Cp4.1LG04g00530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g00530
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheavy metal-associated isoprenylated plant protein 36-like isoform X1
LocationCp4.1LG04: 1573469 .. 1575407 (+)
RNA-Seq ExpressionCp4.1LG04g00530
SyntenyCp4.1LG04g00530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACATTTGCCAGCCCAAAGAACATGTTTTTCTTAAAAAAAACAAAAAGACTTTCCTTTTTCTTTTTCTCTTTCTTTTTCTTTTGTTTATTATAACCTTGTAGCCCATAACTGTTGATCAATACTTCTCTCTCTGCTCCATTCTTGAGCTGCTAATGGCAAAACCAGAAGCTAAAGACTTCGAAGCGACCTCAGAACCCTTCAACTCTAAGGTAAAATGTTCTTGAATTGTTCTTCATTTTCAAATCTTTCTTATTTTCTGCTAAAATTTTGCAGACATGTTTGTTGCGAGTCTCCATTCACTGTGAAGGTTGCAAGAGGAAAGTGATCAAGATTCTCCATAATATTCATGGTACTGTTCTTCATTTTTTCTTCATTTCTTTACATTTTGATCGCTTTCTTTTACTCAAATCTTAATTTCCTCTGTTTTTCCTTCATTTATTTTGTTAAAAATTCAAAATTCCTCTCGTTTCCTCATCTGGGTATGCCTTATTTTTCCTCTAACATGAATTTCTTTCATCAAATTCTTCATTTCTTCACATTTCTTGCCCTTCCTCAAAATTTGTTCATTGTTTTCTCTTAAAAAATCGAAAATTTCCATTGTTGTTCATCTGGGTGTTCTTCAATCTCCACAATTACAGGAGTTCACGGAGTAGAAATCGATCGAAAGCAGCAGAAAGTCACAGTAACAACGAATATAGACGAACAAACATTGATTAAAAGGTTAATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAAGAATACCCATCTATAAAAACATCAAAGAAAAGCAAATCGCTATGGAAATCCCTGTCTTTGTAACCAGCGCCGCCGTGCACGACGGCGGAATGCAGAAACAGAGCACGGAAACAGAGGCTCCGGCGAAAGAAGTGCCAGTCCCATCAAAAAATGAGGAAAACTGTGGAATTAGTGGAGCTGCCCAGGGCAACGATGGAGACGGAGCTGCGGCGAAGATTGGCCGCGCCACCGTGGAAAGAGTTGTGGAGCATACATCGGGAGGAGGTGGTGATAAGAATATCGCCGGTGAGACTCAACCTGGTGGGGCAAGTGGCGGTGCGGTAGCTATCGAGGCAAGCAGCGGTGGCGGCGGAGAAGGGAGGAAGAAGAAGAGGAAAGGGCAGAAGAAAGAGAAGAGTGGCGGAGGAGGCGGCGGCGCTGTTGTCGGAGATGTGGAGGTTCCACAGGTAGTTCCTCCACCTGGCAGCATAGGATCAGCCACTCCTCATGATCATCACCATCATCATGATCCAACTCCATCATCCAATCACGCCCCTCCATTTAACCAACCATACTACCATCCAGTACATACGATCTCATCCCAACCGACTCACGTAGCGAGTTACAATACGATCTCACCTCAACCGACTCACATAGCAAGTTATAACACGATCTCATCCCAACCGACTCACATGGCAAGTTATAACACAATCTCATCCCAACCAACTCACGTGACGAGTTACAATACGACACACCCAACTACCACCTACGGTCCTTACTATGTCGAGCCACTGCCATATTCATATGTACATTCAGTTGCAGTAAGAAACGCTTCACCACCATCACCACCACCACAACCACAACCATTGACAGAACTATCAAACTCTCCGACGTCTAATCCATTCGACTTCTTTAGCGAGGAGAATCCCAGTGGTTGCACGGTGATGTGAGAATAAAAATTGTGCTTGTTTGGACAATTTTGGACATCGAAAAGACATGAAACTTGCACAAGTTGTATGAGTATTTGGGGGTAGAATTGTAATATAGATGTATATGGAGGGATAAAGTATGAAAAGAACAAAAGAAAAGGGAAAAAAAAAGAACAACTTTGTGGGTTATTGTGCTTAGCTTTATTTAGTGTACTTCTTTTAAAGCTGCACTTTAGTCTTTATCTTTTCTTTAGATTAAATTATAAATA

mRNA sequence

TACATTTGCCAGCCCAAAGAACATGTTTTTCTTAAAAAAAACAAAAAGACTTTCCTTTTTCTTTTTCTCTTTCTTTTTCTTTTGTTTATTATAACCTTGTAGCCCATAACTGTTGATCAATACTTCTCTCTCTGCTCCATTCTTGAGCTGCTAATGGCAAAACCAGAAGCTAAAGACTTCGAAGCGACCTCAGAACCCTTCAACTCTAAGACATGTTTGTTGCGAGTCTCCATTCACTGTGAAGGTTGCAAGAGGAAAGTGATCAAGATTCTCCATAATATTCATGGAGTTCACGGAGTAGAAATCGATCGAAAGCAGCAGAAAGTCACAGTAACAACGAATATAGACGAACAAACATTGATTAAAAGGTTAATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAAGAATACCCATCTATAAAAACATCAAAGAAAAGCAAATCGCTATGGAAATCCCTGTCTTTGTAACCAGCGCCGCCGTGCACGACGGCGGAATGCAGAAACAGAGCACGGAAACAGAGGCTCCGGCGAAAGAAGTGCCAGTCCCATCAAAAAATGAGGAAAACTGTGGAATTAGTGGAGCTGCCCAGGGCAACGATGGAGACGGAGCTGCGGCGAAGATTGGCCGCGCCACCGTGGAAAGAGTTGTGGAGCATACATCGGGAGGAGGTGGTGATAAGAATATCGCCGGTGAGACTCAACCTGGTGGGGCAAGTGGCGGTGCGGTAGCTATCGAGGCAAGCAGCGGTGGCGGCGGAGAAGGGAGGAAGAAGAAGAGGAAAGGGCAGAAGAAAGAGAAGAGTGGCGGAGGAGGCGGCGGCGCTGTTGTCGGAGATGTGGAGGTTCCACAGGTAGTTCCTCCACCTGGCAGCATAGGATCAGCCACTCCTCATGATCATCACCATCATCATGATCCAACTCCATCATCCAATCACGCCCCTCCATTTAACCAACCATACTACCATCCAGTACATACGATCTCATCCCAACCGACTCACGTAGCGAGTTACAATACGATCTCACCTCAACCGACTCACATAGCAAGTTATAACACGATCTCATCCCAACCGACTCACATGGCAAGTTATAACACAATCTCATCCCAACCAACTCACGTGACGAGTTACAATACGACACACCCAACTACCACCTACGGTCCTTACTATGTCGAGCCACTGCCATATTCATATGTACATTCAGTTGCAGTAAGAAACGCTTCACCACCATCACCACCACCACAACCACAACCATTGACAGAACTATCAAACTCTCCGACGTCTAATCCATTCGACTTCTTTAGCGAGGAGAATCCCAGTGGTTGCACGGTGATGTGAGAATAAAAATTGTGCTTGTTTGGACAATTTTGGACATCGAAAAGACATGAAACTTGCACAAGTTGTATGAGTATTTGGGGGTAGAATTGTAATATAGATGTATATGGAGGGATAAAGTATGAAAAGAACAAAAGAAAAGGGAAAAAAAAAGAACAACTTTGTGGGTTATTGTGCTTAGCTTTATTTAGTGTACTTCTTTTAAAGCTGCACTTTAGTCTTTATCTTTTCTTTAGATTAAATTATAAATA

Coding sequence (CDS)

ATGGCAAAACCAGAAGCTAAAGACTTCGAAGCGACCTCAGAACCCTTCAACTCTAAGACATGTTTGTTGCGAGTCTCCATTCACTGTGAAGGTTGCAAGAGGAAAGTGATCAAGATTCTCCATAATATTCATGGAGTTCACGGAGTAGAAATCGATCGAAAGCAGCAGAAAGTCACAGTAACAACGAATATAGACGAACAAACATTGATTAAAAGGTTAATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAAGAATACCCATCTATAAAAACATCAAAGAAAAGCAAATCGCTATGGAAATCCCTGTCTTTGTAACCAGCGCCGCCGTGCACGACGGCGGAATGCAGAAACAGAGCACGGAAACAGAGGCTCCGGCGAAAGAAGTGCCAGTCCCATCAAAAAATGAGGAAAACTGTGGAATTAGTGGAGCTGCCCAGGGCAACGATGGAGACGGAGCTGCGGCGAAGATTGGCCGCGCCACCGTGGAAAGAGTTGTGGAGCATACATCGGGAGGAGGTGGTGATAAGAATATCGCCGGTGAGACTCAACCTGGTGGGGCAAGTGGCGGTGCGGTAGCTATCGAGGCAAGCAGCGGTGGCGGCGGAGAAGGGAGGAAGAAGAAGAGGAAAGGGCAGAAGAAAGAGAAGAGTGGCGGAGGAGGCGGCGGCGCTGTTGTCGGAGATGTGGAGGTTCCACAGGTAGTTCCTCCACCTGGCAGCATAGGATCAGCCACTCCTCATGATCATCACCATCATCATGATCCAACTCCATCATCCAATCACGCCCCTCCATTTAACCAACCATACTACCATCCAGTACATACGATCTCATCCCAACCGACTCACGTAGCGAGTTACAATACGATCTCACCTCAACCGACTCACATAGCAAGTTATAACACGATCTCATCCCAACCGACTCACATGGCAAGTTATAACACAATCTCATCCCAACCAACTCACGTGACGAGTTACAATACGACACACCCAACTACCACCTACGGTCCTTACTATGTCGAGCCACTGCCATATTCATATGTACATTCAGTTGCAGTAAGAAACGCTTCACCACCATCACCACCACCACAACCACAACCATTGACAGAACTATCAAACTCTCCGACGTCTAATCCATTCGACTTCTTTAGCGAGGAGAATCCCAGTGGTTGCACGGTGATGTGA

Protein sequence

MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQSTETEAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVRNASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM
Homology
BLAST of Cp4.1LG04g00530 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.4e-21
Identity = 131/400 (32.75%), Postives = 168/400 (42.00%), Query Frame = 0

Query: 9   FEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQT 68
           FE   EP    T +LRVSIHCEGCKRK+ KIL  I GV+   ID KQQKVTV  N++ + 
Sbjct: 20  FEDYPEPLRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEI 79

Query: 69  LIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQSTETEAPAK 128
           LIK+++KAG+HAE WP  +    NI                  +D   QK+      P K
Sbjct: 80  LIKKIMKAGRHAELWPTSME--NNIN-----------------NDCNYQKK------PKK 139

Query: 129 EVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIAGETQPGGA 188
           +      NEE  G     + N+ +     +G            GGGG  N     Q    
Sbjct: 140 D------NEETSGDEDDDENNNNNNGGGDVG----------GGGGGGGGNFDQVKQVVTF 199

Query: 189 SGGAVAIEASSGGGGEGRKKKRKGQKKE-------KSGGGGGGAVVGDVEVPQVVPPPGS 248
             G +      G G   +KKK+K +KK          GGGGGG   G    PQ   PP +
Sbjct: 200 VNGQL---QPQGDGAPKKKKKKKKKKKSLGNTTVVMEGGGGGGGGGGP---PQNDGPPET 259

Query: 249 IGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASYN 308
           +  + P DHH  H P P  +H    +Q   HP  T+ S P H        PQ      Y 
Sbjct: 260 VIYSAPQDHHIIHGPPPHLHH----HQQQNHPYPTVHSPPRH-------HPQ----QMYG 319

Query: 309 TISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYG-----PYYVEP--LPYSYVHSVAVR 368
                P     +    + P++  SYNT H     G      YY  P   P SY +S    
Sbjct: 320 PGPGLPPPSFYHTQPQTAPSYTVSYNTVHGPINNGGNETASYYTAPPSHPTSY-YSYEYV 352

Query: 369 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 395
           +    SPPP+         S  S  F+  SE NP+ C VM
Sbjct: 380 DTGYESPPPEFYSY----RSQPSESFESLSEGNPNSCCVM 352

BLAST of Cp4.1LG04g00530 vs. ExPASy Swiss-Prot
Match: Q9C7J6 (Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX=3702 GN=HIPP35 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.9e-18
Identity = 125/401 (31.17%), Postives = 170/401 (42.39%), Query Frame = 0

Query: 15  PFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLI 74
           P   K+C L+VSIHCEGCKRKV KIL +I GV  V+ID KQ KVTV   I  + L+K+L 
Sbjct: 36  PLPYKSCTLKVSIHCEGCKRKVKKILTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLN 95

Query: 75  KAGKHAEPWPE--------RIPIYKNIKEKQIAMEIPVFVTSAAVH---DGGMQKQSTET 134
           KAGK+AE  PE          P+    K K+   E  V +T+ A     D   +  S E 
Sbjct: 96  KAGKNAEQLPEIPDPVDNKPKPVDPKEKNKKKKKEEKVQITNEATSSGIDNPEKPGSGEC 155

Query: 135 EAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIAGET 194
           + P  E PV    +E C +SG     DG   +  +     + + E  SG          +
Sbjct: 156 DKPESEKPV----DEKC-LSG-----DGGETSGPVKEEKKDVLKEKDSG-----KEESPS 215

Query: 195 QPGGASGGAVAIEASSGGGG------EGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVP 254
            P  +S  A   +A   GG        G+KKK+KGQ    +     G         Q +P
Sbjct: 216 PPADSSAPAAEKKAEDTGGAVPDNGKVGKKKKKKGQSLATTNNPTDGPA-----RTQSLP 275

Query: 255 PPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHI 314
           PP    +AT +D               P NQ   H + T ++ P H      + P P   
Sbjct: 276 PP----TATDYDR--------------PINQINDHHITTTNNPPRH-----DLYPYP--- 335

Query: 315 ASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTY--GPYYVEPLPYSYVHSVAVRN 374
                         +    + Q  +  SYN   P  +     YY  P PYSY +   + N
Sbjct: 336 --------------AQGYYAPQVMYGVSYNVAQPPVSVDAASYYTPPPPYSYAY---MHN 364

Query: 375 ASPPSP--PPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 395
              PS   P QP+P         S+ F+ FS+ENP+GC +M
Sbjct: 396 GYQPSDQNPCQPRP---------SDSFELFSDENPNGCAIM 364

BLAST of Cp4.1LG04g00530 vs. ExPASy Swiss-Prot
Match: F4JZL7 (Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX=3702 GN=HIPP33 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-14
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V+IHC+GCK+KV KIL  I GV   +ID +  KVTV+ N+D   LIK+L+K+GK
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 79 HAEPW 84
          HAE W
Sbjct: 70 HAEIW 74

BLAST of Cp4.1LG04g00530 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.1e-14
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V+IHC+GCK+KV KIL  I GV   +ID +Q KVTV+ ++D   LIK+L K+GK
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGK 69

Query: 79 HAEPW 84
          HAE W
Sbjct: 70 HAEIW 74

BLAST of Cp4.1LG04g00530 vs. ExPASy Swiss-Prot
Match: Q0WV37 (Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX=3702 GN=HIPP34 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-13
Identity = 36/63 (57.14%), Postives = 48/63 (76.19%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V++HCEGCK KV K L  I GV+ V+ D +Q +VTVT NID   L+K+L K+GK
Sbjct: 10 QTCVLKVNVHCEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGNIDPALLVKKLSKSGK 69

Query: 79 HAE 82
          HAE
Sbjct: 70 HAE 72

BLAST of Cp4.1LG04g00530 vs. NCBI nr
Match: XP_023530257.1 (heavy metal-associated isoprenylated plant protein 36-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 772 bits (1994), Expect = 2.40e-281
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIA 180
           TETEAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIA
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIA 180

Query: 181 GETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVPPPG 240
           GETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVPPPG
Sbjct: 181 GETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVPPPG 240

Query: 241 SIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASY 300
           SIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASY
Sbjct: 241 SIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASY 300

Query: 301 NTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVRNASPPS 360
           NTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVRNASPPS
Sbjct: 301 NTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVRNASPPS 360

Query: 361 PPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           PPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM
Sbjct: 361 PPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394

BLAST of Cp4.1LG04g00530 vs. NCBI nr
Match: XP_022934017.1 (heavy metal-associated isoprenylated plant protein 36-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 715 bits (1846), Expect = 1.04e-258
Identity = 373/400 (93.25%), Postives = 380/400 (95.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGGGGD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGGGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGG--GAVVGDVEVPQ 240
           KNIAGETQPGGA G AVA+EASSGGGGEGRKK RKGQKKEKSGGGGG  GA VGDVE PQ
Sbjct: 181 KNIAGETQPGGAGGSAVAVEASSGGGGEGRKK-RKGQKKEKSGGGGGSGGAAVGDVECPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGS+GSATPHDHHH HDPTPSSNHAPPFNQPYYHPVHTISSQPTH+ASYNTISPQP
Sbjct: 241 VVPPPGSLGSATPHDHHHQHDPTPSSNHAPPFNQPYYHPVHTISSQPTHIASYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
           TH+ASYNTISSQ TH+ASYNTISSQPTH+TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR
Sbjct: 301 THMASYNTISSQQTHVASYNTISSQPTHMTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N S PSPP  PQPLTELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSLPSPPSPPQPLTELSNSPTSNPFDFFSDENPSGCTVM 399

BLAST of Cp4.1LG04g00530 vs. NCBI nr
Match: KAG6588483.1 (Heavy metal-associated isoprenylated plant protein 36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706 bits (1822), Expect = 4.71e-255
Identity = 373/403 (92.56%), Postives = 379/403 (94.04%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGGGGD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGGGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGGG---AVVGDVEVP 240
           KNIAGETQPGGA G AVA+EASSGGGGEGRKKKRKGQKKEKSGGGGGG   AVVGDVE P
Sbjct: 181 KNIAGETQPGGAGGSAVAVEASSGGGGEGRKKKRKGQKKEKSGGGGGGGGGAVVGDVEGP 240

Query: 241 QVVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQ 300
           QVVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYY     ISSQPTH+ASYNTISPQ
Sbjct: 241 QVVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYY----PISSQPTHIASYNTISPQ 300

Query: 301 PTHIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAV 360
           PTH+ASYN ISSQ TH+ASYNTISSQPTH+TSYNTTHPTTTYGPYYVEPLPYSYVHSVAV
Sbjct: 301 PTHMASYNMISSQQTHVASYNTISSQPTHMTSYNTTHPTTTYGPYYVEPLPYSYVHSVAV 360

Query: 361 RNASPPSPP--PQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           RN S PSPP  PQPQPLTELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 RNTSLPSPPSPPQPQPLTELSNSPTSNPFDFFSDENPSGCTVM 399

BLAST of Cp4.1LG04g00530 vs. NCBI nr
Match: XP_022934018.1 (heavy metal-associated isoprenylated plant protein 36-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 689 bits (1777), Expect = 2.08e-248
Identity = 363/400 (90.75%), Postives = 368/400 (92.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGGGGD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGGGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGG--GAVVGDVEVPQ 240
           KNIAGETQPGGA G AVA+EASSGGGGEGRKK RKGQKKEKSGGGGG  GA VGDVE PQ
Sbjct: 181 KNIAGETQPGGAGGSAVAVEASSGGGGEGRKK-RKGQKKEKSGGGGGSGGAAVGDVECPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGS+GSATPHDHHH HDPTPSSNHAPPFNQPYYHPVHTISSQPTH+ASYNTISPQP
Sbjct: 241 VVPPPGSLGSATPHDHHHQHDPTPSSNHAPPFNQPYYHPVHTISSQPTHIASYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
           TH+ASYNTISSQPTHM             TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR
Sbjct: 301 THMASYNTISSQPTHM-------------TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N S PSPP  PQPLTELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSLPSPPSPPQPLTELSNSPTSNPFDFFSDENPSGCTVM 386

BLAST of Cp4.1LG04g00530 vs. NCBI nr
Match: XP_022969682.1 (heavy metal-associated isoprenylated plant protein 36-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 673 bits (1736), Expect = 4.93e-242
Identity = 358/400 (89.50%), Postives = 364/400 (91.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFE TSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVH VEIDR QQKVTV
Sbjct: 1   MAKPEAKDFEGTSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHAVEIDRNQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQ AMEIPV V SAAVHDGG QKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQNAMEIPVSVNSAAVHDGGKQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGG GD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGSGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKS--GGGGGGAVVGDVEVPQ 240
           KNIAGETQPGGA GGA A+ ASSGGGGEGRKKKRKGQKKEKS  GGGGGGAVVGD+E PQ
Sbjct: 181 KNIAGETQPGGAGGGAAAV-ASSGGGGEGRKKKRKGQKKEKSDGGGGGGGAVVGDMEGPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPY H VHTISSQPTHV SYNTISPQP
Sbjct: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYNHSVHTISSQPTHVTSYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
            HI SYNT+SSQPTH+ASYNTISSQPTH+ SYNTTHPTTTYG YYVEPLPYS    V VR
Sbjct: 301 IHITSYNTVSSQPTHVASYNTISSQPTHMASYNTTHPTTTYGSYYVEPLPYS----VVVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N SPPSPP QPQPL ELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSPPSPP-QPQPLAELSNSPTSNPFDFFSDENPSGCTVM 394

BLAST of Cp4.1LG04g00530 vs. ExPASy TrEMBL
Match: A0A6J1F1H2 (heavy metal-associated isoprenylated plant protein 36-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441249 PE=4 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 5.03e-259
Identity = 373/400 (93.25%), Postives = 380/400 (95.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGGGGD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGGGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGG--GAVVGDVEVPQ 240
           KNIAGETQPGGA G AVA+EASSGGGGEGRKK RKGQKKEKSGGGGG  GA VGDVE PQ
Sbjct: 181 KNIAGETQPGGAGGSAVAVEASSGGGGEGRKK-RKGQKKEKSGGGGGSGGAAVGDVECPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGS+GSATPHDHHH HDPTPSSNHAPPFNQPYYHPVHTISSQPTH+ASYNTISPQP
Sbjct: 241 VVPPPGSLGSATPHDHHHQHDPTPSSNHAPPFNQPYYHPVHTISSQPTHIASYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
           TH+ASYNTISSQ TH+ASYNTISSQPTH+TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR
Sbjct: 301 THMASYNTISSQQTHVASYNTISSQPTHMTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N S PSPP  PQPLTELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSLPSPPSPPQPLTELSNSPTSNPFDFFSDENPSGCTVM 399

BLAST of Cp4.1LG04g00530 vs. ExPASy TrEMBL
Match: A0A6J1F6H1 (heavy metal-associated isoprenylated plant protein 36-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441249 PE=4 SV=1)

HSP 1 Score: 689 bits (1777), Expect = 1.01e-248
Identity = 363/400 (90.75%), Postives = 368/400 (92.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV
Sbjct: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQIAMEIPVFVTSAAVHDGGMQKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGGGGD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGGGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKSGGGGG--GAVVGDVEVPQ 240
           KNIAGETQPGGA G AVA+EASSGGGGEGRKK RKGQKKEKSGGGGG  GA VGDVE PQ
Sbjct: 181 KNIAGETQPGGAGGSAVAVEASSGGGGEGRKK-RKGQKKEKSGGGGGSGGAAVGDVECPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGS+GSATPHDHHH HDPTPSSNHAPPFNQPYYHPVHTISSQPTH+ASYNTISPQP
Sbjct: 241 VVPPPGSLGSATPHDHHHQHDPTPSSNHAPPFNQPYYHPVHTISSQPTHIASYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
           TH+ASYNTISSQPTHM             TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR
Sbjct: 301 THMASYNTISSQPTHM-------------TSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N S PSPP  PQPLTELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSLPSPPSPPQPLTELSNSPTSNPFDFFSDENPSGCTVM 386

BLAST of Cp4.1LG04g00530 vs. ExPASy TrEMBL
Match: A0A6J1HX13 (heavy metal-associated isoprenylated plant protein 36-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468631 PE=4 SV=1)

HSP 1 Score: 673 bits (1736), Expect = 2.39e-242
Identity = 358/400 (89.50%), Postives = 364/400 (91.00%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFE TSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVH VEIDR QQKVTV
Sbjct: 1   MAKPEAKDFEGTSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHAVEIDRNQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQ AMEIPV V SAAVHDGG QKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQNAMEIPVSVNSAAVHDGGKQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGG GD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGSGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKS--GGGGGGAVVGDVEVPQ 240
           KNIAGETQPGGA GGA A+ ASSGGGGEGRKKKRKGQKKEKS  GGGGGGAVVGD+E PQ
Sbjct: 181 KNIAGETQPGGAGGGAAAV-ASSGGGGEGRKKKRKGQKKEKSDGGGGGGGAVVGDMEGPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPY H VHTISSQPTHV SYNTISPQP
Sbjct: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYNHSVHTISSQPTHVTSYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
            HI SYNT+SSQPTH+ASYNTISSQPTH+ SYNTTHPTTTYG YYVEPLPYS    V VR
Sbjct: 301 IHITSYNTVSSQPTHVASYNTISSQPTHMASYNTTHPTTTYGSYYVEPLPYS----VVVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N SPPSPP QPQPL ELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSPPSPP-QPQPLAELSNSPTSNPFDFFSDENPSGCTVM 394

BLAST of Cp4.1LG04g00530 vs. ExPASy TrEMBL
Match: A0A6J1HX26 (heavy metal-associated isoprenylated plant protein 36-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111468631 PE=4 SV=1)

HSP 1 Score: 649 bits (1674), Expect = 4.09e-233
Identity = 350/400 (87.50%), Postives = 354/400 (88.50%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFE TSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVH VEIDR QQKVTV
Sbjct: 1   MAKPEAKDFEGTSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHAVEIDRNQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQ AMEIPV V SAAVHDGG QKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQNAMEIPVSVNSAAVHDGGKQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGG GD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGSGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKS--GGGGGGAVVGDVEVPQ 240
           KNIAGETQPGGA GGA A+ ASSGGGGEGRKKKRKGQKKEKS  GGGGGGAVVGD+E PQ
Sbjct: 181 KNIAGETQPGGAGGGAAAV-ASSGGGGEGRKKKRKGQKKEKSDGGGGGGGAVVGDMEGPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPY H VHTISSQPTHV SYNTISPQP
Sbjct: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYNHSVHTISSQPTHVTSYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
           TH+ASYNTISSQPTHMASY             NTTHPTTTYG YYVEPLPYS    V VR
Sbjct: 301 THVASYNTISSQPTHMASY-------------NTTHPTTTYGSYYVEPLPYS----VVVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N SPPSPP QPQPL ELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSPPSPP-QPQPLAELSNSPTSNPFDFFSDENPSGCTVM 381

BLAST of Cp4.1LG04g00530 vs. ExPASy TrEMBL
Match: A0A6J1I3D2 (heavy metal-associated isoprenylated plant protein 36-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468631 PE=4 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 6.76e-232
Identity = 349/400 (87.25%), Postives = 353/400 (88.25%), Query Frame = 0

Query: 1   MAKPEAKDFEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTV 60
           MAKPEAKDFE TSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVH VEIDR QQKVTV
Sbjct: 1   MAKPEAKDFEGTSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHAVEIDRNQQKVTV 60

Query: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQS 120
           TTNIDEQTLIKRLIKAGKHAEPWPERIPI KNIKEKQ AMEIPV V SAAVHDGG QKQS
Sbjct: 61  TTNIDEQTLIKRLIKAGKHAEPWPERIPISKNIKEKQNAMEIPVSVNSAAVHDGGKQKQS 120

Query: 121 TETEAPAKEVPVPSKNEENCGISGAAQ----GNDGDGAAAKIGRATVERVVEHTSGGGGD 180
           TETEAPAKEVPVPSKNEENCGISGAAQ     NDGDGAAAKIGRATVERVVE TSGG GD
Sbjct: 121 TETEAPAKEVPVPSKNEENCGISGAAQILEISNDGDGAAAKIGRATVERVVEFTSGGSGD 180

Query: 181 KNIAGETQPGGASGGAVAIEASSGGGGEGRKKKRKGQKKEKS--GGGGGGAVVGDVEVPQ 240
           KNIAGETQPGGA GGA A+ ASSGGGGEGRKKKRKGQKKEKS  GGGGGGAVVGD+E PQ
Sbjct: 181 KNIAGETQPGGAGGGAAAV-ASSGGGGEGRKKKRKGQKKEKSDGGGGGGGAVVGDMEGPQ 240

Query: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQP 300
           VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPY H VHTISSQPTHV SYNTISPQP
Sbjct: 241 VVPPPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYNHSVHTISSQPTHVTSYNTISPQP 300

Query: 301 THIASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYGPYYVEPLPYSYVHSVAVR 360
            H+ASYNTISSQPTHMASY             NTTHPTTTYG YYVEPLPYS    V VR
Sbjct: 301 IHMASYNTISSQPTHMASY-------------NTTHPTTTYGSYYVEPLPYS----VVVR 360

Query: 361 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 394
           N SPPSPP QPQPL ELSNSPTSNPFDFFS+ENPSGCTVM
Sbjct: 361 NTSPPSPP-QPQPLAELSNSPTSNPFDFFSDENPSGCTVM 381

BLAST of Cp4.1LG04g00530 vs. TAIR 10
Match: AT5G27690.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 9.7e-23
Identity = 131/400 (32.75%), Postives = 168/400 (42.00%), Query Frame = 0

Query: 9   FEATSEPFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQT 68
           FE   EP    T +LRVSIHCEGCKRK+ KIL  I GV+   ID KQQKVTV  N++ + 
Sbjct: 20  FEDYPEPLRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEI 79

Query: 69  LIKRLIKAGKHAEPWPERIPIYKNIKEKQIAMEIPVFVTSAAVHDGGMQKQSTETEAPAK 128
           LIK+++KAG+HAE WP  +    NI                  +D   QK+      P K
Sbjct: 80  LIKKIMKAGRHAELWPTSME--NNIN-----------------NDCNYQKK------PKK 139

Query: 129 EVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIAGETQPGGA 188
           +      NEE  G     + N+ +     +G            GGGG  N     Q    
Sbjct: 140 D------NEETSGDEDDDENNNNNNGGGDVG----------GGGGGGGGNFDQVKQVVTF 199

Query: 189 SGGAVAIEASSGGGGEGRKKKRKGQKKE-------KSGGGGGGAVVGDVEVPQVVPPPGS 248
             G +      G G   +KKK+K +KK          GGGGGG   G    PQ   PP +
Sbjct: 200 VNGQL---QPQGDGAPKKKKKKKKKKKSLGNTTVVMEGGGGGGGGGGP---PQNDGPPET 259

Query: 249 IGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHIASYN 308
           +  + P DHH  H P P  +H    +Q   HP  T+ S P H        PQ      Y 
Sbjct: 260 VIYSAPQDHHIIHGPPPHLHH----HQQQNHPYPTVHSPPRH-------HPQ----QMYG 319

Query: 309 TISSQPTHMASYNTISSQPTHVTSYNTTHPTTTYG-----PYYVEP--LPYSYVHSVAVR 368
                P     +    + P++  SYNT H     G      YY  P   P SY +S    
Sbjct: 320 PGPGLPPPSFYHTQPQTAPSYTVSYNTVHGPINNGGNETASYYTAPPSHPTSY-YSYEYV 352

Query: 369 NASPPSPPPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 395
           +    SPPP+         S  S  F+  SE NP+ C VM
Sbjct: 380 DTGYESPPPEFYSY----RSQPSESFESLSEGNPNSCCVM 352

BLAST of Cp4.1LG04g00530 vs. TAIR 10
Match: AT1G56210.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.3e-19
Identity = 125/401 (31.17%), Postives = 170/401 (42.39%), Query Frame = 0

Query: 15  PFNSKTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLI 74
           P   K+C L+VSIHCEGCKRKV KIL +I GV  V+ID KQ KVTV   I  + L+K+L 
Sbjct: 36  PLPYKSCTLKVSIHCEGCKRKVKKILTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLN 95

Query: 75  KAGKHAEPWPE--------RIPIYKNIKEKQIAMEIPVFVTSAAVH---DGGMQKQSTET 134
           KAGK+AE  PE          P+    K K+   E  V +T+ A     D   +  S E 
Sbjct: 96  KAGKNAEQLPEIPDPVDNKPKPVDPKEKNKKKKKEEKVQITNEATSSGIDNPEKPGSGEC 155

Query: 135 EAPAKEVPVPSKNEENCGISGAAQGNDGDGAAAKIGRATVERVVEHTSGGGGDKNIAGET 194
           + P  E PV    +E C +SG     DG   +  +     + + E  SG          +
Sbjct: 156 DKPESEKPV----DEKC-LSG-----DGGETSGPVKEEKKDVLKEKDSG-----KEESPS 215

Query: 195 QPGGASGGAVAIEASSGGGG------EGRKKKRKGQKKEKSGGGGGGAVVGDVEVPQVVP 254
            P  +S  A   +A   GG        G+KKK+KGQ    +     G         Q +P
Sbjct: 216 PPADSSAPAAEKKAEDTGGAVPDNGKVGKKKKKKGQSLATTNNPTDGPA-----RTQSLP 275

Query: 255 PPGSIGSATPHDHHHHHDPTPSSNHAPPFNQPYYHPVHTISSQPTHVASYNTISPQPTHI 314
           PP    +AT +D               P NQ   H + T ++ P H      + P P   
Sbjct: 276 PP----TATDYDR--------------PINQINDHHITTTNNPPRH-----DLYPYP--- 335

Query: 315 ASYNTISSQPTHMASYNTISSQPTHVTSYNTTHPTTTY--GPYYVEPLPYSYVHSVAVRN 374
                         +    + Q  +  SYN   P  +     YY  P PYSY +   + N
Sbjct: 336 --------------AQGYYAPQVMYGVSYNVAQPPVSVDAASYYTPPPPYSYAY---MHN 364

Query: 375 ASPPSP--PPQPQPLTELSNSPTSNPFDFFSEENPSGCTVM 395
              PS   P QP+P         S+ F+ FS+ENP+GC +M
Sbjct: 396 GYQPSDQNPCQPRP---------SDSFELFSDENPNGCAIM 364

BLAST of Cp4.1LG04g00530 vs. TAIR 10
Match: AT5G19090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-15
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V+IHC+GCK+KV KIL  I GV   +ID +  KVTV+ N+D   LIK+L+K+GK
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 79 HAEPW 84
          HAE W
Sbjct: 70 HAEIW 74

BLAST of Cp4.1LG04g00530 vs. TAIR 10
Match: AT5G19090.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-15
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V+IHC+GCK+KV KIL  I GV   +ID +  KVTV+ N+D   LIK+L+K+GK
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 79 HAEPW 84
          HAE W
Sbjct: 70 HAEIW 74

BLAST of Cp4.1LG04g00530 vs. TAIR 10
Match: AT5G19090.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-15
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 19 KTCLLRVSIHCEGCKRKVIKILHNIHGVHGVEIDRKQQKVTVTTNIDEQTLIKRLIKAGK 78
          +TC+L+V+IHC+GCK+KV KIL  I GV   +ID +  KVTV+ N+D   LIK+L+K+GK
Sbjct: 10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 79 HAEPW 84
          HAE W
Sbjct: 70 HAEIW 74

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84J881.4e-2132.75Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
Q9C7J61.9e-1831.17Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX... [more]
F4JZL71.6e-1456.92Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX... [more]
Q9M8K52.1e-1456.92Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX... [more]
Q0WV372.3e-1357.14Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_023530257.12.40e-281100.00heavy metal-associated isoprenylated plant protein 36-like [Cucurbita pepo subsp... [more]
XP_022934017.11.04e-25893.25heavy metal-associated isoprenylated plant protein 36-like isoform X1 [Cucurbita... [more]
KAG6588483.14.71e-25592.56Heavy metal-associated isoprenylated plant protein 36, partial [Cucurbita argyro... [more]
XP_022934018.12.08e-24890.75heavy metal-associated isoprenylated plant protein 36-like isoform X2 [Cucurbita... [more]
XP_022969682.14.93e-24289.50heavy metal-associated isoprenylated plant protein 36-like isoform X1 [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1F1H25.03e-25993.25heavy metal-associated isoprenylated plant protein 36-like isoform X1 OS=Cucurbi... [more]
A0A6J1F6H11.01e-24890.75heavy metal-associated isoprenylated plant protein 36-like isoform X2 OS=Cucurbi... [more]
A0A6J1HX132.39e-24289.50heavy metal-associated isoprenylated plant protein 36-like isoform X1 OS=Cucurbi... [more]
A0A6J1HX264.09e-23387.50heavy metal-associated isoprenylated plant protein 36-like isoform X3 OS=Cucurbi... [more]
A0A6J1I3D26.76e-23287.25heavy metal-associated isoprenylated plant protein 36-like isoform X2 OS=Cucurbi... [more]
Match NameE-valueIdentityDescription
AT5G27690.19.7e-2332.75Heavy metal transport/detoxification superfamily protein [more]
AT1G56210.11.3e-1931.17Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.11.2e-1556.92Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.21.2e-1556.92Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.31.2e-1556.92Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 24..78
e-value: 3.9E-11
score: 43.2
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 19..82
score: 10.417914
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 23..81
e-value: 1.35412E-9
score: 51.8377
NoneNo IPR availableGENE3D3.30.70.100coord: 17..80
e-value: 1.4E-16
score: 62.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 167..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 370..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..154
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 312..394
coord: 1..318
NoneNo IPR availablePANTHERPTHR45868:SF46HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 35coord: 312..394
coord: 1..318
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 18..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g00530.1Cp4.1LG04g00530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding