Cp4.1LG03g05050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g05050
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionlong chain acyl-CoA synthetase 1-like
LocationCp4.1LG03: 5056134 .. 5061071 (-)
RNA-Seq ExpressionCp4.1LG03g05050
SyntenyCp4.1LG03g05050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGTATGGAAGAAAATTGGGGTGATATTAAATTACTTCGTCTATAGAGCTTTAAACCAATCAATCCATGACATGTGTGTGTGCTTTCACATCCCATTGGCCATTATATTGAACTCAATTCACCATTGCTCCACCACCTCACCTCACCTCACCTTGCTTGCATTGCATGATTTTTCCTTCCTGTTTCTGCTCATACCTCTGTAAGCCACCACTTCCCTTTCCCTCTCTGTTTCTTGCTCTGTTTTTGTGTTTGTTTTGTGCATTTTGGAAGTGGGGTTTTGATACTCAACATGTTACCTCCATTTCCATGGCTGTTGAAGAACAACCTCCATTTTTCATTCACACTCTTCTATTTTATGCTTTTGTGGTGTGTTTGATCTTCTGTTCATATTCGTTATGCACAATGACTCTGTTCATAGTTAGCTTTGTAACCATTGCCATGGGTTTAGAGTTTCTCATCTTAAAAATATGAACTTTTGAAAGGTACCCATTGATCCTAATGAGCTTCTGTTATGGTTCTCTGTTTTTCTTTCAAGACTCCATAAATTTTGAGGCTTTTTGTTTCTCTGAGTCTTCAAGTAGAAGCATTTGGGCATGGTGGTTGGCCCCTCATTAATGTCTGCAGTTAGTAGGTGTCTTACTAGACCTATACAACGTTTTATGTAGAACGTTACCTTTGTAATTAATTTTTCTGAGAAGTCTTCTGTGTTCTTTTGTTTTTTCTCTTTTCCTTTCTATTGAAAGTTTCAATGGAAGTTGGGACATTTTTTGACTTCTGAACTTTGAGACAGAGTTTTCCTTGAGTTCCATTAATGATCAATGCAACAGGTTTTCCAGGAGTTCAAGAATGAAGAGGTTTTCGATCAAGGTCGAGGAAGGAAGGGAAGGCAAAGATGGGAAACCGTCCATTGGACCGGTTTATCGAAATGTTCTTGCGAAAGATGGCTCTCCTTCTCTGTTTGAGGATTTGAACACATCTTGGGATCTTTTCAGGTTATATACAGAGTTCATGTTCTGGCTATGTGTGAAATGAATGGTTTGATGTTATTGCAAGGCTTAGAATGTTAAGAACATGAAATTGGTTTCTTGGATTGTTTTGCAGTGTTTCAGCTGAAAAGTATGCTGGAAGGCAAATGCTCGGATGGCGAAAATTTGTCGAGGGGAAGGTGAATTGAACTCTCGTAATCTATATGAAAAGAAAACATGAAAGAAATGTATATGAACTTGAATGGTTTCGGTATCCAGCCAGGACCTTACATATGGAAAACTTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGTTCTGCACTACGCGCTGTTGGCGCCAAACCGGTAAGCATACTCCTGCAGTTTCAAGAGCATACCATAACTGAGAAACTAAAGAGATGATGATTTTGCACGACAGGGCTCGAGAATCGGGATTTATGGGGAGAACTGTCCTCAATGGATAATAGCAATGGAGGTTCAGCTGAAACCTTCTTCAATTTTGTAGTTGTTTCATTGTATCAGGTAACAAATTCTCTAAATGTTTCGTGTAGGCCTGCAATGCCCACAGTATGATCTGTGTGCCACTCTATGATACCCTTGGTATATTTCATCTCTTTGTAAGTAATAACTTCAAGTTGAACTTCCTGTTGATTCTTAAACTAATAGAGAAGAGGCTAAATGAATTGCAGGACATGAAGCTGTTAACTTCATTATAGACCATGCAGAGATCGATATCGTTTTCGTCCAAGATAAGAAAGCAAAAGAAGTAAGAATAGCTTTCTAGTTAATATAATATGCTTTCACATCTCAATCTAAACCAAAAAAGAGGCACTTACTAGACATGGTTGATATTCAATGTTCATTTTGCAGCTATTAAAGTCTGAAAGGACAAACCCTGAGAGGCTGAAAGGTGAGAATGATGAATATAAGATGGTTTTCTAGCAATCGTTGAACCAATTGACCTATTTCGATTCACTTTCGTAGTTGTTGTCTGCTTTACTTCACTCAAGGAGGAAGATAAGATCTCAGCAGCAGACAATGGGGTGAAACCATACTCATGGGACGAGTTCTTTCGCCTGGTCGGTTTTAAATCTAAATGACGTTTTAGCATCCTTTACTATACGTTGGAGTTGACGCATGAATCTACTTTGTATCAGGGAAAAGAAAATCCTTCTGAGATCACTCCTCCACGACTTCTCGACATTTGCACGATTATGTACACAAGTGGAACTAGTGGAACTCCTAAAGGTGTTGTGTTAACACATGAAACCTTAGTAACGTTTATTAGAGGAGTTGATATCTACATGGAGCAGTTTGAAGACAAGGTAGATAAGTCCCCTTATCAAACTCAGGATTATTGCTGGCTTGCATTTTCTTTTAACTTCCAAAGCTTTTTTCCTTTGTCAACAGATGACAGAGGATGATGTGTATCTATCATTCCTGCCTCTAGCTCATATCCTCGACCGAACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCTGTTGGCTACTACCATGGGGTATTGTATTTTTCATGGCATCCCCTTTTGAACCAGATTCAGATCTTTGCTTTACAAATTTTCTTTGAAGAGATGATCAGGACAGCTCATTTTTGTTTCTCATTTGTTTTATGAAGGATCTTAATGCAATAAAAGAAGATCTAATGGAGTTAAAGCCGACGCTTATAGTCGGAGTACCTCGAGTTTTTGAGAGGATTCAGGAAGGCACGAAAACACTGCTTGTTATCTGTAATTCTGTTACTATTCATAACTTGTTAACAAGGCAAAATTAAGAAATATATATTTCAGGAATCAAACAAGCTGTACAAGTACTCAATCCAGTGAGAAGAAAGGCTTTTGATTTGCTCTATAGATAGTAAGTCAACCTTGATCTTTCAGTGCCTGTGGTTTGCTTCTTTTCTACAAGATTCTTATAAGATCAAAGATGAATCATATTTATGCTGAACTTGCAGCAAACTTTCATGGATGAATAGAGGATACAAGCAGAAAGAAGCATCCCCTTTGGCAGATTTCTTGGCATTCAGGAAGGTCGAGTCCTACACTCTTAATCTATTACGTCCTCTCAATTTCTAGTTCCTTATCACTCTAAGAATCTATTTGAAATGACTTTTTTTCACCTAAAAAGCTTTTTTTGTTGTGCACTTGAAAAGTCATTTCAAACTACTTACATGTCCATGACTAACTTGATTATCCCCTAACACTAAACTTGTATGAACTCAGGTGAAAGCTAGATTAGGCGGCCGCCTTCGACTAATAATATCTGGGGGTGCAGCCTTAAGCTCAGAAGTGGAAGAGTTTCTGAGGGTCACATGTTGTGCTTTTTTTATCCAAGGATATGGTAAAGATCACTAGCTACAGAAAAACTATAGTTCAGCTTTAGTGGTAGCTTTTAGGCACATTCCTAGTTTAAAGATTCTCCATTCTTCGTTGCAATATGCTTAACGAGTTTCCTCGTCATCATTGTTGCTAATGATTTATATTGCAATGCAACATACTGTTTAAAACTTGTTCAAACATTAAGAACAGCAATGTATGGTAACATTTAGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAAATGTGTATGCTCGGAACCGTTGGGAGCGTTACTCCATTCAATGACCTTCGGTTGGAGGAGGTTCCAGAGATGGGTTACAATCCTCTTGGGGATCGTCCATGTGGTGAAATATGTGTGAGAGGAAAGTCTGTTTTCACTGAATACTACAAAAACCCTGAACTAACCAAAGAGGCAATAAAGGATGGTTGGTTTCACACAGGTGGTTCATCACTTCCCTCTCTTGTTCTTGTCTGATAATTCATTGCTATTTAACTTGTTTCTTCCATTCTTCTACTACTTCAGGAGACATAGGCGAAATCTTACCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTTTCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTCTACACGAATACCCCGATCGTCGAGGATGTAAGTTTCGAATTGTCCTTTTGAGTCTCTTTCTAAAACTAAATTACAATTTGGTGATTTGAGTTTCTTTGATATAATCTTTCCAAAGATATGGATCTATGGGAATAGCTTCAAATCAAGGCTAGTTGCAGTGGTGGTTCTACACGAGGAAAACACCAAGAAATGGGCAAATTCTAATGGCTTTTTGTGTTCTTTCTCTGAGCTTAGTTCTCTTGAACAGCTCAGAAATTATGTTCTCTCTGAGCTCACTTCAACAGCTGAAAGAAACAAGGTATTACGTTTGCTTAAACAAGAAATCTAATCCAATGGCAATTAAGTAGCTATGAAACTATAAATTTTTCTTTCTTCTCACTTAATCAAACTCAACGTCTTTGATTCACATCAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTAGAATCACACCCATTCGACATCGAAAACGATCTGGTGACAGCAACTTTGAAAAAGAAGAGAAACAATCTACTCAAACACTACGAGGTCGGTTGAAAAACTCTGTTTTTTATAGATTGAATTCTCCAAAACTGAGGTCACTTAAAGCTCCCTAATGTTGCAGGTGCAAATCAACGAAGAATATAAAAACTTGGAGGCAAAGAGGTGATGAAGTTGAAGCACAGCTGATCTGTCTGAACTAAAAATCATACATCCAAAACCATTTGGTTGTAGTAACAAAGAAACATCTCTTTCTTTCTTTTTTCTTCTTCTCGCAAATTAAGGACTATGTGGGTTCACTGACAGATGGGAGTATGGTAGTTGAATGATGCTTCAGTAGACCTCATTCTATGGAAGGGTTACAAAATAAGTTCATATATTAAAAAGCTACCATCTTCTTTGGAAAACTTTTGTTTAAGTTCCTAAAAAAATTTAAAGATCATCTTTGATCAAAA

mRNA sequence

TGTGTATGGAAGAAAATTGGGGTGATATTAAATTACTTCGTCTATAGAGCTTTAAACCAATCAATCCATGACATGTGTGTGTGCTTTCACATCCCATTGGCCATTATATTGAACTCAATTCACCATTGCTCCACCACCTCACCTCACCTCACCTTGCTTGCATTGCATGATTTTTCCTTCCTGTTTCTGCTCATACCTCTGTTTTCCAGGAGTTCAAGAATGAAGAGGTTTTCGATCAAGGTCGAGGAAGGAAGGGAAGGCAAAGATGGGAAACCGTCCATTGGACCGGTTTATCGAAATGTTCTTGCGAAAGATGGCTCTCCTTCTCTGTTTGAGGATTTGAACACATCTTGGGATCTTTTCAGTGTTTCAGCTGAAAAGTATGCTGGAAGGCAAATGCTCGGATGGCGAAAATTTGTCGAGGGGAAGCCAGGACCTTACATATGGAAAACTTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGTTCTGCACTACGCGCTGTTGGCGCCAAACCGGGCTCGAGAATCGGGATTTATGGGGAGAACTGTCCTCAATGGATAATAGCAATGGAGGCCTGCAATGCCCACAGTATGATCTGTGTGCCACTCTATGATACCCTTGGACATGAAGCTGTTAACTTCATTATAGACCATGCAGAGATCGATATCGTTTTCGTCCAAGATAAGAAAGCAAAAGAACTATTAAAGTCTGAAAGGACAAACCCTGAGAGGCTGAAAGTTGTTGTCTGCTTTACTTCACTCAAGGAGGAAGATAAGATCTCAGCAGCAGACAATGGGGTGAAACCATACTCATGGGACGAGTTCTTTCGCCTGGGAAAAGAAAATCCTTCTGAGATCACTCCTCCACGACTTCTCGACATTTGCACGATTATGTACACAAGTGGAACTAGTGGAACTCCTAAAGGTGTTGTGTTAACACATGAAACCTTAGTAACGTTTATTAGAGGAGTTGATATCTACATGGAGCAGTTTGAAGACAAGATGACAGAGGATGATGTGTATCTATCATTCCTGCCTCTAGCTCATATCCTCGACCGAACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCTGTTGGCTACTACCATGGGGATCTTAATGCAATAAAAGAAGATCTAATGGAGTTAAAGCCGACGCTTATAGTCGGAGTACCTCGAGTTTTTGAGAGGATTCAGGAAGGCACGAAAACACTGCTTGTTATCTTGAGAAGAAAGGCTTTTGATTTGCTCTATAGATACAAACTTTCATGGATGAATAGAGGATACAAGCAGAAAGAAGCATCCCCTTTGGCAGATTTCTTGGCATTCAGGAAGGTGAAAGCTAGATTAGGCGGCCGCCTTCGACTAATAATATCTGGGGGTGCAGCCTTAAGCTCAGAAGTGGAAGAGTTTCTGAGGGTCACATGTTGTGCTTTTTTTATCCAAGGATATGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAAATGTGTATGCTCGGAACCGTTGGGAGCGTTACTCCATTCAATGACCTTCGGTTGGAGGAGGTTCCAGAGATGGGTTACAATCCTCTTGGGGATCGTCCATGTGGTGAAATATGTGTGAGAGGAAAGTCTGTTTTCACTGAATACTACAAAAACCCTGAACTAACCAAAGAGGCAATAAAGGATGGTTGGTTTCACACAGGAGACATAGGCGAAATCTTACCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTTTCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTCTACACGAATACCCCGATCGTCGAGGATATATGGATCTATGGGAATAGCTTCAAATCAAGGCTAGTTGCAGTGGTGGTTCTACACGAGGAAAACACCAAGAAATGGGCAAATTCTAATGGCTTTTTGTGTTCTTTCTCTGAGCTTAGTTCTCTTGAACAGCTCAGAAATTATGTTCTCTCTGAGCTCACTTCAACAGCTGAAAGAAACAAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTAGAATCACACCCATTCGACATCGAAAACGATCTGGTGACAGCAACTTTGAAAAAGAAGAGAAACAATCTACTCAAACACTACGAGGTGCAAATCAACGAAGAATATAAAAACTTGGAGGCAAAGAGGTGATGAAGTTGAAGCACAGCTGATCTGTCTGAACTAAAAATCATACATCCAAAACCATTTGGTTGTAGTAACAAAGAAACATCTCTTTCTTTCTTTTTTCTTCTTCTCGCAAATTAAGGACTATGTGGGTTCACTGACAGATGGGAGTATGGTAGTTGAATGATGCTTCAGTAGACCTCATTCTATGGAAGGGTTACAAAATAAGTTCATATATTAAAAAGCTACCATCTTCTTTGGAAAACTTTTGTTTAAGTTCCTAAAAAAATTTAAAGATCATCTTTGATCAAAA

Coding sequence (CDS)

ATGTGTGTGTGCTTTCACATCCCATTGGCCATTATATTGAACTCAATTCACCATTGCTCCACCACCTCACCTCACCTCACCTTGCTTGCATTGCATGATTTTTCCTTCCTGTTTCTGCTCATACCTCTGTTTTCCAGGAGTTCAAGAATGAAGAGGTTTTCGATCAAGGTCGAGGAAGGAAGGGAAGGCAAAGATGGGAAACCGTCCATTGGACCGGTTTATCGAAATGTTCTTGCGAAAGATGGCTCTCCTTCTCTGTTTGAGGATTTGAACACATCTTGGGATCTTTTCAGTGTTTCAGCTGAAAAGTATGCTGGAAGGCAAATGCTCGGATGGCGAAAATTTGTCGAGGGGAAGCCAGGACCTTACATATGGAAAACTTACAAAGAAGTTTATGGTGATGTTCTTAACATTGGTTCTGCACTACGCGCTGTTGGCGCCAAACCGGGCTCGAGAATCGGGATTTATGGGGAGAACTGTCCTCAATGGATAATAGCAATGGAGGCCTGCAATGCCCACAGTATGATCTGTGTGCCACTCTATGATACCCTTGGACATGAAGCTGTTAACTTCATTATAGACCATGCAGAGATCGATATCGTTTTCGTCCAAGATAAGAAAGCAAAAGAACTATTAAAGTCTGAAAGGACAAACCCTGAGAGGCTGAAAGTTGTTGTCTGCTTTACTTCACTCAAGGAGGAAGATAAGATCTCAGCAGCAGACAATGGGGTGAAACCATACTCATGGGACGAGTTCTTTCGCCTGGGAAAAGAAAATCCTTCTGAGATCACTCCTCCACGACTTCTCGACATTTGCACGATTATGTACACAAGTGGAACTAGTGGAACTCCTAAAGGTGTTGTGTTAACACATGAAACCTTAGTAACGTTTATTAGAGGAGTTGATATCTACATGGAGCAGTTTGAAGACAAGATGACAGAGGATGATGTGTATCTATCATTCCTGCCTCTAGCTCATATCCTCGACCGAACGGTCGAGGAATATTTCTTTCGTAAAGGCGCATCTGTTGGCTACTACCATGGGGATCTTAATGCAATAAAAGAAGATCTAATGGAGTTAAAGCCGACGCTTATAGTCGGAGTACCTCGAGTTTTTGAGAGGATTCAGGAAGGCACGAAAACACTGCTTGTTATCTTGAGAAGAAAGGCTTTTGATTTGCTCTATAGATACAAACTTTCATGGATGAATAGAGGATACAAGCAGAAAGAAGCATCCCCTTTGGCAGATTTCTTGGCATTCAGGAAGGTGAAAGCTAGATTAGGCGGCCGCCTTCGACTAATAATATCTGGGGGTGCAGCCTTAAGCTCAGAAGTGGAAGAGTTTCTGAGGGTCACATGTTGTGCTTTTTTTATCCAAGGATATGGATTGACTGAAACTTGTGGACCAACAACTATTGGCTTTCCTGATGAAATGTGTATGCTCGGAACCGTTGGGAGCGTTACTCCATTCAATGACCTTCGGTTGGAGGAGGTTCCAGAGATGGGTTACAATCCTCTTGGGGATCGTCCATGTGGTGAAATATGTGTGAGAGGAAAGTCTGTTTTCACTGAATACTACAAAAACCCTGAACTAACCAAAGAGGCAATAAAGGATGGTTGGTTTCACACAGGAGACATAGGCGAAATCTTACCCAATGGGGTTGTGAAGATCATTGACAGGAAGAAGAATCTCATAAAACTTTCCCAAGGAGAATACATAGCACTCGAATACTTAGAAAATGTCTACACGAATACCCCGATCGTCGAGGATATATGGATCTATGGGAATAGCTTCAAATCAAGGCTAGTTGCAGTGGTGGTTCTACACGAGGAAAACACCAAGAAATGGGCAAATTCTAATGGCTTTTTGTGTTCTTTCTCTGAGCTTAGTTCTCTTGAACAGCTCAGAAATTATGTTCTCTCTGAGCTCACTTCAACAGCTGAAAGAAACAAGTTGAAACGTTTCGAATACATCAAAGGAGTTGTGTTAGAATCACACCCATTCGACATCGAAAACGATCTGGTGACAGCAACTTTGAAAAAGAAGAGAAACAATCTACTCAAACACTACGAGGTGCAAATCAACGAAGAATATAAAAACTTGGAGGCAAAGAGGTGA

Protein sequence

MCVCFHIPLAIILNSIHHCSTTSPHLTLLALHDFSFLFLLIPLFSRSSRMKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQMLGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEACNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTSLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLTHETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVILRRKAFDLLYRYKLSWMNRGYKQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETCGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYKNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPIVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTAERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Homology
BLAST of Cp4.1LG03g05050 vs. ExPASy Swiss-Prot
Match: O22898 (Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 5.8e-265
Identity = 423/659 (64.19%), Postives = 532/659 (80.73%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MK F+ KVEEG +G DGKPS+GPVYRN+L++ G P +  ++ T+WD+FS S EK+    M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA GA+PGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           C AH++ICVPLYDTLG  AV++I++HAEID VFVQD K K LL+ +    +RLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           ++ +E    A++ GVK YSW +F  +G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           TH+ + TF+ G+D+YM+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN +++D+ ELKPT + GVPRVFERI EG +  L  L   RR  F+ LY++KL+W+NRGY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
              +ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F +QGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
            G T +GFPDEMCMLGTVG    +N++RLEEV EMGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELT+E +KDGWFHTGDIGEILPNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           +V+DIW+YG+SFKS LVAVVV + E   +WA   GF   F EL S  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 706
           AE+NKL++FEYIK V +E+ PFD+E DLVTATLK +RNNLLK+Y+VQI+E Y+ L +K+
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Cp4.1LG03g05050 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 3.6e-198
Identity = 328/660 (49.70%), Postives = 451/660 (68.33%), Query Frame = 0

Query: 48  SRMKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGR 107
           S+ K++  +VEEG+EG DG+PS+GPVYR++ AKDG P   E +++ WD+F +S EKY   
Sbjct: 2   SQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNN 61

Query: 108 QMLGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAM 167
            MLG R+ V+GKPG Y+W+TY+EVY  V+ +G++LR+VG K  ++ GIYG N P+WII+M
Sbjct: 62  PMLGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISM 121

Query: 168 EACNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVC 227
           EACNAH + CVPLYDTLG +AV FII H+E+ IVFV++KK  EL K+   + E +K VV 
Sbjct: 122 EACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVS 181

Query: 228 FTSLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGV 287
           F  +  E K  A   G+  Y+WDEF +LG+    ++   +  DICTIMYTSGT+G PKGV
Sbjct: 182 FGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGV 241

Query: 288 VLTHETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYH 347
           ++++E++VT I GV   ++   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ 
Sbjct: 242 MISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWR 301

Query: 348 GDLNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNR 407
           GD+  + EDL ELKPT+   VPRV +R+  G +  L     L++  FD  + YK  +M +
Sbjct: 302 GDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKK 361

Query: 408 GYKQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLT 467
           G    EASPL D L F KVK  LGG +R+I+SG A L+S VE FLRV  C   +QGYGLT
Sbjct: 362 GQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLT 421

Query: 468 ETCGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTE 527
           E+C  T +  PDE+ MLGTVG   P  D+RLE VPEM Y+ L     GEIC+RGK++F+ 
Sbjct: 422 ESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSG 481

Query: 528 YYKNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTN 587
           YYK  +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y  
Sbjct: 482 YYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGE 541

Query: 588 TPIVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELT 647
              V+ +W+YGNSF+S L+A+   ++   ++WA  NG    +  L   E+ + ++L EL 
Sbjct: 542 VQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELV 601

Query: 648 STAERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 705
             A+  K+K FE IK + L+  PFD+E DL+T T KKKR  LLK+Y+  I+E YK + AK
Sbjct: 602 KMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of Cp4.1LG03g05050 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.9e-191
Identity = 323/652 (49.54%), Postives = 435/652 (66.72%), Query Frame = 0

Query: 51  KRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQML 110
           KRF  +VE  +E  DG PS+GPVYR+  A++G P+  + + + WD+F  + EKY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 111 GWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEAC 170
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G K G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 171 NAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTS 230
           NAH + CVPLYDTLG  AV FII HAE+ I FV++KK  EL K+   + + +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 231 LKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLT 290
           +K E K  A   G+  +SWDEF +LG+    E+   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 291 HETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 350
           +E++VT   GV  ++      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 351 NAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNRGYK 410
             + EDL ELKP++   VPRV +R+  G +  L      ++K FD+ + YK   M +G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 411 QKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETC 470
              ASP  D L F KVK  LGG +R+I+SG A L+S +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 471 GPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYK 530
             T   FPDE+ MLGTVG   P  D+RLE VPEM Y+ LG  P GEIC+RGK++F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 531 NPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPI 590
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVY+   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 591 VEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTA 650
           +E IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +    + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 651 ERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYK 700
           + NKLK FE IK V LE   FD+E DL+T T KKKR  LLK+Y+  I+E YK
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYK 656

BLAST of Cp4.1LG03g05050 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 1.0e-189
Identity = 312/652 (47.85%), Postives = 445/652 (68.25%), Query Frame = 0

Query: 52  RFSIKVEEGREGKD-GKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQML 111
           R+ ++VE+G++G D G PS+GPVYR++ AKDG P   +DL ++WD+F +S EK     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 112 GWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEAC 171
           G R+ V+GK G Y+W+TYKEV+  V+ +G+++R +G   G + GIYG N P+WII+MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 172 NAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTS 231
           NAH + CVPLYDTLG  A+ FII HAE+ + F ++ K  ELLK+   + + LK +V F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 232 LKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLT 291
           +    ++ A  + +  YSWD+F +LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 292 HETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 351
           +E+++  + GV   ++  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 352 NAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNRGYK 411
             + ED+  LKPT+   VPRV ERI  G +  L     +++K F+  ++YK   M +G  
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 412 QKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETC 471
            ++ASP+AD + F+KVK  LGG +RLI+SG A L++ +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 472 GPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYK 531
           G T +  P+E+ MLGTVG   P  D+RLE VPEMGY+ L   P GEIC+RGK++F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 532 NPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPI 591
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+Y++   
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 592 VEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTA 651
           +E IW+YGNS++S LVAVV   +   + WA  +     F  +   ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604

Query: 652 ERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYK 700
           +  KLK FE IKGV L++ PFD+E DL+T + K KR  LLK+Y+ +I+E YK
Sbjct: 605 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

BLAST of Cp4.1LG03g05050 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.2e-172
Identity = 298/656 (45.43%), Postives = 415/656 (63.26%), Query Frame = 0

Query: 53  FSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQMLGW 112
           +++KVEE R   +  PS GPVYR++ A+DG   L E L + W+  S S ++     MLG 
Sbjct: 3   YTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLGR 62

Query: 113 RKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEACNA 172
           R+  + + GPY+W TY+EV+ + + + SA+R+ G  PG R GIYG NCPQWI+AM+AC +
Sbjct: 63  RQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQACYS 122

Query: 173 HSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTSLK 232
           H++  VPLYDTLG  AV FII+H E+ I FVQ+ K   +L         LK +V F ++ 
Sbjct: 123 HAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGNIT 182

Query: 233 EEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLTHE 292
              K      GV  +SW+EF +LG  +  E+      DICT+MYTSG +G PKGV+LT+E
Sbjct: 183 SVQKKETEALGVSCFSWEEFSQLGNLS-GELPEKHRTDICTLMYTSGATGEPKGVILTNE 242

Query: 293 TLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNA 352
            ++  I   D  +E  +   +E+DVY S+LPLAH+ D+ VE Y   KG+++GY+ GD+  
Sbjct: 243 AIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDIRF 302

Query: 353 IKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNRGYKQK 412
           + +DL ELKPT+  GVPRV++RI  G    +     L++  F   Y YK++ M +G    
Sbjct: 303 LMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLPHG 362

Query: 413 EASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETCGP 472
           +A+PL D L F K+K   GGR+RL+ SG A L   VEE+LRVT CA   QGYGLTE+CG 
Sbjct: 363 QAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESCGG 422

Query: 473 TTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYKNP 532
                 +   M+GTVG      + RLE VPEMGY+ L D+P GEIC+RG ++F+ Y+K  
Sbjct: 423 CLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHKRE 482

Query: 533 ELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPIVE 592
           +LTK+ + DGWFHTGDIGE  PNG +KIIDRKKN+ KLSQGEY+ +E +E +Y   P++ 
Sbjct: 483 DLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPLIA 542

Query: 593 DIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTAER 652
            IW+YGNSF+S LVAVVV      + WA +      F  L    +   Y+L EL STA++
Sbjct: 543 SIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTAKK 602

Query: 653 NKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 706
           ++L+ FE +K V LE +PFD+E DL+T T K KR  LLK+Y+  +++ Y   +  R
Sbjct: 603 HQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKEAR 657

BLAST of Cp4.1LG03g05050 vs. NCBI nr
Match: XP_023527772.1 (long chain acyl-CoA synthetase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1409 bits (3648), Expect = 0.0
Identity = 699/708 (98.73%), Postives = 701/708 (99.01%), Query Frame = 0

Query: 1   MCVCFHIPLAIILNSIHHCSTTSPHLTLLALHDFSFLFLLIPLFSRSSRMKRFSIKVEEG 60
           MCVCFHIPLAIILNSIHHCSTTSPHLTLLALHDFSFLFLLIPLFSRSSRMKRFSIKVEEG
Sbjct: 1   MCVCFHIPLAIILNSIHHCSTTSPHLTLLALHDFSFLFLLIPLFSRSSRMKRFSIKVEEG 60

Query: 61  REGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQMLGWRKFVEGKP 120
           REGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQMLGWRKFVEGKP
Sbjct: 61  REGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQMLGWRKFVEGKP 120

Query: 121 GPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEACNAHSMICVPL 180
           GPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEACNAHSMICVPL
Sbjct: 121 GPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEACNAHSMICVPL 180

Query: 181 YDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTSLKEEDKISAA 240
           YDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTSLKEEDKISAA
Sbjct: 181 YDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTSLKEEDKISAA 240

Query: 241 DNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLTHETLVTFIRG 300
           DNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLTHETLVTFIRG
Sbjct: 241 DNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLTHETLVTFIRG 300

Query: 301 VDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNAIKEDLMEL 360
           VDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNAIKEDLMEL
Sbjct: 301 VDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLNAIKEDLMEL 360

Query: 361 KPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGYKQKEASPLADF 420
           KPTLIVGVPRVFERIQEG K  + +L   RRKAFDLLYRYKLSWMNRGYKQKEASPLADF
Sbjct: 361 KPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYRYKLSWMNRGYKQKEASPLADF 420

Query: 421 LAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETCGPTTIGFPDE 480
           LAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETCGPTTIGFPDE
Sbjct: 421 LAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETCGPTTIGFPDE 480

Query: 481 MCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYKNPELTKEAIK 540
           MCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYKNPELTKEAIK
Sbjct: 481 MCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYKNPELTKEAIK 540

Query: 541 DGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPIVEDIWIYGNS 600
           DGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPIVEDIWIYGNS
Sbjct: 541 DGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPIVEDIWIYGNS 600

Query: 601 FKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTAERNKLKRFEY 660
           FKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTAERNKLKRFEY
Sbjct: 601 FKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTAERNKLKRFEY 660

Query: 661 IKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           IKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 661 IKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 708

BLAST of Cp4.1LG03g05050 vs. NCBI nr
Match: KAG6581311.1 (Long chain acyl-CoA synthetase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1302 bits (3369), Expect = 0.0
Identity = 640/659 (97.12%), Postives = 648/659 (98.33%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFSIKVEEGREGKDGKPSIGPVYRNV+AKDGSPSLFEDLNTSWDLF VSAEKYAGRQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSIGPVYRNVIAKDGSPSLFEDLNTSWDLFRVSAEKYAGRQM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFV+GKPGPYIWKTYKEVYGDVLNIGSALRAVG KPGSRIGIYGENCPQWIIAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAVGVKPGSRIGIYGENCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN IKEDLMELKPTLIVGVPRVFERIQEG K  + +L   RRKAFDLLYRYKLSWMNRGY
Sbjct: 301 LNGIKEDLMELKPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYRYKLSWMNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRN+VLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNHVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           A+RNKLKRFEYIKGVVLESHPFDIE D+VTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 601 ADRNKLKRFEYIKGVVLESHPFDIEKDMVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. NCBI nr
Match: XP_022934394.1 (long chain acyl-CoA synthetase 1-like [Cucurbita moschata])

HSP 1 Score: 1301 bits (3366), Expect = 0.0
Identity = 641/659 (97.27%), Postives = 647/659 (98.18%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFSIKVEEGREGKDGKPSIGPVYRNV+AKDGSPSLFEDLNTSWDLF VSAEKYAGRQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSIGPVYRNVIAKDGSPSLFEDLNTSWDLFRVSAEKYAGRQM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFV+GKPGPYIWKTYKEVYGDVLNIGSALRAVG KPGSRIGIYGENCPQWIIAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAVGVKPGSRIGIYGENCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEEDKISAADNGVK YSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEDKISAADNGVKTYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN IKEDLMELKPTLIVGVPRVFERIQEG K  + +L   RRKAFDLLYRYKLSWMNRGY
Sbjct: 301 LNGIKEDLMELKPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYRYKLSWMNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVY+NTP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           AERNKLKRFEYIKGVVLESHPFDIE D+VTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDMVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. NCBI nr
Match: XP_022983631.1 (long chain acyl-CoA synthetase 1-like [Cucurbita maxima] >XP_022983632.1 long chain acyl-CoA synthetase 1-like [Cucurbita maxima])

HSP 1 Score: 1298 bits (3360), Expect = 0.0
Identity = 638/659 (96.81%), Postives = 650/659 (98.63%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFSIKVEEGREGKDGKPS+GPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSVGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFVEGKPGPYIWKTYKEVYGDVL+IGSALRAVGAKPGSRIGIYGENCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYGDVLDIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKS RTNP+RLKVVVCFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSGRTNPDRLKVVVCFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEE+KISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THET+VTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETIVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LNAIKEDLMEL+PTLIVGVPRVFERIQEG K  + +L   RRKAFDLLY+YKLSWMNRGY
Sbjct: 301 LNAIKEDLMELRPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLGTVGSVTPFNDLRLE+VPEMGYNPLGDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEKVPEMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVY+NTP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           AERNKLKRFEYIKGVVLE HPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 601 AERNKLKRFEYIKGVVLEPHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. NCBI nr
Match: XP_016900385.1 (PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo] >XP_016900386.1 PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo])

HSP 1 Score: 1190 bits (3079), Expect = 0.0
Identity = 578/658 (87.84%), Postives = 618/658 (93.92%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFS+KVEEG+EGKDG+PS+GPVYRNVLA+   PS +EDL+TSWDLFSVSA+KYA R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRA+GA+PGSRIGIYG NCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHS+ICVPLYDTLGH AVNFIIDHAEIDI+F+QDKKA ELL SE   P+RLKVV+CFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEE+KISA +NG+KPYSWDEF RLGKENPSEI+PPR LDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           T ET+ TFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LNAIK+DLMELKPTL+VGVPRVFE+I EG K  + +L   RRKAFDLLY+YKLSWMN GY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQK+ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLG VG VT FN+LRLEEVP+MGYNPLGDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           K+PELTKE+IKDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEYIALEYLENVY+ TP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKS LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR+YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 704
           AERNKLKRFEYIKGVVLESHPFDIE DLVTATLKKKRNNLLKHYE QIN EYKNLE K
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETK 658

BLAST of Cp4.1LG03g05050 vs. ExPASy TrEMBL
Match: A0A6J1F2G0 (long chain acyl-CoA synthetase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441583 PE=4 SV=1)

HSP 1 Score: 1301 bits (3366), Expect = 0.0
Identity = 641/659 (97.27%), Postives = 647/659 (98.18%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFSIKVEEGREGKDGKPSIGPVYRNV+AKDGSPSLFEDLNTSWDLF VSAEKYAGRQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSIGPVYRNVIAKDGSPSLFEDLNTSWDLFRVSAEKYAGRQM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFV+GKPGPYIWKTYKEVYGDVLNIGSALRAVG KPGSRIGIYGENCPQWIIAMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAVGVKPGSRIGIYGENCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEEDKISAADNGVK YSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEDKISAADNGVKTYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN IKEDLMELKPTLIVGVPRVFERIQEG K  + +L   RRKAFDLLYRYKLSWMNRGY
Sbjct: 301 LNGIKEDLMELKPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYRYKLSWMNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVY+NTP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           AERNKLKRFEYIKGVVLESHPFDIE D+VTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDMVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. ExPASy TrEMBL
Match: A0A6J1J2W0 (long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482186 PE=4 SV=1)

HSP 1 Score: 1298 bits (3360), Expect = 0.0
Identity = 638/659 (96.81%), Postives = 650/659 (98.63%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFSIKVEEGREGKDGKPS+GPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM
Sbjct: 1   MKRFSIKVEEGREGKDGKPSVGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFVEGKPGPYIWKTYKEVYGDVL+IGSALRAVGAKPGSRIGIYGENCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYGDVLDIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKS RTNP+RLKVVVCFT
Sbjct: 121 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSGRTNPDRLKVVVCFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEE+KISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THET+VTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 THETIVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LNAIKEDLMEL+PTLIVGVPRVFERIQEG K  + +L   RRKAFDLLY+YKLSWMNRGY
Sbjct: 301 LNAIKEDLMELRPTLIVGVPRVFERIQEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET
Sbjct: 361 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLGTVGSVTPFNDLRLE+VPEMGYNPLGDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEKVPEMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVY+NTP
Sbjct: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSNTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           AERNKLKRFEYIKGVVLE HPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR
Sbjct: 601 AERNKLKRFEYIKGVVLEPHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. ExPASy TrEMBL
Match: A0A1S4DWM9 (long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV=1)

HSP 1 Score: 1190 bits (3079), Expect = 0.0
Identity = 578/658 (87.84%), Postives = 618/658 (93.92%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFS+KVEEG+EGKDG+PS+GPVYRNVLA+   PS +EDL+TSWDLFSVSA+KYA R+M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRA+GA+PGSRIGIYG NCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHS+ICVPLYDTLGH AVNFIIDHAEIDI+F+QDKKA ELL SE   P+RLKVV+CFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEE+KISA +NG+KPYSWDEF RLGKENPSEI+PPR LDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           T ET+ TFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LNAIK+DLMELKPTL+VGVPRVFE+I EG K  + +L   RRKAFDLLY+YKLSWMN GY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQK+ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLG VG VT FN+LRLEEVP+MGYNPLGDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           K+PELTKE+IKDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEYIALEYLENVY+ TP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKS LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR+YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 704
           AERNKLKRFEYIKGVVLESHPFDIE DLVTATLKKKRNNLLKHYE QIN EYKNLE K
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLETK 658

BLAST of Cp4.1LG03g05050 vs. ExPASy TrEMBL
Match: A0A0A0L8J2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G535120 PE=4 SV=1)

HSP 1 Score: 1184 bits (3064), Expect = 0.0
Identity = 578/658 (87.84%), Postives = 615/658 (93.47%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MKRFS+KVEEGREGKDGKPS+GPVYRNVLA+   PS +EDL+TSWDLFSVSA KYA R+M
Sbjct: 1   MKRFSVKVEEGREGKDGKPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSANKYAERRM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFV+GKPGPYIWKTYKEVYGDVLNIGSALRA+GA+PGSRIGIYG NCPQWI+AMEA
Sbjct: 61  LGWRKFVDGKPGPYIWKTYKEVYGDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHS+ICVPLYDTLGH AVNFIIDHAEIDIVF+QDKKAKELL S  T PERLKV +CFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFIQDKKAKELLNSGCTIPERLKVFICFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLKEE+KISA +NG+KPYSWDEF RLGKENPSEI+PPR LDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           T ET+ TFIRGVDI MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           L+AIK+DLMELKPTL+VGVPRVFE+I EG K  + +L   RRKAFDLLY+YKLSWMN GY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQK+ASPLAD LAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFF+QGYGLTET
Sbjct: 361 KQKDASPLADLLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTTIGFPDEMCMLG VG VT FN+L LEEVP+MGYNPLGDRPCGEICVRGK+VFTEYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELCLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           K+PELTKE+IKDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEYIALEYLENVY+ TP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKS LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR+YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 704
           AERNKLKRFEYIKGVVLE  PFDIE DLVTATLKKKRNNLLKHYEVQIN EYKNLE K
Sbjct: 601 AERNKLKRFEYIKGVVLELRPFDIEKDLVTATLKKKRNNLLKHYEVQINAEYKNLEKK 658

BLAST of Cp4.1LG03g05050 vs. ExPASy TrEMBL
Match: A0A6J1K7Y2 (long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493051 PE=4 SV=1)

HSP 1 Score: 1177 bits (3044), Expect = 0.0
Identity = 567/659 (86.04%), Postives = 614/659 (93.17%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MK FSIKVEEG EGKDGKPS+GPVYRNVL KDG P L+EDL+T+WD+FSVSA+KYAG++M
Sbjct: 1   MKSFSIKVEEGTEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWRKFVEGKPGPYIWKTY+EVY DVLNIGSALRAVGAK GSRIGIYG NCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           CNAHS++ VPLYDTLGH AVN+II+HAEIDI+F+QDKKAKELL SE TNP+RLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSESTNPKRLKVIICFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           SLK E+K +A  NG+KPYSWDEF RLGKE+P EI PPR LD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKAAAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           THET+ TFIRGVD++MEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           L+AIK+DLMELKPTL+VGVPRVFE+I EG K  + +L   RRKAFDLLY+YKLSWMN GY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
           KQK+ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA F+QGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
           CGPTT+GFPDEMCMLG VG+VT +N+LRLEEVPEMGYNPLGDRPCGEICVRGK+VF EYY
Sbjct: 421 CGPTTVGFPDEMCMLGKVGAVTLYNELRLEEVPEMGYNPLGDRPCGEICVRGKTVFNEYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELTKE+IKDGWFHTGDIGEI PNGVVKIIDRKKNLIKLSQGEYIALEYLENVY+ TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           IVEDIWIYGNSFKS+LVAVVVLHEENTKKWANSNGFLCSFSEL  LEQLR YVL ELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLFELTST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 705
           AER KLKRFEYIKGVVLES PFDIE++LVTATLKKKRNNLLKHYE +IN EYKNLEAKR
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLLKHYEKEINAEYKNLEAKR 659

BLAST of Cp4.1LG03g05050 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 914.8 bits (2363), Expect = 4.1e-266
Identity = 423/659 (64.19%), Postives = 532/659 (80.73%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MK F+ KVEEG +G DGKPS+GPVYRN+L++ G P +  ++ T+WD+FS S EK+    M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA GA+PGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           C AH++ICVPLYDTLG  AV++I++HAEID VFVQD K K LL+ +    +RLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           ++ +E    A++ GVK YSW +F  +G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           TH+ + TF+ G+D+YM+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN +++D+ ELKPT + GVPRVFERI EG +  L  L   RR  F+ LY++KL+W+NRGY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
              +ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F +QGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
            G T +GFPDEMCMLGTVG    +N++RLEEV EMGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELT+E +KDGWFHTGDIGEILPNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           +V+DIW+YG+SFKS LVAVVV + E   +WA   GF   F EL S  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 706
           AE+NKL++FEYIK V +E+ PFD+E DLVTATLK +RNNLLK+Y+VQI+E Y+ L +K+
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Cp4.1LG03g05050 vs. TAIR 10
Match: AT2G47240.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 914.8 bits (2363), Expect = 4.1e-266
Identity = 423/659 (64.19%), Postives = 532/659 (80.73%), Query Frame = 0

Query: 50  MKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQM 109
           MK F+ KVEEG +G DGKPS+GPVYRN+L++ G P +  ++ T+WD+FS S EK+    M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 110 LGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEA 169
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRA GA+PGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 170 CNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFT 229
           C AH++ICVPLYDTLG  AV++I++HAEID VFVQD K K LL+ +    +RLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 230 SLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVL 289
           ++ +E    A++ GVK YSW +F  +G+E P +  PP+  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 290 THETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 349
           TH+ + TF+ G+D+YM+QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 350 LNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLVIL---RRKAFDLLYRYKLSWMNRGY 409
           LN +++D+ ELKPT + GVPRVFERI EG +  L  L   RR  F+ LY++KL+W+NRGY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 410 KQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTET 469
              +ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F +QGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 470 CGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYY 529
            G T +GFPDEMCMLGTVG    +N++RLEEV EMGY+PLG+ P GEIC+RG+ +F+ YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 530 KNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTP 589
           KNPELT+E +KDGWFHTGDIGEILPNGV+KIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 590 IVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTST 649
           +V+DIW+YG+SFKS LVAVVV + E   +WA   GF   F EL S  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 650 AERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAKR 706
           AE+NKL++FEYIK V +E+ PFD+E DLVTATLK +RNNLLK+Y+VQI+E Y+ L +K+
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Cp4.1LG03g05050 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 693.0 bits (1787), Expect = 2.6e-199
Identity = 328/660 (49.70%), Postives = 451/660 (68.33%), Query Frame = 0

Query: 48  SRMKRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGR 107
           S+ K++  +VEEG+EG DG+PS+GPVYR++ AKDG P   E +++ WD+F +S EKY   
Sbjct: 2   SQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNN 61

Query: 108 QMLGWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAM 167
            MLG R+ V+GKPG Y+W+TY+EVY  V+ +G++LR+VG K  ++ GIYG N P+WII+M
Sbjct: 62  PMLGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISM 121

Query: 168 EACNAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVC 227
           EACNAH + CVPLYDTLG +AV FII H+E+ IVFV++KK  EL K+   + E +K VV 
Sbjct: 122 EACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVS 181

Query: 228 FTSLKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGV 287
           F  +  E K  A   G+  Y+WDEF +LG+    ++   +  DICTIMYTSGT+G PKGV
Sbjct: 182 FGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGV 241

Query: 288 VLTHETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYH 347
           ++++E++VT I GV   ++   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ 
Sbjct: 242 MISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWR 301

Query: 348 GDLNAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNR 407
           GD+  + EDL ELKPT+   VPRV +R+  G +  L     L++  FD  + YK  +M +
Sbjct: 302 GDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKK 361

Query: 408 GYKQKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLT 467
           G    EASPL D L F KVK  LGG +R+I+SG A L+S VE FLRV  C   +QGYGLT
Sbjct: 362 GQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLT 421

Query: 468 ETCGPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTE 527
           E+C  T +  PDE+ MLGTVG   P  D+RLE VPEM Y+ L     GEIC+RGK++F+ 
Sbjct: 422 ESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSG 481

Query: 528 YYKNPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTN 587
           YYK  +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y  
Sbjct: 482 YYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGE 541

Query: 588 TPIVEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELT 647
              V+ +W+YGNSF+S L+A+   ++   ++WA  NG    +  L   E+ + ++L EL 
Sbjct: 542 VQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELV 601

Query: 648 STAERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYKNLEAK 705
             A+  K+K FE IK + L+  PFD+E DL+T T KKKR  LLK+Y+  I+E YK + AK
Sbjct: 602 KMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of Cp4.1LG03g05050 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-192
Identity = 323/652 (49.54%), Postives = 435/652 (66.72%), Query Frame = 0

Query: 51  KRFSIKVEEGREGKDGKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQML 110
           KRF  +VE  +E  DG PS+GPVYR+  A++G P+  + + + WD+F  + EKY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 111 GWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEAC 170
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G K G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 171 NAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTS 230
           NAH + CVPLYDTLG  AV FII HAE+ I FV++KK  EL K+   + + +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 231 LKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLT 290
           +K E K  A   G+  +SWDEF +LG+    E+   +  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 291 HETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 350
           +E++VT   GV  ++      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 351 NAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNRGYK 410
             + EDL ELKP++   VPRV +R+  G +  L      ++K FD+ + YK   M +G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 411 QKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETC 470
              ASP  D L F KVK  LGG +R+I+SG A L+S +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 471 GPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYK 530
             T   FPDE+ MLGTVG   P  D+RLE VPEM Y+ LG  P GEIC+RGK++F+ YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 531 NPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPI 590
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVY+   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 591 VEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTA 650
           +E IW+YGNSF+S LVA+    ++  ++WA  NG    F+ +    + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 651 ERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYK 700
           + NKLK FE IK V LE   FD+E DL+T T KKKR  LLK+Y+  I+E YK
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYK 656

BLAST of Cp4.1LG03g05050 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 664.8 bits (1714), Expect = 7.5e-191
Identity = 312/652 (47.85%), Postives = 445/652 (68.25%), Query Frame = 0

Query: 52  RFSIKVEEGREGKD-GKPSIGPVYRNVLAKDGSPSLFEDLNTSWDLFSVSAEKYAGRQML 111
           R+ ++VE+G++G D G PS+GPVYR++ AKDG P   +DL ++WD+F +S EK     ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 112 GWRKFVEGKPGPYIWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGENCPQWIIAMEAC 171
           G R+ V+GK G Y+W+TYKEV+  V+ +G+++R +G   G + GIYG N P+WII+MEAC
Sbjct: 65  GRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 124

Query: 172 NAHSMICVPLYDTLGHEAVNFIIDHAEIDIVFVQDKKAKELLKSERTNPERLKVVVCFTS 231
           NAH + CVPLYDTLG  A+ FII HAE+ + F ++ K  ELLK+   + + LK +V F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 184

Query: 232 LKEEDKISAADNGVKPYSWDEFFRLGKENPSEITPPRLLDICTIMYTSGTSGTPKGVVLT 291
           +    ++ A  + +  YSWD+F +LG+    E+   R  D+CTIMYTSGT+G PKGV+LT
Sbjct: 185 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 244

Query: 292 HETLVTFIRGVDIYMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 351
           +E+++  + GV   ++  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+
Sbjct: 245 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 304

Query: 352 NAIKEDLMELKPTLIVGVPRVFERIQEGTKTLLV---ILRRKAFDLLYRYKLSWMNRGYK 411
             + ED+  LKPT+   VPRV ERI  G +  L     +++K F+  ++YK   M +G  
Sbjct: 305 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 364

Query: 412 QKEASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFIQGYGLTETC 471
            ++ASP+AD + F+KVK  LGG +RLI+SG A L++ +E FLRV  CA  +QGYGLTE+C
Sbjct: 365 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 424

Query: 472 GPTTIGFPDEMCMLGTVGSVTPFNDLRLEEVPEMGYNPLGDRPCGEICVRGKSVFTEYYK 531
           G T +  P+E+ MLGTVG   P  D+RLE VPEMGY+ L   P GEIC+RGK++F+ YYK
Sbjct: 425 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 484

Query: 532 NPELTKEAIKDGWFHTGDIGEILPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYTNTPI 591
             +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+Y++   
Sbjct: 485 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 544

Query: 592 VEDIWIYGNSFKSRLVAVVVLHEENTKKWANSNGFLCSFSELSSLEQLRNYVLSELTSTA 651
           +E IW+YGNS++S LVAVV   +   + WA  +     F  +   ++ + +VL E    A
Sbjct: 545 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 604

Query: 652 ERNKLKRFEYIKGVVLESHPFDIENDLVTATLKKKRNNLLKHYEVQINEEYK 700
           +  KLK FE IKGV L++ PFD+E DL+T + K KR  LLK+Y+ +I+E YK
Sbjct: 605 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O228985.8e-26564.19Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 S... [more]
Q9T0A03.6e-19849.70Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9T0091.9e-19149.54Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Q9C7W41.0e-18947.85Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
M4ISH11.2e-17245.43Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023527772.10.098.73long chain acyl-CoA synthetase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6581311.10.097.12Long chain acyl-CoA synthetase 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022934394.10.097.27long chain acyl-CoA synthetase 1-like [Cucurbita moschata][more]
XP_022983631.10.096.81long chain acyl-CoA synthetase 1-like [Cucurbita maxima] >XP_022983632.1 long ch... [more]
XP_016900385.10.087.84PREDICTED: long chain acyl-CoA synthetase 1 [Cucumis melo] >XP_016900386.1 PREDI... [more]
Match NameE-valueIdentityDescription
A0A6J1F2G00.097.27long chain acyl-CoA synthetase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A6J1J2W00.096.81long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148218... [more]
A0A1S4DWM90.087.84long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV... [more]
A0A0A0L8J20.087.84AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G535120... [more]
A0A6J1K7Y20.086.04long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149305... [more]
Match NameE-valueIdentityDescription
AT2G47240.14.1e-26664.19AMP-dependent synthetase and ligase family protein [more]
AT2G47240.24.1e-26664.19AMP-dependent synthetase and ligase family protein [more]
AT4G23850.12.6e-19949.70AMP-dependent synthetase and ligase family protein [more]
AT4G11030.11.4e-19249.54AMP-dependent synthetase and ligase family protein [more]
AT1G64400.17.5e-19147.85AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 117..568
e-value: 2.4E-104
score: 349.3
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 85..560
e-value: 5.4E-86
score: 290.7
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 50..702
NoneNo IPR availablePANTHERPTHR43272:SF6LONG CHAIN ACYL-COA SYNTHETASE 1coord: 50..702
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 121..699
e-value: 0.0
score: 745.192
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 89..698
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 274..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g05050.1Cp4.1LG03g05050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010143 cutin biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
biological_process GO:0010025 wax biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity