Cp4.1LG12g11800 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g11800
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein ROOT PRIMORDIUM DEFECTIVE 1
LocationCp4.1LG12: 8469360 .. 8470412 (+)
RNA-Seq ExpressionCp4.1LG12g11800
SyntenyCp4.1LG12g11800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTCTACGCCCTATGCGCTCTTCTCATGTCTGCGCAGCTTCAAATCTGCTCAGTCTTCTTTTGTGAGGCGAAAATTCGTCTTACAGATCAGAGACGTTTCGAGTTTGAAGGTTATTTGGCGCAAGGATTCCAGATTGGACGAAGCCATTGAGAACGACAAGCGGTACAAGCTATGTGCGCGGGTTGTGAGGGAAGTTCTCAACGAGCCTGGACAGGTGATTCCGCTTCGATATCTGGAGAAACGACGGCAGAGATTGCGTCTCAATGTGTCAATCAAGACGTTTATGAGTTTTAATCCGGGTTTGTTGGATACTTACTATGATAGAATCAAACCTAAATCTGAACCCGTCCTGTTCTTACGCCCGAGCGACCGGCTTAGGGGTTTTTTGGTCAATTTGGTGCCGAAATACCCTGAATATTTTCAGATAATTGGTAGTCCTGGAGACGAAAAATCGTTTCTTCAATTGGTTTCTCGAAACCCTAACTTTGCGAAATCAGTGATTGAGAGAAGAGCCGAGGACGAATCCATATCGACAGGCATTGATATTCGACCCAATTTCAACTACAATCTTCCTCCTGGATTCTTCTTAAGAAAGGAAATGAGAGAATGGGTTAGAGATTGGTTAGAACTAGATTACATATCGCCGTACGAGGACGTGTCCCATTTGGCCCAAGCTTCGCCGGAGATGGAGAAGAGAACAGTAGGAGTTTTTCACGAGTTGCTATCACTTTCATTGTTCAAGAGGATTCCAGTGCCGATATTGGGAAAATTTAGTGATGAGTACCGGTTTTCAAATGCATTTTCCAGTGTGTTCACAAGGCATTCTGGGATATTTTATTTGTCTTTGAAAGGTGGGATTGAGACTGCGATGCTTCGAGAGGCATATAAGGGTAGCCAGTTGATTGATTGTGACCCATTGCTTGAAATAAAAGATAAGTTCGTGGAGTTGTTGGAAGAAGGATGGAGGGAGAGAGCTGAGCAGCGAAGGCAGCAGGCTAAACAAGTGAGGAACGACATGGAATTCATGGCTGCAAGAGCTGACGCTTAA

mRNA sequence

ATGCTCTCTACGCCCTATGCGCTCTTCTCATGTCTGCGCAGCTTCAAATCTGCTCAGTCTTCTTTTGTGAGGCGAAAATTCGTCTTACAGATCAGAGACGTTTCGAGTTTGAAGGTTATTTGGCGCAAGGATTCCAGATTGGACGAAGCCATTGAGAACGACAAGCGGTACAAGCTATGTGCGCGGGTTGTGAGGGAAGTTCTCAACGAGCCTGGACAGGTGATTCCGCTTCGATATCTGGAGAAACGACGGCAGAGATTGCGTCTCAATGTGTCAATCAAGACGTTTATGAGTTTTAATCCGGGTTTGTTGGATACTTACTATGATAGAATCAAACCTAAATCTGAACCCGTCCTGTTCTTACGCCCGAGCGACCGGCTTAGGGGTTTTTTGGTCAATTTGGTGCCGAAATACCCTGAATATTTTCAGATAATTGGTAGTCCTGGAGACGAAAAATCGTTTCTTCAATTGGTTTCTCGAAACCCTAACTTTGCGAAATCAGTGATTGAGAGAAGAGCCGAGGACGAATCCATATCGACAGGCATTGATATTCGACCCAATTTCAACTACAATCTTCCTCCTGGATTCTTCTTAAGAAAGGAAATGAGAGAATGGGTTAGAGATTGGTTAGAACTAGATTACATATCGCCGTACGAGGACGTGTCCCATTTGGCCCAAGCTTCGCCGGAGATGGAGAAGAGAACAGTAGGAGTTTTTCACGAGTTGCTATCACTTTCATTGTTCAAGAGGATTCCAGTGCCGATATTGGGAAAATTTAGTGATGAGTACCGGTTTTCAAATGCATTTTCCAGTGTGTTCACAAGGCATTCTGGGATATTTTATTTGTCTTTGAAAGGTGGGATTGAGACTGCGATGCTTCGAGAGGCATATAAGGGTAGCCAGTTGATTGATTGTGACCCATTGCTTGAAATAAAAGATAAGTTCGTGGAGTTGTTGGAAGAAGGATGGAGGGAGAGAGCTGAGCAGCGAAGGCAGCAGGCTAAACAAGTGAGGAACGACATGGAATTCATGGCTGCAAGAGCTGACGCTTAA

Coding sequence (CDS)

ATGCTCTCTACGCCCTATGCGCTCTTCTCATGTCTGCGCAGCTTCAAATCTGCTCAGTCTTCTTTTGTGAGGCGAAAATTCGTCTTACAGATCAGAGACGTTTCGAGTTTGAAGGTTATTTGGCGCAAGGATTCCAGATTGGACGAAGCCATTGAGAACGACAAGCGGTACAAGCTATGTGCGCGGGTTGTGAGGGAAGTTCTCAACGAGCCTGGACAGGTGATTCCGCTTCGATATCTGGAGAAACGACGGCAGAGATTGCGTCTCAATGTGTCAATCAAGACGTTTATGAGTTTTAATCCGGGTTTGTTGGATACTTACTATGATAGAATCAAACCTAAATCTGAACCCGTCCTGTTCTTACGCCCGAGCGACCGGCTTAGGGGTTTTTTGGTCAATTTGGTGCCGAAATACCCTGAATATTTTCAGATAATTGGTAGTCCTGGAGACGAAAAATCGTTTCTTCAATTGGTTTCTCGAAACCCTAACTTTGCGAAATCAGTGATTGAGAGAAGAGCCGAGGACGAATCCATATCGACAGGCATTGATATTCGACCCAATTTCAACTACAATCTTCCTCCTGGATTCTTCTTAAGAAAGGAAATGAGAGAATGGGTTAGAGATTGGTTAGAACTAGATTACATATCGCCGTACGAGGACGTGTCCCATTTGGCCCAAGCTTCGCCGGAGATGGAGAAGAGAACAGTAGGAGTTTTTCACGAGTTGCTATCACTTTCATTGTTCAAGAGGATTCCAGTGCCGATATTGGGAAAATTTAGTGATGAGTACCGGTTTTCAAATGCATTTTCCAGTGTGTTCACAAGGCATTCTGGGATATTTTATTTGTCTTTGAAAGGTGGGATTGAGACTGCGATGCTTCGAGAGGCATATAAGGGTAGCCAGTTGATTGATTGTGACCCATTGCTTGAAATAAAAGATAAGTTCGTGGAGTTGTTGGAAGAAGGATGGAGGGAGAGAGCTGAGCAGCGAAGGCAGCAGGCTAAACAAGTGAGGAACGACATGGAATTCATGGCTGCAAGAGCTGACGCTTAA

Protein sequence

MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLFLRPSDRLRGFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEIKDKFVELLEEGWRERAEQRRQQAKQVRNDMEFMAARADA
Homology
BLAST of Cp4.1LG12g11800 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.0e-32
Identity = 101/331 (30.51%), Postives = 149/331 (45.02%), Query Frame = 0

Query: 42  RKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIK--TFMSF 101
           RK+   D  ++ DK+ KL   + + ++++P +++ LR L K R+ L L    +    +  
Sbjct: 79  RKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKRRRFIALLRK 138

Query: 102 NPGLLD-----TYYDRIKPKSEPVLFLRPSDRLRG------------------------- 161
            PG+ +      Y  R K  SE         R+R                          
Sbjct: 139 YPGVFEIVEEGAYSLRFKMTSEAERLYLDEMRIRNELEDVLVVKLRKLVMMSIDKRILLE 198

Query: 162 --------------FLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIE----- 221
                         F   +  +YP+YF+++ +P      L+L   +P  A S  E     
Sbjct: 199 KISHLKTDLGLPLEFRDTICQRYPQYFRVVPTP--RGPALELTHWDPELAVSAAELSEDD 258

Query: 222 -RRAEDESISTGIDIRPNFN-YNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQAS 281
            R  E E  +  ID  P FN   LP G  L K     +  + ++ YISPY+D SHL   +
Sbjct: 259 NRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRDMQYISPYKDFSHLRSGT 318

Query: 282 PEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGI 320
            E EK   GV HELLSL+  KR  V  L  F +E+RFS     +  RH  +FY+SLKG  
Sbjct: 319 LEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDLFYVSLKGER 378

BLAST of Cp4.1LG12g11800 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.1e-30
Identity = 103/339 (30.38%), Postives = 152/339 (44.84%), Query Frame = 0

Query: 34  VSSLKVIWRKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRL------ 93
           V+   V  RK+   D  I+ DK+ KL  ++   +++ P +V+ LR L + R+ L      
Sbjct: 56  VAQAAVKRRKEIPFDNVIQRDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKR 115

Query: 94  RLNVSIKTF---------------MSFNPGLLDTYYDRIKPKSE----------PVLFLR 153
           RL   +K F                   P     Y D +  K+E           +L + 
Sbjct: 116 RLIALLKRFPGVFEVVEEGVYSLRFRLTPAAERLYLDELHLKNESEGLAVTKLRKLLMMS 175

Query: 154 PSDRL---------------RGFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKS 213
              R+                 F   +  +YP+YF+++    D    L+L   +P  A S
Sbjct: 176 QDKRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQM--DRGPGLELTHWDPELAVS 235

Query: 214 VIE------RRAEDESISTGIDIRPNFN-YNLPPGFFLRKEMREWVRDWLELDYISPYED 273
             E      R  E++  +  ID    FN   LP G  L +     V  + E+ YISPY D
Sbjct: 236 AAEVAEEENRAREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMPYISPYSD 295

Query: 274 VSHLAQASPEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIF 320
            SHL   S E EK   GV HE+LSL+L KR  V  L  F +E+RFS +   +  RH  +F
Sbjct: 296 FSHLRSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGMLIRHPDMF 355

BLAST of Cp4.1LG12g11800 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.0e-28
Identity = 102/339 (30.09%), Postives = 153/339 (45.13%), Query Frame = 0

Query: 34  VSSLKVIWRKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSI 93
           V+   V  RK++  D  I+ DK+ KL  ++   ++ +P +V+ LR L + R+ L L    
Sbjct: 50  VAQAAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKR 109

Query: 94  KTFMSFN--PGLLDTYYD-------RIKPKSEPVLF--LRPSDRLRGFLV---------- 153
           +        PG+ D   +       R+ P +E +    LR  +   G  V          
Sbjct: 110 RLIALLRRFPGVFDVVEEGVYSLRFRLTPAAERLYLDELRLRNESEGLAVAKLRKLLMMS 169

Query: 154 -----------------NLVP--------KYPEYFQIIGSPGDEKSFLQLVSRNPNFAKS 213
                             L P        +YP+YF+++    D    L+L   +P  A S
Sbjct: 170 QEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRM--DRGPALELTHWDPELAVS 229

Query: 214 VIE------RRAEDESISTGIDIRPNFN-YNLPPGFFLRKEMREWVRDWLELDYISPYED 273
             E      R  E E  +  ID    FN   LP G  L +     +  + E+ YISPY D
Sbjct: 230 AAELAEEESRAREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMPYISPYAD 289

Query: 274 VSHLAQASPEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIF 320
            SHL   S E EK   GV HE+LSL++ KR  V  L  F +E+RFS +   +  RH  +F
Sbjct: 290 FSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGMIIRHPDMF 349

BLAST of Cp4.1LG12g11800 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 65/200 (32.50%), Postives = 106/200 (53.00%), Query Frame = 0

Query: 130 FLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGI---DIRP 189
           F  +++ K+P++F++I        ++++V ++PN +   IER  E E  + GI   D+R 
Sbjct: 183 FEYSVILKHPQFFRLIDGEETRDKYIEIVEKDPNLSICAIERVREIEYRTKGIDAEDVRF 242

Query: 190 NFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVS----HLAQASPEMEKRTVGVFHEL 249
           +F  N PPGF + K  R  V  W  L Y SPYED+S       +A   +EKR+V   HEL
Sbjct: 243 SFVVNFPPGFKIGKYFRIAVWKWQRLPYWSPYEDISGYDLRSMEAQNRLEKRSVACIHEL 302

Query: 250 LSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGG---IETAMLREAYKG 309
           LSL++ K+I +  +  F +             +H GIFY+S +G    + T  LRE YK 
Sbjct: 303 LSLTVEKKITLERIAHFRNVMNLPKKLKEFLLQHQGIFYISTRGNYGKLHTVFLREGYKR 362

Query: 310 SQLIDCDPLLEIKDKFVELL 320
            +L++ + +   + +  EL+
Sbjct: 363 GELVEPNDVYLARRRLAELV 382

BLAST of Cp4.1LG12g11800 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.5e-15
Identity = 43/126 (34.13%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 195 GFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRIPVP 254
           G  LR ++ +W+ ++ +L Y+SPY+D S L  +S   EKR VG  HELL L +       
Sbjct: 231 GCRLRVKIEDWLMEFQKLPYVSPYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERK 290

Query: 255 ILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEIKDK 314
            L      +         F RH  IFYLS+K    TA+LRE Y+    ++  P+L ++ K
Sbjct: 291 KLLCLKKHFGLPQKVHKAFERHPQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKK 350

Query: 315 FVELLE 321
           +++L++
Sbjct: 351 YIQLMK 356

BLAST of Cp4.1LG12g11800 vs. NCBI nr
Match: XP_023548908.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo] >XP_023548909.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo] >XP_023548910.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693 bits (1788), Expect = 1.94e-251
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESIST 180
           LRPSDRLRGFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESIST
Sbjct: 121 LRPSDRLRGFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESIST 180

Query: 181 GIDIRPNFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFH 240
           GIDIRPNFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFH
Sbjct: 181 GIDIRPNFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFH 240

Query: 241 ELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGS 300
           ELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGS
Sbjct: 241 ELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGS 300

Query: 301 QLIDCDPLLEIKDKFVELLEEGWRERAEQRRQQAKQVRNDMEFMAARADA 350
           QLIDCDPLLEIKDKFVELLEEGWRERAEQRRQQAKQVRNDMEFMAARADA
Sbjct: 301 QLIDCDPLLEIKDKFVELLEEGWRERAEQRRQQAKQVRNDMEFMAARADA 350

BLAST of Cp4.1LG12g11800 vs. NCBI nr
Match: XP_022953521.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022953522.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022953524.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022953525.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022953526.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata])

HSP 1 Score: 657 bits (1696), Expect = 1.27e-236
Identity = 345/399 (86.47%), Postives = 347/399 (86.97%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLSTPYALFS LRSFKSAQS FVRRKFVLQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSTPYALFSYLRSFKSAQSCFVRRKFVLQIRSVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLL+TYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLNTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALIVSKLCKLLMISKDKVLSVDKLVQVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNP+FAKSVIERRAEDESISTGIDIRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPDFAKSVIERRAEDESISTGIDIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. NCBI nr
Match: KAG7014426.1 (Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 656 bits (1693), Expect = 3.63e-236
Identity = 345/399 (86.47%), Postives = 346/399 (86.72%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLS PYALFS LRSFKSAQS FVRRKFVLQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSKPYALFSYLRSFKSAQSCFVRRKFVLQIRAVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALIVSKLCKLLMISKDKVLSVDKLVQVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNP+FAKSVIERRAEDESISTGIDIRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPDFAKSVIERRAEDESISTGIDIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. NCBI nr
Match: KAG6575893.1 (Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655 bits (1689), Expect = 1.48e-235
Identity = 344/399 (86.22%), Postives = 345/399 (86.47%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLS PYALFS LRSFKSAQS FVRRKFVLQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSKPYALFSYLRSFKSAQSCFVRRKFVLQIRAVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALIVSKLCKLLMISKDKVLSVDKLVQVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNP+FAKSVIERRAEDESISTGIDIRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPDFAKSVIERRAEDESISTGIDIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPL EI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLFEI 360

BLAST of Cp4.1LG12g11800 vs. NCBI nr
Match: XP_022992526.1 (protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992527.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992528.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992529.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992530.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992531.1 protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima])

HSP 1 Score: 652 bits (1683), Expect = 1.21e-234
Identity = 343/399 (85.96%), Postives = 345/399 (86.47%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLSTPYALFS LRSFKSAQSSFVRRKF LQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSTPYALFSYLRSFKSAQSSFVRRKFFLQIRAVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLL+TY DRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLNTYCDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALLVSKLCKLLMISKDKLLSVDKLVHVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGI IRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIQIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVG+FHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGIFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. ExPASy TrEMBL
Match: A0A6J1GN70 (protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111456044 PE=4 SV=1)

HSP 1 Score: 657 bits (1696), Expect = 6.14e-237
Identity = 345/399 (86.47%), Postives = 347/399 (86.97%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLSTPYALFS LRSFKSAQS FVRRKFVLQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSTPYALFSYLRSFKSAQSCFVRRKFVLQIRSVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLL+TYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLNTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALIVSKLCKLLMISKDKVLSVDKLVQVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNP+FAKSVIERRAEDESISTGIDIRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPDFAKSVIERRAEDESISTGIDIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. ExPASy TrEMBL
Match: A0A6J1JZH3 (protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111488826 PE=4 SV=1)

HSP 1 Score: 652 bits (1683), Expect = 5.87e-235
Identity = 343/399 (85.96%), Postives = 345/399 (86.47%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           MLSTPYALFS LRSFKSAQSSFVRRKF LQIR VSSLKVIWRKDSRLDEAIENDKRYKLC
Sbjct: 1   MLSTPYALFSYLRSFKSAQSSFVRRKFFLQIRAVSSLKVIWRKDSRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLL+TY DRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLNTYCDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LRPSDRLRGFL                                                 
Sbjct: 121 LRPSDRLRGFLEEEKRITMENEALLVSKLCKLLMISKDKLLSVDKLVHVKREFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGI IRPNFNYN
Sbjct: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIQIRPNFNYN 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVG+FHELLSLSLFKRI
Sbjct: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGIFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 350
           PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI
Sbjct: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. ExPASy TrEMBL
Match: A0A0A0K559 (PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392430 PE=4 SV=1)

HSP 1 Score: 572 bits (1473), Expect = 6.22e-203
Identity = 295/397 (74.31%), Postives = 318/397 (80.10%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           M STP +L S LR  KS    F+ ++ ++Q R +SSLKV+WRKD RLDEAIENDKRYKLC
Sbjct: 1   MFSTPNSLISYLRGIKSHCPCFLSQEIIVQFRPISSLKVVWRKDYRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVV+EVLNEPGQVIPLRYLEKRR+RLRLNVS+K F+ FNPGL DTYYDRIKPKSEPVLF
Sbjct: 61  ARVVKEVLNEPGQVIPLRYLEKRRERLRLNVSVKAFLGFNPGLFDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LR SDRLRGFL                                                 
Sbjct: 121 LRASDRLRGFLEEEKRIMMENEELIVSKLCKLLMMSKDKMLSVDKLVHVKRDFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQI+G PG+ KSFLQLVSRNP+FAKSVIERRAEDESISTGI +RPNF+Y 
Sbjct: 181 VNLVPKYPEYFQIVGCPGEGKSFLQLVSRNPDFAKSVIERRAEDESISTGIRMRPNFDYK 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGF LRKEMREWVRDWLELDYISPYEDVSHLAQ+SPEMEKRTVGVFHELLSLSLFKRI
Sbjct: 241 LPPGFILRKEMREWVRDWLELDYISPYEDVSHLAQSSPEMEKRTVGVFHELLSLSLFKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 348
           PVPILGKF  EYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAY+G QLIDCDPLLEI
Sbjct: 301 PVPILGKFGGEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYEGGQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. ExPASy TrEMBL
Match: A0A1S3BS57 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103492691 PE=4 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 1.78e-202
Identity = 294/397 (74.06%), Postives = 319/397 (80.35%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           M STP++L S LR  KS   SF+ ++ ++Q R +SSLKV+WRKD RLDEAIENDKRYKLC
Sbjct: 1   MFSTPHSLISYLRRIKSPHPSFLSQEIIVQFRPISSLKVVWRKDYRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRR+RLRLNVS+K F+ FNPGLLDTYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRERLRLNVSVKAFLGFNPGLLDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLRGFL------------------------------------------------- 180
           LR SDRLRGFL                                                 
Sbjct: 121 LRASDRLRGFLEEEKRIMMENEELIVSKLCKLLMMSKDKMLSVDKLLHVKRDFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQI+GSPG+ KSFLQLV+RNP+FAKSVIERRAEDES STGI +RPNF+Y 
Sbjct: 181 VNLVPKYPEYFQIVGSPGEGKSFLQLVTRNPDFAKSVIERRAEDESRSTGIRMRPNFDYK 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGF LRKEMREWVRDWLELDYISPYEDVS+L Q+SPEMEKRTVGVFHELLSLSL KRI
Sbjct: 241 LPPGFILRKEMREWVRDWLELDYISPYEDVSYLEQSSPEMEKRTVGVFHELLSLSLLKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 348
           PVPILGKF DEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAY+G QLIDCDPLLEI
Sbjct: 301 PVPILGKFGDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYQGDQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. ExPASy TrEMBL
Match: A0A5D3DBW6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00130 PE=4 SV=1)

HSP 1 Score: 567 bits (1461), Expect = 4.17e-201
Identity = 292/397 (73.55%), Postives = 318/397 (80.10%), Query Frame = 0

Query: 1   MLSTPYALFSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLC 60
           M STP++L S LR  KS   SF+ ++ ++Q R +SSLKV+WRKD RLDEAIENDKRYKLC
Sbjct: 1   MFSTPHSLISYLRRIKSPHPSFLSQEIIVQFRPISSLKVVWRKDYRLDEAIENDKRYKLC 60

Query: 61  ARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLF 120
           ARVVREVLNEPGQVIPLRYLEKRR+RLRLNVS+K F+ FNPGLLDTYYDRIKPKSEPVLF
Sbjct: 61  ARVVREVLNEPGQVIPLRYLEKRRERLRLNVSVKAFLGFNPGLLDTYYDRIKPKSEPVLF 120

Query: 121 LRPSDRLR-------------------------------------------------GFL 180
           LR SDRLR                                                  FL
Sbjct: 121 LRASDRLRRFLEEEKRIMMENEELIVSKLCKLLMMSKDKMLSVDKLLHVKRDFGFPNDFL 180

Query: 181 VNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYN 240
           VNLVPKYPEYFQI+GSPG+ KSFLQLV+RNP+FAKSVIERRAEDES STGI +RPNF+Y 
Sbjct: 181 VNLVPKYPEYFQIVGSPGEGKSFLQLVTRNPDFAKSVIERRAEDESRSTGIRMRPNFDYK 240

Query: 241 LPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRI 300
           LPPGF LRKEMREWVRDWLELDY+SPYEDVS+L Q+SPEMEKRTVGVFHELLSLSL KRI
Sbjct: 241 LPPGFILRKEMREWVRDWLELDYLSPYEDVSYLEQSSPEMEKRTVGVFHELLSLSLLKRI 300

Query: 301 PVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEI 348
           PVPILGKF DEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAY+G QLIDCDPLLEI
Sbjct: 301 PVPILGKFGDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYQGDQLIDCDPLLEI 360

BLAST of Cp4.1LG12g11800 vs. TAIR 10
Match: AT3G63090.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 2.6e-128
Identity = 236/387 (60.98%), Postives = 275/387 (71.06%), Query Frame = 0

Query: 9   FSCLRSFKSAQSSFVRRKFVLQIRDVSSLKVIWRKDSRLDEAIENDKRYKLCARVVREVL 68
           +  ++S K A+         +QIR +SSLKV+W+KD+RLDEAIE DKRYKLCARVV+EVL
Sbjct: 10  YEIIKSLKHAK----EHSLGIQIRWISSLKVVWKKDTRLDEAIEQDKRYKLCARVVKEVL 69

Query: 69  NEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNPGLLDTYYDRIKPKSEPVLFLRPSDRLR 128
           NEPGQVIPLRYLEKRR+RLRL    K+F+  NP L + YYDRIKPKS+PV FLRP+ RLR
Sbjct: 70  NEPGQVIPLRYLEKRRERLRLTFKAKSFVEMNPSLFEIYYDRIKPKSDPVQFLRPTPRLR 129

Query: 129 G-------------------------------------------------FLVNLVPKYP 188
                                                             FL+ LV KYP
Sbjct: 130 AFLDEEQRIYSENEPLLVSKLCQLLMMAKDKVISADKLVHVKRDFGFPNDFLMKLVQKYP 189

Query: 189 EYFQIIGSPGDEKSFLQLVSRNPNFAKSVIERRAEDESISTGIDIRPNFNYNLPPGFFLR 248
            YF++ G P + KSFL+LVS NP+FAKS IE RAEDE++ TG+ IRPN+N  LP GFFLR
Sbjct: 190 NYFRLTGLPEEGKSFLELVSWNPDFAKSKIELRAEDETLKTGVRIRPNYNVKLPSGFFLR 249

Query: 249 KEMREWVRDWLELDYISPYEDVSHLAQASPEMEKRTVGVFHELLSLSLFKRIPVPILGKF 308
           KEMREW RDWLE DYISPYEDVSHL QAS EMEKRTVGV HELLSLSL KR+PVP LGKF
Sbjct: 250 KEMREWTRDWLEQDYISPYEDVSHLDQASKEMEKRTVGVVHELLSLSLLKRVPVPTLGKF 309

Query: 309 SDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLREAYKGSQLIDCDPLLEIKDKFVELL 347
            DE+RFSNAFSSVFTRHSGIFYLSLKGGI+TA+LREAYK  +L+D DPLL IKDKF+ LL
Sbjct: 310 CDEFRFSNAFSSVFTRHSGIFYLSLKGGIKTAVLREAYKDDELVDRDPLLAIKDKFLRLL 369

BLAST of Cp4.1LG12g11800 vs. TAIR 10
Match: AT2G31290.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 145.2 bits (365), Expect = 9.9e-35
Identity = 100/335 (29.85%), Postives = 162/335 (48.36%), Query Frame = 0

Query: 42  RKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNP 101
           +KD  L+ A+  +KR+ + +R+   +L  P QV  +++L+K+ + L L      ++   P
Sbjct: 29  KKDPDLESALSRNKRWIVNSRLKNIILRCPNQVASIKFLQKKFKTLDLQGKALNWLKKYP 88

Query: 102 GLL------DTYYDR--------------IKPKSEPV--------LFLRPSDRLR----- 161
                    D YY R              +K   EPV        L L  + RL      
Sbjct: 89  CCFHVYLENDEYYCRLTKPMMTLVEEEELVKDTQEPVLADRLAKLLMLSVNQRLNVVKLN 148

Query: 162 ----------GFLVNLVPKYPEYFQIIGSPGDEKSF-LQLVSRNPNFAKSVIERRAEDES 221
                      +++ +VPKY + F+++   G + S  ++L+   P  A S +E  A+   
Sbjct: 149 EFKRSFGFPDDYVIRIVPKYSDVFRLVNYSGRKSSMEIELLLWKPELAVSAVEAAAKK-- 208

Query: 222 ISTGIDIRPNFNYNLPPGFFLRKEMREWVR--DWLELDYISPYEDVSHLAQASPEMEKRT 281
                   P+F+ +LP  +      + W R  ++    YISPYED   L + S E EKR+
Sbjct: 209 ----CGSEPSFSCSLPSTW-----TKPWERFMEFNAFPYISPYEDHGDLVEGSKESEKRS 268

Query: 282 VGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLRE 331
           VG+ HELLSL+L+K++ +  L  F  E+      + +  +H GIFY+  K  + T +LRE
Sbjct: 269 VGLVHELLSLTLWKKLSIVKLSHFKREFGLPEKLNGMLLKHPGIFYVGNKYQVHTVILRE 328

BLAST of Cp4.1LG12g11800 vs. TAIR 10
Match: AT2G31290.2 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 145.2 bits (365), Expect = 9.9e-35
Identity = 100/335 (29.85%), Postives = 162/335 (48.36%), Query Frame = 0

Query: 42  RKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIKTFMSFNP 101
           +KD  L+ A+  +KR+ + +R+   +L  P QV  +++L+K+ + L L      ++   P
Sbjct: 29  KKDPDLESALSRNKRWIVNSRLKNIILRCPNQVASIKFLQKKFKTLDLQGKALNWLKKYP 88

Query: 102 GLL------DTYYDR--------------IKPKSEPV--------LFLRPSDRLR----- 161
                    D YY R              +K   EPV        L L  + RL      
Sbjct: 89  CCFHVYLENDEYYCRLTKPMMTLVEEEELVKDTQEPVLADRLAKLLMLSVNQRLNVVKLN 148

Query: 162 ----------GFLVNLVPKYPEYFQIIGSPGDEKSF-LQLVSRNPNFAKSVIERRAEDES 221
                      +++ +VPKY + F+++   G + S  ++L+   P  A S +E  A+   
Sbjct: 149 EFKRSFGFPDDYVIRIVPKYSDVFRLVNYSGRKSSMEIELLLWKPELAVSAVEAAAKK-- 208

Query: 222 ISTGIDIRPNFNYNLPPGFFLRKEMREWVR--DWLELDYISPYEDVSHLAQASPEMEKRT 281
                   P+F+ +LP  +      + W R  ++    YISPYED   L + S E EKR+
Sbjct: 209 ----CGSEPSFSCSLPSTW-----TKPWERFMEFNAFPYISPYEDHGDLVEGSKESEKRS 268

Query: 282 VGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGIETAMLRE 331
           VG+ HELLSL+L+K++ +  L  F  E+      + +  +H GIFY+  K  + T +LRE
Sbjct: 269 VGLVHELLSLTLWKKLSIVKLSHFKREFGLPEKLNGMLLKHPGIFYVGNKYQVHTVILRE 328

BLAST of Cp4.1LG12g11800 vs. TAIR 10
Match: AT4G01037.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-33
Identity = 101/331 (30.51%), Postives = 149/331 (45.02%), Query Frame = 0

Query: 42  RKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRLNVSIK--TFMSF 101
           RK+   D  ++ DK+ KL   + + ++++P +++ LR L K R+ L L    +    +  
Sbjct: 79  RKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKRRRFIALLRK 138

Query: 102 NPGLLD-----TYYDRIKPKSEPVLFLRPSDRLRG------------------------- 161
            PG+ +      Y  R K  SE         R+R                          
Sbjct: 139 YPGVFEIVEEGAYSLRFKMTSEAERLYLDEMRIRNELEDVLVVKLRKLVMMSIDKRILLE 198

Query: 162 --------------FLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFAKSVIE----- 221
                         F   +  +YP+YF+++ +P      L+L   +P  A S  E     
Sbjct: 199 KISHLKTDLGLPLEFRDTICQRYPQYFRVVPTP--RGPALELTHWDPELAVSAAELSEDD 258

Query: 222 -RRAEDESISTGIDIRPNFN-YNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLAQAS 281
            R  E E  +  ID  P FN   LP G  L K     +  + ++ YISPY+D SHL   +
Sbjct: 259 NRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRDMQYISPYKDFSHLRSGT 318

Query: 282 PEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLKGGI 320
            E EK   GV HELLSL+  KR  V  L  F +E+RFS     +  RH  +FY+SLKG  
Sbjct: 319 LEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRGMLIRHPDLFYVSLKGER 378

BLAST of Cp4.1LG12g11800 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 139.0 bits (349), Expect = 7.1e-33
Identity = 99/357 (27.73%), Postives = 159/357 (44.54%), Query Frame = 0

Query: 36  SLKVIWRKDSRLDEAIENDKRYKLCARVVREVLNEPGQVIPLRYLEKRRQRLRL--NVSI 95
           ++K+ W KD  LD  +  +K  +    +V  +   P   +P+  L   R +L L   + +
Sbjct: 34  NVKLKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLPHRGQLGLPQELKL 93

Query: 96  KTFMSFNPGL-----------------------LDTYYDRIKPK---------------- 155
             F+   P +                       +D YY+ +                   
Sbjct: 94  SAFIRRYPNIFVEHCYWDSAGTSVPCFGLTRETIDLYYEEVDVSRVNERDVLVRLCKLLM 153

Query: 156 --SEPVLFLRPSDRLR-------GFLVNLVPKYPEYFQIIGSPGDEKSFLQLVSRNPNFA 215
              E  L L   D LR        +  +L+ K+P+ F ++    D    L+L+  + + A
Sbjct: 154 LTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLVKLSSDLDG-LKLIHWDEHLA 213

Query: 216 KSVIERRAEDESISTGIDIRPNFNYNLPPGFFLRKEMREWVRDWLELDYISPYEDVSHLA 275
            S ++ R +      G D R  F      GF L+++  EW+++W  L Y SPY D SHL 
Sbjct: 214 VSQMQLRED-----VGNDERMAFPVKFTRGFGLKRKSIEWLQEWQRLPYTSPYVDASHLD 273

Query: 276 QASPEMEKRTVGVFHELLSLSLFKRIPVPILGKFSDEYRFSNAFSSVFTRHSGIFYLSLK 335
             +   EKR VGVFHELL L++ K+     +      +     F+ VF RH GIFY+S+K
Sbjct: 274 PRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFALPQKFTKVFERHPGIFYISMK 333

Query: 336 GGIETAMLREAYKGSQLIDCDPLLEIKDKFVELLEEGWRERAEQRRQQAKQVRNDME 343
              +T +LREAY    LI+  PL+E+++KF  ++ EG+ +R+  R    K V  D+E
Sbjct: 334 CDTQTVILREAYDRRHLIEKHPLVEVREKFANMMNEGFLDRS--RGLYQKSVEADLE 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0MFS52.0e-3230.51Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q65XL57.1e-3030.38Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japon... [more]
B6TTV81.0e-2830.09Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=... [more]
Q689D61.4e-2532.50Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1... [more]
Q9ZUZ66.5e-1534.13Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=W... [more]
Match NameE-valueIdentityDescription
XP_023548908.11.94e-251100.00protein WHAT'S THIS FACTOR 1 homolog [Cucurbita pepo subsp. pepo] >XP_023548909.... [more]
XP_022953521.11.27e-23686.47protein WHAT'S THIS FACTOR 1 homolog [Cucurbita moschata] >XP_022953522.1 protei... [more]
KAG7014426.13.63e-23686.47Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argy... [more]
KAG6575893.11.48e-23586.22Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperm... [more]
XP_022992526.11.21e-23485.96protein WHAT'S THIS FACTOR 1 homolog [Cucurbita maxima] >XP_022992527.1 protein ... [more]
Match NameE-valueIdentityDescription
A0A6J1GN706.14e-23786.47protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC1114560... [more]
A0A6J1JZH35.87e-23585.96protein WHAT'S THIS FACTOR 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111488826... [more]
A0A0A0K5596.22e-20374.31PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392430 PE=4 S... [more]
A0A1S3BS571.78e-20274.06protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103492691 PE=4... [more]
A0A5D3DBW64.17e-20173.55Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT3G63090.12.6e-12860.98Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT2G31290.19.9e-3529.85Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT2G31290.29.9e-3529.85Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT4G01037.11.4e-3330.51Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G48040.17.1e-3327.73Ubiquitin carboxyl-terminal hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 324..344
NoneNo IPR availablePANTHERPTHR31476:SF3UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEINcoord: 22..131
coord: 130..345
IPR021099Plant organelle RNA recognition domainPFAMPF11955PORRcoord: 43..113
e-value: 9.0E-10
score: 38.0
coord: 130..323
e-value: 2.6E-64
score: 217.2
IPR045040PORR familyPANTHERPTHR31476PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 130..345
coord: 22..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g11800.1Cp4.1LG12g11800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding