Cp4.1LG07g06460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG07g06460 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 4.5e-46
Identity = 98/269 (36.43%), Postives = 152/269 (56.51%), Query Frame = 0

Query: 17  PSLSGATSPTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQ 76
           P +     P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIKNP 136
           WE AHEPLH+ ID     G+GPG+ FA+  K +    +  +GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-- 132

Query: 137 SNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRND 196
               +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D
Sbjct: 133 ---GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINLTTFE 256
           +    LP+I V I+    ++          +  E  +  +L +++ +D++ LP+      
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS---- 252

Query: 257 GFSWDHGHFNTETEIVLGKWLADTYLAHY 285
               D+ H  TE ++ LG  LA  YL+++
Sbjct: 253 ----DNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g06460 vs. NCBI nr
Match: XP_023536887.1 (probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536888.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536889.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536890.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023537924.1 probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588 bits (1517), Expect = 3.19e-212
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP
Sbjct: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. NCBI nr
Match: XP_023536885.1 (probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536886.1 probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585 bits (1509), Expect = 5.29e-211
Identity = 287/288 (99.65%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP
Sbjct: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDS ELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 241 TIDSWELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. NCBI nr
Match: XP_022951659.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata] >XP_022951660.1 probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 578 bits (1489), Expect = 5.93e-208
Identity = 281/288 (97.57%), Postives = 285/288 (98.96%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLLKLSILLCMILF+PSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           CQ DPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAG VGLVP
Sbjct: 61  CQFDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDSRELP+N TTFEGFSWDHGHFNTETEIVLGKWLA+TYLAHYGHLL
Sbjct: 241 TIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. NCBI nr
Match: XP_022951911.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 572 bits (1475), Expect = 8.08e-206
Identity = 278/288 (96.53%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLL+LS+LLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLQLSVLLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           C S+PSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAG VGLVP
Sbjct: 61  CLSNPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQW+KNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWMKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDSRELP+N TTFEGFSWD GHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 241 TIDSRELPVNFTTFEGFSWDRGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. NCBI nr
Match: KAG6585849.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564 bits (1454), Expect = 8.54e-203
Identity = 271/277 (97.83%), Postives = 274/277 (98.92%), Query Frame = 0

Query: 12  MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLN 71
           MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLN
Sbjct: 1   MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLN 60

Query: 72  PERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQW 131
           PERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQ KEKAGQKAGTVGLVPCARGGTLIEQW
Sbjct: 61  PERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQLKEKAGQKAGTVGLVPCARGGTLIEQW 120

Query: 132 IKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITD 191
           +KNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITD
Sbjct: 121 MKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITD 180

Query: 192 IRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINL 251
           IRNDIKPRFLPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELP+N 
Sbjct: 181 IRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNF 240

Query: 252 TTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TTFEGFSWDHGHFNTETEIVLGKWLADTYL HYGHLL
Sbjct: 241 TTFEGFSWDHGHFNTETEIVLGKWLADTYLTHYGHLL 277

BLAST of Cp4.1LG07g06460 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 2.87e-208
Identity = 281/288 (97.57%), Postives = 285/288 (98.96%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLLKLSILLCMILF+PSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           CQ DPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAG VGLVP
Sbjct: 61  CQFDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDSRELP+N TTFEGFSWDHGHFNTETEIVLGKWLA+TYLAHYGHLL
Sbjct: 241 TIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. ExPASy TrEMBL
Match: A0A6J1GIT2 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454649 PE=4 SV=1)

HSP 1 Score: 572 bits (1475), Expect = 3.91e-206
Identity = 278/288 (96.53%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLL+LS+LLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE
Sbjct: 1   MVLLQLSVLLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           C S+PSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAG VGLVP
Sbjct: 61  CLSNPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQW+KNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR
Sbjct: 121 CARGGTLIEQWMKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDSRELP+N TTFEGFSWD GHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 241 TIDSRELPVNFTTFEGFSWDRGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g06460 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 9.78e-196
Identity = 268/290 (92.41%), Postives = 275/290 (94.83%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           MVLLKLSIL+C IL SPSLSGATSPTNIFILAGQSNMAGRGGVEK   G+LVWDGKVP E
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDI--SHTPGIGPGIPFAHQFKEKAGQKAGTVGL 120
           CQSDPSILR NPERQWEIAHEPLHLGID+  + TPGIGPGIPFAHQ KEKAGQKAG VGL
Sbjct: 61  CQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGL 120

Query: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180
           VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180

Query: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPD 240
           QRYKDNLKKFITDIRNDIKPRFLPVIIVKI++YDFFMKHDTHNLPAVRAAEDAVQKELPD
Sbjct: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPD 240

Query: 241 IITIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           IITIDS ELP+NLTTFEGFSWDHGHFNT TEI LGKWLADTYLAHYGHLL
Sbjct: 241 IITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Cp4.1LG07g06460 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 533 bits (1374), Expect = 9.73e-191
Identity = 264/288 (91.67%), Postives = 267/288 (92.71%), Query Frame = 0

Query: 1   MVLLKLSILLCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60
           M+LLKLS LLCMILF PSLS ATSPTNIFILAGQSNMAGRGGVE  Q GKL WDGKVPLE
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLE 60

Query: 61  CQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVP 120
           CQSDPSILRLNP RQWEIA EPLHLGIDI  TPGIGPGIPFAHQFK KAGQKAG VGLVP
Sbjct: 61  CQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180
           CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAM+DTA R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHR 180

Query: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240
           YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTH+LPAVRAAEDAVQKELPDII
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288
           TIDS ELPIN TTFEGF  DHGHFNT TEI LGKWLADTYLAHY HLL
Sbjct: 241 TIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Cp4.1LG07g06460 vs. ExPASy TrEMBL
Match: A0A6J1GK48 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454647 PE=4 SV=1)

HSP 1 Score: 501 bits (1290), Expect = 1.65e-178
Identity = 239/252 (94.84%), Postives = 242/252 (96.03%), Query Frame = 0

Query: 37  MAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDISHTPGIG 96
           MAGRGGVEK QNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDI HTPGIG
Sbjct: 1   MAGRGGVEKTQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDIGHTPGIG 60

Query: 97  PGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 156
            GIPFAHQ KEKAGQKAG VGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE
Sbjct: 61  SGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120

Query: 157 GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMK 216
           GGVVRALFWYQGESDAAMNDTAQRYK+NLKKFITDIRNDIKPRFLPVIIVKI++YDFFMK
Sbjct: 121 GGVVRALFWYQGESDAAMNDTAQRYKENLKKFITDIRNDIKPRFLPVIIVKIALYDFFMK 180

Query: 217 HDTHNLPAVRAAEDAVQKELPDIITIDSRELPINLTTFEGFSWDHGHFNTETEIVLGKWL 276
           HDTHNLPAVR AEDAVQKELPDIITIDS ELPINLTTFEGFSWDHGH NT TEI LGKWL
Sbjct: 181 HDTHNLPAVREAEDAVQKELPDIITIDSLELPINLTTFEGFSWDHGHLNTATEIALGKWL 240

Query: 277 ADTYLAHYGHLL 288
           ADTYLAHYGHLL
Sbjct: 241 ADTYLAHYGHLL 252

BLAST of Cp4.1LG07g06460 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 98/269 (36.43%), Postives = 152/269 (56.51%), Query Frame = 0

Query: 17  PSLSGATSPTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQ 76
           P +     P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIKNP 136
           WE AHEPLH+ ID     G+GPG+ FA+  K +    +  +GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-- 132

Query: 137 SNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRND 196
               +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D
Sbjct: 133 ---GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINLTTFE 256
           +    LP+I V I+    ++          +  E  +  +L +++ +D++ LP+      
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS---- 252

Query: 257 GFSWDHGHFNTETEIVLGKWLADTYLAHY 285
               D+ H  TE ++ LG  LA  YL+++
Sbjct: 253 ----DNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g06460 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 98/269 (36.43%), Postives = 152/269 (56.51%), Query Frame = 0

Query: 17  PSLSGATSPTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQ 76
           P +     P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIKNP 136
           WE AHEPLH+ ID     G+GPG+ FA+  K +    +  +GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-- 132

Query: 137 SNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRND 196
               +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D
Sbjct: 133 ---GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINLTTFE 256
           +    LP+I V I+    ++          +  E  +  +L +++ +D++ LP+      
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS---- 252

Query: 257 GFSWDHGHFNTETEIVLGKWLADTYLAHY 285
               D+ H  TE ++ LG  LA  YL+++
Sbjct: 253 ----DNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g06460 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 185.3 bits (469), Expect = 7.1e-47
Identity = 111/271 (40.96%), Postives = 154/271 (56.83%), Query Frame = 0

Query: 17  PSLSGATSPTN--IFILAGQSNMAGRGGV-EKIQNGKLVWDGKVPLECQSDPSILRLNPE 76
           P L   T   N  IFILAGQSNMAGRGGV         VWDG +P EC+S+PSILRL  +
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 77  RQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIK 136
            +W+ A EPLH+ IDI+ T G+GPG+PFA++   + GQ    VGLVPC+ GGT + QW K
Sbjct: 78  LEWKEAKEPLHVDIDINKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK 137

Query: 137 NPSNPSATFYQNFIERIKTSEKE--GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITD 196
                    Y+  ++R K +     GG  RA+ WYQGESD      A  YK  L KF +D
Sbjct: 138 -----GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSD 197

Query: 197 IRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINL 256
           +RND++   LP+I V ++            L AVR A+  ++ +L ++  +D+R LP+  
Sbjct: 198 LRNDLQHPNLPIIQVALA------TGAGPYLDAVRKAQ--LKTDLENVYCVDARGLPLEP 257

Query: 257 TTFEGFSWDHGHFNTETEIVLGKWLADTYLA 283
                   D  H  T +++ LG  +A+++LA
Sbjct: 258 --------DGLHLTTSSQVQLGHMIAESFLA 263