEIGH LG
Sbjct: 229 EPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLG 288

Query: 383 LGHTDVQDAVMWPTMNFGSRKTRLHDDDINGIHALYG 394
           LGH+ V++++M+PT+  G RK  L +DD+ GI  LYG
Sbjct: 289 LGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

BLAST of Cp4.1LG13g08880 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 4.7e-46
Identity = 107/260 (41.15%), Postives = 140/260 (53.85%), Query Frame = 0

Query: 147 GDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGALDKET 206
           G N KG+  VK Y    GY    N  +   N FD+ L  +IK+YQK + LNV+G  D  T
Sbjct: 48  GQNYKGLSNVKNYFHHLGY--IPNAPHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 207 LDQMAKPRCGVSD-----NFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHD--YPS 266
           L Q+  PRCGV D     N TT+ G +  S Y FF     W      LTY F  +     
Sbjct: 108 LKQIMTPRCGVPDIIINTNKTTSFGMI--SDYTFFKDMPRWQAGTTQLTYAFSPEPRLDD 167

Query: 267 KYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGP 326
            +   + RAF+ W    +  F +    +TA+I + F  ++HGD +PFDG GG L H F P
Sbjct: 168 TFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAP 227

Query: 327 IDGRVHFDAQEIWDWGIRILQN------DLESVALHEIGHALGLGHTDVQDAVMWPTMNF 386
            DGR HFDA E W     + ++      DLESVA+HEIGH LGLGH+    A+M+P++  
Sbjct: 228 TDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPP 287

Query: 387 GSRKTRLHDDDINGIHALYG 394
            +RK  L  DDI+GI  LYG
Sbjct: 288 RTRKVNLAQDDIDGIRKLYG 302

BLAST of Cp4.1LG13g08880 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.1e-46
Identity = 106/281 (37.72%), Postives = 147/281 (52.31%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G    G++ +K Y Q +GY    N + + T+ FD+ L+ +++ YQ+ F+LNV+G L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 203 DKETLDQMAKPRCGVSD-----------------NFTTAGGKLGG-SYYAFFPGRRIWAP 262
           D+ TL  +  PRCG  D                 +F   G +     +Y+FFPG   W  
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 263 EKRHLTYGFIHDYPSKYVPQV----MRAFATWEANTHFTFSQAVGGQTADIVLSFERRDH 322
            +R LTY F  D  +    +V     RAF  WE  T  TF++     T+DI + F   +H
Sbjct: 171 NRRDLTYAF--DPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEH 230

Query: 323 GDGHPFDGLGGALAHCFGPIDGRVHFDAQEIW-------DWGIRILQN-DLESVALHEIG 382
           GDG PFDG    LAH F P  G  H D +E W       D  I + +  DLESVA+HEIG
Sbjct: 231 GDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIG 290

Query: 383 HALGLGHTDVQDAVMWPTMNFGSRKTRLHDDDINGIHALYG 394
           H LGLGH+ V+ ++M+PT+  G RK  L  DD+ G+  LYG
Sbjct: 291 HLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

BLAST of Cp4.1LG13g08880 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.4e-42
Identity = 97/250 (38.80%), Postives = 134/250 (53.60%), Query Frame = 0

Query: 156 VKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGALDKETLDQMAKPRC 215
           +K +LQ+YGY     E++      D + E+++  YQK   L ++G  D +TL Q+  PRC
Sbjct: 53  IKRHLQQYGYLPQNKESD------DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRC 112

Query: 216 GVSDNFTTAGGKL-GGSYYAFFPGRRIWAPE-KRHLTYGFIHDYPSKYVPQV------MR 275
           G  D+          G  Y +FPGR  W  +    LTY F  +  + Y+          R
Sbjct: 113 GFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRR 172

Query: 276 AFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDGRVHFD 335
           AF  W +    +F +      ADI + F   DHGDG PFDG+ G LAH F P +GR+H D
Sbjct: 173 AFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLD 232

Query: 336 AQEIW----DWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHDD 394
             E W    D     +  DLESVA+HEIGH LGLGH+ V+DA M+PT+   S+K  L+ D
Sbjct: 233 KAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMD 292

BLAST of Cp4.1LG13g08880 vs. NCBI nr
Match: XP_023551530.1 (metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538 bits (1385), Expect = 1.98e-190
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL
Sbjct: 28  GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 87

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYV 262
           DKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYV
Sbjct: 88  DKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYV 147

Query: 263 PQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDG 322
           PQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDG
Sbjct: 148 PQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDG 207

Query: 323 RVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 382
           RVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHD
Sbjct: 208 RVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 267

Query: 383 DDINGIHALYGN 394
           DDINGIHALYGN
Sbjct: 268 DDINGIHALYGN 279

BLAST of Cp4.1LG13g08880 vs. NCBI nr
Match: XP_022993363.1 (metalloendoproteinase 5-MMP-like [Cucurbita maxima])

HSP 1 Score: 417 bits (1073), Expect = 2.78e-143
Identity = 200/252 (79.37%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GS +GDNRKGIHGVKAYLQRYGYY+TQNETNSQTNAFDEALE SIKSYQKFFKLNVSGAL
Sbjct: 46  GSHRGDNRKGIHGVKAYLQRYGYYLTQNETNSQTNAFDEALEASIKSYQKFFKLNVSGAL 105

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYV 262
           DKETLDQMAKPRCG+SDNFT AGGKLGGSYYAFFPGR IW PEKRHLTYGFIH+YPSK  
Sbjct: 106 DKETLDQMAKPRCGLSDNFTMAGGKLGGSYYAFFPGRHIWPPEKRHLTYGFIHEYPSK-- 165

Query: 263 PQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDG 322
                                               DHGDGHPFDG GGALAHCFGPIDG
Sbjct: 166 ------------------------------------DHGDGHPFDGFGGALAHCFGPIDG 225

Query: 323 RVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 382
           RVHFDAQE+WDWG+RILQNDLESVALHEI HALGLGHTDVQDAVMWPTMNFGSRKTRLHD
Sbjct: 226 RVHFDAQEVWDWGVRILQNDLESVALHEIRHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 259

Query: 383 DDINGIHALYGN 394
           DDINGI ALYGN
Sbjct: 286 DDINGIRALYGN 259

BLAST of Cp4.1LG13g08880 vs. NCBI nr
Match: KAA0037452.1 (metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 326 bits (836), Expect = 1.17e-106
Identity = 163/262 (62.21%), Postives = 187/262 (71.37%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GSRK DN +GIH VKAYLQRYGY   + E N  T+AFD+ LE +IKSYQK FKLNVSG L
Sbjct: 42  GSRKNDNFEGIHKVKAYLQRYGYLSDEKENNLSTDAFDDDLESAIKSYQKVFKLNVSGVL 101

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTYG 262
           D+ETL QMA+PRCGV+D+F     +          +GGS+Y F+P +  W  EKRHLTYG
Sbjct: 102 DRETLQQMARPRCGVTDDFILVAQQDHENNNTNIEIGGSHYMFYPNKLKWPVEKRHLTYG 161

Query: 263 FIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGA 322
           FIH+YP ++   V+RAF TWEANT FTFS A   QTADI+LSFER DHGDG PFDG GGA
Sbjct: 162 FIHNYPPEHAAAVIRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGA 221

Query: 323 LAHCFGPIDGRVHFDAQEIWDWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWPTM 382
           L H FG IDGRVHFD  E W+ G    +  D+ESVALHEIGHALGLGH+    AVMW  M
Sbjct: 222 LGHAFGAIDGRVHFDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANM 281

Query: 383 NFGSRKTRLHDDDINGIHALYG 393
           + G  KT L  DDI GIHALYG
Sbjct: 282 DSGVTKTHLTIDDIEGIHALYG 303

BLAST of Cp4.1LG13g08880 vs. NCBI nr
Match: XP_008458911.1 (PREDICTED: metalloendoproteinase 3-MMP-like [Cucumis melo])

HSP 1 Score: 324 bits (830), Expect = 9.49e-106
Identity = 162/262 (61.83%), Postives = 186/262 (70.99%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GSRK DN +GIH VKAYLQRYGY   + E N   +AFD+ LE +IKSYQK FKLNVSG L
Sbjct: 42  GSRKNDNFEGIHKVKAYLQRYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVL 101

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTYG 262
           D+ETL QMA+PRCGV+D+F     +          +GGS+Y F+P +  W  EKRHLTYG
Sbjct: 102 DRETLQQMARPRCGVTDDFILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYG 161

Query: 263 FIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGA 322
           FIH+YP ++   V+RAF TWEANT FTFS A   QTADI+LSFER DHGDG PFDG GGA
Sbjct: 162 FIHNYPPEHAAAVIRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGA 221

Query: 323 LAHCFGPIDGRVHFDAQEIWDWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWPTM 382
           L H FG IDGRVHFD  E W+ G    +  D+ESVALHEIGHALGLGH+    AVMW  M
Sbjct: 222 LGHAFGAIDGRVHFDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANM 281

Query: 383 NFGSRKTRLHDDDINGIHALYG 393
           + G  KT L  DDI GIHALYG
Sbjct: 282 DSGVTKTHLTIDDIEGIHALYG 303

BLAST of Cp4.1LG13g08880 vs. NCBI nr
Match: XP_004142466.2 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52408.1 hypothetical protein Csa_008745 [Cucumis sativus])

HSP 1 Score: 303 bits (777), Expect = 1.04e-97
Identity = 155/262 (59.16%), Postives = 182/262 (69.47%), Query Frame = 0

Query: 143 GSRKGDNR-KGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGA 202
           GS K D+  +GIH VKAYLQRYGY+  +NE N  T+AFD+ LE +IKSYQKF  L VSG 
Sbjct: 42  GSSKNDSIVEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGV 101

Query: 203 LDKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTY 262
           LD+ETL QM++PRCGV+DNF +   +          +GGS++ FFPG+  W   K HLTY
Sbjct: 102 LDRETLQQMSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTY 161

Query: 263 GFIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGG 322
           GF+H+YP K+   V+RAF  W AN+ FTFS A   QTADI+LSFER DHGDG PFDG GG
Sbjct: 162 GFVHNYPMKHAAAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGG 221

Query: 323 ALAHCFGPIDGRVHFDAQEIW-DWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWP 382
            L H FGPIDGRVHFDA E W + G    +N D ESVALHEIGHALGLGH+    AVMW 
Sbjct: 222 ILGHAFGPIDGRVHFDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWA 281

Query: 383 TMNFGSRKTRLHDDDINGIHAL 391
            M  G  KT L  DDI G+HAL
Sbjct: 282 NMETGVNKTELTIDDIEGVHAL 303

BLAST of Cp4.1LG13g08880 vs. ExPASy TrEMBL
Match: A0A6J1K202 (metalloendoproteinase 5-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489405 PE=3 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 1.34e-143
Identity = 200/252 (79.37%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GS +GDNRKGIHGVKAYLQRYGYY+TQNETNSQTNAFDEALE SIKSYQKFFKLNVSGAL
Sbjct: 46  GSHRGDNRKGIHGVKAYLQRYGYYLTQNETNSQTNAFDEALEASIKSYQKFFKLNVSGAL 105

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYV 262
           DKETLDQMAKPRCG+SDNFT AGGKLGGSYYAFFPGR IW PEKRHLTYGFIH+YPSK  
Sbjct: 106 DKETLDQMAKPRCGLSDNFTMAGGKLGGSYYAFFPGRHIWPPEKRHLTYGFIHEYPSK-- 165

Query: 263 PQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDG 322
                                               DHGDGHPFDG GGALAHCFGPIDG
Sbjct: 166 ------------------------------------DHGDGHPFDGFGGALAHCFGPIDG 225

Query: 323 RVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 382
           RVHFDAQE+WDWG+RILQNDLESVALHEI HALGLGHTDVQDAVMWPTMNFGSRKTRLHD
Sbjct: 226 RVHFDAQEVWDWGVRILQNDLESVALHEIRHALGLGHTDVQDAVMWPTMNFGSRKTRLHD 259

Query: 383 DDINGIHALYGN 394
           DDINGI ALYGN
Sbjct: 286 DDINGIRALYGN 259

BLAST of Cp4.1LG13g08880 vs. ExPASy TrEMBL
Match: A0A5D3BUL7 (Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00140 PE=3 SV=1)

HSP 1 Score: 326 bits (836), Expect = 5.69e-107
Identity = 163/262 (62.21%), Postives = 187/262 (71.37%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GSRK DN +GIH VKAYLQRYGY   + E N  T+AFD+ LE +IKSYQK FKLNVSG L
Sbjct: 42  GSRKNDNFEGIHKVKAYLQRYGYLSDEKENNLSTDAFDDDLESAIKSYQKVFKLNVSGVL 101

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTYG 262
           D+ETL QMA+PRCGV+D+F     +          +GGS+Y F+P +  W  EKRHLTYG
Sbjct: 102 DRETLQQMARPRCGVTDDFILVAQQDHENNNTNIEIGGSHYMFYPNKLKWPVEKRHLTYG 161

Query: 263 FIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGA 322
           FIH+YP ++   V+RAF TWEANT FTFS A   QTADI+LSFER DHGDG PFDG GGA
Sbjct: 162 FIHNYPPEHAAAVIRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGA 221

Query: 323 LAHCFGPIDGRVHFDAQEIWDWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWPTM 382
           L H FG IDGRVHFD  E W+ G    +  D+ESVALHEIGHALGLGH+    AVMW  M
Sbjct: 222 LGHAFGAIDGRVHFDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANM 281

Query: 383 NFGSRKTRLHDDDINGIHALYG 393
           + G  KT L  DDI GIHALYG
Sbjct: 282 DSGVTKTHLTIDDIEGIHALYG 303

BLAST of Cp4.1LG13g08880 vs. ExPASy TrEMBL
Match: A0A1S3C8Z2 (metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV=1)

HSP 1 Score: 324 bits (830), Expect = 4.60e-106
Identity = 162/262 (61.83%), Postives = 186/262 (70.99%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           GSRK DN +GIH VKAYLQRYGY   + E N   +AFD+ LE +IKSYQK FKLNVSG L
Sbjct: 42  GSRKNDNFEGIHKVKAYLQRYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVL 101

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTYG 262
           D+ETL QMA+PRCGV+D+F     +          +GGS+Y F+P +  W  EKRHLTYG
Sbjct: 102 DRETLQQMARPRCGVTDDFILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYG 161

Query: 263 FIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGA 322
           FIH+YP ++   V+RAF TWEANT FTFS A   QTADI+LSFER DHGDG PFDG GGA
Sbjct: 162 FIHNYPPEHAAAVIRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGA 221

Query: 323 LAHCFGPIDGRVHFDAQEIWDWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWPTM 382
           L H FG IDGRVHFD  E W+ G    +  D+ESVALHEIGHALGLGH+    AVMW  M
Sbjct: 222 LGHAFGAIDGRVHFDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANM 281

Query: 383 NFGSRKTRLHDDDINGIHALYG 393
           + G  KT L  DDI GIHALYG
Sbjct: 282 DSGVTKTHLTIDDIEGIHALYG 303

BLAST of Cp4.1LG13g08880 vs. ExPASy TrEMBL
Match: A0A0A0KS35 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 303 bits (777), Expect = 5.04e-98
Identity = 155/262 (59.16%), Postives = 182/262 (69.47%), Query Frame = 0

Query: 143 GSRKGDNR-KGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGA 202
           GS K D+  +GIH VKAYLQRYGY+  +NE N  T+AFD+ LE +IKSYQKF  L VSG 
Sbjct: 42  GSSKNDSIVEGIHKVKAYLQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGV 101

Query: 203 LDKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTY 262
           LD+ETL QM++PRCGV+DNF +   +          +GGS++ FFPG+  W   K HLTY
Sbjct: 102 LDRETLQQMSRPRCGVADNFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTY 161

Query: 263 GFIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGG 322
           GF+H+YP K+   V+RAF  W AN+ FTFS A   QTADI+LSFER DHGDG PFDG GG
Sbjct: 162 GFVHNYPMKHAAAVVRAFDKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGG 221

Query: 323 ALAHCFGPIDGRVHFDAQEIW-DWGIRILQN-DLESVALHEIGHALGLGHTDVQDAVMWP 382
            L H FGPIDGRVHFDA E W + G    +N D ESVALHEIGHALGLGH+    AVMW 
Sbjct: 222 ILGHAFGPIDGRVHFDADEQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWA 281

Query: 383 TMNFGSRKTRLHDDDINGIHAL 391
            M  G  KT L  DDI G+HAL
Sbjct: 282 NMETGVNKTELTIDDIEGVHAL 303

BLAST of Cp4.1LG13g08880 vs. ExPASy TrEMBL
Match: A0A5D3BRA5 (Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00150 PE=3 SV=1)

HSP 1 Score: 295 bits (754), Expect = 1.40e-94
Identity = 148/262 (56.49%), Postives = 180/262 (68.70%), Query Frame = 0

Query: 143 GSRKGDNR-KGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGA 202
           GS K D+  +GIH VKAYLQRYGY   +NE N  T+AFD+ LE +IKSYQKF K+ VSG 
Sbjct: 42  GSSKNDSIVEGIHKVKAYLQRYGYLSNENENNLSTDAFDDDLESAIKSYQKFSKIKVSGV 101

Query: 203 LDKETLDQMAKPRCGVSDNFTTAGGK----------LGGSYYAFFPGRRIWAPEKRHLTY 262
           LD+ETL QM+ PRCGV D F +   +          +GGS+Y F+     W  EK+HL+Y
Sbjct: 102 LDRETLQQMSTPRCGVRDTFVSVAQQEHENKSTNIEIGGSHYTFYYNNVKWPAEKKHLSY 161

Query: 263 GFIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGG 322
           GFIH++P ++VP V+RAF TWE NT F+F  A   QTADI++SFER +HGD  PFDG GG
Sbjct: 162 GFIHNFPPQHVPTVLRAFQTWEDNTKFSFFLAPRVQTADILVSFERGEHGDDEPFDGPGG 221

Query: 323 ALAHCFGPIDGRVHFDAQEIWDWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTM 382
            LAH  G +DGRVHFDA E W+ G    + DLE++ALHE+GHALGLGH+    AVMW  M
Sbjct: 222 ILAHSLGAVDGRVHFDADEQWEGGPVEKRYDLETLALHELGHALGLGHSSSSLAVMWAYM 281

Query: 383 NFGSRKTRLHDDDINGIHALYG 393
             G  KTRL  DDI GI ALYG
Sbjct: 282 YAGFPKTRLTIDDIEGIRALYG 303

BLAST of Cp4.1LG13g08880 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.0e-51
Identity = 113/268 (42.16%), Postives = 145/268 (54.10%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G+N  G+  +K Y +R+GY  T   T + T+ FD+ L+ +I +YQK F L V+G L
Sbjct: 50  GCHIGENINGLSKLKQYFRRFGYITT---TGNCTDDFDDVLQSAINTYQKNFNLKVTGKL 109

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGGSY------YAFFPGRRIWAPEKRHLTYGFI-- 262
           D  TL Q+ KPRCG  D         GG        Y+FFPG+  W   KR LTY F   
Sbjct: 110 DSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQ 169

Query: 263 HDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALA 322
           ++   +      RAF  W   T   F+++     ADIV+ F   +HGDG PFDG  G LA
Sbjct: 170 NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLA 229

Query: 323 HCFGPIDGRVHFDAQEIW-----DWGIRILQ----NDLESVALHEIGHALGLGHTDVQDA 382
           H   P  G +H D  E W     +   RIL      DLESVA+HEIGH LGLGH+ V+DA
Sbjct: 230 HASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDA 289

Query: 383 VMWPTMNFGSRKTRLHDDDINGIHALYG 394
           +M+P ++ G RK  L  DDI GI  LYG
Sbjct: 290 IMFPAISGGDRKVELAKDDIEGIQHLYG 314

BLAST of Cp4.1LG13g08880 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 188.0 bits (476), Expect = 1.5e-47
Identity = 107/277 (38.63%), Postives = 145/277 (52.35%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G N  G++ +K Y QR+G Y+ +  + + T+ FD+ L+ +++ YQ  F LNV+G L
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFG-YIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGEL 108

Query: 203 DKETLDQMAKPRCGVSDNFTTAGGKLGG-----------------SYYAFFPGRRIWAPE 262
           D  T+  +  PRCG  D         GG                   Y  FPG   W   
Sbjct: 109 DALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRN 168

Query: 263 KRHLTYGFIHDYP-SKYVPQVM-RAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDG 322
           +R LTY F    P ++ V  V  RAF  W   T   F+ +    T+DI + F   DHGDG
Sbjct: 169 RRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDG 228

Query: 323 HPFDGLGGALAHCFGPIDGRVHFDAQEIW------DWGIRILQN-DLESVALHEIGHALG 382
            PFDG+ G LAH F P  G+ H DA E W      D  + +    DLESVA+HEIGH LG
Sbjct: 229 EPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLG 288

Query: 383 LGHTDVQDAVMWPTMNFGSRKTRLHDDDINGIHALYG 394
           LGH+ V++++M+PT+  G RK  L +DD+ GI  LYG
Sbjct: 289 LGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

BLAST of Cp4.1LG13g08880 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 186.4 bits (472), Expect = 4.3e-47
Identity = 106/281 (37.72%), Postives = 147/281 (52.31%), Query Frame = 0

Query: 143 GSRKGDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGAL 202
           G   G    G++ +K Y Q +GY    N + + T+ FD+ L+ +++ YQ+ F+LNV+G L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 203 DKETLDQMAKPRCGVSD-----------------NFTTAGGKLGG-SYYAFFPGRRIWAP 262
           D+ TL  +  PRCG  D                 +F   G +     +Y+FFPG   W  
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 263 EKRHLTYGFIHDYPSKYVPQV----MRAFATWEANTHFTFSQAVGGQTADIVLSFERRDH 322
            +R LTY F  D  +    +V     RAF  WE  T  TF++     T+DI + F   +H
Sbjct: 171 NRRDLTYAF--DPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEH 230

Query: 323 GDGHPFDGLGGALAHCFGPIDGRVHFDAQEIW-------DWGIRILQN-DLESVALHEIG 382
           GDG PFDG    LAH F P  G  H D +E W       D  I + +  DLESVA+HEIG
Sbjct: 231 GDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIG 290

Query: 383 HALGLGHTDVQDAVMWPTMNFGSRKTRLHDDDINGIHALYG 394
           H LGLGH+ V+ ++M+PT+  G RK  L  DD+ G+  LYG
Sbjct: 291 HLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

BLAST of Cp4.1LG13g08880 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 173.3 bits (438), Expect = 3.8e-43
Identity = 97/250 (38.80%), Postives = 134/250 (53.60%), Query Frame = 0

Query: 156 VKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGALDKETLDQMAKPRC 215
           +K +LQ+YGY     E++      D + E+++  YQK   L ++G  D +TL Q+  PRC
Sbjct: 53  IKRHLQQYGYLPQNKESD------DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRC 112

Query: 216 GVSDNFTTAGGKL-GGSYYAFFPGRRIWAPE-KRHLTYGFIHDYPSKYVPQV------MR 275
           G  D+          G  Y +FPGR  W  +    LTY F  +  + Y+          R
Sbjct: 113 GFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRR 172

Query: 276 AFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFGPIDGRVHFD 335
           AF  W +    +F +      ADI + F   DHGDG PFDG+ G LAH F P +GR+H D
Sbjct: 173 AFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLD 232

Query: 336 AQEIW----DWGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNFGSRKTRLHDD 394
             E W    D     +  DLESVA+HEIGH LGLGH+ V+DA M+PT+   S+K  L+ D
Sbjct: 233 KAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMD 292

BLAST of Cp4.1LG13g08880 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 169.1 bits (427), Expect = 7.2e-42
Identity = 99/260 (38.08%), Postives = 137/260 (52.69%), Query Frame = 0

Query: 147 GDNRKGIHGVKAYLQRYGYYVTQNETNSQTNAFDEALEESIKSYQKFFKLNVSGALDKET 206
           G +  G+  +K YL R+GY    +E  S  + FD  LE +I  YQ+   L ++G LD  T
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS--DVFDGPLESAISLYQENLGLPITGRLDTST 123

Query: 207 LDQMAKPRCGVSDNFTTAGGKL--GGSYYAFFPGRRIWAPEKRHLTYGFIHDYPSKYVPQ 266
           +  M+ PRCGVSD   T         ++Y +F G+  W   +  LTY     +   Y+  
Sbjct: 124 VTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTS 183

Query: 267 ------VMRAFATWEANTHFTFSQAVGGQTADIVLSFERRDHGDGHPFDGLGGALAHCFG 326
                   RAF+ W +    +F +     TAD+ + F   DHGDG PFDG+ G LAH F 
Sbjct: 184 EDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFA 243

Query: 327 PIDGRVHFDAQEIWD-----WGIRILQNDLESVALHEIGHALGLGHTDVQDAVMWPTMNF 386
           P +GR+H DA E W       G   +  DLESVA HEIGH LGLGH+  + AVM+P++  
Sbjct: 244 PENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRP 303

Query: 387 GSRKTRLHDDDINGIHALYG 394
            ++K  L  DD+ G+  LYG
Sbjct: 304 RTKKVDLTVDDVAGVLKLYG 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUJ57.0e-5042.16Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O045292.1e-4638.63Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
P291364.7e-4641.15Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Q5XF516.1e-4637.72Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q8GWW65.4e-4238.80Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023551530.11.98e-190100.00metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo][more]
XP_022993363.12.78e-14379.37metalloendoproteinase 5-MMP-like [Cucurbita maxima][more]
KAA0037452.11.17e-10662.21metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloe... [more]
XP_008458911.19.49e-10661.83PREDICTED: metalloendoproteinase 3-MMP-like [Cucumis melo][more]
XP_004142466.21.04e-9759.16metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52408.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
A0A6J1K2021.34e-14379.37metalloendoproteinase 5-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489405 PE=... [more]
A0A5D3BUL75.69e-10762.21Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C8Z24.60e-10661.83metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV... [more]
A0A0A0KS355.04e-9859.16ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632070 PE=3 S... [more]
A0A5D3BRA51.40e-9456.49Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT1G59970.15.0e-5142.16Matrixin family protein [more]
AT1G70170.11.5e-4738.63matrix metalloproteinase [more]
AT1G24140.14.3e-4737.72Matrixin family protein [more]
AT2G45040.13.8e-4338.80Matrixin family protein [more]
AT4G16640.17.2e-4238.08Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 380..393
score: 60.98
coord: 210..223
score: 51.31
coord: 265..280
score: 36.28
coord: 346..371
score: 53.42
coord: 289..317
score: 56.73
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 238..394
e-value: 8.4E-25
score: 98.4
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 139..394
e-value: 6.6E-74
score: 250.4
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 1..118
e-value: 2.0E-7
score: 32.6
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 155..210
e-value: 4.9E-13
score: 49.1
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 246..393
e-value: 7.5E-41
score: 139.6
NoneNo IPR availablePANTHERPTHR10201:SF213MATRIX METALLOPROTEINASEcoord: 5..115
coord: 143..392
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 143..392
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 5..115
coord: 107..139
NoneNo IPR availablePANTHERPTHR10201:SF213MATRIX METALLOPROTEINASEcoord: 107..139
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 46..135
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 235..393
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 245..393
e-value: 1.94392E-55
score: 177.781
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 147..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g08880.1Cp4.1LG13g08880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity