Cp4.1LG04g10160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g10160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin-protein ligase ATL42-like
LocationCp4.1LG04: 9621667 .. 9622860 (+)
RNA-Seq ExpressionCp4.1LG04g10160
SyntenyCp4.1LG04g10160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCCGCTTAAATTTGGTCACTTTTTGCTTCTTTTCTGTGTTGTTCTATGTCCAAGCTCAACCTTCTTCTCAAGATGCTGAAAACTATTCATTTCAGCCAAATCTTGGGTTTGTGATAGGAATTCTAGGCGTTATGTTTGTGTTGACATTTGTTCTTCTTGTTTATGCAAAGTACTGCCATAACAGATCTTCTGTTCATGTGAATAATGGTGTAACCCATGGCGGACAAATCAGATCAAGCTCAAGCACACCCATTTTGGGAATCGACAAGACAGTGATCGAGTCCCTTCCATTTTTTAGATTCTCCACTCTTAAAGGCTCCAAACGAGGGCTTGAATGTGCTATCTGTCTTTCCAAATTTGAAGATATCGAAATTCTCCGGCTGTTGCCTAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTTGAAAGACATGCCACCTGCCCTCTTTGCAGGCAGGGAGTTAGCTCAGAGGATTTGCAGCTCTTTACTAATTCAAGCAGTATGAGGTTCTTATGGAGCAATTTATCAGAGCTGAAGCAAGATTCAAACATAGAGCTGTTTGTACAGAGAGAAGAAGAAGAAGAAGAACATCAACAACAACAACAACAGATCCATGGCTCTTTTAGATTCAGCATTGGGAGAAGCTTCAGAAAGATGTTGAAGAGTGACAAAGAAGGGGAAATGTTGATTCAAAAGGATTATGATGGAGATAAGAACATGAAGAACTTACACAGACATAATCACAAGATCATCATATCTGATTTTGTCTTCATGAATCGATGGAGCAATGTTAGTTCATCAGACATGATGTTCTTGAATAAAGAGATGATTGATTCCATTTCAAGCGGTAGATTTACTTCCTTGGAAACATATTTAGAGGAGTCAACGCTATCAAGATCAGTAGAAAATGAAGAACCCTTGAAGGGATTTCCATCTACATCAGATTGTTCAGCAAGAATTACAAGCACAGAAGCGAGAAGATCCGTTTCAGAAATCACAGGCGTGACAAGATTTGGTGGTGATGTTCATATGAATTTCAACAGAAAAATTGAAATGGGGGAATCTTCTGAGCTGGAAAACAATGTGAAGAGACAAACTTGGTACCCAATTGCTAGAAAGACAGTCCAATGGTTTGCCAATAGAGAAAAAACAAGACAACACAGAGTAGAAACTGTATAA

mRNA sequence

ATGATCCGCTTAAATTTGGTCACTTTTTGCTTCTTTTCTGTGTTGTTCTATGTCCAAGCTCAACCTTCTTCTCAAGATGCTGAAAACTATTCATTTCAGCCAAATCTTGGGTTTGTGATAGGAATTCTAGGCGTTATGTTTGTGTTGACATTTGTTCTTCTTGTTTATGCAAAGTACTGCCATAACAGATCTTCTGTTCATGTGAATAATGGTGTAACCCATGGCGGACAAATCAGATCAAGCTCAAGCACACCCATTTTGGGAATCGACAAGACAGTGATCGAGTCCCTTCCATTTTTTAGATTCTCCACTCTTAAAGGCTCCAAACGAGGGCTTGAATGTGCTATCTGTCTTTCCAAATTTGAAGATATCGAAATTCTCCGGCTGTTGCCTAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTTGAAAGACATGCCACCTGCCCTCTTTGCAGGCAGGGAGTTAGCTCAGAGGATTTGCAGCTCTTTACTAATTCAAGCAGTATGAGGTTCTTATGGAGCAATTTATCAGAGCTGAAGCAAGATTCAAACATAGAGCTGTTTGTACAGAGAGAAGAAGAAGAAGAAGAACATCAACAACAACAACAACAGATCCATGGCTCTTTTAGATTCAGCATTGGGAGAAGCTTCAGAAAGATGTTGAAGAGTGACAAAGAAGGGGAAATGTTGATTCAAAAGGATTATGATGGAGATAAGAACATGAAGAACTTACACAGACATAATCACAAGATCATCATATCTGATTTTGTCTTCATGAATCGATGGAGCAATGTTAGTTCATCAGACATGATGTTCTTGAATAAAGAGATGATTGATTCCATTTCAAGCGGTAGATTTACTTCCTTGGAAACATATTTAGAGGAGTCAACGCTATCAAGATCAGTAGAAAATGAAGAACCCTTGAAGGGATTTCCATCTACATCAGATTGTTCAGCAAGAATTACAAGCACAGAAGCGAGAAGATCCGTTTCAGAAATCACAGGCGTGACAAGATTTGGTGGTGATGTTCATATGAATTTCAACAGAAAAATTGAAATGGGGGAATCTTCTGAGCTGGAAAACAATGTGAAGAGACAAACTTGGTACCCAATTGCTAGAAAGACAGTCCAATGGTTTGCCAATAGAGAAAAAACAAGACAACACAGAGTAGAAACTGTATAA

Coding sequence (CDS)

ATGATCCGCTTAAATTTGGTCACTTTTTGCTTCTTTTCTGTGTTGTTCTATGTCCAAGCTCAACCTTCTTCTCAAGATGCTGAAAACTATTCATTTCAGCCAAATCTTGGGTTTGTGATAGGAATTCTAGGCGTTATGTTTGTGTTGACATTTGTTCTTCTTGTTTATGCAAAGTACTGCCATAACAGATCTTCTGTTCATGTGAATAATGGTGTAACCCATGGCGGACAAATCAGATCAAGCTCAAGCACACCCATTTTGGGAATCGACAAGACAGTGATCGAGTCCCTTCCATTTTTTAGATTCTCCACTCTTAAAGGCTCCAAACGAGGGCTTGAATGTGCTATCTGTCTTTCCAAATTTGAAGATATCGAAATTCTCCGGCTGTTGCCTAAGTGCAAGCACGCGTTTCATATCAACTGCATCGACCATTGGCTTGAAAGACATGCCACCTGCCCTCTTTGCAGGCAGGGAGTTAGCTCAGAGGATTTGCAGCTCTTTACTAATTCAAGCAGTATGAGGTTCTTATGGAGCAATTTATCAGAGCTGAAGCAAGATTCAAACATAGAGCTGTTTGTACAGAGAGAAGAAGAAGAAGAAGAACATCAACAACAACAACAACAGATCCATGGCTCTTTTAGATTCAGCATTGGGAGAAGCTTCAGAAAGATGTTGAAGAGTGACAAAGAAGGGGAAATGTTGATTCAAAAGGATTATGATGGAGATAAGAACATGAAGAACTTACACAGACATAATCACAAGATCATCATATCTGATTTTGTCTTCATGAATCGATGGAGCAATGTTAGTTCATCAGACATGATGTTCTTGAATAAAGAGATGATTGATTCCATTTCAAGCGGTAGATTTACTTCCTTGGAAACATATTTAGAGGAGTCAACGCTATCAAGATCAGTAGAAAATGAAGAACCCTTGAAGGGATTTCCATCTACATCAGATTGTTCAGCAAGAATTACAAGCACAGAAGCGAGAAGATCCGTTTCAGAAATCACAGGCGTGACAAGATTTGGTGGTGATGTTCATATGAATTTCAACAGAAAAATTGAAATGGGGGAATCTTCTGAGCTGGAAAACAATGTGAAGAGACAAACTTGGTACCCAATTGCTAGAAAGACAGTCCAATGGTTTGCCAATAGAGAAAAAACAAGACAACACAGAGTAGAAACTGTATAA

Protein sequence

MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEESTLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGESSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Homology
BLAST of Cp4.1LG04g10160 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 5.7e-84
Identity = 187/396 (47.22%), Postives = 248/396 (62.63%), Query Frame = 0

Query: 31  SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRSSVHVNNGVTHGGQIR--------SSS 90
           +F+P+L  V G+L +MF LTFVLLVYAK CH        +   H  ++R        ++S
Sbjct: 32  NFEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTAS 91

Query: 91  STPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRLLPKCKHAFHINCI 150
           S    G+DKT IESLP FRFS LKGSK+GL+C++CLSKFE +EILRLLPKC+HAFHI CI
Sbjct: 92  SDRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCI 151

