Cp4.1LG07g03250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG07g03250 vs. ExPASy Swiss-Prot
Match: Q5FV41 (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.0e-145
Identity = 274/498 (55.02%), Postives = 345/498 (69.28%), Query Frame = 0

Query: 1   MMVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL 60
           M+VEE+ L+ +   +   + +      +   + W K+L +E+HWSFV GVV +YGI+QGL
Sbjct: 1   MVVEEQSLE-NGGSVHVVKGESNFRNVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGL 60

Query: 61  GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV 120
           GG+L RVAT+YYMKDVQKVQPS +Q  + IT IPW++KP+WG+ TD++PIFG+HRRPYF+
Sbjct: 61  GGSLGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFI 120

Query: 121 FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL 180
            AG+LGV+SLL +SL   LHL LA+  +T+ SA +AIADVTIDAC A NS  HP+LA+D+
Sbjct: 121 LAGVLGVVSLLFISLHSNLHLYLALFWMTISSAAMAIADVTIDACTAYNSIKHPSLASDM 180

Query: 181 QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR 240
           QSLC+LSSSIGALLGF +SGI VHL+G KGV+GLL  P  LV +VG++ +EPH+P F+Y+
Sbjct: 181 QSLCSLSSSIGALLGFFMSGILVHLVGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYK 240

Query: 241 ---------------------------------------------------------QEN 300
                                                                    QE 
Sbjct: 241 QVNQKFTDAGKAMWRTMKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQET 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP 360
           VGFI SIGS+GS+L A LYQ VLKDH FR L  WTQL+F+LSGMLD +LV RLNLKFGLP
Sbjct: 301 VGFILSIGSIGSILAATLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLP 360

Query: 361 DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYLFIV+DE V Q++ RLKWMPLLVL+SKLCP GIEGTFFALLMSIDN GL+++SW GG 
Sbjct: 361 DYLFIVVDEIVSQMIGRLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGI 420

Query: 421 LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS 439
           +LH+L VTRT+F NLWLA+L+RN +RL PLC LFLVP+GD N+  L  E+   D D E  
Sbjct: 421 MLHVLKVTRTEFGNLWLAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDEIMGEDSDEEKD 480

BLAST of Cp4.1LG07g03250 vs. ExPASy Swiss-Prot
Match: F4KIL8 (Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana OX=3702 GN=At5g25040 PE=2 SV=2)

HSP 1 Score: 453.4 bits (1165), Expect = 3.0e-126
Identity = 244/460 (53.04%), Postives = 311/460 (67.61%), Query Frame = 0

Query: 34  WFKVLVAEMHWSFVLGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSI 93
           W K+L +++HWS+V  VV   GI+QG G +L  VAT YYMKDVQKVQPS  Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLFTDLVPIFGYHRRPYFVFAGLLGVISLLSLSLLEKLHLPLAILLLTVGS 153
            W++ KP++G+ TD++PIFG+HRRPYF+ AG++GV+SLL +SL   LHL LA+  +T+ S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAIADVTIDACVAQNSSFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVY 213
           A +AIADVTIDAC   NS+ HP+LA+D+QSLC+LS SIG LLGF +SGI VHL+G KGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPGGLVLLVGVLLNEPHMPNFNYR-------------------------------- 273
           GLL     LV +VGVL +EP +  F+++                                
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFITRAL 272

Query: 274 ----------------------QENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWT 333
                                 QE VGFI SIGS+GS+LG LLY   LKDH FR L  WT
Sbjct: 273 GLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLFLWT 332

Query: 334 QLIFSLSGMLDFLLVLRLNLKFGLPDYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGI 393
           QL+F+LSGM D +LVLRLNLKFGLPDYLFIV+D  V +++ RL WM + VL++KLCP GI
Sbjct: 333 QLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCPHGI 392

Query: 394 EGTFFALLMSIDNVGLLSASWGGGFLLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFL 439
           EGTFFALLMSIDN GL+++SW GG +LH+L VTRT+F NLWLA+L+RN +RL P+C LFL
Sbjct: 393 EGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRTEFGNLWLAVLVRNVMRLLPICFLFL 452

BLAST of Cp4.1LG07g03250 vs. ExPASy Swiss-Prot
Match: Q9SQN2 (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 5.4e-123
Identity = 238/489 (48.67%), Postives = 321/489 (65.64%), Query Frame = 0

Query: 4   EEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLGGA 63
           E E    S  + ++ + +G     +   L W ++L+ E+HWSF  GV+IVYG+SQGLG  
Sbjct: 7   EAEYENDSRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKG 66

Query: 64  LNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVFAG 123
           L++V+T+YY KD QK+QPS AQ Y G+  IPW++KP+WGL TD+VP+ GY RRPYF+ AG
Sbjct: 67  LSKVSTQYYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAG 126

Query: 124 LLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQSL 183
            L +IS++ L L   LHL LA+  L  GSAGVAIADVTIDACV Q S  HPTLAAD+QSL
Sbjct: 127 FLAMISMMVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSL 186

Query: 184 CALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEP----------- 243
           C L SSIG+L+GFS+SG+ VHL+G KGVYGLL +  GL+++VG++L E            
Sbjct: 187 CGLCSSIGSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVN 246

Query: 244 ------------------------------------HMPNF----------NYRQENVGF 303
                                               H   F          ++ +E VG 
Sbjct: 247 DKFLDAGSAIWKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGS 306

Query: 304 IFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPDYL 363
           I S G++GSL+G LLYQ  LK+  FR+++FW   +  LSG LD +LVLR+NLK GLPDY 
Sbjct: 307 IMSFGAIGSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYF 366

Query: 364 FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFLLH 423
           FIV+DE V  +++R+KW+PLLVLSSKLCP G+EGTFFALLMSI+++G L +SWGGG LLH
Sbjct: 367 FIVVDEFVSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLH 426

Query: 424 ILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISEAN 436
            L VTRT+F NLWL I++R+ LR+ P+ ++FL+P  DSNS+IL  E+ +S +   + +  
Sbjct: 427 ALKVTRTQFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGV-DTE 486

BLAST of Cp4.1LG07g03250 vs. ExPASy Swiss-Prot
Match: Q9LEV7 (Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana OX=3702 GN=At5g10820 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.1e-95
Identity = 193/464 (41.59%), Postives = 285/464 (61.42%), Query Frame = 0

Query: 34  WFKVLVAEMHWSFVLGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSI 93
           W ++L + ++ SFVLGVV+VYG++QG  G++ +V T YY KDVQ+VQPS  Q Y G+  I
Sbjct: 45  WLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFKVVTDYYWKDVQQVQPSVVQLYMGLYYI 104

Query: 94  PWMVKPIWGLFTDLVPIFGYHRRPYFVFAGLLGVISLLSLSLLEKLHLPLAILLLTVGSA 153
           PW+++PIWGLFTD+ PI GY R+PYFV +G+LG++S +++ +L KL   LA+  L   SA
Sbjct: 105 PWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLGLVSAIAIVVLGKLPAALALSCLLGVSA 164

Query: 154 GVAIADVTIDACVAQNSSFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVYG 213
            +AIADV IDAC+A NS    +LA D+QSLC + SS GAL+G++ SG+FVH +GP+G  G
Sbjct: 165 AMAIADVVIDACIATNSINIRSLAPDIQSLCMVCSSAGALVGYATSGVFVHQLGPQGALG 224

Query: 214 LLAIPGGLVLLVGVLLNE------------------------------------------ 273
           +LA     ++++G  + E                                          
Sbjct: 225 VLAFSPATIVILGFFIYEKRSSTVPTQKTKKDTDGLGVAVKGMCKTVKYPEVWKPSLYMF 284

Query: 274 --------PHMPNF----------NYRQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRD 333
                    H  +F           + QE VG I+++G++ S+ G L+Y   LK + FR+
Sbjct: 285 ISLALNISTHEGHFYWYTDPTAGPAFSQEFVGIIYAVGALASMFGVLIYHKKLKGYSFRN 344

Query: 334 LLFWTQLIFSLSGMLDFLLVLRLNLKFGLPDYLFIVIDESVHQLVNRLKWMPLLVLSSKL 393
           +LF+ QL++  SGMLD + + R NL  G+PD LF++ +ES  +++++++W+P++VLS++L
Sbjct: 345 ILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSLFVITEESFTKMISKIRWIPMVVLSTRL 404

Query: 394 CPRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILNVTRTKFHNLWLAILLRNFLRLAPL 434
           CP GIEGTFFA LM ID+ G L++ WGGGF+LH   VTR  F NLWL IL+RN LRL  +
Sbjct: 405 CPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLHAFGVTRHDFGNLWLVILIRNILRLVTV 464

BLAST of Cp4.1LG07g03250 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-37
Identity = 132/441 (29.93%), Postives = 201/441 (45.58%), Query Frame = 0

Query: 48  LGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDL 107
           + V +VY +   LG  L R+A  +Y+KD   + P+     +G++S+PW+VKP++G  +D 
Sbjct: 128 VAVAMVYFVQGVLG--LARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDS 187

Query: 108 VPIFGYHRRPYFVFAGLLGVI--SLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDAC 167
           VP+FGY RR Y V +GLLG    SL++  +  K      ILL   GS  VA +DV +D+ 
Sbjct: 188 VPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILL---GSLSVAFSDVVVDSM 247

Query: 168 VAQNS-SFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLL 227
           V + +     +++  LQSLC  SS+ G ++    SG  V   G + V+G+ A+   +   
Sbjct: 248 VVERARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSA 307

Query: 228 VGVLLNE----------------------------------------------------- 287
           V VL+NE                                                     
Sbjct: 308 VAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIFLWQA 367

Query: 288 -PHMPN---------FNYRQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLI 347
            PH  +           +  E +G +  + S+ SLLG  LY   LK    R +   T + 
Sbjct: 368 TPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLVTTIF 427

Query: 348 FSLSGMLDFLLVLRLNLKFGLPDYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGT 407
            +  GM   +LV   N + G+ D  F + D  +  ++ +  +MP+LVL+++LCP G+E T
Sbjct: 428 GTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEGMEAT 487

Query: 408 FFALLMSIDNVGLLSASWGGGFLLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPR 423
            FA LMSI N G +     G  L     +TR  F NL   I+L N   L PL +L L+PR
Sbjct: 488 LFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLGLLPR 547

BLAST of Cp4.1LG07g03250 vs. NCBI nr
Match: XP_023538502.1 (probable folate-biopterin transporter 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833 bits (2153), Expect = 6.98e-303
Identity = 446/503 (88.67%), Postives = 446/503 (88.67%), Query Frame = 0

Query: 1   MMVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL 60
           MMVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL
Sbjct: 1   MMVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL 60

Query: 61  GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV 120
           GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV
Sbjct: 61  GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV 120

Query: 121 FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL 180
           FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL
Sbjct: 121 FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL 180

Query: 181 QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR 240
           QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR 240

Query: 241 Q---------------------------------------------------------EN 300
           Q                                                         EN
Sbjct: 241 QVSDKFVSAGKAMWMTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP 360
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP 360

Query: 361 DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS 446
           LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS
Sbjct: 421 LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS 480

BLAST of Cp4.1LG07g03250 vs. NCBI nr
Match: KAG6585504.1 (putative folate-biopterin transporter 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020420.1 putative folate-biopterin transporter 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 826 bits (2133), Expect = 7.48e-300
Identity = 440/502 (87.65%), Postives = 443/502 (88.25%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG
Sbjct: 1   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF
Sbjct: 61  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSLSLLE LHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ
Sbjct: 121 AGLLGVISLLSLSLLENLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVG+LLNEPHMPNFNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGLLLNEPHMPNFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VSDKFVSAGKAMWTTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHILNVTRTKFHNLWLAIL+RNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNE+SE
Sbjct: 421 LHILNVTRTKFHNLWLAILIRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEVSE 480

BLAST of Cp4.1LG07g03250 vs. NCBI nr
Match: XP_022951179.1 (probable folate-biopterin transporter 2 [Cucurbita moschata] >XP_022951180.1 probable folate-biopterin transporter 2 [Cucurbita moschata] >XP_022951182.1 probable folate-biopterin transporter 2 [Cucurbita moschata])

HSP 1 Score: 821 bits (2121), Expect = 5.04e-298
Identity = 438/502 (87.25%), Postives = 442/502 (88.05%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG
Sbjct: 1   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF
Sbjct: 61  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSLSLLE LHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ
Sbjct: 121 AGLLGVISLLSLSLLENLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVG+LLNEPHMPNFNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGLLLNEPHMPNFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VSDKFVSAGKAMWTTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHILNVTRTKF NLWLAIL+RNFLRLAPLCMLFLVPRGDSNSSILITELPSSD+D E+SE
Sbjct: 421 LHILNVTRTKFDNLWLAILIRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDIDTEVSE 480

BLAST of Cp4.1LG07g03250 vs. NCBI nr
Match: XP_023002314.1 (probable folate-biopterin transporter 2 [Cucurbita maxima] >XP_023002315.1 probable folate-biopterin transporter 2 [Cucurbita maxima])

HSP 1 Score: 818 bits (2114), Expect = 5.86e-297
Identity = 438/502 (87.25%), Postives = 440/502 (87.65%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLKV AEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG
Sbjct: 1   MVEEEKLKVPAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF
Sbjct: 61  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ
Sbjct: 121 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VSEKFVSAGRAMWMTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLK HQF+DLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKGHQFQDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           Y FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHILNVTRTKFHNLWLAIL+RN LRL PLCMLFLVPRGDSNSSILITELPSSDVDNEISE
Sbjct: 421 LHILNVTRTKFHNLWLAILIRNCLRLVPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 480

BLAST of Cp4.1LG07g03250 vs. NCBI nr
Match: XP_008445272.1 (PREDICTED: probable folate-biopterin transporter 2 [Cucumis melo] >KAA0064880.1 putative folate-biopterin transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 748 bits (1931), Expect = 4.47e-269
Identity = 405/503 (80.52%), Postives = 419/503 (83.30%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLK+S EDLEEDEPQ ES   IG ALYWFK+LV EMHWSFV GVVIVYGISQGLG
Sbjct: 1   MVEEEKLKISVEDLEEDEPQKESNVSIGSALYWFKLLVTEMHWSFVFGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRV+TKYYMKDVQKVQPS AQ YSGITSIPWMVKPIWGLFTDLVPI GYHRRPYFVF
Sbjct: 61  GALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPILGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSL+L EKLHL LAILLLT GSA VAIADVTIDACVAQNS+ HPTLAADLQ
Sbjct: 121 AGLLGVISLLSLALHEKLHLVLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGI VHLIG KGVYGLLAIPGGLV LVG+LLNEPHMP+FNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VNEKFVGAGKAMWSTLKIPDVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+PD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           Y FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHIL VTRTKF NLWLAIL+RN LRL+PLCMLFLVPRGD NSSIL TELPSS+VDN+ SE
Sbjct: 421 LHILQVTRTKFSNLWLAILIRNLLRLSPLCMLFLVPRGDPNSSILPTELPSSEVDNDTSE 480

BLAST of Cp4.1LG07g03250 vs. ExPASy TrEMBL
Match: A0A6J1GHZ0 (probable folate-biopterin transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111454103 PE=3 SV=1)

HSP 1 Score: 821 bits (2121), Expect = 2.44e-298
Identity = 438/502 (87.25%), Postives = 442/502 (88.05%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG
Sbjct: 1   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF
Sbjct: 61  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSLSLLE LHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ
Sbjct: 121 AGLLGVISLLSLSLLENLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVG+LLNEPHMPNFNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGLLLNEPHMPNFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VSDKFVSAGKAMWTTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHILNVTRTKF NLWLAIL+RNFLRLAPLCMLFLVPRGDSNSSILITELPSSD+D E+SE
Sbjct: 421 LHILNVTRTKFDNLWLAILIRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDIDTEVSE 480

BLAST of Cp4.1LG07g03250 vs. ExPASy TrEMBL
Match: A0A6J1KQ38 (probable folate-biopterin transporter 2 OS=Cucurbita maxima OX=3661 GN=LOC111496189 PE=3 SV=1)

HSP 1 Score: 818 bits (2114), Expect = 2.84e-297
Identity = 438/502 (87.25%), Postives = 440/502 (87.65%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLKV AEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG
Sbjct: 1   MVEEEKLKVPAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF
Sbjct: 61  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ
Sbjct: 121 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VSEKFVSAGRAMWMTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLK HQF+DLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKGHQFQDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           Y FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHILNVTRTKFHNLWLAIL+RN LRL PLCMLFLVPRGDSNSSILITELPSSDVDNEISE
Sbjct: 421 LHILNVTRTKFHNLWLAILIRNCLRLVPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 480

BLAST of Cp4.1LG07g03250 vs. ExPASy TrEMBL
Match: A0A5A7VCG2 (Putative folate-biopterin transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001930 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 2.16e-269
Identity = 405/503 (80.52%), Postives = 419/503 (83.30%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLK+S EDLEEDEPQ ES   IG ALYWFK+LV EMHWSFV GVVIVYGISQGLG
Sbjct: 1   MVEEEKLKISVEDLEEDEPQKESNVSIGSALYWFKLLVTEMHWSFVFGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRV+TKYYMKDVQKVQPS AQ YSGITSIPWMVKPIWGLFTDLVPI GYHRRPYFVF
Sbjct: 61  GALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPILGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSL+L EKLHL LAILLLT GSA VAIADVTIDACVAQNS+ HPTLAADLQ
Sbjct: 121 AGLLGVISLLSLALHEKLHLVLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGI VHLIG KGVYGLLAIPGGLV LVG+LLNEPHMP+FNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VNEKFVGAGKAMWSTLKIPDVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+PD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           Y FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHIL VTRTKF NLWLAIL+RN LRL+PLCMLFLVPRGD NSSIL TELPSS+VDN+ SE
Sbjct: 421 LHILQVTRTKFSNLWLAILIRNLLRLSPLCMLFLVPRGDPNSSILPTELPSSEVDNDTSE 480

BLAST of Cp4.1LG07g03250 vs. ExPASy TrEMBL
Match: A0A1S3BC95 (probable folate-biopterin transporter 2 OS=Cucumis melo OX=3656 GN=LOC103488355 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 2.16e-269
Identity = 405/503 (80.52%), Postives = 419/503 (83.30%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLG 61
           MVEEEKLK+S EDLEEDEPQ ES   IG ALYWFK+LV EMHWSFV GVVIVYGISQGLG
Sbjct: 1   MVEEEKLKISVEDLEEDEPQKESNVSIGSALYWFKLLVTEMHWSFVFGVVIVYGISQGLG 60

Query: 62  GALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVF 121
           GALNRV+TKYYMKDVQKVQPS AQ YSGITSIPWMVKPIWGLFTDLVPI GYHRRPYFVF
Sbjct: 61  GALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPILGYHRRPYFVF 120

Query: 122 AGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQ 181
           AGLLGVISLLSL+L EKLHL LAILLLT GSA VAIADVTIDACVAQNS+ HPTLAADLQ
Sbjct: 121 AGLLGVISLLSLALHEKLHLVLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADLQ 180

Query: 182 SLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYRQ 241
           SLCALSSSIGALLGFSISGI VHLIG KGVYGLLAIPGGLV LVG+LLNEPHMP+FNYRQ
Sbjct: 181 SLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYRQ 240

Query: 242 ---------------------------------------------------------ENV 301
                                                                    ENV
Sbjct: 241 VNEKFVGAGKAMWSTLKIPDVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQENV 300

Query: 302 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPD 361
           GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+PD
Sbjct: 301 GFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIPD 360

Query: 362 YLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 421
           Y FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL
Sbjct: 361 YFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFL 420

Query: 422 LHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISE 446
           LHIL VTRTKF NLWLAIL+RN LRL+PLCMLFLVPRGD NSSIL TELPSS+VDN+ SE
Sbjct: 421 LHILQVTRTKFSNLWLAILIRNLLRLSPLCMLFLVPRGDPNSSILPTELPSSEVDNDTSE 480

BLAST of Cp4.1LG07g03250 vs. ExPASy TrEMBL
Match: A0A6J1BS52 (probable folate-biopterin transporter 2 OS=Momordica charantia OX=3673 GN=LOC111005061 PE=3 SV=1)

HSP 1 Score: 738 bits (1905), Expect = 1.96e-265
Identity = 397/501 (79.24%), Postives = 416/501 (83.03%), Query Frame = 0

Query: 2   MVEEEKLKVSAEDLEE-DEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL 61
           MVEEEKLK SAE LEE DEPQ ESGGCIG ALYWF++LV EMHWSFVLG+VIVYGISQGL
Sbjct: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60

Query: 62  GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV 121
           GGALNRVAT+YYMKDVQKVQPS AQ YSGITSIPWMVKPIWGL TDLVPI GY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120

Query: 122 FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL 181
            AGLLGVISLLSL+L EKLHL LAILLLT GSAGVA+ADVTIDACVAQNSS HPTLAADL
Sbjct: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180

Query: 182 QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR 241
           QSLCALSSS+GALLGFSISGI VHLIG KGVYGLLAIP GLVLLVG+LLNEPH+P+FNYR
Sbjct: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240

Query: 242 Q---------------------------------------------------------EN 301
           Q                                                         EN
Sbjct: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPNFSQEN 300

Query: 302 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP 361
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+P
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360

Query: 362 DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 421
           DY FIVIDESVHQL+NRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 422 LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS 444
           LLHIL VTRTKF NLWLAIL+RN LRL PLCMLFLVPRGD NSSIL+TELPS +VD+E  
Sbjct: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480

BLAST of Cp4.1LG07g03250 vs. TAIR 10
Match: AT5G25050.1 (Major facilitator superfamily protein )

HSP 1 Score: 515.8 bits (1327), Expect = 3.5e-146
Identity = 274/498 (55.02%), Postives = 345/498 (69.28%), Query Frame = 0

Query: 1   MMVEEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGL 60
           M+VEE+ L+ +   +   + +      +   + W K+L +E+HWSFV GVV +YGI+QGL
Sbjct: 1   MVVEEQSLE-NGGSVHVVKGESNFRNVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGL 60

Query: 61  GGALNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFV 120
           GG+L RVAT+YYMKDVQKVQPS +Q  + IT IPW++KP+WG+ TD++PIFG+HRRPYF+
Sbjct: 61  GGSLGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFI 120

Query: 121 FAGLLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADL 180
            AG+LGV+SLL +SL   LHL LA+  +T+ SA +AIADVTIDAC A NS  HP+LA+D+
Sbjct: 121 LAGVLGVVSLLFISLHSNLHLYLALFWMTISSAAMAIADVTIDACTAYNSIKHPSLASDM 180

Query: 181 QSLCALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEPHMPNFNYR 240
           QSLC+LSSSIGALLGF +SGI VHL+G KGV+GLL  P  LV +VG++ +EPH+P F+Y+
Sbjct: 181 QSLCSLSSSIGALLGFFMSGILVHLVGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYK 240

Query: 241 ---------------------------------------------------------QEN 300
                                                                    QE 
Sbjct: 241 QVNQKFTDAGKAMWRTMKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQET 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLP 360
           VGFI SIGS+GS+L A LYQ VLKDH FR L  WTQL+F+LSGMLD +LV RLNLKFGLP
Sbjct: 301 VGFILSIGSIGSILAATLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLP 360

Query: 361 DYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYLFIV+DE V Q++ RLKWMPLLVL+SKLCP GIEGTFFALLMSIDN GL+++SW GG 
Sbjct: 361 DYLFIVVDEIVSQMIGRLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGI 420

Query: 421 LLHILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEIS 439
           +LH+L VTRT+F NLWLA+L+RN +RL PLC LFLVP+GD N+  L  E+   D D E  
Sbjct: 421 MLHVLKVTRTEFGNLWLAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDEIMGEDSDEEKD 480

BLAST of Cp4.1LG07g03250 vs. TAIR 10
Match: AT5G25040.2 (Major facilitator superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-124
Identity = 244/485 (50.31%), Postives = 311/485 (64.12%), Query Frame = 0

Query: 34  WFKVLVAEMHWSFVLGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSI 93
           W K+L +++HWS+V  VV   GI+QG G +L  VAT YYMKDVQKVQPS  Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLFTDLVPIFGYHRRPYFVFAGLLGVISLLSLSLLEKLHLPLAILLLTVGS 153
            W++ KP++G+ TD++PIFG+HRRPYF+ AG++GV+SLL +SL   LHL LA+  +T+ S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAIADVTIDACVAQNSSFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVY 213
           A +AIADVTIDAC   NS+ HP+LA+D+QSLC+LS SIG LLGF +SGI VHL+G KGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPGGLVLLVGVLLNEPHMPNFNYR-------------------------------- 273
           GLL     LV +VGVL +EP +  F+++                                
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFITRAL 272

Query: 274 -----------------------------------------------QENVGFIFSIGSV 333
                                                          QE VGFI SIGS+
Sbjct: 273 GLDIKEGLFYWFTDSKDGPLFAQVIPKQVASIFSKSTNMLKAFLVCFQETVGFILSIGSI 332

Query: 334 GSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPDYLFIVIDES 393
           GS+LG LLY   LKDH FR L  WTQL+F+LSGM D +LVLRLNLKFGLPDYLFIV+D  
Sbjct: 333 GSILGVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGI 392

Query: 394 VHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILNVTRT 439
           V +++ RL WM + VL++KLCP GIEGTFFALLMSIDN GL+++SW GG +LH+L VTRT
Sbjct: 393 VSKMIIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRT 452

BLAST of Cp4.1LG07g03250 vs. TAIR 10
Match: AT1G79710.1 (Major facilitator superfamily protein )

HSP 1 Score: 442.6 bits (1137), Expect = 3.8e-124
Identity = 238/489 (48.67%), Postives = 321/489 (65.64%), Query Frame = 0

Query: 4   EEEKLKVSAEDLEEDEPQGESGGCIGMALYWFKVLVAEMHWSFVLGVVIVYGISQGLGGA 63
           E E    S  + ++ + +G     +   L W ++L+ E+HWSF  GV+IVYG+SQGLG  
Sbjct: 7   EAEYENDSRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKG 66

Query: 64  LNRVATKYYMKDVQKVQPSGAQFYSGITSIPWMVKPIWGLFTDLVPIFGYHRRPYFVFAG 123
           L++V+T+YY KD QK+QPS AQ Y G+  IPW++KP+WGL TD+VP+ GY RRPYF+ AG
Sbjct: 67  LSKVSTQYYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAG 126

Query: 124 LLGVISLLSLSLLEKLHLPLAILLLTVGSAGVAIADVTIDACVAQNSSFHPTLAADLQSL 183
            L +IS++ L L   LHL LA+  L  GSAGVAIADVTIDACV Q S  HPTLAAD+QSL
Sbjct: 127 FLAMISMMVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSL 186

Query: 184 CALSSSIGALLGFSISGIFVHLIGPKGVYGLLAIPGGLVLLVGVLLNEP----------- 243
           C L SSIG+L+GFS+SG+ VHL+G KGVYGLL +  GL+++VG++L E            
Sbjct: 187 CGLCSSIGSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVN 246

Query: 244 ------------------------------------HMPNF----------NYRQENVGF 303
                                               H   F          ++ +E VG 
Sbjct: 247 DKFLDAGSAIWKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGS 306

Query: 304 IFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGLPDYL 363
           I S G++GSL+G LLYQ  LK+  FR+++FW   +  LSG LD +LVLR+NLK GLPDY 
Sbjct: 307 IMSFGAIGSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYF 366

Query: 364 FIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFLLH 423
           FIV+DE V  +++R+KW+PLLVLSSKLCP G+EGTFFALLMSI+++G L +SWGGG LLH
Sbjct: 367 FIVVDEFVSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLH 426

Query: 424 ILNVTRTKFHNLWLAILLRNFLRLAPLCMLFLVPRGDSNSSILITELPSSDVDNEISEAN 436
            L VTRT+F NLWL I++R+ LR+ P+ ++FL+P  DSNS+IL  E+ +S +   + +  
Sbjct: 427 ALKVTRTQFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGV-DTE 486

BLAST of Cp4.1LG07g03250 vs. TAIR 10
Match: AT5G25040.3 (Major facilitator superfamily protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.6e-109
Identity = 210/396 (53.03%), Postives = 266/396 (67.17%), Query Frame = 0

Query: 34  WFKVLVAEMHWSFVLGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSI 93
           W K+L +++HWS+V  VV   GI+QG G +L  VAT YYMKDVQKVQPS  Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLFTDLVPIFGYHRRPYFVFAGLLGVISLLSLSLLEKLHLPLAILLLTVGS 153
            W++ KP++G+ TD++PIFG+HRRPYF+ AG++GV+SLL +SL   LHL LA+  +T+ S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAIADVTIDACVAQNSSFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVY 213
           A +AIADVTIDAC   NS+ HP+LA+D+QSLC+LS SIG LLGF +SGI VHL+G KGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPGGLVLLVGVLLNEPHMPNFNYR-------------------------------- 273
           GLL     LV +VGVL +EP +  F+++                                
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFITRAL 272

Query: 274 ----------------------QENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWT 333
                                 QE VGFI SIGS+GS+LG LLY   LKDH FR L  WT
Sbjct: 273 GLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLFLWT 332

Query: 334 QLIFSLSGMLDFLLVLRLNLKFGLPDYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGI 375
           QL+F+LSGM D +LVLRLNLKFGLPDYLFIV+D  V +++ RL WM + VL++KLCP GI
Sbjct: 333 QLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCPHGI 392

BLAST of Cp4.1LG07g03250 vs. TAIR 10
Match: AT5G25040.1 (Major facilitator superfamily protein )

HSP 1 Score: 390.6 bits (1002), Expect = 1.7e-108
Identity = 209/393 (53.18%), Postives = 264/393 (67.18%), Query Frame = 0

Query: 34  WFKVLVAEMHWSFVLGVVIVYGISQGLGGALNRVATKYYMKDVQKVQPSGAQFYSGITSI 93
           W K+L +++HWS+V  VV   GI+QG G +L  VAT YYMKDVQKVQPS  Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLFTDLVPIFGYHRRPYFVFAGLLGVISLLSLSLLEKLHLPLAILLLTVGS 153
            W++ KP++G+ TD++PIFG+HRRPYF+ AG++GV+SLL +SL   LHL LA+  +T+ S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAIADVTIDACVAQNSSFHPTLAADLQSLCALSSSIGALLGFSISGIFVHLIGPKGVY 213
           A +AIADVTIDAC   NS+ HP+LA+D+QSLC+LS SIG LLGF +SGI VHL+G KGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPGGLVLLVGVLLNEPHMPNFNYR-------------------------------- 273
           GLL     LV +VGVL +EP +  F+++                                
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFITRAL 272

Query: 274 ----------------------QENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWT 333
                                 QE VGFI SIGS+GS+LG LLY   LKDH FR L  WT
Sbjct: 273 GLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLFLWT 332

Query: 334 QLIFSLSGMLDFLLVLRLNLKFGLPDYLFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGI 372
           QL+F+LSGM D +LVLRLNLKFGLPDYLFIV+D  V +++ RL WM + VL++KLCP GI
Sbjct: 333 QLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCPHGI 392