Cp4.1LG12g09530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g09530
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalanine aminotransferase 2
LocationCp4.1LG12: 5624713 .. 5630793 (+)
RNA-Seq ExpressionCp4.1LG12g09530
SyntenyCp4.1LG12g09530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGGAATGCGAGTATGCTGTTCGTGGTGAGATTGTCGCCCTCGCTCAGGTACCTTTACTGATTTTGATCTTTGCATGCTAATTAGAATGAATCCACTCTCGACGATGGTATGATCATTCTCTAAATTAACCGATGTGGGACTTTGGTCATCCAACACCTCCCTTCAAACAAAGTACGCCTCCCCTTAATCGAGCCTCGACTTCTTTGTAGTCTTAGTCATTTTTTACTATGCCTTCGAGGAGGGTTGACTACTTTTCTTTTGGAGTCTTTTGTTCGATATTTGAGGATTTACCAATCTATTGGCGTGACTAAGTTTAGGGCATAGATCTGATANATTGTAAATTCGGACGTTCCCCATTGTCATTCATTGTCCAAAAGTCATGCACTTATTTAATTAGGAACTAATGAAACTACATGTATCTATTCATATATGATATGGAAATCAACCCAAGAGAAAGTATGGAACGGATTACCTTGATGATCAAGATCAAGTAAAGAAAACTCGTTTCTAAACTCGTGATTCGAATCACTCCATAAGAGGTTTGATAAATACCACTTGAATGACTCTACATGCAAGTTCTAAACACAAGAATTTGCAAAGAAAGTTTAGCTCTCAACAAAGCTAAAATAGAAATTCTATTCTTAATTCCAAAATCTGATGTCTAATCAAAGAAAAGAAGACTTTATATAGCTTTTACAACCTAAACCTATGTCATACATAACTCCCAAATTAAATGGGCGAGTAAATGAGAAATACCCCTAGGTGGGTTACCGNTACCATGTTAGACGAACACGACTCTCAACAATGGTATGATATTCTCCACTTAGAGCATAAGCTCTCAAATTAACTAACCGAACAATCAGCAAGGTCCGGCAGAAAATCTAAGCAAATCTTGGCATTGAGTTGAGGGATTTAAGTATTACTTTGTTACCAATAAAGTTGAGATCTGAGAGCTGGTTTGGGCTTCTTTAATTAGCTCCAAAATTAATTGCTTAATTTGTGATAGTGCCATCCTTCTGCTTACTATCTTTTATCCTCTTAACTAACAGAGACTGGAAAGAGAGCTTCTGACTAATCCAGGCTCACATGCTTTTGATGAGGTTTGATTACTTTTCTCATCTTATTTCTTATTAGCATCTGGCACAGTTCATGCAACTCAATTCACTACCATTTAGAGTTTAGTTGAATTCAAATAAATTAAAAATTTATAGGTTTGGTCAATTCAATTAAAGTGTTTCTCCAAAATTAGTTAGATCGAATTGAAACAACCAAATTTATGGTTAATTTTAGTTTTTTTTTTCAACTATACATATACATGCATATATTTATTATAATTTTATAATTATTTTCTTGATTGTTTATTTATTTAACAACGATTAAAAATAAAATTTCAATTTTTAAATTGAGTTTGTCCATCTTGGATTGAGTTGATTATTTATTAGACTCCCTTGAATTGAAAATTTTAGGATNGAAAAAAAAAAAAAAAAAAAAAAAAAGTTTCATGAACTCCCCTGTTCAGGCTCCTCATTTAAGGCCATCATGGGAACGTAAACCTTTGGCTTATGCAAATTGCATGCAGCCAAAAACAGTGTCCTTTTTGAATGGTAGCGTAAGGTTTGTCTCATGCTACATGCTTAATAACACAATCTTGCTGGTGCTGATAGGTTTTTGTATGACCCAGTAACCATTACTCTATCACTTTATGATGCAGATACTGTACTGCAACATAGGAAATCCTCATTCCCTTGGTCAGCAACCAATAACATTTTTCCGTGAGGTTTGTCAGTGTACTGTATGTTGGCACTCTCCATTATACCTTGAGAAATTAAAATCCTGATTATCTTTGTTTATACAGGTTCTTGCACTGTGTGAGCATCCATCTCTTCTGGAAAGAAACGAAATAAACGGTTTGTTCAGGTACTGCAATTTAGTTAACTCATTCATGATGGAGACTGTTTAATCATGAAAAGAATTTATGTTTGAATACCCTGTTGCTTTCAAGAAAAGACTATTCCAGCTTCAGGAAAATATAATATCAGAGTACAAAATATAATATCAGCTTTAGATCGATTATGGTTTTGCTCAGATTATATATATCTNGATTGTCGCCCTCGCTCAGGTACCTTTACTGATTTTGATCTTTGCATGCTAATTAGAATGAATCCACTCTCCACGATGGTACGATCATTCCNTTTGTGCTGTTTCCTGCTTGATTTTTCTTAATAAACTTGTTGCAGTGAAGATGCCATTAAGCGAGCTTCGCAAATTCTGAAACAAATTCCTGGAAAAGCAACTGGTGCTTACAGCCATAGTCAGGTTGTGATCAGAAAAAAATGGAGTTCTAGGCTTCATAGAATTCAGCTCATCTAACATTTGATCTATTCGTCACACCAGGGAATCAAGGAGCTGCGAGATGCCATTGCTGAAGGAATTAAAGCTCGTGATGATTTTCCTGCAGATCCTAATGACATATTTCTAACAAATGGTGCAAGCCCAGCAGTGAGTTTTTTTTAATATATGTGACAACGAGTCTTTATATTTAGAAATGTTGACAGAAGATATTAACCTCAGAGTAATAGCATGTCAAAGAGGCTCTTTTATCTGTTTGTTATCAGGTCCATATGGTGATGCAATTGCTGATAAGTACAGAGAAAGATGGAATACTCTGCCCCATTCCTCAGTACCCTCTGTACTCTGCTTCTATAGCTCTTCATGGCGGTACTCTTGTACGTTTCGTATTATTTCAGAAAATTCTTATAGTTTGATTTGATTTCTTTGAGTTAACGTGTAATGATTTCAAGAAGTTGAAACTTATTGGTGGTTTGCAGGTTCCTTACTATCTTGATGAAGCATCGGGATGGGGATTGGAAATTTCTGAGCTGAAGAAGCAATTGGAGTCTGCCAAGTCTAAGGGTATTTCAGTAAGGGCATTGGCGATTATAAATCCCGGCAATCCTACTGGACAAGTATGTTATTGGATGGGTTTTGAATGTAAATCTTGGGATGAACTTATATTCTGGTTCCTACTTTAACATATGACTTTAGGTTCTCACTAAGGAAAACCAAGAACAAATAGTGAAGTTCTGCAAGCAAGAGGGCCTCGTTCTTCTGGCAGATGAGGTACATCTAGAACATTAATGGAAGCCTGCAAACATAACTTTATTGGATATATTAATTAGGTTTGGGTTCCTTGGATCAATACCAATATTGTTTAAGATCTGATGAACGGTGCAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGTTTGTTGTCTTAGTCCATATCTATTTTAGGCGTGGCAATGAGAATTGTGCAGAGATTAATGGTCTGATTTCATGCCTTACTTTTTTTTTTCTTTTCCTGGTATTAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGGTTAGAATCTTCTGATTTCTATGCATAACTAAATTCATCCCGATTCGGCTCTTTATAAGGCAAAAATGTCTGTTAGAAAGAGTTAAAGTTTAGTGCTTAACTTATTATCAATTCATTTTTCCTCGTTTGTTGAAGACTANCGGTGCAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGTTTGTTGTCTTAGTCCATATCTATTTTAGGCGTGGCAATGAGAATTGTGCAGAGATTAATGGTCTGATTTCATGCCTTACTTTTTTTTTTCTTTTCCTGGTATTAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGGTTAGAATCTTCTGATTTCTATGCATAACTAAATTCATCCCGATTCGGCTCTTTATAAGGCAAAAATGTCTGTTAGAAAGAGTTAAAGTTTAGTGCTTAACTTATTATCAATTCATTTTTCCTCGTTTGTTGAAGACTATTGATGATTTGTGCAGGTTGGGGATCACATCTATGAATCGTATTGTGCAGAGAGAGATGGAATCCTCTCATCCCTGGGCAGGCGTGCAAAGGTTTGTGGAATGAGAAAACTCTTTTTTATTATAGATATAAACATAGTAGGGAGTTAAAAAGTATATACATTAAACTAAAAGCATATATACCTCCATTTTTGTATGTGCTTTGAACATGTCTGTCGTCTATATTCTCTTAATACATAATTAAAGTCATCCATATAAACATTTAGCCAATATTTCTCTTTTTTTCTCTCACATGTTATTTCTCCTTTTTTCTTTCACCTGTTATTTGAGTTCATCTACCGTGTGCATTGTTGTGCAATACTAACATCCTTTTCCGTATTTAGTTGGTGGACTCTTATTTTTGAGTTTTTTTTTATATGCTCTTGCATATTCTTTCTTTTTCTCAATGAAAGCTCGGTTTCTCATCAAAAAATAATGGACTGTACTGCAGACATTAGAAGCTGCATTCAACAGTTTAGAAAATGTAACATGCAATAAAGCAGAAGGGGCAATGTACCTATTCCCTTGCATTAAGCTGCCTGAAAAGGCAATCAAAGCAGCAGAAGCAGCAGATACGACCCCGGATACTTTTTATTGCCGCCAACTTCTCAATGCCACTGGAGTGGTTGTCGTCCCCGGTTCTGGCTTTGGGCAGGTCTGTCTCATTGGCTTAAGCTTTCAATCTATTCCAAATCATCATCTGCAATATTTGAATTTTCCACCATTTATATCACACCAACATTTTTTTTTAGTTTTCAAAATTTAGCTTAGATTTTAAAAATGCGATTAGGAATCACGACACTCCACAATGGTATGATATGATATTATCCACTTTGAGCGTAAGCTCTCGTTGGCTTTGCTTTAAACTTCCCCAAAATCAATGGAGATGTATTCCTTACTTATAAATCCATGATCATTCCTTAAATTAGCCAGTGTGAGACTTCCTCCAAACAATCTTCATCCTCCCCACTAACAGAGTATACCATAGAGCCTCCCTTGAGGTCTATGTAGCCCTCAAAAAATCAATGGAGATGTATTCCTTACTTATAAATCCATGATCATTCCCTAAATTAGCGAGTGTGGGACTCCCTCCAAATAATCTTCAACAATCCTCCCCTCGAACAAAATATACCATAGAGTCTCCTCTGAGGACTATGTAGCCCTCGAATAGCCTCCCCTTAATCGAGGCTCGACTCCTTTCTCTGGAGCCCTCGAACAAAGTACACCCTCTGTTCGACACATGAGTCACTTCTGACTACACCTTCGAGGCTAGCGATTTCTTTGTTTGACACTTGATGATTCTATTGACATGGCTAACTTGAGCATGACTCTAATACCATGTTAGGAATCATGACTCTCTACAACGGTATGATATTGTCCACTTTGAGCATAAACTCTCAGGACTTAATTTTGAGTTTCCTCGAAAGGCCTTGTACAAATGGAGATGTATTCCTTGCTTATAAACCCATCATCATTTCCTAAATTAGCCAACGTGGGACTCCCTCCCAACAATGCTCATCAAAACGCTCATAAAAATTAAACATTGGGGGGAAGTGGGGCTTACCCGCTTAATTTTCAAAAACCAAAAATTGAAAAATGTAATCAAACCGGAACTAACCGATCCATTCTGCATCAGGTGGCCGGAACATGGCACTTCAGGTGTACAATACTTCCTCGAGAGAACAAGATCCCAGACGTCGTATCTCGCCTGACTGACTTCCATAAAGCATTCATGAACGAATATCGCGACTGA

mRNA sequence

GTTTTGGAATGCGAGTATGCTGTTCGTGGTGAGATTGTCGCCCTCGCTCAGAGACTGGAAAGAGAGCTTCTGACTAATCCAGGCTCACATGCTTTTGATGAGATACTGTACTGCAACATAGGAAATCCTCATTCCCTTGGTCAGCAACCAATAACATTTTTCCGTGAGGTTCTTGCACTGTGTGAGCATCCATCTCTTCTGGAAAGAAACGAAATAAACGGTTTGTTCAGTGAAGATGCCATTAAGCGAGCTTCGCAAATTCTGAAACAAATTCCTGGAAAAGCAACTGGTGCTTACAGCCATAGTCAGGGAATCAAGGAGCTGCGAGATGCCATTGCTGAAGGAATTAAAGCTCGTGATGATTTTCCTGCAGATCCTAATGACATATTTCTAACAAATGGTGCAAGCCCAGCAGTCCATATGGTGATGCAATTGCTGATAAGTACAGAGAAAGATGGAATACTCTGCCCCATTCCTCAGTACCCTCTGTACTCTGCTTCTATAGCTCTTCATGGCGGTACTCTTGTTCCTTACTATCTTGATGAAGCATCGGGATGGGGATTGGAAATTTCTGAGCTGAAGAAGCAATTGGAGTCTGCCAAGTCTAAGGGTATTTCAGTAAGGGCATTGGCGATTATAAATCCCGGCAATCCTACTGGACAAGTTCTCACTAAGGAAAACCAAGAACAAATAGTGAAGTTCTGCAAGCAAGAGGGCCTCGTTCTTCTGGCAGATGAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGATCCCAGACGTCGTATCTCGCCTGACTGACTTCCATAAAGCATTCATGAACGAATATCGCGACTGA

Coding sequence (CDS)

GTTTTGGAATGCGAGTATGCTGTTCGTGGTGAGATTGTCGCCCTCGCTCAGAGACTGGAAAGAGAGCTTCTGACTAATCCAGGCTCACATGCTTTTGATGAGATACTGTACTGCAACATAGGAAATCCTCATTCCCTTGGTCAGCAACCAATAACATTTTTCCGTGAGGTTCTTGCACTGTGTGAGCATCCATCTCTTCTGGAAAGAAACGAAATAAACGGTTTGTTCAGTGAAGATGCCATTAAGCGAGCTTCGCAAATTCTGAAACAAATTCCTGGAAAAGCAACTGGTGCTTACAGCCATAGTCAGGGAATCAAGGAGCTGCGAGATGCCATTGCTGAAGGAATTAAAGCTCGTGATGATTTTCCTGCAGATCCTAATGACATATTTCTAACAAATGGTGCAAGCCCAGCAGTCCATATGGTGATGCAATTGCTGATAAGTACAGAGAAAGATGGAATACTCTGCCCCATTCCTCAGTACCCTCTGTACTCTGCTTCTATAGCTCTTCATGGCGGTACTCTTGTTCCTTACTATCTTGATGAAGCATCGGGATGGGGATTGGAAATTTCTGAGCTGAAGAAGCAATTGGAGTCTGCCAAGTCTAAGGGTATTTCAGTAAGGGCATTGGCGATTATAAATCCCGGCAATCCTACTGGACAAGTTCTCACTAAGGAAAACCAAGAACAAATAGTGAAGTTCTGCAAGCAAGAGGGCCTCGTTCTTCTGGCAGATGAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGGTATATCAAGCAAATATTTATGTTCCAGACAAGAAGTTCCACTCTTTTAAGAAGATTTCACGCAGCATGGGTTATGGTGAAAAGGATATCTCCTTAGTATCATTCCAGTCTGTCTCTAAAGGGTACTATGGTGAGTGTGGAAAACGAGGAGGTTACATGGAGATTACTGGGTTTAGTGCTGATGTGAGGGAGCAAATATACAAAGTGGCATCTGTTAATCTTTGTTCTAATATTTCTGGACAGATTCTTGCTAGCCTTGTGATGAATCCACCAAAGATCCCAGACGTCGTATCTCGCCTGACTGACTTCCATAAAGCATTCATGAACGAATATCGCGACTGA

Protein sequence

VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLALCEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARDDFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYLDEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGLVLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYMEITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYMEITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKIPDVVSRLTDFHKAFMNEYRD
Homology
[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match: F4I7I0 (Alanine aminotransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALAAT1 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 6.1e-167
Identity = 297/469 (63.33%), Postives = 363/469 (77.40%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           V++CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQQPITFFREVLAL
Sbjct: 75  VIKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQQPITFFREVLAL 134

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C + +LL+ +  +GLFS D+I+RA +IL QIPG+ATGAYSHSQGIK LRDAIA+GI+ARD
Sbjct: 135 CSYTALLDESATHGLFSSDSIERAWKILDQIPGRATGAYSHSQGIKGLRDAIADGIEARD 194

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIF+T+GASP VHM+MQLLI++EKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 195 GFPADPNDIFMTDGASPGVHMMMQLLITSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 254

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLE A+SKGI+VRALA+INPGNPTGQVL++ENQ  +VKFCKQEGL
Sbjct: 255 DEASGWGLEISELKKQLEDARSKGITVRALAVINPGNPTGQVLSEENQRDVVKFCKQEGL 314

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ N+YVPDKKFHSFKK++RSMGYGEKD++LVSFQSVSKGYYGECGKRGGYME
Sbjct: 315 VLLADEVYQENVYVPDKKFHSFKKVARSMGYGEKDLALVSFQSVSKGYYGECGKRGGYME 374

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK----VYQANIYVPDKKFHSFKK 360
           +TGF++DVREQIYK+ASVNLCSNISGQILASL+M+PPK     Y++ I   D    S  +
Sbjct: 375 VTGFTSDVREQIYKMASVNLCSNISGQILASLIMSPPKPGDDSYESYIAEKDGILSSLAR 434

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY-------GECGKRGGYMEITGFSADVREQIYKVAS 420
            ++++      +  V+        Y        +        E T       +++ K   
Sbjct: 435 RAKTLEEALNKLEGVTCNRAEGAMYLFPCLHLPQKAIAAAEAEKTAPDNFYCKRLLKATG 494

Query: 421 VNLCSNI-------SGQILASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 452
           + +           +     +++    KIP +V RLT FH++FM+E+RD
Sbjct: 495 IVVVPGSGFRQVPGTWHFRCTILPQEDKIPAIVDRLTAFHQSFMDEFRD 543

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match: Q9LDV4 (Alanine aminotransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALAAT2 PE=1 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 5.7e-165
Identity = 300/469 (63.97%), Postives = 359/469 (76.55%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQ PI FFREVLAL
Sbjct: 72  VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+H SLL+ +E +GLFS D+I RA +IL  IPG+ATGAYSHSQGIK LRD IA GI+ARD
Sbjct: 132 CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIFLT+GASPAVHM+MQLL+S+EKDGIL PIPQYPLYSASIALHGG+LVPYYL
Sbjct: 192 GFPADPNDIFLTDGASPAVHMMMQLLLSSEKDGILSPIPQYPLYSASIALHGGSLVPYYL 251

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEA+GWGLEIS+LKKQLE A+SKGISVRAL +INPGNPTGQVL +ENQ  IV FCKQEGL
Sbjct: 252 DEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEENQRDIVNFCKQEGL 311

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ N+YVPDKKFHSFKK++RS+GYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 312 VLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKGYYGECGKRGGYME 371

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK----VYQANIYVPDKKFHSFKK 360
           +TGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK     Y + +   D    S  K
Sbjct: 372 VTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSYMAERDGILSSMAK 431

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY-------GECGKRGGYMEITGFSADVREQIYKVAS 420
            ++++      +  V+        Y        +        E T   A   +++     
Sbjct: 432 RAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAPDAFYCKRLLNATG 491

Query: 421 VNLCSNIS-GQI------LASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 452
           V +      GQ+        +++    KIP +V+RLT+FHK+FM+E+R+
Sbjct: 492 VVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEFRN 540

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match: P34106 (Alanine aminotransferase 2 OS=Panicum miliaceum OX=4540 PE=1 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 8.5e-161
Identity = 299/469 (63.75%), Postives = 345/469 (73.56%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV  AQ L+++L T PGS  FDEILYCNIGNP SLGQQP+TFFREVLAL
Sbjct: 14  VLKCEYAVRGEIVIHAQHLQQQLQTQPGSLPFDEILYCNIGNPQSLGQQPVTFFREVLAL 73

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+HP LLE+ E   LFS DAI RA QIL  IPG+ATGAYSHSQGIK LRDAIA GI +RD
Sbjct: 74  CDHPCLLEKEETKSLFSADAISRAKQILSTIPGRATGAYSHSQGIKGLRDAIAAGIASRD 133

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPA+ +DIF+T+GASP VHM+MQLLI  EKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 134 GFPANADDIFVTDGASPGVHMMMQLLIRNEKDGILCPIPQYPLYSASIALHGGTLVPYYL 193

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DE +GWGLEIS+LKKQLE A+SKGI VRAL +INPGNPTGQVL ++NQ  IV+FCK EGL
Sbjct: 194 DEKTGWGLEISDLKKQLEDARSKGIDVRALVVINPGNPTGQVLAEDNQCDIVRFCKNEGL 253

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ NIYV DKKF+SFKKI+RS+GYGE D+ LVSFQSVSKGYYGECGKRGGYME
Sbjct: 254 VLLADEVYQENIYVDDKKFNSFKKIARSVGYGEDDLPLVSFQSVSKGYYGECGKRGGYME 313

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSA VREQIYK+ASVNLCSNI+GQILASLVMNPPKV    Y A     D    S  +
Sbjct: 314 ITGFSAPVREQIYKIASVNLCSNITGQILASLVMNPPKVGDESYAAYKAEKDGILQSLAR 373

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY-----------GECGKRGGYMEITGFSADVREQI- 420
            ++++     ++  +S        Y            E  K         ++  + E   
Sbjct: 374 RAKALEDAFNNLEGISCNKAEGAMYLFPQIHLPKKAIEAAKAANKAPDAFYALRLLESTG 433

Query: 421 YKVASVNLCSNISG--QILASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 452
             V   +    + G   I  +++    KIP V++R   FH+AFM EYRD
Sbjct: 434 IVVVPGSGFGQVPGTWHIRCTILPQEDKIPAVITRFKAFHEAFMAEYRD 482

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match: P52894 (Alanine aminotransferase 2 OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.6e-159
Identity = 299/484 (61.78%), Postives = 347/484 (71.69%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV  AQRL+ +L T PGS  FDEILYCNIGNP SLGQQP+TFFREVLAL
Sbjct: 14  VLKCEYAVRGEIVIHAQRLQEQLKTQPGSLPFDEILYCNIGNPQSLGQQPVTFFREVLAL 73

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+HP LL+R EI  LFS D+I RA QIL  IPG+ATGAYSHSQGIK LRDAIA GI +RD
Sbjct: 74  CDHPDLLQREEIKTLFSADSISRAKQILAMIPGRATGAYSHSQGIKGLRDAIASGIASRD 133

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPA+ +DIFLT+GASP VH++MQLLI  EKDGIL PIPQYPLYSASIALHGG LVPYYL
Sbjct: 134 GFPANADDIFLTDGASPGVHLMMQLLIRNEKDGILVPIPQYPLYSASIALHGGALVPYYL 193

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           +E++GWGLE S++KKQLE A+S+GI+VRAL +INPGNPTGQVL +ENQ  IVKFCK EGL
Sbjct: 194 NESTGWGLETSDVKKQLEDARSRGINVRALVVINPGNPTGQVLAEENQYDIVKFCKNEGL 253

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ NIYV +KKFHSFKKI RS+GYGE+D+ LVS+QSVSKGYYGECGKRGGY E
Sbjct: 254 VLLADEVYQENIYVDNKKFHSFKKIVRSLGYGEEDLPLVSYQSVSKGYYGECGKRGGYFE 313

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVYQANIYVPDKKFHSFKKISRS 360
           ITGFSA VREQIYK+ASVNLCSNI+GQILASLVMNPPK         D+ + S+K     
Sbjct: 314 ITGFSAPVREQIYKIASVNLCSNITGQILASLVMNPPK-------ASDESYASYK----- 373

Query: 361 MGYGEKDISLVSFQSVSKGYYG--------ECGKRGGYMEITGFSADVREQIYKVASVNL 420
               EKD  L S    +K             C +  G M +       ++ I    + N 
Sbjct: 374 ---AEKDGILASLARRAKALEHAFNKLEGITCNEAEGAMYVFPQICLPQKAIEAAKAANK 433

Query: 421 CSNI-------------------SGQI------LASLVMNPPKIPDVVSRLTDFHKAFMN 452
             +                     GQ+        +++    KIP V+SR T FH+AFM+
Sbjct: 434 APDAFYALRLLESTGIVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVISRFTVFHEAFMS 482

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match: Q54MJ7 (Probable alanine aminotransferase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=gpt PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.1e-95
Identity = 174/342 (50.88%), Postives = 240/342 (70.18%), Query Frame = 0 

Query: 1   VLECEYAVRGEIV----ALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFRE 60
           V   +YAVRGE+V    A++ +L+++      +  F+EI+YCNIGNP  L Q+P+T+FR+
Sbjct: 63  VRNAQYAVRGELVIRAEAISHQLQKQKTEGTKTLPFEEIVYCNIGNPQQLKQKPLTYFRQ 122

Query: 61  VLALCEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGI 120
           V++L E P LL+   +  ++  D I RA +IL  I    TGAYS+SQGI  +  ++A+ I
Sbjct: 123 VVSLVECPDLLDNPYVEKIYPADVISRAKEILGSI-NNTTGAYSNSQGIGLVLRSVADFI 182

Query: 121 KARDDFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLV 180
           + RD   +DP++IFLT+GAS  V  +++LLI    DGIL PIPQYPLYSA+I L+ G+ +
Sbjct: 183 ERRDGHKSDPSEIFLTDGASVGVQRILKLLIKDRSDGILIPIPQYPLYSATIELYNGSQL 242

Query: 181 PYYLDEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCK 240
            Y L+E  GW LEIS+L+     A SKGI+ RAL IINPGNPTGQ L + N E+IVKFC 
Sbjct: 243 GYLLNEEKGWSLEISQLEHSYNDAVSKGINPRALVIINPGNPTGQCLDRANMEEIVKFCL 302

Query: 241 QEGLVLLADEVYQANIYVPDKK-FHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKR 300
           ++ +VLLADEVYQ N+YV + K F SFKK+ + MG    D+ +VSF SVSKG+ GECGKR
Sbjct: 303 EKNVVLLADEVYQENVYVKESKPFISFKKVVKDMGGDYADLEMVSFHSVSKGFVGECGKR 362

Query: 301 GGYMEITGFSADVREQIYKVASVNLCSNISGQILASLVMNPP 338
           GGYME+ G + DV+ +IYK+AS+ LC N+ GQ++  L++ PP
Sbjct: 363 GGYMELNGVTQDVKAEIYKLASIGLCPNVIGQLVVDLMVRPP 403

[Close]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match: XP_023547733.1 (alanine aminotransferase 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707 bits (1825), Expect = 4.26e-253
Identity = 372/469 (79.32%), Postives = 392/469 (83.58%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLGR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++++      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS------LVMNPPKIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      ++    KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTILPRENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match: KAG6575410.1 (Alanine aminotransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 703 bits (1815), Expect = 1.42e-251
Identity = 369/469 (78.68%), Postives = 392/469 (83.58%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQRLE+ELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRLEKELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQ+QIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQKQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           +LLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 ILLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++++      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTILPPENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match: XP_022953821.1 (alanine aminotransferase 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 700 bits (1807), Expect = 2.34e-250
Identity = 368/469 (78.46%), Postives = 390/469 (83.16%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQRLE+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRLEKELLANPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           +LLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYM 
Sbjct: 260 ILLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYMG 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++++      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTILPPENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match: XP_022992240.1 (alanine aminotransferase 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 698 bits (1801), Expect = 1.14e-249
Identity = 367/469 (78.25%), Postives = 389/469 (82.94%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQR+E+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 6   VLECEYAVRGEIVALAQRMEKELLINPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 65

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 66  CEHPSLLERNEIKGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 125

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVH+VMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 126 DFPADPNDIFLTNGASPAVHLVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 185

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 186 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 245

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 246 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 305

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 306 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 365

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++ +      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 366 RAKRLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEEADTTPDTFYCRQLLNATG 425

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 426 VVVVPGSGFGQVAGTWHFRCTVLPPENKIPDVVSRLTDFHKAFMNEYRD 474

[Close]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match: XP_022992239.1 (alanine aminotransferase 1, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 698 bits (1801), Expect = 1.91e-249
Identity = 367/469 (78.25%), Postives = 389/469 (82.94%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQR+E+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRMEKELLINPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEIKGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVH+VMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHLVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++ +      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKRLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEEADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTVLPPENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match: A0A6J1GP57 (alanine aminotransferase 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111456238 PE=4 SV=1)

HSP 1 Score: 700 bits (1807), Expect = 1.13e-250
Identity = 368/469 (78.46%), Postives = 390/469 (83.16%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQRLE+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRLEKELLANPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           +LLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYM 
Sbjct: 260 ILLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYMG 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++++      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTILPPENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match: A0A6J1JV54 (alanine aminotransferase 1, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488620 PE=4 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 5.54e-250
Identity = 367/469 (78.25%), Postives = 389/469 (82.94%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQR+E+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 6   VLECEYAVRGEIVALAQRMEKELLINPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 65

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 66  CEHPSLLERNEIKGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 125

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVH+VMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 126 DFPADPNDIFLTNGASPAVHLVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 185

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 186 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 245

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 246 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 305

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 306 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 365

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++ +      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 366 RAKRLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEEADTTPDTFYCRQLLNATG 425

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 426 VVVVPGSGFGQVAGTWHFRCTVLPPENKIPDVVSRLTDFHKAFMNEYRD 474

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match: A0A6J1JX08 (alanine aminotransferase 1, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488620 PE=4 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 9.25e-250
Identity = 367/469 (78.25%), Postives = 389/469 (82.94%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VLECEYAVRGEIVALAQR+E+ELL NPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL
Sbjct: 20  VLECEYAVRGEIVALAQRMEKELLINPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           CEHPSLLERNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD
Sbjct: 80  CEHPSLLERNEIKGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
           DFPADPNDIFLTNGASPAVH+VMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 DFPADPNDIFLTNGASPAVHLVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLESAKSKGISVRAL +INPGNPTGQVLTKENQEQIVKFCKQEGL
Sbjct: 200 DEASGWGLEISELKKQLESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVKFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  +
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAR 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY--------GECGKRGGYMEITGFSADVREQIYKVA 420
            ++ +      +  V+        Y         +  K     + T  +   R+ +    
Sbjct: 380 RAKRLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEEADTTPDTFYCRQLLNATG 439

Query: 421 SVNLCSNISGQILAS----LVMNPP--KIPDVVSRLTDFHKAFMNEYRD 451
            V +  +  GQ+  +      + PP  KIPDVVSRLTDFHKAFMNEYRD
Sbjct: 440 VVVVPGSGFGQVAGTWHFRCTVLPPENKIPDVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match: A0A6J1HLX5 (alanine aminotransferase 2, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111464176 PE=4 SV=1)

HSP 1 Score: 660 bits (1703), Expect = 7.49e-235
Identity = 349/480 (72.71%), Postives = 385/480 (80.21%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV LAQ+L+ ELL+ PGSH FDEILYCNIGNP SLGQQPITFFREVLAL
Sbjct: 20  VLKCEYAVRGEIVVLAQKLQEELLSKPGSHPFDEILYCNIGNPQSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+HPSLL+RNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIK LRDAIA+GIKARD
Sbjct: 80  CDHPSLLDRNEIQGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKGLRDAIADGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIFLT+GASPAVHM+MQLLIS+EKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 GFPADPNDIFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEIS+LKKQLESA+SK ISVRAL +INPGNPTGQVLTKENQEQIV+FCKQEGL
Sbjct: 200 DEASGWGLEISDLKKQLESARSKNISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ NIYVPDKKFHSFKKISR+MGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 VLLADEVYQENIYVPDKKFHSFKKISRTMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S   
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHVYESYCAERDGILSSLAT 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYM----------------EITGFSADV 420
            ++++     + +  S ++V+      C K  G M                E    + D 
Sbjct: 380 RAKTL-----EAAFNSLENVT------CNKAEGAMYLFPRIELPEKAIKAAEAANTAPDA 439

Query: 421 ---REQIYKVASVNLCSNISGQI------LASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 451
              R+ ++    V +  +  GQ+        +++    KIP VVSRLTDFHKAFMNEYRD
Sbjct: 440 FYCRQLLHATGIVVVPGSGFGQVPGTWHFRCTILPQEGKIPAVVSRLTDFHKAFMNEYRD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match: A0A6J1KF09 (alanine aminotransferase 2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111494419 PE=4 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 1.51e-234
Identity = 349/480 (72.71%), Postives = 385/480 (80.21%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV LAQ+L+ ELL+ PGSH FDEILYCNIGNP SLGQQPITFFREVLAL
Sbjct: 20  VLKCEYAVRGEIVVLAQKLQEELLSKPGSHPFDEILYCNIGNPQSLGQQPITFFREVLAL 79

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+HPSLL+RNEI GLFSEDAIKRASQILKQIPGKATGAYSHSQGIK LRDAIA+GIKARD
Sbjct: 80  CDHPSLLDRNEIQGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKGLRDAIADGIKARD 139

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIFLT+GASPAVHM+MQLLIS+EKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 140 GFPADPNDIFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 199

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEIS+LKKQLESA+SK ISVRAL +INPGNPTGQVLTKENQEQIV+FCKQEGL
Sbjct: 200 DEASGWGLEISDLKKQLESARSKNISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 259

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ NIYVPDKKFHSFKKISR+MGYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 260 VLLADEVYQENIYVPDKKFHSFKKISRTMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 319

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV----YQANIYVPDKKFHSFKK 360
           ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKV    Y++     D    S  K
Sbjct: 320 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDHIYESYCAERDGILSSLAK 379

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYM----------------EITGFSADV 420
            ++++     + +  S ++V+      C K  G M                E    + D 
Sbjct: 380 RAKTL-----EAAFNSLENVT------CNKAEGAMYLFPRIELPEKAIKAAEAANTAPDA 439

Query: 421 ---REQIYKVASVNLCSNISGQI------LASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 451
              R+ ++    V +  +  GQ+        +++    KIP VVSRLTDFHKAFMNEY D
Sbjct: 440 FYCRQLLHATGIVVVPGSGFGQVPGTWHFRCTILPQERKIPAVVSRLTDFHKAFMNEYCD 488

[Close]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match: AT1G17290.1 (alanine aminotransferas )

HSP 1 Score: 588.6 bits (1516), Expect = 4.3e-168
Identity = 297/469 (63.33%), Postives = 363/469 (77.40%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           V++CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQQPITFFREVLAL
Sbjct: 75  VIKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQQPITFFREVLAL 134

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C + +LL+ +  +GLFS D+I+RA +IL QIPG+ATGAYSHSQGIK LRDAIA+GI+ARD
Sbjct: 135 CSYTALLDESATHGLFSSDSIERAWKILDQIPGRATGAYSHSQGIKGLRDAIADGIEARD 194

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIF+T+GASP VHM+MQLLI++EKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 195 GFPADPNDIFMTDGASPGVHMMMQLLITSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 254

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEASGWGLEISELKKQLE A+SKGI+VRALA+INPGNPTGQVL++ENQ  +VKFCKQEGL
Sbjct: 255 DEASGWGLEISELKKQLEDARSKGITVRALAVINPGNPTGQVLSEENQRDVVKFCKQEGL 314

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ N+YVPDKKFHSFKK++RSMGYGEKD++LVSFQSVSKGYYGECGKRGGYME
Sbjct: 315 VLLADEVYQENVYVPDKKFHSFKKVARSMGYGEKDLALVSFQSVSKGYYGECGKRGGYME 374

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK----VYQANIYVPDKKFHSFKK 360
           +TGF++DVREQIYK+ASVNLCSNISGQILASL+M+PPK     Y++ I   D    S  +
Sbjct: 375 VTGFTSDVREQIYKMASVNLCSNISGQILASLIMSPPKPGDDSYESYIAEKDGILSSLAR 434

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY-------GECGKRGGYMEITGFSADVREQIYKVAS 420
            ++++      +  V+        Y        +        E T       +++ K   
Sbjct: 435 RAKTLEEALNKLEGVTCNRAEGAMYLFPCLHLPQKAIAAAEAEKTAPDNFYCKRLLKATG 494

Query: 421 VNLCSNI-------SGQILASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 452
           + +           +     +++    KIP +V RLT FH++FM+E+RD
Sbjct: 495 IVVVPGSGFRQVPGTWHFRCTILPQEDKIPAIVDRLTAFHQSFMDEFRD 543

[Close]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match: AT1G72330.1 (alanine aminotransferase 2 )

HSP 1 Score: 582.0 bits (1499), Expect = 4.1e-166
Identity = 300/469 (63.97%), Postives = 359/469 (76.55%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQ PI FFREVLAL
Sbjct: 72  VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+H SLL+ +E +GLFS D+I RA +IL  IPG+ATGAYSHSQGIK LRD IA GI+ARD
Sbjct: 132 CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIFLT+GASPAVHM+MQLL+S+EKDGIL PIPQYPLYSASIALHGG+LVPYYL
Sbjct: 192 GFPADPNDIFLTDGASPAVHMMMQLLLSSEKDGILSPIPQYPLYSASIALHGGSLVPYYL 251

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEA+GWGLEIS+LKKQLE A+SKGISVRAL +INPGNPTGQVL +ENQ  IV FCKQEGL
Sbjct: 252 DEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEENQRDIVNFCKQEGL 311

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ N+YVPDKKFHSFKK++RS+GYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 312 VLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKGYYGECGKRGGYME 371

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK----VYQANIYVPDKKFHSFKK 360
           +TGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK     Y + +   D    S  K
Sbjct: 372 VTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSYMAERDGILSSMAK 431

Query: 361 ISRSMGYGEKDISLVSFQSVSKGYY-------GECGKRGGYMEITGFSADVREQIYKVAS 420
            ++++      +  V+        Y        +        E T   A   +++     
Sbjct: 432 RAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAPDAFYCKRLLNATG 491

Query: 421 VNLCSNIS-GQI------LASLVMNPPKIPDVVSRLTDFHKAFMNEYRD 452
           V +      GQ+        +++    KIP +V+RLT+FHK+FM+E+R+
Sbjct: 492 VVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEFRN 540

[Close]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match: AT1G72330.3 (alanine aminotransferase 2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.5e-163
Identity = 300/482 (62.24%), Postives = 359/482 (74.48%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQ PI FFREVLAL
Sbjct: 72  VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+H SLL+ +E +GLFS D+I RA +IL  IPG+ATGAYSHSQGIK LRD IA GI+ARD
Sbjct: 132 CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 121 DFPADPNDIFLTNGASPA-------------VHMVMQLLISTEKDGILCPIPQYPLYSAS 180
            FPADPNDIFLT+GASPA             VHM+MQLL+S+EKDGIL PIPQYPLYSAS
Sbjct: 192 GFPADPNDIFLTDGASPAVTIKTKTLSHRVRVHMMMQLLLSSEKDGILSPIPQYPLYSAS 251

Query: 181 IALHGGTLVPYYLDEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKEN 240
           IALHGG+LVPYYLDEA+GWGLEIS+LKKQLE A+SKGISVRAL +INPGNPTGQVL +EN
Sbjct: 252 IALHGGSLVPYYLDEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEEN 311

Query: 241 QEQIVKFCKQEGLVLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKG 300
           Q  IV FCKQEGLVLLADEVYQ N+YVPDKKFHSFKK++RS+GYGEKDISLVSFQSVSKG
Sbjct: 312 QRDIVNFCKQEGLVLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKG 371

Query: 301 YYGECGKRGGYMEITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK----VYQAN 360
           YYGECGKRGGYME+TGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK     Y + 
Sbjct: 372 YYGECGKRGGYMEVTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSY 431

Query: 361 IYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYY-------GECGKRGGYMEITGF 420
           +   D    S  K ++++      +  V+        Y        +        E T  
Sbjct: 432 MAERDGILSSMAKRAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAP 491

Query: 421 SADVREQIYKVASVNLCSNIS-GQI------LASLVMNPPKIPDVVSRLTDFHKAFMNEY 452
            A   +++     V +      GQ+        +++    KIP +V+RLT+FHK+FM+E+
Sbjct: 492 DAFYCKRLLNATGVVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEF 551

[Close]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match: AT1G72330.2 (alanine aminotransferase 2 )

HSP 1 Score: 569.3 bits (1466), Expect = 2.7e-162
Identity = 274/338 (81.07%), Postives = 309/338 (91.42%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           VL+CEYAVRGEIV +AQ+L+ +L TN  ++ FDEI+YCNIGNP SLGQ PI FFREVLAL
Sbjct: 72  VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+H SLL+ +E +GLFS D+I RA +IL  IPG+ATGAYSHSQGIK LRD IA GI+ARD
Sbjct: 132 CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            FPADPNDIFLT+GASPAVHM+MQLL+S+EKDGIL PIPQYPLYSASIALHGG+LVPYYL
Sbjct: 192 GFPADPNDIFLTDGASPAVHMMMQLLLSSEKDGILSPIPQYPLYSASIALHGGSLVPYYL 251

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DEA+GWGLEIS+LKKQLE A+SKGISVRAL +INPGNPTGQVL +ENQ  IV FCKQEGL
Sbjct: 252 DEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEENQRDIVNFCKQEGL 311

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYGEKDISLVSFQSVSKGYYGECGKRGGYME 300
           VLLADEVYQ N+YVPDKKFHSFKK++RS+GYGEKDISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 312 VLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKGYYGECGKRGGYME 371

Query: 301 ITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK 339
           +TGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK
Sbjct: 372 VTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPK 409

[Close]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match: AT1G23310.1 (glutamate:glyoxylate aminotransferase )

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-94
Identity = 174/339 (51.33%), Postives = 234/339 (69.03%), Query Frame = 0 

Query: 1   VLECEYAVRGEIVALAQRLERELLTNPGSHAFDEILYCNIGNPHSLGQQPITFFREVLAL 60
           V +C+YAVRGE+   A  L++E           +I++ N+GNPH+LGQ+P+TF R+V+AL
Sbjct: 15  VKKCQYAVRGELYLRASELQKE---------GKKIIFTNVGNPHALGQKPLTFPRQVVAL 74

Query: 61  CEHPSLLERNEINGLFSEDAIKRASQILKQIPGKATGAYSHSQGIKELRDAIAEGIKARD 120
           C+ P LL+   +  LF  DAI RA   L    G   GAYS S+G+  +R  +AE I+ RD
Sbjct: 75  CQAPFLLDDPNVGMLFPADAIARAKHYLSLTSG-GLGAYSDSRGLPGVRKEVAEFIQRRD 134

Query: 121 DFPADPNDIFLTNGASPAVHMVMQLLISTEKDGILCPIPQYPLYSASIALHGGTLVPYYL 180
            +P+DP  IFLT+GAS  V  ++  +I    DGIL P+PQYPLYSA+I+L GGTLVPYYL
Sbjct: 135 GYPSDPELIFLTDGASKGVMQILNCVIRGNGDGILVPVPQYPLYSATISLLGGTLVPYYL 194

Query: 181 DEASGWGLEISELKKQLESAKSKGISVRALAIINPGNPTGQVLTKENQEQIVKFCKQEGL 240
           DE+  WGL+++ L++ +  A+S+GI+VRA+ IINPGNPTGQ L++ N  +I+KFC  E L
Sbjct: 195 DESENWGLDVANLRQSVAQARSQGITVRAMVIINPGNPTGQCLSEANIREILKFCYNEKL 254

Query: 241 VLLADEVYQANIYVPDKKFHSFKKISRSMGYG-EKDISLVSFQSVSKGYYGECGKRGGYM 300
           VLL DEVYQ NIY  ++ F S KK+   MG    K++ LVSF +VSKGY+GECG+RGGY 
Sbjct: 255 VLLGDEVYQQNIYQDERPFISSKKVLMEMGSPFSKEVQLVSFHTVSKGYWGECGQRGGYF 314

Query: 301 EITGFSADVREQIYKVASVNLCSNISGQILASLVMNPPK 339
           E+T     V E+IYKVAS+ L  N+S QI   L++NPPK
Sbjct: 315 EMTNLPPRVVEEIYKVASIALSPNVSAQIFMGLMVNPPK 343

The following BLAST results are available for this feature:
BLAST of Cp4.1LG12g09530 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4I7I06.1e-16763.33Alanine aminotransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALA... [more]
Q9LDV45.7e-16563.97Alanine aminotransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALA... [more]
P341068.5e-16163.75Alanine aminotransferase 2 OS=Panicum miliaceum OX=4540 PE=1 SV=1[more]
P528943.6e-15961.78Alanine aminotransferase 2 OS=Hordeum vulgare OX=4513 PE=1 SV=1[more]
Q54MJ72.1e-9550.88Probable alanine aminotransferase, mitochondrial OS=Dictyostelium discoideum OX=... [more]
BLAST of Cp4.1LG12g09530 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023547733.14.26e-25379.32alanine aminotransferase 1, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
KAG6575410.11.42e-25178.68Alanine aminotransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp... [more]
XP_022953821.12.34e-25078.46alanine aminotransferase 1, mitochondrial-like [Cucurbita moschata][more]
XP_022992240.11.14e-24978.25alanine aminotransferase 1, mitochondrial-like isoform X2 [Cucurbita maxima][more]
XP_022992239.11.91e-24978.25alanine aminotransferase 1, mitochondrial-like isoform X1 [Cucurbita maxima][more]
BLAST of Cp4.1LG12g09530 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GP571.13e-25078.46alanine aminotransferase 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JV545.54e-25078.25alanine aminotransferase 1, mitochondrial-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1JX089.25e-25078.25alanine aminotransferase 1, mitochondrial-like isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1HLX57.49e-23572.71alanine aminotransferase 2, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KF091.51e-23472.71alanine aminotransferase 2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
BLAST of Cp4.1LG12g09530 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G17290.14.3e-16863.33alanine aminotransferas [more]
AT1G72330.14.1e-16663.97alanine aminotransferase 2 [more]
AT1G72330.32.5e-16362.24alanine aminotransferase 2 [more]
AT1G72330.22.7e-16281.07alanine aminotransferase 2 [more]
AT1G23310.11.1e-9451.33glutamate:glyoxylate aminotransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 1..45
e-value: 7.0E-154
score: 514.8
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 339..430
e-value: 1.6E-40
score: 141.1
coord: 97..338
e-value: 7.0E-154
score: 514.8
NoneNo IPR availableGENE3D1.10.287.1970coord: 46..96
e-value: 7.0E-154
score: 514.8
NoneNo IPR availablePANTHERPTHR11751:SF473ALANINE AMINOTRANSFERASE 2coord: 339..433
coord: 1..339
NoneNo IPR availableCDDcd00609AAT_likecoord: 99..340
e-value: 5.51654E-46
score: 160.97
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 76..339
e-value: 9.0E-33
score: 113.9
IPR045088Aminotransferase ALAT1/2-likePANTHERPTHR11751ALANINE AMINOTRANSFERASEcoord: 339..433
coord: 1..339
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 343..447
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 78..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g09530.1Cp4.1LG12g09530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009058 biosynthetic process
molecular_function GO:0004021 L-alanine:2-oxoglutarate aminotransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0008483 transaminase activity