Query: 151 DHWLERHATCPLCRQGVS-SEDLQLFTNSSSMRFLWSNLSELKQDSNIELFVQREEEEEE 210
           D WLE+HATCPLCR  VS  ED  + TN +S RFL  N SE+++DS++EL+++REEEEE 
Sbjct: 152 DQWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEE- 211

Query: 211 HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGDKNMKNLHRHNHKIIISDFV 270
            +  ++++ GS RFSIG SFRK+LK   + + L+ +  +     K +H+ NH+I++SD V
Sbjct: 212 -RIHREELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDVV 271

Query: 271 FMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLE--------------TYLEESTLSRSVE 330
           F NRWSNVSSSD+MFLN EM++SISS RF+SL+                 EE    R +E
Sbjct: 272 FKNRWSNVSSSDLMFLNSEMVNSISSERFSSLDHVKRGDEEDQIGILRIKEEMEAKRMLE 331

Query: 331 NE--EPLKGFPS----TSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGESS 390
           N+       F S    +   S  +     RRSVS+IT V R    +H + +       +S
Sbjct: 332 NKLTSMTTMFSSENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGS-AAETAS 391

Query: 391 ELEN------NVKRQTWYPIARKTVQWFANREKTRQ 392
            L+N        +R+ W PIARKT QWFANREK  Q
Sbjct: 392 ALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Cp4.1LG04g10160 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.6e-70
Identity = 171/403 (42.43%), Postives = 243/403 (60.30%), Query Frame = 0

Query: 7   VTFCFFSVLF--YVQAQ---PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCH 66
           ++  FF ++F  YV AQ   P +  A +  F+P+L  + G+  ++F LTFVLLVYAK  H
Sbjct: 7   ISILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH 66

Query: 67  NRSSVHVNNGVTHGGQIRS--------SSSTPILGIDKTVIESLPFFRFSTLKGSKRGLE 126
           N      +   + G +IR         + S+   G+DK  IESLPFFRFS LKG K+GLE
Sbjct: 67  NDLRSETD---SDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLE 126

Query: 127 CAICLSKFEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSE-DLQLFTNSSS 186
           C++CLSKFED+EILRLLPKC+HAFHI CID WLE+HATCPLCR  V+ E DL +  NSS+
Sbjct: 127 CSVCLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSST 186

Query: 187 MRFLWSNLSELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGE 246
              + +     ++DS +E++++REE   +         GS RFS   SFRK+LK      
Sbjct: 187 SLRILNQSETREEDSRLEIYIEREEGTND---------GSSRFS---SFRKILKK----S 246

Query: 247 MLIQKDYDGDKNM---KNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGR 306
           +L+++  +G++N+   K +H+ NH+I++SD VF NRWSN++SSD+ FL  EM++S+SS R
Sbjct: 247 LLLER--EGNENIDEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDR 306

Query: 307 FTSLETYLEESTLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTR---FGGD 366
           F+S++  +    L R  E+ E  +      D S        RR+VSEIT V+R    GG 
Sbjct: 307 FSSVDR-VHRGNL-RDKEDMEMKRMLIKHKDSS--------RRTVSEITTVSREKAVGGS 366

Query: 367 VHMNFNRKIEMGESSELENNVKRQTWYPIARKTVQWFANREKT 390
              +     +    +      +R+ W PIAR+T QWF NREK+
Sbjct: 367 YRGSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNREKS 378

BLAST of Cp4.1LG04g10160 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 114/317 (35.96%), Postives = 155/317 (48.90%), Query Frame = 0

Query: 22  PSSQDAENY--SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRS-SVHVNN-------- 81
           PS     N+  S  P +  VI +L   F LTF+LL+Y K+C  R+ SV+VN+        
Sbjct: 42  PSPPPRHNFTSSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITR 101

Query: 82  ---GVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEIL 141
              G  +GG +         GID++VIESLP FRF  L G K GLECA+CL++FE  E+L
Sbjct: 102 YGGGYYNGGGVVGGRKNS--GIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVL 161

Query: 142 RLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSELKQDS 201
           RLLPKCKHAFH+ C+D WL+ H+TCPLCR  V  ED+ L  + +S    W  L   K +S
Sbjct: 162 RLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDES 221

Query: 202 N--------------IELFVQREEEEEEHQQQQQQIHGSFRF-SIGRSFRKMLKSD---- 261
           N              +     R     E   +  +I  S  + S   SFR+ L S     
Sbjct: 222 NSVNNNPPGLTRFIPVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVN 281

Query: 262 --------------------KEGEMLI--QKDYDGDKNMKNLHRHNHKIIISDFVFMN-R 283
                               K+G +LI  ++ ++G        R  H+IIIS     + R
Sbjct: 282 DAGEEKSESVAVNCLDRLQRKDGLLLIPNRESFEG--------RFEHRIIISGGNRDDQR 341

BLAST of Cp4.1LG04g10160 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 7.3e-23
Identity = 60/148 (40.54%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 22  PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC----HNRSSVHVNNGVTHGGQ 81
           PS  D +     P+   V  +L  +F LT +L VY ++C     + S+ +  N    G  
Sbjct: 33  PSPPDLQT-GHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSS 92

Query: 82  IRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRG---LECAICLSKFEDIEILRLLPKCK 141
            R        G+D  V+ES P F +S++K SK G   LECAICL++ ED E +RLLP C 
Sbjct: 93  RRG-------GLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICN 152

Query: 142 HAFHINCIDHWLERHATCPLCRQGVSSE 163
           H FHI+CID WL  HATCP+CR  ++++
Sbjct: 153 HLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Cp4.1LG04g10160 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.2e-22
Identity = 57/151 (37.75%), Postives = 90/151 (59.60%), Query Frame = 0

Query: 16  FYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC--HNRSSVHVNNGVT 75
           F++    SS  +   SF P L  +IGIL    +L     + +KYC  H+++S      + 
Sbjct: 41  FFLDDDSSSSSS---SFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLN 100

Query: 76  HGGQIRSSSSTPIL----GIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRL 135
           H G+   SS+  I     G+++++I+S+  +++ +  G   G +C++CLS+FE+ E LRL
Sbjct: 101 HNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRL 160

Query: 136 LPKCKHAFHINCIDHWLERHATCPLCRQGVS 161
           LPKC HAFH+ CID WL+ H+ CPLCR  V+
Sbjct: 161 LPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

BLAST of Cp4.1LG04g10160 vs. NCBI nr
Match: XP_023531707.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 781 bits (2018), Expect = 6.66e-285
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYD 240
           SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYD
Sbjct: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYD 240

Query: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEES 300
           GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEES
Sbjct: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEES 300

Query: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGES 360
           TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGES
Sbjct: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGES 360

Query: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397

BLAST of Cp4.1LG04g10160 vs. NCBI nr
Match: KAG7021875.1 (E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 766 bits (1978), Expect = 8.64e-279
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEE-HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEE H QQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHM FNR+IEMGE
Sbjct: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 398

BLAST of Cp4.1LG04g10160 vs. NCBI nr
Match: XP_022929946.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita moschata])

HSP 1 Score: 765 bits (1975), Expect = 1.08e-277
Identity = 390/398 (97.99%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEEEE-HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEE H QQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGE 360
           STLSRSVENEEPL+GFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHM FNR+IEMGE
Sbjct: 340 STLSRSVENEEPLRGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 399

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 400 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 437

BLAST of Cp4.1LG04g10160 vs. NCBI nr
Match: XP_023006562.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita maxima])

HSP 1 Score: 759 bits (1961), Expect = 3.13e-276
Identity = 388/397 (97.73%), Postives = 391/397 (98.49%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYD 240
           SELKQDSNIELFVQREEEEEE + QQQQIHGSFRFSIGRSFRKMLK DKEGEMLIQKDYD
Sbjct: 181 SELKQDSNIELFVQREEEEEE-EHQQQQIHGSFRFSIGRSFRKMLKGDKEGEMLIQKDYD 240

Query: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEES 300
           GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFT LETYLEES
Sbjct: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTFLETYLEES 300

Query: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGES 360
           TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGV+RFGGDVHM FNRKIEMGES
Sbjct: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVSRFGGDVHMKFNRKIEMGES 360

Query: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 396

BLAST of Cp4.1LG04g10160 vs. NCBI nr
Match: KAG6587983.1 (E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 766 bits (1978), Expect = 7.44e-276
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 183 MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 242

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 243 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 302

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 303 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 362

Query: 181 SELKQDSNIELFVQREEEEEE-HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEE H QQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 363 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 422

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 423 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 482

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHM FNR+IEMGE
Sbjct: 483 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 542

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 543 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 580

BLAST of Cp4.1LG04g10160 vs. ExPASy TrEMBL
Match: A0A6J1EPJ8 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita moschata OX=3662 GN=LOC111436403 PE=4 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 5.21e-278
Identity = 390/398 (97.99%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEEEE-HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEE H QQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGE 360
           STLSRSVENEEPL+GFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHM FNR+IEMGE
Sbjct: 340 STLSRSVENEEPLRGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 399

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 400 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 437

BLAST of Cp4.1LG04g10160 vs. ExPASy TrEMBL
Match: A0A6J1KW64 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita maxima OX=3661 GN=LOC111499247 PE=4 SV=1)

HSP 1 Score: 759 bits (1961), Expect = 1.51e-276
Identity = 388/397 (97.73%), Postives = 391/397 (98.49%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYD 240
           SELKQDSNIELFVQREEEEEE + QQQQIHGSFRFSIGRSFRKMLK DKEGEMLIQKDYD
Sbjct: 181 SELKQDSNIELFVQREEEEEE-EHQQQQIHGSFRFSIGRSFRKMLKGDKEGEMLIQKDYD 240

Query: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEES 300
           GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFT LETYLEES
Sbjct: 241 GDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTFLETYLEES 300

Query: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGES 360
           TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGV+RFGGDVHM FNRKIEMGES
Sbjct: 301 TLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVSRFGGDVHMKFNRKIEMGES 360

Query: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397
           SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 396

BLAST of Cp4.1LG04g10160 vs. ExPASy TrEMBL
Match: A0A5D3DZ48 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00770 PE=4 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 2.86e-184
Identity = 300/443 (67.72%), Postives = 346/443 (78.10%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MI+L+L     FSV F V+AQ  SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           H R+S+ V+ GV H  QIRSSS     GIDKTVIESLPFFRFSTLKG+K GLECA+CLSK
Sbjct: 61  HRRASISVD-GVNHPRQIRSSSR--FSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L +NSSSMRFL SNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQK--- 240
           SELKQDSNIELFVQREEEE   QQQQQ +HGS RFSI RSFRK+LK+DKE EMLI +   
Sbjct: 181 SELKQDSNIELFVQREEEE---QQQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQASC 240

Query: 241 DYDGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYL 300
           DY+ +K MKNLHRHNHKII+SDFVFMNRWSN+SSSD+MFLNKEMID+ISS RFTSL+T +
Sbjct: 241 DYEDEK-MKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTDI 300

Query: 301 EESTLSRSVENEEPLK-------------------------GFPSTSDCSA------RIT 360
           E+STL RS++ +E LK                         G+PSTS  S       RIT
Sbjct: 301 EQSTLPRSMQIDEILKIKEEMEIKRSFESKLNTKIQSNSILGYPSTSQSSTNPSQTTRIT 360

Query: 361 STEARRSVSEITGVTRFG--GDVHMNFNRKIEMGESSELENNVK----RQTWYPIARKTV 397
           S +ARRSVSEITG++RFG   D++MNFNRK+E GESS+LE++VK    R+ WYPIA++TV
Sbjct: 361 SPDARRSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRTV 420

BLAST of Cp4.1LG04g10160 vs. ExPASy TrEMBL
Match: A0A1S3CHK3 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500995 PE=4 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 2.86e-184
Identity = 300/443 (67.72%), Postives = 346/443 (78.10%), Query Frame = 0

Query: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MI+L+L     FSV F V+AQ  SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120
           H R+S+ V+ GV H  QIRSSS     GIDKTVIESLPFFRFSTLKG+K GLECA+CLSK
Sbjct: 61  HRRASISVD-GVNHPRQIRSSSR--FSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L +NSSSMRFL SNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQK--- 240
           SELKQDSNIELFVQREEEE   QQQQQ +HGS RFSI RSFRK+LK+DKE EMLI +   
Sbjct: 181 SELKQDSNIELFVQREEEE---QQQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQASC 240

Query: 241 DYDGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYL 300
           DY+ +K MKNLHRHNHKII+SDFVFMNRWSN+SSSD+MFLNKEMID+ISS RFTSL+T +
Sbjct: 241 DYEDEK-MKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTDI 300

Query: 301 EESTLSRSVENEEPLK-------------------------GFPSTSDCSA------RIT 360
           E+STL RS++ +E LK                         G+PSTS  S       RIT
Sbjct: 301 EQSTLPRSMQIDEILKIKEEMEIKRSFESKLNTKIQSNSILGYPSTSQSSTNPSQTTRIT 360

Query: 361 STEARRSVSEITGVTRFG--GDVHMNFNRKIEMGESSELENNVK----RQTWYPIARKTV 397
           S +ARRSVSEITG++RFG   D++MNFNRK+E GESS+LE++VK    R+ WYPIA++TV
Sbjct: 361 SPDARRSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRTV 420

BLAST of Cp4.1LG04g10160 vs. ExPASy TrEMBL
Match: A0A6J1CUL6 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014427 PE=4 SV=1)

HSP 1 Score: 525 bits (1351), Expect = 4.37e-183
Identity = 291/435 (66.90%), Postives = 335/435 (77.01%), Query Frame = 0

Query: 3   RLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCHN 62
           RL+LV FC FSV F+V+AQ   QD+EN +FQP+LGFVIGILGVMF+LTFVLL+YAK+CH 
Sbjct: 3   RLSLVAFCLFSVFFHVEAQIDPQDSENSAFQPSLGFVIGILGVMFLLTFVLLLYAKFCHR 62

Query: 63  RSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFE 122
           RS     + V H  QIRSSS     GIDKTVIESLPFFRFSTLKGSK GLECA+CLSKFE
Sbjct: 63  RSVR--GDSVNHPAQIRSSSRHS--GIDKTVIESLPFFRFSTLKGSKEGLECAVCLSKFE 122

Query: 123 DIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSE 182
           DIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+LFT+SSSMR L SN SE
Sbjct: 123 DIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLFTSSSSMRLLLSNQSE 182

Query: 183 LKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGD 242
           LKQDSNIELFVQREEE+E  Q+Q+QQ HGS RFSIGRSFRK+LKS+K+ EMLIQK  DGD
Sbjct: 183 LKQDSNIELFVQREEEQE--QEQEQQPHGSSRFSIGRSFRKILKSEKDEEMLIQKACDGD 242

Query: 243 KNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEESTL 302
           +NMKNLHR NHKII+SDFVFMNRWSNVSSSD+MFLNKEMI SISS RFTS ET +E+ST 
Sbjct: 243 ENMKNLHRLNHKIIVSDFVFMNRWSNVSSSDLMFLNKEMIGSISSSRFTSFETDIEQSTA 302

Query: 303 SRSVENEE---------------------------PLKGFPSTSDCSA------RITSTE 362
            RS++NEE                           P+  FPSTS+ +A      RI S +
Sbjct: 303 PRSMQNEEILKIKEEMEIKRSFDSKFSTISQSNSVPILVFPSTSESAAKPCQTARINSPD 362

Query: 363 ARRSVSEITGVTRFGGDVHMNFNRKIEMGESSELENNVK----RQTWYPIARKTVQWFAN 397
            RRSVSEITG++RF GD+ + FN ++E GE S+L NN K    RQ WYPIA++TVQWFAN
Sbjct: 363 ERRSVSEITGISRFWGDLDLKFNTRVETGEPSDLGNNGKQERMRQNWYPIAKRTVQWFAN 422

BLAST of Cp4.1LG04g10160 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 4.0e-85
Identity = 187/396 (47.22%), Postives = 248/396 (62.63%), Query Frame = 0

Query: 31  SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRSSVHVNNGVTHGGQIR--------SSS 90
           +F+P+L  V G+L +MF LTFVLLVYAK CH        +   H  ++R        ++S
Sbjct: 32  NFEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTAS 91

Query: 91  STPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRLLPKCKHAFHINCI 150
           S    G+DKT IESLP FRFS LKGSK+GL+C++CLSKFE +EILRLLPKC+HAFHI CI
Sbjct: 92  SDRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCI 151

Query: 151 DHWLERHATCPLCRQGVS-SEDLQLFTNSSSMRFLWSNLSELKQDSNIELFVQREEEEEE 210
           D WLE+HATCPLCR  VS  ED  + TN +S RFL  N SE+++DS++EL+++REEEEE 
Sbjct: 152 DQWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEE- 211

Query: 211 HQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGDKNMKNLHRHNHKIIISDFV 270
            +  ++++ GS RFSIG SFRK+LK   + + L+ +  +     K +H+ NH+I++SD V
Sbjct: 212 -RIHREELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDVV 271

Query: 271 FMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLE--------------TYLEESTLSRSVE 330
           F NRWSNVSSSD+MFLN EM++SISS RF+SL+                 EE    R +E
Sbjct: 272 FKNRWSNVSSSDLMFLNSEMVNSISSERFSSLDHVKRGDEEDQIGILRIKEEMEAKRMLE 331

Query: 331 NE--EPLKGFPS----TSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGESS 390
           N+       F S    +   S  +     RRSVS+IT V R    +H + +       +S
Sbjct: 332 NKLTSMTTMFSSENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGS-AAETAS 391

Query: 391 ELEN------NVKRQTWYPIARKTVQWFANREKTRQ 392
            L+N        +R+ W PIARKT QWFANREK  Q
Sbjct: 392 ALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Cp4.1LG04g10160 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-71
Identity = 171/403 (42.43%), Postives = 243/403 (60.30%), Query Frame = 0

Query: 7   VTFCFFSVLF--YVQAQ---PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCH 66
           ++  FF ++F  YV AQ   P +  A +  F+P+L  + G+  ++F LTFVLLVYAK  H
Sbjct: 7   ISILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH 66

Query: 67  NRSSVHVNNGVTHGGQIRS--------SSSTPILGIDKTVIESLPFFRFSTLKGSKRGLE 126
           N      +   + G +IR         + S+   G+DK  IESLPFFRFS LKG K+GLE
Sbjct: 67  NDLRSETD---SDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLE 126

Query: 127 CAICLSKFEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSE-DLQLFTNSSS 186
           C++CLSKFED+EILRLLPKC+HAFHI CID WLE+HATCPLCR  V+ E DL +  NSS+
Sbjct: 127 CSVCLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSST 186

Query: 187 MRFLWSNLSELKQDSNIELFVQREEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGE 246
              + +     ++DS +E++++REE   +         GS RFS   SFRK+LK      
Sbjct: 187 SLRILNQSETREEDSRLEIYIEREEGTND---------GSSRFS---SFRKILKK----S 246

Query: 247 MLIQKDYDGDKNM---KNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGR 306
           +L+++  +G++N+   K +H+ NH+I++SD VF NRWSN++SSD+ FL  EM++S+SS R
Sbjct: 247 LLLER--EGNENIDEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDR 306

Query: 307 FTSLETYLEESTLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTR---FGGD 366
           F+S++  +    L R  E+ E  +      D S        RR+VSEIT V+R    GG 
Sbjct: 307 FSSVDR-VHRGNL-RDKEDMEMKRMLIKHKDSS--------RRTVSEITTVSREKAVGGS 366

Query: 367 VHMNFNRKIEMGESSELENNVKRQTWYPIARKTVQWFANREKT 390
              +     +    +      +R+ W PIAR+T QWF NREK+
Sbjct: 367 YRGSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNREKS 378

BLAST of Cp4.1LG04g10160 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-36
Identity = 110/303 (36.30%), Postives = 150/303 (49.50%), Query Frame = 0

Query: 34  PNLGFVIGILGVMFVLTFVLLVYAKYCHNRS-SVHVNN-----------GVTHGGQIRSS 93
           P +  VI +L   F LTF+LL+Y K+C  R+ SV+VN+           G  +GG +   
Sbjct: 2   PGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGG 61

Query: 94  SSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRLLPKCKHAFHINC 153
                 GID++VIESLP FRF  L G K GLECA+CL++FE  E+LRLLPKCKHAFH+ C
Sbjct: 62  RKNS--GIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVEC 121

Query: 154 IDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSELKQDSN------------- 213
           +D WL+ H+TCPLCR  V  ED+ L  + +S    W  L   K +SN             
Sbjct: 122 VDTWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDESNSVNNNPPGLTRFI 181

Query: 214 -IELFVQREEEEEEHQQQQQQIHGSFRF-SIGRSFRKMLKSD------------------ 273
            +     R     E   +  +I  S  + S   SFR+ L S                   
Sbjct: 182 PVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNC 241

Query: 274 ------KEGEMLI--QKDYDGDKNMKNLHRHNHKIIISDFVFMN-RWSNVSSSDMMFLNK 283
                 K+G +LI  ++ ++G        R  H+IIIS     + RWS V  SD+++L  
Sbjct: 242 LDRLQRKDGLLLIPNRESFEG--------RFEHRIIISGGNRDDQRWSEVRPSDLLYLRS 290

BLAST of Cp4.1LG04g10160 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-24
Identity = 60/148 (40.54%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 22  PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC----HNRSSVHVNNGVTHGGQ 81
           PS  D +     P+   V  +L  +F LT +L VY ++C     + S+ +  N    G  
Sbjct: 33  PSPPDLQT-GHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSS 92

Query: 82  IRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRG---LECAICLSKFEDIEILRLLPKCK 141
            R        G+D  V+ES P F +S++K SK G   LECAICL++ ED E +RLLP C 
Sbjct: 93  RRG-------GLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICN 152

Query: 142 HAFHINCIDHWLERHATCPLCRQGVSSE 163
           H FHI+CID WL  HATCP+CR  ++++
Sbjct: 153 HLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Cp4.1LG04g10160 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 4.4e-23
Identity = 57/151 (37.75%), Postives = 90/151 (59.60%), Query Frame = 0

Query: 16  FYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC--HNRSSVHVNNGVT 75
           F++    SS  +   SF P L  +IGIL    +L     + +KYC  H+++S      + 
Sbjct: 41  FFLDDDSSSSSS---SFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLN 100

Query: 76  HGGQIRSSSSTPIL----GIDKTVIESLPFFRFSTLKGSKRGLECAICLSKFEDIEILRL 135
           H G+   SS+  I     G+++++I+S+  +++ +  G   G +C++CLS+FE+ E LRL
Sbjct: 101 HNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRL 160

Query: 136 LPKCKHAFHINCIDHWLERHATCPLCRQGVS 161
           LPKC HAFH+ CID WL+ H+ CPLCR  V+
Sbjct: 161 LPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XF855.7e-8447.22E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ... [more]
Q9SL781.6e-7042.43Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
Q5EAE91.5e-3635.96RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q8W5717.3e-2340.54RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Q9LF646.2e-2237.75RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023531707.16.66e-285100.00E3 ubiquitin-protein ligase ATL42-like [Cucurbita pepo subsp. pepo][more]
KAG7021875.18.64e-27998.24E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022929946.11.08e-27797.99E3 ubiquitin-protein ligase ATL42-like [Cucurbita moschata][more]
XP_023006562.13.13e-27697.73E3 ubiquitin-protein ligase ATL42-like [Cucurbita maxima][more]
KAG6587983.17.44e-27698.24E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1EPJ85.21e-27897.99E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KW641.51e-27697.73E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita maxima OX=3661 GN=LOC1114992... [more]
A0A5D3DZ482.86e-18467.72E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHK32.86e-18467.72E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500995 P... [more]
A0A6J1CUL64.37e-18366.90E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
AT4G28890.14.0e-8547.22RING/U-box superfamily protein [more]
AT2G20030.11.1e-7142.43RING/U-box superfamily protein [more]
AT5G05810.11.6e-3636.30RING/U-box superfamily protein [more]
AT4G40070.15.2e-2440.54RING/U-box superfamily protein [more]
AT5G17600.14.4e-2337.75RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 188..208
NoneNo IPR availablePANTHERPTHR46539E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 21..391
NoneNo IPR availablePANTHERPTHR46539:SF1E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 21..391
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 113..156
e-value: 1.59503E-21
score: 84.6609
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 99..161
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 114..155
e-value: 6.7E-7
score: 38.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 113..156
e-value: 1.0E-12
score: 48.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 114..156
score: 12.740079
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 88..165
e-value: 1.3E-22
score: 80.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g10160.1Cp4.1LG04g10160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane