Cp4.1LG09g10460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g10460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionanaphase-promoting complex subunit 8
LocationCp4.1LG09: 9171665 .. 9175198 (+)
RNA-Seq ExpressionCp4.1LG09g10460
SyntenyCp4.1LG09g10460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTTTGTTTCTTTTTAAAAGTTTGGGCGGGCAGGAGCCGGAGCCTCCGAATATGGTGGGGCGAAACGAACAGAAGGCGCATGTTAGCGACGCTCACAATTTGAAGTTTGAGTTACAAGGAAGAGCGCTAGAAGGGCGCCATAGGAGCGGAGATTCAATGAGTTCCAAAGAAAATTGTAGAAACGAGCTTCGTACTGCGATTCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCTAAATGGTACAATCCCCATAGCCCTAAAATTTCTCTGTGTATCTGTTTTGGTGCTTCTGATCTGTTATTCGCTATCAATCTTGTTTGAACTTCTCAAATGCTGGTTTATGTGAATCATTTTGATCTTCGGAGTCCATTTTCTTGGAGTTTAGATGTTAATTTCACATGATTTCTGAGAAATCAGCGGGTAATAACGAATTGATGATGTTGAATGGATGAATGTTCTTGATTAGCCCTATACTTTTGAAACAATCGGCAAGCTCTTGTAACGATTAATCTCTGTATATTGTAATGTAGCTTAAAGATTTCATTGAAGTTTTGACATGTCTGTATCTTCACAGGGCAGCTGAGCAATTGGTCGGTATTGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACGAGGTTTCAGCGTGGTAGTTCTAGTATTCGTCGACGATTTCACACGAATGAGGGTTGTTCAACCCCAATTACTGGCTTATCTTATGTATCTACTCCGGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAGTCGTATTTTGATTGTCGCGAGTATAATAGGGCGGCTCATGTTCTTCGGGAGCAAAATGGAAAGAAATCCGTGTTCTTGCGGCTGTATGCTCTCTATCTGGTTCGTTAATCCTTACCATTTGTAATGTTTGTTCCTGATTTTAAGTTCTTGTTATGCATCGTCGTAAAGTAATCTGACTCGATTGCGTCGGTCCATAAATATTCAGTTGCCATGATATCTAGTATATGTTTAAATTTTGTTACGTTGATGAATAGAGCCCGTTTTGGTATTACTCAAAAACTTATACGTAATTCTAATAACAAAAACTTATTTGCATCACTATAGTGCCTATTCATGAGCTTTATCACGGTGTAACAGTTCAAGCCCACTGCGAACAAATATTATCTGCTTTAGCTCGTTATGTATGTCATCAGCCTCACGGTTTTAAAACGCGTCTGTTAGGGAGAGGTTTCCACACCCTTATAAGGAATGCTTCGTTCTCCTCTCCAACCGATGTGGGATTTCACAATCCAATCCCCTTGGGGGCATAACGTCCTCGTTGGCACACCGCTTGGTGTCTGGCTCTAATACCATTTGTAACAGCCTAAGCCCACCGCTAACGATATTATCCGCTTTGGCCTGTTACGTTTCGTCGTTAGCTTGACGTGTTTAAAATCGTGAGGCTGACGACGATACGTAATGGGCTAAAGTAGATAATGCTATCTGTTATAGCATATAAGGGTTCTAAGGCTTTTCTTTTACCGTTCGATGAATGTTTTGACGTTTCTTGTTTTCATGTTCTATATACTCGCACATATATTTCTTGGTTTCTCTTCTTCTTCAACTTAAAAGCTCCTGCACAAGCTGACAACCGGTGTCTTACTTCGTGTAGGCTGGTGAAAAGCGGAAAGAAGAAGAGGTAGTTGAACTCGAGGGATCATTAGGTAAGAGCGATGCTGTAAACCAAGAATTGGTTTCACTGGAAAGGGAACTATCAACCCTCCGAAAAAACGTCACAGCTGACCCATTTGAACTCTACTTATACGGACTCGTACTCAAACAGAAAGGCAGTGAAAATCTTTCGCGCACCGTTCTTGTGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCGTTATGCACCACAATTGACATATTGAACAGCCTAAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCGGTGCTTACCAAGAACTTAGAATGCACAATGAATCCTTGGCTAAGTATGAGAACTTACAAGCTACATTTAGTTTTAGTAATTACATTCAAGCTCAAATTGCAAAAGCTCAATATAGTTTAAGGGAGTTCGATCAAGTCGAAGCAATATTCGAAGAACTTCTTCGGAACGATCCATATCGAGTGGAAGACATGGATATGTATTCAAACGTGCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACGGATAAGTATAGACCCGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAAGGACAGCACGAGAAGTCGGTTGTATATTTCCGAAGGGCACTCAAATTGAACAAGAATTATTTATCTGCTTGGACTCTGATGGGACATGAATTCGTGGAGATGAAGAACATCCCGGCTGCTATCGATGCGTATCGAAGGGCTGTAGATATAAATTCATGTGATTATCGAGCTTGGTATGGGTTAGGACAAGCTTACGAAATGATGGGTATGCCCTTTTATGCTCTCCATTACTTCAAGAAGGCTGTATTTTTGCAGCCAAACGATTCTCGGGTTTGGATTGCCATGGCTCAATGTTACGAAAGCGAACAGCTCCGAATGCTCGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAATTGTAATGATAGGGAAGCAATTGCTCTGCACCAGTTGGCTAAACTACACTCTGAGTTGGGGCAGTCGGAAGAGGCTGCATTCTACTACAAGAAGGATTTAGAGAGAATGGAAGCTGAAGAGAGAGAAGGACCAAATATGGTTGAAGCTCTGCTTTTTCTTGCTACATATTATAAAGCACAAAAGAAGTTCGATGAAGCCGAAGTATATTGTACACGGCTCTTGGATTACACCGGGCCGGTGAGTATTACTGGTTTTGTGCATGCTTCGTTCAACGACACGTAATTCGAGTCTCACTCAACAACGTTTTCTTTTGTGTCTTTCGTTGCAGGAGAAGGAAACGGCTAAGGATTTACTTAGAGGAATGAGGCTAGCACAATCTAGCTTTCCATCCATGGATGTCGAGCTCTTTCCTCCGTAAGTTATCTCGGTTGCGACGCCTGATTTTCTAACCTCTTTATTCCAACATTCAGAAACAAATACAGGTCGGTCCATACGGAATAATATATACTGCAAAGTGTTCGGCCTAGCGATCTGCATTTCGTTGAAGTTCCAACGTCGAGGTTCGATGCTTTTATCCTATTGTATTCGTCTTACTCGTTAGCTCAGCTCAGCCCATCCTAGGCTTGGCTGTTCATTCTGAAATGCTCAGTAGAAAGAGCATTTTGATGCCTTGTGGAATCTGTTTTCTGTTGTATCATTGTCGATTGATTCATGTAGCGTTAGAAACTATACTGATTATTTAGAACTGCATTTTTTGTGATCATTCTATTGTGCAATACAAGAAGGGCAATTTGTGCACATTGGGAAAGAATGGTGTAATGGGTAACTTTTGAGCTTTTTTTGGTTAAACCATCGTAGATAC

mRNA sequence

TAATTTTGTTTCTTTTTAAAAGTTTGGGCGGGCAGGAGCCGGAGCCTCCGAATATGGTGGGGCGAAACGAACAGAAGGCGCATGTTAGCGACGCTCACAATTTGAAGTTTGAGTTACAAGGAAGAGCGCTAGAAGGGCGCCATAGGAGCGGAGATTCAATGAGTTCCAAAGAAAATTGTAGAAACGAGCTTCGTACTGCGATTCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCTAAATGGGCAGCTGAGCAATTGGTCGGTATTGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACGAGGTTTCAGCGTGGTAGTTCTAGTATTCGTCGACGATTTCACACGAATGAGGGTTGTTCAACCCCAATTACTGGCTTATCTTATGTATCTACTCCGGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAGTCGTATTTTGATTGTCGCGAGTATAATAGGGCGGCTCATGTTCTTCGGGAGCAAAATGGAAAGAAATCCGTGTTCTTGCGGCTGTATGCTCTCTATCTGGCTGGTGAAAAGCGGAAAGAAGAAGAGGTAGTTGAACTCGAGGGATCATTAGGTAAGAGCGATGCTGTAAACCAAGAATTGGTTTCACTGGAAAGGGAACTATCAACCCTCCGAAAAAACGTCACAGCTGACCCATTTGAACTCTACTTATACGGACTCGTACTCAAACAGAAAGGCAGTGAAAATCTTTCGCGCACCGTTCTTGTGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCGTTATGCACCACAATTGACATATTGAACAGCCTAAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCGGTGCTTACCAAGAACTTAGAATGCACAATGAATCCTTGGCTAAGTATGAGAACTTACAAGCTACATTTAGTTTTAGTAATTACATTCAAGCTCAAATTGCAAAAGCTCAATATAGTTTAAGGGAGTTCGATCAAGTCGAAGCAATATTCGAAGAACTTCTTCGGAACGATCCATATCGAGTGGAAGACATGGATATGTATTCAAACGTGCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACGGATAAGTATAGACCCGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAAGGACAGCACGAGAAGTCGGTTGTATATTTCCGAAGGGCACTCAAATTGAACAAGAATTATTTATCTGCTTGGACTCTGATGGGACATGAATTCGTGGAGATGAAGAACATCCCGGCTGCTATCGATGCGTATCGAAGGGCTGTAGATATAAATTCATGTGATTATCGAGCTTGGTATGGGTTAGGACAAGCTTACGAAATGATGGGTATGCCCTTTTATGCTCTCCATTACTTCAAGAAGGCTGTATTTTTGCAGCCAAACGATTCTCGGGTTTGGATTGCCATGGCTCAATGTTACGAAAGCGAACAGCTCCGAATGCTCGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAATTGTAATGATAGGGAAGCAATTGCTCTGCACCAGTTGGCTAAACTACACTCTGAGTTGGGGCAGTCGGAAGAGGCTGCATTCTACTACAAGAAGGATTTAGAGAGAATGGAAGCTGAAGAGAGAGAAGGACCAAATATGGTTGAAGCTCTGCTTTTTCTTGCTACATATTATAAAGCACAAAAGAAGTTCGATGAAGCCGAAGTATATTGTACACGGCTCTTGGATTACACCGGGCCGGAGAAGGAAACGGCTAAGGATTTACTTAGAGGAATGAGGCTAGCACAATCTAGCTTTCCATCCATGGATGTCGAGCTCTTTCCTCCGTAAGTTATCTCGGTTGCGACGCCTGATTTTCTAACCTCTTTATTCCAACATTCAGAAACAAATACAGGTCGGTCCATACGGAATAATATATACTGCAAAGTGTTCGGCCTAGCGATCTGCATTTCGTTGAAGTTCCAACGTCGAGGTTCGATGCTTTTATCCTATTGTATTCGTCTTACTCGTTAGCTCAGCTCAGCCCATCCTAGGCTTGGCTGTTCATTCTGAAATGCTCAGTAGAAAGAGCATTTTGATGCCTTGTGGAATCTGTTTTCTGTTGTATCATTGTCGATTGATTCATGTAGCGTTAGAAACTATACTGATTATTTAGAACTGCATTTTTTGTGATCATTCTATTGTGCAATACAAGAAGGGCAATTTGTGCACATTGGGAAAGAATGGTGTAATGGGTAACTTTTGAGCTTTTTTTGGTTAAACCATCGTAGATAC

Coding sequence (CDS)

ATGGTGGGGCGAAACGAACAGAAGGCGCATGTTAGCGACGCTCACAATTTGAAGTTTGAGTTACAAGGAAGAGCGCTAGAAGGGCGCCATAGGAGCGGAGATTCAATGAGTTCCAAAGAAAATTGTAGAAACGAGCTTCGTACTGCGATTCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCTAAATGGGCAGCTGAGCAATTGGTCGGTATTGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACGAGGTTTCAGCGTGGTAGTTCTAGTATTCGTCGACGATTTCACACGAATGAGGGTTGTTCAACCCCAATTACTGGCTTATCTTATGTATCTACTCCGGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAGTCGTATTTTGATTGTCGCGAGTATAATAGGGCGGCTCATGTTCTTCGGGAGCAAAATGGAAAGAAATCCGTGTTCTTGCGGCTGTATGCTCTCTATCTGGCTGGTGAAAAGCGGAAAGAAGAAGAGGTAGTTGAACTCGAGGGATCATTAGGTAAGAGCGATGCTGTAAACCAAGAATTGGTTTCACTGGAAAGGGAACTATCAACCCTCCGAAAAAACGTCACAGCTGACCCATTTGAACTCTACTTATACGGACTCGTACTCAAACAGAAAGGCAGTGAAAATCTTTCGCGCACCGTTCTTGTGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCGTTATGCACCACAATTGACATATTGAACAGCCTAAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCGGTGCTTACCAAGAACTTAGAATGCACAATGAATCCTTGGCTAAGTATGAGAACTTACAAGCTACATTTAGTTTTAGTAATTACATTCAAGCTCAAATTGCAAAAGCTCAATATAGTTTAAGGGAGTTCGATCAAGTCGAAGCAATATTCGAAGAACTTCTTCGGAACGATCCATATCGAGTGGAAGACATGGATATGTATTCAAACGTGCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACGGATAAGTATAGACCCGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAAGGACAGCACGAGAAGTCGGTTGTATATTTCCGAAGGGCACTCAAATTGAACAAGAATTATTTATCTGCTTGGACTCTGATGGGACATGAATTCGTGGAGATGAAGAACATCCCGGCTGCTATCGATGCGTATCGAAGGGCTGTAGATATAAATTCATGTGATTATCGAGCTTGGTATGGGTTAGGACAAGCTTACGAAATGATGGGTATGCCCTTTTATGCTCTCCATTACTTCAAGAAGGCTGTATTTTTGCAGCCAAACGATTCTCGGGTTTGGATTGCCATGGCTCAATGTTACGAAAGCGAACAGCTCCGAATGCTCGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAATTGTAATGATAGGGAAGCAATTGCTCTGCACCAGTTGGCTAAACTACACTCTGAGTTGGGGCAGTCGGAAGAGGCTGCATTCTACTACAAGAAGGATTTAGAGAGAATGGAAGCTGAAGAGAGAGAAGGACCAAATATGGTTGAAGCTCTGCTTTTTCTTGCTACATATTATAAAGCACAAAAGAAGTTCGATGAAGCCGAAGTATATTGTACACGGCTCTTGGATTACACCGGGCCGGAGAAGGAAACGGCTAAGGATTTACTTAGAGGAATGAGGCTAGCACAATCTAGCTTTCCATCCATGGATGTCGAGCTCTTTCCTCCGTAA

Protein sequence

MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFPP
Homology
BLAST of Cp4.1LG09g10460 vs. ExPASy Swiss-Prot
Match: Q9STS3 (Anaphase-promoting complex subunit 8 OS=Arabidopsis thaliana OX=3702 GN=APC8 PE=1 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 1.0e-273
Identity = 457/578 (79.07%), Postives = 516/578 (89.27%), Query Frame = 0

Query: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95
           M SKE CRNE+R AIRQLSDRCLYSA+KWA EQLVGIEQDP+ FTP+NTRFQRGSSSIRR
Sbjct: 1   MVSKECCRNEIRAAIRQLSDRCLYSAAKWAGEQLVGIEQDPSNFTPANTRFQRGSSSIRR 60

Query: 96  RFHTNEGCSTPI--TGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNG 155
           RF TNE  STP+   G S  +TP+ EEDE +DGD YLLAKSYFDCREY RA+H+LR+Q  
Sbjct: 61  RFSTNESISTPLPSVGFSQAATPLPEEDEAIDGDIYLLAKSYFDCREYRRASHMLRDQVS 120

Query: 156 KKSVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFE 215
           KKS+FLR YALYLAGEKRKEEE++ELEG LGKSDA+N+ELVSLER+LS LR+    D F 
Sbjct: 121 KKSLFLRYYALYLAGEKRKEEEMIELEGPLGKSDAINRELVSLERDLSALRRTGAIDSFG 180

Query: 216 LYLYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKD 275
           LYLYG+VLK+KG+E+L+R  LVESVNSYPWNWSAWSELQSLCT+I+ILNSLNLNNHWMK+
Sbjct: 181 LYLYGVVLKEKGNESLARASLVESVNSYPWNWSAWSELQSLCTSIEILNSLNLNNHWMKE 240

Query: 276 FFLAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 335
           FFL  AYQELRMH ESLAKYE LQ  FSFSNYIQAQ AKAQYSLREFDQVE +FEELLRN
Sbjct: 241 FFLGNAYQELRMHTESLAKYEYLQGIFSFSNYIQAQTAKAQYSLREFDQVEIMFEELLRN 300

Query: 336 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 395
           DPYRVEDMD+YSNVLYAKE  +ALSYLAH+VF+TDKYRPESCCIIGNYYSLKGQHEK+V+
Sbjct: 301 DPYRVEDMDLYSNVLYAKEACAALSYLAHKVFLTDKYRPESCCIIGNYYSLKGQHEKAVM 360

Query: 396 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 455
           YFRRALKLNK YLSAWTLMGHE+VEMKN PAAIDAYRRAVDIN  DYRAWYGLGQAYEMM
Sbjct: 361 YFRRALKLNKKYLSAWTLMGHEYVEMKNTPAAIDAYRRAVDINPTDYRAWYGLGQAYEMM 420

Query: 456 GMPFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIAL 515
           GMPFYALHYF+K++F  PNDSR+WIAMA+CY++EQL MLEEAIKCY+RA NC D E IAL
Sbjct: 421 GMPFYALHYFRKSIFFLPNDSRLWIAMAKCYQTEQLYMLEEAIKCYKRAVNCTDTEGIAL 480

Query: 516 HQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVY 575
           +QLAKLH +LG++EEAA+Y++KDLERM+AE  EGPNM EAL+FLAT++K  KKF+EAEVY
Sbjct: 481 NQLAKLHQKLGRNEEAAYYFEKDLERMDAEGLEGPNMFEALVFLATHFKNHKKFEEAEVY 540

Query: 576 CTRLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFP 612
           CTRLLDY+GPEKE AK LLRG+R+AQ+ FPSMD+E FP
Sbjct: 541 CTRLLDYSGPEKEKAKSLLRGIRMAQTGFPSMDLEHFP 578

BLAST of Cp4.1LG09g10460 vs. ExPASy Swiss-Prot
Match: Q86B11 (Anaphase-promoting complex subunit 8 OS=Dictyostelium discoideum OX=44689 GN=anapc8 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.4e-128
Identity = 261/597 (43.72%), Postives = 368/597 (61.64%), Query Frame = 0

Query: 45  ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCS 104
           EL  +I  L+ R L  +SKW++EQL G+                 S +I     TNE   
Sbjct: 7   ELIKSINDLNSRGLLLSSKWSSEQLNGL-----------------SPTILATPLTNE--- 66

Query: 105 TPITGLSYVSTPVMEEDEVVDGDF--YLLAKSYFDCREYNRAAHVLREQNG-KKSVFLRL 164
                LS +S P +    +   ++  Y+LAK+YFD +EY R + VL + N  +  +FLR 
Sbjct: 67  ---EQLSIISQPSISSPPIGSNEYYKYILAKNYFDLKEYRRCSDVLIDCNKYQLPIFLRS 126

Query: 165 YALYLAGEKRKEEEVVELEG--------SLGKSDAVNQELVSLERELSTLRKNVT----- 224
           YA YLA EKR+EE+++E +         +  ++    QE    ++   T   N T     
Sbjct: 127 YATYLAIEKRREEDIIEQQAQQQQQQQQAQQQAQQAQQESQQNDKNNDTNNNNKTDQQQQ 186

Query: 225 ------------------------------ADPFELYLYGLVLKQKGSENLSRTVLVESV 284
                                          D F LY Y ++LK++    ++R VL+ESV
Sbjct: 187 QQQQQKIEQCQEFKQLFQFYKKLYIENKKDMDGFLLYFYSMLLKKQRDFTMARKVLIESV 246

Query: 285 NSYPWNWSAWSELQSLCT-TIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQ 344
           + YP NWSAWS+L SLC+ + DI+  L+L +H+MKDFFLA    EL+ +NESL  Y+ L 
Sbjct: 247 HKYPCNWSAWSDLSSLCSDSADIIMQLSLPDHFMKDFFLAHFKLELQQNNESLVIYQQLS 306

Query: 345 AT-FSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSA 404
            T F+ S YI AQ A   Y+LR +D  E +FE L+  +P R+E++D+YSN+LY ++  ++
Sbjct: 307 RTLFTQSTYILAQTAIGNYNLRAYDIGEELFERLIELEPNRLENIDIYSNILYVRDKKAS 366

Query: 405 LSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEF 464
           LS LAH+    +KY PE+CCIIGNYYSLK +H+K+++YF+RALKLN  YLSAWTL+GHEF
Sbjct: 367 LSMLAHKAMKIEKYCPETCCIIGNYYSLKLEHDKAILYFQRALKLNDRYLSAWTLIGHEF 426

Query: 465 VEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRV 524
           +E+KN+ AAI+AYR+AVDIN  DYRAWYGLGQ Y+++ +P Y+L+YFKKA  L+P D R+
Sbjct: 427 LEIKNVSAAINAYRKAVDINPRDYRAWYGLGQTYQLLKLPLYSLYYFKKATTLRPYDPRM 486

Query: 525 WIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKD 584
           W A   CYE   +  + EAIKCY RA    DRE +A+++LAKL+ E+  +E+AAFYYKK+
Sbjct: 487 WCAAGGCYEF--IERIPEAIKCYERAEENYDRERVAINKLAKLYQEIQNNEKAAFYYKKN 546

Query: 585 LERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLR 594
           L   + E+ +G  +++ALLFLA +YK Q +  ++E YC RLLDY GPEKE AK +LR
Sbjct: 547 LYYCDQEKIDGQEIIDALLFLANFYKNQNQ-TQSEQYCLRLLDYAGPEKEEAKSILR 577

BLAST of Cp4.1LG09g10460 vs. ExPASy Swiss-Prot
Match: A1A4R8 (Cell division cycle protein 23 homolog OS=Bos taurus OX=9913 GN=CDC23 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.4e-118
Identity = 232/481 (48.23%), Postives = 316/481 (65.70%), Query Frame = 0

Query: 116 PVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEE 175
           P + E++  D D Y LAK+YFD +EY+RAAH L   N KK+ FL +Y+ YL+GEK+K++E
Sbjct: 74  PPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 176 VVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLV 235
            V+  G L K    N+ L  L  ELS   +    D F LYLYG+VL++      +  V V
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 236 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYEN 295
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA  Y EL++  E+L KY+N
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQN 253

Query: 296 L-QATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 355
           L    FS S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 356 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 415
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 416 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDS 475
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y+++A  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 476 RVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYK 535
           R+ +A+ +CY  E+L  L EA KCY RA    D E +AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYI 493

Query: 536 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 594
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASA-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG09g10460 vs. ExPASy Swiss-Prot
Match: Q9UJX2 (Cell division cycle protein 23 homolog OS=Homo sapiens OX=9606 GN=CDC23 PE=1 SV=3)

HSP 1 Score: 427.6 bits (1098), Expect = 2.4e-118
Identity = 232/481 (48.23%), Postives = 316/481 (65.70%), Query Frame = 0

Query: 116 PVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEE 175
           P + E++  D D Y LAK+YFD +EY+RAAH L   N KK+ FL +Y+ YL+GEK+K++E
Sbjct: 74  PPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 176 VVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLV 235
            V+  G L K    N+ L  L  ELS   +    D F LYLYG+VL++      +  V V
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 236 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYEN 295
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA  Y EL++  E+L KY+N
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQN 253

Query: 296 L-QATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 355
           L    FS S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 356 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 415
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 416 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDS 475
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y+++A  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 476 RVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYK 535
           R+ +A+ +CY  E+L  L EA KCY RA    D E +AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYI 493

Query: 536 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 594
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEAST-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG09g10460 vs. ExPASy Swiss-Prot
Match: Q8BGZ4 (Cell division cycle protein 23 homolog OS=Mus musculus OX=10090 GN=Cdc23 PE=1 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 1.6e-117
Identity = 231/481 (48.02%), Postives = 315/481 (65.49%), Query Frame = 0

Query: 116 PVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEE 175
           P + E++  D D Y LAK+YFD +EY+RAAH L   N KK+ FL +Y+ YL+GEK+K++E
Sbjct: 74  PPLTEEDAQDVDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 176 VVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLV 235
            V+  G L K    N+ L  L  ELS   +    D F LYLYG+VL++      +  V V
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 236 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYEN 295
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA  Y EL++  E+L KY++
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQH 253

Query: 296 L-QATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 355
           L    FS S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 356 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 415
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 416 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDS 475
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y+++A  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 476 RVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYK 535
           R+ +A+ +CY  E+L  L EA KCY RA    D E  AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKKALVKLAKLHEQLTESEQAAQCYI 493

Query: 536 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 594
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEAST-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG09g10460 vs. NCBI nr
Match: XP_023541655.1 (anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1228 bits (3178), Expect = 0.0
Identity = 612/612 (100.00%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM
Sbjct: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. NCBI nr
Match: KAG7012223.1 (Anaphase-promoting complex subunit 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1222 bits (3162), Expect = 0.0
Identity = 609/612 (99.51%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM
Sbjct: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. NCBI nr
Match: KAG6573034.1 (Anaphase-promoting complex subunit 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1221 bits (3159), Expect = 0.0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQH+KSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHDKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM
Sbjct: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. NCBI nr
Match: XP_022994583.1 (anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQC+ESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM
Sbjct: 481 AQCFESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. NCBI nr
Match: XP_022954782.1 (anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQS+EAAFYYKKDLERM
Sbjct: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSKEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. ExPASy TrEMBL
Match: A0A6J1K5K0 (anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490258 PE=4 SV=1)

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQC+ESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM
Sbjct: 481 AQCFESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. ExPASy TrEMBL
Match: A0A6J1GRV2 (anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456939 PE=4 SV=1)

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 608/612 (99.35%), Postives = 610/612 (99.67%), Query Frame = 0

Query: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60
           MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS
Sbjct: 1   MVGRNEQKAHVSDAHNLKFELQGRALEGRHRSGDSMSSKENCRNELRTAIRQLSDRCLYS 60

Query: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120
           ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE
Sbjct: 61  ASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNEGCSTPITGLSYVSTPVMEE 120

Query: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180
           DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE
Sbjct: 121 DEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKKSVFLRLYALYLAGEKRKEEEVVELE 180

Query: 181 GSLGKSDAVNQELVSLERELSTLRKNVTADPFELYLYGLVLKQKGSENLSRTVLVESVNS 240
           GSLGKSDAVNQELVSLERELSTLRKN TADPFELYLYGLVLKQKGSENL+RTVLVESVNS
Sbjct: 181 GSLGKSDAVNQELVSLERELSTLRKNGTADPFELYLYGLVLKQKGSENLARTVLVESVNS 240

Query: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAGAYQELRMHNESLAKYENLQATF 300
           YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLA AYQELRMHNESLAKYENLQATF
Sbjct: 241 YPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLASAYQELRMHNESLAKYENLQATF 300

Query: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360
           SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL
Sbjct: 301 SFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYL 360

Query: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420
           AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK
Sbjct: 361 AHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMK 420

Query: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480
           NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM
Sbjct: 421 NIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAM 480

Query: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERM 540
           AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQS+EAAFYYKKDLERM
Sbjct: 481 AQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSKEAAFYYKKDLERM 540

Query: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600
           EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS
Sbjct: 541 EAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLAQS 600

Query: 601 SFPSMDVELFPP 612
           SFPSMDVELFPP
Sbjct: 601 SFPSMDVELFPP 612

BLAST of Cp4.1LG09g10460 vs. ExPASy TrEMBL
Match: A0A0A0LW67 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045790 PE=4 SV=1)

HSP 1 Score: 1120 bits (2897), Expect = 0.0
Identity = 554/577 (96.01%), Postives = 566/577 (98.09%), Query Frame = 0

Query: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95
           M+SK+NCR+ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MTSKDNCRHELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 96  RFHTNEGCSTPITGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKK 155
           RFH+NEG STPI G+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREY RAAHVLREQNGKK
Sbjct: 61  RFHSNEGSSTPIAGMSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 156 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELY 215
           SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN   DPF LY
Sbjct: 121 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 216 LYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 275
           LYGLVLKQKGSENL+RT LVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 276 LAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 335
           LA AYQELRMHNESL KYENLQ TFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LASAYQELRMHNESLVKYENLQGTFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 336 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 395
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 396 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 455
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 456 PFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQ 515
           PFYALHYFKK+VFLQPNDSR+WIAMAQCYESEQLRMLE+AIKCYRRAANCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEDAIKCYRRAANCNDREAIALHQ 480

Query: 516 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 575
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAE+YCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEIYCT 540

Query: 576 RLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFPP 612
           RLLDYTGPEKETAK+LLRGMR+AQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKNLLRGMRIAQSSFPSMDVELFPP 577

BLAST of Cp4.1LG09g10460 vs. ExPASy TrEMBL
Match: A0A1S3AVU0 (anaphase-promoting complex subunit 8 OS=Cucumis melo OX=3656 GN=LOC103483216 PE=4 SV=1)

HSP 1 Score: 1119 bits (2894), Expect = 0.0
Identity = 554/577 (96.01%), Postives = 565/577 (97.92%), Query Frame = 0

Query: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95
           M+SK+NCR ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MTSKDNCRYELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 96  RFHTNEGCSTPITGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKK 155
           RFH+NEG STPI G+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREY RAAHVLREQNGKK
Sbjct: 61  RFHSNEGSSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 156 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELY 215
           SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN   DPF LY
Sbjct: 121 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 216 LYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 275
           LYGLVLKQKGSENL+RT LVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 276 LAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 335
           LA AYQELRMHNESL KYENLQ TFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LASAYQELRMHNESLVKYENLQGTFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 336 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 395
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 396 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 455
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 456 PFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQ 515
           PFYALHYFKK+VFLQPNDSR+WIAMAQCYESEQLRMLE+AIKCYRRAANCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEDAIKCYRRAANCNDREAIALHQ 480

Query: 516 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 575
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAE+YCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEIYCT 540

Query: 576 RLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFPP 612
           RLLDYTGPEKETAK+LLRGMR+AQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKNLLRGMRIAQSSFPSMDVELFPP 577

BLAST of Cp4.1LG09g10460 vs. ExPASy TrEMBL
Match: A0A6J1E8K9 (anaphase-promoting complex subunit 8 OS=Cucurbita moschata OX=3662 GN=LOC111431629 PE=4 SV=1)

HSP 1 Score: 1114 bits (2881), Expect = 0.0
Identity = 553/577 (95.84%), Postives = 561/577 (97.23%), Query Frame = 0

Query: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95
           M+SKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 96  RFHTNEGCSTPITGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKK 155
           RFHTNE CSTPI G+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREY RAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 156 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFELY 215
           SVFLR YALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRK    DPF LY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKKGMIDPFGLY 180

Query: 216 LYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 275
           LYGLVLKQKGSENL+RT L+ESVNSYPWNWSAW ELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWLELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 276 LAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 335
           LA AYQELRMHNESL KYENLQATF FSNYIQAQ+AKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYQELRMHNESLVKYENLQATFGFSNYIQAQVAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 336 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 395
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 396 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 455
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 456 PFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQ 515
           PFYALHYFKK+VFLQPNDSR+WIAMAQCYESEQLRMLEEAIKCYRRAA+CNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 516 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 575
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 576 RLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFPP 612
           RLLDYTGPEKETAKDLLRGMRL QSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG09g10460 vs. TAIR 10
Match: AT3G48150.1 (anaphase-promoting complex subunit 8 )

HSP 1 Score: 943.7 bits (2438), Expect = 7.2e-275
Identity = 457/578 (79.07%), Postives = 516/578 (89.27%), Query Frame = 0

Query: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95
           M SKE CRNE+R AIRQLSDRCLYSA+KWA EQLVGIEQDP+ FTP+NTRFQRGSSSIRR
Sbjct: 1   MVSKECCRNEIRAAIRQLSDRCLYSAAKWAGEQLVGIEQDPSNFTPANTRFQRGSSSIRR 60

Query: 96  RFHTNEGCSTPI--TGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNG 155
           RF TNE  STP+   G S  +TP+ EEDE +DGD YLLAKSYFDCREY RA+H+LR+Q  
Sbjct: 61  RFSTNESISTPLPSVGFSQAATPLPEEDEAIDGDIYLLAKSYFDCREYRRASHMLRDQVS 120

Query: 156 KKSVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNVTADPFE 215
           KKS+FLR YALYLAGEKRKEEE++ELEG LGKSDA+N+ELVSLER+LS LR+    D F 
Sbjct: 121 KKSLFLRYYALYLAGEKRKEEEMIELEGPLGKSDAINRELVSLERDLSALRRTGAIDSFG 180

Query: 216 LYLYGLVLKQKGSENLSRTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKD 275
           LYLYG+VLK+KG+E+L+R  LVESVNSYPWNWSAWSELQSLCT+I+ILNSLNLNNHWMK+
Sbjct: 181 LYLYGVVLKEKGNESLARASLVESVNSYPWNWSAWSELQSLCTSIEILNSLNLNNHWMKE 240

Query: 276 FFLAGAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 335
           FFL  AYQELRMH ESLAKYE LQ  FSFSNYIQAQ AKAQYSLREFDQVE +FEELLRN
Sbjct: 241 FFLGNAYQELRMHTESLAKYEYLQGIFSFSNYIQAQTAKAQYSLREFDQVEIMFEELLRN 300

Query: 336 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 395
           DPYRVEDMD+YSNVLYAKE  +ALSYLAH+VF+TDKYRPESCCIIGNYYSLKGQHEK+V+
Sbjct: 301 DPYRVEDMDLYSNVLYAKEACAALSYLAHKVFLTDKYRPESCCIIGNYYSLKGQHEKAVM 360

Query: 396 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 455
           YFRRALKLNK YLSAWTLMGHE+VEMKN PAAIDAYRRAVDIN  DYRAWYGLGQAYEMM
Sbjct: 361 YFRRALKLNKKYLSAWTLMGHEYVEMKNTPAAIDAYRRAVDINPTDYRAWYGLGQAYEMM 420

Query: 456 GMPFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIAL 515
           GMPFYALHYF+K++F  PNDSR+WIAMA+CY++EQL MLEEAIKCY+RA NC D E IAL
Sbjct: 421 GMPFYALHYFRKSIFFLPNDSRLWIAMAKCYQTEQLYMLEEAIKCYKRAVNCTDTEGIAL 480

Query: 516 HQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVY 575
           +QLAKLH +LG++EEAA+Y++KDLERM+AE  EGPNM EAL+FLAT++K  KKF+EAEVY
Sbjct: 481 NQLAKLHQKLGRNEEAAYYFEKDLERMDAEGLEGPNMFEALVFLATHFKNHKKFEEAEVY 540

Query: 576 CTRLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFP 612
           CTRLLDY+GPEKE AK LLRG+R+AQ+ FPSMD+E FP
Sbjct: 541 CTRLLDYSGPEKEKAKSLLRGIRMAQTGFPSMDLEHFP 578

BLAST of Cp4.1LG09g10460 vs. TAIR 10
Match: AT2G20000.1 (CDC27 family protein )

HSP 1 Score: 136.0 bits (341), Expect = 1.0e-31
Identity = 85/301 (28.24%), Postives = 149/301 (49.50%), Query Frame = 0

Query: 288 ESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNV 347
           E+L  Y  L      + ++ +Q+ KA + L ++ + E  F       PY +E MD+YS V
Sbjct: 434 EALDTYMKLPHKHYNTGWVLSQVGKAYFELIDYLEAEKAFRLARLASPYCLEGMDIYSTV 493

Query: 348 LYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLS 407
           LY  +    LSYLA  +  TD+  P+S C +GN YSL+  HE ++  F RA++LN  +  
Sbjct: 494 LYHLKEDMKLSYLAQELISTDRLAPQSWCAMGNCYSLQKDHETALKNFLRAVQLNPRFAY 553

Query: 408 AWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAV 467
           A TL GHE+  +++    + +Y+ A+ +++  Y AWYGLG  Y       ++ H+F+ A 
Sbjct: 554 AHTLCGHEYTTLEDFENGMKSYQNALRVDTRHYNAWYGLGMIYLRQEKLEFSEHHFRMAF 613

Query: 468 FLQPNDSRVWIAMAQCYESEQLRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSE 527
            + P+ S +   +     +  L+  EEA++   +A   + +  + ++Q A +   L + +
Sbjct: 614 LINPSSSVIMSYLGTSLHA--LKRSEEALEIMEQAIVADRKNPLPMYQKANILVCLERLD 673

Query: 528 EAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKET 587
           EA       LE +E  +   P+       +   YK +   D+A ++    LD   P  + 
Sbjct: 674 EA-------LEVLEELKEYAPSESSVYALMGRIYKRRNMHDKAMLHFGLALDMKPPATDV 725

Query: 588 A 589
           A
Sbjct: 734 A 725

BLAST of Cp4.1LG09g10460 vs. TAIR 10
Match: AT3G16320.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 5.2e-31
Identity = 88/319 (27.59%), Postives = 151/319 (47.34%), Query Frame = 0

Query: 276 LAGAYQELRMH--NESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 335
           L   ++ L M+   E+L  Y+ L      ++++  Q+ KA + L+++   ++ F    + 
Sbjct: 391 LGDGHRHLHMYKCQEALLAYQKLSQKQYNTHWVLMQVGKAYFELQDYFNADSSFTLAHQK 450

Query: 336 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 395
            PY +E MD YS VLY  +    L YLA  +   D+  PES C +GN YSL+  H+ ++ 
Sbjct: 451 YPYALEGMDTYSTVLYHLKEEMRLGYLAQELISVDRLSPESWCAVGNCYSLRKDHDTALK 510

Query: 396 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 455
            F+RA++LN+ +  A TL GHEF  ++    A   YR+A+ I++  Y AWYGLG  Y   
Sbjct: 511 MFQRAIQLNERFTYAHTLCGHEFAALEEFEDAERCYRKALGIDTRHYNAWYGLGMTYLRQ 570

Query: 456 GMPFYALHYFKKAVFLQPNDSRVWIAMAQCYES---EQLRMLEEAIKCYRRAANCNDREA 515
               +A H F+ A+ + P  S     +  CY      + +  +EA+    +A   + +  
Sbjct: 571 EKFEFAQHQFQLALQINPRSS-----VIMCYYGIALHESKRNDEALMMMEKAVLTDAKNP 630

Query: 516 IALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEA 575
           +  +  A + + LG        Y K  + +E  +   P        L   Y   K++D+A
Sbjct: 631 LPKYYKAHILTSLGD-------YHKAQKVLEELKECAPQESSVHASLGKIYNQLKQYDKA 690

Query: 576 EVYCTRLLDYTGPEKETAK 590
            ++    LD +    +  K
Sbjct: 691 VLHFGIALDLSPSPSDAVK 697

BLAST of Cp4.1LG09g10460 vs. TAIR 10
Match: AT3G04240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 3.2e-12
Identity = 70/262 (26.72%), Postives = 110/262 (41.98%), Query Frame = 0

Query: 310 IAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAK-ECFSALSYLAHRVFMTD 369
           I    Y L+E+D   A  EE LR  P   E     +N    K +   A+ Y    + +  
Sbjct: 93  IGAIYYQLQEYDMCIARNEEALRIQPQFAECYGNMANAWKEKGDTDRAIRYYLIAIELRP 152

Query: 370 KYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDA 429
            +  ++   + + Y  KG+  ++    ++AL LN   + A + +G+       I  A   
Sbjct: 153 NF-ADAWSNLASAYMRKGRLSEATQCCQQALSLNPLLVDAHSNLGNLMKAQGLIHEAYSC 212

Query: 430 YRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVWIAMAQCYESEQ 489
           Y  AV I      AW  L   +   G    AL Y+K+AV L+P     ++ +   Y++  
Sbjct: 213 YLEAVRIQPTFAIAWSNLAGLFMESGDLNRALQYYKEAVKLKPAFPDAYLNLGNVYKA-- 272

Query: 490 LRMLEEAIKCYRRAANCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGP 549
           L    EAI CY+ A       A+A   +A ++ E GQ + A  +YK+ L R        P
Sbjct: 273 LGRPTEAIMCYQHALQMRPNSAMAFGNIASIYYEQGQLDLAIRHYKQALSR-------DP 332

Query: 550 NMVEALLFLATYYKAQKKFDEA 571
             +EA   L    K   + DEA
Sbjct: 333 RFLEAYNNLGNALKDIGRVDEA 344

BLAST of Cp4.1LG09g10460 vs. TAIR 10
Match: AT5G56290.1 (peroxin 5 )

HSP 1 Score: 59.7 bits (143), Expect = 9.5e-09
Identity = 28/101 (27.72%), Postives = 51/101 (50.50%), Query Frame = 0

Query: 377 IIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDIN 436
           ++G  Y+L  + ++++  F+ AL+L  N  S W  +G           AI AY++A+D+ 
Sbjct: 595 VLGVLYNLSREFDRAITSFQTALQLKPNDYSLWNKLGATQANSVQSADAISAYQQALDLK 654

Query: 437 SCDYRAWYGLGQAYEMMGMPFYALHYFKKAVFLQPNDSRVW 478
               RAW  +G +Y   GM   ++ Y+ +A+ + P     W
Sbjct: 655 PNYVRAWANMGISYANQGMYKESIPYYVRALAMNPKADNAW 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STS31.0e-27379.07Anaphase-promoting complex subunit 8 OS=Arabidopsis thaliana OX=3702 GN=APC8 PE=... [more]
Q86B113.4e-12843.72Anaphase-promoting complex subunit 8 OS=Dictyostelium discoideum OX=44689 GN=ana... [more]
A1A4R82.4e-11848.23Cell division cycle protein 23 homolog OS=Bos taurus OX=9913 GN=CDC23 PE=2 SV=1[more]
Q9UJX22.4e-11848.23Cell division cycle protein 23 homolog OS=Homo sapiens OX=9606 GN=CDC23 PE=1 SV=... [more]
Q8BGZ41.6e-11748.02Cell division cycle protein 23 homolog OS=Mus musculus OX=10090 GN=Cdc23 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_023541655.10.0100.00anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
KAG7012223.10.099.51Anaphase-promoting complex subunit 8 [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6573034.10.099.35Anaphase-promoting complex subunit 8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022994583.10.099.35anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita maxima][more]
XP_022954782.10.099.35anaphase-promoting complex subunit 8-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1K5K00.099.35anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1GRV20.099.35anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A0A0LW670.096.01TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045790 ... [more]
A0A1S3AVU00.096.01anaphase-promoting complex subunit 8 OS=Cucumis melo OX=3656 GN=LOC103483216 PE=... [more]
A0A6J1E8K90.095.84anaphase-promoting complex subunit 8 OS=Cucurbita moschata OX=3662 GN=LOC1114316... [more]
Match NameE-valueIdentityDescription
AT3G48150.17.2e-27579.07anaphase-promoting complex subunit 8 [more]
AT2G20000.11.0e-3128.24CDC27 family protein [more]
AT3G16320.15.2e-3127.59Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G04240.13.2e-1226.72Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G56290.19.5e-0927.72peroxin 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 186..206
NoneNo IPR availablePFAMPF13414TPR_11coord: 413..454
e-value: 7.8E-8
score: 31.9
NoneNo IPR availablePANTHERPTHR12558:SF37BNAA06G16860D PROTEINcoord: 39..611
NoneNo IPR availablePANTHERPTHR12558CELL DIVISION CYCLE 16,23,27coord: 39..611
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 406..439
e-value: 1.5
score: 17.8
coord: 440..473
e-value: 8.0E-5
score: 32.0
coord: 304..337
e-value: 230.0
score: 3.0
coord: 474..509
e-value: 24.0
score: 11.6
coord: 372..405
e-value: 7.6E-5
score: 32.1
coord: 510..543
e-value: 11.0
score: 14.5
coord: 551..584
e-value: 2.8
score: 16.9
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 373..404
e-value: 3.7E-5
score: 23.6
IPR019734Tetratricopeptide repeatPFAMPF13176TPR_7coord: 512..541
e-value: 0.0035
score: 17.3
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 406..439
score: 9.4699
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 440..473
score: 10.5024
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 372..405
score: 8.8504
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 36..368
e-value: 3.0E-103
score: 347.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 369..579
e-value: 2.7E-57
score: 195.6
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 372..538
IPR007192Cdc23PFAMPF04049ANAPC8coord: 42..192
e-value: 3.4E-42
score: 143.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g10460.1Cp4.1LG09g10460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0051301 cell division
biological_process GO:0045842 positive regulation of mitotic metaphase/anaphase transition
biological_process GO:0016567 protein ubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005680 anaphase-promoting complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding