Cp4.1LG05g10220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g10220
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	26S proteasome regulatory subunit 6B homolog
Location	Cp4.1LG05: 6601840 .. 6602242 (+)
RNA-Seq Expression	Cp4.1LG05g10220
Synteny	Cp4.1LG05g10220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATGGAGTGTAGGCCAGAGATGGTCATTGTTCGTGGCTTTCTGGGATTAGTTAAGTAAAGAGATTTAGAAAGGAATGCTTGTCTGAAGAAATTCCATGCTCCTCCTTAATTGTTAAAAATGCTGTTACAGTCTTCCTTTCATGTAGGAGCATTTAGTTGGCATCTAGCGTTGAATTAGATTACAAAAACAAATTTTGTGTATCATTTTCCCCTCTTCTTTATGGAGTTCAACATTAATCAATAGTTGTGATGGCCAACTGCAGATCGCGGCCATCTGTCAAGAAGCTGGGATGCACGCGGTTCGTAAGAACAGATATGTGATACTCCCGAAGGATTTCGAGAAGGGATACAGAACCAACGTGAAGAAGCCTGATACCGACTTTGAATTCTACAAATGA

mRNA sequence

ATGGGAATGGAGTGTAGGCCAGAGATGGTCATTGTTCGTGGCTTTCTGGGATTAGTTAATTGTGATGGCCAACTGCAGATCGCGGCCATCTGTCAAGAAGCTGGGATGCACGCGGTTCGTAAGAACAGATATGTGATACTCCCGAAGGATTTCGAGAAGGGATACAGAACCAACGTGAAGAAGCCTGATACCGACTTTGAATTCTACAAATGA

Coding sequence (CDS)

ATGGGAATGGAGTGTAGGCCAGAGATGGTCATTGTTCGTGGCTTTCTGGGATTAGTTAATTGTGATGGCCAACTGCAGATCGCGGCCATCTGTCAAGAAGCTGGGATGCACGCGGTTCGTAAGAACAGATATGTGATACTCCCGAAGGATTTCGAGAAGGGATACAGAACCAACGTGAAGAAGCCTGATACCGACTTTGAATTCTACAAATGA

Protein sequence

MGMECRPEMVIVRGFLGLVNCDGQLQIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK

Homology

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot
Match: P54778 (26S proteasome regulatory subunit 6B homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 5.6e-19
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = 0


Query: 26  QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 71
           +I AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 369 EITAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 413

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot
Match: Q9SEI4 (26S proteasome regulatory subunit 6B homolog OS=Arabidopsis thaliana OX=3702 GN=RPT3 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 7.3e-19
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = 0


Query: 26  QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 71
           +IAAICQEAGMHAVRKNRYVILPKDFEKGYR NVKKPDTDFEFYK
Sbjct: 364 EIAAICQEAGMHAVRKNRYVILPKDFEKGYRANVKKPDTDFEFYK 408

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot
Match: P85200 (26S proteasome regulatory subunit 6B homolog OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-18
Identity = 42/45 (93.33%), Postives = 44/45 (97.78%), Query Frame = 0


Query: 26  QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 71
           +I AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDF+FYK
Sbjct: 370 EITAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFDFYK 414

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot
Match: P46507 (26S proteasome regulatory subunit 6B OS=Manduca sexta OX=7130 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.7e-13
Identity = 31/44 (70.45%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 27  IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 71
           I AICQEAGM+AVR+NRY++LPKDFEKGY+ N+KK ++++EFYK
Sbjct: 372 INAICQEAGMNAVRENRYIVLPKDFEKGYKNNIKKDESEYEFYK 415

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot
Match: P34123 (26S proteasome regulatory subunit 6B homolog OS=Dictyostelium discoideum OX=44689 GN=psmC4 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-12
Identity = 31/44 (70.45%), Postives = 38/44 (86.36%), Query Frame = 0


Query: 26  QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFY 70
           +I +ICQEAGMHA+RKNRYVILPKDFEKGY+ ++KK   +F FY
Sbjct: 359 EIQSICQEAGMHAIRKNRYVILPKDFEKGYKASIKKNTHEFNFY 402

[Close]

BLAST of Cp4.1LG05g10220 vs. NCBI nr
Match: DAD38580.1 (TPA_asm: hypothetical protein HUJ06_012902 [Nelumbo nucifera])

HSP 1 Score: 98.2 bits (243), Expect = 3.37e-25
Identity = 46/47 (97.87%), Postives = 46/47 (97.87%), Query Frame = 0


Query: 24 QLQIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          QLQIAAICQEAGMHAVRKNRYVILPKDFEKGYR NVKKPDTDFEFYK
Sbjct: 12 QLQIAAICQEAGMHAVRKNRYVILPKDFEKGYRANVKKPDTDFEFYK 58

[Close]

BLAST of Cp4.1LG05g10220 vs. NCBI nr
Match: KAF2560627.1 (hypothetical protein F2Q70_00014793 [Brassica cretica])

HSP 1 Score: 95.1 bits (235), Expect = 6.70e-24
Identity = 44/45 (97.78%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          QIAAICQEAGMHAVRKNRYVILPKDFEKGYR+NVKKPDTDFEFYK
Sbjct: 21 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRSNVKKPDTDFEFYK 65

[Close]

BLAST of Cp4.1LG05g10220 vs. NCBI nr
Match: RHN67943.1 (putative proteasome ATPase [Medicago truncatula])

HSP 1 Score: 94.4 bits (233), Expect = 1.43e-23
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +I+AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 23 EISAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 67

[Close]

BLAST of Cp4.1LG05g10220 vs. NCBI nr
Match: OIV91967.1 (hypothetical protein TanjilG_26489 [Lupinus angustifolius])

HSP 1 Score: 94.4 bits (233), Expect = 1.43e-23
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +I+AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 23 EISAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 67

[Close]

BLAST of Cp4.1LG05g10220 vs. NCBI nr
Match: ACA58350.1 (putative 26S proteasome regulatory complex protein, partial [Sandersonia aurantiaca])

HSP 1 Score: 94.4 bits (233), Expect = 1.59e-23
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDF+FYK
Sbjct: 27 EIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFDFYK 71

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL
Match: A0A1J7FVH8 (AAA_lid_3 domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_26489 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 6.90e-24
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +I+AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 23 EISAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 67

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL
Match: A0A396ITY6 (Putative proteasome ATPase OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr3g0108311 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 6.90e-24
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +I+AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 23 EISAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 67

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL
Match: B1PJP1 (Putative 26S proteasome regulatory complex protein (Fragment) OS=Sandersonia aurantiaca OX=61864 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 7.68e-24
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 0


Query: 26 QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          +IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDF+FYK
Sbjct: 27 EIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFDFYK 71

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL
Match: A0A2K3LJU0 (26S protease regulatory subunit 6b (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g034737 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 7.98e-24
Identity = 43/44 (97.73%), Postives = 44/44 (100.00%), Query Frame = 0


Query: 27 IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          I+AICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK
Sbjct: 1  ISAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 44

[Close]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL
Match: A0A5K1DEU7 (Uncharacterized protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS19486 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 7.98e-24
Identity = 43/44 (97.73%), Postives = 44/44 (100.00%), Query Frame = 0


Query: 27 IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 70
          IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDF+FYK
Sbjct: 1  IAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFDFYK 44

[Close]

BLAST of Cp4.1LG05g10220 vs. TAIR 10
Match: AT5G58290.1 (regulatory particle triple-A ATPase 3 )

HSP 1 Score: 94.0 bits (232), Expect = 5.2e-20
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = 0


Query: 26  QIAAICQEAGMHAVRKNRYVILPKDFEKGYRTNVKKPDTDFEFYK 71
           +IAAICQEAGMHAVRKNRYVILPKDFEKGYR NVKKPDTDFEFYK
Sbjct: 364 EIAAICQEAGMHAVRKNRYVILPKDFEKGYRANVKKPDTDFEFYK 408

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g10220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P54778	5.6e-19	95.56	26S proteasome regulatory subunit 6B homolog OS=Solanum tuberosum OX=4113 PE=2 S...	[more]
Q9SEI4	7.3e-19	95.56	26S proteasome regulatory subunit 6B homolog OS=Arabidopsis thaliana OX=3702 GN=...	[more]
P85200	1.3e-18	93.33	26S proteasome regulatory subunit 6B homolog OS=Helianthus annuus OX=4232 PE=1 S...	[more]
P46507	2.7e-13	70.45	26S proteasome regulatory subunit 6B OS=Manduca sexta OX=7130 PE=2 SV=1	[more]
P34123	1.3e-12	70.45	26S proteasome regulatory subunit 6B homolog OS=Dictyostelium discoideum OX=4468...	[more]

BLAST of Cp4.1LG05g10220 vs. NCBI nr


Match Name	E-value	Identity	Description
DAD38580.1	3.37e-25	97.87	TPA_asm: hypothetical protein HUJ06_012902 [Nelumbo nucifera]	[more]
KAF2560627.1	6.70e-24	97.78	hypothetical protein F2Q70_00014793 [Brassica cretica]	[more]
RHN67943.1	1.43e-23	95.56	putative proteasome ATPase [Medicago truncatula]	[more]
OIV91967.1	1.43e-23	95.56	hypothetical protein TanjilG_26489 [Lupinus angustifolius]	[more]
ACA58350.1	1.59e-23	95.56	putative 26S proteasome regulatory complex protein, partial [Sandersonia auranti...	[more]

BLAST of Cp4.1LG05g10220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1J7FVH8	6.90e-24	95.56	AAA_lid_3 domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_...	[more]
A0A396ITY6	6.90e-24	95.56	Putative proteasome ATPase OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr3g01083...	[more]
B1PJP1	7.68e-24	95.56	Putative 26S proteasome regulatory complex protein (Fragment) OS=Sandersonia aur...	[more]
A0A2K3LJU0	7.98e-24	97.73	26S protease regulatory subunit 6b (Fragment) OS=Trifolium pratense OX=57577 GN=...	[more]
A0A5K1DEU7	7.98e-24	97.73	Uncharacterized protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS19...	[more]

BLAST of Cp4.1LG05g10220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58290.1	5.2e-20	95.56	regulatory particle triple-A ATPase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.8.60		coord: 17..70 e-value: 1.5E-10 score: 42.8
None	No IPR available	PANTHER	PTHR23073:SF96	BNAC09G33780D PROTEIN	coord: 26..70
None	No IPR available	PANTHER	PTHR23073	26S PROTEASOME REGULATORY SUBUNIT	coord: 26..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g10220.1	Cp4.1LG05g10220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901800	positive regulation of proteasomal protein catabolic process
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0008540	proteasome regulatory particle, base subcomplex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0036402	proteasome-activating activity