Cp4.1LG07g02730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g02730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin-protein ligase
LocationCp4.1LG07: 1750519 .. 1752316 (-)
RNA-Seq ExpressionCp4.1LG07g02730
SyntenyCp4.1LG07g02730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTCCCTCGGTGGAACTAAATTGTGAAAGCGAAGAAGACTCTTACCCTTTATTAATGGCCCAGCCAGAAAACATCAGCAGCAGTGAGCATATCATTAACATAACTGGTACTGGTGATTCTGCTCCCTCTAGTTTGCCCCATGGTAGGAATTCAAATGGCTTAAATTCATCACAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCTTCGTCTCAACCTTCAATTTCTACTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATTCGCGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTGCTACTCACTATGAGCCAGATTATTGCAGCTATTATAGTATTATCATTGTCAAAGAATGAAAAACCTCGTGCACCTTTGTTTGCATGGATTGTGGGATATGCATCTGGGTGTGGTGCGACACTTCCTCTTCTCTATTGGCGTTATCGACACCGTAATCAGGCTTCAGAACAAGATTCTCTTCAACCTAGTCAGAATTCTAATCGCATTAATGTTCCTGCTGGGCCATTCTCTGTTTCAGTTTCTAGGGCATCAGAAGGCGAAGAACTTCATCATCCTGCCCCATCCCCCAGAGGCAGTGTAGGGTCAGGATTACTGACAGCAAGGTATTGGTTTTTTAATGCTTGTACCAGTATAAAATTTAATAATTGGACTTGATTGGTAGATAAATTAATTTCTGCTATCTTGTGGCATTACTTTTAGATGTATCGGTATTTGGTTTTTCAGTTCACAAGATTAGTTCATTGATTTATACTTGCTGATAGCTATTTCTACGCTTATTGGTTTTGAAATCATGAAAGCTGAGTTGGGAGGGAGAATCTATTCGCACACCTTTTATCTTCTTTTTCATTCCCATACTTAAATTTTCGAACTATGCAAGAGATCCTAATTCCCTACATTTTTACATCTTATTGAAGCTGTTCAACTTACCTCATAAGGGCATGTCAGTCTCAGTTGGCAGGTTGCCATTTTCACTGGATCACAGATTCATCATCACGCGTTTATTTTATTCCTTTCTCTTGGACTTAGTGACGATATGTATGCCATATTGCCATGACCTTGACCTAGATTGATTAAATATCAAATGTCTATGATAATGACGGCCAAGGGTGTGGCAGTCTCTTGCTTGCCATAGCTACTCAATTCCCTGTTTCTTTATCTTTTCATGAGGCATTTTTTCCCTTCTTTTCTCCAGAAGTTGAGCAATAATTGCCCTTACAGTTGGGACTTGTGGTAATTTTGACTGCAGGGTAAAAGTTTTGGTTGAATATTTTAAGATGGGCCTGGATTGCTTCTTTGCTATTTGGTTTGTAGTTGGTAACGTTTGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAATTTGTACAGGTCCGTGTTCTTACACCATATGCATATGTAGACGATGCATACGATTGTTTCTAGAATGGATTTATTAATTTCTTCCTCTTTACTTGCAGGCTATGTATAGTGTTTCTTACCTTTAGCTGTATTGGTTATGCCATGCCCTTCATTCTATGTGTAACCATATGCTGTTGCCTACCTTGTATAATTTCCATCCTTGGCTTTAGAGAGGACTTGACACAGACCCGAGGAGCCACAACAGAATCGATTAATGCATTGCCAACTTATAAGTTTAAGCTGAAGAAAAGTAGAAGTGGAGATGATAGAGAAAACAACTCCGGGGCTGGTGAAGGAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGA

mRNA sequence

ATGGATGTTCCCTCGGTGGAACTAAATTGTGAAAGCGAAGAAGACTCTTACCCTTTATTAATGGCCCAGCCAGAAAACATCAGCAGCAGTGAGCATATCATTAACATAACTGGTACTGGTGATTCTGCTCCCTCTAGTTTGCCCCATGGTAGGAATTCAAATGGCTTAAATTCATCACAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCTTCGTCTCAACCTTCAATTTCTACTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATTCGCGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTGCTACTCACTATGAGCCAGATTATTGCAGCTATTATAGTATTATCATTGTCAAAGAATGAAAAACCTCGTGCACCTTTGTTTGCATGGATTGTGGGATATGCATCTGGGTGTGGTGCGACACTTCCTCTTCTCTATTGGCGTTATCGACACCGTAATCAGGCTTCAGAACAAGATTCTCTTCAACCTAGTCAGAATTCTAATCGCATTAATGTTCCTGCTGGGCCATTCTCTGTTTCAGTTTCTAGGGCATCAGAAGGCGAAGAACTTCATCATCCTGCCCCATCCCCCAGAGGCAGTGTAGGGTCAGGATTACTGACAGCAAGGGTAAAAGTTTTGGTTGAATATTTTAAGATGGGCCTGGATTGCTTCTTTGCTATTTGGTTTGTAGTTGGTAACGTTTGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAATTTGTACAGGCTATGTATAGTGTTTCTTACCTTTAGCTGTATTGGTTATGCCATGCCCTTCATTCTATGTGTAACCATATGCTGTTGCCTACCTTGTATAATTTCCATCCTTGGCTTTAGAGAGGACTTGACACAGACCCGAGGAGCCACAACAGAATCGATTAATGCATTGCCAACTTATAAGTTTAAGCTGAAGAAAAGTAGAAGTGGAGATGATAGAGAAAACAACTCCGGGGCTGGTGAAGGAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGA

Coding sequence (CDS)

ATGGATGTTCCCTCGGTGGAACTAAATTGTGAAAGCGAAGAAGACTCTTACCCTTTATTAATGGCCCAGCCAGAAAACATCAGCAGCAGTGAGCATATCATTAACATAACTGGTACTGGTGATTCTGCTCCCTCTAGTTTGCCCCATGGTAGGAATTCAAATGGCTTAAATTCATCACAGCCTGAAGATAGACCTTCAAGTAGTACACGAGTTCCTTCGTCTCAACCTTCAATTTCTACTACTGGATCAAATTCTAGAAATTCTTCATTCATAAGAAGAGGAGATTCGCGACGTCGTAGAAGTCCATTAAATTCTGGCTTATGGATATCTATTGAATTGCTACTCACTATGAGCCAGATTATTGCAGCTATTATAGTATTATCATTGTCAAAGAATGAAAAACCTCGTGCACCTTTGTTTGCATGGATTGTGGGATATGCATCTGGGTGTGGTGCGACACTTCCTCTTCTCTATTGGCGTTATCGACACCGTAATCAGGCTTCAGAACAAGATTCTCTTCAACCTAGTCAGAATTCTAATCGCATTAATGTTCCTGCTGGGCCATTCTCTGTTTCAGTTTCTAGGGCATCAGAAGGCGAAGAACTTCATCATCCTGCCCCATCCCCCAGAGGCAGTGTAGGGTCAGGATTACTGACAGCAAGGGTAAAAGTTTTGGTTGAATATTTTAAGATGGGCCTGGATTGCTTCTTTGCTATTTGGTTTGTAGTTGGTAACGTTTGGATCTTTGGAGGGCATTCATCAGCCTCTGAGGCTCCTAATTTGTACAGGCTATGTATAGTGTTTCTTACCTTTAGCTGTATTGGTTATGCCATGCCCTTCATTCTATGTGTAACCATATGCTGTTGCCTACCTTGTATAATTTCCATCCTTGGCTTTAGAGAGGACTTGACACAGACCCGAGGAGCCACAACAGAATCGATTAATGCATTGCCAACTTATAAGTTTAAGCTGAAGAAAAGTAGAAGTGGAGATGATAGAGAAAACAACTCCGGGGCTGGTGAAGGAGGAGGAGGGGTTGTGGCTGCTGGAACTGAAAAGGAGCGA

Protein sequence

MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQPEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNRINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIWFVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Homology
BLAST of Cp4.1LG07g02730 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 8.3e-18
Identity = 73/251 (29.08%), Postives = 114/251 (45.42%), Query Frame = 0

Query: 109 ISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQAS 168
           + ++ +  ++ ++ A  VL LS +E P  PL  WI+GY   C   +  +   YR RN   
Sbjct: 75  VVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRR 134

Query: 169 EQDSLQPSQNSNRINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEY 228
            +D L P  +S+         S S S   E E L     S    +  G L        ++
Sbjct: 135 RRD-LSPRSSSSS--------SSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKH 194

Query: 229 FKMGLDCFFAIWFVVGNVWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGYAMPFI 288
            +        IW+V+G  W+  GG   A  +P LY LCIVFL F       C+  A+  +
Sbjct: 195 LESANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCV--ALACV 254

Query: 289 LCVTICCCLPCIISILGFREDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGE 348
           + + +CCCLPCII++L     + +  GA+ E I+ L  +KF+          +   G G+
Sbjct: 255 IGIAVCCCLPCIIAVL---YAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGD 311

Query: 349 GGGGVVAAGTE 353
            GG +   GT+
Sbjct: 315 SGGVMTECGTD 311

BLAST of Cp4.1LG07g02730 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.5e-16
Identity = 71/256 (27.73%), Postives = 116/256 (45.31%), Query Frame = 0

Query: 109 ISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQAS 168
           + ++++  ++ +  A  +L +S+ E P  PL  W++GYA  C   +  +   YR RN+  
Sbjct: 101 VVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYRRRNR-- 160

Query: 169 EQDSLQPSQNSNRINVPAGPFSVSVSRASEGEELHHPAPSPRGSVG-----SGLLTARVK 228
                   + +NR      P S S S +S    L   A   R + G      G L     
Sbjct: 161 --------RRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESS 220

Query: 229 VLVEYFKMGLDCFFAIWFVVGNVWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGY 288
            + ++ +     F  IW+++G  W+  GG   A E+P +Y L IVFL F       C+  
Sbjct: 221 SVAKHLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCV-- 280

Query: 289 AMPFILCVTICCCLPCIISILGFREDLTQTRGATTESINALPTYKFKLKKSRSGDDRENN 348
           A+  ++ + +CCCLPCII++L     +    GA+ E I  L   KFK +K    +   N+
Sbjct: 281 ALACVIGIAVCCCLPCIIAVL---YAVADQEGASKEDIEQLT--KFKFRKLGDANKHTND 339

Query: 349 SGAGEGGGGVVAAGTE 353
              G   G +   GT+
Sbjct: 341 EAQGTTEGIMTECGTD 339

BLAST of Cp4.1LG07g02730 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.0e-12
Identity = 84/328 (25.61%), Postives = 141/328 (42.99%), Query Frame = 0

Query: 28  SSSEHIINITGTGDSAPSSLPHGR-NSNGLNSSQPEDRPSSSTRVPSSQPSISTTGSNSR 87
           SSS    N T   DS+      GR NS+G+  + P   P+ +  +   + S     S  R
Sbjct: 3   SSSSTTTNTTTESDSSSLPTHIGRSNSDGIIDTTPFLPPTVTRTISVDEESNPIHRSARR 62

Query: 88  N-----SSFIRRGDSRR-RRSPL-------------NSGLW------ISIELLLTMSQII 147
                 + F+R   SRR  R P                  W      + +++L  ++ + 
Sbjct: 63  QGLREAARFLRHAGSRRMMREPSMLVRETAAEQLEERQSDWAYSKPVVFLDILWNLAFVA 122

Query: 148 AAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNR 207
             + VL LS++EKP  PL  W+VGY   C   +  +   YR R +    +    S  +N 
Sbjct: 123 IGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPEDGGGSGLTN- 182

Query: 208 INVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIWF 267
               +    VS+++  +  E  +PA                    ++ +     F  IW+
Sbjct: 183 ---SSSQQYVSLAQLEDRGETSNPA--------------------KHLESANTMFSFIWW 242

Query: 268 VVGNVWI-FGGHSSASEAPNLYRLCIVFLTFS------CIGYAMPFILCVTICCCLPCII 323
           ++G  W+  GG + +S++P LY LCI+FL F       C+  A+  ++ + +CCCLPCII
Sbjct: 243 IIGFYWVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCV--ALACVIGLAVCCCLPCII 301

BLAST of Cp4.1LG07g02730 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.4e-09
Identity = 57/240 (23.75%), Postives = 103/240 (42.92%), Query Frame = 0

Query: 109 ISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQAS 168
           ++ ++L   + ++A+ ++L  +  E+P  P+  WI  Y   C   + L++  Y  RN   
Sbjct: 78  VAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEYWRRNSTR 137

Query: 169 EQDSLQPSQNSNRINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLL--TARVKVLV 228
               L+ S +    N+                E  +   S   S     +     +  ++
Sbjct: 138 RARDLE-SYDHEDYNI----------------EYDYEQDSDDNSTTYSFVKRCESINTVI 197

Query: 229 EYFKMGLDCFFAIWFVVGNVWIF-GGHSSASEAPNLYRLCIVFLT----FSCIGYAMPFI 288
            +          IW+++G  W+  GG     EAPNLY L ++FL     F+     +  +
Sbjct: 198 SF----------IWWIIGFYWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACL 257

Query: 289 LCVTICCCLPCIISILGFREDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGE 342
           + + +CCCLPCII++L     +  T G +   +  LP YKFK   S    + +N +G G+
Sbjct: 258 VGIALCCCLPCIIALL---YAVAGTEGVSEAELGVLPLYKFKAFHS----NEKNITGPGK 283

BLAST of Cp4.1LG07g02730 vs. NCBI nr
Match: XP_023537060.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo] >XP_023537061.1 E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692 bits (1786), Expect = 8.20e-250
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. NCBI nr
Match: KAG6585434.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020355.1 E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691 bits (1784), Expect = 1.65e-249
Identity = 354/355 (99.72%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           ED+TQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDMTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. NCBI nr
Match: XP_023537851.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689 bits (1778), Expect = 2.84e-249
Identity = 353/354 (99.72%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKE 354
           EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEK+
Sbjct: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKD 354

BLAST of Cp4.1LG07g02730 vs. NCBI nr
Match: XP_022951236.1 (E3 ubiquitin-protein ligase At1g63170 [Cucurbita moschata] >XP_022951237.1 E3 ubiquitin-protein ligase At1g63170 [Cucurbita moschata])

HSP 1 Score: 688 bits (1776), Expect = 2.74e-248
Identity = 352/355 (99.15%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSS RVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSARVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGAT+PLLYWRYRHRNQASEQDSLQPSQNSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATVPLLYWRYRHRNQASEQDSLQPSQNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           ED+TQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDMTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. NCBI nr
Match: XP_023002356.1 (E3 ubiquitin-protein ligase At1g63170 [Cucurbita maxima] >XP_023002357.1 E3 ubiquitin-protein ligase At1g63170 [Cucurbita maxima])

HSP 1 Score: 685 bits (1767), Expect = 6.43e-247
Identity = 352/355 (99.15%), Postives = 352/355 (99.15%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPS NSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSHNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPS RGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSTRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDLTQTRGATTESINALPTYKFK KKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDLTQTRGATTESINALPTYKFKQKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. ExPASy TrEMBL
Match: A0A6J1GH37 (E3 ubiquitin-protein ligase At1g63170 OS=Cucurbita moschata OX=3662 GN=LOC111454136 PE=4 SV=1)

HSP 1 Score: 688 bits (1776), Expect = 1.33e-248
Identity = 352/355 (99.15%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSS RVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSARVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGAT+PLLYWRYRHRNQASEQDSLQPSQNSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATVPLLYWRYRHRNQASEQDSLQPSQNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           ED+TQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDMTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. ExPASy TrEMBL
Match: A0A6J1KJB2 (E3 ubiquitin-protein ligase At1g63170 OS=Cucurbita maxima OX=3661 GN=LOC111496220 PE=4 SV=1)

HSP 1 Score: 685 bits (1767), Expect = 3.11e-247
Identity = 352/355 (99.15%), Postives = 352/355 (99.15%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPS NSN
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSHNSN 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFSVSVSRASEGEELHHPAPS RGSVGSGLLTARVKVLVEYFKMGLDCFFAIW
Sbjct: 181 RINVPAGPFSVSVSRASEGEELHHPAPSTRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDLTQTRGATTESINALPTYKFK KKSRSGDDRENNSGAGEGGGGVVAAGTEKER
Sbjct: 301 EDLTQTRGATTESINALPTYKFKQKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355

BLAST of Cp4.1LG07g02730 vs. ExPASy TrEMBL
Match: A0A0A0LPE6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379250 PE=4 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 5.48e-229
Identity = 327/355 (92.11%), Postives = 344/355 (96.90%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNC+SE DSYPLLMA+PE+ +SSEHII+ITG GDS PS LPHGR+SNGLNSSQ
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSGPSGLPHGRSSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVP SQPS S+TGSNSRNSSFIRRGD+RRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPLSQPSTSSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQ SQ+S+
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQSSS 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFS+SVSRASEGEEL HPAPSPRGS GSG+L+AR+KVLVEYFKMGLDCFFA+W
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDL+QTRGAT+ESINALPTYKFKLKKSRSGDDRENNSGAGEGGG VVAAGTEKER
Sbjct: 301 EDLSQTRGATSESINALPTYKFKLKKSRSGDDRENNSGAGEGGG-VVAAGTEKER 354

BLAST of Cp4.1LG07g02730 vs. ExPASy TrEMBL
Match: A0A6J1BPC9 (E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC111004615 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 7.78e-229
Identity = 329/355 (92.68%), Postives = 343/355 (96.62%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNCESEEDSYPLLMA+PEN  SSEH+I+ITGTGDS+ SSLPHGRNSNGLNSSQ
Sbjct: 1   MDVPSVELNCESEEDSYPLLMARPENFDSSEHVIDITGTGDSS-SSLPHGRNSNGLNSSQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSSSTRVP SQPSIS+TGSNSRNSSFIRRGD+RRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSSTRVPMSQPSISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLS++EKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQ SQNS+
Sbjct: 121 IAAIIVLSLSRDEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFS+SVSR SEGEEL HPA SPRGS  SG+ TAR+KV+VEYFKMGLDCFFA+W
Sbjct: 181 RINVPAGPFSLSVSRTSEGEELQHPAQSPRGSQVSGVFTARLKVMVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGG VVAAGTEKER
Sbjct: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGG-VVAAGTEKER 353

BLAST of Cp4.1LG07g02730 vs. ExPASy TrEMBL
Match: A0A1S3BBX9 (E3 ubiquitin-protein ligase At1g63170 OS=Cucumis melo OX=3656 GN=LOC103488256 PE=4 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 3.68e-227
Identity = 326/355 (91.83%), Postives = 342/355 (96.34%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQ 60
           MDVPSVELNC+SE DSYPLLMA+PE+ +SSEHII+ITG GDSA SSLP GR+SNGLNS Q
Sbjct: 1   MDVPSVELNCDSEGDSYPLLMARPEHSNSSEHIIDITGAGDSASSSLPRGRSSNGLNSLQ 60

Query: 61  PEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQI 120
           PEDRPSS+TRVP SQP IS+TGSNSRNSSFIRRGD+RRRRSPLNSGLWISIELLLTMSQI
Sbjct: 61  PEDRPSSNTRVPLSQPPISSTGSNSRNSSFIRRGDARRRRSPLNSGLWISIELLLTMSQI 120

Query: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSN 180
           IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQ SQNS+
Sbjct: 121 IAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQSSQNSS 180

Query: 181 RINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIW 240
           RINVPAGPFS+SVSRASEGEEL HPAPSPRGS GSG+L+AR+KVLVEYFKMGLDCFFA+W
Sbjct: 181 RINVPAGPFSLSVSRASEGEELQHPAPSPRGSQGSGVLSARLKVLVEYFKMGLDCFFAVW 240

Query: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300
           FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR
Sbjct: 241 FVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFR 300

Query: 301 EDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 355
           EDLTQTRGAT+ESINALPTYKFKLKKSRSGDDRENNSG GEGGG VVAAGTEKER
Sbjct: 301 EDLTQTRGATSESINALPTYKFKLKKSRSGDDRENNSGTGEGGG-VVAAGTEKER 354

BLAST of Cp4.1LG07g02730 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 9.8e-107
Identity = 224/349 (64.18%), Postives = 261/349 (74.79%), Query Frame = 0

Query: 12  SEEDSYPLLMAQ-PE-NISSSEH-IINITGTGDSAPSSLPHGRNSNGLNSSQ-PEDRPSS 71
           ++ D++   M + PE N+S +EH II+I      A SS  H R SN L   Q  E+RPS+
Sbjct: 14  NQADTFSRFMERSPENNLSRNEHIIIDIPRNAAIASSSSSHDRISNVLEPLQHEEERPST 73

Query: 72  STRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWISIELLLTMSQIIAAIIVL 131
            + + + QP+ +T  S+S   S  R    RRRRSPLNSGLWISIEL LT+ QIIAAI+VL
Sbjct: 74  VSPMAAPQPATATASSSSSMRSNPR--SVRRRRSPLNSGLWISIELFLTVGQIIAAIVVL 133

Query: 132 SLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNRINVPAG 191
           SLSK+E PRAPLF WIVGYA GC ATLPLLYWRY H NQASEQDS    Q+   +NV AG
Sbjct: 134 SLSKHEHPRAPLFTWIVGYACGCVATLPLLYWRYYHSNQASEQDS---GQHRPNLNVAAG 193

Query: 192 PFSVSVSRASEGEELHHPAPSPRGSVGSGLLT-ARVKVLVEYFKMGLDCFFAIWFVVGNV 251
           PF+ S+SR SE +       S RGS   G ++ AR+KV+VEYFKM LDCFFA+WFVVGNV
Sbjct: 194 PFAFSISRTSEADGRQTNTTSSRGSRYPGFISAARLKVIVEYFKMALDCFFAVWFVVGNV 253

Query: 252 WIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDLTQT 311
           WIFGGHSSA+EAPNLYRLC+VFLTFSCIGYAMPFILC TICCCLPCIISILG+REDLTQ 
Sbjct: 254 WIFGGHSSAAEAPNLYRLCLVFLTFSCIGYAMPFILCTTICCCLPCIISILGYREDLTQP 313

Query: 312 RGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAAGTEKER 356
           RGAT ESINALPT+KFKLKKSRS  D   ++G+    GGVVAAGT+ ER
Sbjct: 314 RGATPESINALPTHKFKLKKSRSNGD---DNGSSTSEGGVVAAGTDNER 354

BLAST of Cp4.1LG07g02730 vs. TAIR 10
Match: AT1G80400.1 (RING/U-box superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.5e-83
Identity = 179/366 (48.91%), Postives = 240/366 (65.57%), Query Frame = 0

Query: 1   MDVPSVELNCESEEDSYPLLMAQPENISSSEHIINITGTGDSAPSS-------LPHGRNS 60
           MDV S+  N  +  D  PLLM    +  + EHI++IT   +   SS        P G + 
Sbjct: 1   MDVSSIVRN-TTLTDQDPLLM----DHGNREHIVDITSNDEEDSSSRSSLDELTPQGNSH 60

Query: 61  NG----LNSSQPEDRPSSSTRVPSSQPSISTTGSNSRNSSFIRRGDSRRRRSPLNSGLWI 120
            G    L+SS    +  +    P  +P+ S  G N         G+ RR RSPLNSGLWI
Sbjct: 61  QGEETVLSSSNATQQHPTHPSPPQQRPTSSRRGDN---------GNGRRTRSPLNSGLWI 120

Query: 121 SIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAWIVGYASGCGATLPLLYWRYRHRNQASE 180
           S+EL++T++QI+AAI+V+ L+K+E P APLF W++GY  GC ATLP+LYWR+R  N+A+ 
Sbjct: 121 SVELVVTVAQIVAAIVVMVLAKDEHPEAPLFTWVIGYTCGCLATLPILYWRFRTYNRATG 180

Query: 181 QDSLQPSQNSNRINVPAGPFSVSVSRASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYF 240
           QDS Q + +S   +      +VSV++A++ E     + +PR +     L  R+  LV++F
Sbjct: 181 QDSSQRATSSQGNSESTPYTAVSVAQAADEENSTGVSAAPRNNQVGESLRTRLNGLVDHF 240

Query: 241 KMGLDCFFAIWFVVGNVWIFGGHSSASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCC 300
           KM +DCFFA+WFVVGNVWIFGGHSS S++P LYRLCI FLTFSCIGYAMPFILC TICCC
Sbjct: 241 KMAIDCFFAVWFVVGNVWIFGGHSSPSDSPKLYRLCIAFLTFSCIGYAMPFILCATICCC 300

Query: 301 LPCIISILGFREDLTQTRGATTESINALPTYKFKLKKSRSGDDRENNSGAGEGGGGVVAA 356
           LPC+IS+LGFRE+ +QTRGAT E+INALP Y+F   KS+S +D E +    EG GG +  
Sbjct: 301 LPCLISVLGFRENFSQTRGATAEAINALPVYRF---KSKSRNDLEFSE---EGEGGFLLL 346

BLAST of Cp4.1LG07g02730 vs. TAIR 10
Match: AT4G26580.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.2e-25
Identity = 98/316 (31.01%), Postives = 149/316 (47.15%), Query Frame = 0

Query: 30  SEHIINITGTGDSAPSSLPHGRNSNGLNSSQPEDRPSSSTRVPSSQPSISTTGSNSRNSS 89
           S  I +IT +  S+ +S     N++    + PE  PSSS  +      ++   S +R   
Sbjct: 11  SSAINSITVSSSSSFASTQLDDNTH----TAPESSPSSSFFM-----RLAMRVSRARWFI 70

Query: 90  FIRR-------GDSRRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAW 149
           F+RR         S    +P NS  W+  EL+  + QI      L+LSKNE+P  P+  W
Sbjct: 71  FLRRVFHYQNASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLW 130

Query: 150 IVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNRINVPAGPFSVSVSRASEGEEL 209
           I GY  GC   L LLY RYR ++ + E                A  F     +    EE 
Sbjct: 131 ITGYNVGCLLNLMLLYGRYRQQDTSHEN---------------AFSFGDIEQQQRSREE- 190

Query: 210 HHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIWFVVGNVWIFGGH-SSASEAPNL 269
                           T R   L+   +  L+ FFAIWFV+GNVW+F     S   AP L
Sbjct: 191 ----------------TTRCSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHYAPIL 250

Query: 270 YRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDL-TQTRGATTESINALPTY 329
           + LCI  L ++ + Y+ PF+L + +CC +P + S LG+  ++ +  +GA+ + I++LP++
Sbjct: 251 HVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGYNMNVGSSEKGASDDQISSLPSW 285

Query: 330 KFKLKKSRSGDDRENN 337
           K+KL    S   + NN
Sbjct: 311 KYKLIDETSDSSQANN 285

BLAST of Cp4.1LG07g02730 vs. TAIR 10
Match: AT4G26580.2 (RING/U-box superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.2e-25
Identity = 98/316 (31.01%), Postives = 149/316 (47.15%), Query Frame = 0

Query: 30  SEHIINITGTGDSAPSSLPHGRNSNGLNSSQPEDRPSSSTRVPSSQPSISTTGSNSRNSS 89
           S  I +IT +  S+ +S     N++    + PE  PSSS  +      ++   S +R   
Sbjct: 11  SSAINSITVSSSSSFASTQLDDNTH----TAPESSPSSSFFM-----RLAMRVSRARWFI 70

Query: 90  FIRR-------GDSRRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKPRAPLFAW 149
           F+RR         S    +P NS  W+  EL+  + QI      L+LSKNE+P  P+  W
Sbjct: 71  FLRRVFHYQNASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLW 130

Query: 150 IVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNRINVPAGPFSVSVSRASEGEEL 209
           I GY  GC   L LLY RYR ++ + E                A  F     +    EE 
Sbjct: 131 ITGYNVGCLLNLMLLYGRYRQQDTSHEN---------------AFSFGDIEQQQRSREE- 190

Query: 210 HHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIWFVVGNVWIFGGH-SSASEAPNL 269
                           T R   L+   +  L+ FFAIWFV+GNVW+F     S   AP L
Sbjct: 191 ----------------TTRCSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGSFHYAPIL 250

Query: 270 YRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDL-TQTRGATTESINALPTY 329
           + LCI  L ++ + Y+ PF+L + +CC +P + S LG+  ++ +  +GA+ + I++LP++
Sbjct: 251 HVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGYNMNVGSSEKGASDDQISSLPSW 285

Query: 330 KFKLKKSRSGDDRENN 337
           K+KL    S   + NN
Sbjct: 311 KYKLIDETSDSSQANN 285

BLAST of Cp4.1LG07g02730 vs. TAIR 10
Match: AT5G55970.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.2e-25
Identity = 101/323 (31.27%), Postives = 150/323 (46.44%), Query Frame = 0

Query: 23  QPENISSSEHIINITGTGDSAPSSLPHGRNSNGLNSSQPEDRPSSSTRVPSSQPSISTTG 82
           QPE  SS+   I IT +  +  SS P G NS+   ++  E  PS      S    ++   
Sbjct: 8   QPELSSSN---ITITISSSALLSSSPRGDNSHVAAANGQERSPS------SFYIRLAMKV 67

Query: 83  SNSRNSSFIRR-------GDSRRRRSPLNSGLWISIELLLTMSQIIAAIIVLSLSKNEKP 142
           S +R   F+RR         S    +P NS  W+  EL+  + Q+      L++SK E+P
Sbjct: 68  SRARWFIFLRRVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVITFTLAISKEERP 127

Query: 143 RAPLFAWIVGYASGCGATLPLLYWRYRHRNQASEQDSLQPSQNSNRINVPAGPFSVSVSR 202
             P+  WI GY  GC   L LLY RYR  +                IN   G     V +
Sbjct: 128 IWPVRLWITGYDVGCLLNLMLLYGRYRQLD----------------INQGNGFVLGDVEQ 187

Query: 203 ASEGEELHHPAPSPRGSVGSGLLTARVKVLVEYFKMGLDCFFAIWFVVGNVWIFGGH-SS 262
              G E                   R   L+   +  L+ FFAIWFV+GNVW+F     S
Sbjct: 188 QQRGRE-----------------ETRSSHLMNKCRTSLELFFAIWFVIGNVWVFDSRFGS 247

Query: 263 ASEAPNLYRLCIVFLTFSCIGYAMPFILCVTICCCLPCIISILGFREDL-TQTRGATTES 322
              AP L+ LC+  L ++ I Y+ PF+L + +CC +P I S+LG+  ++ +  R A+ + 
Sbjct: 248 FHHAPKLHVLCVSLLAWNAICYSFPFLLFLFLCCLVPLISSLLGYNMNMGSSDRAASDDQ 288

Query: 323 INALPTYKFK-LKKSRSGDDREN 336
           I++LP++KFK +  S S  D ++
Sbjct: 308 ISSLPSWKFKRIDDSASDSDSDS 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LDB88.3e-1829.08E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q9LN713.5e-1627.73E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q93Z924.0e-1225.61E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8GUU22.4e-0923.75E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_023537060.18.20e-250100.00E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6585434.11.65e-24999.72E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia] >KA... [more]
XP_023537851.12.84e-24999.72E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo][more]
XP_022951236.12.74e-24899.15E3 ubiquitin-protein ligase At1g63170 [Cucurbita moschata] >XP_022951237.1 E3 ub... [more]
XP_023002356.16.43e-24799.15E3 ubiquitin-protein ligase At1g63170 [Cucurbita maxima] >XP_023002357.1 E3 ubiq... [more]
Match NameE-valueIdentityDescription
A0A6J1GH371.33e-24899.15E3 ubiquitin-protein ligase At1g63170 OS=Cucurbita moschata OX=3662 GN=LOC111454... [more]
A0A6J1KJB23.11e-24799.15E3 ubiquitin-protein ligase At1g63170 OS=Cucurbita maxima OX=3661 GN=LOC11149622... [more]
A0A0A0LPE65.48e-22992.11RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379250 P... [more]
A0A6J1BPC97.78e-22992.68E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A1S3BBX93.68e-22791.83E3 ubiquitin-protein ligase At1g63170 OS=Cucumis melo OX=3656 GN=LOC103488256 PE... [more]
Match NameE-valueIdentityDescription
AT4G32600.19.8e-10764.18RING/U-box superfamily protein [more]
AT1G80400.11.5e-8348.91RING/U-box superfamily protein [more]
AT4G26580.14.2e-2531.01RING/U-box superfamily protein [more]
AT4G26580.24.2e-2531.01RING/U-box superfamily protein [more]
AT5G55970.14.2e-2531.27RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 325..355
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..90
NoneNo IPR availablePANTHERPTHR46225C3H4 TYPE ZINC FINGER PROTEINcoord: 1..355
NoneNo IPR availablePANTHERPTHR46225:SF2C3H4 TYPE ZINC FINGER PROTEINcoord: 1..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g02730.1Cp4.1LG07g02730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008270 zinc ion binding