Cp4.1LG05g07260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g07260
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG05: 4483697 .. 4487410 (+)
RNA-Seq Expression	Cp4.1LG05g07260
Synteny	Cp4.1LG05g07260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCATTTTACTATCAATTTCCCCATCTTTGTCAGCCCCCAACGCCAAGGAGTTATGGTGCAGCCAAACCCCATACCCCGACATCTGCAAGAGCTTCTTCAACGCCAAATTCAACCCCAAAAACCTCGCCGAGTTCAAAAAAGCCGCCTTCAAAATCGCCATGGAAAGGGCTCTCACAGCCGAGTCGCTCACCAAGCGCCACGCCCAGAAATGCCGCACCCCCAAGGAAAAAGGCGCGTGGGCCGACTGCTTGGAGCTTTACCAAGACACCATTCTCCAGCTCAACCGAACCTTCTCCGACAACTATTGCTCCGATTTCGACATCCAAACATGGCTCAGCTCCGCGTTGACTAATCTTGATACATGTCGTGCCGGATTCGTTGACCTTAATATTAAGGATTACGACGTCGTTTTTCCTTTTCTTGGGAGGAGTAATAATGTGACTAAGTTGATTAGGAATAGTTTGGCTATGAATAACTGCTCTGAGACGGAGACGGGTTATCATGGTGGATTTCCTACGTGGGTTAAGGCTGGTGAACGGCGGCTGCTGCAGGCGGCGGTTCCTAAGCCGGACTTGGTTGTGGCGCTTGATGGGTCTGGGAATTATAAGACGGTGCAGGCGGCGGTGGATGCGGCGGCGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATGTTAAGAAAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAGGTCAAGAATTTGATGCTCGTCGGAGATGGGTTGAAGTATACCATTATTACTGGGAGTAGGAGTGTCGGCGGCGGCTCCACCACGTTCAACTCCGCCACCGTCGGTACGTTTCTTCTCTCTCTCCTTTTTCTCTCACTAACCCAACTTAACCCGAAATTAGTGTTGAAAATAGTTGGGAACCAGTCCGACATTCATTAATTTAACGAATGATCATGAGTTTATAAATAAGAATATATCTACAATAATACGAGGCCTTCTGGGTAAACCCAGAGTAAAGCCATGAAAATTTAGGTTCAAAGTGAACAATATTATAAAATTGTGGAGAGTCGCGATATTTAACAAATAAATTGGTGTAAATTTTGTGACTTTATTCGTGAACTTATGCTTAAAGTGGACAATATCATATATGATTTCTAACTAATAAATTATTGTAAATTTTGAAATTCTGTCCACCGTGAGCTTATGCTCAAAGTGGACAATATCATACAATTGTAGAGAGTCGTGATTTCTAACTAATAAATTATTATAACTTTTGAAACTTTGTCCACCGTGAGCTTATGCTCAAAGTGGACAATATCATACAATTGTAGGGAGTCGTGATTTCTAACTAATAAATTATTGTAAATTTTGAAACTTTGTCCACGGTGAGCTTATGCTCAAAGTGGACAATATCATACAATCGTAGAGAGTCGTGATTTCTAACTAATAAATTATTGTAAATTTTGAAACTTTGTCCACGGTGAGCTTATGCTCAAAGTGGACAATATCATACAATCGTAGAGAGTCGTGATTTCTAACTAATAAATTATTGTAAATTTTGAAACTTTGTCCACGGTGAGCTTATGCTCAAAGTGGACAATATCATACAATCGTAGAGAGTCGTGATTTCTAACTAATAAATTATTGTAAATTTTGAAACTTTGTCCACTATGAGCTTATGCTCAAAGTCGACAATATCGTACCATTGTAGAGAGTCGTGATTTCTAACAAATAAATTATTGTAAATTTTGACAACATAGAGCTTATGCTCAAAGTGAACAATATCATACCACGGTAGAGAGTCGTGATTTATAACAAATCTGTGAGCTTATGTTTAAAGCGGACAATTTCGTACCATTACGAAGAGTCGTGATTTCTAACAAATAAATTGTTGCAAATTTTGTGACTTTGTTCAGCTGTTACTGGGGAAGGATTCATTGCTCGTGGCATCACCTTCCGCAACACGGCCGGACCTCAAAACCACCAAGCAGTAGCTCTCCGATCGGGCTCAGATCTCTCCGTCTTCTACCGCTGCGGCTTCGAGGGATATCAAGACACTCTCTACGTCCATTCCCAAAGACAATTCTACAGAGAATGCTACATTTACGGCACAGTAGACTTCATCTTCGGCAACGCAGCCGTGGTACTCCAAAACTGCATGATCTACGCGAGAAAACCAATGTCAGGTCAGCAATGCGTGGTAACAGCGCAAGGCCGATCGGACCCAAATCAGAACACCGGAATCTCAATCCACAACTCGAGAGTGATGGCCACGGCCGACCTCCAACCGGTGCTAAGCTCGGTGAGAACCTACCTGGGGAGGCCATGGAAGGAGTACTCCCGGACGGTTTACTTGCAGTGCTTCATGGATAGCTTGGTGAACCCTGCAGGGTGGCTGGAATGGAGCGGGGACTTTGCCCTGAATACGCTCTATTATGGAGAGTTCAAGAACTTTGGGCCTGGTTCGTCCACTGCTAAGCGAGTGAAGTGGAAGGGTTACAGAGTGATTACAAGCGCCGCCGAAGCTTCCAAGTTTACCGTCGGGAGCTTCATCGCCGGGAAGTCATGGCTGCCGGCAACCGGCGTTCCGTTCACTGCTGGGCTTTGAAATTAATTATGGGGGACTTGGAAGATCTTTGTTCTTGTGCTGTTCTTGGTGGTTGAATTTTACTGTATTCATCAATAATAATAATATACCATTTTTTTTGGGAATTTTCTGTTAAAAATAATGTTTATTTGAAGATTATTTTGGATTCATTAATCATTTTTTTAGTGAAGTGTTTGTGCGAAAATTTGTTTTTATATTAAAATTAATTTTTTGAGAATTTTATATCGTTGTTTATTTATTGTTTTAGTCAATGAAATTGGAATTTAGAAGGAAATATATGATAAAAGTGAAAATTGATATATTTTTTTATTAAATTAATAAAAATATAAAATACTGGTAAGCTTTCGATATTTTTAATAATACTTTTAAAAATTTTAAAATATTTTTATTGAAACTATTAATATTTTATTTTAAATATATTCTTAAATTTTAAAAATAATATTAACACCTCGACTTCTTATAAAAGTTATTATTATCCTCTATTTACTTCTCATAGATTATTCATCTCTTTAATTTTTATAGGCGAGGGTTATATTCAAATTTTGTTAAAAAGAGTGTTTTAAAAACAAATTAAAAATATACCACTTTTTAAAAAAATATTTTTTAAACATTTTTTAAAATTCAAAACTATTTTTTAAACAAAATATTATTGGAACTCTTTTAAAACTAGATATTAACTAAAATTATTTTTTAAATAAAATATCAACCACTTATTTTTTAATTTTTTAAAAAATTAATGATATTATTGAAATTAAAATATATATATATTAATATGTATATATATATATATATATTAATTAATATACCTATATATATATTTTTTTCAAATAGTCCATAAAATTCTCGCTACCGTTGTAACACGAGCGAGGAGCGGGCTATTTTGGTATTTTCATACAAAACTGGGAAGTGGAAGCCCTAATCTGCACTATATAAATCCTCAATCACACGACGCCCTCATTGCCAAAGTGACTTCGATTTCGATCCAATCTTTTCTTCTCTCTCAACCCTATCTCTGTAATTTCATTTTTTGCTTTTGTTGAAAATTAGGCAGTGAAGGAGATCGCAGTGACGACTCAGTAAAATTCAAGCTCAACAGACCGGATTGCAGAGTCCTTTCCTCTTCCTGA

mRNA sequence

ATGTCCATTTTACTATCAATTTCCCCATCTTTGTCAGCCCCCAACGCCAAGGAGTTATGGTGCAGCCAAACCCCATACCCCGACATCTGCAAGAGCTTCTTCAACGCCAAATTCAACCCCAAAAACCTCGCCGAGTTCAAAAAAGCCGCCTTCAAAATCGCCATGGAAAGGGCTCTCACAGCCGAGTCGCTCACCAAGCGCCACGCCCAGAAATGCCGCACCCCCAAGGAAAAAGGCGCGTGGGCCGACTGCTTGGAGCTTTACCAAGACACCATTCTCCAGCTCAACCGAACCTTCTCCGACAACTATTGCTCCGATTTCGACATCCAAACATGGCTCAGCTCCGCGTTGACTAATCTTGATACATGTCGTGCCGGATTCGTTGACCTTAATATTAAGGATTACGACGTCGTTTTTCCTTTTCTTGGGAGGAGTAATAATGTGACTAAGTTGATTAGGAATAGTTTGGCTATGAATAACTGCTCTGAGACGGAGACGGGTTATCATGGTGGATTTCCTACGTGGGTTAAGGCTGGTGAACGGCGGCTGCTGCAGGCGGCGGTTCCTAAGCCGGACTTGGTTGTGGCGCTTGATGGGTCTGGGAATTATAAGACGGTGCAGGCGGCGGTGGATGCGGCGGCGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATGTTAAGAAAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAGGTCAAGAATTTGATGCTCGTCGGAGATGGGTTGAAGTATACCATTATTACTGGGAGTAGGAGTGTCGGCGGCGGCTCCACCACGTTCAACTCCGCCACCGTCGGCAGTGAAGGAGATCGCAGTGACGACTCAGTAAAATTCAAGCTCAACAGACCGGATTGCAGAGTCCTTTCCTCTTCCTGA

Coding sequence (CDS)

ATGTCCATTTTACTATCAATTTCCCCATCTTTGTCAGCCCCCAACGCCAAGGAGTTATGGTGCAGCCAAACCCCATACCCCGACATCTGCAAGAGCTTCTTCAACGCCAAATTCAACCCCAAAAACCTCGCCGAGTTCAAAAAAGCCGCCTTCAAAATCGCCATGGAAAGGGCTCTCACAGCCGAGTCGCTCACCAAGCGCCACGCCCAGAAATGCCGCACCCCCAAGGAAAAAGGCGCGTGGGCCGACTGCTTGGAGCTTTACCAAGACACCATTCTCCAGCTCAACCGAACCTTCTCCGACAACTATTGCTCCGATTTCGACATCCAAACATGGCTCAGCTCCGCGTTGACTAATCTTGATACATGTCGTGCCGGATTCGTTGACCTTAATATTAAGGATTACGACGTCGTTTTTCCTTTTCTTGGGAGGAGTAATAATGTGACTAAGTTGATTAGGAATAGTTTGGCTATGAATAACTGCTCTGAGACGGAGACGGGTTATCATGGTGGATTTCCTACGTGGGTTAAGGCTGGTGAACGGCGGCTGCTGCAGGCGGCGGTTCCTAAGCCGGACTTGGTTGTGGCGCTTGATGGGTCTGGGAATTATAAGACGGTGCAGGCGGCGGTGGATGCGGCGGCGAAGAGGAAAGGGAGTGGGAGATTTGTGATACATGTTAAGAAAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAGGTCAAGAATTTGATGCTCGTCGGAGATGGGTTGAAGTATACCATTATTACTGGGAGTAGGAGTGTCGGCGGCGGCTCCACCACGTTCAACTCCGCCACCGTCGGCAGTGAAGGAGATCGCAGTGACGACTCAGTAAAATTCAAGCTCAACAGACCGGATTGCAGAGTCCTTTCCTCTTCCTGA

Protein sequence

MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDRSDDSVKFKLNRPDCRVLSSS

Homology

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BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.0e-66
Identity = 139/271 (51.29%), Postives = 181/271 (66.79%), Query Frame = 0


Query: 13  APNAKELWCSQTPYPDICKSFFNAKFNPKNL---AEFKKAAFKIAMERALTAESLTKRHA 72
           +P   + WC +TP P  C+ F   K +  ++    +F K + ++A+ERA TA+S T    
Sbjct: 24  SPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLG 83

Query: 73  QKCRTPKEKGAWADCLELYQDTILQLNRTF-SDNYCSDFDIQTWLSSALTNLDTCRAGFV 132
            KCR  +EK AW DC ELY+ T+L+LN+T  S   C+  D QTWLS+ALTNL+TCRA   
Sbjct: 84  SKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLE 143

Query: 133 DLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGERRLLQAAV 192
           DL + +Y  V P L  SNNVTKLI N+L++N     E  Y  GFPTWVK G+R+LLQ   
Sbjct: 144 DLGVPEY--VLPLL--SNNVTKLISNTLSLNKVPYNEPSYKDGFPTWVKPGDRKLLQ-TT 203

Query: 193 PKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKVKNLMLVG 252
           P+ ++VVA DGSGN KT+Q AV AAA R G  R+VI++K G Y EN+ +  K+KN+M VG
Sbjct: 204 PRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVG 263

Query: 253 DGLKYTIITGSRSVGGGSTTFNSATVGSEGD 280
           DG+  TIITGS+SVGGG+TTF SATV   GD
Sbjct: 264 DGIGKTIITGSKSVGGGATTFKSATVAVVGD 286

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 2.6e-65
Identity = 141/284 (49.65%), Postives = 196/284 (69.01%), Query Frame = 0


Query: 3   ILLSISPSLSAPNAKEL--WCSQTPYPDICKSFF--NAKFNP-KNLAEFKKAAFKIAMER 62
           + + ++ ++S  N K++  WCSQTP P  C+ F   N+   P K+ +EF K + K+ ++R
Sbjct: 14  LCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDR 73

Query: 63  ALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNY-CSDFDIQTWLSSA 122
           A+ A++       KCR  +EK AW DC++LY  T+ ++N T   N  CS  D QTWLS+A
Sbjct: 74  AILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTA 133

Query: 123 LTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETET-GYHGGFPTW 182
           LTNLDTCRAGF++L +   D+V P +  SNNV+ L+ N+LA+N      T     GFP+W
Sbjct: 134 LTNLDTCRAGFLELGVT--DIVLPLM--SNNVSNLLCNTLAINKVPFNYTPPEKDGFPSW 193

Query: 183 VKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 242
           VK G+R+LLQ++ PK + VVA DGSGN+KT++ A+DAA+   GSGRFVI+VK+GVY EN+
Sbjct: 194 VKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENL 253

Query: 243 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 280
            I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATV + GD
Sbjct: 254 EI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGD 288

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.3e-60
Identity = 130/275 (47.27%), Postives = 179/275 (65.09%), Query Frame = 0


Query: 20  WCSQTPYPDICKSFF--NAKFN-PKNLAEFKKAAFKIAMERALTAESLTKRHAQKCRTPK 79
           WC +TPYP  CK +F  ++ F  P  ++EF+    + AM+RA++A       ++ C   K
Sbjct: 41  WCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFK 100

Query: 80  EKGAWADCLELYQDTILQLNRTF------SDNYCSDFDIQTWLSSALTNLDTCRAGFVDL 139
           ++   ADC+ LY DT++QLNRT       +   C+DFD QTWLS+ALTN +TCR G  DL
Sbjct: 101 KQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDL 160

Query: 140 NIKDYDVVFPFLGRSNNVTKLIRNSLAMNNC------SETETGYHGGFPTWVKAGERRLL 199
           N+ D+    P +  +  ++ LI N LA+N        +++ TG   GFPTWV   ERRLL
Sbjct: 161 NVSDFTT--PIVS-NTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLL 220

Query: 200 QAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRK-GSGRFVIHVKKGVYKENVVIGNKVKN 259
           Q    + +LVVA DGSG++KTVQAA+D A +RK  SGRFVI+VK+G+Y+EN+ +     N
Sbjct: 221 QLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDN 280

Query: 260 LMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEG 279
           +MLVGDG++YTIITG RSV GG TT++SAT G EG
Sbjct: 281 IMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.2e-59
Identity = 131/295 (44.41%), Postives = 183/295 (62.03%), Query Frame = 0


Query: 3   ILLSISPSLSAPNAKEL-WCSQTPYPDICKSFF---NAKFNPKNLAEFKKAAFKIAMERA 62
           +LL + P  +  +     WC +TPYPD CK +F   N    P  L+EF+    + AM+RA
Sbjct: 19  VLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVMLVEAAMDRA 78

Query: 63  LTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTF--------SDNYCSDFDIQ 122
           ++A +      + C   K++   ADC++LY DTI+QLNRT         +   C+DFD Q
Sbjct: 79  ISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQ 138

Query: 123 TWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET------ 182
           TWLS+ALTN +TCR G  DLN+ D+  + P +  +  ++ LI N LA+N    T      
Sbjct: 139 TWLSTALTNTETCRRGSSDLNVTDF--ITPIVS-NTKISHLISNCLAVNGALLTAGNKGN 198

Query: 183 ETGYHGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRK-GSGRFV 242
            T    GFPTW+   ++RLL+A   + +LVVA DGSG++ TVQAA+D A +RK  SGRFV
Sbjct: 199 TTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVTSGRFV 258

Query: 243 IHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEG 279
           I+VK+G+Y+EN+ +     ++MLVGDG++ TIITG RSV GG TT+NSAT G EG
Sbjct: 259 IYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 308

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.2e-55
Identity = 124/285 (43.51%), Postives = 173/285 (60.70%), Query Frame = 0


Query: 20  WCSQTPYPDICKSFFNAKFN-------PKNLAEFKKAAFKIAMERALTAESLTKRHAQKC 79
           WC++TP+ + C  +F            P+  +EF +   K+A+++A+   S T +    C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 80  RTPKEKGAWADCLELYQDTILQLNRTF--------SDNYCSDFDIQTWLSSALTNLDTCR 139
              + K AW+DC+ L+Q+T+ QLNRT         SD  C+DFD QTWLS+A TN++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 140 AGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNC-----SETETGYHGG-FPTWVKA 199
           +G  DLN+ D+  V P +  + N++ LI N LA+N         T T  H   FP+WV  
Sbjct: 207 SGSEDLNVSDF--VMPVIS-NKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSR 266

Query: 200 GERRLL---QAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 259
            ERRLL     A   P LVVA D SG+++++QAA++ AA+R+   RFVI+VKKGVY+EN+
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 260 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDR 281
            +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT G  G R
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQR 368

[Close]

BLAST of Cp4.1LG05g07260 vs. NCBI nr
Match: XP_023534348.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552 bits (1423), Expect = 4.18e-194
Identity = 275/279 (98.57%), Postives = 276/279 (98.92%), Query Frame = 0


Query: 1   MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 60
           MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT
Sbjct: 14  MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 73

Query: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120
           AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL
Sbjct: 74  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 133

Query: 121 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180
           DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE
Sbjct: 134 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 193

Query: 181 RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240
           RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK
Sbjct: 194 RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 253

Query: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATV   G+
Sbjct: 254 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 292

[Close]

BLAST of Cp4.1LG05g07260 vs. NCBI nr
Match: KAG6605649.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542 bits (1396), Expect = 3.38e-190
Identity = 268/279 (96.06%), Postives = 273/279 (97.85%), Query Frame = 0


Query: 1   MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 60
           MSILL+ISPS SAPNAK+LWCSQTPYPD+CKSFFNAKFNPKNLAEFKK AFKIAMERALT
Sbjct: 1   MSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFKKTAFKIAMERALT 60

Query: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120
           AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL
Sbjct: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120

Query: 121 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180
           +TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE
Sbjct: 121 ETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180

Query: 181 RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240
           RRLLQAAVPK DLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK
Sbjct: 181 RRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240

Query: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATV   G+
Sbjct: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 279

[Close]

BLAST of Cp4.1LG05g07260 vs. NCBI nr
Match: XP_022957920.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 539 bits (1388), Expect = 8.72e-189
Identity = 267/279 (95.70%), Postives = 272/279 (97.49%), Query Frame = 0


Query: 1   MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 60
           MSILL+ISPS SAPNAK+LWCSQTPYPD+CKSFFNAKFNPKNLAEFKK AFKIAMERALT
Sbjct: 14  MSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFKKTAFKIAMERALT 73

Query: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120
           AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL
Sbjct: 74  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 133

Query: 121 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180
           +TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE
Sbjct: 134 ETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 193

Query: 181 RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240
           RRLLQAAVPK DLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK
Sbjct: 194 RRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 253

Query: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           VKNLMLVGDGLKYTIITGSRSV GGSTTFNSATV   G+
Sbjct: 254 VKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGE 292

[Close]

BLAST of Cp4.1LG05g07260 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 532 bits (1371), Expect = 3.34e-186
Identity = 263/278 (94.60%), Postives = 269/278 (96.76%), Query Frame = 0


Query: 2   SILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALTA 61
           +ILLSISPS SAPNAK+LWCSQTPYPD+CKSFF AKFNPKNLAEFKK AFKIAMERALTA
Sbjct: 15  TILLSISPSFSAPNAKDLWCSQTPYPDVCKSFFKAKFNPKNLAEFKKTAFKIAMERALTA 74

Query: 62  ESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNLD 121
           E+LTKRHAQKCRTPKEKGAWADCLELYQDTIL LNRTFSDNYCSDFDIQTWLSSALTNL+
Sbjct: 75  ETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDFDIQTWLSSALTNLE 134

Query: 122 TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER 181
           TCRAGFVDLNIKDYDVVFPFL RSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER
Sbjct: 135 TCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER 194

Query: 182 RLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV 241
           RLLQAAVPK DLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV
Sbjct: 195 RLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV 254

Query: 242 KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATV   G+
Sbjct: 255 KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 292

[Close]

BLAST of Cp4.1LG05g07260 vs. NCBI nr
Match: KAG7035559.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 400 bits (1027), Expect = 1.55e-138
Identity = 192/199 (96.48%), Postives = 196/199 (98.49%), Query Frame = 0


Query: 1   MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 60
           MSILL+ISPS SAPNAK+LWCSQTPYPD+CKSFFNAKFNPKNLAEFKK AFKIAMERALT
Sbjct: 14  MSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFKKTAFKIAMERALT 73

Query: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120
           AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL
Sbjct: 74  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 133

Query: 121 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180
           +TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE
Sbjct: 134 ETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 193

Query: 181 RRLLQAAVPKPDLVVALDG 199
           RRLLQAAVPK DLVVALDG
Sbjct: 194 RRLLQAAVPKADLVVALDG 212

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 4.22e-189
Identity = 267/279 (95.70%), Postives = 272/279 (97.49%), Query Frame = 0


Query: 1   MSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALT 60
           MSILL+ISPS SAPNAK+LWCSQTPYPD+CKSFFNAKFNPKNLAEFKK AFKIAMERALT
Sbjct: 14  MSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFKKTAFKIAMERALT 73

Query: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120
           AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL
Sbjct: 74  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 133

Query: 121 DTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180
           +TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE
Sbjct: 134 ETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 193

Query: 181 RRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240
           RRLLQAAVPK DLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK
Sbjct: 194 RRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 253

Query: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           VKNLMLVGDGLKYTIITGSRSV GGSTTFNSATV   G+
Sbjct: 254 VKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGE 292

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 1.62e-186
Identity = 263/278 (94.60%), Postives = 269/278 (96.76%), Query Frame = 0


Query: 2   SILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALTA 61
           +ILLSISPS SAPNAK+LWCSQTPYPD+CKSFF AKFNPKNLAEFKK AFKIAMERALTA
Sbjct: 15  TILLSISPSFSAPNAKDLWCSQTPYPDVCKSFFKAKFNPKNLAEFKKTAFKIAMERALTA 74

Query: 62  ESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNLD 121
           E+LTKRHAQKCRTPKEKGAWADCLELYQDTIL LNRTFSDNYCSDFDIQTWLSSALTNL+
Sbjct: 75  ETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDFDIQTWLSSALTNLE 134

Query: 122 TCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER 181
           TCRAGFVDLNIKDYDVVFPFL RSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER
Sbjct: 135 TCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGER 194

Query: 182 RLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV 241
           RLLQAAVPK DLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV
Sbjct: 195 RLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKV 254

Query: 242 KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 279
           KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATV   G+
Sbjct: 255 KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 292

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 396 bits (1017), Expect = 8.55e-133
Identity = 213/286 (74.48%), Postives = 232/286 (81.12%), Query Frame = 0


Query: 1   MSILLSIS-PSLSAPNAK--ELWCSQTPYPDICKSFFN-AKFNPKNLAEFKKAAFKIAME 60
           M IL S S P  +  N K  ELWCS+TPYPD+CK FFN  +F+P+NL + KKAA KIAME
Sbjct: 14  MFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRNLLDIKKAALKIAME 73

Query: 61  RALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSA 120
           RA+  E+LTK   QKCR  KE+ AWADCLELYQ TIL LN+TFSD  CS+FDIQTWLSSA
Sbjct: 74  RAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKNCSNFDIQTWLSSA 133

Query: 121 LTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHG--GFPT 180
           LTNL TCRAGFVDL IKDY VVFPFL  +NN+TKLI NSLAMNNCSE++ G     GFP 
Sbjct: 134 LTNLHTCRAGFVDLGIKDYGVVFPFL-ENNNITKLISNSLAMNNCSESDEGNTSDEGFPK 193

Query: 181 WVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKEN 240
           W+  G+RRLLQAA PK DLVVA DGSGNYKTVQAAVDAA KRKGSGRFVI VKKGVYKEN
Sbjct: 194 WLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRVKKGVYKEN 253

Query: 241 VVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDR 280
           VVI  KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATV   G+R
Sbjct: 254 VVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGER 296

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 4.72e-132
Identity = 209/284 (73.59%), Postives = 232/284 (81.69%), Query Frame = 0


Query: 3   ILLSISPSLSAPNAK---ELWCSQTPYPDICKSFFN-AKFNPKNLAEFKKAAFKIAMERA 62
           IL S S   S    K   +LWCS+TPYPD+CK+FFN  +F+PKNL + +K A KIAMERA
Sbjct: 16  ILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKNLLDIQKTALKIAMERA 75

Query: 63  LTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALT 122
           +  E+LTK   QKCR  KE+ AWADCLELYQ TIL LN+TFSD +CS+FDIQTWLSSALT
Sbjct: 76  IRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSD-HCSNFDIQTWLSSALT 135

Query: 123 NLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHG--GFPTWV 182
           NLDTCRAGFVDL IKDY VVFPFL  +NN+TKLI NSLAMNNCSE++ G     GFP WV
Sbjct: 136 NLDTCRAGFVDLGIKDYGVVFPFLA-NNNITKLISNSLAMNNCSESDEGNTSDEGFPKWV 195

Query: 183 KAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVV 242
           + G+RRLLQAA PK DLVVA DGSGNYKTVQAAVDAA KRKGSGRFVI +KKGVYKENVV
Sbjct: 196 RGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYKENVV 255

Query: 243 IGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDR 280
           I  KVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATV   G+R
Sbjct: 256 I--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGER 295

[Close]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL
Match: A0A6J1DZE7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111025994 PE=3 SV=1)

HSP 1 Score: 345 bits (886), Expect = 4.09e-113
Identity = 186/277 (67.15%), Postives = 218/277 (78.70%), Query Frame = 0


Query: 7   ISPSLSAPNAKELWCSQTPYPDICKSFFNAK-FNPKNLAEFKKAAFKIAMERALTAESLT 66
           +SPSLS  NA + WCS+TPYPD+C   FNAK FNP  L+EFK  A KIA++ AL  E+LT
Sbjct: 20  VSPSLST-NAVDTWCSRTPYPDVCNHIFNAKHFNPTKLSEFKTMARKIALDTALATETLT 79

Query: 67  KRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNLDTCRA 126
           K+      T KE  A ADCL LY+ TILQLN+TFS   CSDFDIQTWLSSALTNLDTCRA
Sbjct: 80  KQLGLNSPTEKESAALADCLHLYKITILQLNKTFSHK-CSDFDIQTWLSSALTNLDTCRA 139

Query: 127 GFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET--ETGYHGGFPTWVKAGERRL 186
           GF DL+     V FP L  +NNVTKLI NSLAMNN S++  ETGY  GFPTWV+A +RRL
Sbjct: 140 GFADLH-----VAFPLLLNNNNVTKLISNSLAMNNYSKSNSETGYRRGFPTWVRARDRRL 199

Query: 187 LQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNKVKN 246
           LQA  PK ++VVA DGSGNY+T+ AA+ AA +R GSGRFVI+VKKGVY+ENVVIGN++KN
Sbjct: 200 LQAESPKANVVVAQDGSGNYRTIAAAL-AAGRRSGSGRFVIYVKKGVYEENVVIGNEMKN 259

Query: 247 LMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDR 280
           +ML+GDGL+YTI+TGSRSVGGGSTTFNSATV   G+R
Sbjct: 260 IMLLGDGLRYTIVTGSRSVGGGSTTFNSATVFVSGER 288

[Close]

BLAST of Cp4.1LG05g07260 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-66
Identity = 141/284 (49.65%), Postives = 196/284 (69.01%), Query Frame = 0


Query: 3   ILLSISPSLSAPNAKEL--WCSQTPYPDICKSFF--NAKFNP-KNLAEFKKAAFKIAMER 62
           + + ++ ++S  N K++  WCSQTP P  C+ F   N+   P K+ +EF K + K+ ++R
Sbjct: 14  LCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDR 73

Query: 63  ALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNY-CSDFDIQTWLSSA 122
           A+ A++       KCR  +EK AW DC++LY  T+ ++N T   N  CS  D QTWLS+A
Sbjct: 74  AILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTA 133

Query: 123 LTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETET-GYHGGFPTW 182
           LTNLDTCRAGF++L +   D+V P +  SNNV+ L+ N+LA+N      T     GFP+W
Sbjct: 134 LTNLDTCRAGFLELGVT--DIVLPLM--SNNVSNLLCNTLAINKVPFNYTPPEKDGFPSW 193

Query: 183 VKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 242
           VK G+R+LLQ++ PK + VVA DGSGN+KT++ A+DAA+   GSGRFVI+VK+GVY EN+
Sbjct: 194 VKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENL 253

Query: 243 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGD 280
            I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATV + GD
Sbjct: 254 EI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGD 288

[Close]

BLAST of Cp4.1LG05g07260 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 233.4 bits (594), Expect = 2.4e-61
Identity = 130/275 (47.27%), Postives = 179/275 (65.09%), Query Frame = 0


Query: 20  WCSQTPYPDICKSFF--NAKFN-PKNLAEFKKAAFKIAMERALTAESLTKRHAQKCRTPK 79
           WC +TPYP  CK +F  ++ F  P  ++EF+    + AM+RA++A       ++ C   K
Sbjct: 41  WCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFK 100

Query: 80  EKGAWADCLELYQDTILQLNRTF------SDNYCSDFDIQTWLSSALTNLDTCRAGFVDL 139
           ++   ADC+ LY DT++QLNRT       +   C+DFD QTWLS+ALTN +TCR G  DL
Sbjct: 101 KQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDL 160

Query: 140 NIKDYDVVFPFLGRSNNVTKLIRNSLAMNNC------SETETGYHGGFPTWVKAGERRLL 199
           N+ D+    P +  +  ++ LI N LA+N        +++ TG   GFPTWV   ERRLL
Sbjct: 161 NVSDFTT--PIVS-NTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLL 220

Query: 200 QAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRK-GSGRFVIHVKKGVYKENVVIGNKVKN 259
           Q    + +LVVA DGSG++KTVQAA+D A +RK  SGRFVI+VK+G+Y+EN+ +     N
Sbjct: 221 QLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDN 280

Query: 260 LMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEG 279
           +MLVGDG++YTIITG RSV GG TT++SAT G EG
Sbjct: 281 IMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

[Close]

BLAST of Cp4.1LG05g07260 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 228.8 bits (582), Expect = 5.8e-60
Identity = 131/295 (44.41%), Postives = 183/295 (62.03%), Query Frame = 0


Query: 3   ILLSISPSLSAPNAKEL-WCSQTPYPDICKSFF---NAKFNPKNLAEFKKAAFKIAMERA 62
           +LL + P  +  +     WC +TPYPD CK +F   N    P  L+EF+    + AM+RA
Sbjct: 19  VLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVMLVEAAMDRA 78

Query: 63  LTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTF--------SDNYCSDFDIQ 122
           ++A +      + C   K++   ADC++LY DTI+QLNRT         +   C+DFD Q
Sbjct: 79  ISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQ 138

Query: 123 TWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET------ 182
           TWLS+ALTN +TCR G  DLN+ D+  + P +  +  ++ LI N LA+N    T      
Sbjct: 139 TWLSTALTNTETCRRGSSDLNVTDF--ITPIVS-NTKISHLISNCLAVNGALLTAGNKGN 198

Query: 183 ETGYHGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRK-GSGRFV 242
            T    GFPTW+   ++RLL+A   + +LVVA DGSG++ TVQAA+D A +RK  SGRFV
Sbjct: 199 TTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVTSGRFV 258

Query: 243 IHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEG 279
           I+VK+G+Y+EN+ +     ++MLVGDG++ TIITG RSV GG TT+NSAT G EG
Sbjct: 259 IYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 308

[Close]

BLAST of Cp4.1LG05g07260 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-56
Identity = 124/285 (43.51%), Postives = 173/285 (60.70%), Query Frame = 0


Query: 20  WCSQTPYPDICKSFFNAKFN-------PKNLAEFKKAAFKIAMERALTAESLTKRHAQKC 79
           WC++TP+ + C  +F            P+  +EF +   K+A+++A+   S T +    C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 80  RTPKEKGAWADCLELYQDTILQLNRTF--------SDNYCSDFDIQTWLSSALTNLDTCR 139
              + K AW+DC+ L+Q+T+ QLNRT         SD  C+DFD QTWLS+A TN++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 140 AGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNC-----SETETGYHGG-FPTWVKA 199
           +G  DLN+ D+  V P +  + N++ LI N LA+N         T T  H   FP+WV  
Sbjct: 207 SGSEDLNVSDF--VMPVIS-NKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSR 266

Query: 200 GERRLL---QAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 259
            ERRLL     A   P LVVA D SG+++++QAA++ AA+R+   RFVI+VKKGVY+EN+
Sbjct: 267 HERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENI 326

Query: 260 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVGSEGDR 281
            +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT G  G R
Sbjct: 327 DVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQR 368

[Close]

BLAST of Cp4.1LG05g07260 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 154.8 bits (390), Expect = 1.1e-37
Identity = 99/262 (37.79%), Postives = 150/262 (57.25%), Query Frame = 0


Query: 21  CSQTPYPDICKSFFNAKFNPKNLAEFKKAAFKIAMERALTAESLTKRHAQKCRTPKEKGA 80
           C +TP+P  CK+        ++  +F + + +  ++ A+ A+S T     +  +   K A
Sbjct: 8   CDETPHPGECKTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS---KQA 67

Query: 81  WADCLELYQDTILQLNRTF--SDNYCSDFDIQTWLSSALTNLDTCRAGFVDLNIKDYDVV 140
           W +C++LY+ TI +LN +     N CS  D+Q WLS+ALTNLDTC+    +L +  + + 
Sbjct: 68  WEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVSSHSL- 127

Query: 141 FPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGERRLLQAAVPKPDLVVALD 200
                   ++T  + N+LA+N   E      G     +K        +   K D+VVA D
Sbjct: 128 -------ESITIDVINTLAINKRMEQNGKEFGISKITMKT------LSIGEKVDVVVAQD 187

Query: 201 GSGNYKTVQAAVDAAAKR-KGSGRFVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIIT 260
           GSG+YKT+Q AV+ A +R KGS R+VIHVK+GVY+E V +G K  N+M+ GDG+  TIIT
Sbjct: 188 GSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIGKTIIT 247

Query: 261 GSRSVGGGSTTFNSATVGSEGD 280
           G +S G G +T+ SAT  +EGD
Sbjct: 248 GDKSKGRGFSTYKSATFVAEGD 252

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g07260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	9.0e-66	51.29	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	2.6e-65	49.65	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	3.3e-60	47.27	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	8.2e-59	44.41	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	3.2e-55	43.51	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cp4.1LG05g07260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534348.1	4.18e-194	98.57	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605649.1	3.38e-190	96.06	Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022957920.1	8.72e-189	95.70	pectinesterase-like [Cucurbita moschata]	[more]
XP_022996028.1	3.34e-186	94.60	pectinesterase-like [Cucurbita maxima]	[more]
KAG7035559.1	1.55e-138	96.48	Pectinesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cp4.1LG05g07260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1Z9	4.22e-189	95.70	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1	[more]
A0A6J1K9P3	1.62e-186	94.60	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1	[more]
A0A0A0KPG9	8.55e-133	74.48	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1	[more]
A0A1S3ASJ4	4.72e-132	73.59	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1	[more]
A0A6J1DZE7	4.09e-113	67.15	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111025994 PE=3 SV=1	[more]

BLAST of Cp4.1LG05g07260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	1.9e-66	49.65	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	2.4e-61	47.27	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	5.8e-60	44.41	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	2.3e-56	43.51	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	1.1e-37	37.79	pectin methylesterase 38	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 9..158 e-value: 1.3E-29 score: 114.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 18..160 e-value: 1.8E-21 score: 74.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 20..158 e-value: 3.2E-27 score: 95.7
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 192..280 e-value: 2.2E-26 score: 92.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 187..289 e-value: 1.8E-26 score: 95.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 18..161 e-value: 1.6E-30 score: 108.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 20..161
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 4..279
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 4..279
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 19..160 e-value: 7.85853E-38 score: 129.48
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 218..237
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 187..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g07260.1	Cp4.1LG05g07260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity