Cp4.1LG04g08030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g08030
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	abscisic acid 8'-hydroxylase 4-like
Location	Cp4.1LG04: 3474111 .. 3476373 (-)
RNA-Seq Expression	Cp4.1LG04g08030
Synteny	Cp4.1LG04g08030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATTCTCTCTTTCACTCTGTATTTAATAATATTCCTCATTTCTTCTGCTTTTTTGCTTTATTTGAAGAGAAATGAAACCCCAAAACCCAGAAAATCTCTTAGGCTTCCTCCTGGTTCCATGGGATTGCCATGGATTGGAGAAACTTTGCAGCTTTATTCTCAAGATCCCAACATATTTTTCTCCGAAAAACAGAGAAGGTAAAAAAAAAACACCAAAAAAATGATCTTTTTATGAATTTTATCGACCAAACAAGTGAAATAACTCGAGATTTTAATTGGGTTTTGTTTCTTTTCATGTTTTTTTGTGGCAGATATGGAGAAATTTTTAAAACCAACATTTTGGGATGTCCTTGTGTTATGTTGGCCAGCGCCGCCGCAGCCCGGTTCGTTTTGGTAACTAATGCTCATTTATTCCGGCCAACTTACCCCAAAAGCAAAGAGACAATGATCGGTCCGGCGGCGCTTTTCTTCCACCAGGGAAATTACCATTCTAACATTCGGAAACTTGTTCAAAATTCCCTCTGTCTCGGAAGCCTCAGAAACCTTGTTCCTCATATTGAAACCGCCGCCATTTCCGCCGCCGAATCATGGGCTGCCGCCGGCCACGTCATCAACACCTTCCTTGAAATGAAAAAGGTCCGTAACATTTCTCAAAATAAACACGAAATTATTCATAATTTTATCTAATCTCTGTTTTTTTTTTTCTGTTAAAACAGTTTTCTTTCGATGTGGGAATTCTAGCAGTGTTTGGGAAGCTAGAAAGTGATTACAGTGAGAAACTCAAAGAAAACTATTGCATATTAGACAAAGGCTACAACTCTTTTCCAACCAGATTACCAGGAACAGCATATTCCAAAGCATTATCAGTAAGAACAATGAAAAAAAAGTTAAAAAAAATCCAATCTTTTTCAAAAAAGACACGAACTTTATAAATTTCGGAGATACCCAGAATGTTCTTTTGACATATTTGATTGATTTTACAGGCAAGAAAGAAACTGAGAGAGATTCTGGGAGAGATAATAATGGAGCGGAGAGAAAAAAGGGTAACAGAGAGAGATTTGGTGGGTCATCTCTTGAGCTTCAGAGATGAAAATGGTGGAATTTTAAGTGAGGATCAGATCGCCGACAACATCATCGGAGTTCTGTTTGCGGCGCAGGACACCACCGCCAGTGTGCTGACGTGGATCCTTAAATACCTCCATGATAATTTGAAGCTCTTTGAGGCTGTCCGGGTAAGGAATTATACAAATATTAGTTACTCAATTATCCATTTAATTGGAATTTTGAAGCAAAACGTCGTCGTTTTTTAATAGGCAGAGCAGATGGAGATTCATAGAAGAAATGATGATGGGAAGATGCCTCTGTCTTGGGCACAGATTAAGGATATGCCCTTAACTCATAGGGTATGTTTGTTTTCGAGCCATCCCGGTTCAATTTTTCTGAAGATTTGAACTTTCGATCTCATGCACGTGGTTTATTTTCAGGTTGTATTAGAAAGCTTGAGGATGGCTAGTATCATATCCTTCACTTTTAGGGAGGCAGTGGTGGATGTGGAATATAAAGGTATATTCCAAGGGCTTTTTGGTCATTTCACATAATTTTTTATTTTTATTTTAAAAATTGAATTAAAATAAATAACTAATAAATATTAAATATTAAATAATTTAGGGTTCTTAATACCAAAGGGTTGGAAGGTGATGCCTTTGTTCAGGAATATTCATCACAGTCCTGACTACTTCCCAAACCCTCAAATCTTTGATCCCTCTAGATTTGAGGTACGCTTTGAATATTCATATTTTTCTCTTTTATTTTAATTCAAAAAATAATATTAAAGCATTTTTATTGTGGATTATCCAGGTGTCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACACTCTTGCCCTGGGAATGAACTTGCCAAGCTCGAGATCCTCGTCTTACTTCATCACCTCATTACCAAATTTAGGTAAATCCCCCCATTGTTTTACGATAATAAAAAGAAATAAAAAAATTAATCATATCTTTTCTTTAATCAATTGATCTATGTTCAAATTGGTAATTATGAGTCGAAATATTTAATAAATGGGAAAATAAAAGGATTTGTTACGTTTGCAGGTGGGAAGTTGTGGGATCACAAAGCGGGGTTCAATATGGGCCATTCCCAGTGCCTGTCAACGGGCTACCGGCCAGATTTTTCAAACAAGGGACAACCCACCAATAGCCTCTCATTTCTCTTTCAATATACTTT

mRNA sequence

ATGGAGATTCTCTCTTTCACTCTGTATTTAATAATATTCCTCATTTCTTCTGCTTTTTTGCTTTATTTGAAGAGAAATGAAACCCCAAAACCCAGAAAATCTCTTAGGCTTCCTCCTGGTTCCATGGGATTGCCATGGATTGGAGAAACTTTGCAGCTTTATTCTCAAGATCCCAACATATTTTTCTCCGAAAAACAGAGAAGATATGGAGAAATTTTTAAAACCAACATTTTGGGATGTCCTTGTGTTATGTTGGCCAGCGCCGCCGCAGCCCGGTTCGTTTTGGTAACTAATGCTCATTTATTCCGGCCAACTTACCCCAAAAGCAAAGAGACAATGATCGGTCCGGCGGCGCTTTTCTTCCACCAGGGAAATTACCATTCTAACATTCGGAAACTTGTTCAAAATTCCCTCTGTCTCGGAAGCCTCAGAAACCTTGTTCCTCATATTGAAACCGCCGCCATTTCCGCCGCCGAATCATGGGCTGCCGCCGGCCACGTCATCAACACCTTCCTTGAAATGAAAAAGTTTTCTTTCGATGTGGGAATTCTAGCAGTGTTTGGGAAGCTAGAAAGTGATTACAGTGAGAAACTCAAAGAAAACTATTGCATATTAGACAAAGGCTACAACTCTTTTCCAACCAGATTACCAGGAACAGCATATTCCAAAGCATTATCAGCAAGAAAGAAACTGAGAGAGATTCTGGGAGAGATAATAATGGAGCGGAGAGAAAAAAGGGTAACAGAGAGAGATTTGGTGGGTCATCTCTTGAGCTTCAGAGATGAAAATGGTGGAATTTTAAGTGAGGATCAGATCGCCGACAACATCATCGGAGTTCTGTTTGCGGCGCAGGACACCACCGCCAGTGTGCTGACGTGGATCCTTAAATACCTCCATGATAATTTGAAGCTCTTTGAGGCTGTCCGGGCAGAGCAGATGGAGATTCATAGAAGAAATGATGATGGGAAGATGCCTCTGTCTTGGGCACAGATTAAGGATATGCCCTTAACTCATAGGGTTGTATTAGAAAGCTTGAGGATGGCTAGTATCATATCCTTCACTTTTAGGGAGGCAGTGGTGGATGTGGAATATAAAGGGTTCTTAATACCAAAGGGTTGGAAGGTGATGCCTTTGTTCAGGAATATTCATCACAGTCCTGACTACTTCCCAAACCCTCAAATCTTTGATCCCTCTAGATTTGAGGTGTCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACACTCTTGCCCTGGGAATGAACTTGCCAAGCTCGAGATCCTCGTCTTACTTCATCACCTCATTACCAAATTTAGGTGGGAAGTTGTGGGATCACAAAGCGGGGTTCAATATGGGCCATTCCCAGTGCCTGTCAACGGGCTACCGGCCAGATTTTTCAAACAAGGGACAACCCACCAATAGCCTCTCATTTCTCTTTCAATATACTTT

Coding sequence (CDS)

ATGGAGATTCTCTCTTTCACTCTGTATTTAATAATATTCCTCATTTCTTCTGCTTTTTTGCTTTATTTGAAGAGAAATGAAACCCCAAAACCCAGAAAATCTCTTAGGCTTCCTCCTGGTTCCATGGGATTGCCATGGATTGGAGAAACTTTGCAGCTTTATTCTCAAGATCCCAACATATTTTTCTCCGAAAAACAGAGAAGATATGGAGAAATTTTTAAAACCAACATTTTGGGATGTCCTTGTGTTATGTTGGCCAGCGCCGCCGCAGCCCGGTTCGTTTTGGTAACTAATGCTCATTTATTCCGGCCAACTTACCCCAAAAGCAAAGAGACAATGATCGGTCCGGCGGCGCTTTTCTTCCACCAGGGAAATTACCATTCTAACATTCGGAAACTTGTTCAAAATTCCCTCTGTCTCGGAAGCCTCAGAAACCTTGTTCCTCATATTGAAACCGCCGCCATTTCCGCCGCCGAATCATGGGCTGCCGCCGGCCACGTCATCAACACCTTCCTTGAAATGAAAAAGTTTTCTTTCGATGTGGGAATTCTAGCAGTGTTTGGGAAGCTAGAAAGTGATTACAGTGAGAAACTCAAAGAAAACTATTGCATATTAGACAAAGGCTACAACTCTTTTCCAACCAGATTACCAGGAACAGCATATTCCAAAGCATTATCAGCAAGAAAGAAACTGAGAGAGATTCTGGGAGAGATAATAATGGAGCGGAGAGAAAAAAGGGTAACAGAGAGAGATTTGGTGGGTCATCTCTTGAGCTTCAGAGATGAAAATGGTGGAATTTTAAGTGAGGATCAGATCGCCGACAACATCATCGGAGTTCTGTTTGCGGCGCAGGACACCACCGCCAGTGTGCTGACGTGGATCCTTAAATACCTCCATGATAATTTGAAGCTCTTTGAGGCTGTCCGGGCAGAGCAGATGGAGATTCATAGAAGAAATGATGATGGGAAGATGCCTCTGTCTTGGGCACAGATTAAGGATATGCCCTTAACTCATAGGGTTGTATTAGAAAGCTTGAGGATGGCTAGTATCATATCCTTCACTTTTAGGGAGGCAGTGGTGGATGTGGAATATAAAGGGTTCTTAATACCAAAGGGTTGGAAGGTGATGCCTTTGTTCAGGAATATTCATCACAGTCCTGACTACTTCCCAAACCCTCAAATCTTTGATCCCTCTAGATTTGAGGTGTCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACACTCTTGCCCTGGGAATGAACTTGCCAAGCTCGAGATCCTCGTCTTACTTCATCACCTCATTACCAAATTTAGGTGGGAAGTTGTGGGATCACAAAGCGGGGTTCAATATGGGCCATTCCCAGTGCCTGTCAACGGGCTACCGGCCAGATTTTTCAAACAAGGGACAACCCACCAATAG

Protein sequence

MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ

Homology

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 651.4 bits (1679), Expect = 8.1e-186
Identity = 307/459 (66.88%), Postives = 378/459 (82.35%), Query Frame = 0


Query: 9   YLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQRR 68
           +L++ ++    LL        K     +LPPGSMG P++GETLQLYSQ+PN+FF+ KQ+R
Sbjct: 6   FLVVPILILCLLLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSKQKR 65

Query: 69  YGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHS 128
           YGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+ALFFHQG+YHS
Sbjct: 66  YGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHS 125

Query: 129 NIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFG 188
           +IRKLVQ+S    ++R L+P IE  A+S+ +SWA    +++T+ EMKKF+FDVGILA+FG
Sbjct: 126 HIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILAIFG 185

Query: 189 KLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVT 248
            LES Y E LK NY I+DKGYNSFP  LPGT+Y KAL ARK+L+ I+ EII ERREKR  
Sbjct: 186 HLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRAL 245

Query: 249 ERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAV 308
           + D +GHLL+F++E G +L+++QIADNIIGVLFAAQDTTAS LTWILKYLHD+ KL EAV
Sbjct: 246 QTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAV 305

Query: 309 RAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFL 368
           +AEQ  I+  N   K PL+W Q ++MPLTH+V++ESLRMASIISFTFREAVVDVEYKG+L
Sbjct: 306 KAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYL 365

Query: 369 IPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAKLE 428
           IPKGWKVMPLFRNIHH+P YF NP++FDPSRFEV+P+PNTFMPFG+GVH+CPGNELAKL+
Sbjct: 366 IPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQ 425

Query: 429 ILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARF 468
           IL+ LHHL++ FRWEV G + G+QY PFP+P NGLPA F
Sbjct: 426 ILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.4e-182
Identity = 310/469 (66.10%), Postives = 378/469 (80.60%), Query Frame = 0


Query: 8   LYLIIFLIS--SAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEK 67
           LY+ +F++   S +  + K N +   + + +LPPGSMG P+IGETLQLYSQDPN FF  +
Sbjct: 9   LYISMFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFFINR 68

Query: 68  QRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGN 127
           QRR+GEIFKT ILGCPCVMLAS  AARFVLV  A+LF+PTYPKSKE +IG +A+FFHQG+
Sbjct: 69  QRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQGD 128

Query: 128 YHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILA 187
           YH+++RKLVQ  L   S+RN +P+IE  +ISA  SW   GHV+NT+ EMKKFSF+VGILA
Sbjct: 129 YHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSW-VGGHVVNTYHEMKKFSFEVGILA 188

Query: 188 VFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREK 247
           +FG L+    E+LK+NY I+DKGYNSFP  LPGT Y KAL ARKKL +IL EII E +EK
Sbjct: 189 IFGHLDGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKEK 248

Query: 248 RVTERDLVGHLLSFRDENGG-ILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKL 307
           +  E+ L+   L+ ++E G  +L+EDQIADNIIGVLFAAQDTTASVLTWI+KYLHDN KL
Sbjct: 249 KTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDNPKL 308

Query: 308 FEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEY 367
            E V+AEQ  I + N+     L+W Q + MP+T RVVLE+LRMASIISF FREAV DVEY
Sbjct: 309 LECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVADVEY 368

Query: 368 KGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNEL 427
           KG+LIPKGWKVMPLFRNIHH+P++FP+PQ FDPSRFE +P+PNTFMPFG+GVH+CPGNEL
Sbjct: 369 KGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPGNEL 428

Query: 428 AKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTT 474
           AKLEIL++ HHL+TKFRWEVVGS SG+QYGPFPVP+ GL ARF+K  +T
Sbjct: 429 AKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFWKTTST 476

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 2.6e-176
Identity = 304/475 (64.00%), Postives = 375/475 (78.95%), Query Frame = 0


Query: 5   SFTLYLII-FLISSAFLLYLKRNETPKPR-------KSLRLPPGSMGLPWIGETLQLYSQ 64
           SF + ++I F IS AF+ Y+      + +       K++RLPPGSMG P+IGETLQLYSQ
Sbjct: 4   SFVIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQ 63

Query: 65  DPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGP 124
           DPN+FF+ KQ+RYGEIFKT+ILGCPCVMLAS  AARFVLVT AHLF+PTYP+SKE MIGP
Sbjct: 64  DPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGP 123

Query: 125 AALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKK 184
           +ALFFHQG+YH  +RKLVQ  L   +LR LVP +E A  S   SW   G+V +TF  MK+
Sbjct: 124 SALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW--DGNVSSTFHAMKR 183

Query: 185 FSFDVGILAVF-GKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREIL 244
            SFDVGI+ +F G+L+     +L++NY I++KGYNSFP   PGT Y KA+ AR++L  +L
Sbjct: 184 LSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVL 243

Query: 245 GEIIMERREKRVTERDLVGHLLSFR-DENGGILSEDQIADNIIGVLFAAQDTTASVLTWI 304
            +I+ ERR +     DL+G L+  R  ++G +L+++Q+ADNIIGVLFAAQDTTASVLTWI
Sbjct: 244 SDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWI 303

Query: 305 LKYLHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFT 364
           +KYLHD+ KL EAVRAEQ  I   ND G++PL+WAQ + M LTH+V+LESLRMASIISFT
Sbjct: 304 VKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFT 363

Query: 365 FREAVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGN 424
           FREAV DVEYKGFLIPKGWKVMPLFRNIHH+PDYF +PQ FDPSRF+VSPRPNTFMPFGN
Sbjct: 364 FREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGN 423

Query: 425 GVHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFK 470
           GVH+CPGNELAKLE+LVL+HHL+T +RWE+VGS   V+Y PFPVP +GL A+ ++
Sbjct: 424 GVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWR 476

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 4.4e-176
Identity = 304/478 (63.60%), Postives = 376/478 (78.66%), Query Frame = 0


Query: 5   SFTLYLII-FLISSAFLLYLKRNETPKPR-------KSLRLPPGSMGLPWIGETLQLYSQ 64
           SF + ++I F IS AF+ Y+      + +       K++RLPPGSMG P+IGETLQLYSQ
Sbjct: 4   SFVIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQ 63

Query: 65  DPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGP 124
           DPN+FF+ KQ+RYGEIFKT+ILGCPCVMLAS  AARFVLVT AHLF+PTYP+SKE MIGP
Sbjct: 64  DPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGP 123

Query: 125 AALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKK 184
           +ALFF+QG+YH  +RKLVQ  L   +LR LVP +E A  S   SW   G+V +TF  MK+
Sbjct: 124 SALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW--DGNVSSTFHAMKR 183

Query: 185 FSFDVGILAVF-GKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREIL 244
            SFDVGI+ +F G+L+     +L++NY I++KGYNSFP   PGT Y KA+ AR++L  +L
Sbjct: 184 LSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVL 243

Query: 245 GEIIMERREKRVTERDLVGHLLSFR-DENGGILSEDQIADNIIGVLFAAQDTTASVLTWI 304
            +I+ ERR +     DL+G L+  R  ++G +L+++Q+ADNIIGVLFAAQDTTASVLTWI
Sbjct: 244 SDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWI 303

Query: 305 LKYLHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFT 364
           +KYLHD+ KL EAVRAEQ  I   ND G++PL+WAQ + M LTH+V+LESLRMASIISFT
Sbjct: 304 VKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFT 363

Query: 365 FREAVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGN 424
           FREAV DVEYKGFLIPKGWKVMPLFRNIHH+PDYF +PQ FDPSRF+VSPRPNTFMPFGN
Sbjct: 364 FREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGN 423

Query: 425 GVHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGT 473
           GVH+CPGNELAKLE+LVL+HHL+T +RWE+VGS   V+Y PFPVP +GL A+ ++  T
Sbjct: 424 GVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWRDDT 479

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.7e-173
Identity = 290/444 (65.32%), Postives = 357/444 (80.41%), Query Frame = 0


Query: 32  RKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAA 91
           +K LRLPPGSMG P++GETLQLYSQDPN+FF+ KQ+RYGEIFKTN+LGCPCVMLAS  AA
Sbjct: 46  KKKLRLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPEAA 105

Query: 92  RFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIE 151
           RFVLV+ A LF+PTYP SKE MIGP+ALFFHQG YH  +R+LVQ +L   SLR LVP ++
Sbjct: 106 RFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPDVD 165

Query: 152 TAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVF-GKLESDYSEKLKENYCILDKGYN 211
            A  +   +W + GHV +TF  MKK SFDVG++ +F G+L   + E+L+ NY ++++GYN
Sbjct: 166 AAVAATLAAW-SGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERGYN 225

Query: 212 SFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLSFRDENG----GI 271
            FP R PGT Y KA+ ARK+LR IL EI+ ERR +     DL+G L+  RD+       +
Sbjct: 226 CFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDGTAGAVAL 285

Query: 272 LSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAEQMEIHRRNDDGKMPL 331
           L++DQIADN++GVLFAAQDTTASVLTWILKYLHD+ KL EAV+AEQM I+  N+ GK PL
Sbjct: 286 LTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKRPL 345

Query: 332 SWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPKGWKVMPLFRNIHHSP 391
           +W Q + M LTH+V+LESLRMASIISFTFREAV DVEYKGFLIPKGWKVMPLFRNIHH+P
Sbjct: 346 TWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNP 405

Query: 392 DYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAKLEILVLLHHLITKFRWEVVG 451
           DYF +PQ FDPSRF+V+PRP+TF+PFG+GVH+CPGNELAKLE+LVL+H L+T +RWE+VG
Sbjct: 406 DYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWEIVG 465

Query: 452 SQSGVQYGPFPVPVNGLPARFFKQ 471
           +   V+Y PFPVP  GL A+ +KQ
Sbjct: 466 ASDEVEYSPFPVPRGGLNAKLWKQ 488

[Close]

BLAST of Cp4.1LG04g08030 vs. NCBI nr
Match: XP_023530098.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ
Sbjct: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. NCBI nr
Match: XP_022927667.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata])

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 466/475 (98.11%), Postives = 471/475 (99.16%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIF ISSAFLLY KRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFFISSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQMEI+RRN DGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMEIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHH+PDYFPNPQIFDPSRFEV+PRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFP+PVNGLPARFFKQ TT+Q
Sbjct: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. NCBI nr
Match: XP_022988854.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita maxima])

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 464/475 (97.68%), Postives = 471/475 (99.16%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIFLISSAFLLY KRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFLISSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKF+FD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFAFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQM+I+RRN DGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMDIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEV+PRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEIL+LLHHL+TKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQ T +Q
Sbjct: 421 GNELAKLEILILLHHLLTKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQTTANQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. NCBI nr
Match: KAG7022447.1 (Abscisic acid 8'-hydroxylase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 464/475 (97.68%), Postives = 470/475 (98.95%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIFLISSAF LY KRNET KPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFLISSAFWLYFKRNETTKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSE+QIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSENQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQMEI+RRN DGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMEIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHH+PDYFPNPQIFDPSRFEV+PRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEILVLLHHLITKFRWEVVGSQSG+QYGPFPVPVNGLPARFFKQ TT+Q
Sbjct: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGIQYGPFPVPVNGLPARFFKQTTTNQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. NCBI nr
Match: XP_038887333.1 (abscisic acid 8'-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 838 bits (2164), Expect = 2.13e-304
Identity = 413/482 (85.68%), Postives = 446/482 (92.53%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLL-------YLKRNETPKPRKSLRLPPGSMGLPWIGETLQL 60
           ME LS TLY+II +I   FL        +LKRN+ PK +K L+LPPGSMGLPWIGETLQL
Sbjct: 1   MEALSVTLYIIIIIIIFLFLFAFFFSISFLKRNQKPKSQKVLKLPPGSMGLPWIGETLQL 60

Query: 61  YSQDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETM 120
           YSQDPNIFFS+KQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETM
Sbjct: 61  YSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETM 120

Query: 121 IGPAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLE 180
           IGPAALFFHQGNYHSN+RKLVQNSL L  LR LVPHIE AAISA +SWAAAGHVINTFLE
Sbjct: 121 IGPAALFFHQGNYHSNLRKLVQNSLSLERLRILVPHIEAAAISATDSWAAAGHVINTFLE 180

Query: 181 MKKFSFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLRE 240
           MKK+SF+VGI+AVF KLE++Y EKLKE Y ILDKGYN FPTRLPGTAYSKALSARKKLRE
Sbjct: 181 MKKYSFEVGIVAVFEKLENEYKEKLKEKYSILDKGYNCFPTRLPGTAYSKALSARKKLRE 240

Query: 241 ILGEIIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTW 300
           ILGEIIMERREKRVTERDLVGHLL+FRDENG  LSEDQIADNIIGVLFAAQDTTA+ LTW
Sbjct: 241 ILGEIIMERREKRVTERDLVGHLLNFRDENGETLSEDQIADNIIGVLFAAQDTTATALTW 300

Query: 301 ILKYLHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISF 360
           ILK+LHDNLKLFEAV+AEQMEI+RRN DGKMPLSWAQIKDMP THRVVLESLRMASIISF
Sbjct: 301 ILKFLHDNLKLFEAVKAEQMEIYRRNGDGKMPLSWAQIKDMPFTHRVVLESLRMASIISF 360

Query: 361 TFREAVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFG 420
           TFREAVVD+EYKG+LIPKGWKVMPLFRNIHH+P+YFP+P IFDPSRFE++P+PNTFMPFG
Sbjct: 361 TFREAVVDMEYKGYLIPKGWKVMPLFRNIHHNPEYFPDPHIFDPSRFELAPKPNTFMPFG 420

Query: 421 NGVHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTT 475
           NGVHSCPGNELAKLEIL+LLHHLITKFRWEVVGS+SGV+YGPFP+PV+GLPARFFK  TT
Sbjct: 421 NGVHSCPGNELAKLEILILLHHLITKFRWEVVGSESGVEYGPFPMPVHGLPARFFKHTTT 480

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL
Match: A0A6J1EHU3 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111434488 PE=3 SV=1)

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 466/475 (98.11%), Postives = 471/475 (99.16%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIF ISSAFLLY KRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFFISSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQMEI+RRN DGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMEIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHH+PDYFPNPQIFDPSRFEV+PRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFP+PVNGLPARFFKQ TT+Q
Sbjct: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL
Match: A0A6J1JMR7 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486079 PE=3 SV=1)

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 464/475 (97.68%), Postives = 471/475 (99.16%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60
           MEILSFTLYLIIFLISSAFLLY KRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI
Sbjct: 1   MEILSFTLYLIIFLISSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNI 60

Query: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120
           FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF
Sbjct: 61  FFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALF 120

Query: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFD 180
           FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKF+FD
Sbjct: 121 FHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFAFD 180

Query: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240
           VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM
Sbjct: 181 VGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIM 240

Query: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300
           ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD
Sbjct: 241 ERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHD 300

Query: 301 NLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360
           NLKLFEAVRAEQM+I+RRN DGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV
Sbjct: 301 NLKLFEAVRAEQMDIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVV 360

Query: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCP 420
           DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEV+PRPNTFMPFGNGVHSCP
Sbjct: 361 DVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCP 420

Query: 421 GNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           GNELAKLEIL+LLHHL+TKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQ T +Q
Sbjct: 421 GNELAKLEILILLHHLLTKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQTTANQ 475

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL
Match: A0A1S3BZQ3 (abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1)

HSP 1 Score: 818 bits (2112), Expect = 7.98e-297
Identity = 405/480 (84.38%), Postives = 437/480 (91.04%), Query Frame = 0


Query: 1   MEILSFTLY---LIIFLISSAFLLYLKR--NETPKPRKSLRLPPGSMGLPWIGETLQLYS 60
           ME LS  LY   L +FL +   + +LKR  NE  K + SL+LPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMK 180
           PAALFFHQGNYHSN+RKLV NSL L  LR LVP IE AAISA +SWAAAGHVINTF EMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180

Query: 181 KFSFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREIL 240
           K+SF+VGI+AVFGKLE++Y EKLK+ YCILDKGYN FPTRLPGT YSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWIL 300
           GEIIMERREKRVTERD VGHLL++RDENG  LSEDQIADNIIGVLFAAQDTTA+VLTWIL
Sbjct: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTF 360
           KYLHDN KLFEAV+AEQMEI+RRN +GKMPLSW QIKDMP THRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNG 420
           REAVVDVEYKG+LIPKGWKVMPLFRNIHH+ +YFP+P IFDPSRFEV+PRPNTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           VHSCPGNELAKLEIL+LLHHLITKFRWEV GSQSGV+YGPFP+P+ GLP+RFFKQ TT+Q
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL
Match: A0A6J1I8M8 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 PE=3 SV=1)

HSP 1 Score: 813 bits (2099), Expect = 7.07e-295
Identity = 405/478 (84.73%), Postives = 436/478 (91.21%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAF--LLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDP 60
           ME LS  LY+II L   AF  + +LKRNE PK +++L+LPPGSMGLPW GETLQLYSQDP
Sbjct: 1   MEALSVPLYIIISLFLLAFFSISFLKRNEQPKSQEALKLPPGSMGLPWFGETLQLYSQDP 60

Query: 61  NIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 120
           NIFFSEKQRR+GEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKET+IGPAA
Sbjct: 61  NIFFSEKQRRFGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETLIGPAA 120

Query: 121 LFFHQGNYHSNIRKLVQNSLCLGSLRN-LVPHIETAAISAAESWAAAGHVINTFLEMKKF 180
           LFFHQGNYHSN+RKLVQNSL L  LR  LVPHIE AAISAA+SWAA  HV+NTFL+MKKF
Sbjct: 121 LFFHQGNYHSNLRKLVQNSLSLQRLRTTLVPHIEAAAISAADSWAATAHVVNTFLQMKKF 180

Query: 181 SFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGE 240
           SF+V I+AVFGKLE+ YSEKLKENYCILDKGYNSFPTRLPGTA SKALSARKKLREIL E
Sbjct: 181 SFEVAIVAVFGKLETHYSEKLKENYCILDKGYNSFPTRLPGTACSKALSARKKLREILRE 240

Query: 241 IIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKY 300
           II+ERREK   ERD VG LLS+RDENG  LSE+QIADN+IGVLFAAQDTTASVLTWILKY
Sbjct: 241 IIVERREKEEAERDFVGQLLSYRDENGERLSEEQIADNVIGVLFAAQDTTASVLTWILKY 300

Query: 301 LHDNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFRE 360
           LHD+ KLFEAVRAEQMEI+RRN +GKMPLSWAQIKDMP THRVVLESLRMASIISFTFRE
Sbjct: 301 LHDSFKLFEAVRAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFTFRE 360

Query: 361 AVVDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVH 420
           AVVDVEYKG+ IPKGWKVMPLFRNIHH+ DYF +P  FDPSRFEV+PRPNTFMPFGNGVH
Sbjct: 361 AVVDVEYKGYRIPKGWKVMPLFRNIHHNHDYFNDPHTFDPSRFEVAPRPNTFMPFGNGVH 420

Query: 421 SCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           SCPGNELAKLEIL+LLHHL+TKFRWEVVG QSGVQYGPFPVPV+GLPARFFKQ TT+Q
Sbjct: 421 SCPGNELAKLEILILLHHLVTKFRWEVVGPQSGVQYGPFPVPVHGLPARFFKQTTTNQ 478

[Close]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL
Match: A0A6J1C2D9 (abscisic acid 8'-hydroxylase 4 OS=Momordica charantia OX=3673 GN=LOC111007825 PE=3 SV=1)

HSP 1 Score: 812 bits (2097), Expect = 1.32e-294
Identity = 403/476 (84.66%), Postives = 439/476 (92.23%), Query Frame = 0


Query: 1   MEILSFTLYLIIFLISSAFLL-YLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPN 60
           MEILS TL  I+  +S+ F L +LKR +  K +K  RLPPGSMGLPWIGETLQLYSQDPN
Sbjct: 1   MEILSVTLCTILLFLSAFFSLPFLKRMKKKKAQKIARLPPGSMGLPWIGETLQLYSQDPN 60

Query: 61  IFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAAL 120
           IFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVT+A  FRPTYPKSKETMIGPAAL
Sbjct: 61  IFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTHASFFRPTYPKSKETMIGPAAL 120

Query: 121 FFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSF 180
           FFHQGNYHS++RKLVQ+SL L  LRNLVP IE AAISAA+SWAAAG VINT+L+MKKFSF
Sbjct: 121 FFHQGNYHSHLRKLVQSSLSLERLRNLVPDIEAAAISAADSWAAAGQVINTYLQMKKFSF 180

Query: 181 DVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEII 240
            VGILAVFG+LES+YSEKLK+NYCILDKGYNSFPT+LPGTAYSKALSARK+LR+ILGEII
Sbjct: 181 QVGILAVFGQLESEYSEKLKQNYCILDKGYNSFPTKLPGTAYSKALSARKRLRKILGEII 240

Query: 241 MERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLH 300
           MERREKRV+ERDLVGHLLSFRDE G  LSED+IADNIIGVLFAAQDTTAS LTWILKYLH
Sbjct: 241 MERREKRVSERDLVGHLLSFRDEKGETLSEDEIADNIIGVLFAAQDTTASALTWILKYLH 300

Query: 301 DNLKLFEAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAV 360
           DN KLF AVRAEQMEI+R N +G++PLSWAQIKDMP THRVVLESLRMASIISFTFREAV
Sbjct: 301 DNHKLFHAVRAEQMEIYRTNCEGRIPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAV 360

Query: 361 VDVEYKGFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSC 420
           VDVEYKG+LIPKGWKVMPLFRNIHH+PDYF +P IFDPSRFEV+P+PNTFMPFGNGVH+C
Sbjct: 361 VDVEYKGYLIPKGWKVMPLFRNIHHNPDYFSDPHIFDPSRFEVAPKPNTFMPFGNGVHAC 420

Query: 421 PGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARFFKQGTTHQ 475
           PGNELAKLEIL+LLHHL+TKFRWEVVGS++GVQYGPFPVPVNGLPARF KQ T +Q
Sbjct: 421 PGNELAKLEILILLHHLVTKFRWEVVGSENGVQYGPFPVPVNGLPARFSKQITANQ 476

[Close]

BLAST of Cp4.1LG04g08030 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 651.4 bits (1679), Expect = 5.7e-187
Identity = 307/459 (66.88%), Postives = 378/459 (82.35%), Query Frame = 0


Query: 9   YLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQRR 68
           +L++ ++    LL        K     +LPPGSMG P++GETLQLYSQ+PN+FF+ KQ+R
Sbjct: 6   FLVVPILILCLLLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSKQKR 65

Query: 69  YGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHS 128
           YGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+ALFFHQG+YHS
Sbjct: 66  YGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHS 125

Query: 129 NIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFG 188
           +IRKLVQ+S    ++R L+P IE  A+S+ +SWA    +++T+ EMKKF+FDVGILA+FG
Sbjct: 126 HIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILAIFG 185

Query: 189 KLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVT 248
            LES Y E LK NY I+DKGYNSFP  LPGT+Y KAL ARK+L+ I+ EII ERREKR  
Sbjct: 186 HLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRAL 245

Query: 249 ERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAV 308
           + D +GHLL+F++E G +L+++QIADNIIGVLFAAQDTTAS LTWILKYLHD+ KL EAV
Sbjct: 246 QTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAV 305

Query: 309 RAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFL 368
           +AEQ  I+  N   K PL+W Q ++MPLTH+V++ESLRMASIISFTFREAVVDVEYKG+L
Sbjct: 306 KAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYL 365

Query: 369 IPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAKLE 428
           IPKGWKVMPLFRNIHH+P YF NP++FDPSRFEV+P+PNTFMPFG+GVH+CPGNELAKL+
Sbjct: 366 IPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQ 425

Query: 429 ILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPARF 468
           IL+ LHHL++ FRWEV G + G+QY PFP+P NGLPA F
Sbjct: 426 ILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

[Close]

BLAST of Cp4.1LG04g08030 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 551.6 bits (1420), Expect = 6.2e-157
Identity = 265/453 (58.50%), Postives = 345/453 (76.16%), Query Frame = 0


Query: 12  IFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQRRYGE 71
           +FL    F+  ++R+ + K    L LPPG+MG P++GET QLYSQDPN+FF+ KQRRYG 
Sbjct: 15  LFLCLLRFIAGVRRSSSTK----LPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGS 74

Query: 72  IFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIR 131
           +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A+FFHQG+YHS +R
Sbjct: 75  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 134

Query: 132 KLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFGKLE 191
           KLV  +    ++RN+VPHIE+ A  +  SW   G  +NT+ EMK ++F+V ++++ GK E
Sbjct: 135 KLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALISILGKDE 194

Query: 192 SDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERD 251
             Y E LK  Y IL+KGYNS P  LPGT + KA+ ARK+L +IL  I+ +RR+   +  D
Sbjct: 195 VYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTD 254

Query: 252 LVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAE 311
           L+G   SF ++  G L+++QIADNIIGV+FAA+DTTASVLTWILKYL DN  + EAV  E
Sbjct: 255 LLG---SFMEDKAG-LTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEE 314

Query: 312 QMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPK 371
           QM I +   +G+  L+W   K MPLT+RV+ E+LR A+I+SFTFREAV DVEY+G+LIPK
Sbjct: 315 QMAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPK 374

Query: 372 GWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAKLEILV 431
           GWKV+PLFRNIHH+ D F +P  FDPSRFEV+P+PNTFMPFG+G+HSCPGNELAKLEI V
Sbjct: 375 GWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISV 434

Query: 432 LLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLP 465
           L+HHL TK+RW +VG   G+QYGPF +P NGLP
Sbjct: 435 LIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLP 456

[Close]

BLAST of Cp4.1LG04g08030 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 549.3 bits (1414), Expect = 3.1e-156
Identity = 262/458 (57.21%), Postives = 343/458 (74.89%), Query Frame = 0


Query: 10  LIIFLISSAFLLYLKRNETPKPR---KSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQ 69
           L + L + +  LY  R    + R     L LPPG+MG P++GET QLYSQDPN+FF  KQ
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 70  RRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNY 129
           +RYG +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A+FFHQG+Y
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 130 HSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAV 189
           H+ +RKLV  +    S+RN+VP IE+ A  +  SW   G +INT+ EMK ++F+V +L++
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW--EGTMINTYQEMKTYTFNVALLSI 185

Query: 190 FGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKR 249
           FGK E  Y E LK  Y IL+KGYNS P  LPGT + K++ ARK+L +IL  I+ ERR+  
Sbjct: 186 FGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNG 245

Query: 250 VTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFE 309
            +  DL+G  +  ++E    L+++QIADNIIGV+FAA+DTTASV++WILKYL +N  + E
Sbjct: 246 SSHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLE 305

Query: 310 AVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKG 369
           AV  EQM I +  ++G+  L+W   K MPLT RV+ E+LR+ASI+SFTFREAV DVEY+G
Sbjct: 306 AVTEEQMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEG 365

Query: 370 FLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELAK 429
           +LIPKGWKV+PLFRNIHHS D F NP  FDPSRFEV+P+PNTFMPFGNG HSCPGNELAK
Sbjct: 366 YLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAK 425

Query: 430 LEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLP 465
           LE+ +++HHL TK+ W +VG+  G+QYGPF +P NGLP
Sbjct: 426 LEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLP 456

[Close]

BLAST of Cp4.1LG04g08030 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 529.6 bits (1363), Expect = 2.5e-150
Identity = 261/461 (56.62%), Postives = 336/461 (72.89%), Query Frame = 0


Query: 7   TLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQ 66
           TL +++ ++   F  +L   E     + LRLPPGSMGLP+IGETL+LY+++PN FF+ +Q
Sbjct: 24  TLTIVVVVVVLLFKWWLHWKE-----QRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQ 83

Query: 67  RRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNY 126
            +YG+IFKT+ILGCPCVM++S  AAR VLV+ AHLF+PTYP SKE MIGP ALFFHQG Y
Sbjct: 84  NKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPY 143

Query: 127 HSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAV 186
           HS +++LVQ+S    +LR  V HIE   +    SW +    INT   MK+++FDV I++ 
Sbjct: 144 HSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSA 203

Query: 187 FG-KLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREK 246
           FG K E    + +K  Y  L++GYNS P  LPGT + K++ AR +L E L ++I +RRE 
Sbjct: 204 FGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRREN 263

Query: 247 RVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLF 306
              E  L+G LL  +D+    LS+ QIADNIIGV+FAA DTTASVLTW+LKYLHD+  L 
Sbjct: 264 GREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLL 323

Query: 307 EAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYK 366
           + V  EQ  I ++       +SW   + MPLT RV+ E+LR AS++SFTFREAV DVEY 
Sbjct: 324 QEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 383

Query: 367 GFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELA 426
           G+LIPKGWKV+PLFR IHHS ++FP+P+ FDPSRFEV+P+P T+MPFGNGVHSCPG+ELA
Sbjct: 384 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 443

Query: 427 KLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPAR 467
           KLE+L+LLHHL T FRWEV+G + G+QYGPFPVP  GLP R
Sbjct: 444 KLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIR 478

[Close]

BLAST of Cp4.1LG04g08030 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 529.6 bits (1363), Expect = 2.5e-150
Identity = 261/461 (56.62%), Postives = 336/461 (72.89%), Query Frame = 0


Query: 7   TLYLIIFLISSAFLLYLKRNETPKPRKSLRLPPGSMGLPWIGETLQLYSQDPNIFFSEKQ 66
           TL +++ ++   F  +L   E     + LRLPPGSMGLP+IGETL+LY+++PN FF+ +Q
Sbjct: 24  TLTIVVVVVVLLFKWWLHWKE-----QRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQ 83

Query: 67  RRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNY 126
            +YG+IFKT+ILGCPCVM++S  AAR VLV+ AHLF+PTYP SKE MIGP ALFFHQG Y
Sbjct: 84  NKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPY 143

Query: 127 HSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAV 186
           HS +++LVQ+S    +LR  V HIE   +    SW +    INT   MK+++FDV I++ 
Sbjct: 144 HSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSA 203

Query: 187 FG-KLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREK 246
           FG K E    + +K  Y  L++GYNS P  LPGT + K++ AR +L E L ++I +RRE 
Sbjct: 204 FGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRREN 263

Query: 247 RVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLF 306
              E  L+G LL  +D+    LS+ QIADNIIGV+FAA DTTASVLTW+LKYLHD+  L 
Sbjct: 264 GREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLL 323

Query: 307 EAVRAEQMEIHRRNDDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYK 366
           + V  EQ  I ++       +SW   + MPLT RV+ E+LR AS++SFTFREAV DVEY 
Sbjct: 324 QEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 383

Query: 367 GFLIPKGWKVMPLFRNIHHSPDYFPNPQIFDPSRFEVSPRPNTFMPFGNGVHSCPGNELA 426
           G+LIPKGWKV+PLFR IHHS ++FP+P+ FDPSRFEV+P+P T+MPFGNGVHSCPG+ELA
Sbjct: 384 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 443

Query: 427 KLEILVLLHHLITKFRWEVVGSQSGVQYGPFPVPVNGLPAR 467
           KLE+L+LLHHL T FRWEV+G + G+QYGPFPVP  GLP R
Sbjct: 444 KLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIR 478

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g08030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJK2	8.1e-186	66.88	Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ...	[more]
A9QNE7	1.4e-182	66.10	Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
Q0J185	2.6e-176	64.00	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]
A2Z212	4.4e-176	63.60	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q09J78	2.7e-173	65.32	Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]

BLAST of Cp4.1LG04g08030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530098.1	0.0	100.00	abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927667.1	0.0	98.11	abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata]	[more]
XP_022988854.1	0.0	97.68	abscisic acid 8'-hydroxylase 4-like [Cucurbita maxima]	[more]
KAG7022447.1	0.0	97.68	Abscisic acid 8'-hydroxylase 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038887333.1	2.13e-304	85.68	abscisic acid 8'-hydroxylase 4 [Benincasa hispida]	[more]

BLAST of Cp4.1LG04g08030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHU3	0.0	98.11	abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC11143448...	[more]
A0A6J1JMR7	0.0	97.68	abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486079 ...	[more]
A0A1S3BZQ3	7.98e-297	84.38	abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1	[more]
A0A6J1I8M8	7.07e-295	84.73	abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 ...	[more]
A0A6J1C2D9	1.32e-294	84.66	abscisic acid 8'-hydroxylase 4 OS=Momordica charantia OX=3673 GN=LOC111007825 PE...	[more]

BLAST of Cp4.1LG04g08030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19270.1	5.7e-187	66.88	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT5G45340.1	6.2e-157	58.50	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	3.1e-156	57.21	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT2G29090.1	2.5e-150	56.62	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	2.5e-150	56.62	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 339..350 score: 33.62 coord: 410..419 score: 47.79 coord: 419..430 score: 39.37 coord: 282..299 score: 35.42
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 38..445 e-value: 4.7E-71 score: 239.9
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 419..442 score: 30.11 coord: 409..419 score: 47.18 coord: 66..85 score: 27.21 coord: 338..356 score: 22.97 coord: 271..288 score: 21.19 coord: 378..402 score: 28.63
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..469 e-value: 2.3E-117 score: 394.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..468
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 11..469
None	No IPR available	PANTHER	PTHR24286:SF103	CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASE	coord: 11..469
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 412..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g08030.1	Cp4.1LG04g08030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0010295	(+)-abscisic acid 8'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen