Cp4.1LG02g00860 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g00860
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	AAI domain-containing protein
Location	Cp4.1LG02: 5115094 .. 5115399 (+)
RNA-Seq Expression	Cp4.1LG02g00860
Synteny	Cp4.1LG02g00860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCCAACAAGGTTCTGGTGGCAGTTCTGTTCTTGATCGCTGTCGGTGGCGTCCTCGCATCTCCTCTGTGTAAGATCGATACCACTGACCTCGGCGTATGTCGCCCGGCAGTTACCACTCCGGAGCATGGCCAGCCTCTGCCATTGCCAACAGAAGGCTGCTGTGCTGTGGTTCGCCGTGCTGACCTTAAGTGTCTTTGCAATCTCAAGTCTATGCTCCCTGCCATTGGTATCGACGCTGCAAATGCCATGGCTTTGCCTGGTAAATGTGGTCTATCGATCCCACCGGAGTGTCATCTTTAG

mRNA sequence

ATGGCTCCCAACAAGGTTCTGGTGGCAGTTCTGTTCTTGATCGCTGTCGGTGGCGTCCTCGCATCTCCTCTGTGTAAGATCGATACCACTGACCTCGGCGTATGTCGCCCGGCAGTTACCACTCCGGAGCATGGCCAGCCTCTGCCATTGCCAACAGAAGGCTGCTGTGCTGTGGTTCGCCGTGCTGACCTTAAGTGTCTTTGCAATCTCAAGTCTATGCTCCCTGCCATTGGTATCGACGCTGCAAATGCCATGGCTTTGCCTGGTAAATGTGGTCTATCGATCCCACCGGAGTGTCATCTTTAG

Coding sequence (CDS)

ATGGCTCCCAACAAGGTTCTGGTGGCAGTTCTGTTCTTGATCGCTGTCGGTGGCGTCCTCGCATCTCCTCTGTGTAAGATCGATACCACTGACCTCGGCGTATGTCGCCCGGCAGTTACCACTCCGGAGCATGGCCAGCCTCTGCCATTGCCAACAGAAGGCTGCTGTGCTGTGGTTCGCCGTGCTGACCTTAAGTGTCTTTGCAATCTCAAGTCTATGCTCCCTGCCATTGGTATCGACGCTGCAAATGCCATGGCTTTGCCTGGTAAATGTGGTCTATCGATCCCACCGGAGTGTCATCTTTAG

Protein sequence

MAPNKVLVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECHL

Homology

[Close]

BLAST of Cp4.1LG02g00860 vs. NCBI nr
Match: KAG7037349.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 209 bits (531), Expect = 4.78e-68
Identity = 100/101 (99.01%), Postives = 100/101 (99.01%), Query Frame = 0


Query: 1   MAPNKVLVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVR 60
           MAPNKVLVA LFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVR
Sbjct: 1   MAPNKVLVAALFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVR 60

Query: 61  RADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECHL 101
           RADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECHL
Sbjct: 61  RADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECHL 101

[Close]

BLAST of Cp4.1LG02g00860 vs. NCBI nr
Match: XP_008447695.1 (PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo] >KAA0048163.1 putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (362), Expect = 3.04e-42
Identity = 67/102 (65.69%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 1   MAPNKV--LVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAV 60
           MAP+K+  L+ +L L+  G V +SPLC+IDTTDL VCRPAVT P+HG+ LPLPT+ CC+V
Sbjct: 1   MAPSKLHLLLLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSV 60

Query: 61  VRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           VRRADLKCLC+LK  LP+ GI+ ANA+ALP KCG+S PPEC 
Sbjct: 61  VRRADLKCLCSLKYALPSFGINTANALALPSKCGVSPPPECR 102

[Close]

BLAST of Cp4.1LG02g00860 vs. NCBI nr
Match: KAG6607773.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 131 bits (329), Expect = 2.79e-37
Identity = 59/96 (61.46%), Postives = 77/96 (80.21%), Query Frame = 0


Query: 5   KVLVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADL 64
           K L ++LF + + G +A+PLC++DT +L +C+PAV  P HGQPL  PT+GCC+VVRRADL
Sbjct: 3   KFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPP-HGQPLQPPTKGCCSVVRRADL 62

Query: 65  KCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           KCLC+LKS+LP IGI+ ANA+ALP KCG+  PPEC 
Sbjct: 63  KCLCSLKSVLPTIGINTANALALPSKCGIHTPPECR 97

[Close]

BLAST of Cp4.1LG02g00860 vs. NCBI nr
Match: KAE8646001.1 (hypothetical protein Csa_015500 [Cucumis sativus])

HSP 1 Score: 131 bits (330), Expect = 3.27e-37
Identity = 63/93 (67.74%), Postives = 76/93 (81.72%), Query Frame = 0


Query: 1  MAPNKV--LVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAV 60
          MAP+K+  L+ +L L+    V A PLCKIDT+DL +CR AVT P+HGQPLPLPTE CC+V
Sbjct: 1  MAPSKLHLLLPLLVLLLPAAVTAYPLCKIDTSDLKLCRSAVTPPDHGQPLPLPTEDCCSV 60

Query: 61 VRRADLKCLCNLKSMLPAIGIDAANAMALPGKC 91
          VR ADLKCLCNLKS+LP++GID ANA+ALP KC
Sbjct: 61 VRHADLKCLCNLKSVLPSMGIDTANALALPSKC 93

[Close]

BLAST of Cp4.1LG02g00860 vs. NCBI nr
Match: KAG7037350.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130 bits (327), Expect = 6.34e-37
Identity = 59/96 (61.46%), Postives = 77/96 (80.21%), Query Frame = 0


Query: 5   KVLVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADL 64
           K L ++LF + + G +A+PLC++DT +L +C+PAV  P HGQ L  PT+GCC+VVRRADL
Sbjct: 7   KFLASLLFSLFLVGTIATPLCRVDTKELKMCQPAVAPP-HGQLLQPPTKGCCSVVRRADL 66

Query: 65  KCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           KCLC+LKS+LP IGI+ ANA+ALP KCG+  PPECH
Sbjct: 67  KCLCSLKSVLPTIGINTANALALPSKCGIHTPPECH 101

[Close]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL
Match: A0A0A0LAS6 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G760500 PE=4 SV=1)

HSP 1 Score: 146 bits (368), Expect = 1.79e-43
Identity = 68/102 (66.67%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 1   MAPNKV--LVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAV 60
           MAP+K+  L+ +L L+    V A PLCKIDT+DL +CR AVT P+HGQPLPLPTE CC+V
Sbjct: 1   MAPSKLHLLLPLLVLLLPAAVTAYPLCKIDTSDLKLCRSAVTPPDHGQPLPLPTEDCCSV 60

Query: 61  VRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           VR ADLKCLCNLKS+LP++GID ANA+ALP KC ++ PPECH
Sbjct: 61  VRHADLKCLCNLKSVLPSMGIDTANALALPSKCNVASPPECH 102

[Close]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL
Match: A0A5A7TX28 (Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G00630 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 1.47e-42
Identity = 67/102 (65.69%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 1   MAPNKV--LVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAV 60
           MAP+K+  L+ +L L+  G V +SPLC+IDTTDL VCRPAVT P+HG+ LPLPT+ CC+V
Sbjct: 1   MAPSKLHLLLLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSV 60

Query: 61  VRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           VRRADLKCLC+LK  LP+ GI+ ANA+ALP KCG+S PPEC 
Sbjct: 61  VRRADLKCLCSLKYALPSFGINTANALALPSKCGVSPPPECR 102

[Close]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL
Match: A0A1S3BHE7 (putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103490109 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 1.47e-42
Identity = 67/102 (65.69%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 1   MAPNKV--LVAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAV 60
           MAP+K+  L+ +L L+  G V +SPLC+IDTTDL VCRPAVT P+HG+ LPLPT+ CC+V
Sbjct: 1   MAPSKLHLLLLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSV 60

Query: 61  VRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLSIPPECH 100
           VRRADLKCLC+LK  LP+ GI+ ANA+ALP KCG+S PPEC 
Sbjct: 61  VRRADLKCLCSLKYALPSFGINTANALALPSKCGVSPPPECR 102

[Close]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL
Match: A0A5A7TY68 (Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G00600 PE=4 SV=1)

HSP 1 Score: 114 bits (284), Expect = 7.00e-31
Identity = 53/83 (63.86%), Postives = 63/83 (75.90%), Query Frame = 0


Query: 18  GVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSMLPAI 77
           G  A+ LCK+DT  L VCRPAV  P HG+PL  PT+ CC+VVR ADLKCLCNLKS+LP+ 
Sbjct: 5   GTSATLLCKVDTKKLCVCRPAVA-PLHGKPLQPPTKRCCSVVRSADLKCLCNLKSILPSA 64

Query: 78  GIDAANAMALPGKCGLSIPPECH 100
           GI+A  A+ALP KCG+  PPEC 
Sbjct: 65  GINATIALALPSKCGIRTPPECR 86

[Close]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL
Match: A0A0A0LBB0 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G759990 PE=4 SV=1)

HSP 1 Score: 114 bits (285), Expect = 8.82e-31
Identity = 52/80 (65.00%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 21  ASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSMLPAIGID 80
           A+ LCK+DT +L VCRPAV  P HG+PL  PTE CC+V+R ADLKCLCNLKS+LP+ GI+
Sbjct: 28  ATLLCKVDTKELCVCRPAVA-PLHGKPLQPPTERCCSVLRSADLKCLCNLKSILPSAGIN 87

Query: 81  AANAMALPGKCGLSIPPECH 100
           A  A+ALP KCG+  PPEC 
Sbjct: 88  ATIALALPSKCGIPSPPECR 106

[Close]

BLAST of Cp4.1LG02g00860 vs. TAIR 10
Match: AT5G55450.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 3.1e-13
Identity = 38/83 (45.78%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 14  IAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSM 73
           I V    + P+C IDT DL  CRPAVT    G   P P   CCAV R A+L+CLC  K  
Sbjct: 23  IMVKEATSIPVCNIDTNDLAKCRPAVT----GNNPPPPGPDCCAVARVANLQCLCPYKPY 82

Query: 74  LPAIGIDAANAMALPGKCGLSIP 97
           LP +GID +    L   CG++ P
Sbjct: 83  LPTVGIDPSRVRPLLANCGVNSP 101

[Close]

BLAST of Cp4.1LG02g00860 vs. TAIR 10
Match: AT5G55410.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 5.8e-12
Identity = 34/95 (35.79%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 8   VAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCL 67
           + + F + V    +  +C +D  D+  CRPA+T    G   P P   CC VVR+A+ +CL
Sbjct: 17  MVLTFAMMVKEATSMSICDMDINDMQKCRPAIT----GNNPPPPVNDCCVVVRKANFECL 76

Query: 68  CNLKSMLPAIGIDAANAMALPGKCGL-SIPPECHL 102
           C  K  LP + ID +  +AL  KCG+ ++P  C +
Sbjct: 77  CRFKFYLPILRIDPSKVVALVAKCGVTTVPRSCQV 107

[Close]

BLAST of Cp4.1LG02g00860 vs. TAIR 10
Match: AT5G55410.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 7.5e-12
Identity = 34/93 (36.56%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 8   VAVLFLIAVGGVLASPLCKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCL 67
           + + F + V    +  +C +D  D+  CRPA+T    G   P P   CC VVR+A+ +CL
Sbjct: 17  MVLTFAMMVKEATSMSICDMDINDMQKCRPAIT----GNNPPPPVNDCCVVVRKANFECL 76

Query: 68  CNLKSMLPAIGIDAANAMALPGKCGL-SIPPEC 100
           C  K  LP + ID +  +AL  KCG+ ++P  C
Sbjct: 77  CRFKFYLPILRIDPSKVVALVAKCGVTTVPRSC 105

[Close]

BLAST of Cp4.1LG02g00860 vs. TAIR 10
Match: AT5G55460.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 4.9e-11
Identity = 32/76 (42.11%), Postives = 44/76 (57.89%), Query Frame = 0


Query: 25  CKIDTTDLGVCRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSMLPAIGIDAANA 84
           C I+   L  CRPAV     G   P P + CC +++ A+LKC+C  KS+LP + +  +  
Sbjct: 33  CNINANHLEKCRPAVI----GDNPPSPIKECCELLQAANLKCICRFKSVLPVLAVYPSKV 92

Query: 85  MALPGKCGL-SIPPEC 100
            AL  KCGL +IPP C
Sbjct: 93  QALLSKCGLTTIPPAC 104

[Close]

BLAST of Cp4.1LG02g00860 vs. TAIR 10
Match: AT3G52130.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.1e-05
Identity = 26/63 (41.27%), Postives = 33/63 (52.38%), Query Frame = 0


Query: 35  CRPAVTTPEHGQPLPLPTEGCCAVVRRADLKCLCNLKSMLPAIGIDAANAMALPGKCGLS 94
           CR AV       P+P PTE CC+ V      CLC L +  P  GID + A+ LP +C  +
Sbjct: 62  CRAAVAP---FSPIP-PTEICCSAVVTLGRPCLCLLANGPPLSGIDRSMALQLPQRCSAN 120

Query: 95  IPP 98
            PP
Sbjct: 122 FPP 120

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g00860 vs.


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG02g00860 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037349.1	4.78e-68	99.01	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_008447695.1	3.04e-42	65.69	PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo] >KAA0048163.1 put...	[more]
KAG6607773.1	2.79e-37	61.46	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sor...	[more]
KAE8646001.1	3.27e-37	67.74	hypothetical protein Csa_015500 [Cucumis sativus]	[more]
KAG7037350.1	6.34e-37	61.46	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. arg...	[more]

BLAST of Cp4.1LG02g00860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAS6	1.79e-43	66.67	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G760500 PE=4 SV...	[more]
A0A5A7TX28	1.47e-42	65.69	Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BHE7	1.47e-42	65.69	putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103490109 PE=...	[more]
A0A5A7TY68	7.00e-31	63.86	Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A0A0LBB0	8.82e-31	65.00	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G759990 PE=4 SV...	[more]

BLAST of Cp4.1LG02g00860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55450.1	3.1e-13	45.78	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55410.1	5.8e-12	35.79	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55410.2	7.5e-12	36.56	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G55460.1	4.9e-11	42.11	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G52130.1	3.1e-05	41.27	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 25..99 e-value: 1.2E-4 score: 31.5
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 49..99 e-value: 7.3E-9 score: 35.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 22..99 e-value: 2.3E-16 score: 61.4
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 25..99
IPR039265	Putative lipid-transfer protein DIR1-like	PANTHER	PTHR33122	LIPID BINDING PROTEIN-RELATED	coord: 6..100
None	No IPR available	PANTHER	PTHR33122:SF43	LIPID TRANSFER PROTEIN	coord: 6..100
IPR044741	Non-specific lipid-transfer protein-like	CDD	cd04660	nsLTP_like	coord: 25..97 e-value: 1.84546E-16 score: 65.8534

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g00860.1	Cp4.1LG02g00860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009627	systemic acquired resistance
molecular_function	GO:0005504	fatty acid binding