Cp4.1LG08g13470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g13470
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin-protein ligase
LocationCp4.1LG08: 9748419 .. 9751932 (+)
RNA-Seq ExpressionCp4.1LG08g13470
SyntenyCp4.1LG08g13470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCTGTAGCTCACCAGTTGCCACCCGCCTCAGCTCCCACATTCTCTTTCTCTCTCTACACAACTACTTCACCAGCACCCATTCTCTCTCTCTCTCTCCCGAATTCTCTCTCAACTGGAGGCGGAGGTCATAATGGAAGAAGAGCGAGCACTCAGAAGCAGCGCCAATGGCGGTTCTTCTGTTCTTCGCTACCCCACTGTCAGCACCACTTTTACAATCCCTTTAACTCGCTACACTACCCGTTTGATTACCTCCGACCGCCACCGCGTCCTTTTGGCTGATTGCGCCGACCCTCGACCGGACGCTGAAGAAGATGATGATGACGAAGACGGCTCCAACGGATTCAATCACCGCTCCTTCTCCTACTCTATGCCCATTTTGGTTCTCGACGTTCTCTGGAATTTGGCCTTTGTTCTTGTCTCCATTGTTGTCCTCTTGTCCACGTTTCGTGAGCGCCCTTCCACCCCTTTGCGCCTTTGGATTTCTGGGTATGCCTTCCAATGTCTTCTGCACATTGGGTTTGTCTTCTTTGAGTACCAGAGGAGTATCCTGCATCATGGCTTTGAGGACCCTGCGGCTCATCGCAGGTAGGGAAAACAGTCTATTATGATTTCATTTCAGTGTTAGGGGAGGTTTTGTGCTCTTTGTTGGCTGTGGCTGTCTACATTGCTGACCTTTTTCCAATCCCCCCTGAATTTTGAGCTTGAAATTTGAAGATGGAGCCACTAATTTTTGCTTGTATGGTTTTTTGATGTGATGAGGGGATGGATAATTGAGCATGCATGTTCTAAGAAATAGATGGAAAGGGGAAAATGTAAATTCTTTATTCGGTGTTTATTTTCTTGATTTCATGTTTCATTCAACCTGTAATTAACTTCGCATCATCTTCTCAAATTTAGTCTTCAACTTACTTGACGAACACATAACAATATACAGATCTATAAGCTGTACCAGCAAGCTTTGGACTAATTTGTTTTATCTTTGTCCTTCTAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACATCGTCGGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCACGCACTTCTCCAAGATTCTCCCCGGCTTTATTGGTAAGGACTTTGCCTTTTGACATGTTTTCTGATGGAGAAATGGTGTCACTGGCAAATTTCATCTTCTTTGAAAGTTGTTTCAACTGCTATCTTTTGGTTGGTTTTGCCTTTGAATTAACTGAATGTGTTCACACTCTTCAATCTGGACTTTACTTTTTTCTTTTTAGCGAACTTTCAACAATGTCCTACAGAAACTGGTACTTTAAGTCATGGCTTTTCTAAATTAGGTTTCTTGTAATAATTGCATATCAGACTAAGCTGATGAGAGTTCGATCTCAAACAATTATACAAATGGCTATGCAAAACTTAAGGTATATCTTTAATATTCTGGTTCCAATCTCGATCATTCGGTTTTTTGTGGGTCGGTGGGTGGGTTCTAGATCCTTTTATTCTCCTCTCTCGTATGTGAGAGATATCTTACAAGACCGGTGTCAATTCGAAGAACGTTCTTGTTTTGCTACAAAAAGAACAGTGAAAAGGTACCAATGGTCCCTTGAGAAGTTGATAATGTTTCCAATACAATAATTGAACCTAAATCCTGTAAATAATAACCAGTGGATAGCAAGACAAGGAAAACTAAGTAAATGCATTAAATTTTGTTACATCTGCTTTCTTGATCCAATCTGATACTTTGTCAACAAATGTATTTGAATTTCTTTCAAACCGTAATTTTGAAACTTTTAGCTTTAATTGTGTTCACCTAAAGAAATCTTAGCTTTGATTTATTGTTGTTCGGATCATTTAGCCTCATTTGGAGCTTTGATGCTGAATATTTTTTGGGTCAATTGCAGGTTAACCGTGGTCTTTCTAGCATTTGACTTGTTCTTTATTCTCTTTTGTGTTGGGATGGCATTTGTTGTTTGCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACCAGAGAAGGCGCCTCAGATGAGGATATCAGGACTCTTCCCAAGTATACATTTCGTCAAGCTCCGGTATTTGGGACATTTAACAAAGAACAAAAACCTGTCGGAGCAAGATTGGAGTTGGATAATGATCACCACATCAAAGAACTTGTTCTTCATCCAGAGGATTCTGTCAGCTACTTATCTTTTATTTCAAATCGTTAATATTTATATTCTATCTGGTTGATTGTTTATGATTCTATTACATCATTATTATCTCCTGCAAAAAGTCCCGGAAATTGCACGATTCAGATCTGCATGTGCATTACATATTATGTGCATGCAGTAGTTGATGATCATTCTAAAATTTTGTGCGTATTTTGATTCTGTTCTCAGCTAGAATGAAGAGTATGTATTCCTTCTTGAACCATGAAAGATCCATGGAACTTTAAAAAAGGCCAATCTGGGTTCAATAATTGGGAATCTTGGTTATAATAACTCAATTTCTTGACTGCCTGCATAGATGGAAACAGAAATATCCTTTTTGTAACATCCCAAGCTCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCCTTTGGGCGTCCTCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGATTTTCACACCCTTGTAAAGAATGCTTCGTTCTCCTCTCCAACCGATATGGGATTTGACAATCCACCCCCCTTTGGGTCCCAACAACCTCGAACCCTTATAAAAAATGCTTCGTTCTCCTCCCGAACCAACATGGGATGTCAAACTTTTCTGGCTTATAGTAATGAGTAAAAATGTATATCAATATGGTTCTCTGTGTGCACAATAGTTAGTGATTCTATGACAGGATGTACTTTCTTCATGGTGTGCAAATTTTTGTTTCGTTTCTCTCACTGGAATGTTCATCCTGTCATTGCTTTTGAATCATGGTAGTCTTTTTCTTGTTGCATTTATGCTGTTTAGTTGAGAGTTTTCCCTAATTTGAAGCTGAGTATTCAAATATTTACTTTTGTGTAGGAATGTTGCATTTGCCTTTCACAATATGAAGACGGGACGGAGCTCTATACTCTTCCCTGCAACCACCATTTCCATTGTGGGTGCATTGGCAAATGGCTTCGGATAAAGGCAACCTGCCCGCTCTGCAAGTCTAATATCCATTGGGGCGATACATTGGTTTAACTATCGGAACGAGCAACAAATCCAGCTGAGAATTTGAAAGTTAGGATTTTCAATTTCAGGTCAGATGCTTGTTGATCTCTGTCAACTAATGCTTTTAGAAACAGGGAGGGTGGAGAGTAGGAATTTAGCTGATTCCAACCTTAGGGAGATGTTTAGTTTCCTTAAACCCCATCTGTGGACACAATTTAATCTGAAAATTTAACTTTATTTGTATGTAAATTGATAGGAAAAGGTCCTTGTGCCTCTAATTTAATCTGCTATATGTGAATTCTTCTGTGTCAGTTTACTGGCTACTCTGTTTTCAAGCCTCT

mRNA sequence

CGCTGTAGCTCACCAGTTGCCACCCGCCTCAGCTCCCACATTCTCTTTCTCTCTCTACACAACTACTTCACCAGCACCCATTCTCTCTCTCTCTCTCCCGAATTCTCTCTCAACTGGAGGCGGAGGTCATAATGGAAGAAGAGCGAGCACTCAGAAGCAGCGCCAATGGCGGTTCTTCTGTTCTTCGCTACCCCACTGTCAGCACCACTTTTACAATCCCTTTAACTCGCTACACTACCCGTTTGATTACCTCCGACCGCCACCGCGTCCTTTTGGCTGATTGCGCCGACCCTCGACCGGACGCTGAAGAAGATGATGATGACGAAGACGGCTCCAACGGATTCAATCACCGCTCCTTCTCCTACTCTATGCCCATTTTGGTTCTCGACGTTCTCTGGAATTTGGCCTTTGTTCTTGTCTCCATTGTTGTCCTCTTGTCCACGTTTCGTGAGCGCCCTTCCACCCCTTTGCGCCTTTGGATTTCTGGGTATGCCTTCCAATGTCTTCTGCACATTGGGTTTGTCTTCTTTGAGTACCAGAGGAGTATCCTGCATCATGGCTTTGAGGACCCTGCGGCTCATCGCAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACATCGTCGGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCACGCACTTCTCCAAGATTCTCCCCGGCTTTATTGGTTAACCGTGGTCTTTCTAGCATTTGACTTGTTCTTTATTCTCTTTTGTGTTGGGATGGCATTTGTTGTTTGCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACCAGAGAAGGCGCCTCAGATGAGGATATCAGGACTCTTCCCAAGTATACATTTCGTCAAGCTCCGGTATTTGGGACATTTAACAAAGAACAAAAACCTGTCGGAGCAAGATTGGAGTTGGATAATGATCACCACATCAAAGAACTTGTTCTTCATCCAGAGGATTCTGAATGTTGCATTTGCCTTTCACAATATGAAGACGGGACGGAGCTCTATACTCTTCCCTGCAACCACCATTTCCATTGTGGGTGCATTGGCAAATGGCTTCGGATAAAGGCAACCTGCCCGCTCTGCAAGTCTAATATCCATTGGGGCGATACATTGGTTTAACTATCGGAACGAGCAACAAATCCAGCTGAGAATTTGAAAGTTAGGATTTTCAATTTCAGGTCAGATGCTTGTTGATCTCTGTCAACTAATGCTTTTAGAAACAGGGAGGGTGGAGAGTAGGAATTTAGCTGATTCCAACCTTAGGGAGATGTTTAGTTTCCTTAAACCCCATCTGTGGACACAATTTAATCTGAAAATTTAACTTTATTTGTATGTAAATTGATAGGAAAAGGTCCTTGTGCCTCTAATTTAATCTGCTATATGTGAATTCTTCTGTGTCAGTTTACTGGCTACTCTGTTTTCAAGCCTCT

Coding sequence (CDS)

ATGGAAGAAGAGCGAGCACTCAGAAGCAGCGCCAATGGCGGTTCTTCTGTTCTTCGCTACCCCACTGTCAGCACCACTTTTACAATCCCTTTAACTCGCTACACTACCCGTTTGATTACCTCCGACCGCCACCGCGTCCTTTTGGCTGATTGCGCCGACCCTCGACCGGACGCTGAAGAAGATGATGATGACGAAGACGGCTCCAACGGATTCAATCACCGCTCCTTCTCCTACTCTATGCCCATTTTGGTTCTCGACGTTCTCTGGAATTTGGCCTTTGTTCTTGTCTCCATTGTTGTCCTCTTGTCCACGTTTCGTGAGCGCCCTTCCACCCCTTTGCGCCTTTGGATTTCTGGGTATGCCTTCCAATGTCTTCTGCACATTGGGTTTGTCTTCTTTGAGTACCAGAGGAGTATCCTGCATCATGGCTTTGAGGACCCTGCGGCTCATCGCAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACATCGTCGGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCACGCACTTCTCCAAGATTCTCCCCGGCTTTATTGGTTAACCGTGGTCTTTCTAGCATTTGACTTGTTCTTTATTCTCTTTTGTGTTGGGATGGCATTTGTTGTTTGCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACCAGAGAAGGCGCCTCAGATGAGGATATCAGGACTCTTCCCAAGTATACATTTCGTCAAGCTCCGGTATTTGGGACATTTAACAAAGAACAAAAACCTGTCGGAGCAAGATTGGAGTTGGATAATGATCACCACATCAAAGAACTTGTTCTTCATCCAGAGGATTCTGAATGTTGCATTTGCCTTTCACAATATGAAGACGGGACGGAGCTCTATACTCTTCCCTGCAACCACCATTTCCATTGTGGGTGCATTGGCAAATGGCTTCGGATAAAGGCAACCTGCCCGCTCTGCAAGTCTAATATCCATTGGGGCGATACATTGGTTTAA

Protein sequence

MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEEDDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASDEDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Homology
BLAST of Cp4.1LG08g13470 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.4e-68
Identity = 136/308 (44.16%), Postives = 180/308 (58.44%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++VLD++WNLAFV V+  +L+ + +E P  PLR+W+ GYA QC+LH+  V  EY
Sbjct: 94  WAYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEY 153

Query: 136 QR-----------------------------------SILHHGFED------PAAHRSIM 195
           +R                                   S  + G +D           S+ 
Sbjct: 154 RRRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVA 213

Query: 196 KRLESMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFV 255
           K LES NTM S +WW+ GFYW+  GG  L Q+SPR+YWL++VFL FD+FF++FCV +A V
Sbjct: 214 KHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACV 273

Query: 256 VCFSLCCCIP-IVAFAYAMTTREGASDEDIRTLPKYTFRQAPVFGTFNK------EQKPV 315
           +  ++CCC+P I+A  YA+  +EGAS EDI  L K+ FR+    G  NK      +    
Sbjct: 274 IGIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTE 333

Query: 316 GARLELDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKAT 336
           G   E   D  I+  +L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL I AT
Sbjct: 334 GIMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINAT 393

BLAST of Cp4.1LG08g13470 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.4e-68
Identity = 130/284 (45.77%), Postives = 170/284 (59.86%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++ LD+LWNLAFV + + VL+ +  E+P+ PLR+W+ GY  QC LH+  V  EY
Sbjct: 103 WAYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEY 162

Query: 136 QRS----------------------ILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGF 195
           +R                       +     ED     +  K LES NTM S +WW+ GF
Sbjct: 163 RRRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGF 222

Query: 196 YWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIP-IVAFAYAM 255
           YW+  GG  L  DSP+LYWL ++FL FD+FF++FCV +A V+  ++CCC+P I+A  YA+
Sbjct: 223 YWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAV 282

Query: 256 TTREGASDEDIRTLPKYTFRQAPVFGTFNK-EQKPVGARLELDNDHHIKELVLHPEDSEC 315
             +EGAS  DI  +PK+ F +    G   K   K  G   E   D  I E  L PED+EC
Sbjct: 283 ADQEGASKNDIDQMPKFRFTKT---GNVEKLSGKARGIMTECGTDSPI-ERSLSPEDAEC 342

Query: 316 CICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNI 336
           CICL +YEDG EL  LPCNHHFHC CI KWL I + CPLCK NI
Sbjct: 343 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Cp4.1LG08g13470 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 134/303 (44.22%), Postives = 177/303 (58.42%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++VLD +WNLAFV+V+  VL+ +  E P+ PLR+WI GY  QC++H+  V  EY
Sbjct: 68  WAYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEY 127

Query: 136 QR-----------------SILHHGFEDPAA--------------------HRSIMKRLE 195
           +R                 S      ++                       + S  K LE
Sbjct: 128 RRRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLE 187

Query: 196 SMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFS 255
           S NTM S +WWV GFYW+  GG  L Q SP+LYWL +VFL FD+FF++FCV +A V+  +
Sbjct: 188 SANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIA 247

Query: 256 LCCCIP-IVAFAYAMTTREGASDEDIRTLPKYTFRQA--PVFGTFNKEQ---KPVGARLE 315
           +CCC+P I+A  YA+  +EGAS EDI  L K+ FR+    +  T ++EQ      G   E
Sbjct: 248 VCCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTE 307

Query: 316 LDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCK 336
              D  ++  + H ED+ECCICLS YED TEL  LPC HHFHCGC+ KWL I ATCPLCK
Sbjct: 308 CGTDSPVEHALPH-EDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCK 367

BLAST of Cp4.1LG08g13470 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.7e-65
Identity = 127/292 (43.49%), Postives = 175/292 (59.93%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           + YS P++  D+LWN A VL S V+L+ T  ERP+ P+R+WI  Y  QCL H+  V+ EY
Sbjct: 71  WGYSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEY 130

Query: 136 QR-----------SILHHGF----------EDPAAHRSIMKRLESMNTMTSSVWWVFGFY 195
            R           S  H  +          +D +   S +KR ES+NT+ S +WW+ GFY
Sbjct: 131 WRRNSTRRARDLESYDHEDYNIEYDYEQDSDDNSTTYSFVKRCESINTVISFIWWIIGFY 190

Query: 196 WIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIP-IVAFAYAMT 255
           W+V GG  LL ++P LYWL+V+FLA D+FF +FCV +A +V  +LCCC+P I+A  YA+ 
Sbjct: 191 WVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIALLYAVA 250

Query: 256 TREGASDEDIRTLPKYTFRQAPVFGTFNKEQKPVG-----ARLELDNDHHIKELVLHPED 315
             EG S+ ++  LP Y F+       F+  +K +        + ++      E  L  ED
Sbjct: 251 GTEGVSEAELGVLPLYKFK------AFHSNEKNITGPGKMVPIPINGLCLATERTLLAED 310

Query: 316 SECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDT 341
           ++CCICLS YEDG EL+ LPCNHHFH  CI KWL+++ATCPLCK NI  G T
Sbjct: 311 ADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILKGTT 356

BLAST of Cp4.1LG08g13470 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 4.2e-11
Identity = 25/47 (53.19%), Postives = 33/47 (70.21%), Query Frame = 0

Query: 287 EDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKS 334
           E+  CCIC  +Y DG +L TL C H FH GC+ +WL +K TCP+CK+
Sbjct: 616 EEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKN 662

BLAST of Cp4.1LG08g13470 vs. NCBI nr
Match: XP_023539643.1 (E3 ubiquitin-protein ligase At4g11680-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708 bits (1827), Expect = 1.18e-257
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE
Sbjct: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. NCBI nr
Match: KAG6596184.1 (E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702 bits (1813), Expect = 1.60e-255
Identity = 338/342 (98.83%), Postives = 341/342 (99.71%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALR SANGGSSVLRYPTVSTTFT+PLTRYTTRLITSDRHR+LLADCADPRPDAEE
Sbjct: 1   MEEERALRRSANGGSSVLRYPTVSTTFTMPLTRYTTRLITSDRHRILLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGS+GFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSSGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. NCBI nr
Match: KAG7027721.1 (E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702 bits (1813), Expect = 1.60e-255
Identity = 338/342 (98.83%), Postives = 341/342 (99.71%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           M+EERALR SANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHR+LLADCADPRPDAEE
Sbjct: 1   MDEERALRRSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRILLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGS+GFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSSGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. NCBI nr
Match: XP_022937857.1 (E3 ubiquitin-protein ligase At4g11680-like [Cucurbita moschata])

HSP 1 Score: 700 bits (1806), Expect = 1.87e-254
Identity = 338/342 (98.83%), Postives = 340/342 (99.42%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALR SANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE
Sbjct: 1   MEEERALRRSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGS+GFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSSGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQ+SILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQKSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPV ARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVRARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. NCBI nr
Match: XP_022971451.1 (E3 ubiquitin-protein ligase At4g11680-like [Cucurbita maxima])

HSP 1 Score: 699 bits (1805), Expect = 2.66e-254
Identity = 339/342 (99.12%), Postives = 339/342 (99.12%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALR SANGGSSVLR PTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE
Sbjct: 1   MEEERALRRSANGGSSVLRXPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQRSILHHGFED AAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQRSILHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. ExPASy TrEMBL
Match: A0A6J1FCD6 (E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita moschata OX=3662 GN=LOC111444117 PE=4 SV=1)

HSP 1 Score: 700 bits (1806), Expect = 9.06e-255
Identity = 338/342 (98.83%), Postives = 340/342 (99.42%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALR SANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE
Sbjct: 1   MEEERALRRSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGS+GFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSSGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQ+SILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQKSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPV ARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVRARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. ExPASy TrEMBL
Match: A0A6J1I3D4 (E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita maxima OX=3661 GN=LOC111470167 PE=4 SV=1)

HSP 1 Score: 699 bits (1805), Expect = 1.29e-254
Identity = 339/342 (99.12%), Postives = 339/342 (99.12%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEEERALR SANGGSSVLR PTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE
Sbjct: 1   MEEERALRRSANGGSSVLRXPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60

Query: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120
           DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY
Sbjct: 61  DDDDEDGSNGFNHRSFSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGY 120

Query: 121 AFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           AFQCLLHIGFVFFEYQRSILHHGFED AAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 AFQCLLHIGFVFFEYQRSILHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240
           HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD
Sbjct: 181 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTREGASD 240

Query: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300
           EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED
Sbjct: 241 EDIRTLPKYTFRQAPVFGTFNKEQKPVGARLELDNDHHIKELVLHPEDSECCICLSQYED 300

Query: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV
Sbjct: 301 GTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342

BLAST of Cp4.1LG08g13470 vs. ExPASy TrEMBL
Match: A0A6J1J3G3 (E3 ubiquitin-protein ligase At4g11680-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482323 PE=4 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 1.66e-201
Identity = 288/352 (81.82%), Postives = 304/352 (86.36%), Query Frame = 0

Query: 1   MEEERALRSSANGGS---SVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPD 60
           MEEERALR +ANGG+   SVLRYPTVSTTFTIPLTRYTTRLITS RHR LLADCAD R D
Sbjct: 1   MEEERALRRTANGGTLNDSVLRYPTVSTTFTIPLTRYTTRLITSGRHRPLLADCADSRSD 60

Query: 61  AEEDDDDEDGSNGFNHRSFS-----YSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTP 120
            E+ DD    +N FN RS       YSMPIL+LDV+WNLAFV VSIVVLLSTFRERPSTP
Sbjct: 61  TEDHDD----ANDFNRRSIVRENCFYSMPILILDVVWNLAFVFVSIVVLLSTFRERPSTP 120

Query: 121 LRLWISGYAFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFG 180
           LRLWISGY  QCLLH+GFVFFEYQRS+  HGFED A HRSIMKRLESMNTMTSSVWWVFG
Sbjct: 121 LRLWISGYGLQCLLHVGFVFFEYQRSMALHGFEDRAYHRSIMKRLESMNTMTSSVWWVFG 180

Query: 181 FYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAM 240
           FYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFC+GMA VV FSLCCCIPI+AFAYAM
Sbjct: 181 FYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVVFFSLCCCIPIIAFAYAM 240

Query: 241 TTREGASDEDIRTLPKYTFRQAPVFGTF--NKEQKPVGARLELDNDHHIKELVLHPEDSE 300
           T REGAS+E IR LPKYTFRQA + G F   KE++P+GA  ELDNDH IKEL LHPEDSE
Sbjct: 241 TAREGASEEVIRALPKYTFRQAAL-GAFIHGKEREPIGATTELDNDHLIKELALHPEDSE 300

Query: 301 CCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           CCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRI ATCPLCKS+I  GDTLV
Sbjct: 301 CCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRINATCPLCKSSICRGDTLV 347

BLAST of Cp4.1LG08g13470 vs. ExPASy TrEMBL
Match: A0A5D3DQ81 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006360 PE=4 SV=1)

HSP 1 Score: 564 bits (1454), Expect = 3.30e-201
Identity = 282/349 (80.80%), Postives = 302/349 (86.53%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEE+RALR + N   S+LRYPTVSTTFTIPLTRYTTRLI+SDRHR LLADCA+   D   
Sbjct: 1   MEEQRALRPTLN--HSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD--- 60

Query: 61  DDDDEDGSNGFNHRSF-----SYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRL 120
                  S+ F+ RSF     SYSMPILVLDV+WNLAFVLVS+VVL STFRERPSTPLRL
Sbjct: 61  -------SDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRL 120

Query: 121 WISGYAFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYW 180
           WISGY  QCLLH+GFVFFEYQRS+ HHGFED AAHRSIMKRLESMNTMTSSVWWVFGFYW
Sbjct: 121 WISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYW 180

Query: 181 IVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTR 240
           IVMGG ALLQDSPRLYWLTVVFLAFDLFFILFC+GMA V+ FSLCCCIPIVAFAYAMTTR
Sbjct: 181 IVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTR 240

Query: 241 EGASDEDIRTLPKYTFRQAPVFGTFN--KEQKPVGARLELDNDHHIKELVLHPEDSECCI 300
           EGAS+EDIRTLPKYTFRQA V GTFN  KE++P+ + +ELDN H IKEL LHPEDSECCI
Sbjct: 241 EGASEEDIRTLPKYTFRQAAVLGTFNLGKEREPISSTVELDNGHRIKELALHPEDSECCI 300

Query: 301 CLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           CLS+YEDGTELYTLPCNHHFHCGCI KWLRI ATCPLCKSNI  GDTLV
Sbjct: 301 CLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337

BLAST of Cp4.1LG08g13470 vs. ExPASy TrEMBL
Match: A0A1S3B880 (E3 ubiquitin-protein ligase At4g11680-like OS=Cucumis melo OX=3656 GN=LOC103486858 PE=4 SV=1)

HSP 1 Score: 564 bits (1454), Expect = 3.30e-201
Identity = 282/349 (80.80%), Postives = 302/349 (86.53%), Query Frame = 0

Query: 1   MEEERALRSSANGGSSVLRYPTVSTTFTIPLTRYTTRLITSDRHRVLLADCADPRPDAEE 60
           MEE+RALR + N   S+LRYPTVSTTFTIPLTRYTTRLI+SDRHR LLADCA+   D   
Sbjct: 1   MEEQRALRPTLN--HSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD--- 60

Query: 61  DDDDEDGSNGFNHRSF-----SYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRL 120
                  S+ F+ RSF     SYSMPILVLDV+WNLAFVLVS+VVL STFRERPSTPLRL
Sbjct: 61  -------SDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRL 120

Query: 121 WISGYAFQCLLHIGFVFFEYQRSILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGFYW 180
           WISGY  QCLLH+GFVFFEYQRS+ HHGFED AAHRSIMKRLESMNTMTSSVWWVFGFYW
Sbjct: 121 WISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYW 180

Query: 181 IVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIPIVAFAYAMTTR 240
           IVMGG ALLQDSPRLYWLTVVFLAFDLFFILFC+GMA V+ FSLCCCIPIVAFAYAMTTR
Sbjct: 181 IVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTR 240

Query: 241 EGASDEDIRTLPKYTFRQAPVFGTFN--KEQKPVGARLELDNDHHIKELVLHPEDSECCI 300
           EGAS+EDIRTLPKYTFRQA V GTFN  KE++P+ + +ELDN H IKEL LHPEDSECCI
Sbjct: 241 EGASEEDIRTLPKYTFRQAAVLGTFNLGKEREPISSTVELDNGHRIKELALHPEDSECCI 300

Query: 301 CLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGDTLV 342
           CLS+YEDGTELYTLPCNHHFHCGCI KWLRI ATCPLCKSNI  GDTLV
Sbjct: 301 CLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337

BLAST of Cp4.1LG08g13470 vs. TAIR 10
Match: AT1G68070.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.4e-71
Identity = 136/285 (47.72%), Postives = 184/285 (64.56%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           + YS P++ LD+LWN AFVLV+IV+LL    E+P+ P+R+WI GYA QCL+H+  V+ E+
Sbjct: 62  WGYSKPVVALDMLWNTAFVLVAIVMLLVFKEEKPNVPIRIWICGYAIQCLVHVVLVWLEF 121

Query: 136 QR-------------SILHHGFEDPAAHRSIM--KRLESMNTMTSSVWWVFGFYWIVMGG 195
           ++                +   ED       +  K  ESMNT+ S VWW+ GFYW+V GG
Sbjct: 122 RKRNARSRPGDLEAAQATNQDSEDEDNDERFLSTKTCESMNTIISFVWWIVGFYWLVSGG 181

Query: 196 HALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIP-IVAFAYAMTTREGAS 255
             LLQ++  LYWLT VFLAFD+FF +FCV +A ++  +LCCC+P I+A  YA+  +EGAS
Sbjct: 182 DILLQNATHLYWLTFVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIALLYAVAGQEGAS 241

Query: 256 DEDIRTLPKYTFRQAPVFGTFNKEQKPV---GARLELD--NDHHIKELVLHPEDSECCIC 315
           + D+  LPKY F       T N ++K     G  + +D  +++   E VL PED++CCIC
Sbjct: 242 EADLSILPKYRFH------TMNNDEKQSDGGGKMIPVDAASENLGNERVLLPEDADCCIC 301

Query: 316 LSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNIHWGD 340
           LS YEDG EL +LPCNHHFH  CI KWL++ ATCPLCK NI  G+
Sbjct: 302 LSSYEDGAELVSLPCNHHFHSTCIVKWLKMNATCPLCKFNILKGN 340

BLAST of Cp4.1LG08g13470 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.0e-69
Identity = 136/308 (44.16%), Postives = 180/308 (58.44%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++VLD++WNLAFV V+  +L+ + +E P  PLR+W+ GYA QC+LH+  V  EY
Sbjct: 94  WAYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEY 153

Query: 136 QR-----------------------------------SILHHGFED------PAAHRSIM 195
           +R                                   S  + G +D           S+ 
Sbjct: 154 RRRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVA 213

Query: 196 KRLESMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFV 255
           K LES NTM S +WW+ GFYW+  GG  L Q+SPR+YWL++VFL FD+FF++FCV +A V
Sbjct: 214 KHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACV 273

Query: 256 VCFSLCCCIP-IVAFAYAMTTREGASDEDIRTLPKYTFRQAPVFGTFNK------EQKPV 315
           +  ++CCC+P I+A  YA+  +EGAS EDI  L K+ FR+    G  NK      +    
Sbjct: 274 IGIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTE 333

Query: 316 GARLELDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKAT 336
           G   E   D  I+  +L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL I AT
Sbjct: 334 GIMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINAT 393

BLAST of Cp4.1LG08g13470 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.0e-69
Identity = 136/308 (44.16%), Postives = 180/308 (58.44%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++VLD++WNLAFV V+  +L+ + +E P  PLR+W+ GYA QC+LH+  V  EY
Sbjct: 23  WAYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEY 82

Query: 136 QR-----------------------------------SILHHGFED------PAAHRSIM 195
           +R                                   S  + G +D           S+ 
Sbjct: 83  RRRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVA 142

Query: 196 KRLESMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFV 255
           K LES NTM S +WW+ GFYW+  GG  L Q+SPR+YWL++VFL FD+FF++FCV +A V
Sbjct: 143 KHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACV 202

Query: 256 VCFSLCCCIP-IVAFAYAMTTREGASDEDIRTLPKYTFRQAPVFGTFNK------EQKPV 315
           +  ++CCC+P I+A  YA+  +EGAS EDI  L K+ FR+    G  NK      +    
Sbjct: 203 IGIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTE 262

Query: 316 GARLELDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKAT 336
           G   E   D  I+  +L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL I AT
Sbjct: 263 GIMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINAT 322

BLAST of Cp4.1LG08g13470 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.0e-69
Identity = 130/284 (45.77%), Postives = 170/284 (59.86%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++ LD+LWNLAFV + + VL+ +  E+P+ PLR+W+ GY  QC LH+  V  EY
Sbjct: 103 WAYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEY 162

Query: 136 QRS----------------------ILHHGFEDPAAHRSIMKRLESMNTMTSSVWWVFGF 195
           +R                       +     ED     +  K LES NTM S +WW+ GF
Sbjct: 163 RRRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGF 222

Query: 196 YWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFSLCCCIP-IVAFAYAM 255
           YW+  GG  L  DSP+LYWL ++FL FD+FF++FCV +A V+  ++CCC+P I+A  YA+
Sbjct: 223 YWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAV 282

Query: 256 TTREGASDEDIRTLPKYTFRQAPVFGTFNK-EQKPVGARLELDNDHHIKELVLHPEDSEC 315
             +EGAS  DI  +PK+ F +    G   K   K  G   E   D  I E  L PED+EC
Sbjct: 283 ADQEGASKNDIDQMPKFRFTKT---GNVEKLSGKARGIMTECGTDSPI-ERSLSPEDAEC 342

Query: 316 CICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCKSNI 336
           CICL +YEDG EL  LPCNHHFHC CI KWL I + CPLCK NI
Sbjct: 343 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Cp4.1LG08g13470 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.3e-67
Identity = 134/303 (44.22%), Postives = 177/303 (58.42%), Query Frame = 0

Query: 76  FSYSMPILVLDVLWNLAFVLVSIVVLLSTFRERPSTPLRLWISGYAFQCLLHIGFVFFEY 135
           ++YS P++VLD +WNLAFV+V+  VL+ +  E P+ PLR+WI GY  QC++H+  V  EY
Sbjct: 68  WAYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEY 127

Query: 136 QR-----------------SILHHGFEDPAA--------------------HRSIMKRLE 195
           +R                 S      ++                       + S  K LE
Sbjct: 128 RRRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLE 187

Query: 196 SMNTMTSSVWWVFGFYWIVMGGHALLQDSPRLYWLTVVFLAFDLFFILFCVGMAFVVCFS 255
           S NTM S +WWV GFYW+  GG  L Q SP+LYWL +VFL FD+FF++FCV +A V+  +
Sbjct: 188 SANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIA 247

Query: 256 LCCCIP-IVAFAYAMTTREGASDEDIRTLPKYTFRQA--PVFGTFNKEQ---KPVGARLE 315
           +CCC+P I+A  YA+  +EGAS EDI  L K+ FR+    +  T ++EQ      G   E
Sbjct: 248 VCCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTE 307

Query: 316 LDNDHHIKELVLHPEDSECCICLSQYEDGTELYTLPCNHHFHCGCIGKWLRIKATCPLCK 336
              D  ++  + H ED+ECCICLS YED TEL  LPC HHFHCGC+ KWL I ATCPLCK
Sbjct: 308 CGTDSPVEHALPH-EDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCK 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN718.4e-6844.16E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q93Z928.4e-6845.77E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8LDB81.0e-6544.22E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q8GUU28.7e-6543.49E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q7XTV74.2e-1153.19Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
XP_023539643.11.18e-257100.00E3 ubiquitin-protein ligase At4g11680-like [Cucurbita pepo subsp. pepo][more]
KAG6596184.11.60e-25598.83E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7027721.11.60e-25598.83E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022937857.11.87e-25498.83E3 ubiquitin-protein ligase At4g11680-like [Cucurbita moschata][more]
XP_022971451.12.66e-25499.12E3 ubiquitin-protein ligase At4g11680-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FCD69.06e-25598.83E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1I3D41.29e-25499.12E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1J3G31.66e-20181.82E3 ubiquitin-protein ligase At4g11680-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A5D3DQ813.30e-20180.80E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B8803.30e-20180.80E3 ubiquitin-protein ligase At4g11680-like OS=Cucumis melo OX=3656 GN=LOC1034868... [more]
Match NameE-valueIdentityDescription
AT1G68070.16.4e-7147.72Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.16.0e-6944.16Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.26.0e-6944.16Zinc finger, C3HC4 type (RING finger) family protein [more]
AT4G11680.16.0e-6945.77Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G63170.17.3e-6744.22Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 291..331
e-value: 4.6E-6
score: 36.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 290..332
e-value: 2.8E-12
score: 46.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 291..332
score: 12.238477
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 271..338
e-value: 1.3E-17
score: 64.8
NoneNo IPR availablePANTHERPTHR45977:SF19RING-FINGER UBIQUITIN LIGASEcoord: 18..342
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 18..342
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 290..334
e-value: 2.31643E-18
score: 75.4257
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 282..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g13470.1Cp4.1LG08g13470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane