Cp4.1LG02g00790 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g00790
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationCp4.1LG02: 5080446 .. 5081492 (+)
RNA-Seq ExpressionCp4.1LG02g00790
SyntenyCp4.1LG02g00790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCGGATCTCTACACCTTACTCAAAATCTCCGCCGCCGTTCTTGCCTCTTTACTCTACTCCTACTTCATCTCCACCAAGCTCCCCAAAGGTCTCCCAAGGCTTCTCTCTCTCCTCCCAATCCTCTCTCTCTACACCCTCCTCCCTCTCCCTCTCTCCTCCCTCCTCCTCGCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTTCTGTTCTGCTTCGATTCCGGCCCTCTATTTACCGACCCACCATTGTCATTCCCTCTGTTCGTCTCAATCGCTTGTTTCCCGATCAGAATCAAGCAAAATCAGCCCGACCCAGATCCTCAAAATCCTCAAAAACCCACGGATCCACCGTTGAAGACCATCGATTCCCCCAAATTGCCGCTCAATCTGCCGGCGAAGGGGTTTCTCTTCGCCGTCTTGGTCGCCGGCGATGATTTCGCCCAAAATCTGTCCCCGAATGCTATGCTCTGTTTTTACTGTGTGTCTCTGTATTTGTTTATTGATGTTGTTTTGGGTCTGTCTAATACGTTTGTGAGATGGGGTTTTGGAATCGAGCTCGAACCGCCGTCGAACGAGCCGTATTTGGCGACGTCGCTCCGGGATTTTTGGGGGAGGAGATGGAATTTGTTGGTTTCGAATACTTTAAGATGGTCTGTATTTTTGCCGGTTAGGGCCGCCGTGAGCGGCGTTTTGGGGCGGCGACGGGCAGCGGTTGTGGCAGTGTTTGCGACGTTTGTGGTGTCGGGGCTGATGCATGAGCTGCTGTTTTACTATGTCACCGGAGCGGTTCCGACTTGGGAGGTTACTGGGTTTTTTGTGCTTCATGGGGTTTGTGTGGTGCTGGAATTTTGGGTGAAGAAGGCTTTGGGAGAAAATTGGCGGCTTCATTGGGCGGTTGCCGGGCCGTTGACGTTGGCGTTTGTGGTGATCACCGCCGCGTGGTTGTTCTTTCCGCCGCTGTTGAAGACTGGTGCGGTGGCCGCAGTTATCGGGGAATGTAAGGCGGCGGCGGGGTATGTGAAATGA

mRNA sequence

ATGGCGGCGGATCTCTACACCTTACTCAAAATCTCCGCCGCCGTTCTTGCCTCTTTACTCTACTCCTACTTCATCTCCACCAAGCTCCCCAAAGGTCTCCCAAGGCTTCTCTCTCTCCTCCCAATCCTCTCTCTCTACACCCTCCTCCCTCTCCCTCTCTCCTCCCTCCTCCTCGCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTTCTGTTCTGCTTCGATTCCGGCCCTCTATTTACCGACCCACCATTGTCATTCCCTCTGTTCGTCTCAATCGCTTGTTTCCCGATCAGAATCAAGCAAAATCAGCCCGACCCAGATCCTCAAAATCCTCAAAAACCCACGGATCCACCGTTGAAGACCATCGATTCCCCCAAATTGCCGCTCAATCTGCCGGCGAAGGGGTTTCTCTTCGCCGTCTTGGTCGCCGGCGATGATTTCGCCCAAAATCTGTCCCCGAATGCTATGCTCTGTTTTTACTGTGTGTCTCTGTATTTGTTTATTGATGTTGTTTTGGGTCTGTCTAATACGTTTGTGAGATGGGGTTTTGGAATCGAGCTCGAACCGCCGTCGAACGAGCCGTATTTGGCGACGTCGCTCCGGGATTTTTGGGGGAGGAGATGGAATTTGTTGGTTTCGAATACTTTAAGATGGTCTGTATTTTTGCCGGTTAGGGCCGCCGTGAGCGGCGTTTTGGGGCGGCGACGGGCAGCGGTTGTGGCAGTGTTTGCGACGTTTGTGGTGTCGGGGCTGATGCATGAGCTGCTGTTTTACTATGTCACCGGAGCGGTTCCGACTTGGGAGGTTACTGGGTTTTTTGTGCTTCATGGGGTTTGTGTGGTGCTGGAATTTTGGGTGAAGAAGGCTTTGGGAGAAAATTGGCGGCTTCATTGGGCGGTTGCCGGGCCGTTGACGTTGGCGTTTGTGGTGATCACCGCCGCGTGGTTGTTCTTTCCGCCGCTGTTGAAGACTGGTGCGGTGGCCGCAGTTATCGGGGAATGTAAGGCGGCGGCGGGGTATGTGAAATGA

Coding sequence (CDS)

ATGGCGGCGGATCTCTACACCTTACTCAAAATCTCCGCCGCCGTTCTTGCCTCTTTACTCTACTCCTACTTCATCTCCACCAAGCTCCCCAAAGGTCTCCCAAGGCTTCTCTCTCTCCTCCCAATCCTCTCTCTCTACACCCTCCTCCCTCTCCCTCTCTCCTCCCTCCTCCTCGCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTTCTGTTCTGCTTCGATTCCGGCCCTCTATTTACCGACCCACCATTGTCATTCCCTCTGTTCGTCTCAATCGCTTGTTTCCCGATCAGAATCAAGCAAAATCAGCCCGACCCAGATCCTCAAAATCCTCAAAAACCCACGGATCCACCGTTGAAGACCATCGATTCCCCCAAATTGCCGCTCAATCTGCCGGCGAAGGGGTTTCTCTTCGCCGTCTTGGTCGCCGGCGATGATTTCGCCCAAAATCTGTCCCCGAATGCTATGCTCTGTTTTTACTGTGTGTCTCTGTATTTGTTTATTGATGTTGTTTTGGGTCTGTCTAATACGTTTGTGAGATGGGGTTTTGGAATCGAGCTCGAACCGCCGTCGAACGAGCCGTATTTGGCGACGTCGCTCCGGGATTTTTGGGGGAGGAGATGGAATTTGTTGGTTTCGAATACTTTAAGATGGTCTGTATTTTTGCCGGTTAGGGCCGCCGTGAGCGGCGTTTTGGGGCGGCGACGGGCAGCGGTTGTGGCAGTGTTTGCGACGTTTGTGGTGTCGGGGCTGATGCATGAGCTGCTGTTTTACTATGTCACCGGAGCGGTTCCGACTTGGGAGGTTACTGGGTTTTTTGTGCTTCATGGGGTTTGTGTGGTGCTGGAATTTTGGGTGAAGAAGGCTTTGGGAGAAAATTGGCGGCTTCATTGGGCGGTTGCCGGGCCGTTGACGTTGGCGTTTGTGGTGATCACCGCCGCGTGGTTGTTCTTTCCGCCGCTGTTGAAGACTGGTGCGGTGGCCGCAGTTATCGGGGAATGTAAGGCGGCGGCGGGGTATGTGAAATGA

Protein sequence

MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK
Homology
BLAST of Cp4.1LG02g00790 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-64
Identity = 151/350 (43.14%), Postives = 206/350 (58.86%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L  L+K+  + + S+ Y Y+I  ++  G PR LS+ P+L+L+ +LPL  SSL L+ 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           I AFF+TWLA+FKL+LF FD GPL    P +FP F    CFPI+++QN   P  QN    
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI-PIPTNFPRFFCFTCFPIKVQQN---PKSQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
                     PKL   +  K  +FAVL+    + QNLSP  +L  Y V LYL I+++L  
Sbjct: 121 --------HLPKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTF 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
               V    G +LEP SN+PYLATSL+DFWGRRWNL+V   LR +V+ P+R      +  
Sbjct: 181 VKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSS 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKK--ALGE 300
             A    + A F+VSGL+HELLF+Y+   +PT EVT FFVLHGVC  +E  VKK   + +
Sbjct: 241 GWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ 300

Query: 301 NWRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
            WRL   V+  LT+ FV +T  WLF P L ++G +     E      ++K
Sbjct: 301 RWRLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Cp4.1LG02g00790 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.1e-62
Identity = 144/349 (41.26%), Postives = 207/349 (59.31%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L +L+K+    + S+ Y Y+ ST++  G+ RLLS+LP+  L+ +LPL +SS+  +G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
             AFF++WLA+FKL+LF FD GPLF   P +   FV   CFPI+++QN   P PQN    
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF-PVPSNLSRFVCFTCFPIKLQQN---PKPQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
                     PK       K   F VL+   ++ Q++SP  +L  Y + +YL  +++L  
Sbjct: 121 --------QMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAP 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
               +      +LEP  NEPYL+TSL+DFWGRRWNL+V   LR +V+LPVR      +  
Sbjct: 181 LKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNS 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKA-LGEN 300
            +A  + VFA+F+VSG++HEL+F+Y T   PT EVT FFVLHGVC   E   K+  L   
Sbjct: 241 DQALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRR 300

Query: 301 WRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
           W++   V+  LT+ FVV+T  WLFFP L ++G +  +  E     G+VK
Sbjct: 301 WKVSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of Cp4.1LG02g00790 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.0e-60
Identity = 146/351 (41.60%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L   +K+  + + S+ Y Y++ST +  G+ RLLS+LP+ +L+ + PL  S +  +G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
            +AFF++WLA+FKL+LF FD GPL +  P +   F+ I CFPI+ +QN   P+ QN    
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SPLPRTLSRFICITCFPIKPQQN---PNIQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPA-KGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLG 180
                      K+P+ L A K  +F VL+   ++ Q LSP  +L F  + ++L +++V  
Sbjct: 121 ----------YKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFM 180

Query: 181 LSNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLG 240
           L    V    G +LEP SNEPYLATSL+DFWGRRWNL+V   LR +V+LP R      + 
Sbjct: 181 LVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVN 240

Query: 241 RRRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKA--LG 300
             +A  + VFA F+VSG +HE+LF+Y+T  VPT EVT FF+LHGVC V E  VKK+  + 
Sbjct: 241 SDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVR 300

Query: 301 ENWRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
             WR+   V+  LT+ FVV+T+ W FF PL+++G +  +  E      +VK
Sbjct: 301 RWWRVSPTVSRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFVK 332

BLAST of Cp4.1LG02g00790 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 8.0e-58
Identity = 144/335 (42.99%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 8   LLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFIT 67
           L+++  + L SL Y Y+IS+KL KG+ RLLS+LP+  L+ +LPL LS +    I   F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 68  WLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTDPPLKT 127
           WLA+FKLLLF FD GPLF  PP     F+  AC PI+I+Q   DP P        P L  
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQ---DPSPN-----AIPNLHP 127

Query: 128 IDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTFVRW 187
              PK    L  K  +  VL+   ++   L    +L  YC+ +YL +++VL      V  
Sbjct: 128 KPMPKWV--LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVST 187

Query: 188 GFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAAVVA 247
             G  +EP  NEPYLATSL+DFW RRWNL+VS  LR +V +PV+     +L    A    
Sbjct: 188 LLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAG 247

Query: 248 VFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRL--HWA 307
           V A+F VSGLMHELL++Y+    PTWEVT FFVLHG     E  VK+     W    H A
Sbjct: 248 VMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRA 307

Query: 308 VAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGEC 341
           V+G + L FV +T  WLF   +L+       IGEC
Sbjct: 308 VSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGEC 328

BLAST of Cp4.1LG02g00790 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 128/341 (37.54%), Postives = 198/341 (58.06%), Query Frame = 0

Query: 7   TLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFI 66
           + +K    V+ SL Y++FI+  +PKG+ RL+   P+  ++ ++P  + SL L GI AFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  TWLASFKLLLFCFDSGPLFTD-PPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTDPPL 126
            WLA+FKLLLF    GPL ++  PLS P+F++++C PI+I Q  P P   +  +      
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-QLSPKPTKTHSHE------ 122

Query: 127 KTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTFV 186
               S + PL    K     +++   +++  L    +L  Y + +Y  ++++L  +   V
Sbjct: 123 ---GSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAV 182

Query: 187 RWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAAV 246
           R    +ELEP  N+PYLATSL+DFWGRRWNL+V+  LR +V+ P     S VLG   + +
Sbjct: 183 RAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQI 242

Query: 247 VAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRLHWA 306
           +A F TFVVSG+MHEL+F+Y+    P W++  FF+++G C  +E  +KK +   WR   A
Sbjct: 243 LAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKA 302

Query: 307 VAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGY 347
           ++  LTL FV++TA WLF P   +   V   + E  A   +
Sbjct: 303 ISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

BLAST of Cp4.1LG02g00790 vs. NCBI nr
Match: XP_023525214.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678 bits (1750), Expect = 1.03e-245
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG
Sbjct: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP
Sbjct: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
           TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL
Sbjct: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
           SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR
Sbjct: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW 300
           RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW
Sbjct: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW 300

Query: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK
Sbjct: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348

BLAST of Cp4.1LG02g00790 vs. NCBI nr
Match: XP_022981530.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 658 bits (1698), Expect = 8.68e-238
Identity = 335/348 (96.26%), Postives = 343/348 (98.56%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           MAADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG
Sbjct: 1   MAADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           IVAFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSIAC+PIRIKQNQ DPDPQNPQKP
Sbjct: 61  IVAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKP 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
           TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPN MLCFYCVSLYLFIDVVLG+
Sbjct: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGI 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
            NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGV+GR
Sbjct: 181 CNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGR 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW 300
           RRAAVVAVFATFVVSGLMHEL+FYYVTGA+PTWEVTGFFVLHGVCVV+EFWVK ALGENW
Sbjct: 241 RRAAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENW 300

Query: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           RLHWAVAGPLTLAFVVITAAWLFFPPLLKTG VAAVIGECKAAAGYVK
Sbjct: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAAAGYVK 348

BLAST of Cp4.1LG02g00790 vs. NCBI nr
Match: XP_022940347.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 655 bits (1689), Expect = 1.97e-236
Identity = 334/346 (96.53%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 3   ADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 62
           ADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 63  AFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTD 122
           AFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSI+CFPIRIKQNQPDPDPQNPQKPTD
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 123 PPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSN 182
           P LKTIDSPKL LNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 183 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 242
           TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 243 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRL 302
           AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVV+EFWVK  LGENWRL
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWRL 301

Query: 303 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAA+IGECKAAAGYVK
Sbjct: 302 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 347

BLAST of Cp4.1LG02g00790 vs. NCBI nr
Match: KAG6607778.1 (putative long-chain-alcohol O-fatty-acyltransferase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 632 bits (1631), Expect = 4.62e-227
Identity = 327/344 (95.06%), Postives = 332/344 (96.51%), Query Frame = 0

Query: 8   LLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFIT 67
           LLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSS LLAGIVAFFIT
Sbjct: 37  LLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSPLLAGIVAFFIT 96

Query: 68  WLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQ---KPTDPP 127
           WLASFKLLLF FDSGPLF+DPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQ   KPTD  
Sbjct: 97  WLASFKLLLFFFDSGPLFSDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQNPQKPTDSL 156

Query: 128 LKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTF 187
           LKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLY FIDV+LGL NTF
Sbjct: 157 LKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCNTF 216

Query: 188 VRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAA 247
           VRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAA
Sbjct: 217 VRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAA 276

Query: 248 VVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRLHW 307
           VVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVV+EFW K ALGENWRLHW
Sbjct: 277 VVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWAKMALGENWRLHW 336

Query: 308 AVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           AVA PLTLAFVVITAAWLFFPPLLKTGAVAA+IGECKAAAGYVK
Sbjct: 337 AVAAPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 380

BLAST of Cp4.1LG02g00790 vs. NCBI nr
Match: XP_022940348.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 [Cucurbita moschata])

HSP 1 Score: 549 bits (1415), Expect = 1.49e-195
Identity = 282/291 (96.91%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 3   ADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 62
           ADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 63  AFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTD 122
           AFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSI+CFPIRIKQNQPDPDPQNPQKPTD
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 123 PPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSN 182
           P LKTIDSPKL LNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 183 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 242
           TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 243 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVK 293
           AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVV+EFWVK
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVK 292

BLAST of Cp4.1LG02g00790 vs. ExPASy TrEMBL
Match: A0A6J1IZR7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480619 PE=3 SV=1)

HSP 1 Score: 658 bits (1698), Expect = 4.20e-238
Identity = 335/348 (96.26%), Postives = 343/348 (98.56%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           MAADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG
Sbjct: 1   MAADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           IVAFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSIAC+PIRIKQNQ DPDPQNPQKP
Sbjct: 61  IVAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKP 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
           TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPN MLCFYCVSLYLFIDVVLG+
Sbjct: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGI 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
            NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGV+GR
Sbjct: 181 CNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGR 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW 300
           RRAAVVAVFATFVVSGLMHEL+FYYVTGA+PTWEVTGFFVLHGVCVV+EFWVK ALGENW
Sbjct: 241 RRAAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENW 300

Query: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           RLHWAVAGPLTLAFVVITAAWLFFPPLLKTG VAAVIGECKAAAGYVK
Sbjct: 301 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAAAGYVK 348

BLAST of Cp4.1LG02g00790 vs. ExPASy TrEMBL
Match: A0A6J1FJC3 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 655 bits (1689), Expect = 9.53e-237
Identity = 334/346 (96.53%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 3   ADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 62
           ADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 63  AFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTD 122
           AFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSI+CFPIRIKQNQPDPDPQNPQKPTD
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 123 PPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSN 182
           P LKTIDSPKL LNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 183 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 242
           TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 243 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRL 302
           AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVV+EFWVK  LGENWRL
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWRL 301

Query: 303 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAA+IGECKAAAGYVK
Sbjct: 302 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 347

BLAST of Cp4.1LG02g00790 vs. ExPASy TrEMBL
Match: A0A6J1FP14 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 549 bits (1415), Expect = 7.22e-196
Identity = 282/291 (96.91%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 3   ADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 62
           ADLYTLLKISAAVLASLLYSYFIS+KLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 63  AFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTD 122
           AFFITWLASFKLLLFCFDSGPLF+DPPLSFPLFVSI+CFPIRIKQNQPDPDPQNPQKPTD
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 123 PPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSN 182
           P LKTIDSPKL LNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 183 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 242
           TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 243 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVK 293
           AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVV+EFWVK
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVK 292

BLAST of Cp4.1LG02g00790 vs. ExPASy TrEMBL
Match: A0A6J1CCA4 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Momordica charantia OX=3673 GN=LOC111010365 PE=3 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 4.11e-163
Identity = 255/349 (73.07%), Postives = 279/349 (79.94%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           MAA+L+ L+K+S  V ASLLYSYF+++KLPKG  RLLSLLPI  L+ LLP      LL+G
Sbjct: 1   MAAELHALIKVSLCVFASLLYSYFVASKLPKGKFRLLSLLPIFPLFALLPX-----LLSG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           IVAFFITWLASFKLL F FDSGPL +DPPL FPLF SIACFPIRIKQN P        K 
Sbjct: 61  IVAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPP--------KT 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
             PP    DSPKLPLNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDVVLGL
Sbjct: 121 LHPPPHLPDSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGL 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
           SN  VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR+ V+ PVRAA    LGR
Sbjct: 181 SNAIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYXVYRPVRAA----LGR 240

Query: 241 RR-AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGEN 300
           RR AA  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVV+EF VK+A+GE 
Sbjct: 241 RRGAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEK 300

Query: 301 WRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348
           WRLHWAV+GPLT+AFVV+T AWLFFPPLL+TGAV  V+ ECK+AA +VK
Sbjct: 301 WRLHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 332

BLAST of Cp4.1LG02g00790 vs. ExPASy TrEMBL
Match: A0A0A0K8E9 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=3 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 2.93e-142
Identity = 223/340 (65.59%), Postives = 265/340 (77.94%), Query Frame = 0

Query: 3   ADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 62
           ADL+ LLKI  +VLASLLYSYFIS+KLPKGLPRLLSLLPILSL+TLLPL +SS+L A IV
Sbjct: 6   ADLFALLKIFLSVLASLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLLVSSILFAAIV 65

Query: 63  AFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTD 122
           +  +TWL SFKLLLF F SGPL +DPPL F LFVS+A FPIRIK+ +        QKP  
Sbjct: 66  SSIVTWLTSFKLLLFSFHSGPLVSDPPLPFLLFVSLAFFPIRIKKKES-------QKP-- 125

Query: 123 PPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSN 182
                IDSPKLP+N PAK FLF VL+A +D   +L P   LCF CV+LY FID+V+G  N
Sbjct: 126 -----IDSPKLPINFPAKVFLFTVLMAVED---SLPPYMKLCFNCVALYFFIDIVVGFFN 185

Query: 183 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 242
           T  RW FGIE E PSNEPYL+TSL+DFWGRRWN+LVSN LR +VF+PVR+A+  V GRRR
Sbjct: 186 TIARWVFGIEFEEPSNEPYLSTSLQDFWGRRWNILVSNLLRLTVFIPVRSAMIDVAGRRR 245

Query: 243 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRL 302
           +A V VFA FVVSGLMHELLFYYV    P+WEVTGFFVL GVCV+LEF +K A+GE +R+
Sbjct: 246 SAAVGVFAVFVVSGLMHELLFYYVNRESPSWEVTGFFVLQGVCVLLEFQLKMAVGEKFRM 305

Query: 303 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKA 342
           H+AV   LT+ FVV+TA+WLFFPP+L+ G VA + G+C+A
Sbjct: 306 HYAVCWVLTMGFVVVTASWLFFPPVLRNGTVARLTGDCRA 328

BLAST of Cp4.1LG02g00790 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.4e-65
Identity = 151/350 (43.14%), Postives = 206/350 (58.86%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L  L+K+  + + S+ Y Y+I  ++  G PR LS+ P+L+L+ +LPL  SSL L+ 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
           I AFF+TWLA+FKL+LF FD GPL    P +FP F    CFPI+++QN   P  QN    
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI-PIPTNFPRFFCFTCFPIKVQQN---PKSQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
                     PKL   +  K  +FAVL+    + QNLSP  +L  Y V LYL I+++L  
Sbjct: 121 --------HLPKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTF 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
               V    G +LEP SN+PYLATSL+DFWGRRWNL+V   LR +V+ P+R      +  
Sbjct: 181 VKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSS 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKK--ALGE 300
             A    + A F+VSGL+HELLF+Y+   +PT EVT FFVLHGVC  +E  VKK   + +
Sbjct: 241 GWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ 300

Query: 301 NWRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
            WRL   V+  LT+ FV +T  WLF P L ++G +     E      ++K
Sbjct: 301 RWRLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Cp4.1LG02g00790 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.9e-63
Identity = 144/349 (41.26%), Postives = 207/349 (59.31%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L +L+K+    + S+ Y Y+ ST++  G+ RLLS+LP+  L+ +LPL +SS+  +G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
             AFF++WLA+FKL+LF FD GPLF   P +   FV   CFPI+++QN   P PQN    
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF-PVPSNLSRFVCFTCFPIKLQQN---PKPQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGL 180
                     PK       K   F VL+   ++ Q++SP  +L  Y + +YL  +++L  
Sbjct: 121 --------QMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAP 180

Query: 181 SNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGR 240
               +      +LEP  NEPYL+TSL+DFWGRRWNL+V   LR +V+LPVR      +  
Sbjct: 181 LKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNS 240

Query: 241 RRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKA-LGEN 300
            +A  + VFA+F+VSG++HEL+F+Y T   PT EVT FFVLHGVC   E   K+  L   
Sbjct: 241 DQALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRR 300

Query: 301 WRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
           W++   V+  LT+ FVV+T  WLFFP L ++G +  +  E     G+VK
Sbjct: 301 WKVSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of Cp4.1LG02g00790 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.2e-62
Identity = 146/351 (41.60%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 1   MAADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAG 60
           M  +L   +K+  + + S+ Y Y++ST +  G+ RLLS+LP+ +L+ + PL  S +  +G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  IVAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKP 120
            +AFF++WLA+FKL+LF FD GPL +  P +   F+ I CFPI+ +QN   P+ QN    
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SPLPRTLSRFICITCFPIKPQQN---PNIQN---- 120

Query: 121 TDPPLKTIDSPKLPLNLPA-KGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLG 180
                      K+P+ L A K  +F VL+   ++ Q LSP  +L F  + ++L +++V  
Sbjct: 121 ----------YKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFM 180

Query: 181 LSNTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLG 240
           L    V    G +LEP SNEPYLATSL+DFWGRRWNL+V   LR +V+LP R      + 
Sbjct: 181 LVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVN 240

Query: 241 RRRAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKA--LG 300
             +A  + VFA F+VSG +HE+LF+Y+T  VPT EVT FF+LHGVC V E  VKK+  + 
Sbjct: 241 SDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVR 300

Query: 301 ENWRLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 349
             WR+   V+  LT+ FVV+T+ W FF PL+++G +  +  E      +VK
Sbjct: 301 RWWRVSPTVSRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFVK 332

BLAST of Cp4.1LG02g00790 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 225.7 bits (574), Expect = 5.7e-59
Identity = 144/335 (42.99%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 8   LLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFIT 67
           L+++  + L SL Y Y+IS+KL KG+ RLLS+LP+  L+ +LPL LS +    I   F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 68  WLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTDPPLKT 127
           WLA+FKLLLF FD GPLF  PP     F+  AC PI+I+Q   DP P        P L  
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQ---DPSPN-----AIPNLHP 127

Query: 128 IDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTFVRW 187
              PK    L  K  +  VL+   ++   L    +L  YC+ +YL +++VL      V  
Sbjct: 128 KPMPKWV--LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVST 187

Query: 188 GFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAAVVA 247
             G  +EP  NEPYLATSL+DFW RRWNL+VS  LR +V +PV+     +L    A    
Sbjct: 188 LLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAG 247

Query: 248 VFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRL--HWA 307
           V A+F VSGLMHELL++Y+    PTWEVT FFVLHG     E  VK+     W    H A
Sbjct: 248 VMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRA 307

Query: 308 VAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGEC 341
           V+G + L FV +T  WLF   +L+       IGEC
Sbjct: 308 VSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGEC 328

BLAST of Cp4.1LG02g00790 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-58
Identity = 128/341 (37.54%), Postives = 198/341 (58.06%), Query Frame = 0

Query: 7   TLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIVAFFI 66
           + +K    V+ SL Y++FI+  +PKG+ RL+   P+  ++ ++P  + SL L GI AFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  TWLASFKLLLFCFDSGPLFTD-PPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPTDPPL 126
            WLA+FKLLLF    GPL ++  PLS P+F++++C PI+I Q  P P   +  +      
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-QLSPKPTKTHSHE------ 122

Query: 127 KTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLSNTFV 186
               S + PL    K     +++   +++  L    +L  Y + +Y  ++++L  +   V
Sbjct: 123 ---GSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAV 182

Query: 187 RWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRRAAV 246
           R    +ELEP  N+PYLATSL+DFWGRRWNL+V+  LR +V+ P     S VLG   + +
Sbjct: 183 RAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQI 242

Query: 247 VAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWRLHWA 306
           +A F TFVVSG+MHEL+F+Y+    P W++  FF+++G C  +E  +KK +   WR   A
Sbjct: 243 LAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKA 302

Query: 307 VAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGY 347
           ++  LTL FV++TA WLF P   +   V   + E  A   +
Sbjct: 303 ISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJ762.0e-6443.14Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Q9FJ754.1e-6241.26Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX... [more]
Q9FJ741.0e-6041.60Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX... [more]
Q9FJ728.0e-5842.99Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9SV071.8e-5737.54Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Match NameE-valueIdentityDescription
XP_023525214.11.03e-245100.00probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep... [more]
XP_022981530.18.68e-23896.26probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima][more]
XP_022940347.11.97e-23696.53probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita mosc... [more]
KAG6607778.14.62e-22795.06putative long-chain-alcohol O-fatty-acyltransferase 5, partial [Cucurbita argyro... [more]
XP_022940348.11.49e-19596.91probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
A0A6J1IZR74.20e-23896.26probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366... [more]
A0A6J1FJC39.53e-23796.53probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita mo... [more]
A0A6J1FP147.22e-19696.91probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita mo... [more]
A0A6J1CCA44.11e-16373.07LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Mo... [more]
A0A0A0K8E92.93e-14265.59MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=... [more]
Match NameE-valueIdentityDescription
AT5G55340.11.4e-6543.14MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55350.12.9e-6341.26MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55360.17.2e-6241.60MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.15.7e-5942.99MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.11.3e-5837.54acyl-CoA sterol acyl transferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017088Wax synthase, MagnoliopsidaPIRSFPIRSF037006Wax_synthasecoord: 1..348
e-value: 1.5E-120
score: 400.5
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 197..281
e-value: 1.1E-14
score: 54.4
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 1..347
NoneNo IPR availablePANTHERPTHR31595:SF8(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 1..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g00790.1Cp4.1LG02g00790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity