Cp4.1LG11g07520 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g07520
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmetalloendoproteinase 2-MMP
LocationCp4.1LG11: 5651661 .. 5652785 (+)
RNA-Seq ExpressionCp4.1LG11g07520
SyntenyCp4.1LG11g07520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCCTTACCCCTGATTTTGGCTCTGTTTTCACTCTCCATTTTGGCTCACCCCCACCCAATCTCCGCCCATATCTTCCCCAACGCCTCCTCAATTCCGTCGTGGCTCAACCGGACCAAATACGCTTGGGACCAATTCCAGAACCTCAAAGGCTGCCGCCCTGGCGATCACCTCACCGGCCTCTCCAATCTCAAGACATACCTTAATAAATTCGGCTACATTCCCAACTCCCCTTCCAATTTCTCCGATCTCTTCGACAATGTTCTCCAATCTGCCCTTTTAACGTATCAGAAGAATTTCAATCTCAACGCCACCGGCTTCCTCGACGACCAAACCCTCGCTCAGATTCAGCTACCCAGATGTGGAATCGCCGATATAATCAACGGCGCTTCTTCTATGAACTCCGGTAGCCCAGTGGCTCATAACGCTACTCGTTTTCACTCTGTTTCTCATTACTCTTTCTTCCCTGGACGTCCTCGCTGGCCTGACCACCGTCGGGATTTGACTTACGCATTTGCGCCGGAGAATGGTCTTACTAATGAGGTTAAAGGGGTTTTCACTAGGGCTTTTGCTCGTTGGGCGGCTGTTACGCCGTTGACGTTCACGGCGGTGGAATCGTTCAGAGCGGCGGATATCCGGATTGGATTCTACGCTGGCGACCATGGCGATGGGGAGCCGTTCGATGGAGTTTTAGGGACTTTGGCTCATGCCTTCTCGCCGCCGAGTGGGCATTTTCATTTGGACAGCGATGAGAGCTGGGTCGTTAACGGCGACCTGAGATCGGCTCCGGTGACGGCGATTGACTTGGAATCGGTAGCGGTTCATGAAATTGGGCATTTATTGGGGCTTGGCCATTCCTCCGTTGAGGAATCCATTATGTTCCCAACAATAACATCGAGGACGAGGAAGGTAGATCTGGCCGTCGACGACGTCACCGGAATTCAAGAATTGTACGGCGCAAACCCAAACGGCAACCCGTCGCCACCGTCGAGTACAACGACGCCACCGTCGTCGGTACAGGAGAGAGATATGTCCAGAAAGGGAGGTGCTCCGAAGGTGTTGAATTCATGGTGGAGCCCACTGGTAGTCGCTGCCGTTGAACTTTTCTTGATGAGACAATAA

mRNA sequence

ATGAAATCCTTACCCCTGATTTTGGCTCTGTTTTCACTCTCCATTTTGGCTCACCCCCACCCAATCTCCGCCCATATCTTCCCCAACGCCTCCTCAATTCCGTCGTGGCTCAACCGGACCAAATACGCTTGGGACCAATTCCAGAACCTCAAAGGCTGCCGCCCTGGCGATCACCTCACCGGCCTCTCCAATCTCAAGACATACCTTAATAAATTCGGCTACATTCCCAACTCCCCTTCCAATTTCTCCGATCTCTTCGACAATGTTCTCCAATCTGCCCTTTTAACGTATCAGAAGAATTTCAATCTCAACGCCACCGGCTTCCTCGACGACCAAACCCTCGCTCAGATTCAGCTACCCAGATGTGGAATCGCCGATATAATCAACGGCGCTTCTTCTATGAACTCCGGTAGCCCAGTGGCTCATAACGCTACTCGTTTTCACTCTGTTTCTCATTACTCTTTCTTCCCTGGACGTCCTCGCTGGCCTGACCACCGTCGGGATTTGACTTACGCATTTGCGCCGGAGAATGGTCTTACTAATGAGGTTAAAGGGGTTTTCACTAGGGCTTTTGCTCGTTGGGCGGCTGTTACGCCGTTGACGTTCACGGCGGTGGAATCGTTCAGAGCGGCGGATATCCGGATTGGATTCTACGCTGGCGACCATGGCGATGGGGAGCCGTTCGATGGAGTTTTAGGGACTTTGGCTCATGCCTTCTCGCCGCCGAGTGGGCATTTTCATTTGGACAGCGATGAGAGCTGGGTCGTTAACGGCGACCTGAGATCGGCTCCGGTGACGGCGATTGACTTGGAATCGGTAGCGGTTCATGAAATTGGGCATTTATTGGGGCTTGGCCATTCCTCCGTTGAGGAATCCATTATGTTCCCAACAATAACATCGAGGACGAGGAAGGTAGATCTGGCCGTCGACGACGTCACCGGAATTCAAGAATTGTACGGCGCAAACCCAAACGGCAACCCGTCGCCACCGTCGAGTACAACGACGCCACCGTCGTCGGTACAGGAGAGAGATATGTCCAGAAAGGGAGGTGCTCCGAAGGTGTTGAATTCATGGTGGAGCCCACTGGTAGTCGCTGCCGTTGAACTTTTCTTGATGAGACAATAA

Coding sequence (CDS)

ATGAAATCCTTACCCCTGATTTTGGCTCTGTTTTCACTCTCCATTTTGGCTCACCCCCACCCAATCTCCGCCCATATCTTCCCCAACGCCTCCTCAATTCCGTCGTGGCTCAACCGGACCAAATACGCTTGGGACCAATTCCAGAACCTCAAAGGCTGCCGCCCTGGCGATCACCTCACCGGCCTCTCCAATCTCAAGACATACCTTAATAAATTCGGCTACATTCCCAACTCCCCTTCCAATTTCTCCGATCTCTTCGACAATGTTCTCCAATCTGCCCTTTTAACGTATCAGAAGAATTTCAATCTCAACGCCACCGGCTTCCTCGACGACCAAACCCTCGCTCAGATTCAGCTACCCAGATGTGGAATCGCCGATATAATCAACGGCGCTTCTTCTATGAACTCCGGTAGCCCAGTGGCTCATAACGCTACTCGTTTTCACTCTGTTTCTCATTACTCTTTCTTCCCTGGACGTCCTCGCTGGCCTGACCACCGTCGGGATTTGACTTACGCATTTGCGCCGGAGAATGGTCTTACTAATGAGGTTAAAGGGGTTTTCACTAGGGCTTTTGCTCGTTGGGCGGCTGTTACGCCGTTGACGTTCACGGCGGTGGAATCGTTCAGAGCGGCGGATATCCGGATTGGATTCTACGCTGGCGACCATGGCGATGGGGAGCCGTTCGATGGAGTTTTAGGGACTTTGGCTCATGCCTTCTCGCCGCCGAGTGGGCATTTTCATTTGGACAGCGATGAGAGCTGGGTCGTTAACGGCGACCTGAGATCGGCTCCGGTGACGGCGATTGACTTGGAATCGGTAGCGGTTCATGAAATTGGGCATTTATTGGGGCTTGGCCATTCCTCCGTTGAGGAATCCATTATGTTCCCAACAATAACATCGAGGACGAGGAAGGTAGATCTGGCCGTCGACGACGTCACCGGAATTCAAGAATTGTACGGCGCAAACCCAAACGGCAACCCGTCGCCACCGTCGAGTACAACGACGCCACCGTCGTCGGTACAGGAGAGAGATATGTCCAGAAAGGGAGGTGCTCCGAAGGTGTTGAATTCATGGTGGAGCCCACTGGTAGTCGCTGCCGTTGAACTTTTCTTGATGAGACAATAA

Protein sequence

MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSPLVVAAVELFLMRQ
Homology
BLAST of Cp4.1LG11g07520 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 7.0e-108
Identity = 206/360 (57.22%), Postives = 250/360 (69.44%), Query Frame = 0

Query: 13  LSILAHPHPISAHIFPNASSI-PSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKTYLNK 72
           LS+     P SA  FPN++++ PS  N T+  WD F N  GC  G ++ GL  +K Y  +
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQR 68

Query: 73  FGYIPNSPS-NFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIING 132
           FGYIP + S NF+D FD++L++A+  YQ NFNLN TG LD  T+  I +PRCG  D++NG
Sbjct: 69  FGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNG 128

Query: 133 ASSMNSGS----PVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGV 192
            S M+ G      V  + T  H+V  Y+ FPG PRWP +RRDLTYAF P+N LT EVK V
Sbjct: 129 TSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSV 188

Query: 193 FTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHF 252
           F+RAF RW+ VT L FT  ESF  +DI IGFY GDHGDGEPFDGVLGTLAHAFSPPSG F
Sbjct: 189 FSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKF 248

Query: 253 HLDSDESWVVNGDLRS--APVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRK 312
           HLD+DE+WVV+GDL S  +   A+DLESVAVHEIGHLLGLGHSSVEESIM+PTIT+  RK
Sbjct: 249 HLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRK 308

Query: 313 VDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQ-ERDMSRKGGAPKVLNSWWSPLV 364
           VDL  DDV GIQ LYGANPN N      TT+PPS+ + +RD      A ++  S  S +V
Sbjct: 309 VDLTNDDVEGIQYLYGANPNFN-----GTTSPPSTTKHQRDTGGFSAAWRIDGSSRSTIV 363

BLAST of Cp4.1LG11g07520 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.5e-106
Identity = 195/343 (56.85%), Postives = 235/343 (68.51%), Query Frame = 0

Query: 19  PHPISAHIFPNASSIPSWL--NRTKYAWDQFQNLKGCRPGDHLTGLSNLKTYLNKFGYI- 78
           P P+SA  + N+S+IP  L  N T   W+ F N  GC  G    GL  LK Y   FGYI 
Sbjct: 16  PSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYIT 75

Query: 79  -PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIINGASSM 138
             N   NF+D FD++L++A+  YQ+NF LN TG LD+ TL  + +PRCG  D++NG S+M
Sbjct: 76  ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTM 135

Query: 139 NSGSPV-----AHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVFTR 198
           +SG        A    RFH+V HYSFFPG PRWP +RRDLTYAF P N LT EVK VF+R
Sbjct: 136 HSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSR 195

Query: 199 AFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLD 258
           AF RW  VTPLTFT VE F  +DI IGFY+G+HGDGEPFDG + TLAHAFSPP+GHFHLD
Sbjct: 196 AFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLD 255

Query: 259 SDESWVVNGDLRSAPVT---AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVD 318
            +E+W+V+G+     ++   A+DLESVAVHEIGHLLGLGHSSVE SIM+PTI +  RKVD
Sbjct: 256 GEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVD 315

Query: 319 LAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKG 350
           L  DDV G+Q LYGANPN N S      +PP S Q+RD    G
Sbjct: 316 LTTDDVEGVQYLYGANPNFNGS-----RSPPPSTQQRDTGDSG 353

BLAST of Cp4.1LG11g07520 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.7e-101
Identity = 196/345 (56.81%), Postives = 241/345 (69.86%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIP--SWLNRTKYAWDQFQNLKGCRPGDH 60
           M++L L + +F  ++    +PISA  + N SSIP   +LN T+ AW+ F  L GC  G++
Sbjct: 1   MRTLLLTILIFFFTV----NPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGEN 60

Query: 61  LTGLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQ 120
           + GLS LK Y  +FGYI  +  N +D FD+VLQSA+ TYQKNFNL  TG LD  TL QI 
Sbjct: 61  INGLSKLKQYFRRFGYI-TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 120

Query: 121 LPRCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENG 180
            PRCG  D+I+G S MN G  +        +   YSFFPG+PRWP  +RDLTYAFAP+N 
Sbjct: 121 KPRCGNPDLIDGVSEMNGGKIL-------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNN 180

Query: 181 LTNEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHA 240
           LT+EVK VF+RAF RWA VTPL FT  ES   ADI IGF++G+HGDGEPFDG +GTLAHA
Sbjct: 181 LTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHA 240

Query: 241 FSPPSGHFHLDSDESWVV-NGDL--RSAPV-TAIDLESVAVHEIGHLLGLGHSSVEESIM 300
            SPP+G  HLD DE W++ NG++  R  PV T +DLESVAVHEIGHLLGLGHSSVE++IM
Sbjct: 241 SSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIM 300

Query: 301 FPTITSRTRKVDLAVDDVTGIQELYGANPNGN-----PSPPSSTT 335
           FP I+   RKV+LA DD+ GIQ LYG NPNG+     PS  S +T
Sbjct: 301 FPAISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQST 333

BLAST of Cp4.1LG11g07520 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.7e-78
Identity = 145/321 (45.17%), Postives = 206/321 (64.17%), Query Frame = 0

Query: 11  FSLSILAHPHPISAHIFPNA----SSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLK 70
           F L +   P+   A   P A    +     ++ +   W  F  L   + G H++G+S LK
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELK 74

Query: 71  TYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIAD 130
            YL++FGY+ +    FSD+FD  L+SA+  YQ+N  L  TG LD  T+  + LPRCG++D
Sbjct: 75  RYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD 134

Query: 131 IINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGL----TNE 190
                      + +  N    H+ +HY++F G+P+W  +R  LTYA +  + L    + +
Sbjct: 135 -----------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSED 194

Query: 191 VKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPP 250
           VK VF RAF++W++V P++F  V+ F  AD++IGFYAGDHGDG PFDGVLGTLAHAF+P 
Sbjct: 195 VKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPE 254

Query: 251 SGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 310
           +G  HLD+ E+W+V+ DL+ +   A+DLESVA HEIGHLLGLGHSS E ++M+P++  RT
Sbjct: 255 NGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRT 314

Query: 311 RKVDLAVDDVTGIQELYGANP 324
           +KVDL VDDV G+ +LYG NP
Sbjct: 315 KKVDLTVDDVAGVLKLYGPNP 322

BLAST of Cp4.1LG11g07520 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 147/297 (49.49%), Postives = 183/297 (61.62%), Query Frame = 0

Query: 30  ASSIPSWLNRTKYAWDQFQNLK--GCRPGDHLTGLSNLKTYLNKFGYIPNSPSNFSDLFD 89
           A+S+PS      YAWD     K     PG +  GLSN+K Y +  GYIPN+P +F D FD
Sbjct: 20  ATSLPSVSAHGPYAWDGEATYKFTTYHPGQNYKGLSNVKNYFHHLGYIPNAP-HFDDNFD 79

Query: 90  NVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIINGASSMNSGSPVAHNATRF 149
           + L SA+ TYQKN+NLN TG  D  TL QI  PRCG+ DII             +  T F
Sbjct: 80  DTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDIIIN----------TNKTTSF 139

Query: 150 HSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVFTRAFARWAAVTPLTFTAVES 209
             +S Y+FF   PRW      LTYAF+PE  L +  K    RAF++W  V  + F    S
Sbjct: 140 GMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTS 199

Query: 210 FRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDSDESWVVNGDLRSAPVT- 269
           +  A+I+I F + +HGD  PFDG  G L HAF+P  G  H D+DE WV +GD+  +PVT 
Sbjct: 200 YETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTS 259

Query: 270 AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAVDDVTGIQELYGANP 324
           A DLESVAVHEIGHLLGLGHSS   +IM+P+I  RTRKV+LA DD+ GI++LYG NP
Sbjct: 260 AFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLYGINP 305

BLAST of Cp4.1LG11g07520 vs. NCBI nr
Match: XP_023546725.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1972), Expect = 1.13e-278
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT
Sbjct: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 374

BLAST of Cp4.1LG11g07520 vs. NCBI nr
Match: KAG7029995.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 758 bits (1957), Expect = 2.19e-276
Identity = 371/374 (99.20%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT
Sbjct: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           +EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLD DESWVV GDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 374

BLAST of Cp4.1LG11g07520 vs. NCBI nr
Match: XP_022946065.1 (metalloendoproteinase 5-MMP-like [Cucurbita moschata])

HSP 1 Score: 756 bits (1952), Expect = 1.27e-275
Identity = 370/374 (98.93%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHL 
Sbjct: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLN 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           +EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLD DESWVV GDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 374

BLAST of Cp4.1LG11g07520 vs. NCBI nr
Match: XP_022999578.1 (metalloendoproteinase 2-MMP [Cucurbita maxima])

HSP 1 Score: 743 bits (1917), Expect = 2.64e-270
Identity = 366/374 (97.86%), Postives = 368/374 (98.40%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKS  +ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCR GDHLT
Sbjct: 1   MKSFAMILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLT 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           +EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLD DESWVV GDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMS KGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMS-KGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 373

BLAST of Cp4.1LG11g07520 vs. NCBI nr
Match: XP_004143787.1 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN51182.1 hypothetical protein Csa_009286 [Cucumis sativus])

HSP 1 Score: 609 bits (1570), Expect = 1.52e-217
Identity = 298/366 (81.42%), Postives = 322/366 (87.98%), Query Frame = 0

Query: 8   LALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKT 67
           L + SLS+L   +PISAHIFPN SSIPSWLN+T  AWDQF+ LKGCRPGD   GLSNLKT
Sbjct: 4   LLITSLSLLLFLNPISAHIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRFNGLSNLKT 63

Query: 68  YLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADI 127
           YLN+FGYIP+SP NF+DLFD +LQSAL TYQ NFNLNATG LDDQTLAQIQLPRCGI DI
Sbjct: 64  YLNRFGYIPSSP-NFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCGIPDI 123

Query: 128 INGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVF 187
           IN ++SMNSG P    +  FHSVSHYSFFPGRP WP HRRDLTYAFAPEN L+NEVK VF
Sbjct: 124 INNSTSMNSGQPTGRQSPHFHSVSHYSFFPGRPIWPAHRRDLTYAFAPENPLSNEVKAVF 183

Query: 188 TRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 247
            RAFARWAAVTPLTF+AVESFR+ADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH
Sbjct: 184 ARAFARWAAVTPLTFSAVESFRSADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 243

Query: 248 LDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 307
           LD DESWVV+GDLRSAP+ AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL
Sbjct: 244 LDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 303

Query: 308 AVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSPLVVAAV 367
           A DD+ GIQELYG NPNGN SPPSS+TTP  SVQER+MSR G AP+ L  WWSP+++AAV
Sbjct: 304 AADDINGIQELYGGNPNGNLSPPSSSTTP--SVQEREMSRNGAAPRPLRFWWSPVLIAAV 363

Query: 368 ELFLMR 373
           ELFLMR
Sbjct: 364 ELFLMR 366

BLAST of Cp4.1LG11g07520 vs. ExPASy TrEMBL
Match: A0A6J1G2R8 (metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 6.13e-276
Identity = 370/374 (98.93%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHL 
Sbjct: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLN 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           +EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLD DESWVV GDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 374

BLAST of Cp4.1LG11g07520 vs. ExPASy TrEMBL
Match: A0A6J1KK37 (metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=1)

HSP 1 Score: 743 bits (1917), Expect = 1.28e-270
Identity = 366/374 (97.86%), Postives = 368/374 (98.40%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLT 60
           MKS  +ILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCR GDHLT
Sbjct: 1   MKSFAMILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLT 60

Query: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120
           GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP
Sbjct: 61  GLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLP 120

Query: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180
           RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT
Sbjct: 121 RCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 180

Query: 181 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240
           +EVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS
Sbjct: 181 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 240

Query: 241 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300
           PPSGHFHLD DESWVV GDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 241 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 300

Query: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 360
           RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMS KGGAPKVLNSWWS
Sbjct: 301 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMS-KGGAPKVLNSWWS 360

Query: 361 PLVVAAVELFLMRQ 374
           PLVVAAVELFLMRQ
Sbjct: 361 PLVVAAVELFLMRQ 373

BLAST of Cp4.1LG11g07520 vs. ExPASy TrEMBL
Match: A0A0A0KRY9 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 7.34e-218
Identity = 298/366 (81.42%), Postives = 322/366 (87.98%), Query Frame = 0

Query: 8   LALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKT 67
           L + SLS+L   +PISAHIFPN SSIPSWLN+T  AWDQF+ LKGCRPGD   GLSNLKT
Sbjct: 4   LLITSLSLLLFLNPISAHIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRFNGLSNLKT 63

Query: 68  YLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADI 127
           YLN+FGYIP+SP NF+DLFD +LQSAL TYQ NFNLNATG LDDQTLAQIQLPRCGI DI
Sbjct: 64  YLNRFGYIPSSP-NFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCGIPDI 123

Query: 128 INGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVF 187
           IN ++SMNSG P    +  FHSVSHYSFFPGRP WP HRRDLTYAFAPEN L+NEVK VF
Sbjct: 124 INNSTSMNSGQPTGRQSPHFHSVSHYSFFPGRPIWPAHRRDLTYAFAPENPLSNEVKAVF 183

Query: 188 TRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 247
            RAFARWAAVTPLTF+AVESFR+ADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH
Sbjct: 184 ARAFARWAAVTPLTFSAVESFRSADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 243

Query: 248 LDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 307
           LD DESWVV+GDLRSAP+ AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL
Sbjct: 244 LDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 303

Query: 308 AVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSPLVVAAV 367
           A DD+ GIQELYG NPNGN SPPSS+TTP  SVQER+MSR G AP+ L  WWSP+++AAV
Sbjct: 304 AADDINGIQELYGGNPNGNLSPPSSSTTP--SVQEREMSRNGAAPRPLRFWWSPVLIAAV 363

Query: 368 ELFLMR 373
           ELFLMR
Sbjct: 364 ELFLMR 366

BLAST of Cp4.1LG11g07520 vs. ExPASy TrEMBL
Match: A0A5D3DJB2 (Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00360 PE=3 SV=1)

HSP 1 Score: 594 bits (1531), Expect = 5.51e-212
Identity = 297/366 (81.15%), Postives = 317/366 (86.61%), Query Frame = 0

Query: 8   LALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKT 67
           L + SLS+L   +PISA IFPN SSIPSWLN+T  AWDQF+ LKGCRPGD L GLSNLKT
Sbjct: 4   LLITSLSLLVFLNPISADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNLKT 63

Query: 68  YLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADI 127
           YLN FGYIP SP NF+DLFD +LQSAL TYQ NFNLNATG LDDQTLAQIQLPRCGI DI
Sbjct: 64  YLNHFGYIPPSP-NFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCGIPDI 123

Query: 128 INGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVF 187
           IN ++SMNSG    H    FHSVSHY+FFPGRP WP HRRDLTYAFAPEN L+NEVK VF
Sbjct: 124 INNSTSMNSGRHSPH----FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLSNEVKAVF 183

Query: 188 TRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 247
            RAFARWAAVTPLTF+AVESFR ADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH
Sbjct: 184 ARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 243

Query: 248 LDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 307
           LD DESWVV+GDLRSAP+ AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL
Sbjct: 244 LDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 303

Query: 308 AVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSPLVVAAV 367
           A DD+ GIQELYG NPNGN SPPSS+ TP  SVQERDMSR G A ++L  WWSPL++AAV
Sbjct: 304 AADDINGIQELYGGNPNGNLSPPSSSMTP--SVQERDMSRNGAAHRLLGFWWSPLLIAAV 362

Query: 368 ELFLMR 373
           ELFLMR
Sbjct: 364 ELFLMR 362

BLAST of Cp4.1LG11g07520 vs. ExPASy TrEMBL
Match: A0A1S3CPI6 (metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1)

HSP 1 Score: 594 bits (1531), Expect = 5.51e-212
Identity = 297/366 (81.15%), Postives = 317/366 (86.61%), Query Frame = 0

Query: 8   LALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKT 67
           L + SLS+L   +PISA IFPN SSIPSWLN+T  AWDQF+ LKGCRPGD L GLSNLKT
Sbjct: 4   LLITSLSLLVFLNPISADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNLKT 63

Query: 68  YLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADI 127
           YLN FGYIP SP NF+DLFD +LQSAL TYQ NFNLNATG LDDQTLAQIQLPRCGI DI
Sbjct: 64  YLNHFGYIPPSP-NFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCGIPDI 123

Query: 128 INGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVF 187
           IN ++SMNSG    H    FHSVSHY+FFPGRP WP HRRDLTYAFAPEN L+NEVK VF
Sbjct: 124 INNSTSMNSGRHSPH----FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLSNEVKAVF 183

Query: 188 TRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 247
            RAFARWAAVTPLTF+AVESFR ADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH
Sbjct: 184 ARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFH 243

Query: 248 LDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 307
           LD DESWVV+GDLRSAP+ AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL
Sbjct: 244 LDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDL 303

Query: 308 AVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWSPLVVAAV 367
           A DD+ GIQELYG NPNGN SPPSS+ TP  SVQERDMSR G A ++L  WWSPL++AAV
Sbjct: 304 AADDINGIQELYGGNPNGNLSPPSSSMTP--SVQERDMSRNGAAHRLLGFWWSPLLIAAV 362

Query: 368 ELFLMR 373
           ELFLMR
Sbjct: 364 ELFLMR 362

BLAST of Cp4.1LG11g07520 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 392.1 bits (1006), Expect = 4.9e-109
Identity = 206/360 (57.22%), Postives = 250/360 (69.44%), Query Frame = 0

Query: 13  LSILAHPHPISAHIFPNASSI-PSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLKTYLNK 72
           LS+     P SA  FPN++++ PS  N T+  WD F N  GC  G ++ GL  +K Y  +
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQR 68

Query: 73  FGYIPNSPS-NFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIING 132
           FGYIP + S NF+D FD++L++A+  YQ NFNLN TG LD  T+  I +PRCG  D++NG
Sbjct: 69  FGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNG 128

Query: 133 ASSMNSGS----PVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGV 192
            S M+ G      V  + T  H+V  Y+ FPG PRWP +RRDLTYAF P+N LT EVK V
Sbjct: 129 TSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSV 188

Query: 193 FTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHF 252
           F+RAF RW+ VT L FT  ESF  +DI IGFY GDHGDGEPFDGVLGTLAHAFSPPSG F
Sbjct: 189 FSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKF 248

Query: 253 HLDSDESWVVNGDLRS--APVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRK 312
           HLD+DE+WVV+GDL S  +   A+DLESVAVHEIGHLLGLGHSSVEESIM+PTIT+  RK
Sbjct: 249 HLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRK 308

Query: 313 VDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQ-ERDMSRKGGAPKVLNSWWSPLV 364
           VDL  DDV GIQ LYGANPN N      TT+PPS+ + +RD      A ++  S  S +V
Sbjct: 309 VDLTNDDVEGIQYLYGANPNFN-----GTTSPPSTTKHQRDTGGFSAAWRIDGSSRSTIV 363

BLAST of Cp4.1LG11g07520 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.6e-107
Identity = 195/343 (56.85%), Postives = 235/343 (68.51%), Query Frame = 0

Query: 19  PHPISAHIFPNASSIPSWL--NRTKYAWDQFQNLKGCRPGDHLTGLSNLKTYLNKFGYI- 78
           P P+SA  + N+S+IP  L  N T   W+ F N  GC  G    GL  LK Y   FGYI 
Sbjct: 16  PSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYIT 75

Query: 79  -PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIADIINGASSM 138
             N   NF+D FD++L++A+  YQ+NF LN TG LD+ TL  + +PRCG  D++NG S+M
Sbjct: 76  ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTM 135

Query: 139 NSGSPV-----AHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLTNEVKGVFTR 198
           +SG        A    RFH+V HYSFFPG PRWP +RRDLTYAF P N LT EVK VF+R
Sbjct: 136 HSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSR 195

Query: 199 AFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLD 258
           AF RW  VTPLTFT VE F  +DI IGFY+G+HGDGEPFDG + TLAHAFSPP+GHFHLD
Sbjct: 196 AFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLD 255

Query: 259 SDESWVVNGDLRSAPVT---AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVD 318
            +E+W+V+G+     ++   A+DLESVAVHEIGHLLGLGHSSVE SIM+PTI +  RKVD
Sbjct: 256 GEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVD 315

Query: 319 LAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKG 350
           L  DDV G+Q LYGANPN N S      +PP S Q+RD    G
Sbjct: 316 LTTDDVEGVQYLYGANPNFNGS-----RSPPPSTQQRDTGDSG 353

BLAST of Cp4.1LG11g07520 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.2e-102
Identity = 196/345 (56.81%), Postives = 241/345 (69.86%), Query Frame = 0

Query: 1   MKSLPLILALFSLSILAHPHPISAHIFPNASSIP--SWLNRTKYAWDQFQNLKGCRPGDH 60
           M++L L + +F  ++    +PISA  + N SSIP   +LN T+ AW+ F  L GC  G++
Sbjct: 1   MRTLLLTILIFFFTV----NPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGEN 60

Query: 61  LTGLSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQ 120
           + GLS LK Y  +FGYI  +  N +D FD+VLQSA+ TYQKNFNL  TG LD  TL QI 
Sbjct: 61  INGLSKLKQYFRRFGYI-TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 120

Query: 121 LPRCGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENG 180
            PRCG  D+I+G S MN G  +        +   YSFFPG+PRWP  +RDLTYAFAP+N 
Sbjct: 121 KPRCGNPDLIDGVSEMNGGKIL-------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNN 180

Query: 181 LTNEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHA 240
           LT+EVK VF+RAF RWA VTPL FT  ES   ADI IGF++G+HGDGEPFDG +GTLAHA
Sbjct: 181 LTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHA 240

Query: 241 FSPPSGHFHLDSDESWVV-NGDL--RSAPV-TAIDLESVAVHEIGHLLGLGHSSVEESIM 300
            SPP+G  HLD DE W++ NG++  R  PV T +DLESVAVHEIGHLLGLGHSSVE++IM
Sbjct: 241 SSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIM 300

Query: 301 FPTITSRTRKVDLAVDDVTGIQELYGANPNGN-----PSPPSSTT 335
           FP I+   RKV+LA DD+ GIQ LYG NPNG+     PS  S +T
Sbjct: 301 FPAISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQST 333

BLAST of Cp4.1LG11g07520 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 292.7 bits (748), Expect = 4.1e-79
Identity = 145/321 (45.17%), Postives = 206/321 (64.17%), Query Frame = 0

Query: 11  FSLSILAHPHPISAHIFPNA----SSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNLK 70
           F L +   P+   A   P A    +     ++ +   W  F  L   + G H++G+S LK
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELK 74

Query: 71  TYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCGIAD 130
            YL++FGY+ +    FSD+FD  L+SA+  YQ+N  L  TG LD  T+  + LPRCG++D
Sbjct: 75  RYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD 134

Query: 131 IINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGL----TNE 190
                      + +  N    H+ +HY++F G+P+W  +R  LTYA +  + L    + +
Sbjct: 135 -----------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSED 194

Query: 191 VKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPP 250
           VK VF RAF++W++V P++F  V+ F  AD++IGFYAGDHGDG PFDGVLGTLAHAF+P 
Sbjct: 195 VKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPE 254

Query: 251 SGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 310
           +G  HLD+ E+W+V+ DL+ +   A+DLESVA HEIGHLLGLGHSS E ++M+P++  RT
Sbjct: 255 NGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRT 314

Query: 311 RKVDLAVDDVTGIQELYGANP 324
           +KVDL VDDV G+ +LYG NP
Sbjct: 315 KKVDLTVDDVAGVLKLYGPNP 322

BLAST of Cp4.1LG11g07520 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 251.9 bits (642), Expect = 8.0e-67
Identity = 134/269 (49.81%), Postives = 173/269 (64.31%), Query Frame = 0

Query: 62  LSNLKTYLNKFGYIPNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPR 121
           +  +K +L ++GY+P +    SD  D   + AL+ YQKN  L  TG  D  TL+QI LPR
Sbjct: 50  IPEIKRHLQQYGYLPQNKE--SD--DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 122 CGIADIINGASSMNSGSPVAHNATRFHSVSHYSFFPGRPRWP-DHRRDLTYAFAPENGLT 181
           CG  D             V      FH+   Y +FPGRPRW  D    LTYAF+ EN LT
Sbjct: 110 CGFPD------------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQEN-LT 169

Query: 182 -----NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTL 241
                 +++ VF RAF +WA+V P++F   E +  ADI+IGF+ GDHGDGEPFDGVLG L
Sbjct: 170 PYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVL 229

Query: 242 AHAFSPPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMF 301
           AH FSP +G  HLD  E+W V+ D   + V A+DLESVAVHEIGH+LGLGHSSV+++ M+
Sbjct: 230 AHTFSPENGRLHLDKAETWAVDFDEEKSSV-AVDLESVAVHEIGHVLGLGHSSVKDAAMY 289

Query: 302 PTITSRTRKVDLAVDDVTGIQELYGANPN 325
           PT+  R++KV+L +DDV G+Q LYG NPN
Sbjct: 290 PTLKPRSKKVNLNMDDVVGVQSLYGTNPN 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O045297.0e-10857.22Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q5XF516.5e-10656.85Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q9ZUJ51.7e-10156.81Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O235075.7e-7845.17Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
P291361.1e-7349.49Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023546725.11.13e-278100.00metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo][more]
KAG7029995.12.19e-27699.20Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022946065.11.27e-27598.93metalloendoproteinase 5-MMP-like [Cucurbita moschata][more]
XP_022999578.12.64e-27097.86metalloendoproteinase 2-MMP [Cucurbita maxima][more]
XP_004143787.11.52e-21781.42metalloendoproteinase 2-MMP [Cucumis sativus] >KGN51182.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
A0A6J1G2R86.13e-27698.93metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 P... [more]
A0A6J1KK371.28e-27097.86metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=... [more]
A0A0A0KRY97.34e-21881.42ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 S... [more]
A0A5D3DJB25.51e-21281.15Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CPI65.51e-21281.15metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70170.14.9e-10957.22matrix metalloproteinase [more]
AT1G24140.14.6e-10756.85Matrixin family protein [more]
AT1G59970.11.2e-10256.81Matrixin family protein [more]
AT4G16640.14.1e-7945.17Matrixin family protein [more]
AT2G45040.18.0e-6749.81Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 117..130
score: 38.68
coord: 211..239
score: 61.69
coord: 273..298
score: 56.85
coord: 187..202
score: 52.74
coord: 307..320
score: 63.85
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 158..321
e-value: 7.3E-48
score: 175.0
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 162..320
e-value: 4.8E-48
score: 163.0
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 52..326
e-value: 2.1E-76
score: 258.6
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 63..116
e-value: 7.3E-11
score: 42.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 319..349
NoneNo IPR availablePANTHERPTHR10201:SF260MATRIX METALLOPROTEINASEcoord: 22..357
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 22..357
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 155..324
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 161..320
e-value: 2.98079E-66
score: 205.131
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 53..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g07520.1Cp4.1LG11g07520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity