Cp4.1LG12g07740 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g07740
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cp4.1LG12: 6752104 .. 6756949 (-)
RNA-Seq Expression	Cp4.1LG12g07740
Synteny	Cp4.1LG12g07740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCAGAAACGGATCCAAGGATGGATGCGGGGCTGCCGGTGATTAACTTCAGCGGCGACGATGTGAAGGCGGAGGGCGGCGAGGGGTGGAGGAGGAGGAGCGGAGAAGTCCGAGAAGCCCTGGAAGAGTACGGGTGTTTTATCGCAGAGTACGAGGGGATTAGCCCTGAGCTACGCGTCGAGGTTTTCAAGGCCGTGGAAGCACTTTTTGATCTTCCAATTGAAACCAAGAAGAAGAACAGATACGAAAAGCCCTTTAATGGGTATGTCGGGGAGCTTCGTACTCTCCCTCTGCATGAAAGCTTGGGCATTGACGACGCCACAAACATCCATGGCGTTCAGGCCTTCACTCACCTCATGTGGCCCAATGGAAACCACCATTTTAGGTGATTTTTTGAATGCTTTCCTAAGTTTATACCATAATTTTGATTTCATTTTGTAACCGCCCAAACACGCCCACCACTATCTCGGTCTTCTTTTGAGTTTTCACTCTCGAGCTTTCTCTCACGGTTTTTAAAATGTGTCTACTAGGTAGAGGTTTACCTACCCTTTTAGAGAATATTTCTTTCCACCAACGTGAGATCTCACAGAGTGTCCTGTCACTAGTTCATTTCTCCAATTATCGTGGGATCCCCAAATCTACCTTTTGTGGGCCCAGTGTCTTTGCTGGCATATTGTCTCTCCACCCTTTTCTGGGCTTAGCTTCCTCGCTAGTACATCACCTGATTTCTGGCTCTGATGCAACAACAGTTGTGAAGGAGAACTAACCATTCTTTAAAAGGGTGGGAAAACGTCTCCATACTAGACACTTTTTAAAAACTTTGAGGAAAAGTCCGAAATGGAAAATATAAAAATAACAATATCTGCTAGTGGTGAGCTTGGACAGTTAATGATGTTCTTGTTTGGTTTGTTTAGTTCGAATTTGCATTCGTTTGCTAAGAAAGCTGAAGAATTGGATCAAATGGTGACGAAAATGATATTTGAAAGCTATGGAGTTGAGAAGTATCATGAGACTCATGCTGCTTCCATTACTTATCTTCTTAGAGCTTTGAAGAACAAAACTCCTCACTCTCAAAACCCTACTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATACCAATGGCTTGGAGGTCCAGACTAAAGATGGCCGGTGGATTCAGGTCGATAACTCCTCACCCTCGACCTTCGTCGTCATTGCCGGGGATGCATTCAAGGTCGAGTATACATATATCTATATACTCTCACTAAATCCTTACTAAAAAAAATGAACATAACCTAACCTAACCCAACTCGATCATAAAATTTTTAGATTGAATTGGTTGAACTGGTTCGTGGCTAGCTCATAAAACATTTATTTATTTATTTTCAAATATTAAATTAAAATTTTCAACTCAATTTTATTTTATATTTTGAAAATTTATTTTCGTTAAATTATTTTTAAGAGTGGGTAGAGAATAGATAATCATGAAATTAAATAAAAATAAAATAACTAGTAATAAATTCAGGTCGGTTTGGTTCAACTTGACATAGGAGAACCCATGAACTAATCAAAAGCATAGTTTTTTTTTTTAATTTGAACCCAGTCCATGGGTTAGGTTGATCGAGTTTTTCGGGTCATCTAATTTTTTAAACATAAAATATTTGGACGAAAACGAAAGGGTATCTAAGTTACAGAGACCAAAATGATATTTTAACATAAAGTATACTATTCGAAGATCAATTACTTCTCAAAACCATCACGTTTTGTTCTTGAACTTTTAAAAAACGTTAAGGACTAAACGTTCGAGTATAAACCCTAATTACGGTGTTGGATTTTGGTAGAGAAGGCTTGGAGCAATGACAGAATAGAGTCACCAACTCACAAAGTGACAATAAGAGGGAACGAAGATAGGTACTCTCTTGCAATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCCAAAGAGCTCATAGACCAAACCCATCCTTTGAGATATAAACCCTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCAAAAGAGGGTTTCAAATCCAAATCTCCACTCCAAGACTACTGTGCTCTCTAAATCAACGAACACCTATCATGTTTGTGTATGATTATAGTTCTAAGTTTCAATGTGTTCATGAGCTTTTGCTTTGTTTGTGTGCTTTTAGGTTAAGAAAGAATGTATGAACTCTAATAAACTTCGTGTGTTCGCTCTTCTTAATATATAAACATAGTTTCCGATTCGTAGTTTCAAATTCAAATAATCGTCCCGATTGATATCAGTTATGTTTGTTTTCTCACGACCTTTTTATCATTTTTTTATTTTATTTTTAATCAAACATCTGAATTTCTAAGATAAATTCAAAAAAACTAAAGAAATCTATTAGTGAAAATAGTGTTTATAAGCTTACTTTTTAACAGAGGTGTTAAAAAAGAACCAGATGATCAGAAGAACCTAACCAACTCAACTTGACCATATAGTTTTGGTTGGGTTGGGTTCATATAAATAAAAATTTTATGCATTGGATTGATTCATAATCGAATTAATTCTAATTTATTGTTATTTTTAAAATATACATTTTATATATATATATTATGAATTAAATTTTTAAAAAATTTATAGCTAGGTTGTGGTAAAAAAAAATTGCATTCAAACAATTAGATTGAATCTAAAAAAATGTCTCAGTCTGATTTAAATTAATCCAGGAACATCGGTACTTTACAAAAGTCAAAAACTCATACGTCAGCTATGTCAGATATTCGAAAATTAAATTTGTGTAATTTTACCAACTTTATCAAGAACGCTTCGAATTCATATAACATTAAACAATATTAGTACAACATTGAAACAAAAATATAATAAAGTATTGTTTAGTTGAAATGTATGAATCAAATATTAATCTGACGTATATTTCCATATCTATTTCGTATTCTTTTAATTCAATCATTTAGAGAGAAATGATTTAATTAAACCAATTATGATGACTTAAAATATATATATATGAAATAACCCCACAAATTAAATTTAATCTCAAATCCACATCGGTGACCACAAACAATTGATTATACAAACACATGAATCATCGCAAAAAAAAAAAAAAAAAAAAAGCNTCTTACATAAGCTATCAAACCAAGGAAGAACAGGTAGCTGTGGGTTAGGCATTGGTGACAATGGGGTCTGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGATTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGATGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGGATCAAGGAAACGATCGCTTCAGGTCACTTCTGAACTCGAATTCAACGTCTTAACAACTATGTAACTATGTTCATGTTTGTGAAAGTTATTTAAGAAAATTGACTCACGGGAGAAAAAGTTAAGTTATAAGTTTAGTTTATGTGTTTATTTAGTTCATAAACTTTAAAAATGTTTTGATTCGTCCTAAACTTTTAATTTTCTGTTTAATAGGTTTGTAAATTTTTAGAAATTTTGATTAGGGGAGACATGGTGTCAACTCTTTACCACTATGTGGTCCAAGGGAGCTATCTTATGTCACGTGAGTGTAATGGTTGGACGAGCGTTGTAAGACGAATGTTTTGGGTGTCTTCCTTTGCAAATGGGTTTTCGAAAATAATGTCGTAGACATGGGTGAGTTGACATTGGGTCATGTCAAACTTCTTAAACATTTAAACTTTAGGATCAAAACGACACAAACTTGAAAGTTCATAAATTGTGAAATCTCACGTCAGTTGGAGATGAGAACAAAGTATTTTTTATAAGGGTATGGAAACCTTTCCTAGTAAGACACGTTTTAAAACTGCGAGACTGATAACGATATGTAATGGGCCAAAGTAGACAATATCTACTGGCGGTGGGCTCTGACTAAATGTATCAGAGCGAGATGGATGGTGTGTCAGCGAGGACTCTAGTCTCAAAGGGGGTGAATTGTGAGATCCTACGTCATTGTAGAGGAGAACAAAATATTCCTTATAAGAGTGTGGAAACCTCTACCTAGAAGATGCATTTTAAAACCGTGAGGCTAACGGGTTAAAGTAGACAATATTACTAACAGTGGACTTGTGACTTAGACTATTACATAAATTGAACTTATAATTTAACCTAACTTAGTCAGAACGTTGCTTACCATTCAAATAATGGAACGTTGAAAGTTAGATCTTCGAGTCATCTTTTATAAAAATTGGAAAAAAAAAAAGGGAGTTTTTCATTTTTATTATCTTCCCTTGACGAAATCACAGCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACACCTCATCGGTGGTCTCCAAATTCAATCGCTCGACGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTC

mRNA sequence

ATGGAAACGGATCCAAGGATGGATGCGGGGCTGCCGGTGATTAACTTCAGCGGCGACGATGTGAAGGCGGAGGGCGGCGAGGGGTGGAGGAGGAGGAGCGGAGAAGTCCGAGAAGCCCTGGAAGAGTACGGGTGTTTTATCGCAGAGTACGAGGGGATTAGCCCTGAGCTACGCGTCGAGGTTTTCAAGGCCGTGGAAGCACTTTTTGATCTTCCAATTGAAACCAAGAAGAAGAACAGATACGAAAAGCCCTTTAATGGGTATGTCGGGGAGCTTCGTACTCTCCCTCTGCATGAAAGCTTGGGCATTGACGACGCCACAAACATCCATGGCGTTCAGGCCTTCACTCACCTCATCTATGGAGTTGAGAAGTATCATGAGACTCATGCTGCTTCCATTACTTATCTTCTTAGAGCTTTGAAGAACAAAACTCCTCACTCTCAAAACCCTACTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATACCAATGGCTTGGAGGTCCAGACTAAAGATGGCCGGTGGATTCAGGTCGATAACTCCTCACCCTCGACCTTCGTCGTCATTGCCGGGGATGCATTCAAGGTCGAAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGATTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGATGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGGATCAAGGAAACGATCGCTTCAGGTCACTTCTGAACTCGAATTCAACCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACACCTCATCGGTGGTCTCCAAATTCAATCGCTCGACGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTC

Coding sequence (CDS)

ATGGAAACGGATCCAAGGATGGATGCGGGGCTGCCGGTGATTAACTTCAGCGGCGACGATGTGAAGGCGGAGGGCGGCGAGGGGTGGAGGAGGAGGAGCGGAGAAGTCCGAGAAGCCCTGGAAGAGTACGGGTGTTTTATCGCAGAGTACGAGGGGATTAGCCCTGAGCTACGCGTCGAGGTTTTCAAGGCCGTGGAAGCACTTTTTGATCTTCCAATTGAAACCAAGAAGAAGAACAGATACGAAAAGCCCTTTAATGGGTATGTCGGGGAGCTTCGTACTCTCCCTCTGCATGAAAGCTTGGGCATTGACGACGCCACAAACATCCATGGCGTTCAGGCCTTCACTCACCTCATCTATGGAGTTGAGAAGTATCATGAGACTCATGCTGCTTCCATTACTTATCTTCTTAGAGCTTTGAAGAACAAAACTCCTCACTCTCAAAACCCTACTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATACCAATGGCTTGGAGGTCCAGACTAAAGATGGCCGGTGGATTCAGGTCGATAACTCCTCACCCTCGACCTTCGTCGTCATTGCCGGGGATGCATTCAAGGTCGAAGTGCCCGTAATAGACTTGTCCAAATTAGAGAAGTTGGAGGCTGGCAGTGACGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTGTTTGAGGAGGTTTCTTTGGAGATTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGCCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGATAACCCAACTCAGCTAGATGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCGGATCAAGGAAACGATCGCTTCAGGTCACTTCTGAACTCGAATTCAACCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAGTAACAAGAATGGTGCTGGAAAGCTACGGAGTATCAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACAGAGTTCCTGAGCAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACACCTCATCGGTGGTCTCCAAATTCAATCGCTCGACGATCAATGGATCGACCTCGAGCCCTCCAGCCACTGCTCCTTC

Protein sequence

METDPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEVFKAVEALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLIYGVEKYHETHAASITYLLRALKNKTPHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF

Homology

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BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 9.4e-40
Identity = 85/227 (37.44%), Postives = 136/227 (59.91%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+E++ S F A+++LF
Sbjct: 6   QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 265 QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFR 324
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WPD G     
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHG----- 125

Query: 325 SLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP- 384
              N + +ETI  FS  + +LD +V RM++ES+G+ K  ID +++ST  L R  KY  P 
Sbjct: 126 ---NKSISETIHLFSEQLVELDLMVRRMIMESFGIEK-YIDEHLNSTYYLTRLMKYTSPP 185

Query: 385 ---EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS 428
              +  ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS
Sbjct: 186 DDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS 218

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.2e-39
Identity = 85/228 (37.28%), Postives = 136/228 (59.65%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+E++ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 265 QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFR 324
           +LPI T+ +N S K +HGY        LYES+ ID+   L+    F   +WPD G     
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHG----- 132

Query: 325 SLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP- 384
              N + +ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P 
Sbjct: 133 ---NKSISETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPP 192

Query: 385 ----EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS 428
               +  ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS
Sbjct: 193 DDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS 226

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.7e-39
Identity = 84/229 (36.68%), Postives = 136/229 (59.39%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+E++ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 265 QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFR 324
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WPD G     
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHG----- 132

Query: 325 SLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP- 384
              N + +ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P 
Sbjct: 133 ---NKSISETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPP 192

Query: 385 -----EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS 428
                +  ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS
Sbjct: 193 DDDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS 227

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.3e-29
Identity = 76/224 (33.93%), Postives = 120/224 (53.57%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G    + +PVIDLS ++ L+ G+ +W S    +R  LEE+GCF  V + VS E+  +   
Sbjct: 2   GSTAPLRLPVIDLS-MKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCN 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
              EL  LP+ET++ N ++  Y G+    S   L E M +   T L+  + F NLMWP +
Sbjct: 62  KGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-E 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GN  F         ET+  +   MA++D I+  MV ES+G+ K   D+  +ST  LL F 
Sbjct: 122 GNKDF--------CETVYSYGKRMAEVDHILKMMVFESFGMEKH-FDSFCESTNYLLHFM 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWI 423
           +Y+ P +      L  H D +  +I++Q+ + GL+ ++ D +WI
Sbjct: 182 RYQQPGKDGRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWI 211

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 3.5e-26
Identity = 67/195 (34.36%), Postives = 107/195 (54.87%), Query Frame = 0


Query: 204 VEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDEL 263
           +++P+I+L+  + LE GS  W      +R  LE+FGCF   +++VSLE+  S    ++EL
Sbjct: 6   LQLPLINLAD-KTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 65

Query: 264 FQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRF 323
           F LP+ET+ +N   K Y GY    +   L ES+ I N   L+    F   +WPD      
Sbjct: 66  FALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDG--- 125

Query: 324 RSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP 383
               N N ++TIQ F+  + ++D +V RMV+ES+G+ K  ID ++ ST   +R  KY  P
Sbjct: 126 ----NENISKTIQLFAEKLVEIDVMVRRMVMESFGIEK-YIDDHLKSTEYRMRLMKYIAP 185

Query: 384 EQHETDVGLHAHTDL 399
            + + +  +  + DL
Sbjct: 186 PEGDANTTVDDYADL 188

[Close]

BLAST of Cp4.1LG12g07740 vs. NCBI nr
Match: KAG7014111.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 712 bits (1837), Expect = 4.14e-254
Identity = 373/483 (77.23%), Postives = 378/483 (78.26%), Query Frame = 0


Query: 2   ETDPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV 61
           ET PRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV
Sbjct: 32  ETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV 91

Query: 62  FKAVEALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLIYG 121
           FKAVEALF+LPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNI            
Sbjct: 92  FKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNI-----------D 151

Query: 122 VEKYHETHAASITYLLRALKNKTPHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDG 181
           VEKYHETHAASITYLLRALKNK PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDG
Sbjct: 152 VEKYHETHAASITYLLRALKNKAPHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDG 211

Query: 182 RWIQVDNSSPSTFVVIAGDAFK--------------------------VEVPVI------ 241
           RWIQVDNSSPSTFV  + D  +                          VEVP        
Sbjct: 212 RWIQVDNSSPSTFVAWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTH 271

Query: 242 --------------------DLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVS 301
                               DLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVS
Sbjct: 272 PLRYKPFDHIGLLHFFRSKEDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVS 331

Query: 302 LEIHNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQS 361
           LE+HNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQS
Sbjct: 332 LELHNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQS 391

Query: 362 FANLMWPDQGNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNID 421
           FANLMWPDQGNDRF                                SYGVSKQLIDTN+D
Sbjct: 392 FANLMWPDQGNDRF--------------------------------SYGVSKQLIDTNVD 451

Query: 422 STANLLRFFKYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSH 432
           STANLLRFFKYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSH
Sbjct: 452 STANLLRFFKYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSH 471

[Close]

BLAST of Cp4.1LG12g07740 vs. NCBI nr
Match: XP_023547382.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439 bits (1128), Expect = 3.77e-150
Identity = 219/234 (93.59%), Postives = 221/234 (94.44%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG
Sbjct: 2   GSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GNDRF        +ETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF
Sbjct: 122 GNDRF--------SETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF
Sbjct: 182 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. NCBI nr
Match: KAG6575570.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434 bits (1116), Expect = 2.50e-148
Identity = 215/234 (91.88%), Postives = 221/234 (94.44%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFFG
Sbjct: 2   GSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP+Q
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPNQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GNDRF        +ETIQRFSTL+AKLDKIVTRMVLESYGVSKQLIDTN+DSTANLLRFF
Sbjct: 122 GNDRF--------SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNVDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF
Sbjct: 182 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 432 bits (1112), Expect = 1.01e-147
Identity = 215/234 (91.88%), Postives = 221/234 (94.44%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLEKLEAG+DAWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFFG
Sbjct: 2   GSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GN+RF        +ETIQRFSTL+AKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF
Sbjct: 122 GNNRF--------SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF
Sbjct: 182 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 420 bits (1080), Expect = 7.26e-143
Identity = 207/234 (88.46%), Postives = 217/234 (92.74%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLE LEAG++AWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFFG
Sbjct: 2   GSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAI+NPTQLDAAQSFANLMWP Q
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GN+ F        +ETIQRFSTLMAKLDKI+TRMVLESYGV+KQLIDTNIDSTANLLRFF
Sbjct: 122 GNNHF--------SETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPE+HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS HCSF
Sbjct: 182 KYRVPEEHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 432 bits (1112), Expect = 4.90e-148
Identity = 215/234 (91.88%), Postives = 221/234 (94.44%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLEKLEAG+DAWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFFG
Sbjct: 2   GSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GN+RF        +ETIQRFSTL+AKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF
Sbjct: 122 GNNRF--------SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF
Sbjct: 182 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 3.51e-143
Identity = 207/234 (88.46%), Postives = 217/234 (92.74%), Query Frame = 0


Query: 199 GDAFKVEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFG 258
           G   +  VPVIDLSKLE LEAG++AWGSTCKAMRVGLEEFGCFCVVFEEVSLE+HNSFFG
Sbjct: 2   GSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFG 61

Query: 259 AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQ 318
           AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAI+NPTQLDAAQSFANLMWP Q
Sbjct: 62  AVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQ 121

Query: 319 GNDRFRSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFF 378
           GN+ F        +ETIQRFSTLMAKLDKI+TRMVLESYGV+KQLIDTNIDSTANLLRFF
Sbjct: 122 GNNHF--------SETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFF 181

Query: 379 KYRVPEQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSF 432
           KYRVPE+HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS HCSF
Sbjct: 182 KYRVPEEHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSF 227

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL
Match: A0A6J1GRH7 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschata OX=3662 GN=LOC111456372 PE=4 SV=1)

HSP 1 Score: 387 bits (993), Expect = 5.85e-130
Identity = 197/235 (83.83%), Postives = 199/235 (84.68%), Query Frame = 0


Query: 2   ETDPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV 61
           ET PRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV
Sbjct: 4   ETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV 63

Query: 62  FKAVEALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLI-- 121
           FKAVEALF+LPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNI GVQAFTHL+  
Sbjct: 64  FKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHLMWP 123

Query: 122 -------------------------------YGVEKYHETHAASITYLLRALKNKTPHSQ 181
                                          YGVEKYHETHAASITYLLRALKNK PHSQ
Sbjct: 124 NGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQ 183

Query: 182 NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK 203
           NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK
Sbjct: 184 NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK 238

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL
Match: A0A6J1JM78 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima OX=3661 GN=LOC111488145 PE=4 SV=1)

HSP 1 Score: 379 bits (974), Expect = 4.42e-127
Identity = 194/235 (82.55%), Postives = 198/235 (84.26%), Query Frame = 0


Query: 2   ETDPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEV 61
           ET PRMDAGLPVINFSGDDVKAEGGE WRRRSGEVREALEEYGCFIAEYEGISPELRV+V
Sbjct: 4   ETGPRMDAGLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELRVQV 63

Query: 62  FKAVEALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLI-- 121
           FKAVEALF+LPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNI GVQAFT+L+  
Sbjct: 64  FKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNLMWP 123

Query: 122 -------------------------------YGVEKYHETHAASITYLLRALKNKTPHSQ 181
                                          YGVEKYHETHAASITYLLRALKNK PHSQ
Sbjct: 124 NGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQ 183

Query: 182 NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK 203
           NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK
Sbjct: 184 NPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFK 238

[Close]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL
Match: A0A498HFF2 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_031377 PE=4 SV=1)

HSP 1 Score: 357 bits (917), Expect = 1.54e-113
Identity = 208/528 (39.39%), Postives = 290/528 (54.92%), Query Frame = 0


Query: 11  LPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEVFKAVEALFD 70
           LPV++FS + +K  G   W     +V+ ALEE+GCF+  Y+ IS E R     ++E LFD
Sbjct: 28  LPVLDFSKEALKP-GTNSWLLACKDVQRALEEFGCFVVLYDKISDEFRNAFVGSLEELFD 87

Query: 71  LPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLI----------- 130
           LP ETK  N+YEKP NGYVG++  LPLHESLGID ATN    + FT LI           
Sbjct: 88  LPTETKMTNKYEKPLNGYVGQIPKLPLHESLGIDCATNFEETKKFTKLIERAYAFAKVAE 147

Query: 131 -------------YGVEKYHETHAASITYLLRALKNKTPHSQNPTLCLVDHTDKSFTTMV 190
                        YGVEKY++++  SI+YLLR L+N++P      L  V HTDKSFTT++
Sbjct: 148 ELDQMVARMIFESYGVEKYYDSYVGSISYLLRILQNRSPKENEHNLGFVAHTDKSFTTVL 207

Query: 191 YQDH-TNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKV--------------------- 250
           YQ++  N LEV+T++  WI+V+   PS+F+V+AGDA                        
Sbjct: 208 YQNNQVNALEVETRNHEWIKVE-FPPSSFIVMAGDALMAWSNDRILSPNHRVIMSGNETR 267

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 268 YSLAQFAFSDGEIHVPEELGDEERPLRYKSFDHPGLLRFFRSQDRYISNSAIKAYCDRMG 327

Query: 311 -----EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGA 370
                ++PVIDLS  E L+ G+DAW   CK ++  LEE+GCF  ++ ++ LE+HNS F A
Sbjct: 328 SETQPKLPVIDLSD-ENLKPGTDAWLLACKEIKDALEEYGCFEAIYHKIPLELHNSIFSA 387

Query: 371 VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQG 427
           +++LF LP+ET+ + TS++ YH YFG+YSFLPLYES+ +DNPT ++ AQ F +++     
Sbjct: 388 MEDLFGLPLETKKQKTSDRPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTSII----- 447

[Close]

BLAST of Cp4.1LG12g07740 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 193.7 bits (491), Expect = 3.0e-49
Identity = 101/229 (44.10%), Postives = 144/229 (62.88%), Query Frame = 0


Query: 7   MDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISP-ELRVEVFKAV 66
           M   +P ++FS +D+K  G + W      +R+ALEEYGCFI + +  +P +L   VF ++
Sbjct: 1   MSPKIPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSL 60

Query: 67  EALFDLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLI------ 126
             LFDLP +TK KN+Y+KP NGYVG++  LPLHESLGID+AT++   ++FT L+      
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNE 120

Query: 127 ---------------------------YGVEKYHETHAASITYLLRALKNKTPHSQNPTL 186
                                      Y VEKY++ +  S TYLLR LKN+ P+++NPTL
Sbjct: 121 HFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTL 180

Query: 187 CLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDA 202
             V HTDKSFTT+++QD  NGLE++T++G  I ++ SSPS F+V+AGDA
Sbjct: 181 GFVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDA 228

[Close]

BLAST of Cp4.1LG12g07740 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.6e-47
Identity = 89/221 (40.27%), Postives = 137/221 (61.99%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           +VP++DL+  ++L+  +  W S  +     LEE+GCF  V++ V+ ++ +S F A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 265 QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFR 324
            LP ET+ KN +EK YHGY G+   +PL+E + +D  T  + AQ F +LMWP QGNDRF 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF- 128

Query: 325 SLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPE 384
                    T+  FS  +A+LD++V RM+ E+YGV K   ++++ S   LL+F KY  P 
Sbjct: 129 -------CNTVHTFSNAVAELDRLVVRMIFENYGVEKH-YESHVGSKTYLLKFLKYLAPP 188

Query: 385 QHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLE 426
           +  +      HTD TF SI+HQ+ + GL+++S D +WI L+
Sbjct: 189 ESISMPAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQ 219

[Close]

BLAST of Cp4.1LG12g07740 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.9e-44
Identity = 88/224 (39.29%), Postives = 140/224 (62.50%), Query Frame = 0


Query: 204 VEVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDEL 263
           + +PVID S  + L+ G   W  T   ++  L+++G F   F+ +  E+  S FGA++EL
Sbjct: 10  LRLPVIDFSN-KNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEEL 69

Query: 264 FQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRF 323
           F LP++T+++N S+K +HGY G+Y  +PLYESM ID+    +   +F   +WP QGN  F
Sbjct: 70  FDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISF 129

Query: 324 RSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP 383
                   + TIQ FS  +++LD  + RM++ES+G+ K  ID ++ ST  LLR  KY+ P
Sbjct: 130 --------STTIQSFSKKLSELDITIRRMIMESFGLDK-YIDEHLHSTNYLLRVMKYKGP 189

Query: 384 EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS 428
           +  ET VGL+AHTD    +I++Q+ + GL++Q+ D  WI ++P+
Sbjct: 190 DTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPT 222

[Close]

BLAST of Cp4.1LG12g07740 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-40
Identity = 84/229 (36.68%), Postives = 136/229 (59.39%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           ++PVID S  + L+ GS  W      +   LE++GCF   F+++S+E++ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 265 QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRFR 324
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WPD G     
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHG----- 132

Query: 325 SLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP- 384
              N + +ETI  FS  + +LD +V RM++ES+G+ +  ID +++ST  L R  KY  P 
Sbjct: 133 ---NKSISETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPP 192

Query: 385 -----EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPS 428
                +  ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS
Sbjct: 193 DDDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS 227

[Close]

BLAST of Cp4.1LG12g07740 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 3.7e-31
Identity = 77/225 (34.22%), Postives = 118/225 (52.44%), Query Frame = 0


Query: 205 EVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFFGAVDELF 264
           E+P+I+LS  + L+  ++ W  T  ++R  +E  G F   +      +H S   A  EL 
Sbjct: 8   ELPIINLSD-KNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELL 67

Query: 265 QLPIETRIKNTSEKAYHGYFGRYS-FLPLYESMAIDNPTQLDAAQSFANLMWPDQGNDRF 324
            LP+E ++KN + KA HGY    S   P++E + ID    +   + F+ LMWPD  +D  
Sbjct: 68  DLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDD-- 127

Query: 325 RSLLNSNSTETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVP 384
               N    ET+  ++ + A+L+++V RM+ ESY V K   +  I  T  LLR  KYR  
Sbjct: 128 ----NDRFCETVHAYAKMQAELEQLVIRMLFESYNVEK-YTEKYIGGTRYLLRLLKYRRL 187

Query: 385 EQHETDVGLHAHTDLTFFSIIHQHLIGGLQIQS-LDDQWIDLEPS 428
              E +    +HTD +F SI+HQ+ I GL ++S  +D W    PS
Sbjct: 188 PNGEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS 224

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g07740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q944Z5	9.4e-40	37.44	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q945B6	1.2e-39	37.28	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	4.7e-39	36.68	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
A0A0N9HQ36	3.3e-29	33.93	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	3.5e-26	34.36	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Cp4.1LG12g07740 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014111.1	4.14e-254	77.23	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
XP_023547382.1	3.77e-150	93.59	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo]	[more]
KAG6575570.1	2.50e-148	91.88	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
XP_022953943.1	1.01e-147	91.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_022992484.1	7.26e-143	88.46	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG12g07740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPR2	4.90e-148	91.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1JXN6	3.51e-143	88.46	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GRH7	5.85e-130	83.83	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschat...	[more]
A0A6J1JM78	4.42e-127	82.55	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima ...	[more]
A0A498HFF2	1.54e-113	39.39	Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_031377 PE=4 SV=1	[more]

BLAST of Cp4.1LG12g07740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	3.0e-49	44.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	3.6e-47	40.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	2.9e-44	39.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	3.3e-40	36.68	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	3.7e-31	34.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 380..427 e-value: 6.1E-5 score: 23.5 coord: 144..203 e-value: 8.4E-7 score: 29.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 115..204 e-value: 4.2E-13 score: 51.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 205..432 e-value: 2.0E-33 score: 118.0 coord: 1..114 e-value: 2.4E-12 score: 48.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 11..90 e-value: 2.4E-11 score: 44.4 coord: 206..285 e-value: 2.2E-9 score: 38.1
None	No IPR available	PANTHER	PTHR47990:SF153	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 9..119 coord: 205..432 coord: 120..204
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..119 coord: 205..432 coord: 120..204
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 9..203
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 204..430

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g07740.1	Cp4.1LG12g07740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding