Cp4.1LG03g11960 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g11960
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG03: 10777213 .. 10782033 (+)
RNA-Seq Expression	Cp4.1LG03g11960
Synteny	Cp4.1LG03g11960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTCCAAAATTGGAGAATCCTTTCCAACCAACAATTTCCCACTTTATTTCTCAAACCTTCCGTATTAAATCTCATTCCTCAATCTTCCAAATTCCAATTCATTTCGTTTCATTCCTCAAGAATCGTTCTCTTCATCTCCAAAATCATGGCTGATTCATCCTCGAATTCCCTATGCAATCTGATTCACATTGCATTCGCAATCGCTTTTGCTCTGTTAATCTCATCTCCGATCGGTTCTTACGGCTTTACTGCGCAAAATGTGATCAATTCGCCATTGTTGACGAAGAAGATCGCCACAAATCGCACGATCAAGGTTGATATCAACGGAAATGGCGAGTTCACGTCCATTCAAGCTGCAGTCGATGCTGTTCCGGAGGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTACAGGTGATTTCTAGCGTTTTCTCTTCCGGATTCTTACATTGATGTGATAAATTGTTCGTTTGTGTTTTTAATTGACGAAAGAGTGCGTGTTCTTGGCTCTGTAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGAAACGGAAAAGGAAGGACGCACATGGTTTGGTCACAGAGCTCTAATGATAACATTGAATCTGCTACTTTTAAAGTCGAAGCCAAACATTTCATTGCTTTTGGAATTAGCTTCAAGGTCCAACATATTTCTCGATTCCTTTGGTGATTTTTGCCTAAATTAGCTTCAAAATGTATTCAGTTATTCCATTGATTTCTCTCTTTTTTCGTTTATTTCTTCTGGAAATTTTCCTATACAGAATGAGGCTCCGACTGGAGTGGCTTACACATCGCAAAATCAGTCGGTAGCGGCATTCATTGCCGCAGATAAAGTCGCATTTTACCATTGTGGATTCTACAGCACTCACAATACGCTGTTCGATTACAAAGGCAGACATTATTATGACGGTTGCTACATTCAAGGCTCAATTGATTTTATCTTTGGCCGCGGCAAATCCGTCTTCCACGTAATTCTTTTCCCTTCCTTTCTCTACTGTTGCTTGCTCTCTGAAGAATTTAGAATACCTTTGGTGTTTTATACATGGAATATTTACAGTTCTCTTAATAACCGTTTCATATTTCATTTTTGGATTCTGGTTTTTAGTTTCTTAAAACTAAATTTATAAACGTTATTCTACCTCTTTCCATGTTTGTTCTTCACTTTCTAGTAATGTTTTCAAAACTACTTACAAATAGTTTTCAAACATAAACGAGGATGGTTGGGAGGAGTCCTACATTGGTTAGTTTAGGTAATGATCATAGGTTTTAAGCAAGGAATACATCTCCATTGATATGAGGTCTTTTGGGAAGCCTAAAGCAAAGTCATGAGAACTTATGCTCAAAGTGGATAATATCATACCATTGTGGGGATCAGTGTTTCCTAACATGGTATATGAATCATGCCTTTAACTTAGCCATGTTAATAGAATCCTCAAATGTCGAACAAAGAAGTTGTGAGCCTTGAAGTCAAAAGTGACTCAAGTGTCGAATAAATAGTGTACTTTGTTTGAGGACTCCATACTTTGTTTGAGGACTCCATACTTTGTTTGAGGACTCCATACTTTGTTTGAGGACTCCATACTTTGTTTGAGGACTCTAGAAAAGGAGTCGAGCCTCGATTAAGGGGAGACAGTTCGATGGCTCCATCAGGGGAGGCTCTATAGTATAGTTTATTCGAGGGGAGGATTGTAGGGGATCATAGGTTTATAAGTGAGGAATACATCTCCATTGGTATACATACGAGTTTATGCTCAAAGTGAACAATATCATGCAACTGTAAAGATAGGTAATGATAGGTAATTCGTAACAGCATGCATATTTGATTTGAAAAAAATGATGTAGTGTGTTAAAAACAGGTCAACTTAAGAATTTAGAAGCTCATGTAACCAAGTAATTTCATTATAGTCTAATCTTTCTTTCATTTTTCTTGGTGGGATGTGATGATTTTCCATGAAAAACGATGTTATATTGTTGCTGTGGATGCAGAATTGTGAGATGTTTGTGATTGATGACAAGAGATTGACGGTCCGTGGTTCAGTCACAGCTCAAAACAGAAAAAGTGCAGATGAGAACAGCGGGTTTGTTTTCATCAGAGGAAAGGTTTACGGAATTGGCGGTGTGTATTTGGGGAGAGCAAAAGGTGCATTTTCCAGAGTCGTCTTTGCCAAGACTTACTTCTCCATGACTGTTCTTCCTGCTGGTTGGACCAATTGGAGCTATTCTGGTGGCACAGAGTAAGCACTCCCTCTCTCCCCCATTTACGCGTTTATAATTGCTAGGAATCATGCTAGGAATCACGTACCATGCTGCGATATTGTCTACTTTGAGCATAAGTTCTCATACCAATAGAGATATATTCCTTACTTATAAACCTACTATCATCCTCTAAATTGGCCAATGGGAGACTCCCTCTCAACAATTCTCAACATGAACTAGTCTTTAACTAGTTGAATTATGTTTGATTTAGTCGTTTACTGAATTATTAGGAATCACGAACCTCCATAATTGTAGGATACTTTCTACTTTGAACATAAGCTCTCATGACTTTATTTCTTTGTTTTCCCACACCCCTCGTGCCAATTGAGATATATTCCTTATTTATAAATCCACTATCATCCTCTAAATTTGCCAATGTGAGACTCCCTCTCAACAATTCTCAACATGAACTAGTCTTTAACTAGTTGAATTATACTTGATTTAGCTGTTTACTGAACTATTAGGAATTACGAACCTCTATAATAGTATGATACTTTCCAATTTGAACATAAGCTCTCTGACTTTGTTTTTTGTTTTTCCAAACAACCTCATGCCAATTGAGATATATTCCTTATTTTTAAATTCATGATCATCCTCTAAATTAGTTAGCGTAAAATTTCCTCCCAACAATCTTCAACTTGAACTAGTTTTTAATGGTTGATAATTACTTGAACTAAACTAGTATTTTTGTAAGTTCAACCAGTGTTTTTGTGATTACAGGAATCTGTATCATTCAGAATATGATTGCCATGGACCAGGAGCAGAAAAAGGGAACCGAGCACCATGGGCCAAACAGCTAACTGACAAGGAAGCTGACCAGTATATGGACATCAGCTTTATTGAAGGGGCTGATTGGCTTCCTGCGTGGCTATAAGCAAAAAACAGAAAAAAAAAAAAAAAAAAAAAGGCTAAGCTAATTCACTCCTCTCGCAAGTCCTTCTCCCCTTTATTTTTATAAATTTACGGTCTATTCGGGTTCTTTTTGCAAGCTATTTCGCAAAGATTTCGCAAAGATTCTTAAAACTATTTGTTCCAATGAGACGAAATATTGAATTCAAATTCTTCTCTTTTTTTTTTTTATTTTTTTTTTTAAATCTTTTTCTGGTTATCTTATTACGTAGAATTGGAAGTAGAAATAGGTAGTGGTCTTTGCATGAGCATTTTCTGGTGGCTGCATGCATATTAGCTTTTGTAAATATATTGGGAAAATTTCCAGGACTTTGGGAGTATCTATATCTACTCCCTTTTTTAACATCATATTTGATTTGGTCTGATTTTAGACTCTGCCACCGAATCCTCCCCCTGAACCGCCACCAAAGCCTCCTCCTCCACCTGCTCCGCCTCCAAATCCACCTCCTTTACCACCTCCAAAACCTCCTCCTGCTCCGCCCCCATGGCCTCCTCCGAAACCACCTCCTGCTCCCCCGCCAGCTCCGCCTCCAAAGCCCCCTCCTGCTCCTCCACCAGCTCCGCCTCCAAATCCACCTCCCGCTCCGCCTCCAAATCCACCTCCCGCTCCGCCTCCATGGCCACCTCCGAAACCGCCTCCTGCTCCACCACCAGCTCCACCTCCTGCTCCGCCTCCAAACCCACCTCCTGCTCCGCCTCCTGCTCCGCCTCCCGCTCCGCCTCCGAAACCACCTCCTTTACCACCTCCGAAACCACCTCCCGCTCCCCCACCAGCTCCGCCTCCAAAACCACCGCCTGCTCCGCCACCAGCTCCACCTCCAGCTCCACCTCCAAATCCACCTCCTTTACCACCTCCAAATCCACCTCCAGCTCCGCCTCCTAATCCACCTCCCCCTCCAATTCCGCCGCCATGGCCACCTCCGAATCCACCTCCAGCTCCGCCTCCTAATCCACCGCCGCCTCCTAATCCACCTCCACCTCCTAATCCACCGCCATGCCCACCTCCAAAACCACCACCAAGGCCACCTCCAGCTCCGGCTCCAGCTCCACCTCCAAACCCACCACCAGCACCACCGCCAAGACCACCACCAAGCGTCTCCTTCTCCAACTTCCGGCTTTCAGCAAATCCCACAATCAAAAATGCCAGAACAAGAGCCAAGCCTCCAACCTTGTAAGTAATTTTGGCCATACTCCTTAAGCTAGAAAGAGTAGAGCTCACTTTTTGAATGCACAATAATGCATTGTAGAGATTATTGTTATTATTTATAGCGAGAGAAGTGCAGGCAGGGGAACAGCATTAATGCATGCAGTTGAGACTCCTCTTGTTTGACTGTTTTAGTTGGTTGGGACACTCTTTAGAACTTAATGAAATGCCTAAGTTTTCAACTAAGGGGCCGTTGAGTTCCTTAATTTGCTTGGCTCCACCTCTCATCTGCTAACCGATTTTACAGCACAGATCCATCCATTTTCCTGTATTTGCACAAAAAGTCCTCCTCCAATGCCCCACTTGGTCCCATCTGAGCCACGGACTCTCAGTTTTCTTTGAATTATTCATAGACCCAATTATGCATGGGTTAGATTTGTGGGAAGTCATCATTCCAATACACA

mRNA sequence

CCCTCCAAAATTGGAGAATCCTTTCCAACCAACAATTTCCCACTTTATTTCTCAAACCTTCCGTATTAAATCTCATTCCTCAATCTTCCAAATTCCAATTCATTTCGTTTCATTCCTCAAGAATCGTTCTCTTCATCTCCAAAATCATGGCTGATTCATCCTCGAATTCCCTATGCAATCTGATTCACATTGCATTCGCAATCGCTTTTGCTCTGTTAATCTCATCTCCGATCGGTTCTTACGGCTTTACTGCGCAAAATGTGATCAATTCGCCATTGTTGACGAAGAAGATCGCCACAAATCGCACGATCAAGGTTGATATCAACGGAAATGGCGAGTTCACGTCCATTCAAGCTGCAGTCGATGCTGTTCCGGAGGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTACAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGAAACGGAAAAGGAAGGACGCACATGGTTTGGTCACAGAGCTCTAATGATAACATTGAATCTGCTACTTTTAAAGTCGAAGCCAAACATTTCATTGCTTTTGGAATTAGCTTCAAGAATGAGGCTCCGACTGGAGTGGCTTACACATCGCAAAATCAGTCGGTAGCGGCATTCATTGCCGCAGATAAAGTCGCATTTTACCATTGTGGATTCTACAGCACTCACAATACGCTGTTCGATTACAAAGGCAGACATTATTATGACGGTTGCTACATTCAAGGCTCAATTGATTTTATCTTTGGCCGCGGCAAATCCGTCTTCCACAATTGTGAGATGTTTGTGATTGATGACAAGAGATTGACGGTCCGTGGTTCAGTCACAGCTCAAAACAGAAAAAGTGCAGATGAGAACAGCGGGAATCTGTATCATTCAGAATATGATTGCCATGGACCAGGAGCAGAAAAAGGGAACCGAGCACCATGGGCCAAACAGCTAACTGACAAGGAAGCTGACCAGTATATGGACATCAGCTTTATTGAAGGGGCTGATTGGCTTCCTGCGTGGCTATAAGCAAAAAACAGAAAAAAAAAAAAAAAAAAAAAGGCTAAGCTAATTCACTCCTCTCGCAAGTCCTTCTCCCCTTTATTTTTATAAATTTACGGTCTATTCGGGTTCTTTTTGCAAGCTATTTCGCAAAGATTTCGCAAAGATTCTTAAAACTATTTGTTCCAATGAGACGAAATATTGAATTCAAATTCTTCTCTTTTTTTTTTTTATTTTTTTTTTTAAATCTTTTTCTGGTTATCTTATTACGTAGAATTGGAAGTAGAAATAGGTAGTGGTCTTTGCATGAGCATTTTCTGGTGGCTGCATGCATATTAGCTTTTGTAAATATATTGGGAAAATTTCCAGGACTTTGGGAGTATCTATATCTACTCCCTTTTTTAACATCATATTTGATTTGGTCTGATTTTAGACTCTGCCACCGAATCCTCCCCCTGAACCGCCACCAAAGCCTCCTCCTCCACCTGCTCCGCCTCCAAATCCACCTCCTTTACCACCTCCAAAACCTCCTCCTGCTCCGCCCCCATGGCCTCCTCCGAAACCACCTCCTGCTCCCCCGCCAGCTCCGCCTCCAAAGCCCCCTCCTGCTCCTCCACCAGCTCCGCCTCCAAATCCACCTCCCGCTCCGCCTCCAAATCCACCTCCCGCTCCGCCTCCATGGCCACCTCCGAAACCGCCTCCTGCTCCACCACCAGCTCCACCTCCTGCTCCGCCTCCAAACCCACCTCCTGCTCCGCCTCCTGCTCCGCCTCCCGCTCCGCCTCCGAAACCACCTCCTTTACCACCTCCGAAACCACCTCCCGCTCCCCCACCAGCTCCGCCTCCAAAACCACCGCCTGCTCCGCCACCAGCTCCACCTCCAGCTCCACCTCCAAATCCACCTCCTTTACCACCTCCAAATCCACCTCCAGCTCCGCCTCCTAATCCACCTCCCCCTCCAATTCCGCCGCCATGGCCACCTCCGAATCCACCTCCAGCTCCGCCTCCTAATCCACCGCCGCCTCCTAATCCACCTCCACCTCCTAATCCACCGCCATGCCCACCTCCAAAACCACCACCAAGGCCACCTCCAGCTCCGGCTCCAGCTCCACCTCCAAACCCACCACCAGCACCACCGCCAAGACCACCACCAAGCGTCTCCTTCTCCAACTTCCGGCTTTCAGCAAATCCCACAATCAAAAATGCCAGAACAAGAGCCAAGCCTCCAACCTTGTAAGTAATTTTGGCCATACTCCTTAAGCTAGAAAGAGTAGAGCTCACTTTTTGAATGCACAATAATGCATTGTAGAGATTATTGTTATTATTTATAGCGAGAGAAGTGCAGGCAGGGGAACAGCATTAATGCATGCAGTTGAGACTCCTCTTGTTTGACTGTTTTAGTTGGTTGGGACACTCTTTAGAACTTAATGAAATGCCTAAGTTTTCAACTAAGGGGCCGTTGAGTTCCTTAATTTGCTTGGCTCCACCTCTCATCTGCTAACCGATTTTACAGCACAGATCCATCCATTTTCCTGTATTTGCACAAAAAGTCCTCCTCCAATGCCCCACTTGGTCCCATCTGAGCCACGGACTCTCAGTTTTCTTTGAATTATTCATAGACCCAATTATGCATGGGTTAGATTTGTGGGAAGTCATCATTCCAATACACA

Coding sequence (CDS)

ATGGCTGATTCATCCTCGAATTCCCTATGCAATCTGATTCACATTGCATTCGCAATCGCTTTTGCTCTGTTAATCTCATCTCCGATCGGTTCTTACGGCTTTACTGCGCAAAATGTGATCAATTCGCCATTGTTGACGAAGAAGATCGCCACAAATCGCACGATCAAGGTTGATATCAACGGAAATGGCGAGTTCACGTCCATTCAAGCTGCAGTCGATGCTGTTCCGGAGGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTACAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGAAACGGAAAAGGAAGGACGCACATGGTTTGGTCACAGAGCTCTAATGATAACATTGAATCTGCTACTTTTAAAGTCGAAGCCAAACATTTCATTGCTTTTGGAATTAGCTTCAAGAATGAGGCTCCGACTGGAGTGGCTTACACATCGCAAAATCAGTCGGTAGCGGCATTCATTGCCGCAGATAAAGTCGCATTTTACCATTGTGGATTCTACAGCACTCACAATACGCTGTTCGATTACAAAGGCAGACATTATTATGACGGTTGCTACATTCAAGGCTCAATTGATTTTATCTTTGGCCGCGGCAAATCCGTCTTCCACAATTGTGAGATGTTTGTGATTGATGACAAGAGATTGACGGTCCGTGGTTCAGTCACAGCTCAAAACAGAAAAAGTGCAGATGAGAACAGCGGGAATCTGTATCATTCAGAATATGATTGCCATGGACCAGGAGCAGAAAAAGGGAACCGAGCACCATGGGCCAAACAGCTAACTGACAAGGAAGCTGACCAGTATATGGACATCAGCTTTATTGAAGGGGCTGATTGGCTTCCTGCGTGGCTATAA

Protein sequence

MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDINGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWSQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRKSADENSGNLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQYMDISFIEGADWLPAWL

Homology

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BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot
Match: Q9LSP1 (Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.7e-99
Identity = 179/332 (53.92%), Postives = 223/332 (67.17%), Query Frame = 0


Query: 19  IAFALLISSPIGSYGFTAQNV-INSPLLTKKIATNRTIKVDINGNGEFTSIQAAVDAVPE 78
           +   L++ S  GS     Q    ++PLLT+KIATNR+I VDI G G++TS+Q A+DAVP 
Sbjct: 9   LVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVPV 68

Query: 79  GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWSQSSNDNIESATFKVEAK 138
           GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T +  SQSS DN+ SATFKVEA 
Sbjct: 69  GNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEAN 128

Query: 139 HFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDG 198
           HF+AFGIS +N+AP G+A+TS+NQSVAAF+AADKVAFYHC FYS HNTLFD KGRHYY  
Sbjct: 129 HFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYHE 188

Query: 199 CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRKSADENSG---------- 258
           CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GS+TA +R+SA+E +G          
Sbjct: 189 CYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVYG 248

Query: 259 ------------------------------------------NLYHSEYDCHGPGAEKGN 298
                                                     NLYH EY CHGPGAE+  
Sbjct: 249 IDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQK 308

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.5e-46
Identity = 110/305 (36.07%), Postives = 161/305 (52.79%), Query Frame = 0


Query: 45  LTKKIATNRTIKVDINGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPY 104
           L  +  T+  + VD++G G F+++Q+A+D VP+ +S   +I V  G YREKV +  +K  
Sbjct: 81  LRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTN 140

Query: 105 IFLRGNGKGRTHMVW---SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQ 164
           + ++G G   T + W   ++S+ +  +S +F V A +F A+ ISFKN AP      +  Q
Sbjct: 141 LVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQ 200

Query: 165 SVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMF 224
           +VA  I  D+ AFY CGFY   +TL D KGRH++  C+IQGSIDFIFG G+S++ +C + 
Sbjct: 201 AVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTIN 260

Query: 225 VIDDKRLT-VRGSVTAQNRKSADENSG-----------------------------NLYH 284
            I     + V GS+TAQ R+S DE SG                             N Y 
Sbjct: 261 SIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYM 320

Query: 285 S-----------------------EYDCHGPGAEKGNRAPWAKQLTDKEADQYMDISFIE 294
           S                       E+ C+GPGA+   R  + KQLTD EA  ++D+SFI+
Sbjct: 321 SGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFID 380

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 112/343 (32.65%), Postives = 161/343 (46.94%), Query Frame = 0


Query: 5   SSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDINGNGE 64
           S  S+ N  HI   I   + I S I S    + +  +S       +T   I+VD +G G+
Sbjct: 8   SKRSIANYHHI---IIINIFILSSITSSSMASSSSPSS----IDFSTAILIRVDQSGKGD 67

Query: 65  FTSIQAAVDAVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWSQS 124
           F+ IQ A++++P    NSQ   I V+ GIYREKV IP+ KPYI L G     T ++WS  
Sbjct: 68  FSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWS-D 127

Query: 125 SNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFYST 184
             D +ES T  + A  F+   ++ +N+      + +  ++VA  +AADK AFY C   S 
Sbjct: 128 GEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKAAFYGCVITSY 187

Query: 185 HNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRKSA 244
            +TL D  G HY+  CYI+G+ DFI G   S++  C +  +        GS+TAQ R SA
Sbjct: 188 QDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NGSITAQMRTSA 247

Query: 245 DENSG----------------------------------------------------NLY 294
            E SG                                                     +Y
Sbjct: 248 TEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKENTVY 307

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 104/302 (34.44%), Postives = 147/302 (48.68%), Query Frame = 0


Query: 51  TNRTIKVDINGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 110
           T   + VD NG   FT++Q+AVDAV   + +  +I +  G+Y EKV IP +KP I L+G 
Sbjct: 87  TTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQ 146

Query: 111 GKGRTHMVWSQ---SSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFI 170
           G   T + W+    S+N     AT +V    F+A  ISF N AP         Q+VA  I
Sbjct: 147 GFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRI 206

Query: 171 AADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDK- 230
           A D+ AF  CGF+   +TL D +GRHY+  CYIQGSIDFIFG  KS++ +C +  + ++ 
Sbjct: 207 AGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQL 266

Query: 231 ---RLTVRGSVTAQNRKSADENSG------------------------------------ 290
                 V G+VTA  R S DENSG                                    
Sbjct: 267 SPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDV 326

Query: 291 ----------------NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQYMDISFIEGAD 294
                            +++ EY+C GPGA+   RAP+ ++L + +    ++ SFI+G  
Sbjct: 327 IAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQ 386

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.4e-40
Identity = 96/308 (31.17%), Postives = 149/308 (48.38%), Query Frame = 0


Query: 48  KIATNRTIKVDINGN-GEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 107
           K+  + T+ V    N G+FT IQ A+D++P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 108 LRGNGKGRTHMVWSQSSN---------DNIESATFKVEAKHFIAFGISFKNEAPTGVAYT 167
           + G G  +T + W  ++              SA+F V +  F+A  I+F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 168 SQNQSVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHN 227
              Q+VA  ++AD  AF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 228 CEMFVIDDKRLTVRGSVTAQNRKSADENSG------------------------------ 287
           C +  I DK     G+VTAQ R S  E++G                              
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 288 ----------------------NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQYMDIS 294
                                  +++ +Y C G GA  G R  WA++LTD+EA  ++ ++
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

[Close]

BLAST of Cp4.1LG03g11960 vs. NCBI nr
Match: XP_023526591.1 (probable pectinesterase 67 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582 bits (1500), Expect = 1.72e-208
Identity = 297/349 (85.10%), Postives = 297/349 (85.10%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. NCBI nr
Match: KAG7018566.1 (putative pectinesterase 67 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 579 bits (1492), Expect = 2.84e-207
Identity = 294/349 (84.24%), Postives = 297/349 (85.10%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRTHMVWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. NCBI nr
Match: KAG6582165.1 (putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 576 bits (1485), Expect = 3.31e-206
Identity = 293/349 (83.95%), Postives = 296/349 (84.81%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLCN IHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRTHMVWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. NCBI nr
Match: XP_022955586.1 (probable pectinesterase 67 [Cucurbita moschata])

HSP 1 Score: 576 bits (1484), Expect = 4.70e-206
Identity = 293/349 (83.95%), Postives = 296/349 (84.81%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLCN IHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRTHMVWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRL+VRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLSVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. NCBI nr
Match: XP_022979848.1 (probable pectinesterase 67 [Cucurbita maxima])

HSP 1 Score: 565 bits (1455), Expect = 1.23e-201
Identity = 286/349 (81.95%), Postives = 293/349 (83.95%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLC  IHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRTH+VWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAF+AAD VAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLTVRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL
Match: A0A6J1GU67 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 2.28e-206
Identity = 293/349 (83.95%), Postives = 296/349 (84.81%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLCN IHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRTHMVWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRL+VRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLSVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL
Match: A0A6J1IXH1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1)

HSP 1 Score: 565 bits (1455), Expect = 5.97e-202
Identity = 286/349 (81.95%), Postives = 293/349 (83.95%), Query Frame = 0


Query: 1   MADSSSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60
           MADSSSNSLC  IHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN
Sbjct: 1   MADSSSNSLCKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDIN 60

Query: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWS 120
           GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRTH+VWS
Sbjct: 61  GNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVWS 120

Query: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFY 180
           QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAF+AAD VAFYHCGFY
Sbjct: 121 QSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGFY 180

Query: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRK 240
           STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLTVRGSVTAQNRK
Sbjct: 181 STHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNRK 240

Query: 241 SADENSG----------------------------------------------------N 297
           SADENSG                                                    N
Sbjct: 241 SADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTEN 300

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL
Match: A0A0A0LAR0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 9.18e-181
Identity = 259/350 (74.00%), Postives = 280/350 (80.00%), Query Frame = 0


Query: 1   MADSSSNSL-CNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60
           MADSSSNSL    I+IAFAIAF  LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180
           SQSS+DN+ESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAF+AADK+AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSADENSG---------------------------------------------------- 297
           KSA+ENSG                                                    
Sbjct: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL
Match: A0A5A7U7L5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001570 PE=4 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 1.07e-179
Identity = 260/350 (74.29%), Postives = 277/350 (79.14%), Query Frame = 0


Query: 1   MADSSSNSL-CNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60
           MADSSSNSL    I+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAF+AADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSADENSG---------------------------------------------------- 297
           KSA+ENSG                                                    
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

[Close]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL
Match: A0A1S3AY88 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 1.07e-179
Identity = 260/350 (74.29%), Postives = 277/350 (79.14%), Query Frame = 0


Query: 1   MADSSSNSL-CNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60
           MADSSSNSL    I+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAF+AADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSADENSG---------------------------------------------------- 297
           KSA+ENSG                                                    
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

[Close]

BLAST of Cp4.1LG03g11960 vs. TAIR 10
Match: AT3G17060.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.3e-100
Identity = 179/332 (53.92%), Postives = 223/332 (67.17%), Query Frame = 0


Query: 19  IAFALLISSPIGSYGFTAQNV-INSPLLTKKIATNRTIKVDINGNGEFTSIQAAVDAVPE 78
           +   L++ S  GS     Q    ++PLLT+KIATNR+I VDI G G++TS+Q A+DAVP 
Sbjct: 9   LVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVPV 68

Query: 79  GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWSQSSNDNIESATFKVEAK 138
           GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T +  SQSS DN+ SATFKVEA 
Sbjct: 69  GNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEAN 128

Query: 139 HFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDG 198
           HF+AFGIS +N+AP G+A+TS+NQSVAAF+AADKVAFYHC FYS HNTLFD KGRHYY  
Sbjct: 129 HFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYHE 188

Query: 199 CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRKSADENSG---------- 258
           CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GS+TA +R+SA+E +G          
Sbjct: 189 CYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVYG 248

Query: 259 ------------------------------------------NLYHSEYDCHGPGAEKGN 298
                                                     NLYH EY CHGPGAE+  
Sbjct: 249 IDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQK 308

[Close]

BLAST of Cp4.1LG03g11960 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.5e-47
Identity = 110/305 (36.07%), Postives = 161/305 (52.79%), Query Frame = 0


Query: 45  LTKKIATNRTIKVDINGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPY 104
           L  +  T+  + VD++G G F+++Q+A+D VP+ +S   +I V  G YREKV +  +K  
Sbjct: 81  LRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTN 140

Query: 105 IFLRGNGKGRTHMVW---SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQ 164
           + ++G G   T + W   ++S+ +  +S +F V A +F A+ ISFKN AP      +  Q
Sbjct: 141 LVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQ 200

Query: 165 SVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMF 224
           +VA  I  D+ AFY CGFY   +TL D KGRH++  C+IQGSIDFIFG G+S++ +C + 
Sbjct: 201 AVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTIN 260

Query: 225 VIDDKRLT-VRGSVTAQNRKSADENSG-----------------------------NLYH 284
            I     + V GS+TAQ R+S DE SG                             N Y 
Sbjct: 261 SIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYM 320

Query: 285 S-----------------------EYDCHGPGAEKGNRAPWAKQLTDKEADQYMDISFIE 294
           S                       E+ C+GPGA+   R  + KQLTD EA  ++D+SFI+
Sbjct: 321 SGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFID 380

[Close]

BLAST of Cp4.1LG03g11960 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 9.2e-42
Identity = 104/302 (34.44%), Postives = 147/302 (48.68%), Query Frame = 0


Query: 51  TNRTIKVDINGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 110
           T   + VD NG   FT++Q+AVDAV   + +  +I +  G+Y EKV IP +KP I L+G 
Sbjct: 87  TTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQ 146

Query: 111 GKGRTHMVWSQ---SSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFI 170
           G   T + W+    S+N     AT +V    F+A  ISF N AP         Q+VA  I
Sbjct: 147 GFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRI 206

Query: 171 AADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDK- 230
           A D+ AF  CGF+   +TL D +GRHY+  CYIQGSIDFIFG  KS++ +C +  + ++ 
Sbjct: 207 AGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQL 266

Query: 231 ---RLTVRGSVTAQNRKSADENSG------------------------------------ 290
                 V G+VTA  R S DENSG                                    
Sbjct: 267 SPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDV 326

Query: 291 ----------------NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQYMDISFIEGAD 294
                            +++ EY+C GPGA+   RAP+ ++L + +    ++ SFI+G  
Sbjct: 327 IAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQ 386

[Close]

BLAST of Cp4.1LG03g11960 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 168.3 bits (425), Expect = 9.2e-42
Identity = 112/343 (32.65%), Postives = 161/343 (46.94%), Query Frame = 0


Query: 5   SSNSLCNLIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDINGNGE 64
           S  S+ N  HI   I   + I S I S    + +  +S       +T   I+VD +G G+
Sbjct: 8   SKRSIANYHHI---IIINIFILSSITSSSMASSSSPSS----IDFSTAILIRVDQSGKGD 67

Query: 65  FTSIQAAVDAVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTHMVWSQS 124
           F+ IQ A++++P    NSQ   I V+ GIYREKV IP+ KPYI L G     T ++WS  
Sbjct: 68  FSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWS-D 127

Query: 125 SNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGFYST 184
             D +ES T  + A  F+   ++ +N+      + +  ++VA  +AADK AFY C   S 
Sbjct: 128 GEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKAAFYGCVITSY 187

Query: 185 HNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNRKSA 244
            +TL D  G HY+  CYI+G+ DFI G   S++  C +  +        GS+TAQ R SA
Sbjct: 188 QDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NGSITAQMRTSA 247

Query: 245 DENSG----------------------------------------------------NLY 294
            E SG                                                     +Y
Sbjct: 248 TEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKENTVY 307

[Close]

BLAST of Cp4.1LG03g11960 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 4.6e-41
Identity = 96/308 (31.17%), Postives = 149/308 (48.38%), Query Frame = 0


Query: 48  KIATNRTIKVDINGN-GEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 107
           K+  + T+ V    N G+FT IQ A+D++P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 108 LRGNGKGRTHMVWSQSSN---------DNIESATFKVEAKHFIAFGISFKNEAPTGVAYT 167
           + G G  +T + W  ++              SA+F V +  F+A  I+F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 168 SQNQSVAAFIAADKVAFYHCGFYSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHN 227
              Q+VA  ++AD  AF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 228 CEMFVIDDKRLTVRGSVTAQNRKSADENSG------------------------------ 287
           C +  I DK     G+VTAQ R S  E++G                              
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 288 ----------------------NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQYMDIS 294
                                  +++ +Y C G GA  G R  WA++LTD+EA  ++ ++
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g11960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSP1	4.7e-99	53.92	Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1	[more]
Q9ZQA3	3.5e-46	36.07	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q9SIJ9	1.3e-40	32.65	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
O23038	1.3e-40	34.44	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q8VYZ3	6.4e-40	31.17	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]

BLAST of Cp4.1LG03g11960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526591.1	1.72e-208	85.10	probable pectinesterase 67 [Cucurbita pepo subsp. pepo]	[more]
KAG7018566.1	2.84e-207	84.24	putative pectinesterase 67 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6582165.1	3.31e-206	83.95	putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022955586.1	4.70e-206	83.95	probable pectinesterase 67 [Cucurbita moschata]	[more]
XP_022979848.1	1.23e-201	81.95	probable pectinesterase 67 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG03g11960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GU67	2.28e-206	83.95	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1	[more]
A0A6J1IXH1	5.97e-202	81.95	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1	[more]
A0A0A0LAR0	9.18e-181	74.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1	[more]
A0A5A7U7L5	1.07e-179	74.29	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00157...	[more]
A0A1S3AY88	1.07e-179	74.29	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1	[more]

BLAST of Cp4.1LG03g11960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17060.1	3.3e-100	53.92	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	2.5e-47	36.07	Pectin lyase-like superfamily protein	[more]
AT1G05310.1	9.2e-42	34.44	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	9.2e-42	32.65	pectinesterase 11	[more]
AT5G19730.1	4.6e-41	31.17	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 56..247 e-value: 2.0E-42 score: 145.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 48..247 e-value: 7.6E-63 score: 214.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 248..296 e-value: 1.6E-7 score: 33.0
None	No IPR available	PANTHER	PTHR31321:SF98	PECTINESTERASE 67-RELATED	coord: 248..296
None	No IPR available	PANTHER	PTHR31321:SF98	PECTINESTERASE 67-RELATED	coord: 11..249
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 11..249 coord: 248..296
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 200..209
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 50..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g11960.1	Cp4.1LG03g11960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity